Nsf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nsf
Entrez Gene ID	18195
Full Name	N-ethylmaleimide sensitive fusion protein
Synonyms	AI316878,AU020090,AU067812,SKD2
General protein information	Preferred Names N-ethylmaleimide sensitive fusion protein Names vesicle-fusing ATPase N-ethylmaleimide sensitive factor NEM-sensitive fusion protein suppressor of K(+) transport growth defect 2 vesicular-fusion protein NSF
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 E1\|11 67.54 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008740.4	NP_032766.2	vesicle-fusing ATPase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NSF	XP_511626.3
Canis lupus familiaris (dog)	NSF	XP_548044.2
Mus musculus (house mouse)	Nsf	NP_032766.2
Drosophila melanogaster (fruit fly)	comt	NP_001259506.1
Saccharomyces cerevisiae (baker's yeast)	SEC18	NP_009636.3
Gallus gallus (chicken)	NSF	NP_001019627.1
Danio rerio (zebrafish)	nsfa	NP_001037793.1
Xenopus tropicalis (tropical clawed frog)	nsf	NP_001072788.1
Neurospora crassa	NCU03387	XP_956834.1
Bos taurus (cattle)	NSF	XP_002696103.1
Schizosaccharomyces pombe (fission yeast)	sec18	NP_594690.1
Magnaporthe oryzae (rice blast fungus)	MGG_02418	XP_003709204.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012684	XP_307148.3
Macaca mulatta (Rhesus monkey)	NSF	XP_002800596.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076603.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076604.1
Kluyveromyces lactis	KLLA0C14520g	XP_452850.1
Eremothecium gossypii	AGOS_AER169C	NP_985027.1
Homo sapiens (human)	NSF	NP_006169.2
Rattus norvegicus (Norway rat)	Nsf	NP_068516.1
Drosophila melanogaster (fruit fly)	Nsf2	NP_788676.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
Li J, Zhang W, Yang H, Howrigan DP, Wilkinson B, Souaiaia T, Evgrafov OV, Genovese G, Clementel VA, Tudor JC, Abel T, Knowles JA, Neale BM, Wang K, Sun F, Coba MP
Nature neuroscience20(8)1150-1161(2017 Aug)

Munc13-1 and Munc18-1 together prevent NSF-dependent de-priming of synaptic vesicles.
He E, Wierda K, van Westen R, Broeke JH, Toonen RF, Cornelisse LN, Verhage M
Nature communications815915(2017 06)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

N-Ethylmaleimide Sensitive Factor (NSF) Inhibition Prevents Vascular Instability following Gram-Positive Pulmonary Challenge.
Lee JY, Linge HM, Ochani K, Lin K, Miller EJ
PloS one11(6)e0157837(2016)

LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate.
Belluzzi E, Gonnelli A, Cirnaru MD, Marte A, Plotegher N, Russo I, Civiero L, Cogo S, Carrion MP, Franchin C, Arrigoni G, Beltramini M, Bubacco L, Onofri F, Piccoli G, Greggio E
Molecular neurodegeneration111(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nsf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nsf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16089	NM_008740.4 Latest version!	Mus musculus N-ethylmaleimide sensitive fusion protein (Nsf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$433.30 $619.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nsf ( NM_008740.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032766.2 Mus musculus N-ethylmaleimide sensitive fusion protein (Nsf), mRNA.

Price & Availability↑

CloneID	OMu16089
Clone ID Related Accession (Same CDS sequence)	NM_008740.4
Accession Version	NM_008740.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	vesicle-fusing ATPase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL603829.5 and AL596108.13. On Apr 27, 2007 this sequence version replaced NM_008740.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006627.1, SRR1660815.175815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGGCC GGACTATGCA AGCTGCGAGA TGCCCTACTG ATGAATTATC TTTAAGCAAC 
TGTGCGGTTG TGAACGAAAA AGATTTCCAG TCTGGCCAGC ATGTGATGGT GAGGACGTCC 
CCCAATCACA AGTACATATT TACGCTGAGG ACCCATCCGT CAGTGGTTCC AGGATGCATC 
GCGTTCAGCT TGCCTCAGCG AAAATGGGCT GGACTTTCTA TTGGACAGGA CATAGAAGTT 
GCCTTGTATT CGTTTGACAA AGCCAAGCAG TGCATCGGCA CAATGACCAT CGAGATCGAC 
TTCCTGCAGA AGAAGAACAT CGACTCCAAC CCTTACGACA CCGACAAGAT GGCCGCCGAG 
TTCATCCAGC AGTTCAACAA CCAGGCCTTC TCAGTGGGGC AGCAGCTTGT CTTTAGCTTC 
AATGATAAGC TCTTTGGATT ACTGGTGAAG GACATTGAAG CCATGGATCC CAGCATCCTG 
AAGGGAGAGC CTGCATCAGG CAAAAGGCAG AAGATTGAGG TAGGCCTGGT TGTTGGAAAC 
AGTCAAGTTG CATTTGAAAA AGCAGAAAAT TCGTCGCTCA ATCTTATCGG CAAAGCTAAA 
ACCAAGGAAA ATCGCCAGTC CATCATCAAT CCCGACTGGA ACTTTGAGAA AATGGGGATC 
GGCGGTCTGG ATAAGGAGTT CTCGGACATT TTCCGAAGAG CATTTGCTTC TCGGGTGTTC 
CCCCCGGAGA TCGTGGAACA GATGGGTTGC AAACATGTTA AAGGCATCCT GTTATACGGA 
CCCCCTGGTT GTGGTAAAAC TCTCTTGGCT CGACAGATTG GCAAGATGCT GAATGCGAGA 
GAGCCCAAGG TGGTCAATGG GCCAGAAATC CTTAACAAGT ATGTGGGAGA ATCAGAGGCT 
AACATTCGTA AACTCTTTGC TGATGCCGAA GAGGAGCAAA GGAGGCTTGG TGCTAACAGT 
GGCTTGCACA TCATCATCTT TGATGAAATC GATGCCATCT GCAAGCAGAG AGGGAGCATG 
GCCGGCAGCA CTGGAGTGCA TGACACCGTG GTCAACCAGC TGCTGTCCAA GATCGATGGC 
GTTGAGCAGC TGAACAACAT CCTCGTTATC GGAATGACCA ATAGACCAGA TTTGATAGAC 
GAAGCTCTCC TTCGACCCGG AAGACTGGAA GTTAAAATGG AGATAGGCTT GCCAGATGAG 
AAGGGTCGAC TCCAGATCCT TCATATCCAC ACAGCAAGGA TGAGAGGGCA CCAGTTACTG 
TCTGCAGATG TGGACATCAA GGAGCTGGCT GTGGAGACTA AGAATTTCAG TGGCGCTGAG 
CTGGAGGGTC TGGTGCGAGC AGCGCAGTCC ACAGCCATGA ACAGACATAT CAAGGCCAGC 
ACCAAAGTAG AAGTAGACAT GGAGAAGGCA GAGAGCCTGC AGGTGACAAG AGGGGACTTC 
CTTGCTTCCC TGGAGAACGA TATCAAACCA GCATTTGGCA CCAACCAAGA GGATTATGCA 
AGTTACATTA TGAATGGGAT CATCAAGTGG GGTGACCCCG TGACCCGAGT CCTAGATGAC 
GGAGAGCTGC TGGTCCAGCA AACGAAAAAC AGTGACCGGA CACCACTCGT TAGTGTCCTC 
CTAGAAGGGC CTCCTCACAG CGGGAAGACT GCCCTGGCTG CAAAGATTGC CGAGGAATCC 
AACTTCCCCT TCATCAAGAT CTGCTCACCT GATAAGATGA TTGGCTTCTC CGAGACAGCC 
AAGTGTCAGG CCATGAAGAA GATCTTTGAT GATGCTTACA AATCTCAGCT CAGCTGTGTG 
GTCGTGGATG ACATTGAGAG GCTGCTCGAT TATGTCCCCA TTGGTCCCCG GTTCTCCAAC 
CTGGTATTGC AGGCTCTTCT TGTGTTACTG AAAAAGGCGC CTCCTCAGGG CCGCAAGCTT 
CTTATCATCG GGACCACCAG CCGCAAAGAT GTCCTGCAGG AAATGGAGAT GCTGAACGCC 
TTCAGCACCA CCATCCACGT GCCCAACATT GCCACAGGAG AGCAGCTGCT GGAAGCTCTG 
GAGCTTTTGG GCAACTTCAA AGATAAGGAA CGCACCACAA TTGCTCAGCA AGTCAAAGGG 
AAGAAAGTCT GGATAGGAAT CAAGAAGTTA CTAATGTTGA TTGAGATGTC CCTGCAGATG 
GATCCTGAGT ACCGTGTGAG GAAATTCTTG GCCCTCATGA GAGAAGAAGG CGCTAGTCCC 
CTGGACTTTG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032766.2
CDS	76..2310
Translation	MAGRTMQAARCPTDELSLSNCAVVNEKDFQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASPLDFD

Target ORF information:

RefSeq Version	NM_008740.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-ethylmaleimide sensitive fusion protein (Nsf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008740.4

ORF Insert Sequence:

ATGGCGGGCC GGACTATGCA AGCTGCGAGA TGCCCTACTG ATGAATTATC TTTAAGCAAC 
TGTGCGGTTG TGAACGAAAA AGATTTCCAG TCTGGCCAGC ATGTGATGGT GAGGACGTCC 
CCCAATCACA AGTACATATT TACGCTGAGG ACCCATCCGT CAGTGGTTCC AGGATGCATC 
GCGTTCAGCT TGCCTCAGCG AAAATGGGCT GGACTTTCTA TTGGACAGGA CATAGAAGTT 
GCCTTGTATT CGTTTGACAA AGCCAAGCAG TGCATCGGCA CAATGACCAT CGAGATCGAC 
TTCCTGCAGA AGAAGAACAT CGACTCCAAC CCTTACGACA CCGACAAGAT GGCCGCCGAG 
TTCATCCAGC AGTTCAACAA CCAGGCCTTC TCAGTGGGGC AGCAGCTTGT CTTTAGCTTC 
AATGATAAGC TCTTTGGATT ACTGGTGAAG GACATTGAAG CCATGGATCC CAGCATCCTG 
AAGGGAGAGC CTGCATCAGG CAAAAGGCAG AAGATTGAGG TAGGCCTGGT TGTTGGAAAC 
AGTCAAGTTG CATTTGAAAA AGCAGAAAAT TCGTCGCTCA ATCTTATCGG CAAAGCTAAA 
ACCAAGGAAA ATCGCCAGTC CATCATCAAT CCCGACTGGA ACTTTGAGAA AATGGGGATC 
GGCGGTCTGG ATAAGGAGTT CTCGGACATT TTCCGAAGAG CATTTGCTTC TCGGGTGTTC 
CCCCCGGAGA TCGTGGAACA GATGGGTTGC AAACATGTTA AAGGCATCCT GTTATACGGA 
CCCCCTGGTT GTGGTAAAAC TCTCTTGGCT CGACAGATTG GCAAGATGCT GAATGCGAGA 
GAGCCCAAGG TGGTCAATGG GCCAGAAATC CTTAACAAGT ATGTGGGAGA ATCAGAGGCT 
AACATTCGTA AACTCTTTGC TGATGCCGAA GAGGAGCAAA GGAGGCTTGG TGCTAACAGT 
GGCTTGCACA TCATCATCTT TGATGAAATC GATGCCATCT GCAAGCAGAG AGGGAGCATG 
GCCGGCAGCA CTGGAGTGCA TGACACCGTG GTCAACCAGC TGCTGTCCAA GATCGATGGC 
GTTGAGCAGC TGAACAACAT CCTCGTTATC GGAATGACCA ATAGACCAGA TTTGATAGAC 
GAAGCTCTCC TTCGACCCGG AAGACTGGAA GTTAAAATGG AGATAGGCTT GCCAGATGAG 
AAGGGTCGAC TCCAGATCCT TCATATCCAC ACAGCAAGGA TGAGAGGGCA CCAGTTACTG 
TCTGCAGATG TGGACATCAA GGAGCTGGCT GTGGAGACTA AGAATTTCAG TGGCGCTGAG 
CTGGAGGGTC TGGTGCGAGC AGCGCAGTCC ACAGCCATGA ACAGACATAT CAAGGCCAGC 
ACCAAAGTAG AAGTAGACAT GGAGAAGGCA GAGAGCCTGC AGGTGACAAG AGGGGACTTC 
CTTGCTTCCC TGGAGAACGA TATCAAACCA GCATTTGGCA CCAACCAAGA GGATTATGCA 
AGTTACATTA TGAATGGGAT CATCAAGTGG GGTGACCCCG TGACCCGAGT CCTAGATGAC 
GGAGAGCTGC TGGTCCAGCA AACGAAAAAC AGTGACCGGA CACCACTCGT TAGTGTCCTC 
CTAGAAGGGC CTCCTCACAG CGGGAAGACT GCCCTGGCTG CAAAGATTGC CGAGGAATCC 
AACTTCCCCT TCATCAAGAT CTGCTCACCT GATAAGATGA TTGGCTTCTC CGAGACAGCC 
AAGTGTCAGG CCATGAAGAA GATCTTTGAT GATGCTTACA AATCTCAGCT CAGCTGTGTG 
GTCGTGGATG ACATTGAGAG GCTGCTCGAT TATGTCCCCA TTGGTCCCCG GTTCTCCAAC 
CTGGTATTGC AGGCTCTTCT TGTGTTACTG AAAAAGGCGC CTCCTCAGGG CCGCAAGCTT 
CTTATCATCG GGACCACCAG CCGCAAAGAT GTCCTGCAGG AAATGGAGAT GCTGAACGCC 
TTCAGCACCA CCATCCACGT GCCCAACATT GCCACAGGAG AGCAGCTGCT GGAAGCTCTG 
GAGCTTTTGG GCAACTTCAA AGATAAGGAA CGCACCACAA TTGCTCAGCA AGTCAAAGGG 
AAGAAAGTCT GGATAGGAAT CAAGAAGTTA CTAATGTTGA TTGAGATGTC CCTGCAGATG 
GATCCTGAGT ACCGTGTGAG GAAATTCTTG GCCCTCATGA GAGAAGAAGG CGCTAGTCCC 
CTGGACTTTG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders.
Nature neuroscience20(8)1150-1161(2017 Aug)
Li J,Zhang W,Yang H,Howrigan DP,Wilkinson B,Souaiaia T,Evgrafov OV,Genovese G,Clementel VA,Tudor JC,Abel T,Knowles JA,Neale BM,Wang K,Sun F,Coba MP

Munc13-1 and Munc18-1 together prevent NSF-dependent de-priming of synaptic vesicles.
Nature communications815915(2017 06)
He E,Wierda K,van Westen R,Broeke JH,Toonen RF,Cornelisse LN,Verhage M

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

N-Ethylmaleimide Sensitive Factor (NSF) Inhibition Prevents Vascular Instability following Gram-Positive Pulmonary Challenge.
PloS one11(6)e0157837(2016)
Lee JY,Linge HM,Ochani K,Lin K,Miller EJ

LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate.
Molecular neurodegeneration111(2016 Jan)
Belluzzi E,Gonnelli A,Cirnaru MD,Marte A,Plotegher N,Russo I,Civiero L,Cogo S,Carrion MP,Franchin C,Arrigoni G,Beltramini M,Bubacco L,Onofri F,Piccoli G,Greggio E

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Nsf cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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