Ncf4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncf4
Entrez Gene ID	17972
Full Name	neutrophil cytosolic factor 4
Synonyms	AI451400,p40phox
General protein information	Preferred Names neutrophil cytosolic factor 4 Names neutrophil cytosol factor 4 NCF-4 neutrophil NADPH oxidase factor 4 p40-phox
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 E1\|15 37.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006520566.2	XP_006520629.1	neutrophil cytosol factor 4 isoform X2
XM_030248373.1	XP_030104233.1	neutrophil cytosol factor 4 isoform X2
XM_006520565.2	XP_006520628.1	neutrophil cytosol factor 4 isoform X1
NM_008677.2	NP_032703.2	neutrophil cytosol factor 4
XM_006520564.1	XP_006520627.1	neutrophil cytosol factor 4 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NCF4	NP_001034361.1
Xenopus tropicalis (tropical clawed frog)	ncf4	NP_001025692.1
Mus musculus (house mouse)	Ncf4	NP_032703.2
Homo sapiens (human)	NCF4	NP_000622.2
Rattus norvegicus (Norway rat)	Ncf4	NP_001120776.1
Danio rerio (zebrafish)	ncf4	NP_991274.2
Pan troglodytes (chimpanzee)	NCF4	XP_001159332.2
Canis lupus familiaris (dog)	NCF4	XP_538398.2
Bos taurus (cattle)	NCF4	NP_001039448.1

Gene Ontology
Bibliography

Related articles in PubMed

PI(3)P-p40phox binding regulates NADPH oxidase activation in mouse macrophages and magnitude of inflammatory responses in vivo.
Bagaitkar J, Barbu EA, Perez-Zapata LJ, Austin A, Huang G, Pallat S, Dinauer MC
Journal of leukocyte biology101(2)449-457(2017 02)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst.
Graham DB, Becker CE, Doan A, Goel G, Villablanca EJ, Knights D, Mok A, Ng ACY, Doench JG, Root DE, Clish CB, Xavier RJ
Nature communications67838(2015 Jul)

Role of TNF receptor-associated factor 3 in the CD40 signaling by production of reactive oxygen species through association with p40phox, a cytosolic subunit of nicotinamide adenine dinucleotide phosphate oxidase.
Ha YJ, Lee JR
Journal of immunology (Baltimore, Md. : 1950)172(1)231-9(2004 Jan)

Developmental expression of NADPH phagocytic oxidase components in mouse embryos.
Baehner RL, Millar-Groff S, Bringas P
Pediatric research46(2)152-7(1999 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ncf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20285	NM_008677.2 Latest version!	Mus musculus neutrophil cytosolic factor 4 (Ncf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu20285	XM_006520565.2 Latest version!	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu20285	XM_006520564.1 Latest version!	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu51032	XM_030248373.1 Latest version!	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu51032	XM_006520566.2 Latest version!	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncf4 ( NM_008677.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032703.2 Mus musculus neutrophil cytosolic factor 4 (Ncf4), mRNA.

Price & Availability↑

CloneID	OMu20285
Clone ID Related Accession (Same CDS sequence)	XM_006520565.2 , NM_008677.2 , XM_006520564.1
Accession Version	NM_008677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB840090.1, AK150510.1 and AA016555.1. On Jul 21, 2009 this sequence version replaced NM_008677.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK150510.1, AB002665.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGGCGTGA GTTCCAGAGA 
GAAGACATTG CCCTTAGCTA CCAGGATGCT GAGGGGGACT TGGTTAGGCT GCTGTCAGAT 
GAGGATGTGG GACTCATGGT GAAGCAAGCC CGAGGCCTCC CTTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCATGTCAC ACAGAAGGAC AACTACAGTG TCTACAACAC TGTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032703.2
CDS	246..1265
Translation	MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGVSPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRREFQREDIALSYQDAEGDLVRLLSDEDVGLMVKQARGLPSQKRLFPWKLHVTQKDNYSVYNTVP

Target ORF information:

RefSeq Version	NM_008677.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 4 (Ncf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008677.2

ORF Insert Sequence:

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGGCGTGA GTTCCAGAGA 
GAAGACATTG CCCTTAGCTA CCAGGATGCT GAGGGGGACT TGGTTAGGCT GCTGTCAGAT 
GAGGATGTGG GACTCATGGT GAAGCAAGCC CGAGGCCTCC CTTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCATGTCAC ACAGAAGGAC AACTACAGTG TCTACAACAC TGTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncf4 ( XM_006520565.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520628.1 Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu20285
Clone ID Related Accession (Same CDS sequence)	XM_006520565.2 , NM_008677.2 , XM_006520564.1
Accession Version	XM_006520565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGGCGTGA GTTCCAGAGA 
GAAGACATTG CCCTTAGCTA CCAGGATGCT GAGGGGGACT TGGTTAGGCT GCTGTCAGAT 
GAGGATGTGG GACTCATGGT GAAGCAAGCC CGAGGCCTCC CTTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCATGTCAC ACAGAAGGAC AACTACAGTG TCTACAACAC TGTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520628.1
CDS	22..1041
Translation	MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGVSPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRREFQREDIALSYQDAEGDLVRLLSDEDVGLMVKQARGLPSQKRLFPWKLHVTQKDNYSVYNTVP

Target ORF information:

RefSeq Version	XM_006520565.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520565.2

ORF Insert Sequence:

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGGCGTGA GTTCCAGAGA 
GAAGACATTG CCCTTAGCTA CCAGGATGCT GAGGGGGACT TGGTTAGGCT GCTGTCAGAT 
GAGGATGTGG GACTCATGGT GAAGCAAGCC CGAGGCCTCC CTTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCATGTCAC ACAGAAGGAC AACTACAGTG TCTACAACAC TGTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncf4 ( XM_006520564.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520627.1 Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20285
Clone ID Related Accession (Same CDS sequence)	XM_006520565.2 , NM_008677.2 , XM_006520564.1
Accession Version	XM_006520564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGGCGTGA GTTCCAGAGA 
GAAGACATTG CCCTTAGCTA CCAGGATGCT GAGGGGGACT TGGTTAGGCT GCTGTCAGAT 
GAGGATGTGG GACTCATGGT GAAGCAAGCC CGAGGCCTCC CTTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCATGTCAC ACAGAAGGAC AACTACAGTG TCTACAACAC TGTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520627.1
CDS	110..1129
Translation	MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGVSPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRREFQREDIALSYQDAEGDLVRLLSDEDVGLMVKQARGLPSQKRLFPWKLHVTQKDNYSVYNTVP

Target ORF information:

RefSeq Version	XM_006520564.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520564.1

ORF Insert Sequence:

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGGCGTGA GTTCCAGAGA 
GAAGACATTG CCCTTAGCTA CCAGGATGCT GAGGGGGACT TGGTTAGGCT GCTGTCAGAT 
GAGGATGTGG GACTCATGGT GAAGCAAGCC CGAGGCCTCC CTTCCCAGAA GCGCCTCTTC 
CCCTGGAAGC TGCATGTCAC ACAGAAGGAC AACTACAGTG TCTACAACAC TGTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncf4 ( XM_006520566.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520629.1 Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu51032
Clone ID Related Accession (Same CDS sequence)	XM_006520566.2 , XM_030248373.1
Accession Version	XM_006520566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGAACAGG AGTAAGGCTG 
TTGGATGATG GGCCCCGGTA CCACTTGGAT CCACAGACTC AAAGGCCCTG GGAGAAGCCA 
GGATCCATAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520629.1
CDS	291..1205
Translation	MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGVSPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRTGVRLLDDGPRYHLDPQTQRPWEKPGSID

Target ORF information:

RefSeq Version	XM_006520566.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520566.2

ORF Insert Sequence:

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGAACAGG AGTAAGGCTG 
TTGGATGATG GGCCCCGGTA CCACTTGGAT CCACAGACTC AAAGGCCCTG GGAGAAGCCA 
GGATCCATAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncf4 ( XM_030248373.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104233.1 Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X3, mRNA.

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CloneID	OMu51032
Clone ID Related Accession (Same CDS sequence)	XM_006520566.2 , XM_030248373.1
Accession Version	XM_030248373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGAACAGG AGTAAGGCTG 
TTGGATGATG GGCCCCGGTA CCACTTGGAT CCACAGACTC AAAGGCCCTG GGAGAAGCCA 
GGATCCATAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104233.1
CDS	113..1027
Translation	MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGVSPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRTGVRLLDDGPRYHLDPQTQRPWEKPGSID

Target ORF information:

RefSeq Version	XM_030248373.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 4 (Ncf4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248373.1

ORF Insert Sequence:

ATGGCCCTGG CCCAGCAGCT GCGATCAGAG AGCGACTTTG AGCAGCTTCC AGACGATGTG 
GCCGTCTCAG CCAACATCGC TGACATCGAG GAGAAGAGGG GCTTCACCAG CCACTTTGTT 
TTTGTCATCG AGGTCAAAAC AAAAGGAGGG TCCAAGTATC TCATCTACCG CCGCTATCGC 
CAGTTCTACG CCCTGCAGAG CAAGCTGGAG GAGCGGTTTG GGCCAGAGAG CAAGAACAGC 
CCTTTCACCT GCAGCCTGCC CACATTGCCA GCCAAAGTCT ACATGGGCGC AAAACAAGAG 
ATCGCTGAGA CTCGGATCCC GGCCCTCAAT GCCTACATGA AGAACCTCCT GAGCCTGCCG 
GTCTGCGTGC TGATGGACCC CGACGTCAGG ATCTTCTTCT ATCAGTCTGC ATACGATGCT 
GAGCAGGTGC CCCAGGCACT CCGCAGGCTC CGACCGCGCA CGCGCAAAAT CAAGGGTGTG 
TCTCCACAAG GGGCCATCAT GGATCGCATG GAAGCGCCAA GAGCAGAGGC CTTGTTTGAC 
TTCACTGGGA ACAGCAAATT GGAGCTAAGT TTCAAAGCTG GAGATGTGAT CTTCCTTCTC 
AGTAAGATCA ACAAAGACTG GCTGGAGGGC ACCTCCCAGG GAGCCACAGG CATCTTCCCA 
GGGTCCTTCG TGAAGATCCT TAAGGACTTT CCCGAGGACG AGGACACCAC CAACTGGCTA 
CGATGCTACT TCTATGAAGA CACAGGCAAA ACCATCAAGG ACATTGCGGT GGAGGAGGAC 
CTCAGCAGCA CGCCCCTGTT CAAAGACCTG CTAGCGCTCA TGAGAACAGG AGTAAGGCTG 
TTGGATGATG GGCCCCGGTA CCACTTGGAT CCACAGACTC AAAGGCCCTG GGAGAAGCCA 
GGATCCATAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PI(3)P-p40phox binding regulates NADPH oxidase activation in mouse macrophages and magnitude of inflammatory responses in vivo.
Journal of leukocyte biology101(2)449-457(2017 02)
Bagaitkar J,Barbu EA,Perez-Zapata LJ,Austin A,Huang G,Pallat S,Dinauer MC

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst.
Nature communications67838(2015 Jul)
Graham DB,Becker CE,Doan A,Goel G,Villablanca EJ,Knights D,Mok A,Ng ACY,Doench JG,Root DE,Clish CB,Xavier RJ

Role of TNF receptor-associated factor 3 in the CD40 signaling by production of reactive oxygen species through association with p40phox, a cytosolic subunit of nicotinamide adenine dinucleotide phosphate oxidase.
Journal of immunology (Baltimore, Md. : 1950)172(1)231-9(2004 Jan)
Ha YJ,Lee JR

Developmental expression of NADPH phagocytic oxidase components in mouse embryos.
Pediatric research46(2)152-7(1999 Aug)
Baehner RL,Millar-Groff S,Bringas P

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Ncf4 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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