Myh3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh3
Entrez Gene ID	17883
Full Name	myosin, heavy polypeptide 3, skeletal muscle, embryonic
Synonyms	MyHC-emb,Myhs-e,Myhse
General protein information	Preferred Names myosin, heavy polypeptide 3, skeletal muscle, embryonic Names myosin-3 embryonic skeletal myosin heavy chain isoform myosin heavy chain 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 40.59 cM
Summary	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099635.1	NP_001093105.1	myosin-3
XM_006532412.1	XP_006532475.1	myosin-3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH3	NP_002461.2
Pan troglodytes (chimpanzee)	MYH3	XP_003315696.1
Canis lupus familiaris (dog)	MYH3	NP_001107184.1
Mus musculus (house mouse)	Myh3	NP_001093105.1
Macaca mulatta (Rhesus monkey)	MYH3	XP_001114005.2
Rattus norvegicus (Norway rat)	Myh3	NP_036736.1
Bos taurus (cattle)	MYH3	NP_001095305.1
Xenopus tropicalis (tropical clawed frog)	LOC100495065	XP_002937051.2

Gene Ontology
Bibliography

Related articles in PubMed

The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration.
Sala D, Cunningham TJ, Stec MJ, Etxaniz U, Nicoletti C, Dall'Agnese A, Puri PL, Duester G, Latella L, Sacco A
Nature communications10(1)1796(2019 04)

Deletion of the T-box transcription factor gene, Tbx1, in mice induces differential expression of genes associated with cleft palate in humans.
Funato N, Yanagisawa H
Archives of oral biology95149-155(2018 Nov)

ANKK1 is found in myogenic precursors and muscle fibers subtypes with glycolytic metabolism.
Rubio-Solsona E, Martí S, Vílchez JJ, Palau F, Hoenicka J
PloS one13(5)e0197254(2018)

Clathrin adaptor GGA1 modulates myogenesis of C2C12 myoblasts.
Isobe M, Lee S, Waguri S, Kametaka S
PloS one13(11)e0207533(2018)

HOXA5 plays tissue-specific roles in the developing respiratory system.
Landry-Truchon K, Houde N, Boucherat O, Joncas FH, Dasen JS, Philippidou P, Mansfield JH, Jeannotte L
Development (Cambridge, England)144(19)3547-3561(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Myh3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20095	NM_001099635.1 Latest version!	Mus musculus myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20095	XM_006532412.1 Latest version!	Mus musculus myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh3 ( NM_001099635.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001093105.1 Mus musculus myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3), mRNA.

Price & Availability↑

CloneID	OMu20095
Clone ID Related Accession (Same CDS sequence)	NM_001099635.1 , XM_006532412.1
Accession Version	NM_001099635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	myosin-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645988.6 and AL596129.27. On Jul 25, 2007 this sequence version replaced XM_354614.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTAGCG ACACCGAGAT GGAAGTGTTT GGCATAGCTG CACCTTTCCT CCGCAAGTCG 
GAAAAGGAGA GGATCGAAGC TCAGAACCAG CCCTTTGATG CCAAAACCTA CTGCTTTGTG 
GTTGACTCAA AGGAAGAATA TGTCAAGGGG AAAATTAAGA GTAGCCAGGA TGGGAAAGTC 
ACTGTGGAGA CAGAAGACAG CAGGACCCTG GTGGTTAAGC CAGAGGACGT GTATGCCATG 
AACCCACCCA AGTTTGACAA GATTGAGGAC ATGGCCATGC TGACCCACCT GAACGAGCCC 
GCTGTGCTGT ACAACCTCAA GGACCGATAC ACGTCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAA TGGCTGCCAG TGTACAACCC CGAGGTGGTG 
GACGGCTACC GAGGCAAAAA ACGCCAGGAG GCTCCGCCCC ACATCTTCTC CATCTCCGAC 
AACGCCTACC AGTTCATGCT GACTGATCGT GAGAACCAGT CTATCCTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACGGT GAACACCAAG CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCCACTGGGG ACCTTGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACGCT GGAGGATCAA 
ATTATCAGCG CTAACCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACCGT GAGGAACGAC 
AACTCGTCTC GCTTTGGCAA GTTCATCCGC ATCCATTTTG GCACCACTGG GAAGCTGGCT 
TCTGCAGATA TTGAAACTTA TCTGCTGGAA AAGTCCAGAG TCACCTTCCA GCTGAAGGCT 
GAGAGAAGCT ACCACATCTT CTACCAGATT CTCTCCAACA AGAAACCTGA GCTGATTGAG 
CTGCTGCTGA TTACGACCAA CCCTTATGAC TACCCGTTCA TCAGCCAGGG CGAAATCCTG 
GTGGCCAGCA TCGACGATGC GGAGGAGCTG TTAGCTACGG ATAGTGCCAT CGACATCCTG 
GGCTTCACCC CGGAGGAGAA ATCTGGACTT TATAAACTGA CAGGGGCTGT GATGCATTAT 
GGGAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CCGAGCCAGA TGGCACTGAA 
GTGGCTGACA AAACAGCCTA CTTGATGGGC CTGAATTCCT CAGACCTCCT CAAGGCCTTG 
TGCTTTCCCA GAGTGAAAGT CGGGAATGAA TACGTTACAA AAGGGCAGAC TGTGGACCAG 
GTTCACCATG CTGTGAACGC TCTCTCCAAG TCCGTGTACG AGAAGCTGTT CTTGTGGATG 
GTCACTCGTA TTAACCAGCA GCTGGATACA AAGCTACCAA GACAGCACTT CATTGGCGTT 
TTGGACATTG CGGGTTTTGA AATTTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAACAGGAG 
GAATACAAGA AGGAGGGCAT CGAGTGGACC TTCATCGACT TCGGGATGGA CCTGGCAGCC 
TGCATCGAGC TCATCGAGAA GCCGATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CGACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA CCTGGGAAAG 
TCCAACAACT TCCAGAAACC TAAGGTGGTC AAAGGCAAGG CTGAAGCCCA CTTCTCCCTG 
GTGCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGCT GGCTGGAGAA GAACAAGGAC 
CCCCTGAATG AGACCGTGGT GGGACTGTAC CAGAAGTCCT CCAACAGACT CCTGGCACAT 
CTCTATGCCA CCTTCGCTAC AACAGATGCG GACGGTGGGA AGAAGAAAGT CGCTAAGAAG 
AAAGGCTCTT CCTTCCAAAC CGTCTCTGCA CTGTTCAGGG AAAACTTGAA CAAGCTGATG 
TCCAATTTAA GAACCACACA CCCACACTTT GTACGCTGTA TAATACCCAA CGAAACCAAG 
ACCCCAGGGG CCATGGAACA CAGCCTTGTC CTGCACCAGC TCAGGTGTAA CGGGGTGCTG 
GAAGGCATCC GGATCTGCAG GAAGGGCTTC CCCAACAGGA TCCTGTATGG AGACTTTAAA 
CAGAGATACC GAGTGCTGAA CGCCAGTGCC ATCCCAGAAG GCCAGTTCAT TGACAGCAAG 
AAAGCTTGCG AGAAACTCTT GGCTTCCATT GACATCGACC ACACACAGTA CAAATTTGGA 
CACACCAAGG TGTTCTTTAA GGCTGGCTTG CTAGGAACCC TGGAAGAGAT GCGGGATGAG 
CGTTTGGCCA AACTGATCAC TCGGACACAA GCCGTGTGCA GAGGGTTTCT CATGCGTGTG 
GAATTCCAGA AGATGATGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATCCGT 
GCCTTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTG 
CTGAAGAGCG CGGAGACTGA GAAGGAGATG GCCACCATGA AGGAGGAGTT CCAGAAAACC 
AAGGATGAGC TGGCTAAGTC GGAGGCAAAG AGGAAGGAGC TAGAGGAGAA GCTCGTCACT 
TTGGTACAGG AGAAGAATGA CCTGCAACTT CAAGTACAGG CTGAGAGTGA AAATTTGTTG 
GATGCTGAGG AACGATGCGA CCAGCTGATC AAAGCCAAGT TCCAGCTGGA AGCCAAAATC 
AAAGAGGTGA CAGAGAGAGC CGAGGACGAG GAGGAGATCA ACGCGGAGCT CACGGCCAAG 
AAGAGGAAGC TGGAGGACGA GTGCTCGGAG CTGAAGAAGG ACATCGATGA CCTTGAGCTG 
ACACTGGCCA AGGTTGAGAA GGAGAAGCAC GCCACAGAGA ACAAGGTCAA AAACCTTACC 
GAAGAACTGG CAGGCCTGGA TGAAACTATT GCAAAGCTAA CACGGGAGAA GAAGGCCCTC 
CAAGAGGCCC ACCAGCAGAC CCTGGACGAC CTCCAGGCTG AAGAAGACAA GGTCAATTCT 
CTGAGCAAGC TCAAGAGCAA GCTGGAGCAG CAAGTGGATG ATCTGGAGAG CTCCCTCGAA 
CAAGAAAAGA AGCTGCGTGT GGACCTAGAA AGGAACAAAA GGAAGCTGGA GGGAGACCTG 
AAGCTTGCCC AAGAGTCCAT ATTAGATCTG GAGAACGACA AGCAACAGCT AGACGAGAGG 
CTCAAGAAGA AGGACTTTGA ATATAGTCAG CTGCAAAGCA AAGTGGAAGA TGAGCAGACC 
TTGAGCCTCC AGCTTCAGAA GAAAATCAAG GAGCTACAGG CTCGCATCGA GGAGCTGGAG 
GAAGAGATAG AGGCAGAGAG GGCCACCCGG GCCAAGACAG AGAAGCAGCG CAGCGACTAT 
GCACGCGAGC TGGAGGAGCT GAGTGAGCGG CTGGAGGAGG CGGGCGGTGT CACCTCCACC 
CAGATCGAAC TGAACAAGAA GCGTGAGGCT GAGTTCTTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACCC TGCAGCACGA GGCCACGGTG GCCACGCTGC GGAAGAAGCA CGCGGACAGC 
GCGGCCGAGC TGGCGGAGCA GATCGACAAC CTGCAGCGGG TGAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATTGAC GACCTCTCCA GCAGCGTAGA GAGCGTGTCC 
AAATCCAAGG CCAATCTGGA GAAGATCTGC CGAACTCTAG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAGAT GCAGAGGAGC CTAAGTGAGC TGACCACACA GAAGTCCCGG 
CTGCAGACAG AGGCGGGTGA GCTGAGTCGT CAACTAGAAG AAAAGGAAAG CATTGTATCT 
CAGCTCTCCA GGAGCAAGCA GGCCTTTACC CAACAGATAG AGGAGCTCAA GAGGCAACTG 
GAGGAAGAGA ACAAGGCCAA GAACGCGCTG GCCCACGCCC TGCAGTCCTC CCGCCATGAC 
TGTGACCTGC TGCGGGAACA GTACGAGGAG GAGCAGGAAG GCAAAGCCGA GCTGCAGAGG 
GCACTGTCCA AGGCCAACAG CGAGGTGGCC CAGTGGAGGA CCAAGTATGA AACGGATGCC 
ATCCAGCGCA CGGAGGAGCT GGAGGAGGCC AAAAAAAAGC TTGCGCAGCG CCTTCAGGAT 
TCGGAGGAGC AGGTAGAGGC TGTAAATGCC AAATGTGCTT CCTTGGAAAA GACCAAGCAG 
AGGCTGCAAG GGGAGGTGGA GGACCTGATG GTGGATGTGG AAAGAGCCAA CTCCCTGGCT 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGC TAGCTGAGTG GAAGACGAAG 
TGTGAGGAGA GCCAAGCCGA GCTGGAGGCA GCTCTCAAGG AATCCCGGTC CTTGAGCACT 
GAGCTCTTCA AACTGAAGAA TGCCTATGAA GAAGCCTTGG ATCAACTTGA AACCGTGAAA 
CGGGAGAATA AGAACTTAGA ACAAGAGATA GCAGATCTCA CTGAACAGAT TGCCGAGAAC 
GGTAAAAGCA TCCATGAGCT AGAGAAATCC AGAAAACAGA TGGAGCTGGA GAAGGCTGAC 
ATCCAGATGG CGCTGGAGGA AGCAGAGGCA GCTCTTGAGC ACGAAGAAGC CAAGATTCTC 
CGGATCCAGC TTGAACTGAC CCAGGTGAAA TCAGAGATTG ATAGGAAGAT TGCAGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGGA CAGTGGAGAC CATGCAAGGC 
GCCCTGGACG CTGAGGTGCG CAGCAGGAAT GAGGCCATCC GCCTGAAGAA GAAGATGGAG 
GGCGACCTCA ATGAGATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC CGCAGAGACC 
ATCAAGCACC TCCGGAGCGT CCAGGGACAG CTGAAGGACA CCCAGCTGCA CCTGGATGAC 
GCTCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCCA TCGTGGAACG CAGAGCCAAC 
CTCCTGCAGG CTGAGGTGGA GGAGCTGCGG GCCACGCTGG AGCAGACGGA GAGGGCCCGG 
AAGCTGGCAG AGCAGGAGCT GTTGGATTCG AATGAGAGGG TCCAGCTGCT GCACACCCAG 
AACACAAGCC TCATCCACAC CAAGAAGAAG TTGGAGACTG ACCTCACGCA GCTCCAGAGT 
GAGGTGGAGG ATGCCTGCAG GGACGCAAGG AATGCAGAGG AGAAGGCCAA AAAGGCCATC 
ACTGACGCCG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTG 
GAGAGGATGA AGAAGAACCT GGAGCAGACG GTGAAGGACC TGCAGCACCG TCTGGATGAG 
GCTGAGCAGC TGGCGCTGAA GGGCGGCAAG AAGCAGATTC AGAAACTGGA GACACGGATC 
AGAGAGCTGG AGTTTGAGCT GGAAGGGGAG CAGAAGAGGA ACACAGAGTC TGTGAAGGGC 
CTGAGGAAGT ATGAGCGGCG TGTTAAGGAG CTCACATATC AGAGTGAGGA GGACAGGAAG 
AATGTGTTGA GATTGCAGGA TCTGGTGGAT AAACTCCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCTG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAGTT CAGGAAAGCC 
CAGCATGAGC TAGAGGAGGC CGAGGAACGG GCTGATATCG CAGAATCGCA AGTCAATAAA 
CTGCGGGCAA AGACCCGTGA CTTCACCTCT AGCCGGATGG TGGTCCATGA AAGCGAGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093105.1
CDS	45..5867
Translation	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYVKGKIKSSQDGKVTVETEDSRTLVVKPEDVYAMNPPKFDKIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVDGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGKAEAHFSLVHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATTDADGGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDERLAKLITRTQAVCRGFLMRVEFQKMMQRRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELAGLDETIAKLTREKKALQEAHQQTLDDLQAEEDKVNSLSKLKSKLEQQVDDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYSQLQSKVEDEQTLSLQLQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEATVATLRKKHADSAAELAEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSVESVSKSKANLEKICRTLEDQLSEARGKNEEMQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQIEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEAALKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKSIHELEKSRKQMELEKADIQMALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQGALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETIKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLTQLQSEVEDACRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKRNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	NM_001099635.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099635.1

ORF Insert Sequence:

ATGAGTAGCG ACACCGAGAT GGAAGTGTTT GGCATAGCTG CACCTTTCCT CCGCAAGTCG 
GAAAAGGAGA GGATCGAAGC TCAGAACCAG CCCTTTGATG CCAAAACCTA CTGCTTTGTG 
GTTGACTCAA AGGAAGAATA TGTCAAGGGG AAAATTAAGA GTAGCCAGGA TGGGAAAGTC 
ACTGTGGAGA CAGAAGACAG CAGGACCCTG GTGGTTAAGC CAGAGGACGT GTATGCCATG 
AACCCACCCA AGTTTGACAA GATTGAGGAC ATGGCCATGC TGACCCACCT GAACGAGCCC 
GCTGTGCTGT ACAACCTCAA GGACCGATAC ACGTCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAA TGGCTGCCAG TGTACAACCC CGAGGTGGTG 
GACGGCTACC GAGGCAAAAA ACGCCAGGAG GCTCCGCCCC ACATCTTCTC CATCTCCGAC 
AACGCCTACC AGTTCATGCT GACTGATCGT GAGAACCAGT CTATCCTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACGGT GAACACCAAG CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCCACTGGGG ACCTTGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACGCT GGAGGATCAA 
ATTATCAGCG CTAACCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACCGT GAGGAACGAC 
AACTCGTCTC GCTTTGGCAA GTTCATCCGC ATCCATTTTG GCACCACTGG GAAGCTGGCT 
TCTGCAGATA TTGAAACTTA TCTGCTGGAA AAGTCCAGAG TCACCTTCCA GCTGAAGGCT 
GAGAGAAGCT ACCACATCTT CTACCAGATT CTCTCCAACA AGAAACCTGA GCTGATTGAG 
CTGCTGCTGA TTACGACCAA CCCTTATGAC TACCCGTTCA TCAGCCAGGG CGAAATCCTG 
GTGGCCAGCA TCGACGATGC GGAGGAGCTG TTAGCTACGG ATAGTGCCAT CGACATCCTG 
GGCTTCACCC CGGAGGAGAA ATCTGGACTT TATAAACTGA CAGGGGCTGT GATGCATTAT 
GGGAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CCGAGCCAGA TGGCACTGAA 
GTGGCTGACA AAACAGCCTA CTTGATGGGC CTGAATTCCT CAGACCTCCT CAAGGCCTTG 
TGCTTTCCCA GAGTGAAAGT CGGGAATGAA TACGTTACAA AAGGGCAGAC TGTGGACCAG 
GTTCACCATG CTGTGAACGC TCTCTCCAAG TCCGTGTACG AGAAGCTGTT CTTGTGGATG 
GTCACTCGTA TTAACCAGCA GCTGGATACA AAGCTACCAA GACAGCACTT CATTGGCGTT 
TTGGACATTG CGGGTTTTGA AATTTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAACAGGAG 
GAATACAAGA AGGAGGGCAT CGAGTGGACC TTCATCGACT TCGGGATGGA CCTGGCAGCC 
TGCATCGAGC TCATCGAGAA GCCGATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CGACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA CCTGGGAAAG 
TCCAACAACT TCCAGAAACC TAAGGTGGTC AAAGGCAAGG CTGAAGCCCA CTTCTCCCTG 
GTGCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGCT GGCTGGAGAA GAACAAGGAC 
CCCCTGAATG AGACCGTGGT GGGACTGTAC CAGAAGTCCT CCAACAGACT CCTGGCACAT 
CTCTATGCCA CCTTCGCTAC AACAGATGCG GACGGTGGGA AGAAGAAAGT CGCTAAGAAG 
AAAGGCTCTT CCTTCCAAAC CGTCTCTGCA CTGTTCAGGG AAAACTTGAA CAAGCTGATG 
TCCAATTTAA GAACCACACA CCCACACTTT GTACGCTGTA TAATACCCAA CGAAACCAAG 
ACCCCAGGGG CCATGGAACA CAGCCTTGTC CTGCACCAGC TCAGGTGTAA CGGGGTGCTG 
GAAGGCATCC GGATCTGCAG GAAGGGCTTC CCCAACAGGA TCCTGTATGG AGACTTTAAA 
CAGAGATACC GAGTGCTGAA CGCCAGTGCC ATCCCAGAAG GCCAGTTCAT TGACAGCAAG 
AAAGCTTGCG AGAAACTCTT GGCTTCCATT GACATCGACC ACACACAGTA CAAATTTGGA 
CACACCAAGG TGTTCTTTAA GGCTGGCTTG CTAGGAACCC TGGAAGAGAT GCGGGATGAG 
CGTTTGGCCA AACTGATCAC TCGGACACAA GCCGTGTGCA GAGGGTTTCT CATGCGTGTG 
GAATTCCAGA AGATGATGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATCCGT 
GCCTTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTG 
CTGAAGAGCG CGGAGACTGA GAAGGAGATG GCCACCATGA AGGAGGAGTT CCAGAAAACC 
AAGGATGAGC TGGCTAAGTC GGAGGCAAAG AGGAAGGAGC TAGAGGAGAA GCTCGTCACT 
TTGGTACAGG AGAAGAATGA CCTGCAACTT CAAGTACAGG CTGAGAGTGA AAATTTGTTG 
GATGCTGAGG AACGATGCGA CCAGCTGATC AAAGCCAAGT TCCAGCTGGA AGCCAAAATC 
AAAGAGGTGA CAGAGAGAGC CGAGGACGAG GAGGAGATCA ACGCGGAGCT CACGGCCAAG 
AAGAGGAAGC TGGAGGACGA GTGCTCGGAG CTGAAGAAGG ACATCGATGA CCTTGAGCTG 
ACACTGGCCA AGGTTGAGAA GGAGAAGCAC GCCACAGAGA ACAAGGTCAA AAACCTTACC 
GAAGAACTGG CAGGCCTGGA TGAAACTATT GCAAAGCTAA CACGGGAGAA GAAGGCCCTC 
CAAGAGGCCC ACCAGCAGAC CCTGGACGAC CTCCAGGCTG AAGAAGACAA GGTCAATTCT 
CTGAGCAAGC TCAAGAGCAA GCTGGAGCAG CAAGTGGATG ATCTGGAGAG CTCCCTCGAA 
CAAGAAAAGA AGCTGCGTGT GGACCTAGAA AGGAACAAAA GGAAGCTGGA GGGAGACCTG 
AAGCTTGCCC AAGAGTCCAT ATTAGATCTG GAGAACGACA AGCAACAGCT AGACGAGAGG 
CTCAAGAAGA AGGACTTTGA ATATAGTCAG CTGCAAAGCA AAGTGGAAGA TGAGCAGACC 
TTGAGCCTCC AGCTTCAGAA GAAAATCAAG GAGCTACAGG CTCGCATCGA GGAGCTGGAG 
GAAGAGATAG AGGCAGAGAG GGCCACCCGG GCCAAGACAG AGAAGCAGCG CAGCGACTAT 
GCACGCGAGC TGGAGGAGCT GAGTGAGCGG CTGGAGGAGG CGGGCGGTGT CACCTCCACC 
CAGATCGAAC TGAACAAGAA GCGTGAGGCT GAGTTCTTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACCC TGCAGCACGA GGCCACGGTG GCCACGCTGC GGAAGAAGCA CGCGGACAGC 
GCGGCCGAGC TGGCGGAGCA GATCGACAAC CTGCAGCGGG TGAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATTGAC GACCTCTCCA GCAGCGTAGA GAGCGTGTCC 
AAATCCAAGG CCAATCTGGA GAAGATCTGC CGAACTCTAG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAGAT GCAGAGGAGC CTAAGTGAGC TGACCACACA GAAGTCCCGG 
CTGCAGACAG AGGCGGGTGA GCTGAGTCGT CAACTAGAAG AAAAGGAAAG CATTGTATCT 
CAGCTCTCCA GGAGCAAGCA GGCCTTTACC CAACAGATAG AGGAGCTCAA GAGGCAACTG 
GAGGAAGAGA ACAAGGCCAA GAACGCGCTG GCCCACGCCC TGCAGTCCTC CCGCCATGAC 
TGTGACCTGC TGCGGGAACA GTACGAGGAG GAGCAGGAAG GCAAAGCCGA GCTGCAGAGG 
GCACTGTCCA AGGCCAACAG CGAGGTGGCC CAGTGGAGGA CCAAGTATGA AACGGATGCC 
ATCCAGCGCA CGGAGGAGCT GGAGGAGGCC AAAAAAAAGC TTGCGCAGCG CCTTCAGGAT 
TCGGAGGAGC AGGTAGAGGC TGTAAATGCC AAATGTGCTT CCTTGGAAAA GACCAAGCAG 
AGGCTGCAAG GGGAGGTGGA GGACCTGATG GTGGATGTGG AAAGAGCCAA CTCCCTGGCT 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGC TAGCTGAGTG GAAGACGAAG 
TGTGAGGAGA GCCAAGCCGA GCTGGAGGCA GCTCTCAAGG AATCCCGGTC CTTGAGCACT 
GAGCTCTTCA AACTGAAGAA TGCCTATGAA GAAGCCTTGG ATCAACTTGA AACCGTGAAA 
CGGGAGAATA AGAACTTAGA ACAAGAGATA GCAGATCTCA CTGAACAGAT TGCCGAGAAC 
GGTAAAAGCA TCCATGAGCT AGAGAAATCC AGAAAACAGA TGGAGCTGGA GAAGGCTGAC 
ATCCAGATGG CGCTGGAGGA AGCAGAGGCA GCTCTTGAGC ACGAAGAAGC CAAGATTCTC 
CGGATCCAGC TTGAACTGAC CCAGGTGAAA TCAGAGATTG ATAGGAAGAT TGCAGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGGA CAGTGGAGAC CATGCAAGGC 
GCCCTGGACG CTGAGGTGCG CAGCAGGAAT GAGGCCATCC GCCTGAAGAA GAAGATGGAG 
GGCGACCTCA ATGAGATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC CGCAGAGACC 
ATCAAGCACC TCCGGAGCGT CCAGGGACAG CTGAAGGACA CCCAGCTGCA CCTGGATGAC 
GCTCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCCA TCGTGGAACG CAGAGCCAAC 
CTCCTGCAGG CTGAGGTGGA GGAGCTGCGG GCCACGCTGG AGCAGACGGA GAGGGCCCGG 
AAGCTGGCAG AGCAGGAGCT GTTGGATTCG AATGAGAGGG TCCAGCTGCT GCACACCCAG 
AACACAAGCC TCATCCACAC CAAGAAGAAG TTGGAGACTG ACCTCACGCA GCTCCAGAGT 
GAGGTGGAGG ATGCCTGCAG GGACGCAAGG AATGCAGAGG AGAAGGCCAA AAAGGCCATC 
ACTGACGCCG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTG 
GAGAGGATGA AGAAGAACCT GGAGCAGACG GTGAAGGACC TGCAGCACCG TCTGGATGAG 
GCTGAGCAGC TGGCGCTGAA GGGCGGCAAG AAGCAGATTC AGAAACTGGA GACACGGATC 
AGAGAGCTGG AGTTTGAGCT GGAAGGGGAG CAGAAGAGGA ACACAGAGTC TGTGAAGGGC 
CTGAGGAAGT ATGAGCGGCG TGTTAAGGAG CTCACATATC AGAGTGAGGA GGACAGGAAG 
AATGTGTTGA GATTGCAGGA TCTGGTGGAT AAACTCCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCTG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAGTT CAGGAAAGCC 
CAGCATGAGC TAGAGGAGGC CGAGGAACGG GCTGATATCG CAGAATCGCA AGTCAATAAA 
CTGCGGGCAA AGACCCGTGA CTTCACCTCT AGCCGGATGG TGGTCCATGA AAGCGAGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh3 ( XM_006532412.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532475.1 Mus musculus myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3), transcript variant X1, mRNA.

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CloneID	OMu20095
Clone ID Related Accession (Same CDS sequence)	NM_001099635.1 , XM_006532412.1
Accession Version	XM_006532412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	myosin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCG ACACCGAGAT GGAAGTGTTT GGCATAGCTG CACCTTTCCT CCGCAAGTCG 
GAAAAGGAGA GGATCGAAGC TCAGAACCAG CCCTTTGATG CCAAAACCTA CTGCTTTGTG 
GTTGACTCAA AGGAAGAATA TGTCAAGGGG AAAATTAAGA GTAGCCAGGA TGGGAAAGTC 
ACTGTGGAGA CAGAAGACAG CAGGACCCTG GTGGTTAAGC CAGAGGACGT GTATGCCATG 
AACCCACCCA AGTTTGACAA GATTGAGGAC ATGGCCATGC TGACCCACCT GAACGAGCCC 
GCTGTGCTGT ACAACCTCAA GGACCGATAC ACGTCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAA TGGCTGCCAG TGTACAACCC CGAGGTGGTG 
GACGGCTACC GAGGCAAAAA ACGCCAGGAG GCTCCGCCCC ACATCTTCTC CATCTCCGAC 
AACGCCTACC AGTTCATGCT GACTGATCGT GAGAACCAGT CTATCCTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACGGT GAACACCAAG CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCCACTGGGG ACCTTGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACGCT GGAGGATCAA 
ATTATCAGCG CTAACCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACCGT GAGGAACGAC 
AACTCGTCTC GCTTTGGCAA GTTCATCCGC ATCCATTTTG GCACCACTGG GAAGCTGGCT 
TCTGCAGATA TTGAAACTTA TCTGCTGGAA AAGTCCAGAG TCACCTTCCA GCTGAAGGCT 
GAGAGAAGCT ACCACATCTT CTACCAGATT CTCTCCAACA AGAAACCTGA GCTGATTGAG 
CTGCTGCTGA TTACGACCAA CCCTTATGAC TACCCGTTCA TCAGCCAGGG CGAAATCCTG 
GTGGCCAGCA TCGACGATGC GGAGGAGCTG TTAGCTACGG ATAGTGCCAT CGACATCCTG 
GGCTTCACCC CGGAGGAGAA ATCTGGACTT TATAAACTGA CAGGGGCTGT GATGCATTAT 
GGGAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CCGAGCCAGA TGGCACTGAA 
GTGGCTGACA AAACAGCCTA CTTGATGGGC CTGAATTCCT CAGACCTCCT CAAGGCCTTG 
TGCTTTCCCA GAGTGAAAGT CGGGAATGAA TACGTTACAA AAGGGCAGAC TGTGGACCAG 
GTTCACCATG CTGTGAACGC TCTCTCCAAG TCCGTGTACG AGAAGCTGTT CTTGTGGATG 
GTCACTCGTA TTAACCAGCA GCTGGATACA AAGCTACCAA GACAGCACTT CATTGGCGTT 
TTGGACATTG CGGGTTTTGA AATTTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAACAGGAG 
GAATACAAGA AGGAGGGCAT CGAGTGGACC TTCATCGACT TCGGGATGGA CCTGGCAGCC 
TGCATCGAGC TCATCGAGAA GCCGATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CGACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA CCTGGGAAAG 
TCCAACAACT TCCAGAAACC TAAGGTGGTC AAAGGCAAGG CTGAAGCCCA CTTCTCCCTG 
GTGCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGCT GGCTGGAGAA GAACAAGGAC 
CCCCTGAATG AGACCGTGGT GGGACTGTAC CAGAAGTCCT CCAACAGACT CCTGGCACAT 
CTCTATGCCA CCTTCGCTAC AACAGATGCG GACGGTGGGA AGAAGAAAGT CGCTAAGAAG 
AAAGGCTCTT CCTTCCAAAC CGTCTCTGCA CTGTTCAGGG AAAACTTGAA CAAGCTGATG 
TCCAATTTAA GAACCACACA CCCACACTTT GTACGCTGTA TAATACCCAA CGAAACCAAG 
ACCCCAGGGG CCATGGAACA CAGCCTTGTC CTGCACCAGC TCAGGTGTAA CGGGGTGCTG 
GAAGGCATCC GGATCTGCAG GAAGGGCTTC CCCAACAGGA TCCTGTATGG AGACTTTAAA 
CAGAGATACC GAGTGCTGAA CGCCAGTGCC ATCCCAGAAG GCCAGTTCAT TGACAGCAAG 
AAAGCTTGCG AGAAACTCTT GGCTTCCATT GACATCGACC ACACACAGTA CAAATTTGGA 
CACACCAAGG TGTTCTTTAA GGCTGGCTTG CTAGGAACCC TGGAAGAGAT GCGGGATGAG 
CGTTTGGCCA AACTGATCAC TCGGACACAA GCCGTGTGCA GAGGGTTTCT CATGCGTGTG 
GAATTCCAGA AGATGATGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATCCGT 
GCCTTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTG 
CTGAAGAGCG CGGAGACTGA GAAGGAGATG GCCACCATGA AGGAGGAGTT CCAGAAAACC 
AAGGATGAGC TGGCTAAGTC GGAGGCAAAG AGGAAGGAGC TAGAGGAGAA GCTCGTCACT 
TTGGTACAGG AGAAGAATGA CCTGCAACTT CAAGTACAGG CTGAGAGTGA AAATTTGTTG 
GATGCTGAGG AACGATGCGA CCAGCTGATC AAAGCCAAGT TCCAGCTGGA AGCCAAAATC 
AAAGAGGTGA CAGAGAGAGC CGAGGACGAG GAGGAGATCA ACGCGGAGCT CACGGCCAAG 
AAGAGGAAGC TGGAGGACGA GTGCTCGGAG CTGAAGAAGG ACATCGATGA CCTTGAGCTG 
ACACTGGCCA AGGTTGAGAA GGAGAAGCAC GCCACAGAGA ACAAGGTCAA AAACCTTACC 
GAAGAACTGG CAGGCCTGGA TGAAACTATT GCAAAGCTAA CACGGGAGAA GAAGGCCCTC 
CAAGAGGCCC ACCAGCAGAC CCTGGACGAC CTCCAGGCTG AAGAAGACAA GGTCAATTCT 
CTGAGCAAGC TCAAGAGCAA GCTGGAGCAG CAAGTGGATG ATCTGGAGAG CTCCCTCGAA 
CAAGAAAAGA AGCTGCGTGT GGACCTAGAA AGGAACAAAA GGAAGCTGGA GGGAGACCTG 
AAGCTTGCCC AAGAGTCCAT ATTAGATCTG GAGAACGACA AGCAACAGCT AGACGAGAGG 
CTCAAGAAGA AGGACTTTGA ATATAGTCAG CTGCAAAGCA AAGTGGAAGA TGAGCAGACC 
TTGAGCCTCC AGCTTCAGAA GAAAATCAAG GAGCTACAGG CTCGCATCGA GGAGCTGGAG 
GAAGAGATAG AGGCAGAGAG GGCCACCCGG GCCAAGACAG AGAAGCAGCG CAGCGACTAT 
GCACGCGAGC TGGAGGAGCT GAGTGAGCGG CTGGAGGAGG CGGGCGGTGT CACCTCCACC 
CAGATCGAAC TGAACAAGAA GCGTGAGGCT GAGTTCTTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACCC TGCAGCACGA GGCCACGGTG GCCACGCTGC GGAAGAAGCA CGCGGACAGC 
GCGGCCGAGC TGGCGGAGCA GATCGACAAC CTGCAGCGGG TGAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATTGAC GACCTCTCCA GCAGCGTAGA GAGCGTGTCC 
AAATCCAAGG CCAATCTGGA GAAGATCTGC CGAACTCTAG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAGAT GCAGAGGAGC CTAAGTGAGC TGACCACACA GAAGTCCCGG 
CTGCAGACAG AGGCGGGTGA GCTGAGTCGT CAACTAGAAG AAAAGGAAAG CATTGTATCT 
CAGCTCTCCA GGAGCAAGCA GGCCTTTACC CAACAGATAG AGGAGCTCAA GAGGCAACTG 
GAGGAAGAGA ACAAGGCCAA GAACGCGCTG GCCCACGCCC TGCAGTCCTC CCGCCATGAC 
TGTGACCTGC TGCGGGAACA GTACGAGGAG GAGCAGGAAG GCAAAGCCGA GCTGCAGAGG 
GCACTGTCCA AGGCCAACAG CGAGGTGGCC CAGTGGAGGA CCAAGTATGA AACGGATGCC 
ATCCAGCGCA CGGAGGAGCT GGAGGAGGCC AAAAAAAAGC TTGCGCAGCG CCTTCAGGAT 
TCGGAGGAGC AGGTAGAGGC TGTAAATGCC AAATGTGCTT CCTTGGAAAA GACCAAGCAG 
AGGCTGCAAG GGGAGGTGGA GGACCTGATG GTGGATGTGG AAAGAGCCAA CTCCCTGGCT 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGC TAGCTGAGTG GAAGACGAAG 
TGTGAGGAGA GCCAAGCCGA GCTGGAGGCA GCTCTCAAGG AATCCCGGTC CTTGAGCACT 
GAGCTCTTCA AACTGAAGAA TGCCTATGAA GAAGCCTTGG ATCAACTTGA AACCGTGAAA 
CGGGAGAATA AGAACTTAGA ACAAGAGATA GCAGATCTCA CTGAACAGAT TGCCGAGAAC 
GGTAAAAGCA TCCATGAGCT AGAGAAATCC AGAAAACAGA TGGAGCTGGA GAAGGCTGAC 
ATCCAGATGG CGCTGGAGGA AGCAGAGGCA GCTCTTGAGC ACGAAGAAGC CAAGATTCTC 
CGGATCCAGC TTGAACTGAC CCAGGTGAAA TCAGAGATTG ATAGGAAGAT TGCAGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGGA CAGTGGAGAC CATGCAAGGC 
GCCCTGGACG CTGAGGTGCG CAGCAGGAAT GAGGCCATCC GCCTGAAGAA GAAGATGGAG 
GGCGACCTCA ATGAGATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC CGCAGAGACC 
ATCAAGCACC TCCGGAGCGT CCAGGGACAG CTGAAGGACA CCCAGCTGCA CCTGGATGAC 
GCTCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCCA TCGTGGAACG CAGAGCCAAC 
CTCCTGCAGG CTGAGGTGGA GGAGCTGCGG GCCACGCTGG AGCAGACGGA GAGGGCCCGG 
AAGCTGGCAG AGCAGGAGCT GTTGGATTCG AATGAGAGGG TCCAGCTGCT GCACACCCAG 
AACACAAGCC TCATCCACAC CAAGAAGAAG TTGGAGACTG ACCTCACGCA GCTCCAGAGT 
GAGGTGGAGG ATGCCTGCAG GGACGCAAGG AATGCAGAGG AGAAGGCCAA AAAGGCCATC 
ACTGACGCCG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTG 
GAGAGGATGA AGAAGAACCT GGAGCAGACG GTGAAGGACC TGCAGCACCG TCTGGATGAG 
GCTGAGCAGC TGGCGCTGAA GGGCGGCAAG AAGCAGATTC AGAAACTGGA GACACGGATC 
AGAGAGCTGG AGTTTGAGCT GGAAGGGGAG CAGAAGAGGA ACACAGAGTC TGTGAAGGGC 
CTGAGGAAGT ATGAGCGGCG TGTTAAGGAG CTCACATATC AGAGTGAGGA GGACAGGAAG 
AATGTGTTGA GATTGCAGGA TCTGGTGGAT AAACTCCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCTG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAGTT CAGGAAAGCC 
CAGCATGAGC TAGAGGAGGC CGAGGAACGG GCTGATATCG CAGAATCGCA AGTCAATAAA 
CTGCGGGCAA AGACCCGTGA CTTCACCTCT AGCCGGATGG TGGTCCATGA AAGCGAGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532475.1
CDS	116..5938
Translation	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYVKGKIKSSQDGKVTVETEDSRTLVVKPEDVYAMNPPKFDKIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVDGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGKAEAHFSLVHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATTDADGGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDERLAKLITRTQAVCRGFLMRVEFQKMMQRRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELAGLDETIAKLTREKKALQEAHQQTLDDLQAEEDKVNSLSKLKSKLEQQVDDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYSQLQSKVEDEQTLSLQLQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEATVATLRKKHADSAAELAEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSVESVSKSKANLEKICRTLEDQLSEARGKNEEMQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQIEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEAALKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKSIHELEKSRKQMELEKADIQMALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQGALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETIKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLTQLQSEVEDACRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKRNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	XM_006532412.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532412.1

ORF Insert Sequence:

ATGAGTAGCG ACACCGAGAT GGAAGTGTTT GGCATAGCTG CACCTTTCCT CCGCAAGTCG 
GAAAAGGAGA GGATCGAAGC TCAGAACCAG CCCTTTGATG CCAAAACCTA CTGCTTTGTG 
GTTGACTCAA AGGAAGAATA TGTCAAGGGG AAAATTAAGA GTAGCCAGGA TGGGAAAGTC 
ACTGTGGAGA CAGAAGACAG CAGGACCCTG GTGGTTAAGC CAGAGGACGT GTATGCCATG 
AACCCACCCA AGTTTGACAA GATTGAGGAC ATGGCCATGC TGACCCACCT GAACGAGCCC 
GCTGTGCTGT ACAACCTCAA GGACCGATAC ACGTCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAA TGGCTGCCAG TGTACAACCC CGAGGTGGTG 
GACGGCTACC GAGGCAAAAA ACGCCAGGAG GCTCCGCCCC ACATCTTCTC CATCTCCGAC 
AACGCCTACC AGTTCATGCT GACTGATCGT GAGAACCAGT CTATCCTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACGGT GAACACCAAG CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCCACTGGGG ACCTTGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACGCT GGAGGATCAA 
ATTATCAGCG CTAACCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACCGT GAGGAACGAC 
AACTCGTCTC GCTTTGGCAA GTTCATCCGC ATCCATTTTG GCACCACTGG GAAGCTGGCT 
TCTGCAGATA TTGAAACTTA TCTGCTGGAA AAGTCCAGAG TCACCTTCCA GCTGAAGGCT 
GAGAGAAGCT ACCACATCTT CTACCAGATT CTCTCCAACA AGAAACCTGA GCTGATTGAG 
CTGCTGCTGA TTACGACCAA CCCTTATGAC TACCCGTTCA TCAGCCAGGG CGAAATCCTG 
GTGGCCAGCA TCGACGATGC GGAGGAGCTG TTAGCTACGG ATAGTGCCAT CGACATCCTG 
GGCTTCACCC CGGAGGAGAA ATCTGGACTT TATAAACTGA CAGGGGCTGT GATGCATTAT 
GGGAACATGA AGTTCAAGCA GAAGCAGCGG GAGGAGCAGG CCGAGCCAGA TGGCACTGAA 
GTGGCTGACA AAACAGCCTA CTTGATGGGC CTGAATTCCT CAGACCTCCT CAAGGCCTTG 
TGCTTTCCCA GAGTGAAAGT CGGGAATGAA TACGTTACAA AAGGGCAGAC TGTGGACCAG 
GTTCACCATG CTGTGAACGC TCTCTCCAAG TCCGTGTACG AGAAGCTGTT CTTGTGGATG 
GTCACTCGTA TTAACCAGCA GCTGGATACA AAGCTACCAA GACAGCACTT CATTGGCGTT 
TTGGACATTG CGGGTTTTGA AATTTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAACG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAACAGGAG 
GAATACAAGA AGGAGGGCAT CGAGTGGACC TTCATCGACT TCGGGATGGA CCTGGCAGCC 
TGCATCGAGC TCATCGAGAA GCCGATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CGACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA CCTGGGAAAG 
TCCAACAACT TCCAGAAACC TAAGGTGGTC AAAGGCAAGG CTGAAGCCCA CTTCTCCCTG 
GTGCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGCT GGCTGGAGAA GAACAAGGAC 
CCCCTGAATG AGACCGTGGT GGGACTGTAC CAGAAGTCCT CCAACAGACT CCTGGCACAT 
CTCTATGCCA CCTTCGCTAC AACAGATGCG GACGGTGGGA AGAAGAAAGT CGCTAAGAAG 
AAAGGCTCTT CCTTCCAAAC CGTCTCTGCA CTGTTCAGGG AAAACTTGAA CAAGCTGATG 
TCCAATTTAA GAACCACACA CCCACACTTT GTACGCTGTA TAATACCCAA CGAAACCAAG 
ACCCCAGGGG CCATGGAACA CAGCCTTGTC CTGCACCAGC TCAGGTGTAA CGGGGTGCTG 
GAAGGCATCC GGATCTGCAG GAAGGGCTTC CCCAACAGGA TCCTGTATGG AGACTTTAAA 
CAGAGATACC GAGTGCTGAA CGCCAGTGCC ATCCCAGAAG GCCAGTTCAT TGACAGCAAG 
AAAGCTTGCG AGAAACTCTT GGCTTCCATT GACATCGACC ACACACAGTA CAAATTTGGA 
CACACCAAGG TGTTCTTTAA GGCTGGCTTG CTAGGAACCC TGGAAGAGAT GCGGGATGAG 
CGTTTGGCCA AACTGATCAC TCGGACACAA GCCGTGTGCA GAGGGTTTCT CATGCGTGTG 
GAATTCCAGA AGATGATGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATCCGT 
GCCTTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTG 
CTGAAGAGCG CGGAGACTGA GAAGGAGATG GCCACCATGA AGGAGGAGTT CCAGAAAACC 
AAGGATGAGC TGGCTAAGTC GGAGGCAAAG AGGAAGGAGC TAGAGGAGAA GCTCGTCACT 
TTGGTACAGG AGAAGAATGA CCTGCAACTT CAAGTACAGG CTGAGAGTGA AAATTTGTTG 
GATGCTGAGG AACGATGCGA CCAGCTGATC AAAGCCAAGT TCCAGCTGGA AGCCAAAATC 
AAAGAGGTGA CAGAGAGAGC CGAGGACGAG GAGGAGATCA ACGCGGAGCT CACGGCCAAG 
AAGAGGAAGC TGGAGGACGA GTGCTCGGAG CTGAAGAAGG ACATCGATGA CCTTGAGCTG 
ACACTGGCCA AGGTTGAGAA GGAGAAGCAC GCCACAGAGA ACAAGGTCAA AAACCTTACC 
GAAGAACTGG CAGGCCTGGA TGAAACTATT GCAAAGCTAA CACGGGAGAA GAAGGCCCTC 
CAAGAGGCCC ACCAGCAGAC CCTGGACGAC CTCCAGGCTG AAGAAGACAA GGTCAATTCT 
CTGAGCAAGC TCAAGAGCAA GCTGGAGCAG CAAGTGGATG ATCTGGAGAG CTCCCTCGAA 
CAAGAAAAGA AGCTGCGTGT GGACCTAGAA AGGAACAAAA GGAAGCTGGA GGGAGACCTG 
AAGCTTGCCC AAGAGTCCAT ATTAGATCTG GAGAACGACA AGCAACAGCT AGACGAGAGG 
CTCAAGAAGA AGGACTTTGA ATATAGTCAG CTGCAAAGCA AAGTGGAAGA TGAGCAGACC 
TTGAGCCTCC AGCTTCAGAA GAAAATCAAG GAGCTACAGG CTCGCATCGA GGAGCTGGAG 
GAAGAGATAG AGGCAGAGAG GGCCACCCGG GCCAAGACAG AGAAGCAGCG CAGCGACTAT 
GCACGCGAGC TGGAGGAGCT GAGTGAGCGG CTGGAGGAGG CGGGCGGTGT CACCTCCACC 
CAGATCGAAC TGAACAAGAA GCGTGAGGCT GAGTTCTTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACCC TGCAGCACGA GGCCACGGTG GCCACGCTGC GGAAGAAGCA CGCGGACAGC 
GCGGCCGAGC TGGCGGAGCA GATCGACAAC CTGCAGCGGG TGAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATTGAC GACCTCTCCA GCAGCGTAGA GAGCGTGTCC 
AAATCCAAGG CCAATCTGGA GAAGATCTGC CGAACTCTAG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAGAT GCAGAGGAGC CTAAGTGAGC TGACCACACA GAAGTCCCGG 
CTGCAGACAG AGGCGGGTGA GCTGAGTCGT CAACTAGAAG AAAAGGAAAG CATTGTATCT 
CAGCTCTCCA GGAGCAAGCA GGCCTTTACC CAACAGATAG AGGAGCTCAA GAGGCAACTG 
GAGGAAGAGA ACAAGGCCAA GAACGCGCTG GCCCACGCCC TGCAGTCCTC CCGCCATGAC 
TGTGACCTGC TGCGGGAACA GTACGAGGAG GAGCAGGAAG GCAAAGCCGA GCTGCAGAGG 
GCACTGTCCA AGGCCAACAG CGAGGTGGCC CAGTGGAGGA CCAAGTATGA AACGGATGCC 
ATCCAGCGCA CGGAGGAGCT GGAGGAGGCC AAAAAAAAGC TTGCGCAGCG CCTTCAGGAT 
TCGGAGGAGC AGGTAGAGGC TGTAAATGCC AAATGTGCTT CCTTGGAAAA GACCAAGCAG 
AGGCTGCAAG GGGAGGTGGA GGACCTGATG GTGGATGTGG AAAGAGCCAA CTCCCTGGCT 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGC TAGCTGAGTG GAAGACGAAG 
TGTGAGGAGA GCCAAGCCGA GCTGGAGGCA GCTCTCAAGG AATCCCGGTC CTTGAGCACT 
GAGCTCTTCA AACTGAAGAA TGCCTATGAA GAAGCCTTGG ATCAACTTGA AACCGTGAAA 
CGGGAGAATA AGAACTTAGA ACAAGAGATA GCAGATCTCA CTGAACAGAT TGCCGAGAAC 
GGTAAAAGCA TCCATGAGCT AGAGAAATCC AGAAAACAGA TGGAGCTGGA GAAGGCTGAC 
ATCCAGATGG CGCTGGAGGA AGCAGAGGCA GCTCTTGAGC ACGAAGAAGC CAAGATTCTC 
CGGATCCAGC TTGAACTGAC CCAGGTGAAA TCAGAGATTG ATAGGAAGAT TGCAGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGGA CAGTGGAGAC CATGCAAGGC 
GCCCTGGACG CTGAGGTGCG CAGCAGGAAT GAGGCCATCC GCCTGAAGAA GAAGATGGAG 
GGCGACCTCA ATGAGATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC CGCAGAGACC 
ATCAAGCACC TCCGGAGCGT CCAGGGACAG CTGAAGGACA CCCAGCTGCA CCTGGATGAC 
GCTCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCCA TCGTGGAACG CAGAGCCAAC 
CTCCTGCAGG CTGAGGTGGA GGAGCTGCGG GCCACGCTGG AGCAGACGGA GAGGGCCCGG 
AAGCTGGCAG AGCAGGAGCT GTTGGATTCG AATGAGAGGG TCCAGCTGCT GCACACCCAG 
AACACAAGCC TCATCCACAC CAAGAAGAAG TTGGAGACTG ACCTCACGCA GCTCCAGAGT 
GAGGTGGAGG ATGCCTGCAG GGACGCAAGG AATGCAGAGG AGAAGGCCAA AAAGGCCATC 
ACTGACGCCG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTG 
GAGAGGATGA AGAAGAACCT GGAGCAGACG GTGAAGGACC TGCAGCACCG TCTGGATGAG 
GCTGAGCAGC TGGCGCTGAA GGGCGGCAAG AAGCAGATTC AGAAACTGGA GACACGGATC 
AGAGAGCTGG AGTTTGAGCT GGAAGGGGAG CAGAAGAGGA ACACAGAGTC TGTGAAGGGC 
CTGAGGAAGT ATGAGCGGCG TGTTAAGGAG CTCACATATC AGAGTGAGGA GGACAGGAAG 
AATGTGTTGA GATTGCAGGA TCTGGTGGAT AAACTCCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCTG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAGTT CAGGAAAGCC 
CAGCATGAGC TAGAGGAGGC CGAGGAACGG GCTGATATCG CAGAATCGCA AGTCAATAAA 
CTGCGGGCAA AGACCCGTGA CTTCACCTCT AGCCGGATGG TGGTCCATGA AAGCGAGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration.
Nature communications10(1)1796(2019 04)
Sala D,Cunningham TJ,Stec MJ,Etxaniz U,Nicoletti C,Dall'Agnese A,Puri PL,Duester G,Latella L,Sacco A

Deletion of the T-box transcription factor gene, Tbx1, in mice induces differential expression of genes associated with cleft palate in humans.
Archives of oral biology95149-155(2018 Nov)
Funato N,Yanagisawa H

ANKK1 is found in myogenic precursors and muscle fibers subtypes with glycolytic metabolism.
PloS one13(5)e0197254(2018)
Rubio-Solsona E,Martí S,Vílchez JJ,Palau F,Hoenicka J

Clathrin adaptor GGA1 modulates myogenesis of C2C12 myoblasts.
PloS one13(11)e0207533(2018)
Isobe M,Lee S,Waguri S,Kametaka S

HOXA5 plays tissue-specific roles in the developing respiratory system.
Development (Cambridge, England)144(19)3547-3561(2017 10)
Landry-Truchon K,Houde N,Boucherat O,Joncas FH,Dasen JS,Philippidou P,Mansfield JH,Jeannotte L

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Myh3 cDNA ORF clone, Mus musculus(house mouse)

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