Smad1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smad1
Entrez Gene ID	17125
Full Name	SMAD family member 1
Synonyms	AI528653,Madh1,Madr1,Mlp1,MusMLP
General protein information	Preferred Names SMAD family member 1 Names mothers against decapentaplegic homolog 1 MAD homolog 1 Smad 1 dwarfin-A dwf-A mMad1 mad-related protein 1 mothers against DPP homolog 1 mothers-against-DPP-related 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530746.2	XP_006530809.1	mothers against decapentaplegic homolog 1 isoform X1
XM_006530746.3	XP_006530809.1	mothers against decapentaplegic homolog 1 isoform X1
NM_008539.3	NP_032565.2	mothers against decapentaplegic homolog 1
NM_008539.4	NP_032565.2	mothers against decapentaplegic homolog 1
XM_006530746.4	XP_006530809.1	mothers against decapentaplegic homolog 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMAD1	XP_001148403.1
Xenopus tropicalis (tropical clawed frog)	smad1	NP_001007481.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008551	XP_314661.3
Homo sapiens (human)	SMAD1	NP_001003688.1
Bos taurus (cattle)	SMAD1	NP_001069691.2
Gallus gallus (chicken)	SMAD1	NP_001188384.1
Canis lupus familiaris (dog)	SMAD1	XP_005629368.1
Rattus norvegicus (Norway rat)	Smad1	NP_037262.2
Macaca mulatta (Rhesus monkey)	SMAD1	XP_001096679.1
Mus musculus (house mouse)	Smad1	NP_032565.2
Danio rerio (zebrafish)	smad1	NP_571431.1
Drosophila melanogaster (fruit fly)	Mad	NP_477017.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiple steps characterise ventricular layer attrition to form the ependymal cell lining of the adult mouse spinal cord central canal.
Cañizares MA, Albors AR, Singer G, Suttie N, Gorkic M, Felts P, Storey KG
Journal of anatomy236(2)334-350(2020 Feb)

Early dorsomedial tissue interactions regulate gyrification of distal neocortex.
Chizhikov VV, Iskusnykh IY, Steshina EY, Fattakhov N, Lindgren AG, Shetty AS, Roy A, Tole S, Millen KJ
Nature communications10(1)5192(2019 Nov)

The TGFβ type I receptor TGFβRI functions as an inhibitor of BMP signaling in cartilage.
Wang W, Chun H, Baek J, Sadik JE, Shirazyan A, Razavi P, Lopez N, Lyons KM
Proceedings of the National Academy of Sciences of the United States of America116(31)15570-15579(2019 Jul)

Loss of the small GTPase Arl8b results in abnormal development of the roof plate in mouse embryos.
Hashimoto K, Yamaguchi Y, Kishi Y, Kikko Y, Takasaki K, Maeda Y, Matsumoto Y, Oka M, Miura M, Ohata S, Katada T, Kontani K
Genes to cells : devoted to molecular & cellular mechanisms24(6)436-448(2019 Jun)

Effect of stimulated erythropoiesis on liver SMAD signaling pathway in iron-overloaded and iron-deficient mice.
Frýdlová J, Rogalsky DW, Truksa J, Nečas E, Vokurka M, Krijt J
PloS one14(4)e0215028(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smad1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smad1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20039	NM_008539.4 Latest version!	Mus musculus SMAD family member 1 (Smad1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OMu20039	XM_006530746.4 Latest version!	Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OMu20039	XM_006530746.2	Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OMu20039	NM_008539.3	Mus musculus SMAD family member 1 (Smad1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OMu20039	XM_006530746.3	Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smad1 ( NM_008539.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032565.2 Mus musculus SMAD family member 1 (Smad1), mRNA.

Price & Availability↑

CloneID	OMu20039
Clone ID Related Accession (Same CDS sequence)	XM_006530746.2 , XM_006530746.3 , NM_008539.3 , NM_008539.4 , XM_006530746.4
Accession Version	NM_008539.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514390400000
Organism	Mus musculus(house mouse)
Product	mothers against decapentaplegic homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC058693.1. On Feb 9, 2004 this sequence version replaced NM_008539.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058693.1, AK054104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032565.2
CDS	398..1795
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGGSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMMAPPLPAEISRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	NM_008539.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD family member 1 (Smad1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008539.3

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( XM_006530746.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530809.1 Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20039
Clone ID Related Accession (Same CDS sequence)	XM_006530746.2 , XM_006530746.3 , NM_008539.3 , NM_008539.4 , XM_006530746.4
Accession Version	XM_006530746.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423324800000
Organism	Mus musculus(House mouse)
Product	mothers against decapentaplegic homolog 1 isoform X1
Comment

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006530809.1

Translation

MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGGSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMMAPPLPAEISRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	XM_006530746.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530746.2

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( NM_008539.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032565.2 Mus musculus SMAD family member 1 (Smad1), mRNA.

Price & Availability↑

CloneID	OMu20039
Clone ID Related Accession (Same CDS sequence)	XM_006530746.2 , XM_006530746.3 , NM_008539.3 , NM_008539.4 , XM_006530746.4
Accession Version	NM_008539.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	mothers against decapentaplegic homolog 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC058693.1. On Jun 9, 2018 this sequence version replaced NM_008539.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058693.1, AK054104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032565.2
CDS	358..1755
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGGSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMMAPPLPAEISRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	NM_008539.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD family member 1 (Smad1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008539.4

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( XM_006530746.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530809.1 Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

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CloneID	OMu20039
Clone ID Related Accession (Same CDS sequence)	XM_006530746.2 , XM_006530746.3 , NM_008539.3 , NM_008539.4 , XM_006530746.4
Accession Version	XM_006530746.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	mothers against decapentaplegic homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530809.1
CDS	406..1803
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGGSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMMAPPLPAEISRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	XM_006530746.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530746.3

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( XM_006530746.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530809.1 Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20039
Clone ID Related Accession (Same CDS sequence)	XM_006530746.2 , XM_006530746.3 , NM_008539.3 , NM_008539.4 , XM_006530746.4
Accession Version	XM_006530746.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	mothers against decapentaplegic homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530746.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530809.1
CDS	403..1800
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGGSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMMAPPLPAEISRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	XM_006530746.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530746.4

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGTCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ATGAAGAAGA GAAATGGGCA GAGAAAGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCTGGACAG 
CCGAGTAACT GCGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTAC CTCATGTCAT TTATTGCCGT GTGTGGCGCT GGCCCGACCT CCAGAGCCAC 
CATGAACTGA AGCCTCTGGA ATGCTGTGAG TTCCCATTTG GTTCCAAGCA GAAGGAGGTC 
TGCATCAACC CCTACCACTA TAAGCGAGTG GAGAGCCCGG TTCTCCCGCC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA TCCTCAGCAC AGCCTTCTGG CTCAGTTCCG CAACCTGGGA 
CAAAATGAGC CTCACATGCC ACTGAACGCC ACGTTCCCAG ACTCTTTCCA GCAGCCCAAC 
AGCCACCCGT TCCCCCACTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CGGCAGCAGC 
AGCACCTACC CTCACTCCCC AACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCT 
GACACACCCC CACCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCCATGGACA CGAACATGAT GGCGCCTCCA CTGCCCGCTG AAATCAGCAG AGGAGATGTT 
CAGGCAGTTG CTTACGAGGA ACCAAAACAC TGGTGCTCTA TTGTGTACTA TGAGCTCAAC 
AACCGTGTGG GTGAAGCGTT CCACGCCTCG TCCACCAGCG TGCTGGTGGA TGGTTTCACA 
GATCCGTCCA ACAATAAGAA CCGCTTCTGC CTTGGCTTGC TCTCCAACGT TAACCGGAAT 
TCCACTATTG AAAACACCAG GCGACATATT GGGAAAGGAG TCCACCTTTA TTACGTTGGA 
GGAGAGGTGT ATGCGGAATG CCTCAGTGAC AGCAGCATCT TCGTGCAGAG CCGGAACTGC 
AACTACCACC ACGGCTTTCA CCCCACCACC GTCTGCAAGA TCCCCAGCGG GTGCAGCTTG 
AAAATCTTCA ACAACCAAGA GTTTGCTCAG CTACTGGCGC AGTCTGTGAA CCACGGGTTC 
GAGACCGTGT ATGAACTCAC CAAAATGTGC ACTATTCGGA TGAGCTTCGT GAAGGGTTGG 
GGAGCCGAAT ACCACCGGCA GGATGTTACC AGCACCCCCT GCTGGATTGA GATCCATCTG 
CATGGCCCTC TCCAGTGGCT GGATAAGGTT CTGACCCAGA TGGGCTCACC CCACAATCCT 
ATTTCATCCG TGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiple steps characterise ventricular layer attrition to form the ependymal cell lining of the adult mouse spinal cord central canal.
Journal of anatomy236(2)334-350(2020 Feb)
Cañizares MA,Albors AR,Singer G,Suttie N,Gorkic M,Felts P,Storey KG

Early dorsomedial tissue interactions regulate gyrification of distal neocortex.
Nature communications10(1)5192(2019 Nov)
Chizhikov VV,Iskusnykh IY,Steshina EY,Fattakhov N,Lindgren AG,Shetty AS,Roy A,Tole S,Millen KJ

The TGFβ type I receptor TGFβRI functions as an inhibitor of BMP signaling in cartilage.
Proceedings of the National Academy of Sciences of the United States of America116(31)15570-15579(2019 Jul)
Wang W,Chun H,Baek J,Sadik JE,Shirazyan A,Razavi P,Lopez N,Lyons KM

Loss of the small GTPase Arl8b results in abnormal development of the roof plate in mouse embryos.
Genes to cells : devoted to molecular & cellular mechanisms24(6)436-448(2019 Jun)
Hashimoto K,Yamaguchi Y,Kishi Y,Kikko Y,Takasaki K,Maeda Y,Matsumoto Y,Oka M,Miura M,Ohata S,Katada T,Kontani K

Effect of stimulated erythropoiesis on liver SMAD signaling pathway in iron-overloaded and iron-deficient mice.
PloS one14(4)e0215028(2019)
Frýdlová J,Rogalsky DW,Truksa J,Nečas E,Vokurka M,Krijt J

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Smad1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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