Itch cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itch
Entrez Gene ID	16396
Full Name	itchy, E3 ubiquitin protein ligase
Synonyms	6720481N21Rik,8030492O04Rik,A130065M08,AIP4,C230047C07Rik
General protein information	Preferred Names itchy, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase Itchy HECT-type E3 ubiquitin transferase Itchy homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H1\|2 76.94 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008395.3	NP_032421.2	E3 ubiquitin-protein ligase Itchy
NM_001243712.1	NP_001230641.1	E3 ubiquitin-protein ligase Itchy

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITCH	NP_113671.3
Canis lupus familiaris (dog)	ITCH	XP_851460.1
Mus musculus (house mouse)	Itch	NP_001230641.1
Rattus norvegicus (Norway rat)	Itch	NP_001005887.1
Bos taurus (cattle)	ITCH	NP_001075897.1
Gallus gallus (chicken)	ITCH	XP_417330.2
Danio rerio (zebrafish)	LOC100330031	XP_003201702.2
Danio rerio (zebrafish)	itch	XP_002667578.2
Macaca mulatta (Rhesus monkey)	LOC707640	XP_002808005.1
Xenopus tropicalis (tropical clawed frog)	itch	NP_001072737.1

Gene Ontology
Bibliography

Related articles in PubMed

The E3 ubiquitin ligase Itch restricts antigen-driven B cell responses.
Moser EK, Roof J, Dybas JM, Spruce LA, Seeholzer SH, Cancro MP, Oliver PM
The Journal of experimental medicine216(9)2170-2183(2019 Sep)

Notch Activation by Shootin1 Opposing Activities on 2 Ubiquitin Ligases.
Sapir T, Levy T, Kozer N, Shin I, Zamor V, Haffner-Krausz R, McGlade JC, Reiner O
Cerebral cortex (New York, N.Y. : 1991)28(9)3115-3128(2018 09)

The E3 ligases Itch and WWP2 cooperate to limit T
Aki D, Li H, Zhang W, Zheng M, Elly C, Lee JH, Zou W, Liu YC
Nature immunology19(7)766-775(2018 07)

Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis.
Infante P, Faedda R, Bernardi F, Bufalieri F, Lospinoso Severini L, Alfonsi R, Mazzà D, Siler M, Coni S, Po A, Petroni M, Ferretti E, Mori M, De Smaele E, Canettieri G, Capalbo C, Maroder M, Screpanti I, Kool M, Pfister SM, Guardavaccaro D, Gulino A, Di Marcotullio L
Nature communications9(1)976(2018 03)

Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors.
Premarathne S, Murtaza M, Matigian N, Jolly LA, Wood SA
Scientific reports7(1)8109(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Itch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15574	NM_008395.3 Latest version!	Mus musculus itchy, E3 ubiquitin protein ligase (Itch), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
OMu15574	NM_001243712.1 Latest version!	Mus musculus itchy, E3 ubiquitin protein ligase (Itch), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itch ( NM_008395.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032421.2 Mus musculus itchy, E3 ubiquitin protein ligase (Itch), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15574
Clone ID Related Accession (Same CDS sequence)	NM_008395.3 , NM_001243712.1
Accession Version	NM_008395.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase Itchy
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ122836.1, BC062934.1 and BF453521.1. On Aug 20, 2011 this sequence version replaced NM_008395.2. Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062934.1, AF037454.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGACA GTGGACCACA GCTTGATTCC ATGGGTAGTC TGACCATGAA ATCTCAACTT 
CAGATCACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAATAATACA 
AACAGTCCCA AGTGGAAGCA GCCCCTCACA GTTATTGTTA CCCCTACGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAG TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACGATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA GGTTGTTACT AACGGTGAAA CGTCATGCTC CGAGAGTACT 
ACACAGAATG ATGATGGCTG CAGAACCAGA GATGATACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC GTCAGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCG 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCGAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAATTC TGACAGTGAT 
GGATCTAGTA CAGGCTCCTT GCCACCAACA AATACAAATG TAAATACAAG TACATCTGAA 
GGAGCAACAT CTGGATTAAT AATTCCTCTT ACTATATCTG GAGGCTCGGG CCCTAGGCCT 
CTGAATACTG TAAGCCAAGC TCCCCTACCA CCTGGGTGGG AGCAGAGAGT AGACCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCAGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGATCAT 
TTCACAAGAA CCACAACATG GCAGAGGCCA ACCTTGGAAT CTGTCCGGAA CTATGAACAG 
TGGCAGCTAC AGCGTAGTCA GCTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TATGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AATTTGATCC GCTTGGTCCG 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACTCAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GAGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACTTA TATAGATCCA CGAACGGGAA AATCAGCCTT AGACAATGGG 
CCCCAGATAG CCTATGTGCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGC 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AGCAGATCAT GAGCTTCAGC CCGCAAGACC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAC ATCAATCCAG ACCACCTGAA ATACTTTCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTG TTTCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCCTGAAT AAACCAGTTG GACTGAAGGA TTTAGAATCT 
ATTGATCCAG AATTTTATAA TTCGCTCATC TGGGTTAAAG AAAACAACAT TGAAGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTAG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTTGTG ACAGAAGAAA ACAAGGAGGA ATACATCAGG 
ATGGTAGCTG AGTGGAGGTT GTCTCGAGGT GTTGAAGAAC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTTCTTCTGT GTGGGATGCA GGAGATTGAT TTGAATGACT GGCAGAGACA TGCCATCTAC 
CGCCACTACA CCAGAACAAG CAAGCAGATC ATGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAATGAGA AGAGGATGAG ACTTCTGCAG TTTGTTACTG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTTAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCT 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACTGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032421.2
CDS	204..2798
Translation	MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEGATSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_008395.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus itchy, E3 ubiquitin protein ligase (Itch), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008395.3

ORF Insert Sequence:

ATGTCTGACA GTGGACCACA GCTTGATTCC ATGGGTAGTC TGACCATGAA ATCTCAACTT 
CAGATCACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAATAATACA 
AACAGTCCCA AGTGGAAGCA GCCCCTCACA GTTATTGTTA CCCCTACGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAG TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACGATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA GGTTGTTACT AACGGTGAAA CGTCATGCTC CGAGAGTACT 
ACACAGAATG ATGATGGCTG CAGAACCAGA GATGATACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC GTCAGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCG 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCGAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAATTC TGACAGTGAT 
GGATCTAGTA CAGGCTCCTT GCCACCAACA AATACAAATG TAAATACAAG TACATCTGAA 
GGAGCAACAT CTGGATTAAT AATTCCTCTT ACTATATCTG GAGGCTCGGG CCCTAGGCCT 
CTGAATACTG TAAGCCAAGC TCCCCTACCA CCTGGGTGGG AGCAGAGAGT AGACCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCAGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGATCAT 
TTCACAAGAA CCACAACATG GCAGAGGCCA ACCTTGGAAT CTGTCCGGAA CTATGAACAG 
TGGCAGCTAC AGCGTAGTCA GCTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TATGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AATTTGATCC GCTTGGTCCG 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACTCAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GAGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACTTA TATAGATCCA CGAACGGGAA AATCAGCCTT AGACAATGGG 
CCCCAGATAG CCTATGTGCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGC 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AGCAGATCAT GAGCTTCAGC CCGCAAGACC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAC ATCAATCCAG ACCACCTGAA ATACTTTCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTG TTTCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCCTGAAT AAACCAGTTG GACTGAAGGA TTTAGAATCT 
ATTGATCCAG AATTTTATAA TTCGCTCATC TGGGTTAAAG AAAACAACAT TGAAGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTAG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTTGTG ACAGAAGAAA ACAAGGAGGA ATACATCAGG 
ATGGTAGCTG AGTGGAGGTT GTCTCGAGGT GTTGAAGAAC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTTCTTCTGT GTGGGATGCA GGAGATTGAT TTGAATGACT GGCAGAGACA TGCCATCTAC 
CGCCACTACA CCAGAACAAG CAAGCAGATC ATGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAATGAGA AGAGGATGAG ACTTCTGCAG TTTGTTACTG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTTAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCT 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACTGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itch ( NM_001243712.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001230641.1 Mus musculus itchy, E3 ubiquitin protein ligase (Itch), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15574
Clone ID Related Accession (Same CDS sequence)	NM_008395.3 , NM_001243712.1
Accession Version	NM_001243712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase Itchy
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845429.4 and AL929588.11. On Aug 20, 2011 this sequence version replaced NR_037196.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK220164.1, SRR1660817.195528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGACA GTGGACCACA GCTTGATTCC ATGGGTAGTC TGACCATGAA ATCTCAACTT 
CAGATCACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAATAATACA 
AACAGTCCCA AGTGGAAGCA GCCCCTCACA GTTATTGTTA CCCCTACGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAG TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACGATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA GGTTGTTACT AACGGTGAAA CGTCATGCTC CGAGAGTACT 
ACACAGAATG ATGATGGCTG CAGAACCAGA GATGATACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC GTCAGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCG 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCGAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAATTC TGACAGTGAT 
GGATCTAGTA CAGGCTCCTT GCCACCAACA AATACAAATG TAAATACAAG TACATCTGAA 
GGAGCAACAT CTGGATTAAT AATTCCTCTT ACTATATCTG GAGGCTCGGG CCCTAGGCCT 
CTGAATACTG TAAGCCAAGC TCCCCTACCA CCTGGGTGGG AGCAGAGAGT AGACCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCAGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGATCAT 
TTCACAAGAA CCACAACATG GCAGAGGCCA ACCTTGGAAT CTGTCCGGAA CTATGAACAG 
TGGCAGCTAC AGCGTAGTCA GCTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TATGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AATTTGATCC GCTTGGTCCG 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACTCAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GAGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACTTA TATAGATCCA CGAACGGGAA AATCAGCCTT AGACAATGGG 
CCCCAGATAG CCTATGTGCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGC 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AGCAGATCAT GAGCTTCAGC CCGCAAGACC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAC ATCAATCCAG ACCACCTGAA ATACTTTCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTG TTTCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCCTGAAT AAACCAGTTG GACTGAAGGA TTTAGAATCT 
ATTGATCCAG AATTTTATAA TTCGCTCATC TGGGTTAAAG AAAACAACAT TGAAGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTAG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTTGTG ACAGAAGAAA ACAAGGAGGA ATACATCAGG 
ATGGTAGCTG AGTGGAGGTT GTCTCGAGGT GTTGAAGAAC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTTCTTCTGT GTGGGATGCA GGAGATTGAT TTGAATGACT GGCAGAGACA TGCCATCTAC 
CGCCACTACA CCAGAACAAG CAAGCAGATC ATGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAATGAGA AGAGGATGAG ACTTCTGCAG TTTGTTACTG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTTAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCT 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACTGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230641.1
CDS	406..3000
Translation	MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEGATSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001243712.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus itchy, E3 ubiquitin protein ligase (Itch), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243712.1

ORF Insert Sequence:

ATGTCTGACA GTGGACCACA GCTTGATTCC ATGGGTAGTC TGACCATGAA ATCTCAACTT 
CAGATCACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAATAATACA 
AACAGTCCCA AGTGGAAGCA GCCCCTCACA GTTATTGTTA CCCCTACGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAG TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACGATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA GGTTGTTACT AACGGTGAAA CGTCATGCTC CGAGAGTACT 
ACACAGAATG ATGATGGCTG CAGAACCAGA GATGATACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC GTCAGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCG 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCGAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAATTC TGACAGTGAT 
GGATCTAGTA CAGGCTCCTT GCCACCAACA AATACAAATG TAAATACAAG TACATCTGAA 
GGAGCAACAT CTGGATTAAT AATTCCTCTT ACTATATCTG GAGGCTCGGG CCCTAGGCCT 
CTGAATACTG TAAGCCAAGC TCCCCTACCA CCTGGGTGGG AGCAGAGAGT AGACCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCAGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGATCAT 
TTCACAAGAA CCACAACATG GCAGAGGCCA ACCTTGGAAT CTGTCCGGAA CTATGAACAG 
TGGCAGCTAC AGCGTAGTCA GCTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TATGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AATTTGATCC GCTTGGTCCG 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACTCAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GAGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACTTA TATAGATCCA CGAACGGGAA AATCAGCCTT AGACAATGGG 
CCCCAGATAG CCTATGTGCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGC 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AGCAGATCAT GAGCTTCAGC CCGCAAGACC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAC ATCAATCCAG ACCACCTGAA ATACTTTCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTG TTTCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCCTGAAT AAACCAGTTG GACTGAAGGA TTTAGAATCT 
ATTGATCCAG AATTTTATAA TTCGCTCATC TGGGTTAAAG AAAACAACAT TGAAGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTAG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTTGTG ACAGAAGAAA ACAAGGAGGA ATACATCAGG 
ATGGTAGCTG AGTGGAGGTT GTCTCGAGGT GTTGAAGAAC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTTCTTCTGT GTGGGATGCA GGAGATTGAT TTGAATGACT GGCAGAGACA TGCCATCTAC 
CGCCACTACA CCAGAACAAG CAAGCAGATC ATGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAATGAGA AGAGGATGAG ACTTCTGCAG TTTGTTACTG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTTAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCT 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACTGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The E3 ubiquitin ligase Itch restricts antigen-driven B cell responses.
The Journal of experimental medicine216(9)2170-2183(2019 Sep)
Moser EK,Roof J,Dybas JM,Spruce LA,Seeholzer SH,Cancro MP,Oliver PM

Notch Activation by Shootin1 Opposing Activities on 2 Ubiquitin Ligases.
Cerebral cortex (New York, N.Y. : 1991)28(9)3115-3128(2018 09)
Sapir T,Levy T,Kozer N,Shin I,Zamor V,Haffner-Krausz R,McGlade JC,Reiner O

The E3 ligases Itch and WWP2 cooperate to limit T
Nature immunology19(7)766-775(2018 07)
Aki D,Li H,Zhang W,Zheng M,Elly C,Lee JH,Zou W,Liu YC

Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis.
Nature communications9(1)976(2018 03)
Infante P,Faedda R,Bernardi F,Bufalieri F,Lospinoso Severini L,Alfonsi R,Mazzà D,Siler M,Coni S,Po A,Petroni M,Ferretti E,Mori M,De Smaele E,Canettieri G,Capalbo C,Maroder M,Screpanti I,Kool M,Pfister SM,Guardavaccaro D,Gulino A,Di Marcotullio L

Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors.
Scientific reports7(1)8109(2017 08)
Premarathne S,Murtaza M,Matigian N,Jolly LA,Wood SA

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Itch cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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