Hcn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcn2
Entrez Gene ID	15166
Full Name	hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms	BCNG2,HAC1,trls
General protein information	Preferred Names hyperpolarization-activated, cyclic nucleotide-gated K+ 2 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 BCNG-2 HAC-1 brain cyclic nucleotide-gated channel 2 hyperpolarization-activated cation channel 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_008226.2	NP_032252.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN2	NP_001185.3
Gallus gallus (chicken)	HCN2	XP_003642915.1
Xenopus tropicalis (tropical clawed frog)	hcn2	XP_002939815.2
Rattus norvegicus (Norway rat)	Hcn2	NP_446136.1
Canis lupus familiaris (dog)	HCN2	XP_005642548.1
Pan troglodytes (chimpanzee)	LOC467640	XP_003952669.1
Mus musculus (house mouse)	Hcn2	NP_032252.1
Danio rerio (zebrafish)	hcn2b	XP_685414.3
Bos taurus (cattle)	HCN2	XP_002689207.2

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of the HCN2 channel increases the arrhythmogenicity induced by hypokalemia.
Oshita K, Kozasa Y, Nakagawa Y, Kuwabara Y, Kuwahara K, Nakagawa T, Nakashima N, Hiraki T, Takano M
The journal of physiological sciences : JPS69(4)653-660(2019 Jul)

Minimal molecular determinants of isoform-specific differences in efficacy in the HCN channel family.
Alvarez-Baron CP, Klenchin VA, Chanda B
The Journal of general physiology150(8)1203-1213(2018 08)

HCN2 Rescues brain defects by enforcing endogenous voltage pre-patterns.
Pai VP, Pietak A, Willocq V, Ye B, Shi NQ, Levin M
Nature communications9(1)998(2018 03)

Loss of HCN2 leads to delayed gastrointestinal motility and reduced energy intake in mice.
Fisher DW, Luu P, Agarwal N, Kurz JE, Chetkovich DM
PloS one13(2)e0193012(2018)

HCN2 channels in the ventral tegmental area regulate behavioral responses to chronic stress.
Zhong P, Vickstrom CR, Liu X, Hu Y, Yu L, Yu HG, Liu QS
eLife7(2018 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22299	NM_008226.2 Latest version!	Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 2 (Hcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcn2 ( NM_008226.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032252.1 Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 2 (Hcn2), mRNA.

Price & Availability↑

CloneID	OMu22299
Clone ID Related Accession (Same CDS sequence)	NM_008226.2
Accession Version	NM_008226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC151828.3, AF064873.1 and CX242892.1. On or before May 4, 2006 this sequence version replaced XM_985431.1, NM_008226.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ225122.1, AB154248.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCGC GCGGGGGCGG CGGGCGGCCG GGCGATAGTC CGGGCACGAC CCCTGCGCCG 
GGGCCGCCGC CACCGCCGCC GCCGCCCGCG CCCCCTCAGC CTCAGCCACC ACCCGCGCCA 
CCCCCGAACC CCACGACCCC CTCGCACCCG GAGTCGGCGG ACGAGCCCGG CCCGCGCGCC 
CGGCTCTGCA GCCGCGACAG CGCCTGCACC CCTGGCGCGG CCAAGGGCGG CGCGAATGGC 
GAGTGCGGGC GCGGGGAGCC GCAGTGCAGC CCCGAGGGCC CCGCGCGCGG CCCCAAGGTT 
TCGTTCTCAT GCCGCGGGGC GGCCTCCGGG CCCTCGGCGG CCGAGGAGGC GGGCAGCGAG 
GAGGCGGGCC CGGCGGGTGA GCCGCGCGGC AGCCAGGCTA GCTTCCTGCA GCGCCAATTC 
GGGGCGCTTC TGCAGCCCGG CGTCAACAAG TTCTCCCTGC GGATGTTCGG CAGCCAGAAG 
GCCGTGGAGC GCGAGCAGGA ACGCGTGAAG TCGGCGGGGG CCTGGATCAT CCACCCCTAC 
AGCGACTTCA GGTTCTACTG GGACTTCACC ATGCTGTTGT TCATGGTGGG AAATCTCATT 
ATCATTCCCG TGGGCATCAC TTTCTTCAAG GACGAGACCA CCGCGCCCTG GATCGTCTTC 
AACGTGGTCT CGGACACTTT CTTCCTCATG GACTTGGTGT TGAACTTCCG CACCGGCATT 
GTTATTGAGG ACAACACGGA GATCATCCTG GACCCCGAGA AGATAAAGAA GAAGTACTTG 
CGTACGTGGT TCGTGGTGGA CTTCGTGTCA TCCATCCCGG TGGACTACAT CTTCCTCATA 
GTGGAGAAGG GAATCGACTC CGAGGTCTAC AAGACAGCGC GTGCTCTGCG CATCGTGCGC 
TTCACCAAGA TCCTCAGTCT GCTGCGGCTG CTGCGGCTAT CACGGCTCAT CCGATATATC 
CACCAGTGGG AAGAGATTTT CCACATGACC TACGACCTGG CAAGTGCAGT GATGCGCATC 
TGTAACCTGA TCAGCATGAT GCTACTGCTC TGCCACTGGG ACGGTTGCCT GCAGTTCCTG 
GTGCCCATGC TGCAAGACTT CCCCAGCGAC TGCTGGGTGT CCATCAACAA CATGGTGAAC 
CACTCGTGGA GCGAGCTCTA CTCGTTCGCG CTCTTCAAGG CCATGAGCCA CATGCTGTGC 
ATCGGCTACG GGCGGCAGGC GCCCGAGAGC ATGACAGACA TCTGGCTGAC CATGCTCAGC 
ATGATCGTAG GCGCCACCTG CTATGCCATG TTCATTGGGC ACGCCACTGC GCTCATCCAG 
TCCCTGGATT CGTCACGGCG CCAATACCAG GAGAAGTACA AGCAAGTAGA GCAATACATG 
TCCTTCCACA AACTGCCCGC TGACTTCCGC CAGAAGATCC ACGATTACTA TGAACACCGG 
TACCAAGGGA AGATGTTTGA TGAGGACAGC ATCCTTGGGG AACTCAACGG GCCACTGCGT 
GAGGAGATTG TGAACTTCAA CTGCCGGAAG CTGGTGGCTT CCATGCCGCT GTTTGCCAAT 
GCAGACCCCA ACTTCGTCAC AGCCATGCTG ACAAAGCTCA AATTTGAGGT CTTCCAGCCT 
GGAGATTACA TCATCCGAGA GGGGACCATC GGGAAGAAGA TGTACTTCAT CCAGCATGGG 
GTGGTGAGCG TGCTCACCAA GGGCAACAAG GAGATGAAGC TGTCGGATGG CTCCTATTTC 
GGGGAGATCT GCTTGCTCAC GAGGGGCCGG CGTACGGCCA GCGTGCGAGC TGACACCTAC 
TGTCGCCTCT ACTCACTGAG TGTGGACAAT TTCAACGAGG TGCTGGAGGA ATACCCCATG 
ATGCGGCGTG CCTTTGAGAC TGTGGCTATT GACCGGCTAG ATCGCATAGG CAAGAAGAAC 
TCCATCTTGC TGCACAAGGT TCAGCATGAT CTCAGCTCAG GTGTGTTCAA CAACCAGGAG 
AATGCCATCA TCCAGGAGAT TGTCAAATAT GACCGTGAGA TGGTGCAGCA GGCAGAGCTT 
GGCCAGCGTG TGGGGCTCTT CCCACCACCG CCACCACCGC AGGTCACATC GGCCATTGCC 
ACCCTACAGC AGGCTGTGGC CATGAGCTTC TGCCCGCAGG TGGCCCGCCC GCTCGTGGGG 
CCCCTGGCGC TAGGCTCCCC ACGCCTAGTG CGCCGCGCGC CCCCAGGGCC TCTGCCTCCT 
GCAGCCTCGC CAGGGCCACC CGCAGCAAGC CCCCCGGCTG CACCCTCGAG CCCTCGGGCA 
CCGCGGACCT CACCCTACGG TGTGCCTGGC TCTCCGGCAA CGCGTGTGGG GCCCGCATTG 
CCCGCACGTC GCCTGAGCCG CGCCTCGCGC CCACTGTCCG CCTCGCAGCC CTCGCTGCCC 
CATGGCGTGC CCGCGCCCAG CCCCGCGGCC TCTGCGCGCC CGGCCAGCAG CTCCACGCCG 
CGCCTGGGAC CCGCACCCAC CGCCCGGACC GCCGCGCCCA GTCCGGACCG CAGGGACTCA 
GCCTCGCCGG GCGCTGCCAG TGGCCTCGAC CCACTGGACT CTGCGCGCTC GCGCCTCTCT 
TCCAACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032252.1
CDS	36..2627
Translation	MDARGGGGRPGDSPGTTPAPGPPPPPPPPAPPQPQPPPAPPPNPTTPSHPESADEPGPRARLCSRDSACTPGAAKGGANGECGRGEPQCSPEGPARGPKVSFSCRGAASGPSAAEEAGSEEAGPAGEPRGSQASFLQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPQVTSAIATLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPPAASPGPPAASPPAAPSSPRAPRTSPYGVPGSPATRVGPALPARRLSRASRPLSASQPSLPHGVPAPSPAASARPASSSTPRLGPAPTARTAAPSPDRRDSASPGAASGLDPLDSARSRLSSNL

Target ORF information:

RefSeq Version	NM_008226.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hyperpolarization-activated, cyclic nucleotide-gated K+ 2 (Hcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008226.2

ORF Insert Sequence:

ATGGATGCGC GCGGGGGCGG CGGGCGGCCG GGCGATAGTC CGGGCACGAC CCCTGCGCCG 
GGGCCGCCGC CACCGCCGCC GCCGCCCGCG CCCCCTCAGC CTCAGCCACC ACCCGCGCCA 
CCCCCGAACC CCACGACCCC CTCGCACCCG GAGTCGGCGG ACGAGCCCGG CCCGCGCGCC 
CGGCTCTGCA GCCGCGACAG CGCCTGCACC CCTGGCGCGG CCAAGGGCGG CGCGAATGGC 
GAGTGCGGGC GCGGGGAGCC GCAGTGCAGC CCCGAGGGCC CCGCGCGCGG CCCCAAGGTT 
TCGTTCTCAT GCCGCGGGGC GGCCTCCGGG CCCTCGGCGG CCGAGGAGGC GGGCAGCGAG 
GAGGCGGGCC CGGCGGGTGA GCCGCGCGGC AGCCAGGCTA GCTTCCTGCA GCGCCAATTC 
GGGGCGCTTC TGCAGCCCGG CGTCAACAAG TTCTCCCTGC GGATGTTCGG CAGCCAGAAG 
GCCGTGGAGC GCGAGCAGGA ACGCGTGAAG TCGGCGGGGG CCTGGATCAT CCACCCCTAC 
AGCGACTTCA GGTTCTACTG GGACTTCACC ATGCTGTTGT TCATGGTGGG AAATCTCATT 
ATCATTCCCG TGGGCATCAC TTTCTTCAAG GACGAGACCA CCGCGCCCTG GATCGTCTTC 
AACGTGGTCT CGGACACTTT CTTCCTCATG GACTTGGTGT TGAACTTCCG CACCGGCATT 
GTTATTGAGG ACAACACGGA GATCATCCTG GACCCCGAGA AGATAAAGAA GAAGTACTTG 
CGTACGTGGT TCGTGGTGGA CTTCGTGTCA TCCATCCCGG TGGACTACAT CTTCCTCATA 
GTGGAGAAGG GAATCGACTC CGAGGTCTAC AAGACAGCGC GTGCTCTGCG CATCGTGCGC 
TTCACCAAGA TCCTCAGTCT GCTGCGGCTG CTGCGGCTAT CACGGCTCAT CCGATATATC 
CACCAGTGGG AAGAGATTTT CCACATGACC TACGACCTGG CAAGTGCAGT GATGCGCATC 
TGTAACCTGA TCAGCATGAT GCTACTGCTC TGCCACTGGG ACGGTTGCCT GCAGTTCCTG 
GTGCCCATGC TGCAAGACTT CCCCAGCGAC TGCTGGGTGT CCATCAACAA CATGGTGAAC 
CACTCGTGGA GCGAGCTCTA CTCGTTCGCG CTCTTCAAGG CCATGAGCCA CATGCTGTGC 
ATCGGCTACG GGCGGCAGGC GCCCGAGAGC ATGACAGACA TCTGGCTGAC CATGCTCAGC 
ATGATCGTAG GCGCCACCTG CTATGCCATG TTCATTGGGC ACGCCACTGC GCTCATCCAG 
TCCCTGGATT CGTCACGGCG CCAATACCAG GAGAAGTACA AGCAAGTAGA GCAATACATG 
TCCTTCCACA AACTGCCCGC TGACTTCCGC CAGAAGATCC ACGATTACTA TGAACACCGG 
TACCAAGGGA AGATGTTTGA TGAGGACAGC ATCCTTGGGG AACTCAACGG GCCACTGCGT 
GAGGAGATTG TGAACTTCAA CTGCCGGAAG CTGGTGGCTT CCATGCCGCT GTTTGCCAAT 
GCAGACCCCA ACTTCGTCAC AGCCATGCTG ACAAAGCTCA AATTTGAGGT CTTCCAGCCT 
GGAGATTACA TCATCCGAGA GGGGACCATC GGGAAGAAGA TGTACTTCAT CCAGCATGGG 
GTGGTGAGCG TGCTCACCAA GGGCAACAAG GAGATGAAGC TGTCGGATGG CTCCTATTTC 
GGGGAGATCT GCTTGCTCAC GAGGGGCCGG CGTACGGCCA GCGTGCGAGC TGACACCTAC 
TGTCGCCTCT ACTCACTGAG TGTGGACAAT TTCAACGAGG TGCTGGAGGA ATACCCCATG 
ATGCGGCGTG CCTTTGAGAC TGTGGCTATT GACCGGCTAG ATCGCATAGG CAAGAAGAAC 
TCCATCTTGC TGCACAAGGT TCAGCATGAT CTCAGCTCAG GTGTGTTCAA CAACCAGGAG 
AATGCCATCA TCCAGGAGAT TGTCAAATAT GACCGTGAGA TGGTGCAGCA GGCAGAGCTT 
GGCCAGCGTG TGGGGCTCTT CCCACCACCG CCACCACCGC AGGTCACATC GGCCATTGCC 
ACCCTACAGC AGGCTGTGGC CATGAGCTTC TGCCCGCAGG TGGCCCGCCC GCTCGTGGGG 
CCCCTGGCGC TAGGCTCCCC ACGCCTAGTG CGCCGCGCGC CCCCAGGGCC TCTGCCTCCT 
GCAGCCTCGC CAGGGCCACC CGCAGCAAGC CCCCCGGCTG CACCCTCGAG CCCTCGGGCA 
CCGCGGACCT CACCCTACGG TGTGCCTGGC TCTCCGGCAA CGCGTGTGGG GCCCGCATTG 
CCCGCACGTC GCCTGAGCCG CGCCTCGCGC CCACTGTCCG CCTCGCAGCC CTCGCTGCCC 
CATGGCGTGC CCGCGCCCAG CCCCGCGGCC TCTGCGCGCC CGGCCAGCAG CTCCACGCCG 
CGCCTGGGAC CCGCACCCAC CGCCCGGACC GCCGCGCCCA GTCCGGACCG CAGGGACTCA 
GCCTCGCCGG GCGCTGCCAG TGGCCTCGAC CCACTGGACT CTGCGCGCTC GCGCCTCTCT 
TCCAACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of the HCN2 channel increases the arrhythmogenicity induced by hypokalemia.
The journal of physiological sciences : JPS69(4)653-660(2019 Jul)
Oshita K,Kozasa Y,Nakagawa Y,Kuwabara Y,Kuwahara K,Nakagawa T,Nakashima N,Hiraki T,Takano M

Minimal molecular determinants of isoform-specific differences in efficacy in the HCN channel family.
The Journal of general physiology150(8)1203-1213(2018 08)
Alvarez-Baron CP,Klenchin VA,Chanda B

HCN2 Rescues brain defects by enforcing endogenous voltage pre-patterns.
Nature communications9(1)998(2018 03)
Pai VP,Pietak A,Willocq V,Ye B,Shi NQ,Levin M

Loss of HCN2 leads to delayed gastrointestinal motility and reduced energy intake in mice.
PloS one13(2)e0193012(2018)
Fisher DW,Luu P,Agarwal N,Kurz JE,Chetkovich DM

HCN2 channels in the ventral tegmental area regulate behavioral responses to chronic stress.
eLife7(2018 01)
Zhong P,Vickstrom CR,Liu X,Hu Y,Yu L,Yu HG,Liu QS