Grin2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grin2a
Entrez Gene ID	14811
Full Name	glutamate receptor, ionotropic, NMDA2A (epsilon 1)
Synonyms	GluN2A,NMDAR2A,NR2A
General protein information	Preferred Names glutamate receptor, ionotropic, NMDA2A (epsilon 1) Names glutamate receptor ionotropic, NMDA 2A GluRepsilon1 N-methyl D-aspartate receptor subtype 2A glutamate [NMDA] receptor subunit epsilon-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A1\|16 5.28 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008170.2	NP_032196.2	glutamate receptor ionotropic, NMDA 2A precursor
NM_008170.3	NP_032196.2	glutamate receptor ionotropic, NMDA 2A precursor
NM_008170.4	NP_032196.2	glutamate receptor ionotropic, NMDA 2A precursor
XM_006521794.2	XP_006521857.1	glutamate receptor ionotropic, NMDA 2A isoform X1
XM_006521795.3	XP_006521858.1	glutamate receptor ionotropic, NMDA 2A isoform X2
XM_006521795.4	XP_006521858.1	glutamate receptor ionotropic, NMDA 2A isoform X1
XM_006521794.3	XP_006521857.1	glutamate receptor ionotropic, NMDA 2A isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Grin2a	NP_032196.2
Gallus gallus (chicken)	GRIN2A	XP_425252.3
Danio rerio (zebrafish)	grin2aa	XP_691754.3
Homo sapiens (human)	GRIN2A	NP_001127879.1
Xenopus tropicalis (tropical clawed frog)	grin2a	XP_002939231.2
Macaca mulatta (Rhesus monkey)	LOC100429880	XP_002802440.1
Rattus norvegicus (Norway rat)	Grin2a	NP_036705.3
Canis lupus familiaris (dog)	GRIN2A	XP_547132.2
Pan troglodytes (chimpanzee)	GRIN2A	NP_001029361.1

Gene Ontology
Bibliography

Related articles in PubMed

Control of aversion by glycine-gated GluN1/GluN3A NMDA receptors in the adult medial habenula.
Otsu Y, Darcq E, Pietrajtis K, Mátyás F, Schwartz E, Bessaih T, Abi Gerges S, Rousseau CV, Grand T, Dieudonné S, Paoletti P, Acsády L, Agulhon C, Kieffer BL, Diana MA
Science (New York, N.Y.)366(6462)250-254(2019 10)

N-terminal alternative splicing of GluN1 regulates the maturation of excitatory synapses and seizure susceptibility.
Liu H, Wang H, Peterson M, Zhang W, Hou G, Zhang ZW
Proceedings of the National Academy of Sciences of the United States of America116(42)21207-21212(2019 Oct)

Autism candidate gene DIP2A regulates spine morphogenesis via acetylation of cortactin.
Ma J, Zhang LQ, He ZX, He XX, Wang YJ, Jian YL, Wang X, Zhang BB, Su C, Lu J, Huang BQ, Zhang Y, Wang GY, Guo WX, Qiu DL, Mei L, Xiong WC, Zheng YW, Zhu XJ
PLoS biology17(10)e3000461(2019 10)

Role of GluN2A NMDA receptor in homocysteine-induced prostaglandin E2 release from neurons.
Rajagopal S, Fitzgerald AA, Deep SN, Paul S, Poddar R
Journal of neurochemistry150(1)44-55(2019 Jul)

Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.
Yang CY, Yu TH, Wen WL, Ling P, Hsu KS
The Journal of neuroscience : the official journal of the Society for Neuroscience39(25)4959-4975(2019 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Grin2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grin2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22286	XM_006521794.3 Latest version!	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OMu51875	XM_006521795.4 Latest version!	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu22286	NM_008170.4 Latest version!	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$727.30 $1039.00
View Old Accession Versions >>
OMu22286	NM_008170.2	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OMu22286	NM_008170.3	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$727.30 $1039.00
OMu51875	XM_006521795.3	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu22286	XM_006521794.2	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grin2a ( NM_008170.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032196.2 Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.

Price & Availability↑

CloneID	OMu22286
Clone ID Related Accession (Same CDS sequence)	NM_008170.2 , NM_008170.3 , NM_008170.4 , XM_006521794.2 , XM_006521794.3
Accession Version	NM_008170.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Mus musculus(house mouse)
Product	glutamate receptor ionotropic, NMDA 2A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D10217.2. On or before Sep 3, 2004 this sequence version replaced XM_358759.2, NM_008170.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10217.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032196.2
CDS	1..4395
Translation	MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRAADFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGENMNELQTFVANRHKDSLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSEVERTYVKTKASSPRDKIYTIDGEKEPSFHLDPPQFIENIVLPENVDFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEAYQQDWSQNNALQFQKNKLKINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNNMYSTPRVLNSCSNRRVYKKMPSIESDV

Target ORF information:

RefSeq Version	NM_008170.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008170.2

ORF Insert Sequence:

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2a ( XM_006521794.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521857.1 Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.

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CloneID	OMu22286
Clone ID Related Accession (Same CDS sequence)	NM_008170.2 , NM_008170.3 , NM_008170.4 , XM_006521794.2 , XM_006521794.3
Accession Version	XM_006521794.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423324800000
Organism	Mus musculus(House mouse)
Product	glutamate receptor ionotropic, NMDA 2A isoform X1
Comment

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006521857.1

Translation

MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRAADFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGENMNELQTFVANRHKDSLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSEVERTYVKTKASSPRDKIYTIDGEKEPSFHLDPPQFIENIVLPENVDFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEAYQQDWSQNNALQFQKNKLKINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNNMYSTPRVLNSCSNRRVYKKMPSIESDV

Target ORF information:

RefSeq Version	XM_006521794.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521794.2

ORF Insert Sequence:

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2a ( XM_006521794.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521857.1 Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22286
Clone ID Related Accession (Same CDS sequence)	NM_008170.2 , NM_008170.3 , NM_008170.4 , XM_006521794.2 , XM_006521794.3
Accession Version	XM_006521794.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	glutamate receptor ionotropic, NMDA 2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521794.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521857.1
CDS	1888..6282
Translation	MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRAADFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGENMNELQTFVANRHKDSLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSEVERTYVKTKASSPRDKIYTIDGEKEPSFHLDPPQFIENIVLPENVDFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEAYQQDWSQNNALQFQKNKLKINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNNMYSTPRVLNSCSNRRVYKKMPSIESDV

Target ORF information:

RefSeq Version	XM_006521794.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521794.3

ORF Insert Sequence:

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2a ( XM_006521795.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521858.1 Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X2, mRNA.

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CloneID	OMu51875
Clone ID Related Accession (Same CDS sequence)	XM_006521795.3 , XM_006521795.4
Accession Version	XM_006521795.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	glutamate receptor ionotropic, NMDA 2A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521795.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521858.1
CDS	520..2694
Translation	MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGC

Target ORF information:

RefSeq Version	XM_006521795.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521795.3

ORF Insert Sequence:

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2a ( XM_006521795.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521858.1 Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.

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CloneID	OMu51875
Clone ID Related Accession (Same CDS sequence)	XM_006521795.3 , XM_006521795.4
Accession Version	XM_006521795.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	glutamate receptor ionotropic, NMDA 2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521795.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521858.1
CDS	1471..3645
Translation	MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGC

Target ORF information:

RefSeq Version	XM_006521795.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521795.4

ORF Insert Sequence:

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2a ( NM_008170.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032196.2 Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.

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CloneID	OMu22286
Clone ID Related Accession (Same CDS sequence)	NM_008170.2 , NM_008170.3 , NM_008170.4 , XM_006521794.2 , XM_006521794.3
Accession Version	NM_008170.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1552233600000
Organism	Mus musculus(house mouse)
Product	glutamate receptor ionotropic, NMDA 2A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164163.2, AC154190.2, AC121974.3 and AC126022.4. On or before Apr 18, 2018 this sequence version replaced XM_006521794.3, NM_008170.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: D10217.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032196.2
CDS	520..4914
Translation	MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRAADFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGENMNELQTFVANRHKDSLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSEVERTYVKTKASSPRDKIYTIDGEKEPSFHLDPPQFIENIVLPENVDFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEAYQQDWSQNNALQFQKNKLKINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNNMYSTPRVLNSCSNRRVYKKMPSIESDV

Target ORF information:

RefSeq Version	NM_008170.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008170.3

ORF Insert Sequence:

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2a ( NM_008170.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032196.2 Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.

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CloneID	OMu22286
Clone ID Related Accession (Same CDS sequence)	NM_008170.2 , NM_008170.3 , NM_008170.4 , XM_006521794.2 , XM_006521794.3
Accession Version	NM_008170.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Mus musculus(house mouse)
Product	glutamate receptor ionotropic, NMDA 2A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164163.2, AC154190.2, AC121974.3 and AC126022.4. On Jun 19, 2019 this sequence version replaced NM_008170.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR7652917.747001.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032196.2
CDS	1201..5595
Translation	MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRAADFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGENMNELQTFVANRHKDSLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSEVERTYVKTKASSPRDKIYTIDGEKEPSFHLDPPQFIENIVLPENVDFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEAYQQDWSQNNALQFQKNKLKINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNNMYSTPRVLNSCSNRRVYKKMPSIESDV

Target ORF information:

RefSeq Version	NM_008170.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1) (Grin2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008170.4

ORF Insert Sequence:

ATGGGCAGAC TGGGCTACTG GACCTTGCTG GTATTGCCGG CCCTTCTGGT CTGGCACGGT 
CCGGCGCAGA ACGCGGCGGC GGAGAAGGGT ACTCCAGCGC TGAACATTGC GGTGCTGCTG 
GGTCACAGCC ACGACGTGAC AGAACGCGAA CTTCGAAATC TGTGGGGCCC GGAGCAGGCA 
ACCGGCTTGC CCCTGGATGT GAACGTGGTG GCGTTATTGA TGAACCGCAC TGACCCTAAG 
AGCCTCATCA CGCATGTGTG CGACCTCATG TCCGGGGCGC GCATCCATGG CTTGGTGTTT 
GGAGATGATA CGGACCAAGA GGCTGTGGCT CAGATGCTGG ATTTTATCTC CTCACAGACT 
TTCATCCCCA TCTTGGGCAT TCATGGGGGT GCATCTATGA TCATGGCTGA CAAGGATCCG 
ACATCCACGT TCTTCCAGTT TGGAGCTTCC ATCCAGCAGC AAGCCACGGT CATGCTGAAG 
ATCATGCAGG ACTATGACTG GCATGTCTTC TCCTTGGTCA CCACCATCTT CCCTGGCTAC 
AGAGACTTCA TCAGCTTCAT CAAGACAACA GTGGACAACA GCTTTGTGGG CTGGGATATG 
CAGAACGTGA TCACACTGGA CACCTCTTTT GAGGACGCCA AGACACAGGT CCAGCTGAAG 
AAGATCCACT CCTCTGTCAT CCTGCTCTAC TGCTCCAAGG ATGAGGCTGT CCTCATCCTG 
AGCGAGGCTC GCTCTCTTGG CCTCACCGGC TACGATTTCT TCTGGATTGT CCCCAGTTTG 
GTCTCCGGGA ACACAGAGCT CATCCCCAAA GAGTTTCCAT CGGGTCTCAT TTCAGTCTCT 
TACGACGACT GGGACTACAG TCTGGAGGCA AGAGTGAGAG ACGGTCTTGG GATCTTAACC 
ACTGCCGCAT CTTCCATGTT GGAGAAATTC TCCTACATTC CCGAGGCCAA GGCCAGCTGC 
TACGGGCAGA CAGAGAAGCC GGAGACCCCG CTACACACTC TGCACCAATT TATGGTCAAT 
GTGACTTGGG ATGGCAAGGA CTTGTCCTTC ACTGAGGAAG GCTATCAGGT GCACCCCAGG 
CTTGTGGTGA TCGTGCTGAA TAAGGACCGG GAATGGGAAA AGGTGGGCAA GTGGGAGAAT 
CAGACTCTGA GCCTGCGGCA TGCTGTGTGG CCAAGGTATA AGTCCTTTTC TGACTGTGAG 
CCAGATGACA ACCACCTCAG CATTGTCACC TTGGAGGAAG CCCCCTTCGT CATCGTAGAA 
GACATAGACC CACTGACTGA GACCTGCGTC AGGAACACGG TACCCTGTCG GAAGTTTGTC 
AAGATCAACA ATTCAACCAA CGAAGGGATG AATGTGAAGA AGTGCTGCAA GGGGTTCTGC 
ATCGACATCC TTAAGAAACT GTCCAGAACT GTAAAGTTCA CCTATGACCT CTACCTGGTG 
ACCAATGGGA AGCATGGGAA AAAGGTTAAC AATGTGTGGA ATGGAATGAT AGGCGAAGTG 
GTCTATCAAC GAGCAGTTAT GGCCGTGGGC TCCCTCACCA TCAATGAGGA GCGTTCAGAA 
GTGGTGGACT TCTCCGTGCC CTTTGTGGAG ACAGGAATCA GTGTCATGGT CTCCAGGAGT 
AATGGCACTG TTTCCCCTTC TGCTTTCCTT GAACCCTTCA GCGCCTCTGT CTGGGTGATG 
ATGTTCGTGA TGCTGCTCAT TGTCTCTGCC ATTGCTGTCT TCGTTTTTGA ATACTTCAGT 
CCTGTTGGAT ACAACAGAAA CTTAGCCAAA GGGAAAGCTC CCCATGGGCC TTCTTTTACC 
ATTGGAAAAG CTATATGGCT CCTCTGGGGC CTGGTCTTCA ACAATTCTGT GCCCGTCCAG 
AATCCTAAAG GCACAACCAG CAAGATAATG GTATCAGTGT GGGCCTTCTT TGCCGTCATC 
TTCCTTGCAA GTTACACAGC CAACCTGGCT GCCTTCATGA TCCAGGAGGA GTTTGTGGAC 
CAAGTGACTG GCCTCAGTGA CAAGAAGTTC CAGAGACCTC ATGACTATTC TCCGCCTTTC 
CGATTTGGGA CAGTACCCAA TGGAAGTACA GAAAGGAATA TTCGTAACAA CTACCCCTAT 
ATGCACCAGT ACATGACCAA ATTCAACCAG AGGGGCGTAG AGGATGCCTT GGTCAGCTTG 
AAAACTGGGA AGTTGGACGC TTTCATCTAT GACGCAGCTG TCTTGAACTA CAAGGCCGGG 
AGGGATGAAG GCTGTAAACT GGTGACCATT GGGAGCGGGT ACATCTTTGC CACCACAGGC 
TATGGAATTG CTCTGCAGAA GGGCTCACCC TGGAAGAGGC AGATTGACCT CGCTCTGCTC 
CAGTTTGTTG GTGACGGTGA GATGGAAGAG CTGGAGACAC TGTGGCTTAC GGGCATCTGC 
CACAACGAGA AGAATGAGGT GATGAGCAGC CAGCTGGACA TCGACAACAT GGCAGGAGTT 
TTCTACATGC TGGCTGCAGC TATGGCCCTC AGCCTCATCA CCTTCATCTG GGAGCACCTC 
TTCTACTGGA AGCTGCGCTT CTGCTTCACA GGCGTGTGTT CTGACCGGCC CGGGCTGCTC 
TTCTCCATCA GCAGGGGCAT CTACAGTTGC ATCCATGGAG TGCACATTGA AGAAAAGAAG 
AAGTCTCCAG ACTTCAATCT GACTGGGTCA CAGAGCAACA TGCTAAAGCT TCTCCGCTCA 
GCTAAAAACA TCTCCAACAT GTCCAACATG AACTCCTCGC GAATGGACTC ACCCAAAAGA 
GCTGCTGACT TCATCCAAAG AGGCTCACTT ATTGTGGACA TGGTTTCAGA CAAGGGAAAT 
TTGATATACT CAGATAACAG GTCCTTTCAA GGGAAGGACA GTATATTTGG AGAAAACATG 
AATGAACTGC AAACATTTGT GGCCAACAGG CACAAGGATA GTCTCAGTAA CTATGTGTTT 
CAGGGACAGC ATCCTCTCAC TCTCAATGAG TCCAACCCCA ACACAGTGGA GGTGGCTGTC 
AGCACTGAAT CCAAAGGGAA CTCCCGACCC CGGCAGCTTT GGAAGAAATC CATGGAGTCT 
CTACGCCAGG ATTCTCTAAA CCAGAACCCA GTCTCCCAGA GGGATGAGAA GACTGCAGAG 
AATAGGACCC ACTCCCTAAA GAGTCCTAGG TATCTTCCAG AAGAGGTAGC CCATTCTGAC 
ATTTCTGAAA CCTCAAGCCG GGCCACATGC CACAGGGAGC CAGATAATAA TAAGAACCAC 
AAGACCAAGG ATAACTTCAA AAGGTCAATG GCCTCTAAAT ACCCCAAGGA CTGTAGTGAG 
GTTGAACGTA CCTACGTGAA AACCAAAGCA AGTTCTCCCA GGGATAAGAT CTACACCATC 
GATGGTGAGA AGGAGCCCAG CTTCCACTTA GATCCTCCAC AGTTCATTGA AAACATAGTC 
TTGCCTGAGA ATGTGGACTT CCCAGATACC TACCAAGATC ACAATGAGAA TTTCCGCAAG 
GGGGACTCCA CACTGCCCAT GAACAGGAAC CCACTACACA ATGAAGATGG GCTTCCCAAC 
AATGACCAGT ATAAACTCTA TGCCAAGCAC TTTACCTTGA AAGACAAGGG TTCCCCACAT 
AGTGAGGGCA GTGATCGATA TCGGCAGAAC TCCACGCATT GCAGAAGCTG CCTCTCAAAC 
CTGCCCACCT ACTCAGGCCA CTTTACCATG AGATCTCCTT TCAAGTGTGA TGCCTGTCTG 
CGGATGGGGA ACCTCTATGA CATTGATGAA GACCAGATGC TTCAGGAGAC AGGCAACCCA 
GCTACTCGTG AGGAGGCCTA CCAGCAGGAC TGGTCACAGA ACAACGCCCT CCAGTTCCAG 
AAGAACAAGC TAAAGATTAA TCGACAGCAC TCCTATGATA ACATTCTCGA CAAACCCAGG 
GAGATAGACC TTAGCAGGCC CTCTCGTAGC ATAAGCCTCA AGGACAGGGA AAGGCTACTG 
GAGGGCAACT TATACGGGAG CCTGTTCAGT GTCCCCTCAA GCAAACTCTT GGGGAACAAA 
AGCTCCCTTT TCCCCCAAGG TCTGGAGGAC AGCAAGAGGA GCAAATCTCT CTTGCCAGAC 
CATACCTCTG ATAATCCTTT CCTCCACACG TACGGGGATG ACCAACGCTT AGTTATTGGG 
AGATGTCCCT CGGACCCTTA CAAACACTCA TTGCCATCAC AGGCAGTAAA TGACAGCTAT 
CTTCGGTCAT CCTTGAGGTC AACAGCATCA TATTGCTCCA GGGACAGTCG GGGCCACAGT 
GATGTGTATA TTTCAGAGCA TGTTATGCCT TATGCTGCAA ATAAGAATAA CATGTACTCT 
ACCCCCAGGG TTTTAAATTC CTGCAGCAAT AGACGTGTGT ACAAGAAAAT GCCTAGTATT 
GAATCTGATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Control of aversion by glycine-gated GluN1/GluN3A NMDA receptors in the adult medial habenula.
Science (New York, N.Y.)366(6462)250-254(2019 10)
Otsu Y,Darcq E,Pietrajtis K,Mátyás F,Schwartz E,Bessaih T,Abi Gerges S,Rousseau CV,Grand T,Dieudonné S,Paoletti P,Acsády L,Agulhon C,Kieffer BL,Diana MA

N-terminal alternative splicing of GluN1 regulates the maturation of excitatory synapses and seizure susceptibility.
Proceedings of the National Academy of Sciences of the United States of America116(42)21207-21212(2019 Oct)
Liu H,Wang H,Peterson M,Zhang W,Hou G,Zhang ZW

Autism candidate gene DIP2A regulates spine morphogenesis via acetylation of cortactin.
PLoS biology17(10)e3000461(2019 10)
Ma J,Zhang LQ,He ZX,He XX,Wang YJ,Jian YL,Wang X,Zhang BB,Su C,Lu J,Huang BQ,Zhang Y,Wang GY,Guo WX,Qiu DL,Mei L,Xiong WC,Zheng YW,Zhu XJ

Role of GluN2A NMDA receptor in homocysteine-induced prostaglandin E2 release from neurons.
Journal of neurochemistry150(1)44-55(2019 Jul)
Rajagopal S,Fitzgerald AA,Deep SN,Paul S,Poddar R

Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.
The Journal of neuroscience : the official journal of the Society for Neuroscience39(25)4959-4975(2019 Jun)
Yang CY,Yu TH,Wen WL,Ling P,Hsu KS

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Grin2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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