Fdft1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fdft1
Entrez Gene ID	14137
Full Name	farnesyl diphosphate farnesyl transferase 1
Synonyms	SQS,SS
General protein information	Preferred Names farnesyl diphosphate farnesyl transferase 1 Names squalene synthase FPP:FPP farnesyltransferase farnesyl-diphosphate farnesyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 D1\|14 33.24 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010191.3	NP_034321.2	squalene synthase
NM_001360211.1	NP_001347140.1	squalene synthase
XM_006518547.3	XP_006518610.1	squalene synthase isoform X2
XM_006518547.4	XP_006518610.1	squalene synthase isoform X2
XM_017315844.1	XP_017171333.1	squalene synthase isoform X1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os07g0200700	NP_001059129.1
Canis lupus familiaris (dog)	FDFT1	XP_534557.2
Neurospora crassa	NCU06054	XP_959817.1
Danio rerio (zebrafish)	si:ch73-107c13.2	NP_001189454.1
Eremothecium gossypii	AGOS_AFR444C	NP_985991.1
Homo sapiens (human)	FDFT1	NP_001274671.1
Mus musculus (house mouse)	Fdft1	NP_034321.2
Rattus norvegicus (Norway rat)	Fdft1	NP_062111.1
Gallus gallus (chicken)	FDFT1	NP_001034383.1
Schizosaccharomyces pombe (fission yeast)	erg9	NP_595363.1
Arabidopsis thaliana (thale cress)	SQS2	NP_195191.2
Oryza sativa (rice)	Os03g0805100	NP_001051625.1
Kluyveromyces lactis	KLLA0D08866g	XP_453457.1
Magnaporthe oryzae (rice blast fungus)	MGG_09239	XP_003709863.1
Pan troglodytes (chimpanzee)	FDFT1	XP_001141492.1
Macaca mulatta (Rhesus monkey)	FDFT1	XP_001086419.1
Bos taurus (cattle)	FDFT1	NP_001013022.1
Saccharomyces cerevisiae (baker's yeast)	ERG9	NP_012060.1
Arabidopsis thaliana (thale cress)	SQS1	NP_195190.1
Xenopus tropicalis (tropical clawed frog)	fdft1	NP_001135642.2

Gene Ontology
Bibliography

Related articles in PubMed

Maf links Neuregulin1 signaling to cholesterol synthesis in myelinating Schwann cells.
Kim M, Wende H, Walcher J, Kühnemund J, Cheret C, Kempa S, McShane E, Selbach M, Lewin GR, Birchmeier C
Genes & development32(9-10)645-657(2018 05)

Cytoplasmic cleavage of DPPA3 is required for intracellular trafficking and cleavage-stage development in mice.
Shin SW, Vogt EJ, Jimenez-Movilla M, Baibakov B, Dean J
Nature communications8(1)1643(2017 11)

Mfsd2a Is a Transporter for the Essential ω-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.
Wong BH, Chan JP, Cazenave-Gassiot A, Poh RW, Foo JC, Galam DL, Ghosh S, Nguyen LN, Barathi VA, Yeo SW, Luu CD, Wenk MR, Silver DL
The Journal of biological chemistry291(20)10501-14(2016 May)

A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.
Şişecioğlu M, Budak H, Geffers L, Çankaya M, Çiftci M, Thaller C, Eichele G, Küfrevioğlu Öİ, Özdemir H
Journal of lipid research56(8)1551-9(2015 Aug)

Plasma cholesterol-lowering and transient liver dysfunction in mice lacking squalene synthase in the liver.
Nagashima S, Yagyu H, Tozawa R, Tazoe F, Takahashi M, Kitamine T, Yamamuro D, Sakai K, Sekiya M, Okazaki H, Osuga J, Honda A, Ishibashi S
Journal of lipid research56(5)998-1005(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fdft1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fdft1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu49660	XM_006518547.4 Latest version!	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu05703	NM_010191.3 Latest version!	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00
OMu05703	NM_001360211.1 Latest version!	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00
OMu05703	XM_017315844.1 Latest version!	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00
View Old Accession Versions >>
OMu49660	XM_006518547.3	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fdft1 ( XM_006518547.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518610.1 Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu49660
Clone ID Related Accession (Same CDS sequence)	XM_006518547.3 , XM_006518547.4
Accession Version	XM_006518547.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	squalene synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGTG GGATGGCAGA ATTTGTAGAC AAGGATGTGA CCTCCAAACA GGACTGGGAC 
AAGTACTGCC ACTACGTTGC TGGGCTGGTG GGAATTGGCC TTTCTCGTCT ATTCTCTGCC 
TCAGAGTTTG AAGACCCCAT AGTTGGTGAA GACATAGAGT GTGCCAACTC AATGGGTCTG 
TTCCTGCAGA AAACAAATAT CATTCGTGAT TATCTGGAAG ACCAACAGGA AGGAAGGAAG 
TTTTGGCCTC AGGAGGTGTG GGGCAGATAC ATTAAGAAGT TGGAAGACTT TGCTAAGCCA 
GAGAACGTAG ATGTGGCCGT GCAGTGCTTG AATGAACTCA TAACCAACAC CCTACAGCAC 
ATCCCTGACG TCCTCACCTA CCTGTCAAGG CTCCGGAACC AGAGTGTGTT TAACTTCTGT 
GCTATTCCAC AGGTAATGGC CATTGCCACA CTGGCTGCCT GTTACAATAA CCAGCAGGTA 
TTCAAAGGAG TAGTGAAGAT TCGGAAGGGG CAAGCAGTCA CCCTCATGAT GGATGCCACC 
AACATGCCTG CCGTCAAAGC TATCATATAC CAGTACATAG AAGAGATTTA TCACCGGATC 
CCCAACTCAG ACCCATCATC AAGCAAAACC AAGCAGGTCA TCTCCAAGAT CAGGACACAG 
AACCTTCCCA ACTGCCAGCT CATCTCCCGA AGCCACTACT CCCCCATTTA CCTGTCATTT 
ATCATGCTCT TGGCTGCCCT GAGCTGGCAG TACCTGAGCA CCCTGTCCCA GGTCACAGAA 
GACTATGTCC AGAGAGAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518610.1
CDS	2480..3283
Translation	MGCGMAEFVDKDVTSKQDWDKYCHYVAGLVGIGLSRLFSASEFEDPIVGEDIECANSMGLFLQKTNIIRDYLEDQQEGRKFWPQEVWGRYIKKLEDFAKPENVDVAVQCLNELITNTLQHIPDVLTYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIYQYIEEIYHRIPNSDPSSSKTKQVISKIRTQNLPNCQLISRSHYSPIYLSFIMLLAALSWQYLSTLSQVTEDYVQREH

Target ORF information:

RefSeq Version	XM_006518547.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518547.3

ORF Insert Sequence:

ATGGGGTGTG GGATGGCAGA ATTTGTAGAC AAGGATGTGA CCTCCAAACA GGACTGGGAC 
AAGTACTGCC ACTACGTTGC TGGGCTGGTG GGAATTGGCC TTTCTCGTCT ATTCTCTGCC 
TCAGAGTTTG AAGACCCCAT AGTTGGTGAA GACATAGAGT GTGCCAACTC AATGGGTCTG 
TTCCTGCAGA AAACAAATAT CATTCGTGAT TATCTGGAAG ACCAACAGGA AGGAAGGAAG 
TTTTGGCCTC AGGAGGTGTG GGGCAGATAC ATTAAGAAGT TGGAAGACTT TGCTAAGCCA 
GAGAACGTAG ATGTGGCCGT GCAGTGCTTG AATGAACTCA TAACCAACAC CCTACAGCAC 
ATCCCTGACG TCCTCACCTA CCTGTCAAGG CTCCGGAACC AGAGTGTGTT TAACTTCTGT 
GCTATTCCAC AGGTAATGGC CATTGCCACA CTGGCTGCCT GTTACAATAA CCAGCAGGTA 
TTCAAAGGAG TAGTGAAGAT TCGGAAGGGG CAAGCAGTCA CCCTCATGAT GGATGCCACC 
AACATGCCTG CCGTCAAAGC TATCATATAC CAGTACATAG AAGAGATTTA TCACCGGATC 
CCCAACTCAG ACCCATCATC AAGCAAAACC AAGCAGGTCA TCTCCAAGAT CAGGACACAG 
AACCTTCCCA ACTGCCAGCT CATCTCCCGA AGCCACTACT CCCCCATTTA CCTGTCATTT 
ATCATGCTCT TGGCTGCCCT GAGCTGGCAG TACCTGAGCA CCCTGTCCCA GGTCACAGAA 
GACTATGTCC AGAGAGAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fdft1 ( XM_006518547.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518610.1 Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu49660
Clone ID Related Accession (Same CDS sequence)	XM_006518547.3 , XM_006518547.4
Accession Version	XM_006518547.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	squalene synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518547.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGTG GGATGGCAGA ATTTGTAGAC AAGGATGTGA CCTCCAAACA GGACTGGGAC 
AAGTACTGCC ACTACGTTGC TGGGCTGGTG GGAATTGGCC TTTCTCGTCT ATTCTCTGCC 
TCAGAGTTTG AAGACCCCAT AGTTGGTGAA GACATAGAGT GTGCCAACTC AATGGGTCTG 
TTCCTGCAGA AAACAAATAT CATTCGTGAT TATCTGGAAG ACCAACAGGA AGGAAGGAAG 
TTTTGGCCTC AGGAGGTGTG GGGCAGATAC ATTAAGAAGT TGGAAGACTT TGCTAAGCCA 
GAGAACGTAG ATGTGGCCGT GCAGTGCTTG AATGAACTCA TAACCAACAC CCTACAGCAC 
ATCCCTGACG TCCTCACCTA CCTGTCAAGG CTCCGGAACC AGAGTGTGTT TAACTTCTGT 
GCTATTCCAC AGGTAATGGC CATTGCCACA CTGGCTGCCT GTTACAATAA CCAGCAGGTA 
TTCAAAGGAG TAGTGAAGAT TCGGAAGGGG CAAGCAGTCA CCCTCATGAT GGATGCCACC 
AACATGCCTG CCGTCAAAGC TATCATATAC CAGTACATAG AAGAGATTTA TCACCGGATC 
CCCAACTCAG ACCCATCATC AAGCAAAACC AAGCAGGTCA TCTCCAAGAT CAGGACACAG 
AACCTTCCCA ACTGCCAGCT CATCTCCCGA AGCCACTACT CCCCCATTTA CCTGTCATTT 
ATCATGCTCT TGGCTGCCCT GAGCTGGCAG TACCTGAGCA CCCTGTCCCA GGTCACAGAA 
GACTATGTCC AGAGAGAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518610.1
CDS	4042..4845
Translation	MGCGMAEFVDKDVTSKQDWDKYCHYVAGLVGIGLSRLFSASEFEDPIVGEDIECANSMGLFLQKTNIIRDYLEDQQEGRKFWPQEVWGRYIKKLEDFAKPENVDVAVQCLNELITNTLQHIPDVLTYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIYQYIEEIYHRIPNSDPSSSKTKQVISKIRTQNLPNCQLISRSHYSPIYLSFIMLLAALSWQYLSTLSQVTEDYVQREH

Target ORF information:

RefSeq Version	XM_006518547.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518547.4

ORF Insert Sequence:

ATGGGGTGTG GGATGGCAGA ATTTGTAGAC AAGGATGTGA CCTCCAAACA GGACTGGGAC 
AAGTACTGCC ACTACGTTGC TGGGCTGGTG GGAATTGGCC TTTCTCGTCT ATTCTCTGCC 
TCAGAGTTTG AAGACCCCAT AGTTGGTGAA GACATAGAGT GTGCCAACTC AATGGGTCTG 
TTCCTGCAGA AAACAAATAT CATTCGTGAT TATCTGGAAG ACCAACAGGA AGGAAGGAAG 
TTTTGGCCTC AGGAGGTGTG GGGCAGATAC ATTAAGAAGT TGGAAGACTT TGCTAAGCCA 
GAGAACGTAG ATGTGGCCGT GCAGTGCTTG AATGAACTCA TAACCAACAC CCTACAGCAC 
ATCCCTGACG TCCTCACCTA CCTGTCAAGG CTCCGGAACC AGAGTGTGTT TAACTTCTGT 
GCTATTCCAC AGGTAATGGC CATTGCCACA CTGGCTGCCT GTTACAATAA CCAGCAGGTA 
TTCAAAGGAG TAGTGAAGAT TCGGAAGGGG CAAGCAGTCA CCCTCATGAT GGATGCCACC 
AACATGCCTG CCGTCAAAGC TATCATATAC CAGTACATAG AAGAGATTTA TCACCGGATC 
CCCAACTCAG ACCCATCATC AAGCAAAACC AAGCAGGTCA TCTCCAAGAT CAGGACACAG 
AACCTTCCCA ACTGCCAGCT CATCTCCCGA AGCCACTACT CCCCCATTTA CCTGTCATTT 
ATCATGCTCT TGGCTGCCCT GAGCTGGCAG TACCTGAGCA CCCTGTCCCA GGTCACAGAA 
GACTATGTCC AGAGAGAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fdft1 ( NM_010191.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034321.2 Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05703
Clone ID Related Accession (Same CDS sequence)	NM_010191.3 , NM_001360211.1 , XM_017315844.1
Accession Version	NM_010191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	squalene synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK136159.1 and BC054722.1. On or before Feb 15, 2015 this sequence version replaced XM_006518546.2, NM_010191.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK136159.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTCG TCAAGTGTCT AGGCCACCCG GAGGAGTTCT ATAACCTGCT GCGATTCCGC 
ATGGGAGGCC GGCGGAATTT TATACCCAAG ATGGACCAGG ACTCACTCAG CAGCAGCTTG 
AAGACCTGCT ACAAGTATCT CAATCAGACC AGTCGCAGCT TTGCCGCGGT TATCCAGGCG 
CTGGATGGGG ACATACGGCA CGCCATATGT GTGTTCTACC TGGTTCTCCG AGCCCTGGAT 
ACAGTGGAGG ATGACATGAG CATCAGTGTG GAGAAGAAGA TCCCACTGCT GTGTAACTTC 
CACACTTTCC TCTATGACCC AGAGTGGCGG TTCACTGAGA GCAAGGAGAA GGACCGACAA 
GTGCTGGAGG ACTTCCCCAC GATCTCCCTG GAGTTTAGAA ATTTGGCTGA GAAATATCAA 
ACAGTGATCG ATGACATCTG CCACCGGATG GGGTGTGGGA TGGCAGAATT TGTAGACAAG 
GATGTGACCT CCAAACAGGA CTGGGACAAG TACTGCCACT ACGTTGCTGG GCTGGTGGGA 
ATTGGCCTTT CTCGTCTATT CTCTGCCTCA GAGTTTGAAG ACCCCATAGT TGGTGAAGAC 
ATAGAGTGTG CCAACTCAAT GGGTCTGTTC CTGCAGAAAA CAAATATCAT TCGTGATTAT 
CTGGAAGACC AACAGGAAGG AAGGAAGTTT TGGCCTCAGG AGGTGTGGGG CAGATACATT 
AAGAAGTTGG AAGACTTTGC TAAGCCAGAG AACGTAGATG TGGCCGTGCA GTGCTTGAAT 
GAACTCATAA CCAACACCCT ACAGCACATC CCTGACGTCC TCACCTACCT GTCAAGGCTC 
CGGAACCAGA GTGTGTTTAA CTTCTGTGCT ATTCCACAGG TAATGGCCAT TGCCACACTG 
GCTGCCTGTT ACAATAACCA GCAGGTATTC AAAGGAGTAG TGAAGATTCG GAAGGGGCAA 
GCAGTCACCC TCATGATGGA TGCCACCAAC ATGCCTGCCG TCAAAGCTAT CATATACCAG 
TACATAGAAG AGATTTATCA CCGGATCCCC AACTCAGACC CATCATCAAG CAAAACCAAG 
CAGGTCATCT CCAAGATCAG GACACAGAAC CTTCCCAACT GCCAGCTCAT CTCCCGAAGC 
CACTACTCCC CCATTTACCT GTCATTTATC ATGCTCTTGG CTGCCCTGAG CTGGCAGTAC 
CTGAGCACCC TGTCCCAGGT CACAGAAGAC TATGTCCAGA GAGAACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034321.2
CDS	848..2098
Translation	MEFVKCLGHPEEFYNLLRFRMGGRRNFIPKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGDIRHAICVFYLVLRALDTVEDDMSISVEKKIPLLCNFHTFLYDPEWRFTESKEKDRQVLEDFPTISLEFRNLAEKYQTVIDDICHRMGCGMAEFVDKDVTSKQDWDKYCHYVAGLVGIGLSRLFSASEFEDPIVGEDIECANSMGLFLQKTNIIRDYLEDQQEGRKFWPQEVWGRYIKKLEDFAKPENVDVAVQCLNELITNTLQHIPDVLTYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIYQYIEEIYHRIPNSDPSSSKTKQVISKIRTQNLPNCQLISRSHYSPIYLSFIMLLAALSWQYLSTLSQVTEDYVQREH

Target ORF information:

RefSeq Version	NM_010191.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010191.3

ORF Insert Sequence:

ATGGAGTTCG TCAAGTGTCT AGGCCACCCG GAGGAGTTCT ATAACCTGCT GCGATTCCGC 
ATGGGAGGCC GGCGGAATTT TATACCCAAG ATGGACCAGG ACTCACTCAG CAGCAGCTTG 
AAGACCTGCT ACAAGTATCT CAATCAGACC AGTCGCAGCT TTGCCGCGGT TATCCAGGCG 
CTGGATGGGG ACATACGGCA CGCCATATGT GTGTTCTACC TGGTTCTCCG AGCCCTGGAT 
ACAGTGGAGG ATGACATGAG CATCAGTGTG GAGAAGAAGA TCCCACTGCT GTGTAACTTC 
CACACTTTCC TCTATGACCC AGAGTGGCGG TTCACTGAGA GCAAGGAGAA GGACCGACAA 
GTGCTGGAGG ACTTCCCCAC GATCTCCCTG GAGTTTAGAA ATTTGGCTGA GAAATATCAA 
ACAGTGATCG ATGACATCTG CCACCGGATG GGGTGTGGGA TGGCAGAATT TGTAGACAAG 
GATGTGACCT CCAAACAGGA CTGGGACAAG TACTGCCACT ACGTTGCTGG GCTGGTGGGA 
ATTGGCCTTT CTCGTCTATT CTCTGCCTCA GAGTTTGAAG ACCCCATAGT TGGTGAAGAC 
ATAGAGTGTG CCAACTCAAT GGGTCTGTTC CTGCAGAAAA CAAATATCAT TCGTGATTAT 
CTGGAAGACC AACAGGAAGG AAGGAAGTTT TGGCCTCAGG AGGTGTGGGG CAGATACATT 
AAGAAGTTGG AAGACTTTGC TAAGCCAGAG AACGTAGATG TGGCCGTGCA GTGCTTGAAT 
GAACTCATAA CCAACACCCT ACAGCACATC CCTGACGTCC TCACCTACCT GTCAAGGCTC 
CGGAACCAGA GTGTGTTTAA CTTCTGTGCT ATTCCACAGG TAATGGCCAT TGCCACACTG 
GCTGCCTGTT ACAATAACCA GCAGGTATTC AAAGGAGTAG TGAAGATTCG GAAGGGGCAA 
GCAGTCACCC TCATGATGGA TGCCACCAAC ATGCCTGCCG TCAAAGCTAT CATATACCAG 
TACATAGAAG AGATTTATCA CCGGATCCCC AACTCAGACC CATCATCAAG CAAAACCAAG 
CAGGTCATCT CCAAGATCAG GACACAGAAC CTTCCCAACT GCCAGCTCAT CTCCCGAAGC 
CACTACTCCC CCATTTACCT GTCATTTATC ATGCTCTTGG CTGCCCTGAG CTGGCAGTAC 
CTGAGCACCC TGTCCCAGGT CACAGAAGAC TATGTCCAGA GAGAACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fdft1 ( NM_001360211.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347140.1 Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05703
Clone ID Related Accession (Same CDS sequence)	NM_010191.3 , NM_001360211.1 , XM_017315844.1
Accession Version	NM_001360211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Mus musculus(house mouse)
Product	squalene synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY293142.1, AK169317.1 and BC054722.1. Transcript Variant: This variant (1) represents transcription from a major CAGE transcription cluster, and represents the shorter transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054722.1, BC138302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTCG TCAAGTGTCT AGGCCACCCG GAGGAGTTCT ATAACCTGCT GCGATTCCGC 
ATGGGAGGCC GGCGGAATTT TATACCCAAG ATGGACCAGG ACTCACTCAG CAGCAGCTTG 
AAGACCTGCT ACAAGTATCT CAATCAGACC AGTCGCAGCT TTGCCGCGGT TATCCAGGCG 
CTGGATGGGG ACATACGGCA CGCCATATGT GTGTTCTACC TGGTTCTCCG AGCCCTGGAT 
ACAGTGGAGG ATGACATGAG CATCAGTGTG GAGAAGAAGA TCCCACTGCT GTGTAACTTC 
CACACTTTCC TCTATGACCC AGAGTGGCGG TTCACTGAGA GCAAGGAGAA GGACCGACAA 
GTGCTGGAGG ACTTCCCCAC GATCTCCCTG GAGTTTAGAA ATTTGGCTGA GAAATATCAA 
ACAGTGATCG ATGACATCTG CCACCGGATG GGGTGTGGGA TGGCAGAATT TGTAGACAAG 
GATGTGACCT CCAAACAGGA CTGGGACAAG TACTGCCACT ACGTTGCTGG GCTGGTGGGA 
ATTGGCCTTT CTCGTCTATT CTCTGCCTCA GAGTTTGAAG ACCCCATAGT TGGTGAAGAC 
ATAGAGTGTG CCAACTCAAT GGGTCTGTTC CTGCAGAAAA CAAATATCAT TCGTGATTAT 
CTGGAAGACC AACAGGAAGG AAGGAAGTTT TGGCCTCAGG AGGTGTGGGG CAGATACATT 
AAGAAGTTGG AAGACTTTGC TAAGCCAGAG AACGTAGATG TGGCCGTGCA GTGCTTGAAT 
GAACTCATAA CCAACACCCT ACAGCACATC CCTGACGTCC TCACCTACCT GTCAAGGCTC 
CGGAACCAGA GTGTGTTTAA CTTCTGTGCT ATTCCACAGG TAATGGCCAT TGCCACACTG 
GCTGCCTGTT ACAATAACCA GCAGGTATTC AAAGGAGTAG TGAAGATTCG GAAGGGGCAA 
GCAGTCACCC TCATGATGGA TGCCACCAAC ATGCCTGCCG TCAAAGCTAT CATATACCAG 
TACATAGAAG AGATTTATCA CCGGATCCCC AACTCAGACC CATCATCAAG CAAAACCAAG 
CAGGTCATCT CCAAGATCAG GACACAGAAC CTTCCCAACT GCCAGCTCAT CTCCCGAAGC 
CACTACTCCC CCATTTACCT GTCATTTATC ATGCTCTTGG CTGCCCTGAG CTGGCAGTAC 
CTGAGCACCC TGTCCCAGGT CACAGAAGAC TATGTCCAGA GAGAACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347140.1
CDS	107..1357
Translation	MEFVKCLGHPEEFYNLLRFRMGGRRNFIPKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGDIRHAICVFYLVLRALDTVEDDMSISVEKKIPLLCNFHTFLYDPEWRFTESKEKDRQVLEDFPTISLEFRNLAEKYQTVIDDICHRMGCGMAEFVDKDVTSKQDWDKYCHYVAGLVGIGLSRLFSASEFEDPIVGEDIECANSMGLFLQKTNIIRDYLEDQQEGRKFWPQEVWGRYIKKLEDFAKPENVDVAVQCLNELITNTLQHIPDVLTYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIYQYIEEIYHRIPNSDPSSSKTKQVISKIRTQNLPNCQLISRSHYSPIYLSFIMLLAALSWQYLSTLSQVTEDYVQREH

Target ORF information:

RefSeq Version	NM_001360211.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360211.1

ORF Insert Sequence:

ATGGAGTTCG TCAAGTGTCT AGGCCACCCG GAGGAGTTCT ATAACCTGCT GCGATTCCGC 
ATGGGAGGCC GGCGGAATTT TATACCCAAG ATGGACCAGG ACTCACTCAG CAGCAGCTTG 
AAGACCTGCT ACAAGTATCT CAATCAGACC AGTCGCAGCT TTGCCGCGGT TATCCAGGCG 
CTGGATGGGG ACATACGGCA CGCCATATGT GTGTTCTACC TGGTTCTCCG AGCCCTGGAT 
ACAGTGGAGG ATGACATGAG CATCAGTGTG GAGAAGAAGA TCCCACTGCT GTGTAACTTC 
CACACTTTCC TCTATGACCC AGAGTGGCGG TTCACTGAGA GCAAGGAGAA GGACCGACAA 
GTGCTGGAGG ACTTCCCCAC GATCTCCCTG GAGTTTAGAA ATTTGGCTGA GAAATATCAA 
ACAGTGATCG ATGACATCTG CCACCGGATG GGGTGTGGGA TGGCAGAATT TGTAGACAAG 
GATGTGACCT CCAAACAGGA CTGGGACAAG TACTGCCACT ACGTTGCTGG GCTGGTGGGA 
ATTGGCCTTT CTCGTCTATT CTCTGCCTCA GAGTTTGAAG ACCCCATAGT TGGTGAAGAC 
ATAGAGTGTG CCAACTCAAT GGGTCTGTTC CTGCAGAAAA CAAATATCAT TCGTGATTAT 
CTGGAAGACC AACAGGAAGG AAGGAAGTTT TGGCCTCAGG AGGTGTGGGG CAGATACATT 
AAGAAGTTGG AAGACTTTGC TAAGCCAGAG AACGTAGATG TGGCCGTGCA GTGCTTGAAT 
GAACTCATAA CCAACACCCT ACAGCACATC CCTGACGTCC TCACCTACCT GTCAAGGCTC 
CGGAACCAGA GTGTGTTTAA CTTCTGTGCT ATTCCACAGG TAATGGCCAT TGCCACACTG 
GCTGCCTGTT ACAATAACCA GCAGGTATTC AAAGGAGTAG TGAAGATTCG GAAGGGGCAA 
GCAGTCACCC TCATGATGGA TGCCACCAAC ATGCCTGCCG TCAAAGCTAT CATATACCAG 
TACATAGAAG AGATTTATCA CCGGATCCCC AACTCAGACC CATCATCAAG CAAAACCAAG 
CAGGTCATCT CCAAGATCAG GACACAGAAC CTTCCCAACT GCCAGCTCAT CTCCCGAAGC 
CACTACTCCC CCATTTACCT GTCATTTATC ATGCTCTTGG CTGCCCTGAG CTGGCAGTAC 
CTGAGCACCC TGTCCCAGGT CACAGAAGAC TATGTCCAGA GAGAACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fdft1 ( XM_017315844.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171333.1 Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X1, mRNA.

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CloneID	OMu05703
Clone ID Related Accession (Same CDS sequence)	NM_010191.3 , NM_001360211.1 , XM_017315844.1
Accession Version	XM_017315844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	squalene synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTCG TCAAGTGTCT AGGCCACCCG GAGGAGTTCT ATAACCTGCT GCGATTCCGC 
ATGGGAGGCC GGCGGAATTT TATACCCAAG ATGGACCAGG ACTCACTCAG CAGCAGCTTG 
AAGACCTGCT ACAAGTATCT CAATCAGACC AGTCGCAGCT TTGCCGCGGT TATCCAGGCG 
CTGGATGGGG ACATACGGCA CGCCATATGT GTGTTCTACC TGGTTCTCCG AGCCCTGGAT 
ACAGTGGAGG ATGACATGAG CATCAGTGTG GAGAAGAAGA TCCCACTGCT GTGTAACTTC 
CACACTTTCC TCTATGACCC AGAGTGGCGG TTCACTGAGA GCAAGGAGAA GGACCGACAA 
GTGCTGGAGG ACTTCCCCAC GATCTCCCTG GAGTTTAGAA ATTTGGCTGA GAAATATCAA 
ACAGTGATCG ATGACATCTG CCACCGGATG GGGTGTGGGA TGGCAGAATT TGTAGACAAG 
GATGTGACCT CCAAACAGGA CTGGGACAAG TACTGCCACT ACGTTGCTGG GCTGGTGGGA 
ATTGGCCTTT CTCGTCTATT CTCTGCCTCA GAGTTTGAAG ACCCCATAGT TGGTGAAGAC 
ATAGAGTGTG CCAACTCAAT GGGTCTGTTC CTGCAGAAAA CAAATATCAT TCGTGATTAT 
CTGGAAGACC AACAGGAAGG AAGGAAGTTT TGGCCTCAGG AGGTGTGGGG CAGATACATT 
AAGAAGTTGG AAGACTTTGC TAAGCCAGAG AACGTAGATG TGGCCGTGCA GTGCTTGAAT 
GAACTCATAA CCAACACCCT ACAGCACATC CCTGACGTCC TCACCTACCT GTCAAGGCTC 
CGGAACCAGA GTGTGTTTAA CTTCTGTGCT ATTCCACAGG TAATGGCCAT TGCCACACTG 
GCTGCCTGTT ACAATAACCA GCAGGTATTC AAAGGAGTAG TGAAGATTCG GAAGGGGCAA 
GCAGTCACCC TCATGATGGA TGCCACCAAC ATGCCTGCCG TCAAAGCTAT CATATACCAG 
TACATAGAAG AGATTTATCA CCGGATCCCC AACTCAGACC CATCATCAAG CAAAACCAAG 
CAGGTCATCT CCAAGATCAG GACACAGAAC CTTCCCAACT GCCAGCTCAT CTCCCGAAGC 
CACTACTCCC CCATTTACCT GTCATTTATC ATGCTCTTGG CTGCCCTGAG CTGGCAGTAC 
CTGAGCACCC TGTCCCAGGT CACAGAAGAC TATGTCCAGA GAGAACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171333.1
CDS	3506..4756
Translation	MEFVKCLGHPEEFYNLLRFRMGGRRNFIPKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGDIRHAICVFYLVLRALDTVEDDMSISVEKKIPLLCNFHTFLYDPEWRFTESKEKDRQVLEDFPTISLEFRNLAEKYQTVIDDICHRMGCGMAEFVDKDVTSKQDWDKYCHYVAGLVGIGLSRLFSASEFEDPIVGEDIECANSMGLFLQKTNIIRDYLEDQQEGRKFWPQEVWGRYIKKLEDFAKPENVDVAVQCLNELITNTLQHIPDVLTYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIYQYIEEIYHRIPNSDPSSSKTKQVISKIRTQNLPNCQLISRSHYSPIYLSFIMLLAALSWQYLSTLSQVTEDYVQREH

Target ORF information:

RefSeq Version	XM_017315844.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus farnesyl diphosphate farnesyl transferase 1 (Fdft1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315844.1

ORF Insert Sequence:

ATGGAGTTCG TCAAGTGTCT AGGCCACCCG GAGGAGTTCT ATAACCTGCT GCGATTCCGC 
ATGGGAGGCC GGCGGAATTT TATACCCAAG ATGGACCAGG ACTCACTCAG CAGCAGCTTG 
AAGACCTGCT ACAAGTATCT CAATCAGACC AGTCGCAGCT TTGCCGCGGT TATCCAGGCG 
CTGGATGGGG ACATACGGCA CGCCATATGT GTGTTCTACC TGGTTCTCCG AGCCCTGGAT 
ACAGTGGAGG ATGACATGAG CATCAGTGTG GAGAAGAAGA TCCCACTGCT GTGTAACTTC 
CACACTTTCC TCTATGACCC AGAGTGGCGG TTCACTGAGA GCAAGGAGAA GGACCGACAA 
GTGCTGGAGG ACTTCCCCAC GATCTCCCTG GAGTTTAGAA ATTTGGCTGA GAAATATCAA 
ACAGTGATCG ATGACATCTG CCACCGGATG GGGTGTGGGA TGGCAGAATT TGTAGACAAG 
GATGTGACCT CCAAACAGGA CTGGGACAAG TACTGCCACT ACGTTGCTGG GCTGGTGGGA 
ATTGGCCTTT CTCGTCTATT CTCTGCCTCA GAGTTTGAAG ACCCCATAGT TGGTGAAGAC 
ATAGAGTGTG CCAACTCAAT GGGTCTGTTC CTGCAGAAAA CAAATATCAT TCGTGATTAT 
CTGGAAGACC AACAGGAAGG AAGGAAGTTT TGGCCTCAGG AGGTGTGGGG CAGATACATT 
AAGAAGTTGG AAGACTTTGC TAAGCCAGAG AACGTAGATG TGGCCGTGCA GTGCTTGAAT 
GAACTCATAA CCAACACCCT ACAGCACATC CCTGACGTCC TCACCTACCT GTCAAGGCTC 
CGGAACCAGA GTGTGTTTAA CTTCTGTGCT ATTCCACAGG TAATGGCCAT TGCCACACTG 
GCTGCCTGTT ACAATAACCA GCAGGTATTC AAAGGAGTAG TGAAGATTCG GAAGGGGCAA 
GCAGTCACCC TCATGATGGA TGCCACCAAC ATGCCTGCCG TCAAAGCTAT CATATACCAG 
TACATAGAAG AGATTTATCA CCGGATCCCC AACTCAGACC CATCATCAAG CAAAACCAAG 
CAGGTCATCT CCAAGATCAG GACACAGAAC CTTCCCAACT GCCAGCTCAT CTCCCGAAGC 
CACTACTCCC CCATTTACCT GTCATTTATC ATGCTCTTGG CTGCCCTGAG CTGGCAGTAC 
CTGAGCACCC TGTCCCAGGT CACAGAAGAC TATGTCCAGA GAGAACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maf links Neuregulin1 signaling to cholesterol synthesis in myelinating Schwann cells.
Genes & development32(9-10)645-657(2018 05)
Kim M,Wende H,Walcher J,Kühnemund J,Cheret C,Kempa S,McShane E,Selbach M,Lewin GR,Birchmeier C

Cytoplasmic cleavage of DPPA3 is required for intracellular trafficking and cleavage-stage development in mice.
Nature communications8(1)1643(2017 11)
Shin SW,Vogt EJ,Jimenez-Movilla M,Baibakov B,Dean J

Mfsd2a Is a Transporter for the Essential ω-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.
The Journal of biological chemistry291(20)10501-14(2016 May)
Wong BH,Chan JP,Cazenave-Gassiot A,Poh RW,Foo JC,Galam DL,Ghosh S,Nguyen LN,Barathi VA,Yeo SW,Luu CD,Wenk MR,Silver DL

A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.
Journal of lipid research56(8)1551-9(2015 Aug)
Şişecioğlu M,Budak H,Geffers L,Çankaya M,Çiftci M,Thaller C,Eichele G,Küfrevioğlu Öİ,Özdemir H

Plasma cholesterol-lowering and transient liver dysfunction in mice lacking squalene synthase in the liver.
Journal of lipid research56(5)998-1005(2015 May)
Nagashima S,Yagyu H,Tozawa R,Tazoe F,Takahashi M,Kitamine T,Yamamuro D,Sakai K,Sekiya M,Okazaki H,Osuga J,Honda A,Ishibashi S

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Fdft1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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