F7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	F7
Entrez Gene ID	14068
Full Name	coagulation factor VII
Synonyms	AI132620,Cf7,FVII
General protein information	Preferred Names coagulation factor VII Names coagulation factor VII serum prothrombin conversion accelerator
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 5.73 cM
Summary	This gene encodes a vitamin K-dependent serine protease that plays a critical role in the extrinsic pathway of blood coagulation. Upon contact with tissue factor III (TF III), the encoded protein forms an activated complex termed TF-FVIIa that initiates the coagulation cascade involving other coagulation factors, ultimately resulting in a fibrin clot. Complete lack of the encoded protein in mice results in in perinatal lethality due to bleeding from normal blood vessels. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312562.1	XP_017168051.1	coagulation factor VII isoform X1
NM_010172.4	NP_034302.2	coagulation factor VII preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	F7	NP_001029978.1
Rattus norvegicus (Norway rat)	F7	NP_690059.1
Mus musculus (house mouse)	F7	NP_034302.2
Macaca mulatta (Rhesus monkey)	F7	NP_001073605.1
Gallus gallus (chicken)	F7	NP_989773.1
Homo sapiens (human)	F7	NP_062562.1
Pan troglodytes (chimpanzee)	F7	XP_001149885.2
Canis lupus familiaris (dog)	F7	NP_001041498.1
Xenopus tropicalis (tropical clawed frog)	f7	NP_001072819.1
Danio rerio (zebrafish)	zgc:163025	NP_001083027.1

Gene Ontology
Bibliography

Related articles in PubMed

Glial cells maintain synapses by inhibiting an activity-dependent retrograde protease signal.
Gould TW, Dominguez B, de Winter F, Yeo GW, Liu P, Sundararaman B, Stark T, Vu A, Degen JL, Lin W, Lee KF
PLoS genetics15(3)e1007948(2019 03)

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.
Lacombe J, Rishavy MA, Berkner KL, Ferron M
JCI insight3(1)(2018 01)

One amino acid in mouse activated factor VII defines its endothelial protein C receptor (EPCR) binding and modulates its EPCR-dependent hemostatic activity in vivo.
Pavani G, Zintner SM, Ivanciu L, Small JC, Stafford KA, Szeto JH, Margaritis P
Journal of thrombosis and haemostasis : JTH15(3)507-512(2017 03)

LDL receptor-related protein 1 contributes to the clearance of the activated factor VII-antithrombin complex.
Fazavana JG, Muczynski V, Proulle V, Wohner N, Christophe OD, Lenting PJ, Denis CV
Journal of thrombosis and haemostasis : JTH14(12)2458-2470(2016 12)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu82346	XM_017312562.1 Latest version!	Mus musculus coagulation factor VII (F7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu22920	NM_010172.4 Latest version!	Mus musculus coagulation factor VII (F7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F7 ( XM_017312562.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168051.1 Mus musculus coagulation factor VII (F7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82346
Clone ID Related Accession (Same CDS sequence)	XM_017312562.1
Accession Version	XM_017312562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	coagulation factor VII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCAG CCAATGCTCC AGCTTTCTCC ACCCCTCTCC CCTCCCCCCT GACCTTTGCC 
CTCCCGGCAG CATCTGGACA GTCACCAGCC AGAAGCCACA GTCTCATCAT GGTTCCACAG 
GCGCATGGGC TGCTGCTTCT CTGCTTTCTG CTCCAGCTCC AGGGACCTCT AGGGACTGCA 
GTTTTCATAA CCCAGGAGGA AGCACATGGT GTCCTACACA GGCAAAGGCG TGCCAACTCA 
CTCCTGGAGG AGCTTTGGCC CGGCTCTCTG GAGAGAGAGT GCAATGAGGA ACAGTGCTCC 
TTTGAGGAGG CCCGGGAGAT CTTCAAGAGC CCTGAGAGGA CCAAGCAGTT CTGGATTGTT 
TACAGTGATG GGGACCAGTG TGCCTCGAAT CCATGTCAGA ACGGAGGTAC CTGCCAGGAT 
CATCTCAAGT CTTACGTCTG CTTCTGCCTC CTAGACTTTG AGGGTCGGAA CTGTGAGAAA 
AGCAAGAATG AGCAGCTGAT CTGTGCAAAT GAAAATGGTG ACTGTGACCA GTACTGCAGG 
GACCATGTAG GGACCAAGCG TACCTGTAGC TGTCATGAGG ACTACACGCT ACAGCCAGAT 
GAGGTGTCCT GCAAACCAAA AGCCTTCGCA GTGGGTGGCG CCCGAGTCTG CCTTAAGGAC 
CCTGTCTTCC TGTGCTGGTG GCCCATGCTT ACACAGATCG TCACTTTCTT CATAGCGAAC 
CAGAACGACA CGTTCTCCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168051.1
CDS	446..1189
Translation	MLPANAPAFSTPLPSPLTFALPAASGQSPARSHSLIMVPQAHGLLLLCFLLQLQGPLGTAVFITQEEAHGVLHRQRRANSLLEELWPGSLERECNEEQCSFEEAREIFKSPERTKQFWIVYSDGDQCASNPCQNGGTCQDHLKSYVCFCLLDFEGRNCEKSKNEQLICANENGDCDQYCRDHVGTKRTCSCHEDYTLQPDEVSCKPKAFAVGGARVCLKDPVFLCWWPMLTQIVTFFIANQNDTFSL

Target ORF information:

RefSeq Version	XM_017312562.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor VII (F7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312562.1

ORF Insert Sequence:

ATGCTTCCAG CCAATGCTCC AGCTTTCTCC ACCCCTCTCC CCTCCCCCCT GACCTTTGCC 
CTCCCGGCAG CATCTGGACA GTCACCAGCC AGAAGCCACA GTCTCATCAT GGTTCCACAG 
GCGCATGGGC TGCTGCTTCT CTGCTTTCTG CTCCAGCTCC AGGGACCTCT AGGGACTGCA 
GTTTTCATAA CCCAGGAGGA AGCACATGGT GTCCTACACA GGCAAAGGCG TGCCAACTCA 
CTCCTGGAGG AGCTTTGGCC CGGCTCTCTG GAGAGAGAGT GCAATGAGGA ACAGTGCTCC 
TTTGAGGAGG CCCGGGAGAT CTTCAAGAGC CCTGAGAGGA CCAAGCAGTT CTGGATTGTT 
TACAGTGATG GGGACCAGTG TGCCTCGAAT CCATGTCAGA ACGGAGGTAC CTGCCAGGAT 
CATCTCAAGT CTTACGTCTG CTTCTGCCTC CTAGACTTTG AGGGTCGGAA CTGTGAGAAA 
AGCAAGAATG AGCAGCTGAT CTGTGCAAAT GAAAATGGTG ACTGTGACCA GTACTGCAGG 
GACCATGTAG GGACCAAGCG TACCTGTAGC TGTCATGAGG ACTACACGCT ACAGCCAGAT 
GAGGTGTCCT GCAAACCAAA AGCCTTCGCA GTGGGTGGCG CCCGAGTCTG CCTTAAGGAC 
CCTGTCTTCC TGTGCTGGTG GCCCATGCTT ACACAGATCG TCACTTTCTT CATAGCGAAC 
CAGAACGACA CGTTCTCCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F7 ( NM_010172.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034302.2 Mus musculus coagulation factor VII (F7), mRNA.

Price & Availability↑

CloneID	OMu22920
Clone ID Related Accession (Same CDS sequence)	NM_010172.4
Accession Version	NM_010172.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Mus musculus(house mouse)
Product	coagulation factor VII preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC127308.3, AK171362.1 and BC061149.1. On May 21, 2015 this sequence version replaced NM_010172.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061149.1, AK171362.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCCAC AGGCGCATGG GCTGCTGCTT CTCTGCTTTC TGCTCCAGCT CCAGGGACCT 
CTAGGGACTG CAGTTTTCAT AACCCAGGAG GAAGCACATG GTGTCCTACA CAGGCAAAGG 
CGTGCCAACT CACTCCTGGA GGAGCTTTGG CCCGGCTCTC TGGAGAGAGA GTGCAATGAG 
GAACAGTGCT CCTTTGAGGA GGCCCGGGAG ATCTTCAAGA GCCCTGAGAG GACCAAGCAG 
TTCTGGATTG TTTACAGTGA TGGGGACCAG TGTGCCTCGA ATCCATGTCA GAACGGAGGT 
ACCTGCCAGG ATCATCTCAA GTCTTACGTC TGCTTCTGCC TCCTAGACTT TGAGGGTCGG 
AACTGTGAGA AAAGCAAGAA TGAGCAGCTG ATCTGTGCAA ATGAAAATGG TGACTGTGAC 
CAGTACTGCA GGGACCATGT AGGGACCAAG CGTACCTGTA GCTGTCATGA GGACTACACG 
CTACAGCCAG ATGAGGTGTC CTGCAAACCA AAAGTTGAGT ACCCGTGTGG GAGAATACCT 
GTTGTAGAAA AAAGAAACTC CAGCAGCCGC CAAGGCCGCA TTGTGGGAGG CAACGTGTGC 
CCCAAAGGGG AGTGTCCATG GCAGGCTGTG CTGAAAATCA ATGGGTTATT GCTGTGTGGG 
GCCGTCCTGC TGGACGCCAG ATGGATAGTG ACCGCAGCCC ACTGCTTCGA TAATATCCGC 
TACTGGGGAA ACATCACAGT GGTGATGGGT GAACATGACT TCAGTGAGAA GGATGGGGAT 
GAGCAAGTAC GACGGGTGAC ACAGGTCATC ATGCCCGACA AGTACATCCG CGGCAAGATC 
AACCACGACA TTGCCCTGCT CCGCCTTCAC CGGCCTGTGA CCTTCACTGA CTACGTGGTG 
CCCCTGTGTC TGCCTGAAAA GTCCTTCTCC GAGAACACCC TAGCCAGAAT CCGCTTCTCA 
AGGGTCAGTG GCTGGGGCCA GCTACTGGAC CGTGGTGCCA CAGCCCTGGA ACTCATGTCC 
ATCGAGGTGC CCCGGCTGAT GACCCAGGAC TGTCTGGAGC ACGCCAAGCA CAGCTCTAAC 
ACCCCCAAAA TCACAGAGAA CATGTTCTGC GCTGGCTACA TGGATGGTAC CAAGGACGCC 
TGCAAGGGTG ACAGCGGTGG CCCACATGCC ACGCACTACC ATGGCACATG GTATCTGACA 
GGTGTGGTCA GCTGGGGGGA GGGCTGTGCA GCTATTGGTC ACATTGGGGT GTACACCAGG 
GTCTCCCAGT ACATAGACTG GCTGGTCAGA CACATGGACT CCAAGCTCCA GGTTGGGGTT 
TTCCGACTCC CACTACTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034302.2
CDS	26..1366
Translation	MVPQAHGLLLLCFLLQLQGPLGTAVFITQEEAHGVLHRQRRANSLLEELWPGSLERECNEEQCSFEEAREIFKSPERTKQFWIVYSDGDQCASNPCQNGGTCQDHLKSYVCFCLLDFEGRNCEKSKNEQLICANENGDCDQYCRDHVGTKRTCSCHEDYTLQPDEVSCKPKVEYPCGRIPVVEKRNSSSRQGRIVGGNVCPKGECPWQAVLKINGLLLCGAVLLDARWIVTAAHCFDNIRYWGNITVVMGEHDFSEKDGDEQVRRVTQVIMPDKYIRGKINHDIALLRLHRPVTFTDYVVPLCLPEKSFSENTLARIRFSRVSGWGQLLDRGATALELMSIEVPRLMTQDCLEHAKHSSNTPKITENMFCAGYMDGTKDACKGDSGGPHATHYHGTWYLTGVVSWGEGCAAIGHIGVYTRVSQYIDWLVRHMDSKLQVGVFRLPLL

Target ORF information:

RefSeq Version	NM_010172.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus coagulation factor VII (F7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010172.4

ORF Insert Sequence:

ATGGTTCCAC AGGCGCATGG GCTGCTGCTT CTCTGCTTTC TGCTCCAGCT CCAGGGACCT 
CTAGGGACTG CAGTTTTCAT AACCCAGGAG GAAGCACATG GTGTCCTACA CAGGCAAAGG 
CGTGCCAACT CACTCCTGGA GGAGCTTTGG CCCGGCTCTC TGGAGAGAGA GTGCAATGAG 
GAACAGTGCT CCTTTGAGGA GGCCCGGGAG ATCTTCAAGA GCCCTGAGAG GACCAAGCAG 
TTCTGGATTG TTTACAGTGA TGGGGACCAG TGTGCCTCGA ATCCATGTCA GAACGGAGGT 
ACCTGCCAGG ATCATCTCAA GTCTTACGTC TGCTTCTGCC TCCTAGACTT TGAGGGTCGG 
AACTGTGAGA AAAGCAAGAA TGAGCAGCTG ATCTGTGCAA ATGAAAATGG TGACTGTGAC 
CAGTACTGCA GGGACCATGT AGGGACCAAG CGTACCTGTA GCTGTCATGA GGACTACACG 
CTACAGCCAG ATGAGGTGTC CTGCAAACCA AAAGTTGAGT ACCCGTGTGG GAGAATACCT 
GTTGTAGAAA AAAGAAACTC CAGCAGCCGC CAAGGCCGCA TTGTGGGAGG CAACGTGTGC 
CCCAAAGGGG AGTGTCCATG GCAGGCTGTG CTGAAAATCA ATGGGTTATT GCTGTGTGGG 
GCCGTCCTGC TGGACGCCAG ATGGATAGTG ACCGCAGCCC ACTGCTTCGA TAATATCCGC 
TACTGGGGAA ACATCACAGT GGTGATGGGT GAACATGACT TCAGTGAGAA GGATGGGGAT 
GAGCAAGTAC GACGGGTGAC ACAGGTCATC ATGCCCGACA AGTACATCCG CGGCAAGATC 
AACCACGACA TTGCCCTGCT CCGCCTTCAC CGGCCTGTGA CCTTCACTGA CTACGTGGTG 
CCCCTGTGTC TGCCTGAAAA GTCCTTCTCC GAGAACACCC TAGCCAGAAT CCGCTTCTCA 
AGGGTCAGTG GCTGGGGCCA GCTACTGGAC CGTGGTGCCA CAGCCCTGGA ACTCATGTCC 
ATCGAGGTGC CCCGGCTGAT GACCCAGGAC TGTCTGGAGC ACGCCAAGCA CAGCTCTAAC 
ACCCCCAAAA TCACAGAGAA CATGTTCTGC GCTGGCTACA TGGATGGTAC CAAGGACGCC 
TGCAAGGGTG ACAGCGGTGG CCCACATGCC ACGCACTACC ATGGCACATG GTATCTGACA 
GGTGTGGTCA GCTGGGGGGA GGGCTGTGCA GCTATTGGTC ACATTGGGGT GTACACCAGG 
GTCTCCCAGT ACATAGACTG GCTGGTCAGA CACATGGACT CCAAGCTCCA GGTTGGGGTT 
TTCCGACTCC CACTACTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glial cells maintain synapses by inhibiting an activity-dependent retrograde protease signal.
PLoS genetics15(3)e1007948(2019 03)
Gould TW,Dominguez B,de Winter F,Yeo GW,Liu P,Sundararaman B,Stark T,Vu A,Degen JL,Lin W,Lee KF

VKOR paralog VKORC1L1 supports vitamin K-dependent protein carboxylation in vivo.
JCI insight3(1)(2018 01)
Lacombe J,Rishavy MA,Berkner KL,Ferron M

One amino acid in mouse activated factor VII defines its endothelial protein C receptor (EPCR) binding and modulates its EPCR-dependent hemostatic activity in vivo.
Journal of thrombosis and haemostasis : JTH15(3)507-512(2017 03)
Pavani G,Zintner SM,Ivanciu L,Small JC,Stafford KA,Szeto JH,Margaritis P

LDL receptor-related protein 1 contributes to the clearance of the activated factor VII-antithrombin complex.
Journal of thrombosis and haemostasis : JTH14(12)2458-2470(2016 12)
Fazavana JG,Muczynski V,Proulle V,Wohner N,Christophe OD,Lenting PJ,Denis CV

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

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F7 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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