| Gene Symbol | Dhfr |
| Entrez Gene ID | 13361 |
| Full Name | dihydrofolate reductase |
| Synonyms | 8430436I03Rik,AA607882,AI662710,AW555094 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome |
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Dhfr cDNA ORF clone, Mus musculus(house mouse)
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Gene
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Clones
gRNAs
The following Dhfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OMu15718 Clone ID Related Accession (Same CDS sequence) NM_010049.3 Accession Version NM_010049.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 564bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1577116800000 Organism Mus musculus(house mouse) Product dihydrofolate reductase Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU058996.1, AK031241.1, CB247191.1, CAAA01146114.1 and AI854085.1. On Jun 29, 2005 this sequence version replaced NM_010049.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK136212.1, AK031241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##
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541ATGGTTCGAC CATTGAACTG CATCGTCGCC GTGTCCCAAA ATATGGGGAT TGGCAAGAAC
GGAGACCTAC CCTGGCCTCC GCTCAGGAAC GAGTTCAAGT ACTTCCAAAG AATGACCACA
ACCTCTTCAG TGGAAGGTAA ACAGAATCTG GTGATTATGG GTAGGAAAAC CTGGTTCTCC
ATTCCTGAGA AGAATCGACC TTTAAAGGAC AGAATTAATA TAGTTCTCAG TAGAGAACTC
AAAGAACCAC CACGAGGAGC TCATTTTCTT GCCAAAAGTT TGGATGATGC CTTAAGACTT
ATTGAACAAC CGGAATTGGC AAGTAAAGTA GACATGGTTT GGATAGTCGG AGGCAGTTCT
GTTTACCAGG AAGCCATGAA TCAACCAGGC CACCTCAGAC TCTTTGTGAC AAGGATCATG
CAGGAATTTG AAAGTGACAC GTTTTTCCCA GAAATTGATT TGGGGAAATA TAAACTTCTC
CCAGAATACC CAGGCGTCCT CTCTGAGGTC CAGGAGGAAA AAGGCATCAA GTATAAGTTT
GAAGTCTACG AGAAGAAAGA CTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_034179.1 CDS 477..1040 Translation 
Target ORF information:
RefSeq Version NM_010049.3 Organism Mus musculus(house mouse) Definition Mus musculus dihydrofolate reductase (Dhfr), mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_010049.3
ORF Insert Sequence:
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541ATGGTTCGAC CATTGAACTG CATCGTCGCC GTGTCCCAAA ATATGGGGAT TGGCAAGAAC
GGAGACCTAC CCTGGCCTCC GCTCAGGAAC GAGTTCAAGT ACTTCCAAAG AATGACCACA
ACCTCTTCAG TGGAAGGTAA ACAGAATCTG GTGATTATGG GTAGGAAAAC CTGGTTCTCC
ATTCCTGAGA AGAATCGACC TTTAAAGGAC AGAATTAATA TAGTTCTCAG TAGAGAACTC
AAAGAACCAC CACGAGGAGC TCATTTTCTT GCCAAAAGTT TGGATGATGC CTTAAGACTT
ATTGAACAAC CGGAATTGGC AAGTAAAGTA GACATGGTTT GGATAGTCGG AGGCAGTTCT
GTTTACCAGG AAGCCATGAA TCAACCAGGC CACCTCAGAC TCTTTGTGAC AAGGATCATG
CAGGAATTTG AAAGTGACAC GTTTTTCCCA GAAATTGATT TGGGGAAATA TAAACTTCTC
CCAGAATACC CAGGCGTCCT CTCTGAGGTC CAGGAGGAAA AAGGCATCAA GTATAAGTTT
GAAGTCTACG AGAAGAAAGA CTAAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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PubMed
Knockout of dihydrofolate reductase in mice induces hypertension and abdominal aortic aneurysm via mitochondrial dysfunction.
Redox biology24101185(2019 06)
Li Q,Youn JY,Siu KL,Murugesan P,Zhang Y,Cai H
Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation.
Cell reports23(10)2864-2873.e7(2018 06)
Fawal MA,Jungas T,Kischel A,Audouard C,Iacovoni JS,Davy A
Ectopic expression of Hoxb1 induces cardiac and craniofacial malformations.
Genesis (New York, N.Y. : 2000)56(6-7)e23221(2018 06)
Zaffran S,Odelin G,Stefanovic S,Lescroart F,Etchevers HC
High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA
Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr.
Molecular and cellular biology36(8)1222-36(2016 Apr)
Thoms JA,Knezevic K,Liu JJ,Glaros EN,Thai T,Qiao Q,Campbell H,Packham D,Huang Y,Papathanasiou P,Tunningley R,Whittle B,Yeung AW,Chandrakanthan V,Hesson L,Chen V,Wong JW,Purton LE,Ward RL,Thomas SR,Pimanda JE
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