Col1a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col1a2
Entrez Gene ID	12843
Full Name	collagen, type I, alpha 2
Synonyms	AA960264,AI325291,Col1a-2,Cola-2,Cola2,oim
General protein information	Preferred Names collagen, type I, alpha 2 Names collagen alpha-2(I) chain alpha-2 type I collagen collagen COL1A2 procollagen, type I, alpha 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 A1\|6 1.81 cM
Summary	This gene encodes the alpha-2 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-1 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice harboring certain mutations in the encoded gene exhibit symptoms of moderate to severe forms of osteogenesis imperfecta. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007743.3	NP_031769.2	collagen alpha-2(I) chain preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL1A2	XP_001168894.1
Xenopus tropicalis (tropical clawed frog)	col1a2	NP_001072718.1
Rattus norvegicus (Norway rat)	Col1a2	NP_445808.1
Homo sapiens (human)	COL1A2	NP_000080.2
Mus musculus (house mouse)	Col1a2	NP_031769.2
Gallus gallus (chicken)	COL1A2	NP_001073182.2
Macaca mulatta (Rhesus monkey)	COL1A2	XP_001097831.1
Canis lupus familiaris (dog)	COL1A2	NP_001003187.1
Bos taurus (cattle)	COL1A2	NP_776945.1
Danio rerio (zebrafish)	col1a2	NP_892013.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of flow responsive Tie1 results in Impaired Aortic valve remodeling.
Qu X, Violette K, Sewell-Loftin MK, Soslow J, Saint-Jean L, Hinton RB, Merryman WD, Baldwin HS
Developmental biology455(1)73-84(2019 Nov)

Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia.
Chao CM, Moiseenko A, Kosanovic D, Rivetti S, El Agha E, Wilhelm J, Kampschulte M, Yahya F, Ehrhardt H, Zimmer KP, Barreto G, Rizvanov AA, Schermuly RT, Reiss I, Morty RE, Rottier RJ, Bellusci S, Zhang JS
Human molecular genetics28(9)1429-1444(2019 05)

Transcriptome analysis of mouse and human sinoatrial node cells reveals a conserved genetic program.
van Eif VWW, Stefanovic S, van Duijvenboden K, Bakker M, Wakker V, de Gier-de Vries C, Zaffran S, Verkerk AO, Boukens BJ, Christoffels VM
Development (Cambridge, England)146(8)(2019 04)

Endoplasmic reticulum stress is induced in growth plate hypertrophic chondrocytes in G610C mouse model of osteogenesis imperfecta.
Scheiber AL, Guess AJ, Kaito T, Abzug JM, Enomoto-Iwamoto M, Leikin S, Iwamoto M, Otsuru S
Biochemical and biophysical research communications509(1)235-240(2019 01)

TBX2 and TBX3 act downstream of canonical WNT signaling in patterning and differentiation of the mouse ureteric mesenchyme.
Aydoğdu N, Rudat C, Trowe MO, Kaiser M, Lüdtke TH, Taketo MM, Christoffels VM, Moon A, Kispert A
Development (Cambridge, England)145(23)(2018 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Col1a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col1a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21753	NM_007743.3 Latest version!	Mus musculus collagen, type I, alpha 2 (Col1a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col1a2 ( NM_007743.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031769.2 Mus musculus collagen, type I, alpha 2 (Col1a2), mRNA.

Price & Availability↑

CloneID	OMu21753
Clone ID Related Accession (Same CDS sequence)	NM_007743.3
Accession Version	NM_007743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Mus musculus(house mouse)
Product	collagen alpha-2(I) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY138414.1, AK159212.1 and AK132496.1. On Dec 2, 2015 this sequence version replaced NM_007743.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132496.1, AK159578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCAGCT TTGTGGATAC GCGGACTCTG TTGCTGCTTG CAGTAACTTC GTGCCTAGCA 
ACATGCCAAT ATTTGCAATC GGGATCAGTA CGAAAGGGCC CCACTGGAGA CAGAGGACCA 
CGTGGACAAA GGGGTCCCGC AGGTCCCCGA GGCAGAGATG GTGTTGATGG TCCCATGGGC 
CCTCCTGGTC CCCCTGGCTC CCCTGGTCCT CCCGGCTCCC CTGCCCCCCC TGGTCTTACT 
GGGAACTTTG CTGCTCAGTA TTCTGACAAA GGAGTTTCAT CTGGCCCTGG ACCAATGGGT 
TTAATGGGAC CCAGAGGCCC TCCTGGTGCT GTTGGAGCCC CTGGCCCTCA AGGTTTCCAA 
GGACCTGCTG GTGAACCTGG TGAGCCTGGT CAAACGGGTC CTGCAGGTCC CCGTGGTCCA 
GCTGGCTCTC CTGGCAAGGC TGGTGAGGAT GGTCACCCTG GAAAACCCGG AAGACCTGGG 
GAGAGAGGAG TCGTTGGACC ACAGGGTGCT CGTGGTTTCC CCGGGACTCC TGGACTTCCT 
GGCTTCAAAG GCGTGAAAGG ACACAGTGGT ATGGATGGAT TAAAGGGACA GCCTGGTGCA 
CAGGGTGTGA AGGGTGAACC CGGTGCCCCT GGAGAGAATG GAACTCCAGG TCAAGCAGGA 
GCCCGAGGGC TTCCTGGTGA GAGAGGGCGT GTCGGAGCTC CTGGTCCAGC TGGTGCCCGA 
GGCAGTGATG GAAGCGTTGG CCCCGTCGGC CCTGCTGGTC CCATTGGGTC TGCTGGACCC 
CCTGGTTTCC CAGGTGCTCC TGGTCCCAAG GGTGAACTTG GGCCCGTTGG TAACCCTGGC 
CCTGCTGGTC CTGCTGGTCC CCGTGGTGAA GTGGGTCTTC CAGGTCTTTC TGGCCCTGTT 
GGACCTCCTG GCAACCCTGG AACAAATGGG CTCACTGGCG CCAAGGGTGC TACTGGACTC 
CCTGGTGTCG CTGGGGCTCC CGGTCTCCCT GGCCCCCGTG GTATTCCTGG CCCCGCTGGT 
GCTGCTGGTG CTACAGGTGC CAGAGGACTT GTTGGTGAGC CTGGTCCTGC TGGCTCCAAA 
GGAGAATCCG GTAACAAGGG TGAGCCTGGC TCTGTTGGAG CCCAAGGTCC TCCTGGTCCC 
AGCGGTGAAG AAGGAAAGAG AGGGTCTCCC GGAGAAGCTG GATCTGCTGG CCCTGCAGGG 
CCTCCAGGGC TTAGAGGCAG CCCTGGTTCT CGAGGTCTTC CTGGAGCTGA TGGCAGAGCT 
GGTGTAATGG GTCCTCCTGG CAATCGTGGT TCAACCGGCC CTGCTGGAAT CCGAGGTCCT 
AATGGAGATG CCGGTCGACC TGGGGAACCT GGTCTCATGG GACCCAGAGG TCTTCCTGGG 
TCTCCTGGAA ATGTTGGCCC ATCTGGTAAA GAAGGCCCTG TGGGTCTCCC TGGCATTGAT 
GGCAGACCTG GCCCAATCGG CCCAGCTGGA CCAAGAGGTG AAGCTGGCAA CATCGGATTC 
CCTGGACCCA AAGGCCCCTC TGGTGATCCT GGCAAACCTG GTGAGAGAGG ACACCCCGGT 
CTTGCTGGTG CTCGGGGAGC TCCAGGACCC GATGGCAACA ATGGAGCTCA GGGCCCCCCT 
GGACCCCAGG GTGTTCAAGG TGGCAAAGGT GAACAAGGCC CTGCTGGTCC TCCTGGCTTC 
CAGGGTCTCC CTGGTCCCTC AGGTACTACT GGAGAAGTTG GCAAGCCCGG AGAAAGGGGT 
CTTCCTGGTG AATTCGGTCT CCCTGGTCCT GCTGGTCCAA GAGGAGAACG TGGTACCCCG 
GGTGAGAGTG GAGCTGCTGG CCCTTCTGGT CCTATTGGAA GCCGAGGTCC CAGTGGAGCC 
CCAGGGCCTG ATGGAAACAA GGGTGAAGCT GGTGCAGTCG GTGCTCCAGG CAGTGCTGGT 
GCCTCTGGGC CTGGTGGGCT TCCAGGAGAG AGGGGTGCTG CTGGCATACC TGGGGGCAAA 
GGAGAAAAGG GTGAAACTGG TCTCCGAGGT GACACTGGCA ACACTGGTAG AGATGGTGCT 
CGTGGCATTC CTGGTGCTGT AGGTGCCCCT GGTCCTGCTG GGGCCTCAGG TGACCGGGGT 
GAAGCTGGTG CTGCCGGTCC TTCTGGCCCA GCTGGTCCTC GGGGTAGCCC TGGTGAACGT 
GGTGAAGTTG GCCCTGCTGG CCCCAATGGA TTTGCTGGTC CTGCTGGTGC TGCTGGCCAA 
CCCGGTGCTA AAGGAGAGAA GGGAACCAAA GGGCCTAAGG GTGAAAATGG CATTGTTGGT 
CCAACCGGGT CTGTTGGAGC TGCTGGCCCA TCTGGTCCTA ATGGCCCACC TGGTCCTGTT 
GGAAGTCGTG GTGATGGAGG GCCCCCTGGT ATGACTGGCT TCCCTGGTGC TGCTGGACGG 
ACTGGTCCCC CCGGACCCAG TGGTATTGCT GGCCCCCCTG GCCCCCCTGG TGCTGCTGGA 
AAGGAAGGGA TTCGTGGTCC CCGTGGCGAC CAAGGTCCAG TTGGCCGAAC TGGAGAAACA 
GGTGCATCTG GACCCCCTGG ATTTGTTGGT GAAAAGGGTC CCTCTGGAGA ACCTGGTACC 
GCAGGAGCCC CTGGTACCGC AGGTCCTCAG GGTCTTCTTG GTGCTCCCGG TATTCTGGGT 
CTCCCTGGCT CTAGAGGTGA ACGTGGTCTG CCAGGTATTG CTGGTGCTCT GGGTGAACCT 
GGTCCTCTGG GCATCTCAGG CCCTCCTGGT GCCCGTGGTC CCCCTGGTGC TGTGGGCAGC 
CCTGGAGTTA ATGGTGCCCC TGGTGAAGCT GGTCGTGATG GTAACCCTGG CAGCGATGGT 
CCCCCAGGTC GTGATGGTCA GCCTGGACAC AAGGGAGAGC GTGGTTACCC TGGCAGCATT 
GGTCCGACTG GTGCTGCAGG TGCCCCTGGT CCTCACGGTT CTGTGGGTCC TGCTGGGAAA 
CATGGAAACC GAGGTGAACC TGGTCCTGCC GGTTCTGTTG GTCCTGTTGG TGCTGTTGGT 
CCAAGAGGTC CTAGTGGTCC TCAAGGCATC CGAGGTGACA AAGGAGAACC TGGTGATAAA 
GGGCACAGGG GTCTTCCTGG CTTAAAAGGA TACAGTGGAT TGCAGGGTCT TCCTGGTCTT 
GCTGGCCTAC ATGGTGACCA AGGAGCTCCT GGTCCTGTGG GTCCTGCTGG TCCCAGGGGT 
CCTGCTGGGC CTTCTGGACC CGTTGGCAAA GATGGTAGAT CTGGGCAACC TGGCCCAGTC 
GGGCCTGCTG GTGTTCGTGG TTCTCAGGGT AGCCAAGGTC CCGCTGGTCC CCCTGGTCCC 
CCTGGACCTC CTGGTCCTCC TGGTGTCAGC GGAGGTGGCT ATGACTTTGG TTTTGAAGGA 
GACTTCTACA GGGCTGACCA GCCTCGCTCC CAGCCTTCAC TCAGACCCAA GGACTATGAA 
GTTGATGCAA CTCTGAAATC TCTCAATAAC CAGATTGAGA CCCTTCTCAC TCCTGAAGGC 
TCTAGAAAGA ACCCTGCTCG CACGTGCCGG GACTTAAGAC TCAGCCACCC AGAGTGGAAC 
AGCGATTACT ACTGGATTGA CCCTAACCAA GGATGTACTA TGGATGCCAT CAAAGTGTAC 
TGTGATTTCT CTACTGGTGA AACCTGCATC CAGGCCCAAC CTGTAAACAC CCCAGCGAAG 
AACTCATACA GCCGCGCCCA GGCCAACAAG CATGTCTGGT TAGGAGAGAC TATCAATGGT 
GGCAGCCAGT TTGAATACAA CGTAGAAGGG GTGTCCTCCA AGGAAATGGC AACTCAGCTC 
GCCTTCATGC GCCTGCTAGC CAACCGTGCT TCTCAGAACA TCACCTACCA CTGCAAGAAC 
AGCATTGCGT ACCTGGATGA GGAGACGGGC AGCTTGAACA AGGCTGTGCT TCTGCAGGGT 
TCCAACGATG TTGAACTTGT TGCTGAGGGC AACAGCAGGT TCACCTACTC TGTCCTAGTC 
GATGGCTGCT CCAAAAAGAC AAATGAATGG GGCAAGACAA TCATTGAATA CAAAACAAAT 
AAGCCATCTC GCCTGCCGTT CCTTGACATT GCACCTCTGG ACATTGGTGG TGCTGACCAA 
GAATTCCGTG TGGAGGTTGG CCCCGTCTGT TTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031769.2
CDS	223..4341
Translation	MLSFVDTRTLLLLAVTSCLATCQYLQSGSVRKGPTGDRGPRGQRGPAGPRGRDGVDGPMGPPGPPGSPGPPGSPAPPGLTGNFAAQYSDKGVSSGPGPMGLMGPRGPPGAVGAPGPQGFQGPAGEPGEPGQTGPAGPRGPAGSPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGVKGHSGMDGLKGQPGAQGVKGEPGAPGENGTPGQAGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGPVGNPGPAGPAGPRGEVGLPGLSGPVGPPGNPGTNGLTGAKGATGLPGVAGAPGLPGPRGIPGPAGAAGATGARGLVGEPGPAGSKGESGNKGEPGSVGAQGPPGPSGEEGKRGSPGEAGSAGPAGPPGLRGSPGSRGLPGADGRAGVMGPPGNRGSTGPAGIRGPNGDAGRPGEPGLMGPRGLPGSPGNVGPSGKEGPVGLPGIDGRPGPIGPAGPRGEAGNIGFPGPKGPSGDPGKPGERGHPGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPAGPPGFQGLPGPSGTTGEVGKPGERGLPGEFGLPGPAGPRGERGTPGESGAAGPSGPIGSRGPSGAPGPDGNKGEAGAVGAPGSAGASGPGGLPGERGAAGIPGGKGEKGETGLRGDTGNTGRDGARGIPGAVGAPGPAGASGDRGEAGAAGPSGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGEKGTKGPKGENGIVGPTGSVGAAGPSGPNGPPGPVGSRGDGGPPGMTGFPGAAGRTGPPGPSGIAGPPGPPGAAGKEGIRGPRGDQGPVGRTGETGASGPPGFVGEKGPSGEPGTAGAPGTAGPQGLLGAPGILGLPGSRGERGLPGIAGALGEPGPLGISGPPGARGPPGAVGSPGVNGAPGEAGRDGNPGSDGPPGRDGQPGHKGERGYPGSIGPTGAAGAPGPHGSVGPAGKHGNRGEPGPAGSVGPVGAVGPRGPSGPQGIRGDKGEPGDKGHRGLPGLKGYSGLQGLPGLAGLHGDQGAPGPVGPAGPRGPAGPSGPVGKDGRSGQPGPVGPAGVRGSQGSQGPAGPPGPPGPPGPPGVSGGGYDFGFEGDFYRADQPRSQPSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWNSDYYWIDPNQGCTMDAIKVYCDFSTGETCIQAQPVNTPAKNSYSRAQANKHVWLGETINGGSQFEYNVEGVSSKEMATQLAFMRLLANRASQNITYHCKNSIAYLDEETGSLNKAVLLQGSNDVELVAEGNSRFTYSVLVDGCSKKTNEWGKTIIEYKTNKPSRLPFLDIAPLDIGGADQEFRVEVGPVCFK

Target ORF information:

RefSeq Version	NM_007743.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type I, alpha 2 (Col1a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007743.3

ORF Insert Sequence:

ATGCTCAGCT TTGTGGATAC GCGGACTCTG TTGCTGCTTG CAGTAACTTC GTGCCTAGCA 
ACATGCCAAT ATTTGCAATC GGGATCAGTA CGAAAGGGCC CCACTGGAGA CAGAGGACCA 
CGTGGACAAA GGGGTCCCGC AGGTCCCCGA GGCAGAGATG GTGTTGATGG TCCCATGGGC 
CCTCCTGGTC CCCCTGGCTC CCCTGGTCCT CCCGGCTCCC CTGCCCCCCC TGGTCTTACT 
GGGAACTTTG CTGCTCAGTA TTCTGACAAA GGAGTTTCAT CTGGCCCTGG ACCAATGGGT 
TTAATGGGAC CCAGAGGCCC TCCTGGTGCT GTTGGAGCCC CTGGCCCTCA AGGTTTCCAA 
GGACCTGCTG GTGAACCTGG TGAGCCTGGT CAAACGGGTC CTGCAGGTCC CCGTGGTCCA 
GCTGGCTCTC CTGGCAAGGC TGGTGAGGAT GGTCACCCTG GAAAACCCGG AAGACCTGGG 
GAGAGAGGAG TCGTTGGACC ACAGGGTGCT CGTGGTTTCC CCGGGACTCC TGGACTTCCT 
GGCTTCAAAG GCGTGAAAGG ACACAGTGGT ATGGATGGAT TAAAGGGACA GCCTGGTGCA 
CAGGGTGTGA AGGGTGAACC CGGTGCCCCT GGAGAGAATG GAACTCCAGG TCAAGCAGGA 
GCCCGAGGGC TTCCTGGTGA GAGAGGGCGT GTCGGAGCTC CTGGTCCAGC TGGTGCCCGA 
GGCAGTGATG GAAGCGTTGG CCCCGTCGGC CCTGCTGGTC CCATTGGGTC TGCTGGACCC 
CCTGGTTTCC CAGGTGCTCC TGGTCCCAAG GGTGAACTTG GGCCCGTTGG TAACCCTGGC 
CCTGCTGGTC CTGCTGGTCC CCGTGGTGAA GTGGGTCTTC CAGGTCTTTC TGGCCCTGTT 
GGACCTCCTG GCAACCCTGG AACAAATGGG CTCACTGGCG CCAAGGGTGC TACTGGACTC 
CCTGGTGTCG CTGGGGCTCC CGGTCTCCCT GGCCCCCGTG GTATTCCTGG CCCCGCTGGT 
GCTGCTGGTG CTACAGGTGC CAGAGGACTT GTTGGTGAGC CTGGTCCTGC TGGCTCCAAA 
GGAGAATCCG GTAACAAGGG TGAGCCTGGC TCTGTTGGAG CCCAAGGTCC TCCTGGTCCC 
AGCGGTGAAG AAGGAAAGAG AGGGTCTCCC GGAGAAGCTG GATCTGCTGG CCCTGCAGGG 
CCTCCAGGGC TTAGAGGCAG CCCTGGTTCT CGAGGTCTTC CTGGAGCTGA TGGCAGAGCT 
GGTGTAATGG GTCCTCCTGG CAATCGTGGT TCAACCGGCC CTGCTGGAAT CCGAGGTCCT 
AATGGAGATG CCGGTCGACC TGGGGAACCT GGTCTCATGG GACCCAGAGG TCTTCCTGGG 
TCTCCTGGAA ATGTTGGCCC ATCTGGTAAA GAAGGCCCTG TGGGTCTCCC TGGCATTGAT 
GGCAGACCTG GCCCAATCGG CCCAGCTGGA CCAAGAGGTG AAGCTGGCAA CATCGGATTC 
CCTGGACCCA AAGGCCCCTC TGGTGATCCT GGCAAACCTG GTGAGAGAGG ACACCCCGGT 
CTTGCTGGTG CTCGGGGAGC TCCAGGACCC GATGGCAACA ATGGAGCTCA GGGCCCCCCT 
GGACCCCAGG GTGTTCAAGG TGGCAAAGGT GAACAAGGCC CTGCTGGTCC TCCTGGCTTC 
CAGGGTCTCC CTGGTCCCTC AGGTACTACT GGAGAAGTTG GCAAGCCCGG AGAAAGGGGT 
CTTCCTGGTG AATTCGGTCT CCCTGGTCCT GCTGGTCCAA GAGGAGAACG TGGTACCCCG 
GGTGAGAGTG GAGCTGCTGG CCCTTCTGGT CCTATTGGAA GCCGAGGTCC CAGTGGAGCC 
CCAGGGCCTG ATGGAAACAA GGGTGAAGCT GGTGCAGTCG GTGCTCCAGG CAGTGCTGGT 
GCCTCTGGGC CTGGTGGGCT TCCAGGAGAG AGGGGTGCTG CTGGCATACC TGGGGGCAAA 
GGAGAAAAGG GTGAAACTGG TCTCCGAGGT GACACTGGCA ACACTGGTAG AGATGGTGCT 
CGTGGCATTC CTGGTGCTGT AGGTGCCCCT GGTCCTGCTG GGGCCTCAGG TGACCGGGGT 
GAAGCTGGTG CTGCCGGTCC TTCTGGCCCA GCTGGTCCTC GGGGTAGCCC TGGTGAACGT 
GGTGAAGTTG GCCCTGCTGG CCCCAATGGA TTTGCTGGTC CTGCTGGTGC TGCTGGCCAA 
CCCGGTGCTA AAGGAGAGAA GGGAACCAAA GGGCCTAAGG GTGAAAATGG CATTGTTGGT 
CCAACCGGGT CTGTTGGAGC TGCTGGCCCA TCTGGTCCTA ATGGCCCACC TGGTCCTGTT 
GGAAGTCGTG GTGATGGAGG GCCCCCTGGT ATGACTGGCT TCCCTGGTGC TGCTGGACGG 
ACTGGTCCCC CCGGACCCAG TGGTATTGCT GGCCCCCCTG GCCCCCCTGG TGCTGCTGGA 
AAGGAAGGGA TTCGTGGTCC CCGTGGCGAC CAAGGTCCAG TTGGCCGAAC TGGAGAAACA 
GGTGCATCTG GACCCCCTGG ATTTGTTGGT GAAAAGGGTC CCTCTGGAGA ACCTGGTACC 
GCAGGAGCCC CTGGTACCGC AGGTCCTCAG GGTCTTCTTG GTGCTCCCGG TATTCTGGGT 
CTCCCTGGCT CTAGAGGTGA ACGTGGTCTG CCAGGTATTG CTGGTGCTCT GGGTGAACCT 
GGTCCTCTGG GCATCTCAGG CCCTCCTGGT GCCCGTGGTC CCCCTGGTGC TGTGGGCAGC 
CCTGGAGTTA ATGGTGCCCC TGGTGAAGCT GGTCGTGATG GTAACCCTGG CAGCGATGGT 
CCCCCAGGTC GTGATGGTCA GCCTGGACAC AAGGGAGAGC GTGGTTACCC TGGCAGCATT 
GGTCCGACTG GTGCTGCAGG TGCCCCTGGT CCTCACGGTT CTGTGGGTCC TGCTGGGAAA 
CATGGAAACC GAGGTGAACC TGGTCCTGCC GGTTCTGTTG GTCCTGTTGG TGCTGTTGGT 
CCAAGAGGTC CTAGTGGTCC TCAAGGCATC CGAGGTGACA AAGGAGAACC TGGTGATAAA 
GGGCACAGGG GTCTTCCTGG CTTAAAAGGA TACAGTGGAT TGCAGGGTCT TCCTGGTCTT 
GCTGGCCTAC ATGGTGACCA AGGAGCTCCT GGTCCTGTGG GTCCTGCTGG TCCCAGGGGT 
CCTGCTGGGC CTTCTGGACC CGTTGGCAAA GATGGTAGAT CTGGGCAACC TGGCCCAGTC 
GGGCCTGCTG GTGTTCGTGG TTCTCAGGGT AGCCAAGGTC CCGCTGGTCC CCCTGGTCCC 
CCTGGACCTC CTGGTCCTCC TGGTGTCAGC GGAGGTGGCT ATGACTTTGG TTTTGAAGGA 
GACTTCTACA GGGCTGACCA GCCTCGCTCC CAGCCTTCAC TCAGACCCAA GGACTATGAA 
GTTGATGCAA CTCTGAAATC TCTCAATAAC CAGATTGAGA CCCTTCTCAC TCCTGAAGGC 
TCTAGAAAGA ACCCTGCTCG CACGTGCCGG GACTTAAGAC TCAGCCACCC AGAGTGGAAC 
AGCGATTACT ACTGGATTGA CCCTAACCAA GGATGTACTA TGGATGCCAT CAAAGTGTAC 
TGTGATTTCT CTACTGGTGA AACCTGCATC CAGGCCCAAC CTGTAAACAC CCCAGCGAAG 
AACTCATACA GCCGCGCCCA GGCCAACAAG CATGTCTGGT TAGGAGAGAC TATCAATGGT 
GGCAGCCAGT TTGAATACAA CGTAGAAGGG GTGTCCTCCA AGGAAATGGC AACTCAGCTC 
GCCTTCATGC GCCTGCTAGC CAACCGTGCT TCTCAGAACA TCACCTACCA CTGCAAGAAC 
AGCATTGCGT ACCTGGATGA GGAGACGGGC AGCTTGAACA AGGCTGTGCT TCTGCAGGGT 
TCCAACGATG TTGAACTTGT TGCTGAGGGC AACAGCAGGT TCACCTACTC TGTCCTAGTC 
GATGGCTGCT CCAAAAAGAC AAATGAATGG GGCAAGACAA TCATTGAATA CAAAACAAAT 
AAGCCATCTC GCCTGCCGTT CCTTGACATT GCACCTCTGG ACATTGGTGG TGCTGACCAA 
GAATTCCGTG TGGAGGTTGG CCCCGTCTGT TTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of flow responsive Tie1 results in Impaired Aortic valve remodeling.
Developmental biology455(1)73-84(2019 Nov)
Qu X,Violette K,Sewell-Loftin MK,Soslow J,Saint-Jean L,Hinton RB,Merryman WD,Baldwin HS

Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia.
Human molecular genetics28(9)1429-1444(2019 05)
Chao CM,Moiseenko A,Kosanovic D,Rivetti S,El Agha E,Wilhelm J,Kampschulte M,Yahya F,Ehrhardt H,Zimmer KP,Barreto G,Rizvanov AA,Schermuly RT,Reiss I,Morty RE,Rottier RJ,Bellusci S,Zhang JS

Transcriptome analysis of mouse and human sinoatrial node cells reveals a conserved genetic program.
Development (Cambridge, England)146(8)(2019 04)
van Eif VWW,Stefanovic S,van Duijvenboden K,Bakker M,Wakker V,de Gier-de Vries C,Zaffran S,Verkerk AO,Boukens BJ,Christoffels VM

Endoplasmic reticulum stress is induced in growth plate hypertrophic chondrocytes in G610C mouse model of osteogenesis imperfecta.
Biochemical and biophysical research communications509(1)235-240(2019 01)
Scheiber AL,Guess AJ,Kaito T,Abzug JM,Enomoto-Iwamoto M,Leikin S,Iwamoto M,Otsuru S

TBX2 and TBX3 act downstream of canonical WNT signaling in patterning and differentiation of the mouse ureteric mesenchyme.
Development (Cambridge, England)145(23)(2018 11)
Aydoğdu N,Rudat C,Trowe MO,Kaiser M,Lüdtke TH,Taketo MM,Christoffels VM,Moon A,Kispert A

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Col1a2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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