Clcn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcn2
Entrez Gene ID	12724
Full Name	chloride channel, voltage-sensitive 2
Synonyms	AL118368,ClC-2,Clc2,nmf240
General protein information	Preferred Names chloride channel, voltage-sensitive 2 Names chloride channel protein 2 chloride channel 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 B1\|16 12.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316862.2	XP_017172351.1	chloride channel protein 2 isoform X1
XM_006521749.3	XP_006521812.1	chloride channel protein 2 isoform X2
XM_030248952.1	XP_030104812.1	chloride channel protein 2 isoform X2
NM_009900.2	NP_034030.2	chloride channel protein 2
XM_006521749.4	XP_006521812.1	chloride channel protein 2 isoform X2
XM_017316862.1	XP_017172351.1	chloride channel protein 2 isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	clh-4	NP_001024885.1
Pan troglodytes (chimpanzee)	CLCN2	XP_003950294.1
Caenorhabditis elegans (roundworm)	clh-3	NP_001022060.1
Rattus norvegicus (Norway rat)	LOC100912455	XP_003751129.1
Canis lupus familiaris (dog)	CLCN2	XP_005639832.1
Bos taurus (cattle)	CLCN2	NP_001095957.1
Rattus norvegicus (Norway rat)	Clcn2	NP_058833.1
Xenopus tropicalis (tropical clawed frog)	clcn2	XP_004914466.1
Macaca mulatta (Rhesus monkey)	CLCN2	XP_002808378.1
Mus musculus (house mouse)	Clcn2	NP_034030.2
Drosophila melanogaster (fruit fly)	ClC-a	NP_001097752.1
Danio rerio (zebrafish)	LOC100333644	XP_002667721.3
Homo sapiens (human)	CLCN2	NP_004357.3
Gallus gallus (chicken)	CLCN2	XP_423073.4
Anopheles gambiae (African malaria mosquito)	GPRMGL4	XP_003436287.1

Gene Ontology
Bibliography

Related articles in PubMed

Pathogenesis of hypertension in a mouse model for human CLCN2 related hyperaldosteronism.
Göppner C, Orozco IJ, Hoegg-Beiler MB, Soria AH, Hübner CA, Fernandes-Rosa FL, Boulkroun S, Zennaro MC, Jentsch TJ
Nature communications10(1)4678(2019 10)

Knockout of ClC-2 reveals critical functions of adherens junctions in colonic homeostasis and tumorigenicity.
Jin Y, Ibrahim D, Magness ST, Blikslager AT
American journal of physiology. Gastrointestinal and liver physiology315(6)G966-G979(2018 12)

A gain-of-function mutation in the CLCN2 chloride channel gene causes primary aldosteronism.
Fernandes-Rosa FL, Daniil G, Orozco IJ, Göppner C, El Zein R, Jain V, Boulkroun S, Jeunemaitre X, Amar L, Lefebvre H, Schwarzmayr T, Strom TM, Jentsch TJ, Zennaro MC
Nature genetics50(3)355-361(2018 03)

Lack of ClC-2 Alleviates High Fat Diet-Induced Insulin Resistance and Non-Alcoholic Fatty Liver Disease.
Fu D, Cui H, Zhang Y
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology45(6)2187-2198(2018)

Mouse models of human ocular disease for translational research.
Krebs MP, Collin GB, Hicks WL, Yu M, Charette JR, Shi LY, Wang J, Naggert JK, Peachey NS, Nishina PM
PloS one12(8)e0183837(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Clcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21278	NM_009900.2 Latest version!	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu51839	XM_030248952.1 Latest version!	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu51839	XM_006521749.4 Latest version!	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu79256	XM_017316862.2 Latest version!	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu51839	XM_006521749.3	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu79256	XM_017316862.1	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcn2 ( NM_009900.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034030.2 Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), mRNA.

Price & Availability↑

CloneID	OMu21278
Clone ID Related Accession (Same CDS sequence)	NM_009900.2
Accession Version	NM_009900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Mus musculus(house mouse)
Product	chloride channel protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT010490.10, AF097415.1, BC120699.1 and CK633852.1. On Jan 15, 2008 this sequence version replaced NM_009900.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF097415.1, AK155104.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTG CAACGGCTGC GGCGGCCGCG GCGGCGGCGG CGGGGGAAGG GATGGAGCCT 
CGAGCGCTGC AGTACGAGCA GACCCTGATG TATGGCCGGT ACACTCAGGA ACTCGGGGCC 
TTTGCCAAAG AGGAAGCTGC TCGTATTCGC CTGGGAGGGC CTGAGCCCTG GAAGGGGTCC 
CCTTCTGCCC GGGCTACCCC AGAGCTCCTA GAATATGGAC AGAGCCGATG TGCCAGATGT 
CGCATTTGTT CTGTACGCTG CCACAAGTTC CTGGTGTCCA GGGTCGGTGA AGACTGGATC 
TTCCTGGTTC TGTTGGGGCT CCTCATGGCA CTGGTCAGCT GGGCTATGGA CTATGCCATC 
GCTGTCTGTC TACAGGCTCA GCAATGGATG TCCCGGGGCT TAAACACCAA CATCTTACTC 
CAGTACCTGG CTTGGGTTAC CTACCCCGTG GTCCTCATCA CTTTCTCTGC TGGATTCACC 
CAGATCCTGG CCCCACAGGC TGTCGGGTCT GGCATCCCCG AAATGAAAAC CATCCTTCGG 
GGAGTGGTGC TGAAAGAATA CCTCACCCTC AAGACCTTTG TAGCTAAGGT CATTGGGCTA 
ACCTGTGCCC TGGGCAGTGG GATGCCCCTT GGCAAAGAGG GACCCTTTGT GCACATTGCC 
AGCATGTGTG CCGCCCTTCT CAGCAAGTTC CTCTCCCTCT TTGGGGGTAT CTATGAGCAT 
GAGTCCCGGA ACACGGAGAT GCTAGCTGCT GCATGCGCAG TAGGAGTGGG CTGCTGCTTT 
GCCGCACCAA TCGGAGGGGT CCTATTCAGC ATTGAAGTCA CCTCCACCTT CTTCGCTGTT 
AGGAACTACT GGCGGGGCTT CTTTGCGGCC ACCTTCAGTG CCTTCATCTT TCGGGTCTTG 
GCAGTGTGGA ACCGTGATGA AGAAACCATC ACAGCTCTCT TCAAAACTCG GTTCCGACTC 
GACTTCCCAT TTGACCTGCA AGAGCTGCCA GCCTTTGCTG TCATTGGCAT TGCTAGTGGC 
TTCGGGGGAG CCCTCTTTGT CTACCTGAAC CGGAAGATTG TCCAGGTGAT GCGGAAGCAA 
AAAACCATCA ACCGCTTCCT CATGAGGAAA CGGCTGCTCT TCCCGGCACT GGTGACTCTG 
CTCATCTCCA CTCTGACCTT CCCCCCTGGC TTTGGACAGT TCATGGCCGG ACAGCTCTCA 
CAGAAGGAGA CCCTAGTCAC TCTGTTTGAC AACCGGACGT GGGTCCGCCA GGGCCTGGTT 
GAGGATCTAG AGCTACCCAG CACTTCACAG GCCTGGAGCC CACCACGTGC CAATGTCTTC 
CTTACTCTGG TCATCTTCAT CCTCATGAAG TTCTGGATGT CTGCACTGGC TACCACTATC 
CCAGTGCCCT GTGGGGCCTT CATGCCTGTC TTTGTCATTG GAGCGGCATT TGGGCGGCTG 
GTGGGCGAAA GCATGGCCGC CTGGTTCCCA GATGGGATTC ACACAGATAG CAGCACCTAC 
CGAATTGTAC CTGGAGGCTA TGCTGTGGTC GGGGCGGCTG CACTCGCAGG AGCAGTGACA 
CACACAGTGT CCACAGCAGT GATTGTCTTC GAGCTCACGG GCCAGATCGC TCACATTCTG 
CCTGTCATGA TTGCTGTCAT CCTGGCTAAT GCTGTTGCCC AGAGCCTGCA GCCATCGCTC 
TATGACAGTA TCATCCGCAT CAAGAAGCTG CCCTACCTAC CTGAGCTGGG CTGGGGCCGC 
CACCAGCAGT ACCGGGTGCG AGTCGAGGAC ATCATGGTTC GGGATGTACC CCATGTAGCC 
CTCAGCTGCA CTTTTCGGGA CCTGCGGTTG GCACTGCACA GAACCAAGGG CCGTATGTTG 
GCCCTAGTGG AGTCTCCTGA GTCCATGATC CTACTGGGAT CCATCGAACG CTCACAGGTG 
GTAGCACTAC TAGGAGCCCA GCTGAGCCCA GCGCGCAGGC GGCAGCACAT GCAAAAGCTA 
AGAAAAGCCC AGCTGTCTCC ACCGTCGGAT CAGGAGAGCC CCCCTAGCTC CGAGACATCT 
ATCCGCTTCC AGGTGAACAC AGAGGACTCG GGCTTCTCTG GAGCCCACGG GCAGACTCAC 
AAGCCCCTGA AGCCTGCTCT AAAGAGAGGG CCCAGCAACA GTACAAGCCT GCAGGAAGGT 
ACCACAGGCA ACATGGAGTC AGCAGGCATT GCCCTCAGAA GCCTCTTCTG TGGCAGTCCA 
CCTCTGGAGG CAACATCAGA ATTGGAAAAG TCAGAATCCT GTGACAAGCG CAAGCTGAAG 
CGGGTCCGAA TCTCCCTGGC GAGTGACTCA GACCCGGAAG CCGAGATGAG TCCTGAGGAG 
ATCTTAGAGT GGGAAGAACA GCAGCTAGAT GAGCCAGTCA ACTTCAGTGA CTGCAAAATC 
GACCCTGCCC CCTTCCAGCT GGTGGAGCGG ACTTCTTTGC ACAAGACCCA CACCATCTTC 
TCATTGCTGG GAGTGGACCA TGCTTATGTC ACCAGCATTG GCAGACTCAT TGGGATTGTC 
ACCCTAAAGG AGCTCCGGAA GGCCATTGAA GGCTCTGTCA CAGCACAGGG TGTTAAAGTC 
AGGCCACCCC TCGCCAGTTT CCGGGACAGT GCCACCAGCA GCAGTGACAC AGAGACCACT 
GAGGTGCATG CGCTCTGGGG GCCAAGATCC CGCCACGGCC TCCCACGAGA GGGTACCCCC 
TCCGACAGTG ATGACAAGTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034030.2
CDS	642..3368
Translation	MAAATAAAAAAAAAGEGMEPRALQYEQTLMYGRYTQELGAFAKEEAARIRLGGPEPWKGSPSARATPELLEYGQSRCARCRICSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWAMDYAIAVCLQAQQWMSRGLNTNILLQYLAWVTYPVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFVAKVIGLTCALGSGMPLGKEGPFVHIASMCAALLSKFLSLFGGIYEHESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLELPSTSQAWSPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQLSPPSDQESPPSSETSIRFQVNTEDSGFSGAHGQTHKPLKPALKRGPSNSTSLQEGTTGNMESAGIALRSLFCGSPPLEATSELEKSESCDKRKLKRVRISLASDSDPEAEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ

Target ORF information:

RefSeq Version	NM_009900.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009900.2

ORF Insert Sequence:

ATGGCGGCTG CAACGGCTGC GGCGGCCGCG GCGGCGGCGG CGGGGGAAGG GATGGAGCCT 
CGAGCGCTGC AGTACGAGCA GACCCTGATG TATGGCCGGT ACACTCAGGA ACTCGGGGCC 
TTTGCCAAAG AGGAAGCTGC TCGTATTCGC CTGGGAGGGC CTGAGCCCTG GAAGGGGTCC 
CCTTCTGCCC GGGCTACCCC AGAGCTCCTA GAATATGGAC AGAGCCGATG TGCCAGATGT 
CGCATTTGTT CTGTACGCTG CCACAAGTTC CTGGTGTCCA GGGTCGGTGA AGACTGGATC 
TTCCTGGTTC TGTTGGGGCT CCTCATGGCA CTGGTCAGCT GGGCTATGGA CTATGCCATC 
GCTGTCTGTC TACAGGCTCA GCAATGGATG TCCCGGGGCT TAAACACCAA CATCTTACTC 
CAGTACCTGG CTTGGGTTAC CTACCCCGTG GTCCTCATCA CTTTCTCTGC TGGATTCACC 
CAGATCCTGG CCCCACAGGC TGTCGGGTCT GGCATCCCCG AAATGAAAAC CATCCTTCGG 
GGAGTGGTGC TGAAAGAATA CCTCACCCTC AAGACCTTTG TAGCTAAGGT CATTGGGCTA 
ACCTGTGCCC TGGGCAGTGG GATGCCCCTT GGCAAAGAGG GACCCTTTGT GCACATTGCC 
AGCATGTGTG CCGCCCTTCT CAGCAAGTTC CTCTCCCTCT TTGGGGGTAT CTATGAGCAT 
GAGTCCCGGA ACACGGAGAT GCTAGCTGCT GCATGCGCAG TAGGAGTGGG CTGCTGCTTT 
GCCGCACCAA TCGGAGGGGT CCTATTCAGC ATTGAAGTCA CCTCCACCTT CTTCGCTGTT 
AGGAACTACT GGCGGGGCTT CTTTGCGGCC ACCTTCAGTG CCTTCATCTT TCGGGTCTTG 
GCAGTGTGGA ACCGTGATGA AGAAACCATC ACAGCTCTCT TCAAAACTCG GTTCCGACTC 
GACTTCCCAT TTGACCTGCA AGAGCTGCCA GCCTTTGCTG TCATTGGCAT TGCTAGTGGC 
TTCGGGGGAG CCCTCTTTGT CTACCTGAAC CGGAAGATTG TCCAGGTGAT GCGGAAGCAA 
AAAACCATCA ACCGCTTCCT CATGAGGAAA CGGCTGCTCT TCCCGGCACT GGTGACTCTG 
CTCATCTCCA CTCTGACCTT CCCCCCTGGC TTTGGACAGT TCATGGCCGG ACAGCTCTCA 
CAGAAGGAGA CCCTAGTCAC TCTGTTTGAC AACCGGACGT GGGTCCGCCA GGGCCTGGTT 
GAGGATCTAG AGCTACCCAG CACTTCACAG GCCTGGAGCC CACCACGTGC CAATGTCTTC 
CTTACTCTGG TCATCTTCAT CCTCATGAAG TTCTGGATGT CTGCACTGGC TACCACTATC 
CCAGTGCCCT GTGGGGCCTT CATGCCTGTC TTTGTCATTG GAGCGGCATT TGGGCGGCTG 
GTGGGCGAAA GCATGGCCGC CTGGTTCCCA GATGGGATTC ACACAGATAG CAGCACCTAC 
CGAATTGTAC CTGGAGGCTA TGCTGTGGTC GGGGCGGCTG CACTCGCAGG AGCAGTGACA 
CACACAGTGT CCACAGCAGT GATTGTCTTC GAGCTCACGG GCCAGATCGC TCACATTCTG 
CCTGTCATGA TTGCTGTCAT CCTGGCTAAT GCTGTTGCCC AGAGCCTGCA GCCATCGCTC 
TATGACAGTA TCATCCGCAT CAAGAAGCTG CCCTACCTAC CTGAGCTGGG CTGGGGCCGC 
CACCAGCAGT ACCGGGTGCG AGTCGAGGAC ATCATGGTTC GGGATGTACC CCATGTAGCC 
CTCAGCTGCA CTTTTCGGGA CCTGCGGTTG GCACTGCACA GAACCAAGGG CCGTATGTTG 
GCCCTAGTGG AGTCTCCTGA GTCCATGATC CTACTGGGAT CCATCGAACG CTCACAGGTG 
GTAGCACTAC TAGGAGCCCA GCTGAGCCCA GCGCGCAGGC GGCAGCACAT GCAAAAGCTA 
AGAAAAGCCC AGCTGTCTCC ACCGTCGGAT CAGGAGAGCC CCCCTAGCTC CGAGACATCT 
ATCCGCTTCC AGGTGAACAC AGAGGACTCG GGCTTCTCTG GAGCCCACGG GCAGACTCAC 
AAGCCCCTGA AGCCTGCTCT AAAGAGAGGG CCCAGCAACA GTACAAGCCT GCAGGAAGGT 
ACCACAGGCA ACATGGAGTC AGCAGGCATT GCCCTCAGAA GCCTCTTCTG TGGCAGTCCA 
CCTCTGGAGG CAACATCAGA ATTGGAAAAG TCAGAATCCT GTGACAAGCG CAAGCTGAAG 
CGGGTCCGAA TCTCCCTGGC GAGTGACTCA GACCCGGAAG CCGAGATGAG TCCTGAGGAG 
ATCTTAGAGT GGGAAGAACA GCAGCTAGAT GAGCCAGTCA ACTTCAGTGA CTGCAAAATC 
GACCCTGCCC CCTTCCAGCT GGTGGAGCGG ACTTCTTTGC ACAAGACCCA CACCATCTTC 
TCATTGCTGG GAGTGGACCA TGCTTATGTC ACCAGCATTG GCAGACTCAT TGGGATTGTC 
ACCCTAAAGG AGCTCCGGAA GGCCATTGAA GGCTCTGTCA CAGCACAGGG TGTTAAAGTC 
AGGCCACCCC TCGCCAGTTT CCGGGACAGT GCCACCAGCA GCAGTGACAC AGAGACCACT 
GAGGTGCATG CGCTCTGGGG GCCAAGATCC CGCCACGGCC TCCCACGAGA GGGTACCCCC 
TCCGACAGTG ATGACAAGTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn2 ( XM_006521749.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521812.1 Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu51839
Clone ID Related Accession (Same CDS sequence)	XM_006521749.3 , XM_030248952.1 , XM_006521749.4
Accession Version	XM_006521749.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	chloride channel protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA GGAGTGGGCT GCTGCTTTGC CGCACCAATC GGAGGAACTA CTGGCGGGGC 
TTCTTTGCGG CCACCTTCAG TGCCTTCATC TTTCGGGTCT TGGCAGTGTG GAACCGTGAT 
GAAGAAACCA TCACAGCTCT CTTCAAAACT CGGTTCCGAC TCGACTTCCC ATTTGACCTG 
CAAGAGCTGC CAGCCTTTGC TGTCATTGGC ATTGCTAGTG GCTTCGGGGG AGCCCTCTTT 
GTCTACCTGA ACCGGAAGAT TGTCCAGGTG ATGCGGAAGC AAAAAACCAT CAACCGCTTC 
CTCATGAGGA AACGGCTGCT CTTCCCGGCA CTGGTGACTC TGCTCATCTC CACTCTGACC 
TTCCCCCCTG GCTTTGGACA GTTCATGGCC GGACAGCTCT CACAGAAGGA GACCCTAGTC 
ACTCTGTTTG ACAACCGGAC GTGGGTCCGC CAGGGCCTGG TTGAGGATCT AGAGCTACCC 
AGCACTTCAC AGGCCTGGAG CCCACCACGT GCCAATGTCT TCCTTACTCT GGTCATCTTC 
ATCCTCATGA AGTTCTGGAT GTCTGCACTG GCTACCACTA TCCCAGTGCC CTGTGGGGCC 
TTCATGCCTG TCTTTGTCAT TGGAGCGGCA TTTGGGCGGC TGGTGGGCGA AAGCATGGCC 
GCCTGGTTCC CAGATGGGAT TCACACAGAT AGCAGCACCT ACCGAATTGT ACCTGGAGGC 
TATGCTGTGG TCGGGGCGGC TGCACTCGCA GGAGCAGTGA CACACACAGT GTCCACAGCA 
GTGATTGTCT TCGAGCTCAC GGGCCAGATC GCTCACATTC TGCCTGTCAT GATTGCTGTC 
ATCCTGGCTA ATGCTGTTGC CCAGAGCCTG CAGCCATCGC TCTATGACAG TATCATCCGC 
ATCAAGAAGC TGCCCTACCT ACCTGAGCTG GGCTGGGGCC GCCACCAGCA GTACCGGGTG 
CGAGTCGAGG ACATCATGGT TCGGGATGTA CCCCATGTAG CCCTCAGCTG CACTTTTCGG 
GACCTGCGGT TGGCACTGCA CAGAACCAAG GGCCGTATGT TGGCCCTAGT GGAGTCTCCT 
GAGTCCATGA TCCTACTGGG ATCCATCGAA CGCTCACAGG TGGTAGCACT ACTAGGAGCC 
CAGCTGAGCC CAGCGCGCAG GCGGCAGCAC ATGCAAAAGC TAAGAAAAGC CCAGCTGTCT 
CCACCGTCGG ATCAGGAGAG CCCCCCTAGC TCCGAGACAT CTATCCGCTT CCAGGTGAAC 
ACAGAGGACT CGGGCTTCTC TGGAGCCCAC GGGCAGACTC ACAAGCCCCT GAAGCCTGCT 
CTAAAGAGAG GGCCCAGCAA CAGTACAAGC CTGCAGGAAG GTACCACAGG CAACATGGAG 
TCAGCAGGCA TTGCCCTCAG AAGCCTCTTC TGTGGCAGTC CACCTCTGGA GGCAACATCA 
GAATTGGAAA AGTCAGAATC CTGTGACAAG CGCAAGCTGA AGCGGGTCCG AATCTCCCTG 
GCGAGTGACT CAGACCCGGA AGCCGAGATG AGTCCTGAGG AGATCTTAGA GTGGGAAGAA 
CAGCAGCTAG ATGAGCCAGT CAACTTCAGT GACTGCAAAA TCGACCCTGC CCCCTTCCAG 
CTGGTGGAGC GGACTTCTTT GCACAAGACC CACACCATCT TCTCATTGCT GGGAGTGGAC 
CATGCTTATG TCACCAGCAT TGGCAGACTC ATTGGGATTG TCACCCTAAA GGAGCTCCGG 
AAGGCCATTG AAGGCTCTGT CACAGCACAG GGTGTTAAAG TCAGGCCACC CCTCGCCAGT 
TTCCGGGACA GTGCCACCAG CAGCAGTGAC ACAGAGACCA CTGAGGTGCA TGCGCTCTGG 
GGGCCAAGAT CCCGCCACGG CCTCCCACGA GAGGGTACCC CCTCCGACAG TGATGACAAG 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521812.1
CDS	114..2042
Translation	MRSRSGLLLCRTNRRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLELPSTSQAWSPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQLSPPSDQESPPSSETSIRFQVNTEDSGFSGAHGQTHKPLKPALKRGPSNSTSLQEGTTGNMESAGIALRSLFCGSPPLEATSELEKSESCDKRKLKRVRISLASDSDPEAEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ

Target ORF information:

RefSeq Version	XM_006521749.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521749.3

ORF Insert Sequence:

ATGCGCAGTA GGAGTGGGCT GCTGCTTTGC CGCACCAATC GGAGGAACTA CTGGCGGGGC 
TTCTTTGCGG CCACCTTCAG TGCCTTCATC TTTCGGGTCT TGGCAGTGTG GAACCGTGAT 
GAAGAAACCA TCACAGCTCT CTTCAAAACT CGGTTCCGAC TCGACTTCCC ATTTGACCTG 
CAAGAGCTGC CAGCCTTTGC TGTCATTGGC ATTGCTAGTG GCTTCGGGGG AGCCCTCTTT 
GTCTACCTGA ACCGGAAGAT TGTCCAGGTG ATGCGGAAGC AAAAAACCAT CAACCGCTTC 
CTCATGAGGA AACGGCTGCT CTTCCCGGCA CTGGTGACTC TGCTCATCTC CACTCTGACC 
TTCCCCCCTG GCTTTGGACA GTTCATGGCC GGACAGCTCT CACAGAAGGA GACCCTAGTC 
ACTCTGTTTG ACAACCGGAC GTGGGTCCGC CAGGGCCTGG TTGAGGATCT AGAGCTACCC 
AGCACTTCAC AGGCCTGGAG CCCACCACGT GCCAATGTCT TCCTTACTCT GGTCATCTTC 
ATCCTCATGA AGTTCTGGAT GTCTGCACTG GCTACCACTA TCCCAGTGCC CTGTGGGGCC 
TTCATGCCTG TCTTTGTCAT TGGAGCGGCA TTTGGGCGGC TGGTGGGCGA AAGCATGGCC 
GCCTGGTTCC CAGATGGGAT TCACACAGAT AGCAGCACCT ACCGAATTGT ACCTGGAGGC 
TATGCTGTGG TCGGGGCGGC TGCACTCGCA GGAGCAGTGA CACACACAGT GTCCACAGCA 
GTGATTGTCT TCGAGCTCAC GGGCCAGATC GCTCACATTC TGCCTGTCAT GATTGCTGTC 
ATCCTGGCTA ATGCTGTTGC CCAGAGCCTG CAGCCATCGC TCTATGACAG TATCATCCGC 
ATCAAGAAGC TGCCCTACCT ACCTGAGCTG GGCTGGGGCC GCCACCAGCA GTACCGGGTG 
CGAGTCGAGG ACATCATGGT TCGGGATGTA CCCCATGTAG CCCTCAGCTG CACTTTTCGG 
GACCTGCGGT TGGCACTGCA CAGAACCAAG GGCCGTATGT TGGCCCTAGT GGAGTCTCCT 
GAGTCCATGA TCCTACTGGG ATCCATCGAA CGCTCACAGG TGGTAGCACT ACTAGGAGCC 
CAGCTGAGCC CAGCGCGCAG GCGGCAGCAC ATGCAAAAGC TAAGAAAAGC CCAGCTGTCT 
CCACCGTCGG ATCAGGAGAG CCCCCCTAGC TCCGAGACAT CTATCCGCTT CCAGGTGAAC 
ACAGAGGACT CGGGCTTCTC TGGAGCCCAC GGGCAGACTC ACAAGCCCCT GAAGCCTGCT 
CTAAAGAGAG GGCCCAGCAA CAGTACAAGC CTGCAGGAAG GTACCACAGG CAACATGGAG 
TCAGCAGGCA TTGCCCTCAG AAGCCTCTTC TGTGGCAGTC CACCTCTGGA GGCAACATCA 
GAATTGGAAA AGTCAGAATC CTGTGACAAG CGCAAGCTGA AGCGGGTCCG AATCTCCCTG 
GCGAGTGACT CAGACCCGGA AGCCGAGATG AGTCCTGAGG AGATCTTAGA GTGGGAAGAA 
CAGCAGCTAG ATGAGCCAGT CAACTTCAGT GACTGCAAAA TCGACCCTGC CCCCTTCCAG 
CTGGTGGAGC GGACTTCTTT GCACAAGACC CACACCATCT TCTCATTGCT GGGAGTGGAC 
CATGCTTATG TCACCAGCAT TGGCAGACTC ATTGGGATTG TCACCCTAAA GGAGCTCCGG 
AAGGCCATTG AAGGCTCTGT CACAGCACAG GGTGTTAAAG TCAGGCCACC CCTCGCCAGT 
TTCCGGGACA GTGCCACCAG CAGCAGTGAC ACAGAGACCA CTGAGGTGCA TGCGCTCTGG 
GGGCCAAGAT CCCGCCACGG CCTCCCACGA GAGGGTACCC CCTCCGACAG TGATGACAAG 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn2 ( XM_030248952.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104812.1 Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X3, mRNA.

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CloneID	OMu51839
Clone ID Related Accession (Same CDS sequence)	XM_006521749.3 , XM_030248952.1 , XM_006521749.4
Accession Version	XM_030248952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	chloride channel protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA GGAGTGGGCT GCTGCTTTGC CGCACCAATC GGAGGAACTA CTGGCGGGGC 
TTCTTTGCGG CCACCTTCAG TGCCTTCATC TTTCGGGTCT TGGCAGTGTG GAACCGTGAT 
GAAGAAACCA TCACAGCTCT CTTCAAAACT CGGTTCCGAC TCGACTTCCC ATTTGACCTG 
CAAGAGCTGC CAGCCTTTGC TGTCATTGGC ATTGCTAGTG GCTTCGGGGG AGCCCTCTTT 
GTCTACCTGA ACCGGAAGAT TGTCCAGGTG ATGCGGAAGC AAAAAACCAT CAACCGCTTC 
CTCATGAGGA AACGGCTGCT CTTCCCGGCA CTGGTGACTC TGCTCATCTC CACTCTGACC 
TTCCCCCCTG GCTTTGGACA GTTCATGGCC GGACAGCTCT CACAGAAGGA GACCCTAGTC 
ACTCTGTTTG ACAACCGGAC GTGGGTCCGC CAGGGCCTGG TTGAGGATCT AGAGCTACCC 
AGCACTTCAC AGGCCTGGAG CCCACCACGT GCCAATGTCT TCCTTACTCT GGTCATCTTC 
ATCCTCATGA AGTTCTGGAT GTCTGCACTG GCTACCACTA TCCCAGTGCC CTGTGGGGCC 
TTCATGCCTG TCTTTGTCAT TGGAGCGGCA TTTGGGCGGC TGGTGGGCGA AAGCATGGCC 
GCCTGGTTCC CAGATGGGAT TCACACAGAT AGCAGCACCT ACCGAATTGT ACCTGGAGGC 
TATGCTGTGG TCGGGGCGGC TGCACTCGCA GGAGCAGTGA CACACACAGT GTCCACAGCA 
GTGATTGTCT TCGAGCTCAC GGGCCAGATC GCTCACATTC TGCCTGTCAT GATTGCTGTC 
ATCCTGGCTA ATGCTGTTGC CCAGAGCCTG CAGCCATCGC TCTATGACAG TATCATCCGC 
ATCAAGAAGC TGCCCTACCT ACCTGAGCTG GGCTGGGGCC GCCACCAGCA GTACCGGGTG 
CGAGTCGAGG ACATCATGGT TCGGGATGTA CCCCATGTAG CCCTCAGCTG CACTTTTCGG 
GACCTGCGGT TGGCACTGCA CAGAACCAAG GGCCGTATGT TGGCCCTAGT GGAGTCTCCT 
GAGTCCATGA TCCTACTGGG ATCCATCGAA CGCTCACAGG TGGTAGCACT ACTAGGAGCC 
CAGCTGAGCC CAGCGCGCAG GCGGCAGCAC ATGCAAAAGC TAAGAAAAGC CCAGCTGTCT 
CCACCGTCGG ATCAGGAGAG CCCCCCTAGC TCCGAGACAT CTATCCGCTT CCAGGTGAAC 
ACAGAGGACT CGGGCTTCTC TGGAGCCCAC GGGCAGACTC ACAAGCCCCT GAAGCCTGCT 
CTAAAGAGAG GGCCCAGCAA CAGTACAAGC CTGCAGGAAG GTACCACAGG CAACATGGAG 
TCAGCAGGCA TTGCCCTCAG AAGCCTCTTC TGTGGCAGTC CACCTCTGGA GGCAACATCA 
GAATTGGAAA AGTCAGAATC CTGTGACAAG CGCAAGCTGA AGCGGGTCCG AATCTCCCTG 
GCGAGTGACT CAGACCCGGA AGCCGAGATG AGTCCTGAGG AGATCTTAGA GTGGGAAGAA 
CAGCAGCTAG ATGAGCCAGT CAACTTCAGT GACTGCAAAA TCGACCCTGC CCCCTTCCAG 
CTGGTGGAGC GGACTTCTTT GCACAAGACC CACACCATCT TCTCATTGCT GGGAGTGGAC 
CATGCTTATG TCACCAGCAT TGGCAGACTC ATTGGGATTG TCACCCTAAA GGAGCTCCGG 
AAGGCCATTG AAGGCTCTGT CACAGCACAG GGTGTTAAAG TCAGGCCACC CCTCGCCAGT 
TTCCGGGACA GTGCCACCAG CAGCAGTGAC ACAGAGACCA CTGAGGTGCA TGCGCTCTGG 
GGGCCAAGAT CCCGCCACGG CCTCCCACGA GAGGGTACCC CCTCCGACAG TGATGACAAG 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104812.1
CDS	1007..2935
Translation	MRSRSGLLLCRTNRRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLELPSTSQAWSPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQLSPPSDQESPPSSETSIRFQVNTEDSGFSGAHGQTHKPLKPALKRGPSNSTSLQEGTTGNMESAGIALRSLFCGSPPLEATSELEKSESCDKRKLKRVRISLASDSDPEAEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ

Target ORF information:

RefSeq Version	XM_030248952.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248952.1

ORF Insert Sequence:

ATGCGCAGTA GGAGTGGGCT GCTGCTTTGC CGCACCAATC GGAGGAACTA CTGGCGGGGC 
TTCTTTGCGG CCACCTTCAG TGCCTTCATC TTTCGGGTCT TGGCAGTGTG GAACCGTGAT 
GAAGAAACCA TCACAGCTCT CTTCAAAACT CGGTTCCGAC TCGACTTCCC ATTTGACCTG 
CAAGAGCTGC CAGCCTTTGC TGTCATTGGC ATTGCTAGTG GCTTCGGGGG AGCCCTCTTT 
GTCTACCTGA ACCGGAAGAT TGTCCAGGTG ATGCGGAAGC AAAAAACCAT CAACCGCTTC 
CTCATGAGGA AACGGCTGCT CTTCCCGGCA CTGGTGACTC TGCTCATCTC CACTCTGACC 
TTCCCCCCTG GCTTTGGACA GTTCATGGCC GGACAGCTCT CACAGAAGGA GACCCTAGTC 
ACTCTGTTTG ACAACCGGAC GTGGGTCCGC CAGGGCCTGG TTGAGGATCT AGAGCTACCC 
AGCACTTCAC AGGCCTGGAG CCCACCACGT GCCAATGTCT TCCTTACTCT GGTCATCTTC 
ATCCTCATGA AGTTCTGGAT GTCTGCACTG GCTACCACTA TCCCAGTGCC CTGTGGGGCC 
TTCATGCCTG TCTTTGTCAT TGGAGCGGCA TTTGGGCGGC TGGTGGGCGA AAGCATGGCC 
GCCTGGTTCC CAGATGGGAT TCACACAGAT AGCAGCACCT ACCGAATTGT ACCTGGAGGC 
TATGCTGTGG TCGGGGCGGC TGCACTCGCA GGAGCAGTGA CACACACAGT GTCCACAGCA 
GTGATTGTCT TCGAGCTCAC GGGCCAGATC GCTCACATTC TGCCTGTCAT GATTGCTGTC 
ATCCTGGCTA ATGCTGTTGC CCAGAGCCTG CAGCCATCGC TCTATGACAG TATCATCCGC 
ATCAAGAAGC TGCCCTACCT ACCTGAGCTG GGCTGGGGCC GCCACCAGCA GTACCGGGTG 
CGAGTCGAGG ACATCATGGT TCGGGATGTA CCCCATGTAG CCCTCAGCTG CACTTTTCGG 
GACCTGCGGT TGGCACTGCA CAGAACCAAG GGCCGTATGT TGGCCCTAGT GGAGTCTCCT 
GAGTCCATGA TCCTACTGGG ATCCATCGAA CGCTCACAGG TGGTAGCACT ACTAGGAGCC 
CAGCTGAGCC CAGCGCGCAG GCGGCAGCAC ATGCAAAAGC TAAGAAAAGC CCAGCTGTCT 
CCACCGTCGG ATCAGGAGAG CCCCCCTAGC TCCGAGACAT CTATCCGCTT CCAGGTGAAC 
ACAGAGGACT CGGGCTTCTC TGGAGCCCAC GGGCAGACTC ACAAGCCCCT GAAGCCTGCT 
CTAAAGAGAG GGCCCAGCAA CAGTACAAGC CTGCAGGAAG GTACCACAGG CAACATGGAG 
TCAGCAGGCA TTGCCCTCAG AAGCCTCTTC TGTGGCAGTC CACCTCTGGA GGCAACATCA 
GAATTGGAAA AGTCAGAATC CTGTGACAAG CGCAAGCTGA AGCGGGTCCG AATCTCCCTG 
GCGAGTGACT CAGACCCGGA AGCCGAGATG AGTCCTGAGG AGATCTTAGA GTGGGAAGAA 
CAGCAGCTAG ATGAGCCAGT CAACTTCAGT GACTGCAAAA TCGACCCTGC CCCCTTCCAG 
CTGGTGGAGC GGACTTCTTT GCACAAGACC CACACCATCT TCTCATTGCT GGGAGTGGAC 
CATGCTTATG TCACCAGCAT TGGCAGACTC ATTGGGATTG TCACCCTAAA GGAGCTCCGG 
AAGGCCATTG AAGGCTCTGT CACAGCACAG GGTGTTAAAG TCAGGCCACC CCTCGCCAGT 
TTCCGGGACA GTGCCACCAG CAGCAGTGAC ACAGAGACCA CTGAGGTGCA TGCGCTCTGG 
GGGCCAAGAT CCCGCCACGG CCTCCCACGA GAGGGTACCC CCTCCGACAG TGATGACAAG 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn2 ( XM_006521749.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521812.1 Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X2, mRNA.

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CloneID	OMu51839
Clone ID Related Accession (Same CDS sequence)	XM_006521749.3 , XM_030248952.1 , XM_006521749.4
Accession Version	XM_006521749.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	chloride channel protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521749.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA GGAGTGGGCT GCTGCTTTGC CGCACCAATC GGAGGAACTA CTGGCGGGGC 
TTCTTTGCGG CCACCTTCAG TGCCTTCATC TTTCGGGTCT TGGCAGTGTG GAACCGTGAT 
GAAGAAACCA TCACAGCTCT CTTCAAAACT CGGTTCCGAC TCGACTTCCC ATTTGACCTG 
CAAGAGCTGC CAGCCTTTGC TGTCATTGGC ATTGCTAGTG GCTTCGGGGG AGCCCTCTTT 
GTCTACCTGA ACCGGAAGAT TGTCCAGGTG ATGCGGAAGC AAAAAACCAT CAACCGCTTC 
CTCATGAGGA AACGGCTGCT CTTCCCGGCA CTGGTGACTC TGCTCATCTC CACTCTGACC 
TTCCCCCCTG GCTTTGGACA GTTCATGGCC GGACAGCTCT CACAGAAGGA GACCCTAGTC 
ACTCTGTTTG ACAACCGGAC GTGGGTCCGC CAGGGCCTGG TTGAGGATCT AGAGCTACCC 
AGCACTTCAC AGGCCTGGAG CCCACCACGT GCCAATGTCT TCCTTACTCT GGTCATCTTC 
ATCCTCATGA AGTTCTGGAT GTCTGCACTG GCTACCACTA TCCCAGTGCC CTGTGGGGCC 
TTCATGCCTG TCTTTGTCAT TGGAGCGGCA TTTGGGCGGC TGGTGGGCGA AAGCATGGCC 
GCCTGGTTCC CAGATGGGAT TCACACAGAT AGCAGCACCT ACCGAATTGT ACCTGGAGGC 
TATGCTGTGG TCGGGGCGGC TGCACTCGCA GGAGCAGTGA CACACACAGT GTCCACAGCA 
GTGATTGTCT TCGAGCTCAC GGGCCAGATC GCTCACATTC TGCCTGTCAT GATTGCTGTC 
ATCCTGGCTA ATGCTGTTGC CCAGAGCCTG CAGCCATCGC TCTATGACAG TATCATCCGC 
ATCAAGAAGC TGCCCTACCT ACCTGAGCTG GGCTGGGGCC GCCACCAGCA GTACCGGGTG 
CGAGTCGAGG ACATCATGGT TCGGGATGTA CCCCATGTAG CCCTCAGCTG CACTTTTCGG 
GACCTGCGGT TGGCACTGCA CAGAACCAAG GGCCGTATGT TGGCCCTAGT GGAGTCTCCT 
GAGTCCATGA TCCTACTGGG ATCCATCGAA CGCTCACAGG TGGTAGCACT ACTAGGAGCC 
CAGCTGAGCC CAGCGCGCAG GCGGCAGCAC ATGCAAAAGC TAAGAAAAGC CCAGCTGTCT 
CCACCGTCGG ATCAGGAGAG CCCCCCTAGC TCCGAGACAT CTATCCGCTT CCAGGTGAAC 
ACAGAGGACT CGGGCTTCTC TGGAGCCCAC GGGCAGACTC ACAAGCCCCT GAAGCCTGCT 
CTAAAGAGAG GGCCCAGCAA CAGTACAAGC CTGCAGGAAG GTACCACAGG CAACATGGAG 
TCAGCAGGCA TTGCCCTCAG AAGCCTCTTC TGTGGCAGTC CACCTCTGGA GGCAACATCA 
GAATTGGAAA AGTCAGAATC CTGTGACAAG CGCAAGCTGA AGCGGGTCCG AATCTCCCTG 
GCGAGTGACT CAGACCCGGA AGCCGAGATG AGTCCTGAGG AGATCTTAGA GTGGGAAGAA 
CAGCAGCTAG ATGAGCCAGT CAACTTCAGT GACTGCAAAA TCGACCCTGC CCCCTTCCAG 
CTGGTGGAGC GGACTTCTTT GCACAAGACC CACACCATCT TCTCATTGCT GGGAGTGGAC 
CATGCTTATG TCACCAGCAT TGGCAGACTC ATTGGGATTG TCACCCTAAA GGAGCTCCGG 
AAGGCCATTG AAGGCTCTGT CACAGCACAG GGTGTTAAAG TCAGGCCACC CCTCGCCAGT 
TTCCGGGACA GTGCCACCAG CAGCAGTGAC ACAGAGACCA CTGAGGTGCA TGCGCTCTGG 
GGGCCAAGAT CCCGCCACGG CCTCCCACGA GAGGGTACCC CCTCCGACAG TGATGACAAG 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521812.1
CDS	2015..3943
Translation	MRSRSGLLLCRTNRRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLELPSTSQAWSPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQLSPPSDQESPPSSETSIRFQVNTEDSGFSGAHGQTHKPLKPALKRGPSNSTSLQEGTTGNMESAGIALRSLFCGSPPLEATSELEKSESCDKRKLKRVRISLASDSDPEAEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ

Target ORF information:

RefSeq Version	XM_006521749.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521749.4

ORF Insert Sequence:

ATGCGCAGTA GGAGTGGGCT GCTGCTTTGC CGCACCAATC GGAGGAACTA CTGGCGGGGC 
TTCTTTGCGG CCACCTTCAG TGCCTTCATC TTTCGGGTCT TGGCAGTGTG GAACCGTGAT 
GAAGAAACCA TCACAGCTCT CTTCAAAACT CGGTTCCGAC TCGACTTCCC ATTTGACCTG 
CAAGAGCTGC CAGCCTTTGC TGTCATTGGC ATTGCTAGTG GCTTCGGGGG AGCCCTCTTT 
GTCTACCTGA ACCGGAAGAT TGTCCAGGTG ATGCGGAAGC AAAAAACCAT CAACCGCTTC 
CTCATGAGGA AACGGCTGCT CTTCCCGGCA CTGGTGACTC TGCTCATCTC CACTCTGACC 
TTCCCCCCTG GCTTTGGACA GTTCATGGCC GGACAGCTCT CACAGAAGGA GACCCTAGTC 
ACTCTGTTTG ACAACCGGAC GTGGGTCCGC CAGGGCCTGG TTGAGGATCT AGAGCTACCC 
AGCACTTCAC AGGCCTGGAG CCCACCACGT GCCAATGTCT TCCTTACTCT GGTCATCTTC 
ATCCTCATGA AGTTCTGGAT GTCTGCACTG GCTACCACTA TCCCAGTGCC CTGTGGGGCC 
TTCATGCCTG TCTTTGTCAT TGGAGCGGCA TTTGGGCGGC TGGTGGGCGA AAGCATGGCC 
GCCTGGTTCC CAGATGGGAT TCACACAGAT AGCAGCACCT ACCGAATTGT ACCTGGAGGC 
TATGCTGTGG TCGGGGCGGC TGCACTCGCA GGAGCAGTGA CACACACAGT GTCCACAGCA 
GTGATTGTCT TCGAGCTCAC GGGCCAGATC GCTCACATTC TGCCTGTCAT GATTGCTGTC 
ATCCTGGCTA ATGCTGTTGC CCAGAGCCTG CAGCCATCGC TCTATGACAG TATCATCCGC 
ATCAAGAAGC TGCCCTACCT ACCTGAGCTG GGCTGGGGCC GCCACCAGCA GTACCGGGTG 
CGAGTCGAGG ACATCATGGT TCGGGATGTA CCCCATGTAG CCCTCAGCTG CACTTTTCGG 
GACCTGCGGT TGGCACTGCA CAGAACCAAG GGCCGTATGT TGGCCCTAGT GGAGTCTCCT 
GAGTCCATGA TCCTACTGGG ATCCATCGAA CGCTCACAGG TGGTAGCACT ACTAGGAGCC 
CAGCTGAGCC CAGCGCGCAG GCGGCAGCAC ATGCAAAAGC TAAGAAAAGC CCAGCTGTCT 
CCACCGTCGG ATCAGGAGAG CCCCCCTAGC TCCGAGACAT CTATCCGCTT CCAGGTGAAC 
ACAGAGGACT CGGGCTTCTC TGGAGCCCAC GGGCAGACTC ACAAGCCCCT GAAGCCTGCT 
CTAAAGAGAG GGCCCAGCAA CAGTACAAGC CTGCAGGAAG GTACCACAGG CAACATGGAG 
TCAGCAGGCA TTGCCCTCAG AAGCCTCTTC TGTGGCAGTC CACCTCTGGA GGCAACATCA 
GAATTGGAAA AGTCAGAATC CTGTGACAAG CGCAAGCTGA AGCGGGTCCG AATCTCCCTG 
GCGAGTGACT CAGACCCGGA AGCCGAGATG AGTCCTGAGG AGATCTTAGA GTGGGAAGAA 
CAGCAGCTAG ATGAGCCAGT CAACTTCAGT GACTGCAAAA TCGACCCTGC CCCCTTCCAG 
CTGGTGGAGC GGACTTCTTT GCACAAGACC CACACCATCT TCTCATTGCT GGGAGTGGAC 
CATGCTTATG TCACCAGCAT TGGCAGACTC ATTGGGATTG TCACCCTAAA GGAGCTCCGG 
AAGGCCATTG AAGGCTCTGT CACAGCACAG GGTGTTAAAG TCAGGCCACC CCTCGCCAGT 
TTCCGGGACA GTGCCACCAG CAGCAGTGAC ACAGAGACCA CTGAGGTGCA TGCGCTCTGG 
GGGCCAAGAT CCCGCCACGG CCTCCCACGA GAGGGTACCC CCTCCGACAG TGATGACAAG 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn2 ( XM_017316862.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172351.1 Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X1, mRNA.

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CloneID	OMu79256
Clone ID Related Accession (Same CDS sequence)	XM_017316862.2 , XM_017316862.1
Accession Version	XM_017316862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	chloride channel protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CAACGGCTGC GGCGGCCGCG GCGGCGGCGG CGGGGGAAGG GATGGAGCCT 
CGAGCGCTGC AGTACGAGCA GACCCTGATG TATGGCCGGT ACACTCAGGA ACTCGGGGCC 
TTTGCCAAAG AGGAAGCTGC TCGTATTCGC CTGGGAGGGC CTGAGCCCTG GAAGGGGTCC 
CCTTCTGCCC GGGCTACCCC AGAGCTCCTA GAATATGGAC AGAGCCGATG TGCCAGATGT 
CGCATTTGTT CTGTACGCTG CCACAAGTTC CTGGTGTCCA GGGTCGGTGA AGACTGGATC 
TTCCTGGTTC TGTTGGGGCT CCTCATGGCA CTGGTCAGCT GGGCTATGGA CTATGCCATC 
GCTGTCTGTC TACAGGGTGA GGGCAAAGGC TGGCGAGCTC AGCAATGGAT GTCCCGGGGC 
TTAAACACCA ACATCTTACT CCAGTACCTG GCTTGGGTTA CCTACCCCGT GGTCCTCATC 
ACTTTCTCTG CTGGATTCAC CCAGATCCTG GCCCCACAGG CTGTCGGGTC TGGCATCCCC 
GAAATGAAAA CCATCCTTCG GGGAGTGGTG CTGAAAGAAT ACCTCACCCT CAAGACCTTT 
GTAGCTAAGG TCATTGGGCT AACCTGTGCC CTGGGCAGTG GGATGCCCCT TGGCAAAGAG 
GGACCCTTTG TGCACATTGC CAGCATGTGT GCCGCCCTTC TCAGCAAGTT CCTCTCCCTC 
TTTGGGGGTA TCTATGAGCA TGAGTCCCGG AACACGGAGA TGCTAGCTGC TGCATGCGCA 
GTAGGAGTGG GCTGCTGCTT TGCCGCACCA ATCGGAGGGG TCCTATTCAG CATTGAAGTC 
ACCTCCACCT TCTTCGCTGT TAGGAACTAC TGGCGGGGCT TCTTTGCGGC CACCTTCAGT 
GCCTTCATCT TTCGGGTCTT GGCAGTGTGG AACCGTGATG AAGAAACCAT CACAGCTCTC 
TTCAAAACTC GGTTCCGACT CGACTTCCCA TTTGACCTGC AAGAGCTGCC AGCCTTTGCT 
GTCATTGGCA TTGCTAGTGG CTTCGGGGGA GCCCTCTTTG TCTACCTGAA CCGGAAGATT 
GTCCAGGTGA TGCGGAAGCA AAAAACCATC AACCGCTTCC TCATGAGGAA ACGGCTGCTC 
TTCCCGGCAC TGGTGACTCT GCTCATCTCC ACTCTGACCT TCCCCCCTGG CTTTGGACAG 
TTCATGGCCG GACAGCTCTC ACAGAAGGAG ACCCTAGTCA CTCTGTTTGA CAACCGGACG 
TGGGTCCGCC AGGGCCTGGT TGAGGATCTA GAGCTACCCA GCACTTCACA GGCCTGGAGC 
CCACCACGTG CCAATGTCTT CCTTACTCTG GTCATCTTCA TCCTCATGAA GTTCTGGATG 
TCTGCACTGG CTACCACTAT CCCAGTGCCC TGTGGGGCCT TCATGCCTGT CTTTGTCATT 
GGAGCGGCAT TTGGGCGGCT GGTGGGCGAA AGCATGGCCG CCTGGTTCCC AGATGGGATT 
CACACAGATA GCAGCACCTA CCGAATTGTA CCTGGAGGCT ATGCTGTGGT CGGGGCGGCT 
GCACTCGCAG GAGCAGTGAC ACACACAGTG TCCACAGCAG TGATTGTCTT CGAGCTCACG 
GGCCAGATCG CTCACATTCT GCCTGTCATG ATTGCTGTCA TCCTGGCTAA TGCTGTTGCC 
CAGAGCCTGC AGCCATCGCT CTATGACAGT ATCATCCGCA TCAAGAAGCT GCCCTACCTA 
CCTGAGCTGG GCTGGGGCCG CCACCAGCAG TACCGGGTGC GAGTCGAGGA CATCATGGTT 
CGGGATGTAC CCCATGTAGC CCTCAGCTGC ACTTTTCGGG ACCTGCGGTT GGCACTGCAC 
AGAACCAAGG GCCGTATGTT GGCCCTAGTG GAGTCTCCTG AGTCCATGAT CCTACTGGGA 
TCCATCGAAC GCTCACAGGT GGTAGCACTA CTAGGAGCCC AGCTGAGCCC AGCGCGCAGG 
CGGCAGCACA TGCAAAAGCT AAGAAAAGCC CAGCTGTCTC CACCGTCGGA TCAGGAGAGC 
CCCCCTAGCT CCGAGACATC TATCCGCTTC CAGGTGAACA CAGAGGACTC GGGCTTCTCT 
GGAGCCCACG GGCAGACTCA CAAGCCCCTG AAGCCTGCTC TAAAGAGAGG GCCCAGCAAC 
AGTACAAGCC TGCAGGAAGG TACCACAGGC AACATGGAGT CAGCAGGCAT TGCCCTCAGA 
AGCCTCTTCT GTGGCAGTCC ACCTCTGGAG GCAACATCAG AATTGGAAAA GTCAGAATCC 
TGTGACAAGC GCAAGCTGAA GCGGGTCCGA ATCTCCCTGG CGAGTGACTC AGACCCGGAA 
GCCGAGATGA GTCCTGAGGA GATCTTAGAG TGGGAAGAAC AGCAGCTAGA TGAGCCAGTC 
AACTTCAGTG ACTGCAAAAT CGACCCTGCC CCCTTCCAGC TGGTGGAGCG GACTTCTTTG 
CACAAGACCC ACACCATCTT CTCATTGCTG GGAGTGGACC ATGCTTATGT CACCAGCATT 
GGCAGACTCA TTGGGATTGT CACCCTAAAG GAGCTCCGGA AGGCCATTGA AGGCTCTGTC 
ACAGCACAGG GTGTTAAAGT CAGGCCACCC CTCGCCAGTT TCCGGGACAG TGCCACCAGC 
AGCAGTGACA CAGAGACCAC TGAGGTGCAT GCGCTCTGGG GGCCAAGATC CCGCCACGGC 
CTCCCACGAG AGGGTACCCC CTCCGACAGT GATGACAAGT GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172351.1
CDS	150..2897
Translation	MAAATAAAAAAAAAGEGMEPRALQYEQTLMYGRYTQELGAFAKEEAARIRLGGPEPWKGSPSARATPELLEYGQSRCARCRICSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWAMDYAIAVCLQGEGKGWRAQQWMSRGLNTNILLQYLAWVTYPVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFVAKVIGLTCALGSGMPLGKEGPFVHIASMCAALLSKFLSLFGGIYEHESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLELPSTSQAWSPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQLSPPSDQESPPSSETSIRFQVNTEDSGFSGAHGQTHKPLKPALKRGPSNSTSLQEGTTGNMESAGIALRSLFCGSPPLEATSELEKSESCDKRKLKRVRISLASDSDPEAEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ

Target ORF information:

RefSeq Version	XM_017316862.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316862.2

ORF Insert Sequence:

ATGGCGGCTG CAACGGCTGC GGCGGCCGCG GCGGCGGCGG CGGGGGAAGG GATGGAGCCT 
CGAGCGCTGC AGTACGAGCA GACCCTGATG TATGGCCGGT ACACTCAGGA ACTCGGGGCC 
TTTGCCAAAG AGGAAGCTGC TCGTATTCGC CTGGGAGGGC CTGAGCCCTG GAAGGGGTCC 
CCTTCTGCCC GGGCTACCCC AGAGCTCCTA GAATATGGAC AGAGCCGATG TGCCAGATGT 
CGCATTTGTT CTGTACGCTG CCACAAGTTC CTGGTGTCCA GGGTCGGTGA AGACTGGATC 
TTCCTGGTTC TGTTGGGGCT CCTCATGGCA CTGGTCAGCT GGGCTATGGA CTATGCCATC 
GCTGTCTGTC TACAGGGTGA GGGCAAAGGC TGGCGAGCTC AGCAATGGAT GTCCCGGGGC 
TTAAACACCA ACATCTTACT CCAGTACCTG GCTTGGGTTA CCTACCCCGT GGTCCTCATC 
ACTTTCTCTG CTGGATTCAC CCAGATCCTG GCCCCACAGG CTGTCGGGTC TGGCATCCCC 
GAAATGAAAA CCATCCTTCG GGGAGTGGTG CTGAAAGAAT ACCTCACCCT CAAGACCTTT 
GTAGCTAAGG TCATTGGGCT AACCTGTGCC CTGGGCAGTG GGATGCCCCT TGGCAAAGAG 
GGACCCTTTG TGCACATTGC CAGCATGTGT GCCGCCCTTC TCAGCAAGTT CCTCTCCCTC 
TTTGGGGGTA TCTATGAGCA TGAGTCCCGG AACACGGAGA TGCTAGCTGC TGCATGCGCA 
GTAGGAGTGG GCTGCTGCTT TGCCGCACCA ATCGGAGGGG TCCTATTCAG CATTGAAGTC 
ACCTCCACCT TCTTCGCTGT TAGGAACTAC TGGCGGGGCT TCTTTGCGGC CACCTTCAGT 
GCCTTCATCT TTCGGGTCTT GGCAGTGTGG AACCGTGATG AAGAAACCAT CACAGCTCTC 
TTCAAAACTC GGTTCCGACT CGACTTCCCA TTTGACCTGC AAGAGCTGCC AGCCTTTGCT 
GTCATTGGCA TTGCTAGTGG CTTCGGGGGA GCCCTCTTTG TCTACCTGAA CCGGAAGATT 
GTCCAGGTGA TGCGGAAGCA AAAAACCATC AACCGCTTCC TCATGAGGAA ACGGCTGCTC 
TTCCCGGCAC TGGTGACTCT GCTCATCTCC ACTCTGACCT TCCCCCCTGG CTTTGGACAG 
TTCATGGCCG GACAGCTCTC ACAGAAGGAG ACCCTAGTCA CTCTGTTTGA CAACCGGACG 
TGGGTCCGCC AGGGCCTGGT TGAGGATCTA GAGCTACCCA GCACTTCACA GGCCTGGAGC 
CCACCACGTG CCAATGTCTT CCTTACTCTG GTCATCTTCA TCCTCATGAA GTTCTGGATG 
TCTGCACTGG CTACCACTAT CCCAGTGCCC TGTGGGGCCT TCATGCCTGT CTTTGTCATT 
GGAGCGGCAT TTGGGCGGCT GGTGGGCGAA AGCATGGCCG CCTGGTTCCC AGATGGGATT 
CACACAGATA GCAGCACCTA CCGAATTGTA CCTGGAGGCT ATGCTGTGGT CGGGGCGGCT 
GCACTCGCAG GAGCAGTGAC ACACACAGTG TCCACAGCAG TGATTGTCTT CGAGCTCACG 
GGCCAGATCG CTCACATTCT GCCTGTCATG ATTGCTGTCA TCCTGGCTAA TGCTGTTGCC 
CAGAGCCTGC AGCCATCGCT CTATGACAGT ATCATCCGCA TCAAGAAGCT GCCCTACCTA 
CCTGAGCTGG GCTGGGGCCG CCACCAGCAG TACCGGGTGC GAGTCGAGGA CATCATGGTT 
CGGGATGTAC CCCATGTAGC CCTCAGCTGC ACTTTTCGGG ACCTGCGGTT GGCACTGCAC 
AGAACCAAGG GCCGTATGTT GGCCCTAGTG GAGTCTCCTG AGTCCATGAT CCTACTGGGA 
TCCATCGAAC GCTCACAGGT GGTAGCACTA CTAGGAGCCC AGCTGAGCCC AGCGCGCAGG 
CGGCAGCACA TGCAAAAGCT AAGAAAAGCC CAGCTGTCTC CACCGTCGGA TCAGGAGAGC 
CCCCCTAGCT CCGAGACATC TATCCGCTTC CAGGTGAACA CAGAGGACTC GGGCTTCTCT 
GGAGCCCACG GGCAGACTCA CAAGCCCCTG AAGCCTGCTC TAAAGAGAGG GCCCAGCAAC 
AGTACAAGCC TGCAGGAAGG TACCACAGGC AACATGGAGT CAGCAGGCAT TGCCCTCAGA 
AGCCTCTTCT GTGGCAGTCC ACCTCTGGAG GCAACATCAG AATTGGAAAA GTCAGAATCC 
TGTGACAAGC GCAAGCTGAA GCGGGTCCGA ATCTCCCTGG CGAGTGACTC AGACCCGGAA 
GCCGAGATGA GTCCTGAGGA GATCTTAGAG TGGGAAGAAC AGCAGCTAGA TGAGCCAGTC 
AACTTCAGTG ACTGCAAAAT CGACCCTGCC CCCTTCCAGC TGGTGGAGCG GACTTCTTTG 
CACAAGACCC ACACCATCTT CTCATTGCTG GGAGTGGACC ATGCTTATGT CACCAGCATT 
GGCAGACTCA TTGGGATTGT CACCCTAAAG GAGCTCCGGA AGGCCATTGA AGGCTCTGTC 
ACAGCACAGG GTGTTAAAGT CAGGCCACCC CTCGCCAGTT TCCGGGACAG TGCCACCAGC 
AGCAGTGACA CAGAGACCAC TGAGGTGCAT GCGCTCTGGG GGCCAAGATC CCGCCACGGC 
CTCCCACGAG AGGGTACCCC CTCCGACAGT GATGACAAGT GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcn2 ( XM_017316862.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172351.1 Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X1, mRNA.

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CloneID	OMu79256
Clone ID Related Accession (Same CDS sequence)	XM_017316862.2 , XM_017316862.1
Accession Version	XM_017316862.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	chloride channel protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CAACGGCTGC GGCGGCCGCG GCGGCGGCGG CGGGGGAAGG GATGGAGCCT 
CGAGCGCTGC AGTACGAGCA GACCCTGATG TATGGCCGGT ACACTCAGGA ACTCGGGGCC 
TTTGCCAAAG AGGAAGCTGC TCGTATTCGC CTGGGAGGGC CTGAGCCCTG GAAGGGGTCC 
CCTTCTGCCC GGGCTACCCC AGAGCTCCTA GAATATGGAC AGAGCCGATG TGCCAGATGT 
CGCATTTGTT CTGTACGCTG CCACAAGTTC CTGGTGTCCA GGGTCGGTGA AGACTGGATC 
TTCCTGGTTC TGTTGGGGCT CCTCATGGCA CTGGTCAGCT GGGCTATGGA CTATGCCATC 
GCTGTCTGTC TACAGGGTGA GGGCAAAGGC TGGCGAGCTC AGCAATGGAT GTCCCGGGGC 
TTAAACACCA ACATCTTACT CCAGTACCTG GCTTGGGTTA CCTACCCCGT GGTCCTCATC 
ACTTTCTCTG CTGGATTCAC CCAGATCCTG GCCCCACAGG CTGTCGGGTC TGGCATCCCC 
GAAATGAAAA CCATCCTTCG GGGAGTGGTG CTGAAAGAAT ACCTCACCCT CAAGACCTTT 
GTAGCTAAGG TCATTGGGCT AACCTGTGCC CTGGGCAGTG GGATGCCCCT TGGCAAAGAG 
GGACCCTTTG TGCACATTGC CAGCATGTGT GCCGCCCTTC TCAGCAAGTT CCTCTCCCTC 
TTTGGGGGTA TCTATGAGCA TGAGTCCCGG AACACGGAGA TGCTAGCTGC TGCATGCGCA 
GTAGGAGTGG GCTGCTGCTT TGCCGCACCA ATCGGAGGGG TCCTATTCAG CATTGAAGTC 
ACCTCCACCT TCTTCGCTGT TAGGAACTAC TGGCGGGGCT TCTTTGCGGC CACCTTCAGT 
GCCTTCATCT TTCGGGTCTT GGCAGTGTGG AACCGTGATG AAGAAACCAT CACAGCTCTC 
TTCAAAACTC GGTTCCGACT CGACTTCCCA TTTGACCTGC AAGAGCTGCC AGCCTTTGCT 
GTCATTGGCA TTGCTAGTGG CTTCGGGGGA GCCCTCTTTG TCTACCTGAA CCGGAAGATT 
GTCCAGGTGA TGCGGAAGCA AAAAACCATC AACCGCTTCC TCATGAGGAA ACGGCTGCTC 
TTCCCGGCAC TGGTGACTCT GCTCATCTCC ACTCTGACCT TCCCCCCTGG CTTTGGACAG 
TTCATGGCCG GACAGCTCTC ACAGAAGGAG ACCCTAGTCA CTCTGTTTGA CAACCGGACG 
TGGGTCCGCC AGGGCCTGGT TGAGGATCTA GAGCTACCCA GCACTTCACA GGCCTGGAGC 
CCACCACGTG CCAATGTCTT CCTTACTCTG GTCATCTTCA TCCTCATGAA GTTCTGGATG 
TCTGCACTGG CTACCACTAT CCCAGTGCCC TGTGGGGCCT TCATGCCTGT CTTTGTCATT 
GGAGCGGCAT TTGGGCGGCT GGTGGGCGAA AGCATGGCCG CCTGGTTCCC AGATGGGATT 
CACACAGATA GCAGCACCTA CCGAATTGTA CCTGGAGGCT ATGCTGTGGT CGGGGCGGCT 
GCACTCGCAG GAGCAGTGAC ACACACAGTG TCCACAGCAG TGATTGTCTT CGAGCTCACG 
GGCCAGATCG CTCACATTCT GCCTGTCATG ATTGCTGTCA TCCTGGCTAA TGCTGTTGCC 
CAGAGCCTGC AGCCATCGCT CTATGACAGT ATCATCCGCA TCAAGAAGCT GCCCTACCTA 
CCTGAGCTGG GCTGGGGCCG CCACCAGCAG TACCGGGTGC GAGTCGAGGA CATCATGGTT 
CGGGATGTAC CCCATGTAGC CCTCAGCTGC ACTTTTCGGG ACCTGCGGTT GGCACTGCAC 
AGAACCAAGG GCCGTATGTT GGCCCTAGTG GAGTCTCCTG AGTCCATGAT CCTACTGGGA 
TCCATCGAAC GCTCACAGGT GGTAGCACTA CTAGGAGCCC AGCTGAGCCC AGCGCGCAGG 
CGGCAGCACA TGCAAAAGCT AAGAAAAGCC CAGCTGTCTC CACCGTCGGA TCAGGAGAGC 
CCCCCTAGCT CCGAGACATC TATCCGCTTC CAGGTGAACA CAGAGGACTC GGGCTTCTCT 
GGAGCCCACG GGCAGACTCA CAAGCCCCTG AAGCCTGCTC TAAAGAGAGG GCCCAGCAAC 
AGTACAAGCC TGCAGGAAGG TACCACAGGC AACATGGAGT CAGCAGGCAT TGCCCTCAGA 
AGCCTCTTCT GTGGCAGTCC ACCTCTGGAG GCAACATCAG AATTGGAAAA GTCAGAATCC 
TGTGACAAGC GCAAGCTGAA GCGGGTCCGA ATCTCCCTGG CGAGTGACTC AGACCCGGAA 
GCCGAGATGA GTCCTGAGGA GATCTTAGAG TGGGAAGAAC AGCAGCTAGA TGAGCCAGTC 
AACTTCAGTG ACTGCAAAAT CGACCCTGCC CCCTTCCAGC TGGTGGAGCG GACTTCTTTG 
CACAAGACCC ACACCATCTT CTCATTGCTG GGAGTGGACC ATGCTTATGT CACCAGCATT 
GGCAGACTCA TTGGGATTGT CACCCTAAAG GAGCTCCGGA AGGCCATTGA AGGCTCTGTC 
ACAGCACAGG GTGTTAAAGT CAGGCCACCC CTCGCCAGTT TCCGGGACAG TGCCACCAGC 
AGCAGTGACA CAGAGACCAC TGAGGTGCAT GCGCTCTGGG GGCCAAGATC CCGCCACGGC 
CTCCCACGAG AGGGTACCCC CTCCGACAGT GATGACAAGT GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172351.1
CDS	718..3465
Translation	MAAATAAAAAAAAAGEGMEPRALQYEQTLMYGRYTQELGAFAKEEAARIRLGGPEPWKGSPSARATPELLEYGQSRCARCRICSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWAMDYAIAVCLQGEGKGWRAQQWMSRGLNTNILLQYLAWVTYPVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFVAKVIGLTCALGSGMPLGKEGPFVHIASMCAALLSKFLSLFGGIYEHESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLELPSTSQAWSPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQLSPPSDQESPPSSETSIRFQVNTEDSGFSGAHGQTHKPLKPALKRGPSNSTSLQEGTTGNMESAGIALRSLFCGSPPLEATSELEKSESCDKRKLKRVRISLASDSDPEAEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKCQ

Target ORF information:

RefSeq Version	XM_017316862.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive 2 (Clcn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316862.1

ORF Insert Sequence:

ATGGCGGCTG CAACGGCTGC GGCGGCCGCG GCGGCGGCGG CGGGGGAAGG GATGGAGCCT 
CGAGCGCTGC AGTACGAGCA GACCCTGATG TATGGCCGGT ACACTCAGGA ACTCGGGGCC 
TTTGCCAAAG AGGAAGCTGC TCGTATTCGC CTGGGAGGGC CTGAGCCCTG GAAGGGGTCC 
CCTTCTGCCC GGGCTACCCC AGAGCTCCTA GAATATGGAC AGAGCCGATG TGCCAGATGT 
CGCATTTGTT CTGTACGCTG CCACAAGTTC CTGGTGTCCA GGGTCGGTGA AGACTGGATC 
TTCCTGGTTC TGTTGGGGCT CCTCATGGCA CTGGTCAGCT GGGCTATGGA CTATGCCATC 
GCTGTCTGTC TACAGGGTGA GGGCAAAGGC TGGCGAGCTC AGCAATGGAT GTCCCGGGGC 
TTAAACACCA ACATCTTACT CCAGTACCTG GCTTGGGTTA CCTACCCCGT GGTCCTCATC 
ACTTTCTCTG CTGGATTCAC CCAGATCCTG GCCCCACAGG CTGTCGGGTC TGGCATCCCC 
GAAATGAAAA CCATCCTTCG GGGAGTGGTG CTGAAAGAAT ACCTCACCCT CAAGACCTTT 
GTAGCTAAGG TCATTGGGCT AACCTGTGCC CTGGGCAGTG GGATGCCCCT TGGCAAAGAG 
GGACCCTTTG TGCACATTGC CAGCATGTGT GCCGCCCTTC TCAGCAAGTT CCTCTCCCTC 
TTTGGGGGTA TCTATGAGCA TGAGTCCCGG AACACGGAGA TGCTAGCTGC TGCATGCGCA 
GTAGGAGTGG GCTGCTGCTT TGCCGCACCA ATCGGAGGGG TCCTATTCAG CATTGAAGTC 
ACCTCCACCT TCTTCGCTGT TAGGAACTAC TGGCGGGGCT TCTTTGCGGC CACCTTCAGT 
GCCTTCATCT TTCGGGTCTT GGCAGTGTGG AACCGTGATG AAGAAACCAT CACAGCTCTC 
TTCAAAACTC GGTTCCGACT CGACTTCCCA TTTGACCTGC AAGAGCTGCC AGCCTTTGCT 
GTCATTGGCA TTGCTAGTGG CTTCGGGGGA GCCCTCTTTG TCTACCTGAA CCGGAAGATT 
GTCCAGGTGA TGCGGAAGCA AAAAACCATC AACCGCTTCC TCATGAGGAA ACGGCTGCTC 
TTCCCGGCAC TGGTGACTCT GCTCATCTCC ACTCTGACCT TCCCCCCTGG CTTTGGACAG 
TTCATGGCCG GACAGCTCTC ACAGAAGGAG ACCCTAGTCA CTCTGTTTGA CAACCGGACG 
TGGGTCCGCC AGGGCCTGGT TGAGGATCTA GAGCTACCCA GCACTTCACA GGCCTGGAGC 
CCACCACGTG CCAATGTCTT CCTTACTCTG GTCATCTTCA TCCTCATGAA GTTCTGGATG 
TCTGCACTGG CTACCACTAT CCCAGTGCCC TGTGGGGCCT TCATGCCTGT CTTTGTCATT 
GGAGCGGCAT TTGGGCGGCT GGTGGGCGAA AGCATGGCCG CCTGGTTCCC AGATGGGATT 
CACACAGATA GCAGCACCTA CCGAATTGTA CCTGGAGGCT ATGCTGTGGT CGGGGCGGCT 
GCACTCGCAG GAGCAGTGAC ACACACAGTG TCCACAGCAG TGATTGTCTT CGAGCTCACG 
GGCCAGATCG CTCACATTCT GCCTGTCATG ATTGCTGTCA TCCTGGCTAA TGCTGTTGCC 
CAGAGCCTGC AGCCATCGCT CTATGACAGT ATCATCCGCA TCAAGAAGCT GCCCTACCTA 
CCTGAGCTGG GCTGGGGCCG CCACCAGCAG TACCGGGTGC GAGTCGAGGA CATCATGGTT 
CGGGATGTAC CCCATGTAGC CCTCAGCTGC ACTTTTCGGG ACCTGCGGTT GGCACTGCAC 
AGAACCAAGG GCCGTATGTT GGCCCTAGTG GAGTCTCCTG AGTCCATGAT CCTACTGGGA 
TCCATCGAAC GCTCACAGGT GGTAGCACTA CTAGGAGCCC AGCTGAGCCC AGCGCGCAGG 
CGGCAGCACA TGCAAAAGCT AAGAAAAGCC CAGCTGTCTC CACCGTCGGA TCAGGAGAGC 
CCCCCTAGCT CCGAGACATC TATCCGCTTC CAGGTGAACA CAGAGGACTC GGGCTTCTCT 
GGAGCCCACG GGCAGACTCA CAAGCCCCTG AAGCCTGCTC TAAAGAGAGG GCCCAGCAAC 
AGTACAAGCC TGCAGGAAGG TACCACAGGC AACATGGAGT CAGCAGGCAT TGCCCTCAGA 
AGCCTCTTCT GTGGCAGTCC ACCTCTGGAG GCAACATCAG AATTGGAAAA GTCAGAATCC 
TGTGACAAGC GCAAGCTGAA GCGGGTCCGA ATCTCCCTGG CGAGTGACTC AGACCCGGAA 
GCCGAGATGA GTCCTGAGGA GATCTTAGAG TGGGAAGAAC AGCAGCTAGA TGAGCCAGTC 
AACTTCAGTG ACTGCAAAAT CGACCCTGCC CCCTTCCAGC TGGTGGAGCG GACTTCTTTG 
CACAAGACCC ACACCATCTT CTCATTGCTG GGAGTGGACC ATGCTTATGT CACCAGCATT 
GGCAGACTCA TTGGGATTGT CACCCTAAAG GAGCTCCGGA AGGCCATTGA AGGCTCTGTC 
ACAGCACAGG GTGTTAAAGT CAGGCCACCC CTCGCCAGTT TCCGGGACAG TGCCACCAGC 
AGCAGTGACA CAGAGACCAC TGAGGTGCAT GCGCTCTGGG GGCCAAGATC CCGCCACGGC 
CTCCCACGAG AGGGTACCCC CTCCGACAGT GATGACAAGT GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathogenesis of hypertension in a mouse model for human CLCN2 related hyperaldosteronism.
Nature communications10(1)4678(2019 10)
Göppner C,Orozco IJ,Hoegg-Beiler MB,Soria AH,Hübner CA,Fernandes-Rosa FL,Boulkroun S,Zennaro MC,Jentsch TJ

Knockout of ClC-2 reveals critical functions of adherens junctions in colonic homeostasis and tumorigenicity.
American journal of physiology. Gastrointestinal and liver physiology315(6)G966-G979(2018 12)
Jin Y,Ibrahim D,Magness ST,Blikslager AT

A gain-of-function mutation in the CLCN2 chloride channel gene causes primary aldosteronism.
Nature genetics50(3)355-361(2018 03)
Fernandes-Rosa FL,Daniil G,Orozco IJ,Göppner C,El Zein R,Jain V,Boulkroun S,Jeunemaitre X,Amar L,Lefebvre H,Schwarzmayr T,Strom TM,Jentsch TJ,Zennaro MC

Lack of ClC-2 Alleviates High Fat Diet-Induced Insulin Resistance and Non-Alcoholic Fatty Liver Disease.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology45(6)2187-2198(2018)
Fu D,Cui H,Zhang Y

Mouse models of human ocular disease for translational research.
PloS one12(8)e0183837(2017)
Krebs MP,Collin GB,Hicks WL,Yu M,Charette JR,Shi LY,Wang J,Naggert JK,Peachey NS,Nishina PM

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Clcn2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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