Ano1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ano1
Entrez Gene ID	101772
Full Name	anoctamin 1, calcium activated chloride channel
Synonyms	Tmem16a
General protein information	Preferred Names anoctamin 1, calcium activated chloride channel Names anoctamin-1 transmembrane protein 16A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006508458.4	XP_006508521.1	anoctamin-1 isoform X6
XM_006508459.4	XP_006508522.1	anoctamin-1 isoform X8
XM_030241952.1	XP_030097812.1	anoctamin-1 isoform X1
XM_006508459.3	XP_006508522.1	anoctamin-1 isoform X4
XM_006508464.2	XP_006508527.1	anoctamin-1 isoform X7
XM_011241975.3	XP_011240277.2	anoctamin-1 isoform X9
XM_011241976.2	XP_011240278.1	anoctamin-1 isoform X17
XM_030241956.1	XP_030097816.1	anoctamin-1 isoform X10
XM_030241960.1	XP_030097820.1	anoctamin-1 isoform X14
XM_011241973.2	XP_011240275.1	anoctamin-1 isoform X1
XM_011241974.2	XP_011240276.1	anoctamin-1 isoform X2
XM_011241974.3	XP_011240276.2	anoctamin-1 isoform X4
XM_011241975.2	XP_011240277.1	anoctamin-1 isoform X5
XM_006508464.3	XP_006508527.1	anoctamin-1 isoform X16
XM_017321913.1	XP_017177402.1	anoctamin-1 isoform X9
NM_178642.5	NP_848757.4	anoctamin-1 isoform 1
XM_030241955.1	XP_030097815.1	anoctamin-1 isoform X7
XM_030241959.1	XP_030097819.1	anoctamin-1 isoform X13
XM_011241973.3	XP_011240275.2	anoctamin-1 isoform X2
NM_001242349.2	NP_001229278.2	anoctamin-1 isoform 2
XM_006508463.4	XP_006508526.1	anoctamin-1 isoform X15
NM_178642.6	NP_848757.5	anoctamin-1 isoform 1
XM_030241958.1	XP_030097818.1	anoctamin-1 isoform X12
XM_030241953.1	XP_030097813.1	anoctamin-1 isoform X3
XM_030241954.1	XP_030097814.1	anoctamin-1 isoform X5
XM_030241957.1	XP_030097817.1	anoctamin-1 isoform X11
NM_001242349.1	NP_001229278.1	anoctamin-1 isoform 2
XM_006508458.3	XP_006508521.1	anoctamin-1 isoform X3
XM_006508463.3	XP_006508526.1	anoctamin-1 isoform X6
XM_017321913.2	XP_017177402.1	anoctamin-1 isoform X18

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANO1	XP_854489.4
Rattus norvegicus (Norway rat)	Ano1	NP_001101034.1
Gallus gallus (chicken)	ANO1	XP_004941586.1
Pan troglodytes (chimpanzee)	ANO1	XP_003313240.1
Mus musculus (house mouse)	Ano1	NP_848757.4
Homo sapiens (human)	ANO1	NP_060513.5
Danio rerio (zebrafish)	ano1	NP_001155062.1
Macaca mulatta (Rhesus monkey)	LOC708723	XP_002799504.1
Bos taurus (cattle)	ANO1	NP_001179646.1
Drosophila melanogaster (fruit fly)	CG16718	NP_001262736.1
Xenopus tropicalis (tropical clawed frog)	ano1	NP_001123799.1

Gene Ontology
Bibliography

Related articles in PubMed

ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain.
Hong GS, Lee SH, Lee B, Choi JH, Oh SJ, Jang Y, Hwang EM, Kim H, Jung J, Kim IB, Oh U
Proceedings of the National Academy of Sciences of the United States of America116(25)12494-12499(2019 Jun)

Nephron-specific knockout of TMEM16A leads to reduced number of glomeruli and albuminuria.
Schenk LK, Buchholz B, Henke SF, Michgehl U, Daniel C, Amann K, Kunzelmann K, Pavenstädt H
American journal of physiology. Renal physiology315(6)F1777-F1786(2018 12)

Calcium-dependent electrostatic control of anion access to the pore of the calcium-activated chloride channel TMEM16A.
Lam AK, Dutzler R
eLife7(2018 10)

Transmembrane member 16A participates in hydrogen peroxide-induced apoptosis by facilitating mitochondria-dependent pathway in vascular smooth muscle cells.
Zeng JW, Chen BY, Lv XF, Sun L, Zeng XL, Zheng HQ, Du YH, Wang GL, Ma MM, Guan YY
British journal of pharmacology175(18)3669-3684(2018 09)

Tissue-specific variation in 5'-terminal exons of mouse Anoctamin 1 transcript induces N-terminal variation of its protein via alternative translational start sites.
Kamikawa A, Sakazaki J, Ichii O
Biochemical and biophysical research communications503(3)1710-1715(2018 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ano1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ano1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu90663	NM_001242349.2 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu90664	NM_178642.6 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu72037	XM_011241975.2 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu72038	XM_011241976.2 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu22943	NM_178642.5 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu23048	NM_001242349.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40133	XM_006508458.4 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40134	XM_006508459.4 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40138	XM_006508463.4 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40139	XM_006508464.3 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72035	XM_011241973.2 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu81888	XM_017321913.2 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89309	XM_030241952.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89310	XM_011241973.3 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89311	XM_030241953.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89312	XM_011241974.3 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89313	XM_030241954.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89314	XM_030241955.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89315	XM_011241975.3 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89316	XM_030241956.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89317	XM_030241957.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89318	XM_030241958.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89319	XM_030241959.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu89320	XM_030241960.1 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72036	XM_011241974.2 Latest version!	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$510.30 $729.00
View Old Accession Versions >>
OMu40134	XM_006508459.3	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40139	XM_006508464.2	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40133	XM_006508458.3	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40138	XM_006508463.3	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu81888	XM_017321913.1	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ano1 ( NM_001242349.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001229278.2 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu90663
Clone ID Related Accession (Same CDS sequence)	NM_001242349.2
Accession Version	NM_001242349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575907200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122336.4 and AC153792.4. On Dec 11, 2019 this sequence version replaced NM_001242349.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062959.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGATCATCCC AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG 
AAGTCACTGA GAAAAGAATC CAGAAACAAA GAGACCGACA AGGTGAAGCT GACCTGGAGG 
GACCGATTCC CAGCCTATTT CACCAATCTT GTCTCCATCA TCTTCATGAT CGCAGTGACA 
TTTGCAATCG TCCTCGGAGT TATCATCTAT AGAATCTCCA CAGCTGCAGC CTTGGCCATG 
AACTCCTCCC CGTCTGTGCG GTCCAACATC CGGGTTACAG TCACGGCCAC CGCTGTTATC 
ATCAACCTCG TGGTCATCAT TCTGCTGGAT GAAGTTTACG GCTGCATTGC CAGGTGGCTC 
ACCAAGATTG AGGTCCCAAA GACAGAGAAG AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC 
TTCCTGCTCA AGTTTGTGAA CTCTTACACT CCCATCTTCT ATGTCGCCTT CTTCAAAGGC 
CGGTTTGTTG GTCGGCCCGG TGACTACGTG TACATCTTCC GCTCTTTCCG GATGGAGGAG 
TGTGCCCCGG GCGGCTGCCT CATGGAGCTC TGTATCCAGC TGAGCATCAT TATGCTGGGC 
AAGCAGCTAA TCCAGAACAA TCTCTTCGAG ATTGGCATCC CGAAGATGAA AAAGTTCATC 
CGCTACCTGA AGCTGCGCAG ACAGAGCCCC TCAGACCGTG AAGAGTACGT GAAGCGGAAG 
CAGCGCTATG AGGTGGACTT CAACCTCGAA CCTTTCGCCG GCCTCACGCC CGAGTACATG 
GAAATGATCA TTCAGTTCGG CTTTGTCACC CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA 
CTCTTCGCCC TGCTAAACAA CATCATTGAG ATCCGCCTGG ATGCCAAAAA GTTTGTCACC 
GAGCTACGGA GGCCAGTAGC CATCAGAGCC AAAGACATCG GCATCTGGTA TAACATCCTC 
AGAGGTGTTG GGAAGCTGGC TGTCATCATT AATGCCTTTG TGATCTCCTT CACGTCTGAC 
TTCATCCCTC GCCTGGTGTA CCTCTACATG TACAGTCAGA ATGGGACCAT GCACGGCTTC 
GTCAATCACA CGCTCTCTTC CTTCAATGTC AGCGACTTCC AGAATGGCAC AGCACCCAAT 
GACCCACTGG ACCTGGGCTA TGAGGTTCAG ATCTGCAGGT ATAAAGATTA CCGGGAACCC 
CCATGGTCAG AACACAAGTA TGACATCTCC AAAGACTTCT GGGCTGTCCT GGCCGCCCGA 
CTGGCATTTG TCATTGTCTT CCAGAACCTG GTGATGTTCA TGAGTGACTT TGTGGACTGG 
GTGATCCCTG ATATCCCCAA AGACATCAGC CAGCAGATCC ACAAAGAGAA GGTTCTCATG 
GTGGAGCTGT TTATGCGTGA GGAGCAGGGC AAGCAGCAGC TACTGGACAC ATGGATGGAG 
AAGGAGAAGC CAAGGGATGT GCCTTGTAAC AACCACAGCC CCACAACCCA CCCAGAGGCA 
GGCGACGGCA GCCCAGTCCC CAGCTACGAG TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229278.2
CDS	253..3123
Translation	MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	NM_001242349.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242349.2

ORF Insert Sequence:

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGATCATCCC AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG 
AAGTCACTGA GAAAAGAATC CAGAAACAAA GAGACCGACA AGGTGAAGCT GACCTGGAGG 
GACCGATTCC CAGCCTATTT CACCAATCTT GTCTCCATCA TCTTCATGAT CGCAGTGACA 
TTTGCAATCG TCCTCGGAGT TATCATCTAT AGAATCTCCA CAGCTGCAGC CTTGGCCATG 
AACTCCTCCC CGTCTGTGCG GTCCAACATC CGGGTTACAG TCACGGCCAC CGCTGTTATC 
ATCAACCTCG TGGTCATCAT TCTGCTGGAT GAAGTTTACG GCTGCATTGC CAGGTGGCTC 
ACCAAGATTG AGGTCCCAAA GACAGAGAAG AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC 
TTCCTGCTCA AGTTTGTGAA CTCTTACACT CCCATCTTCT ATGTCGCCTT CTTCAAAGGC 
CGGTTTGTTG GTCGGCCCGG TGACTACGTG TACATCTTCC GCTCTTTCCG GATGGAGGAG 
TGTGCCCCGG GCGGCTGCCT CATGGAGCTC TGTATCCAGC TGAGCATCAT TATGCTGGGC 
AAGCAGCTAA TCCAGAACAA TCTCTTCGAG ATTGGCATCC CGAAGATGAA AAAGTTCATC 
CGCTACCTGA AGCTGCGCAG ACAGAGCCCC TCAGACCGTG AAGAGTACGT GAAGCGGAAG 
CAGCGCTATG AGGTGGACTT CAACCTCGAA CCTTTCGCCG GCCTCACGCC CGAGTACATG 
GAAATGATCA TTCAGTTCGG CTTTGTCACC CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA 
CTCTTCGCCC TGCTAAACAA CATCATTGAG ATCCGCCTGG ATGCCAAAAA GTTTGTCACC 
GAGCTACGGA GGCCAGTAGC CATCAGAGCC AAAGACATCG GCATCTGGTA TAACATCCTC 
AGAGGTGTTG GGAAGCTGGC TGTCATCATT AATGCCTTTG TGATCTCCTT CACGTCTGAC 
TTCATCCCTC GCCTGGTGTA CCTCTACATG TACAGTCAGA ATGGGACCAT GCACGGCTTC 
GTCAATCACA CGCTCTCTTC CTTCAATGTC AGCGACTTCC AGAATGGCAC AGCACCCAAT 
GACCCACTGG ACCTGGGCTA TGAGGTTCAG ATCTGCAGGT ATAAAGATTA CCGGGAACCC 
CCATGGTCAG AACACAAGTA TGACATCTCC AAAGACTTCT GGGCTGTCCT GGCCGCCCGA 
CTGGCATTTG TCATTGTCTT CCAGAACCTG GTGATGTTCA TGAGTGACTT TGTGGACTGG 
GTGATCCCTG ATATCCCCAA AGACATCAGC CAGCAGATCC ACAAAGAGAA GGTTCTCATG 
GTGGAGCTGT TTATGCGTGA GGAGCAGGGC AAGCAGCAGC TACTGGACAC ATGGATGGAG 
AAGGAGAAGC CAAGGGATGT GCCTTGTAAC AACCACAGCC CCACAACCCA CCCAGAGGCA 
GGCGACGGCA GCCCAGTCCC CAGCTACGAG TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( NM_178642.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848757.5 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 1, mRNA.

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CloneID	OMu90664
Clone ID Related Accession (Same CDS sequence)	NM_178642.6
Accession Version	NM_178642.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575907200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122336.4 and AC153792.4. On Dec 11, 2019 this sequence version replaced NM_178642.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052589.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848757.5
CDS	250..3132
Translation	MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	NM_178642.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178642.6

ORF Insert Sequence:

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_011241975.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240277.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu72037
Clone ID Related Accession (Same CDS sequence)	XM_011241975.2
Accession Version	XM_011241975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA CCGACAAGGT GAAGCTGACC 
TGGAGGGACC GATTCCCAGC CTATTTCACC AATCTTGTCT CCATCATCTT CATGATCGCA 
GTGACATTTG CAATCGTCCT CGGAGTTATC ATCTATAGAA TCTCCACAGC TGCAGCCTTG 
GCCATGAACT CCTCCCCGTC TGTGCGGTCC AACATCCGGG TTACAGTCAC GGCCACCGCT 
GTTATCATCA ACCTCGTGGT CATCATTCTG CTGGATGAAG TTTACGGCTG CATTGCCAGG 
TGGCTCACCA AGATTGAGGT CCCAAAGACA GAGAAGAGCT TTGAGGAGAG GCTAACCTTC 
AAGGCCTTCC TGCTCAAGTT TGTGAACTCT TACACTCCCA TCTTCTATGT CGCCTTCTTC 
AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC TACGTGTACA TCTTCCGCTC TTTCCGGATG 
GAGGAGTGTG CCCCGGGCGG CTGCCTCATG GAGCTCTGTA TCCAGCTGAG CATCATTATG 
CTGGGCAAGC AGCTAATCCA GAACAATCTC TTCGAGATTG GCATCCCGAA GATGAAAAAG 
TTCATCCGCT ACCTGAAGCT GCGCAGACAG AGCCCCTCAG ACCGTGAAGA GTACGTGAAG 
CGGAAGCAGC GCTATGAGGT GGACTTCAAC CTCGAACCTT TCGCCGGCCT CACGCCCGAG 
TACATGGAAA TGATCATTCA GTTCGGCTTT GTCACCCTGT TTGTTGCGTC CTTCCCTCTG 
GCTCCACTCT TCGCCCTGCT AAACAACATC ATTGAGATCC GCCTGGATGC CAAAAAGTTT 
GTCACCGAGC TACGGAGGCC AGTAGCCATC AGAGCCAAAG ACATCGGCAT CTGGTATAAC 
ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC ATCATTAATG CCTTTGTGAT CTCCTTCACG 
TCTGACTTCA TCCCTCGCCT GGTGTACCTC TACATGTACA GTCAGAATGG GACCATGCAC 
GGCTTCGTCA ATCACACGCT CTCTTCCTTC AATGTCAGCG ACTTCCAGAA TGGCACAGCA 
CCCAATGACC CACTGGACCT GGGCTATGAG GTTCAGATCT GCAGGTATAA AGATTACCGG 
GAACCCCCAT GGTCAGAACA CAAGTATGAC ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC 
GCCCGACTGG CATTTGTCAT TGTCTTCCAG AACCTGGTGA TGTTCATGAG TGACTTTGTG 
GACTGGGTGA TCCCTGATAT CCCCAAAGAC ATCAGCCAGC AGATCCACAA AGAGAAGGTT 
CTCATGGTGG AGCTGTTTAT GCGTGAGGAG CAGGGCAAGC AGCAGCTACT GGACACATGG 
ATGGAGAAGG AGAAGCCAAG GGATGTGCCT TGTAACAACC ACAGCCCCAC AACCCACCCA 
GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240277.1
CDS	179..3115
Translation	MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_011241975.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241975.2

ORF Insert Sequence:

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA CCGACAAGGT GAAGCTGACC 
TGGAGGGACC GATTCCCAGC CTATTTCACC AATCTTGTCT CCATCATCTT CATGATCGCA 
GTGACATTTG CAATCGTCCT CGGAGTTATC ATCTATAGAA TCTCCACAGC TGCAGCCTTG 
GCCATGAACT CCTCCCCGTC TGTGCGGTCC AACATCCGGG TTACAGTCAC GGCCACCGCT 
GTTATCATCA ACCTCGTGGT CATCATTCTG CTGGATGAAG TTTACGGCTG CATTGCCAGG 
TGGCTCACCA AGATTGAGGT CCCAAAGACA GAGAAGAGCT TTGAGGAGAG GCTAACCTTC 
AAGGCCTTCC TGCTCAAGTT TGTGAACTCT TACACTCCCA TCTTCTATGT CGCCTTCTTC 
AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC TACGTGTACA TCTTCCGCTC TTTCCGGATG 
GAGGAGTGTG CCCCGGGCGG CTGCCTCATG GAGCTCTGTA TCCAGCTGAG CATCATTATG 
CTGGGCAAGC AGCTAATCCA GAACAATCTC TTCGAGATTG GCATCCCGAA GATGAAAAAG 
TTCATCCGCT ACCTGAAGCT GCGCAGACAG AGCCCCTCAG ACCGTGAAGA GTACGTGAAG 
CGGAAGCAGC GCTATGAGGT GGACTTCAAC CTCGAACCTT TCGCCGGCCT CACGCCCGAG 
TACATGGAAA TGATCATTCA GTTCGGCTTT GTCACCCTGT TTGTTGCGTC CTTCCCTCTG 
GCTCCACTCT TCGCCCTGCT AAACAACATC ATTGAGATCC GCCTGGATGC CAAAAAGTTT 
GTCACCGAGC TACGGAGGCC AGTAGCCATC AGAGCCAAAG ACATCGGCAT CTGGTATAAC 
ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC ATCATTAATG CCTTTGTGAT CTCCTTCACG 
TCTGACTTCA TCCCTCGCCT GGTGTACCTC TACATGTACA GTCAGAATGG GACCATGCAC 
GGCTTCGTCA ATCACACGCT CTCTTCCTTC AATGTCAGCG ACTTCCAGAA TGGCACAGCA 
CCCAATGACC CACTGGACCT GGGCTATGAG GTTCAGATCT GCAGGTATAA AGATTACCGG 
GAACCCCCAT GGTCAGAACA CAAGTATGAC ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC 
GCCCGACTGG CATTTGTCAT TGTCTTCCAG AACCTGGTGA TGTTCATGAG TGACTTTGTG 
GACTGGGTGA TCCCTGATAT CCCCAAAGAC ATCAGCCAGC AGATCCACAA AGAGAAGGTT 
CTCATGGTGG AGCTGTTTAT GCGTGAGGAG CAGGGCAAGC AGCAGCTACT GGACACATGG 
ATGGAGAAGG AGAAGCCAAG GGATGTGCCT TGTAACAACC ACAGCCCCAC AACCCACCCA 
GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_011241976.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240278.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X17, mRNA.

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CloneID	OMu72038
Clone ID Related Accession (Same CDS sequence)	XM_011241976.2
Accession Version	XM_011241976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240278.1
CDS	580..2520
Translation	MLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_011241976.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241976.2

ORF Insert Sequence:

ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( NM_178642.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848757.4 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 1, mRNA.

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CloneID	OMu22943
Clone ID Related Accession (Same CDS sequence)	NM_178642.5
Accession Version	NM_178642.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1552665600000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB569237.1, AK052589.1 and BE953630.1. On May 28, 2011 this sequence version replaced NM_178642.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052589.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GTATCACCAG CCTCCTGGCC AATGGCGTAT ACTCAGCTGC ATACCCTCTG 
CACGATGGGG ACTATGAGGG TGACAACGTT GAGTTCAACG ACAGGAAACT CCTGTATGAG 
GAATGGGCAA GTTACGGAGT CTTCTACAAA TACCAGCCCA TTGACCTGGT CAGGAAATAC 
TTTGGTGAGA AGGTTGGCCT GTACTTTGCC TGGCTTGGAG CCTACACCCA GATGCTCATC 
CCTGCCTCGA TCGTGGGTGT CATTGTCTTT CTCTATGGAT GTGCCACTGT GGACGAAAAC 
ATCCCCAGTA TGGAGATGTG TGACCAGAGA TACAACATCA CCATGTGTCC TCTGTGTGAC 
AAGACCTGCA GCTACTGGAA GATGAGCTCA GCCTGTGCCA CAGCCCGTGC CAGTCACCTT 
TTTGATAACC CTGCCACCGT CTTCTTCTCT GTGTTTATGG CCCTCTGGGC TGCCACTTTC 
ATGGAGCACT GGAAACGGAA GCAGATGAGG CTCAACTACC GATGGGACCT CACAGGCTTC 
GAGGAGGAGG AGGAAGCTGT CAAGGATCAT CCCAGAGCAG AGTATGAAGC CAGAGTCTTA 
GAGAAGTCAC TGAGAAAAGA ATCCAGAAAC AAAGAGACCG ACAAGGTGAA GCTGACCTGG 
AGGGACCGAT TCCCAGCCTA TTTCACCAAT CTTGTCTCCA TCATCTTCAT GATCGCAGTG 
ACATTTGCAA TCGTCCTCGG AGTTATCATC TATAGAATCT CCACAGCTGC AGCCTTGGCC 
ATGAACTCCT CCCCGTCTGT GCGGTCCAAC ATCCGGGTTA CAGTCACGGC CACCGCTGTT 
ATCATCAACC TCGTGGTCAT CATTCTGCTG GATGAAGTTT ACGGCTGCAT TGCCAGGTGG 
CTCACCAAGA TTGAGGTCCC AAAGACAGAG AAGAGCTTTG AGGAGAGGCT AACCTTCAAG 
GCCTTCCTGC TCAAGTTTGT GAACTCTTAC ACTCCCATCT TCTATGTCGC CTTCTTCAAA 
GGCCGGTTTG TTGGTCGGCC CGGTGACTAC GTGTACATCT TCCGCTCTTT CCGGATGGAG 
GAGTGTGCCC CGGGCGGCTG CCTCATGGAG CTCTGTATCC AGCTGAGCAT CATTATGCTG 
GGCAAGCAGC TAATCCAGAA CAATCTCTTC GAGATTGGCA TCCCGAAGAT GAAAAAGTTC 
ATCCGCTACC TGAAGCTGCG CAGACAGAGC CCCTCAGACC GTGAAGAGTA CGTGAAGCGG 
AAGCAGCGCT ATGAGGTGGA CTTCAACCTC GAACCTTTCG CCGGCCTCAC GCCCGAGTAC 
ATGGAAATGA TCATTCAGTT CGGCTTTGTC ACCCTGTTTG TTGCGTCCTT CCCTCTGGCT 
CCACTCTTCG CCCTGCTAAA CAACATCATT GAGATCCGCC TGGATGCCAA AAAGTTTGTC 
ACCGAGCTAC GGAGGCCAGT AGCCATCAGA GCCAAAGACA TCGGCATCTG GTATAACATC 
CTCAGAGGTG TTGGGAAGCT GGCTGTCATC ATTAATGCCT TTGTGATCTC CTTCACGTCT 
GACTTCATCC CTCGCCTGGT GTACCTCTAC ATGTACAGTC AGAATGGGAC CATGCACGGC 
TTCGTCAATC ACACGCTCTC TTCCTTCAAT GTCAGCGACT TCCAGAATGG CACAGCACCC 
AATGACCCAC TGGACCTGGG CTATGAGGTT CAGATCTGCA GGTATAAAGA TTACCGGGAA 
CCCCCATGGT CAGAACACAA GTATGACATC TCCAAAGACT TCTGGGCTGT CCTGGCCGCC 
CGACTGGCAT TTGTCATTGT CTTCCAGAAC CTGGTGATGT TCATGAGTGA CTTTGTGGAC 
TGGGTGATCC CTGATATCCC CAAAGACATC AGCCAGCAGA TCCACAAAGA GAAGGTTCTC 
ATGGTGGAGC TGTTTATGCG TGAGGAGCAG GGCAAGCAGC AGCTACTGGA CACATGGATG 
GAGAAGGAGA AGCCAAGGGA TGTGCCTTGT AACAACCACA GCCCCACAAC CCACCCAGAG 
GCAGGCGACG GCAGCCCAGT CCCCAGCTAC GAGTACCATG GGGACGCGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848757.4
CDS	98..3151
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	NM_178642.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178642.5

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GTATCACCAG CCTCCTGGCC AATGGCGTAT ACTCAGCTGC ATACCCTCTG 
CACGATGGGG ACTATGAGGG TGACAACGTT GAGTTCAACG ACAGGAAACT CCTGTATGAG 
GAATGGGCAA GTTACGGAGT CTTCTACAAA TACCAGCCCA TTGACCTGGT CAGGAAATAC 
TTTGGTGAGA AGGTTGGCCT GTACTTTGCC TGGCTTGGAG CCTACACCCA GATGCTCATC 
CCTGCCTCGA TCGTGGGTGT CATTGTCTTT CTCTATGGAT GTGCCACTGT GGACGAAAAC 
ATCCCCAGTA TGGAGATGTG TGACCAGAGA TACAACATCA CCATGTGTCC TCTGTGTGAC 
AAGACCTGCA GCTACTGGAA GATGAGCTCA GCCTGTGCCA CAGCCCGTGC CAGTCACCTT 
TTTGATAACC CTGCCACCGT CTTCTTCTCT GTGTTTATGG CCCTCTGGGC TGCCACTTTC 
ATGGAGCACT GGAAACGGAA GCAGATGAGG CTCAACTACC GATGGGACCT CACAGGCTTC 
GAGGAGGAGG AGGAAGCTGT CAAGGATCAT CCCAGAGCAG AGTATGAAGC CAGAGTCTTA 
GAGAAGTCAC TGAGAAAAGA ATCCAGAAAC AAAGAGACCG ACAAGGTGAA GCTGACCTGG 
AGGGACCGAT TCCCAGCCTA TTTCACCAAT CTTGTCTCCA TCATCTTCAT GATCGCAGTG 
ACATTTGCAA TCGTCCTCGG AGTTATCATC TATAGAATCT CCACAGCTGC AGCCTTGGCC 
ATGAACTCCT CCCCGTCTGT GCGGTCCAAC ATCCGGGTTA CAGTCACGGC CACCGCTGTT 
ATCATCAACC TCGTGGTCAT CATTCTGCTG GATGAAGTTT ACGGCTGCAT TGCCAGGTGG 
CTCACCAAGA TTGAGGTCCC AAAGACAGAG AAGAGCTTTG AGGAGAGGCT AACCTTCAAG 
GCCTTCCTGC TCAAGTTTGT GAACTCTTAC ACTCCCATCT TCTATGTCGC CTTCTTCAAA 
GGCCGGTTTG TTGGTCGGCC CGGTGACTAC GTGTACATCT TCCGCTCTTT CCGGATGGAG 
GAGTGTGCCC CGGGCGGCTG CCTCATGGAG CTCTGTATCC AGCTGAGCAT CATTATGCTG 
GGCAAGCAGC TAATCCAGAA CAATCTCTTC GAGATTGGCA TCCCGAAGAT GAAAAAGTTC 
ATCCGCTACC TGAAGCTGCG CAGACAGAGC CCCTCAGACC GTGAAGAGTA CGTGAAGCGG 
AAGCAGCGCT ATGAGGTGGA CTTCAACCTC GAACCTTTCG CCGGCCTCAC GCCCGAGTAC 
ATGGAAATGA TCATTCAGTT CGGCTTTGTC ACCCTGTTTG TTGCGTCCTT CCCTCTGGCT 
CCACTCTTCG CCCTGCTAAA CAACATCATT GAGATCCGCC TGGATGCCAA AAAGTTTGTC 
ACCGAGCTAC GGAGGCCAGT AGCCATCAGA GCCAAAGACA TCGGCATCTG GTATAACATC 
CTCAGAGGTG TTGGGAAGCT GGCTGTCATC ATTAATGCCT TTGTGATCTC CTTCACGTCT 
GACTTCATCC CTCGCCTGGT GTACCTCTAC ATGTACAGTC AGAATGGGAC CATGCACGGC 
TTCGTCAATC ACACGCTCTC TTCCTTCAAT GTCAGCGACT TCCAGAATGG CACAGCACCC 
AATGACCCAC TGGACCTGGG CTATGAGGTT CAGATCTGCA GGTATAAAGA TTACCGGGAA 
CCCCCATGGT CAGAACACAA GTATGACATC TCCAAAGACT TCTGGGCTGT CCTGGCCGCC 
CGACTGGCAT TTGTCATTGT CTTCCAGAAC CTGGTGATGT TCATGAGTGA CTTTGTGGAC 
TGGGTGATCC CTGATATCCC CAAAGACATC AGCCAGCAGA TCCACAAAGA GAAGGTTCTC 
ATGGTGGAGC TGTTTATGCG TGAGGAGCAG GGCAAGCAGC AGCTACTGGA CACATGGATG 
GAGAAGGAGA AGCCAAGGGA TGTGCCTTGT AACAACCACA GCCCCACAAC CCACCCAGAG 
GCAGGCGACG GCAGCCCAGT CCCCAGCTAC GAGTACCATG GGGACGCGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( NM_001242349.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001229278.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23048
Clone ID Related Accession (Same CDS sequence)	NM_001242349.1
Accession Version	NM_001242349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1552665600000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB569237.1, CF741645.1, BC062959.1 and BE953630.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and lacks an internal coding exon, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062959.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TATACTCAGC TGCATACCCT 
CTGCACGATG GGGACTATGA GGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGTTACGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGTG AGAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CGATCGTGGG TGTCATTGTC TTTCTCTATG GATGTGCCAC TGTGGACGAA 
AACATCCCCA GTATGGAGAT GTGTGACCAG AGATACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCCCG TGCCAGTCAC 
CTTTTTGATA ACCCTGCCAC CGTCTTCTTC TCTGTGTTTA TGGCCCTCTG GGCTGCCACT 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGGCTCAACT ACCGATGGGA CCTCACAGGC 
TTCGAGGAGG AGGAGGATCA TCCCAGAGCA GAGTATGAAG CCAGAGTCTT AGAGAAGTCA 
CTGAGAAAAG AATCCAGAAA CAAAGAGACC GACAAGGTGA AGCTGACCTG GAGGGACCGA 
TTCCCAGCCT ATTTCACCAA TCTTGTCTCC ATCATCTTCA TGATCGCAGT GACATTTGCA 
ATCGTCCTCG GAGTTATCAT CTATAGAATC TCCACAGCTG CAGCCTTGGC CATGAACTCC 
TCCCCGTCTG TGCGGTCCAA CATCCGGGTT ACAGTCACGG CCACCGCTGT TATCATCAAC 
CTCGTGGTCA TCATTCTGCT GGATGAAGTT TACGGCTGCA TTGCCAGGTG GCTCACCAAG 
ATTGAGGTCC CAAAGACAGA GAAGAGCTTT GAGGAGAGGC TAACCTTCAA GGCCTTCCTG 
CTCAAGTTTG TGAACTCTTA CACTCCCATC TTCTATGTCG CCTTCTTCAA AGGCCGGTTT 
GTTGGTCGGC CCGGTGACTA CGTGTACATC TTCCGCTCTT TCCGGATGGA GGAGTGTGCC 
CCGGGCGGCT GCCTCATGGA GCTCTGTATC CAGCTGAGCA TCATTATGCT GGGCAAGCAG 
CTAATCCAGA ACAATCTCTT CGAGATTGGC ATCCCGAAGA TGAAAAAGTT CATCCGCTAC 
CTGAAGCTGC GCAGACAGAG CCCCTCAGAC CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC 
TATGAGGTGG ACTTCAACCT CGAACCTTTC GCCGGCCTCA CGCCCGAGTA CATGGAAATG 
ATCATTCAGT TCGGCTTTGT CACCCTGTTT GTTGCGTCCT TCCCTCTGGC TCCACTCTTC 
GCCCTGCTAA ACAACATCAT TGAGATCCGC CTGGATGCCA AAAAGTTTGT CACCGAGCTA 
CGGAGGCCAG TAGCCATCAG AGCCAAAGAC ATCGGCATCT GGTATAACAT CCTCAGAGGT 
GTTGGGAAGC TGGCTGTCAT CATTAATGCC TTTGTGATCT CCTTCACGTC TGACTTCATC 
CCTCGCCTGG TGTACCTCTA CATGTACAGT CAGAATGGGA CCATGCACGG CTTCGTCAAT 
CACACGCTCT CTTCCTTCAA TGTCAGCGAC TTCCAGAATG GCACAGCACC CAATGACCCA 
CTGGACCTGG GCTATGAGGT TCAGATCTGC AGGTATAAAG ATTACCGGGA ACCCCCATGG 
TCAGAACACA AGTATGACAT CTCCAAAGAC TTCTGGGCTG TCCTGGCCGC CCGACTGGCA 
TTTGTCATTG TCTTCCAGAA CCTGGTGATG TTCATGAGTG ACTTTGTGGA CTGGGTGATC 
CCTGATATCC CCAAAGACAT CAGCCAGCAG ATCCACAAAG AGAAGGTTCT CATGGTGGAG 
CTGTTTATGC GTGAGGAGCA GGGCAAGCAG CAGCTACTGG ACACATGGAT GGAGAAGGAG 
AAGCCAAGGG ATGTGCCTTG TAACAACCAC AGCCCCACAA CCCACCCAGA GGCAGGCGAC 
GGCAGCCCAG TCCCCAGCTA CGAGTACCAT GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229278.1
CDS	98..3142
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	NM_001242349.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242349.1

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TATACTCAGC TGCATACCCT 
CTGCACGATG GGGACTATGA GGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGTTACGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGTG AGAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CGATCGTGGG TGTCATTGTC TTTCTCTATG GATGTGCCAC TGTGGACGAA 
AACATCCCCA GTATGGAGAT GTGTGACCAG AGATACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCCCG TGCCAGTCAC 
CTTTTTGATA ACCCTGCCAC CGTCTTCTTC TCTGTGTTTA TGGCCCTCTG GGCTGCCACT 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGGCTCAACT ACCGATGGGA CCTCACAGGC 
TTCGAGGAGG AGGAGGATCA TCCCAGAGCA GAGTATGAAG CCAGAGTCTT AGAGAAGTCA 
CTGAGAAAAG AATCCAGAAA CAAAGAGACC GACAAGGTGA AGCTGACCTG GAGGGACCGA 
TTCCCAGCCT ATTTCACCAA TCTTGTCTCC ATCATCTTCA TGATCGCAGT GACATTTGCA 
ATCGTCCTCG GAGTTATCAT CTATAGAATC TCCACAGCTG CAGCCTTGGC CATGAACTCC 
TCCCCGTCTG TGCGGTCCAA CATCCGGGTT ACAGTCACGG CCACCGCTGT TATCATCAAC 
CTCGTGGTCA TCATTCTGCT GGATGAAGTT TACGGCTGCA TTGCCAGGTG GCTCACCAAG 
ATTGAGGTCC CAAAGACAGA GAAGAGCTTT GAGGAGAGGC TAACCTTCAA GGCCTTCCTG 
CTCAAGTTTG TGAACTCTTA CACTCCCATC TTCTATGTCG CCTTCTTCAA AGGCCGGTTT 
GTTGGTCGGC CCGGTGACTA CGTGTACATC TTCCGCTCTT TCCGGATGGA GGAGTGTGCC 
CCGGGCGGCT GCCTCATGGA GCTCTGTATC CAGCTGAGCA TCATTATGCT GGGCAAGCAG 
CTAATCCAGA ACAATCTCTT CGAGATTGGC ATCCCGAAGA TGAAAAAGTT CATCCGCTAC 
CTGAAGCTGC GCAGACAGAG CCCCTCAGAC CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC 
TATGAGGTGG ACTTCAACCT CGAACCTTTC GCCGGCCTCA CGCCCGAGTA CATGGAAATG 
ATCATTCAGT TCGGCTTTGT CACCCTGTTT GTTGCGTCCT TCCCTCTGGC TCCACTCTTC 
GCCCTGCTAA ACAACATCAT TGAGATCCGC CTGGATGCCA AAAAGTTTGT CACCGAGCTA 
CGGAGGCCAG TAGCCATCAG AGCCAAAGAC ATCGGCATCT GGTATAACAT CCTCAGAGGT 
GTTGGGAAGC TGGCTGTCAT CATTAATGCC TTTGTGATCT CCTTCACGTC TGACTTCATC 
CCTCGCCTGG TGTACCTCTA CATGTACAGT CAGAATGGGA CCATGCACGG CTTCGTCAAT 
CACACGCTCT CTTCCTTCAA TGTCAGCGAC TTCCAGAATG GCACAGCACC CAATGACCCA 
CTGGACCTGG GCTATGAGGT TCAGATCTGC AGGTATAAAG ATTACCGGGA ACCCCCATGG 
TCAGAACACA AGTATGACAT CTCCAAAGAC TTCTGGGCTG TCCTGGCCGC CCGACTGGCA 
TTTGTCATTG TCTTCCAGAA CCTGGTGATG TTCATGAGTG ACTTTGTGGA CTGGGTGATC 
CCTGATATCC CCAAAGACAT CAGCCAGCAG ATCCACAAAG AGAAGGTTCT CATGGTGGAG 
CTGTTTATGC GTGAGGAGCA GGGCAAGCAG CAGCTACTGG ACACATGGAT GGAGAAGGAG 
AAGCCAAGGG ATGTGCCTTG TAACAACCAC AGCCCCACAA CCCACCCAGA GGCAGGCGAC 
GGCAGCCCAG TCCCCAGCTA CGAGTACCAT GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508458.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508521.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X6, mRNA.

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CloneID	OMu40133
Clone ID Related Accession (Same CDS sequence)	XM_006508458.4 , XM_006508458.3
Accession Version	XM_006508458.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508458.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508521.1
CDS	144..3344
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508458.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508458.4

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508458.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508521.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu40133
Clone ID Related Accession (Same CDS sequence)	XM_006508458.4 , XM_006508458.3
Accession Version	XM_006508458.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508458.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508521.1
CDS	148..3348
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508458.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508458.3

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508459.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508522.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu40134
Clone ID Related Accession (Same CDS sequence)	XM_006508459.4 , XM_006508459.3
Accession Version	XM_006508459.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508459.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTATACTC AGCTGCATAC 
CCTCTGCACG ATGGGGACTA TGAGGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGTTA CGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GTGAGAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCGATCGT GGGTGTCATT GTCTTTCTCT ATGGATGTGC CACTGTGGAC 
GAAAACATCC CCAGTATGGA GATGTGTGAC CAGAGATACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC CCGTGCCAGT 
CACCTTTTTG ATAACCCTGC CACCGTCTTC TTCTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACTTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGGCTCA ACTACCGATG GGACCTCACA 
GGCTTCGAGG AGGAGGAGGA AGCTGTCAAG GATCATCCCA GAGCAGAGTA TGAAGCCAGA 
GTCTTAGAGA AGTCACTGAG AAAAGAATCC AGAAACAAAG AGAAGTGCCG AAATGTTCCA 
GAAGAACCGA CCAACAAATG GAAGCAGAGG GTTAGGACAG CCATGGCAGG GGTGAAATTG 
ACCGACAAGG TGAAGCTGAC CTGGAGGGAC CGATTCCCAG CCTATTTCAC CAATCTTGTC 
TCCATCATCT TCATGATCGC AGTGACATTT GCAATCGTCC TCGGAGTTAT CATCTATAGA 
ATCTCCACAG CTGCAGCCTT GGCCATGAAC TCCTCCCCGT CTGTGCGGTC CAACATCCGG 
GTTACAGTCA CGGCCACCGC TGTTATCATC AACCTCGTGG TCATCATTCT GCTGGATGAA 
GTTTACGGCT GCATTGCCAG GTGGCTCACC AAGATTGAGG TCCCAAAGAC AGAGAAGAGC 
TTTGAGGAGA GGCTAACCTT CAAGGCCTTC CTGCTCAAGT TTGTGAACTC TTACACTCCC 
ATCTTCTATG TCGCCTTCTT CAAAGGCCGG TTTGTTGGTC GGCCCGGTGA CTACGTGTAC 
ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT GCCCCGGGCG GCTGCCTCAT GGAGCTCTGT 
ATCCAGCTGA GCATCATTAT GCTGGGCAAG CAGCTAATCC AGAACAATCT CTTCGAGATT 
GGCATCCCGA AGATGAAAAA GTTCATCCGC TACCTGAAGC TGCGCAGACA GAGCCCCTCA 
GACCGTGAAG AGTACGTGAA GCGGAAGCAG CGCTATGAGG TGGACTTCAA CCTCGAACCT 
TTCGCCGGCC TCACGCCCGA GTACATGGAA ATGATCATTC AGTTCGGCTT TGTCACCCTG 
TTTGTTGCGT CCTTCCCTCT GGCTCCACTC TTCGCCCTGC TAAACAACAT CATTGAGATC 
CGCCTGGATG CCAAAAAGTT TGTCACCGAG CTACGGAGGC CAGTAGCCAT CAGAGCCAAA 
GACATCGGCA TCTGGTATAA CATCCTCAGA GGTGTTGGGA AGCTGGCTGT CATCATTAAT 
GCCTTTGTGA TCTCCTTCAC GTCTGACTTC ATCCCTCGCC TGGTGTACCT CTACATGTAC 
AGTCAGAATG GGACCATGCA CGGCTTCGTC AATCACACGC TCTCTTCCTT CAATGTCAGC 
GACTTCCAGA ATGGCACAGC ACCCAATGAC CCACTGGACC TGGGCTATGA GGTTCAGATC 
TGCAGGTATA AAGATTACCG GGAACCCCCA TGGTCAGAAC ACAAGTATGA CATCTCCAAA 
GACTTCTGGG CTGTCCTGGC CGCCCGACTG GCATTTGTCA TTGTCTTCCA GAACCTGGTG 
ATGTTCATGA GTGACTTTGT GGACTGGGTG ATCCCTGATA TCCCCAAAGA CATCAGCCAG 
CAGATCCACA AAGAGAAGGT TCTCATGGTG GAGCTGTTTA TGCGTGAGGA GCAGGGCAAG 
CAGCAGCTAC TGGACACATG GATGGAGAAG GAGAAGCCAA GGGATGTGCC TTGTAACAAC 
CACAGCCCCA CAACCCACCC AGAGGCAGGC GACGGCAGCC CAGTCCCCAG CTACGAGTAC 
CATGGGGACG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508522.1
CDS	144..3341
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508459.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508459.4

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTATACTC AGCTGCATAC 
CCTCTGCACG ATGGGGACTA TGAGGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGTTA CGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GTGAGAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCGATCGT GGGTGTCATT GTCTTTCTCT ATGGATGTGC CACTGTGGAC 
GAAAACATCC CCAGTATGGA GATGTGTGAC CAGAGATACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC CCGTGCCAGT 
CACCTTTTTG ATAACCCTGC CACCGTCTTC TTCTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACTTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGGCTCA ACTACCGATG GGACCTCACA 
GGCTTCGAGG AGGAGGAGGA AGCTGTCAAG GATCATCCCA GAGCAGAGTA TGAAGCCAGA 
GTCTTAGAGA AGTCACTGAG AAAAGAATCC AGAAACAAAG AGAAGTGCCG AAATGTTCCA 
GAAGAACCGA CCAACAAATG GAAGCAGAGG GTTAGGACAG CCATGGCAGG GGTGAAATTG 
ACCGACAAGG TGAAGCTGAC CTGGAGGGAC CGATTCCCAG CCTATTTCAC CAATCTTGTC 
TCCATCATCT TCATGATCGC AGTGACATTT GCAATCGTCC TCGGAGTTAT CATCTATAGA 
ATCTCCACAG CTGCAGCCTT GGCCATGAAC TCCTCCCCGT CTGTGCGGTC CAACATCCGG 
GTTACAGTCA CGGCCACCGC TGTTATCATC AACCTCGTGG TCATCATTCT GCTGGATGAA 
GTTTACGGCT GCATTGCCAG GTGGCTCACC AAGATTGAGG TCCCAAAGAC AGAGAAGAGC 
TTTGAGGAGA GGCTAACCTT CAAGGCCTTC CTGCTCAAGT TTGTGAACTC TTACACTCCC 
ATCTTCTATG TCGCCTTCTT CAAAGGCCGG TTTGTTGGTC GGCCCGGTGA CTACGTGTAC 
ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT GCCCCGGGCG GCTGCCTCAT GGAGCTCTGT 
ATCCAGCTGA GCATCATTAT GCTGGGCAAG CAGCTAATCC AGAACAATCT CTTCGAGATT 
GGCATCCCGA AGATGAAAAA GTTCATCCGC TACCTGAAGC TGCGCAGACA GAGCCCCTCA 
GACCGTGAAG AGTACGTGAA GCGGAAGCAG CGCTATGAGG TGGACTTCAA CCTCGAACCT 
TTCGCCGGCC TCACGCCCGA GTACATGGAA ATGATCATTC AGTTCGGCTT TGTCACCCTG 
TTTGTTGCGT CCTTCCCTCT GGCTCCACTC TTCGCCCTGC TAAACAACAT CATTGAGATC 
CGCCTGGATG CCAAAAAGTT TGTCACCGAG CTACGGAGGC CAGTAGCCAT CAGAGCCAAA 
GACATCGGCA TCTGGTATAA CATCCTCAGA GGTGTTGGGA AGCTGGCTGT CATCATTAAT 
GCCTTTGTGA TCTCCTTCAC GTCTGACTTC ATCCCTCGCC TGGTGTACCT CTACATGTAC 
AGTCAGAATG GGACCATGCA CGGCTTCGTC AATCACACGC TCTCTTCCTT CAATGTCAGC 
GACTTCCAGA ATGGCACAGC ACCCAATGAC CCACTGGACC TGGGCTATGA GGTTCAGATC 
TGCAGGTATA AAGATTACCG GGAACCCCCA TGGTCAGAAC ACAAGTATGA CATCTCCAAA 
GACTTCTGGG CTGTCCTGGC CGCCCGACTG GCATTTGTCA TTGTCTTCCA GAACCTGGTG 
ATGTTCATGA GTGACTTTGT GGACTGGGTG ATCCCTGATA TCCCCAAAGA CATCAGCCAG 
CAGATCCACA AAGAGAAGGT TCTCATGGTG GAGCTGTTTA TGCGTGAGGA GCAGGGCAAG 
CAGCAGCTAC TGGACACATG GATGGAGAAG GAGAAGCCAA GGGATGTGCC TTGTAACAAC 
CACAGCCCCA CAACCCACCC AGAGGCAGGC GACGGCAGCC CAGTCCCCAG CTACGAGTAC 
CATGGGGACG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508459.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508522.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu40134
Clone ID Related Accession (Same CDS sequence)	XM_006508459.4 , XM_006508459.3
Accession Version	XM_006508459.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTATACTC AGCTGCATAC 
CCTCTGCACG ATGGGGACTA TGAGGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGTTA CGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GTGAGAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCGATCGT GGGTGTCATT GTCTTTCTCT ATGGATGTGC CACTGTGGAC 
GAAAACATCC CCAGTATGGA GATGTGTGAC CAGAGATACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC CCGTGCCAGT 
CACCTTTTTG ATAACCCTGC CACCGTCTTC TTCTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACTTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGGCTCA ACTACCGATG GGACCTCACA 
GGCTTCGAGG AGGAGGAGGA AGCTGTCAAG GATCATCCCA GAGCAGAGTA TGAAGCCAGA 
GTCTTAGAGA AGTCACTGAG AAAAGAATCC AGAAACAAAG AGAAGTGCCG AAATGTTCCA 
GAAGAACCGA CCAACAAATG GAAGCAGAGG GTTAGGACAG CCATGGCAGG GGTGAAATTG 
ACCGACAAGG TGAAGCTGAC CTGGAGGGAC CGATTCCCAG CCTATTTCAC CAATCTTGTC 
TCCATCATCT TCATGATCGC AGTGACATTT GCAATCGTCC TCGGAGTTAT CATCTATAGA 
ATCTCCACAG CTGCAGCCTT GGCCATGAAC TCCTCCCCGT CTGTGCGGTC CAACATCCGG 
GTTACAGTCA CGGCCACCGC TGTTATCATC AACCTCGTGG TCATCATTCT GCTGGATGAA 
GTTTACGGCT GCATTGCCAG GTGGCTCACC AAGATTGAGG TCCCAAAGAC AGAGAAGAGC 
TTTGAGGAGA GGCTAACCTT CAAGGCCTTC CTGCTCAAGT TTGTGAACTC TTACACTCCC 
ATCTTCTATG TCGCCTTCTT CAAAGGCCGG TTTGTTGGTC GGCCCGGTGA CTACGTGTAC 
ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT GCCCCGGGCG GCTGCCTCAT GGAGCTCTGT 
ATCCAGCTGA GCATCATTAT GCTGGGCAAG CAGCTAATCC AGAACAATCT CTTCGAGATT 
GGCATCCCGA AGATGAAAAA GTTCATCCGC TACCTGAAGC TGCGCAGACA GAGCCCCTCA 
GACCGTGAAG AGTACGTGAA GCGGAAGCAG CGCTATGAGG TGGACTTCAA CCTCGAACCT 
TTCGCCGGCC TCACGCCCGA GTACATGGAA ATGATCATTC AGTTCGGCTT TGTCACCCTG 
TTTGTTGCGT CCTTCCCTCT GGCTCCACTC TTCGCCCTGC TAAACAACAT CATTGAGATC 
CGCCTGGATG CCAAAAAGTT TGTCACCGAG CTACGGAGGC CAGTAGCCAT CAGAGCCAAA 
GACATCGGCA TCTGGTATAA CATCCTCAGA GGTGTTGGGA AGCTGGCTGT CATCATTAAT 
GCCTTTGTGA TCTCCTTCAC GTCTGACTTC ATCCCTCGCC TGGTGTACCT CTACATGTAC 
AGTCAGAATG GGACCATGCA CGGCTTCGTC AATCACACGC TCTCTTCCTT CAATGTCAGC 
GACTTCCAGA ATGGCACAGC ACCCAATGAC CCACTGGACC TGGGCTATGA GGTTCAGATC 
TGCAGGTATA AAGATTACCG GGAACCCCCA TGGTCAGAAC ACAAGTATGA CATCTCCAAA 
GACTTCTGGG CTGTCCTGGC CGCCCGACTG GCATTTGTCA TTGTCTTCCA GAACCTGGTG 
ATGTTCATGA GTGACTTTGT GGACTGGGTG ATCCCTGATA TCCCCAAAGA CATCAGCCAG 
CAGATCCACA AAGAGAAGGT TCTCATGGTG GAGCTGTTTA TGCGTGAGGA GCAGGGCAAG 
CAGCAGCTAC TGGACACATG GATGGAGAAG GAGAAGCCAA GGGATGTGCC TTGTAACAAC 
CACAGCCCCA CAACCCACCC AGAGGCAGGC GACGGCAGCC CAGTCCCCAG CTACGAGTAC 
CATGGGGACG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508522.1
CDS	147..3344
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508459.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508459.3

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTATACTC AGCTGCATAC 
CCTCTGCACG ATGGGGACTA TGAGGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGTTA CGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GTGAGAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCGATCGT GGGTGTCATT GTCTTTCTCT ATGGATGTGC CACTGTGGAC 
GAAAACATCC CCAGTATGGA GATGTGTGAC CAGAGATACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC CCGTGCCAGT 
CACCTTTTTG ATAACCCTGC CACCGTCTTC TTCTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACTTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGGCTCA ACTACCGATG GGACCTCACA 
GGCTTCGAGG AGGAGGAGGA AGCTGTCAAG GATCATCCCA GAGCAGAGTA TGAAGCCAGA 
GTCTTAGAGA AGTCACTGAG AAAAGAATCC AGAAACAAAG AGAAGTGCCG AAATGTTCCA 
GAAGAACCGA CCAACAAATG GAAGCAGAGG GTTAGGACAG CCATGGCAGG GGTGAAATTG 
ACCGACAAGG TGAAGCTGAC CTGGAGGGAC CGATTCCCAG CCTATTTCAC CAATCTTGTC 
TCCATCATCT TCATGATCGC AGTGACATTT GCAATCGTCC TCGGAGTTAT CATCTATAGA 
ATCTCCACAG CTGCAGCCTT GGCCATGAAC TCCTCCCCGT CTGTGCGGTC CAACATCCGG 
GTTACAGTCA CGGCCACCGC TGTTATCATC AACCTCGTGG TCATCATTCT GCTGGATGAA 
GTTTACGGCT GCATTGCCAG GTGGCTCACC AAGATTGAGG TCCCAAAGAC AGAGAAGAGC 
TTTGAGGAGA GGCTAACCTT CAAGGCCTTC CTGCTCAAGT TTGTGAACTC TTACACTCCC 
ATCTTCTATG TCGCCTTCTT CAAAGGCCGG TTTGTTGGTC GGCCCGGTGA CTACGTGTAC 
ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT GCCCCGGGCG GCTGCCTCAT GGAGCTCTGT 
ATCCAGCTGA GCATCATTAT GCTGGGCAAG CAGCTAATCC AGAACAATCT CTTCGAGATT 
GGCATCCCGA AGATGAAAAA GTTCATCCGC TACCTGAAGC TGCGCAGACA GAGCCCCTCA 
GACCGTGAAG AGTACGTGAA GCGGAAGCAG CGCTATGAGG TGGACTTCAA CCTCGAACCT 
TTCGCCGGCC TCACGCCCGA GTACATGGAA ATGATCATTC AGTTCGGCTT TGTCACCCTG 
TTTGTTGCGT CCTTCCCTCT GGCTCCACTC TTCGCCCTGC TAAACAACAT CATTGAGATC 
CGCCTGGATG CCAAAAAGTT TGTCACCGAG CTACGGAGGC CAGTAGCCAT CAGAGCCAAA 
GACATCGGCA TCTGGTATAA CATCCTCAGA GGTGTTGGGA AGCTGGCTGT CATCATTAAT 
GCCTTTGTGA TCTCCTTCAC GTCTGACTTC ATCCCTCGCC TGGTGTACCT CTACATGTAC 
AGTCAGAATG GGACCATGCA CGGCTTCGTC AATCACACGC TCTCTTCCTT CAATGTCAGC 
GACTTCCAGA ATGGCACAGC ACCCAATGAC CCACTGGACC TGGGCTATGA GGTTCAGATC 
TGCAGGTATA AAGATTACCG GGAACCCCCA TGGTCAGAAC ACAAGTATGA CATCTCCAAA 
GACTTCTGGG CTGTCCTGGC CGCCCGACTG GCATTTGTCA TTGTCTTCCA GAACCTGGTG 
ATGTTCATGA GTGACTTTGT GGACTGGGTG ATCCCTGATA TCCCCAAAGA CATCAGCCAG 
CAGATCCACA AAGAGAAGGT TCTCATGGTG GAGCTGTTTA TGCGTGAGGA GCAGGGCAAG 
CAGCAGCTAC TGGACACATG GATGGAGAAG GAGAAGCCAA GGGATGTGCC TTGTAACAAC 
CACAGCCCCA CAACCCACCC AGAGGCAGGC GACGGCAGCC CAGTCCCCAG CTACGAGTAC 
CATGGGGACG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508463.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508526.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMu40138
Clone ID Related Accession (Same CDS sequence)	XM_006508463.4 , XM_006508463.3
Accession Version	XM_006508463.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508463.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC CAGTGAGGGA GCTTCTGCTG ACCAACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGAAGTGCC GAAATGTTCC AGAAGAACCG ACCAACAAAT GGAAGCAGAG GGTTAGGACA 
GCCATGGCAG GGGTGAAATT GACCGACAAG GTGAAGCTGA CCTGGAGGGA CCGATTCCCA 
GCCTATTTCA CCAATCTTGT CTCCATCATC TTCATGATCG CAGTGACATT TGCAATCGTC 
CTCGGAGTTA TCATCTATAG AATCTCCACA GCTGCAGCCT TGGCCATGAA CTCCTCCCCG 
TCTGTGCGGT CCAACATCCG GGTTACAGTC ACGGCCACCG CTGTTATCAT CAACCTCGTG 
GTCATCATTC TGCTGGATGA AGTTTACGGC TGCATTGCCA GGTGGCTCAC CAAGATTGAG 
GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG AGGCTAACCT TCAAGGCCTT CCTGCTCAAG 
TTTGTGAACT CTTACACTCC CATCTTCTAT GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT 
CGGCCCGGTG ACTACGTGTA CATCTTCCGC TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC 
GGCTGCCTCA TGGAGCTCTG TATCCAGCTG AGCATCATTA TGCTGGGCAA GCAGCTAATC 
CAGAACAATC TCTTCGAGAT TGGCATCCCG AAGATGAAAA AGTTCATCCG CTACCTGAAG 
CTGCGCAGAC AGAGCCCCTC AGACCGTGAA GAGTACGTGA AGCGGAAGCA GCGCTATGAG 
GTGGACTTCA ACCTCGAACC TTTCGCCGGC CTCACGCCCG AGTACATGGA AATGATCATT 
CAGTTCGGCT TTGTCACCCT GTTTGTTGCG TCCTTCCCTC TGGCTCCACT CTTCGCCCTG 
CTAAACAACA TCATTGAGAT CCGCCTGGAT GCCAAAAAGT TTGTCACCGA GCTACGGAGG 
CCAGTAGCCA TCAGAGCCAA AGACATCGGC ATCTGGTATA ACATCCTCAG AGGTGTTGGG 
AAGCTGGCTG TCATCATTAA TGCCTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC 
CTGGTGTACC TCTACATGTA CAGTCAGAAT GGGACCATGC ACGGCTTCGT CAATCACACG 
CTCTCTTCCT TCAATGTCAG CGACTTCCAG AATGGCACAG CACCCAATGA CCCACTGGAC 
CTGGGCTATG AGGTTCAGAT CTGCAGGTAT AAAGATTACC GGGAACCCCC ATGGTCAGAA 
CACAAGTATG ACATCTCCAA AGACTTCTGG GCTGTCCTGG CCGCCCGACT GGCATTTGTC 
ATTGTCTTCC AGAACCTGGT GATGTTCATG AGTGACTTTG TGGACTGGGT GATCCCTGAT 
ATCCCCAAAG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TTCTCATGGT GGAGCTGTTT 
ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA CTGGACACAT GGATGGAGAA GGAGAAGCCA 
AGGGATGTGC CTTGTAACAA CCACAGCCCC ACAACCCACC CAGAGGCAGG CGACGGCAGC 
CCAGTCCCCA GCTACGAGTA CCATGGGGAC GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508526.1
CDS	108..3206
Translation	MEEPVRELLLTNATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508463.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508463.4

ORF Insert Sequence:

ATGGAGGAGC CAGTGAGGGA GCTTCTGCTG ACCAACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGAAGTGCC GAAATGTTCC AGAAGAACCG ACCAACAAAT GGAAGCAGAG GGTTAGGACA 
GCCATGGCAG GGGTGAAATT GACCGACAAG GTGAAGCTGA CCTGGAGGGA CCGATTCCCA 
GCCTATTTCA CCAATCTTGT CTCCATCATC TTCATGATCG CAGTGACATT TGCAATCGTC 
CTCGGAGTTA TCATCTATAG AATCTCCACA GCTGCAGCCT TGGCCATGAA CTCCTCCCCG 
TCTGTGCGGT CCAACATCCG GGTTACAGTC ACGGCCACCG CTGTTATCAT CAACCTCGTG 
GTCATCATTC TGCTGGATGA AGTTTACGGC TGCATTGCCA GGTGGCTCAC CAAGATTGAG 
GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG AGGCTAACCT TCAAGGCCTT CCTGCTCAAG 
TTTGTGAACT CTTACACTCC CATCTTCTAT GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT 
CGGCCCGGTG ACTACGTGTA CATCTTCCGC TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC 
GGCTGCCTCA TGGAGCTCTG TATCCAGCTG AGCATCATTA TGCTGGGCAA GCAGCTAATC 
CAGAACAATC TCTTCGAGAT TGGCATCCCG AAGATGAAAA AGTTCATCCG CTACCTGAAG 
CTGCGCAGAC AGAGCCCCTC AGACCGTGAA GAGTACGTGA AGCGGAAGCA GCGCTATGAG 
GTGGACTTCA ACCTCGAACC TTTCGCCGGC CTCACGCCCG AGTACATGGA AATGATCATT 
CAGTTCGGCT TTGTCACCCT GTTTGTTGCG TCCTTCCCTC TGGCTCCACT CTTCGCCCTG 
CTAAACAACA TCATTGAGAT CCGCCTGGAT GCCAAAAAGT TTGTCACCGA GCTACGGAGG 
CCAGTAGCCA TCAGAGCCAA AGACATCGGC ATCTGGTATA ACATCCTCAG AGGTGTTGGG 
AAGCTGGCTG TCATCATTAA TGCCTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC 
CTGGTGTACC TCTACATGTA CAGTCAGAAT GGGACCATGC ACGGCTTCGT CAATCACACG 
CTCTCTTCCT TCAATGTCAG CGACTTCCAG AATGGCACAG CACCCAATGA CCCACTGGAC 
CTGGGCTATG AGGTTCAGAT CTGCAGGTAT AAAGATTACC GGGAACCCCC ATGGTCAGAA 
CACAAGTATG ACATCTCCAA AGACTTCTGG GCTGTCCTGG CCGCCCGACT GGCATTTGTC 
ATTGTCTTCC AGAACCTGGT GATGTTCATG AGTGACTTTG TGGACTGGGT GATCCCTGAT 
ATCCCCAAAG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TTCTCATGGT GGAGCTGTTT 
ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA CTGGACACAT GGATGGAGAA GGAGAAGCCA 
AGGGATGTGC CTTGTAACAA CCACAGCCCC ACAACCCACC CAGAGGCAGG CGACGGCAGC 
CCAGTCCCCA GCTACGAGTA CCATGGGGAC GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508463.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508526.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu40138
Clone ID Related Accession (Same CDS sequence)	XM_006508463.4 , XM_006508463.3
Accession Version	XM_006508463.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508463.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC CAGTGAGGGA GCTTCTGCTG ACCAACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGAAGTGCC GAAATGTTCC AGAAGAACCG ACCAACAAAT GGAAGCAGAG GGTTAGGACA 
GCCATGGCAG GGGTGAAATT GACCGACAAG GTGAAGCTGA CCTGGAGGGA CCGATTCCCA 
GCCTATTTCA CCAATCTTGT CTCCATCATC TTCATGATCG CAGTGACATT TGCAATCGTC 
CTCGGAGTTA TCATCTATAG AATCTCCACA GCTGCAGCCT TGGCCATGAA CTCCTCCCCG 
TCTGTGCGGT CCAACATCCG GGTTACAGTC ACGGCCACCG CTGTTATCAT CAACCTCGTG 
GTCATCATTC TGCTGGATGA AGTTTACGGC TGCATTGCCA GGTGGCTCAC CAAGATTGAG 
GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG AGGCTAACCT TCAAGGCCTT CCTGCTCAAG 
TTTGTGAACT CTTACACTCC CATCTTCTAT GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT 
CGGCCCGGTG ACTACGTGTA CATCTTCCGC TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC 
GGCTGCCTCA TGGAGCTCTG TATCCAGCTG AGCATCATTA TGCTGGGCAA GCAGCTAATC 
CAGAACAATC TCTTCGAGAT TGGCATCCCG AAGATGAAAA AGTTCATCCG CTACCTGAAG 
CTGCGCAGAC AGAGCCCCTC AGACCGTGAA GAGTACGTGA AGCGGAAGCA GCGCTATGAG 
GTGGACTTCA ACCTCGAACC TTTCGCCGGC CTCACGCCCG AGTACATGGA AATGATCATT 
CAGTTCGGCT TTGTCACCCT GTTTGTTGCG TCCTTCCCTC TGGCTCCACT CTTCGCCCTG 
CTAAACAACA TCATTGAGAT CCGCCTGGAT GCCAAAAAGT TTGTCACCGA GCTACGGAGG 
CCAGTAGCCA TCAGAGCCAA AGACATCGGC ATCTGGTATA ACATCCTCAG AGGTGTTGGG 
AAGCTGGCTG TCATCATTAA TGCCTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC 
CTGGTGTACC TCTACATGTA CAGTCAGAAT GGGACCATGC ACGGCTTCGT CAATCACACG 
CTCTCTTCCT TCAATGTCAG CGACTTCCAG AATGGCACAG CACCCAATGA CCCACTGGAC 
CTGGGCTATG AGGTTCAGAT CTGCAGGTAT AAAGATTACC GGGAACCCCC ATGGTCAGAA 
CACAAGTATG ACATCTCCAA AGACTTCTGG GCTGTCCTGG CCGCCCGACT GGCATTTGTC 
ATTGTCTTCC AGAACCTGGT GATGTTCATG AGTGACTTTG TGGACTGGGT GATCCCTGAT 
ATCCCCAAAG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TTCTCATGGT GGAGCTGTTT 
ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA CTGGACACAT GGATGGAGAA GGAGAAGCCA 
AGGGATGTGC CTTGTAACAA CCACAGCCCC ACAACCCACC CAGAGGCAGG CGACGGCAGC 
CCAGTCCCCA GCTACGAGTA CCATGGGGAC GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508526.1
CDS	127..3225
Translation	MEEPVRELLLTNATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508463.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508463.3

ORF Insert Sequence:

ATGGAGGAGC CAGTGAGGGA GCTTCTGCTG ACCAACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGAAGTGCC GAAATGTTCC AGAAGAACCG ACCAACAAAT GGAAGCAGAG GGTTAGGACA 
GCCATGGCAG GGGTGAAATT GACCGACAAG GTGAAGCTGA CCTGGAGGGA CCGATTCCCA 
GCCTATTTCA CCAATCTTGT CTCCATCATC TTCATGATCG CAGTGACATT TGCAATCGTC 
CTCGGAGTTA TCATCTATAG AATCTCCACA GCTGCAGCCT TGGCCATGAA CTCCTCCCCG 
TCTGTGCGGT CCAACATCCG GGTTACAGTC ACGGCCACCG CTGTTATCAT CAACCTCGTG 
GTCATCATTC TGCTGGATGA AGTTTACGGC TGCATTGCCA GGTGGCTCAC CAAGATTGAG 
GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG AGGCTAACCT TCAAGGCCTT CCTGCTCAAG 
TTTGTGAACT CTTACACTCC CATCTTCTAT GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT 
CGGCCCGGTG ACTACGTGTA CATCTTCCGC TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC 
GGCTGCCTCA TGGAGCTCTG TATCCAGCTG AGCATCATTA TGCTGGGCAA GCAGCTAATC 
CAGAACAATC TCTTCGAGAT TGGCATCCCG AAGATGAAAA AGTTCATCCG CTACCTGAAG 
CTGCGCAGAC AGAGCCCCTC AGACCGTGAA GAGTACGTGA AGCGGAAGCA GCGCTATGAG 
GTGGACTTCA ACCTCGAACC TTTCGCCGGC CTCACGCCCG AGTACATGGA AATGATCATT 
CAGTTCGGCT TTGTCACCCT GTTTGTTGCG TCCTTCCCTC TGGCTCCACT CTTCGCCCTG 
CTAAACAACA TCATTGAGAT CCGCCTGGAT GCCAAAAAGT TTGTCACCGA GCTACGGAGG 
CCAGTAGCCA TCAGAGCCAA AGACATCGGC ATCTGGTATA ACATCCTCAG AGGTGTTGGG 
AAGCTGGCTG TCATCATTAA TGCCTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC 
CTGGTGTACC TCTACATGTA CAGTCAGAAT GGGACCATGC ACGGCTTCGT CAATCACACG 
CTCTCTTCCT TCAATGTCAG CGACTTCCAG AATGGCACAG CACCCAATGA CCCACTGGAC 
CTGGGCTATG AGGTTCAGAT CTGCAGGTAT AAAGATTACC GGGAACCCCC ATGGTCAGAA 
CACAAGTATG ACATCTCCAA AGACTTCTGG GCTGTCCTGG CCGCCCGACT GGCATTTGTC 
ATTGTCTTCC AGAACCTGGT GATGTTCATG AGTGACTTTG TGGACTGGGT GATCCCTGAT 
ATCCCCAAAG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TTCTCATGGT GGAGCTGTTT 
ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA CTGGACACAT GGATGGAGAA GGAGAAGCCA 
AGGGATGTGC CTTGTAACAA CCACAGCCCC ACAACCCACC CAGAGGCAGG CGACGGCAGC 
CCAGTCCCCA GCTACGAGTA CCATGGGGAC GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508464.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508527.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu40139
Clone ID Related Accession (Same CDS sequence)	XM_006508464.2 , XM_006508464.3
Accession Version	XM_006508464.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006508464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAA GCTGTCAAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GAAGTGCCGA 
AATGTTCCAG AAGAACCGAC CAACAAATGG AAGCAGAGGG TTAGGACAGC CATGGCAGGG 
GTGAAATTGA CCGACAAGGT GAAGCTGACC TGGAGGGACC GATTCCCAGC CTATTTCACC 
AATCTTGTCT CCATCATCTT CATGATCGCA GTGACATTTG CAATCGTCCT CGGAGTTATC 
ATCTATAGAA TCTCCACAGC TGCAGCCTTG GCCATGAACT CCTCCCCGTC TGTGCGGTCC 
AACATCCGGG TTACAGTCAC GGCCACCGCT GTTATCATCA ACCTCGTGGT CATCATTCTG 
CTGGATGAAG TTTACGGCTG CATTGCCAGG TGGCTCACCA AGATTGAGGT CCCAAAGACA 
GAGAAGAGCT TTGAGGAGAG GCTAACCTTC AAGGCCTTCC TGCTCAAGTT TGTGAACTCT 
TACACTCCCA TCTTCTATGT CGCCTTCTTC AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC 
TACGTGTACA TCTTCCGCTC TTTCCGGATG GAGGAGTGTG CCCCGGGCGG CTGCCTCATG 
GAGCTCTGTA TCCAGCTGAG CATCATTATG CTGGGCAAGC AGCTAATCCA GAACAATCTC 
TTCGAGATTG GCATCCCGAA GATGAAAAAG TTCATCCGCT ACCTGAAGCT GCGCAGACAG 
AGCCCCTCAG ACCGTGAAGA GTACGTGAAG CGGAAGCAGC GCTATGAGGT GGACTTCAAC 
CTCGAACCTT TCGCCGGCCT CACGCCCGAG TACATGGAAA TGATCATTCA GTTCGGCTTT 
GTCACCCTGT TTGTTGCGTC CTTCCCTCTG GCTCCACTCT TCGCCCTGCT AAACAACATC 
ATTGAGATCC GCCTGGATGC CAAAAAGTTT GTCACCGAGC TACGGAGGCC AGTAGCCATC 
AGAGCCAAAG ACATCGGCAT CTGGTATAAC ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC 
ATCATTAATG CCTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC 
TACATGTACA GTCAGAATGG GACCATGCAC GGCTTCGTCA ATCACACGCT CTCTTCCTTC 
AATGTCAGCG ACTTCCAGAA TGGCACAGCA CCCAATGACC CACTGGACCT GGGCTATGAG 
GTTCAGATCT GCAGGTATAA AGATTACCGG GAACCCCCAT GGTCAGAACA CAAGTATGAC 
ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC GCCCGACTGG CATTTGTCAT TGTCTTCCAG 
AACCTGGTGA TGTTCATGAG TGACTTTGTG GACTGGGTGA TCCCTGATAT CCCCAAAGAC 
ATCAGCCAGC AGATCCACAA AGAGAAGGTT CTCATGGTGG AGCTGTTTAT GCGTGAGGAG 
CAGGGCAAGC AGCAGCTACT GGACACATGG ATGGAGAAGG AGAAGCCAAG GGATGTGCCT 
TGTAACAACC ACAGCCCCAC AACCCACCCA GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC 
TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508527.1
CDS	111..3137
Translation	MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508464.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508464.2

ORF Insert Sequence:

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAA GCTGTCAAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GAAGTGCCGA 
AATGTTCCAG AAGAACCGAC CAACAAATGG AAGCAGAGGG TTAGGACAGC CATGGCAGGG 
GTGAAATTGA CCGACAAGGT GAAGCTGACC TGGAGGGACC GATTCCCAGC CTATTTCACC 
AATCTTGTCT CCATCATCTT CATGATCGCA GTGACATTTG CAATCGTCCT CGGAGTTATC 
ATCTATAGAA TCTCCACAGC TGCAGCCTTG GCCATGAACT CCTCCCCGTC TGTGCGGTCC 
AACATCCGGG TTACAGTCAC GGCCACCGCT GTTATCATCA ACCTCGTGGT CATCATTCTG 
CTGGATGAAG TTTACGGCTG CATTGCCAGG TGGCTCACCA AGATTGAGGT CCCAAAGACA 
GAGAAGAGCT TTGAGGAGAG GCTAACCTTC AAGGCCTTCC TGCTCAAGTT TGTGAACTCT 
TACACTCCCA TCTTCTATGT CGCCTTCTTC AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC 
TACGTGTACA TCTTCCGCTC TTTCCGGATG GAGGAGTGTG CCCCGGGCGG CTGCCTCATG 
GAGCTCTGTA TCCAGCTGAG CATCATTATG CTGGGCAAGC AGCTAATCCA GAACAATCTC 
TTCGAGATTG GCATCCCGAA GATGAAAAAG TTCATCCGCT ACCTGAAGCT GCGCAGACAG 
AGCCCCTCAG ACCGTGAAGA GTACGTGAAG CGGAAGCAGC GCTATGAGGT GGACTTCAAC 
CTCGAACCTT TCGCCGGCCT CACGCCCGAG TACATGGAAA TGATCATTCA GTTCGGCTTT 
GTCACCCTGT TTGTTGCGTC CTTCCCTCTG GCTCCACTCT TCGCCCTGCT AAACAACATC 
ATTGAGATCC GCCTGGATGC CAAAAAGTTT GTCACCGAGC TACGGAGGCC AGTAGCCATC 
AGAGCCAAAG ACATCGGCAT CTGGTATAAC ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC 
ATCATTAATG CCTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC 
TACATGTACA GTCAGAATGG GACCATGCAC GGCTTCGTCA ATCACACGCT CTCTTCCTTC 
AATGTCAGCG ACTTCCAGAA TGGCACAGCA CCCAATGACC CACTGGACCT GGGCTATGAG 
GTTCAGATCT GCAGGTATAA AGATTACCGG GAACCCCCAT GGTCAGAACA CAAGTATGAC 
ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC GCCCGACTGG CATTTGTCAT TGTCTTCCAG 
AACCTGGTGA TGTTCATGAG TGACTTTGTG GACTGGGTGA TCCCTGATAT CCCCAAAGAC 
ATCAGCCAGC AGATCCACAA AGAGAAGGTT CTCATGGTGG AGCTGTTTAT GCGTGAGGAG 
CAGGGCAAGC AGCAGCTACT GGACACATGG ATGGAGAAGG AGAAGCCAAG GGATGTGCCT 
TGTAACAACC ACAGCCCCAC AACCCACCCA GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC 
TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006508464.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508527.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu40139
Clone ID Related Accession (Same CDS sequence)	XM_006508464.2 , XM_006508464.3
Accession Version	XM_006508464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAA GCTGTCAAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GAAGTGCCGA 
AATGTTCCAG AAGAACCGAC CAACAAATGG AAGCAGAGGG TTAGGACAGC CATGGCAGGG 
GTGAAATTGA CCGACAAGGT GAAGCTGACC TGGAGGGACC GATTCCCAGC CTATTTCACC 
AATCTTGTCT CCATCATCTT CATGATCGCA GTGACATTTG CAATCGTCCT CGGAGTTATC 
ATCTATAGAA TCTCCACAGC TGCAGCCTTG GCCATGAACT CCTCCCCGTC TGTGCGGTCC 
AACATCCGGG TTACAGTCAC GGCCACCGCT GTTATCATCA ACCTCGTGGT CATCATTCTG 
CTGGATGAAG TTTACGGCTG CATTGCCAGG TGGCTCACCA AGATTGAGGT CCCAAAGACA 
GAGAAGAGCT TTGAGGAGAG GCTAACCTTC AAGGCCTTCC TGCTCAAGTT TGTGAACTCT 
TACACTCCCA TCTTCTATGT CGCCTTCTTC AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC 
TACGTGTACA TCTTCCGCTC TTTCCGGATG GAGGAGTGTG CCCCGGGCGG CTGCCTCATG 
GAGCTCTGTA TCCAGCTGAG CATCATTATG CTGGGCAAGC AGCTAATCCA GAACAATCTC 
TTCGAGATTG GCATCCCGAA GATGAAAAAG TTCATCCGCT ACCTGAAGCT GCGCAGACAG 
AGCCCCTCAG ACCGTGAAGA GTACGTGAAG CGGAAGCAGC GCTATGAGGT GGACTTCAAC 
CTCGAACCTT TCGCCGGCCT CACGCCCGAG TACATGGAAA TGATCATTCA GTTCGGCTTT 
GTCACCCTGT TTGTTGCGTC CTTCCCTCTG GCTCCACTCT TCGCCCTGCT AAACAACATC 
ATTGAGATCC GCCTGGATGC CAAAAAGTTT GTCACCGAGC TACGGAGGCC AGTAGCCATC 
AGAGCCAAAG ACATCGGCAT CTGGTATAAC ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC 
ATCATTAATG CCTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC 
TACATGTACA GTCAGAATGG GACCATGCAC GGCTTCGTCA ATCACACGCT CTCTTCCTTC 
AATGTCAGCG ACTTCCAGAA TGGCACAGCA CCCAATGACC CACTGGACCT GGGCTATGAG 
GTTCAGATCT GCAGGTATAA AGATTACCGG GAACCCCCAT GGTCAGAACA CAAGTATGAC 
ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC GCCCGACTGG CATTTGTCAT TGTCTTCCAG 
AACCTGGTGA TGTTCATGAG TGACTTTGTG GACTGGGTGA TCCCTGATAT CCCCAAAGAC 
ATCAGCCAGC AGATCCACAA AGAGAAGGTT CTCATGGTGG AGCTGTTTAT GCGTGAGGAG 
CAGGGCAAGC AGCAGCTACT GGACACATGG ATGGAGAAGG AGAAGCCAAG GGATGTGCCT 
TGTAACAACC ACAGCCCCAC AACCCACCCA GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC 
TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508527.1
CDS	110..3136
Translation	MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_006508464.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508464.3

ORF Insert Sequence:

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAA GCTGTCAAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GAAGTGCCGA 
AATGTTCCAG AAGAACCGAC CAACAAATGG AAGCAGAGGG TTAGGACAGC CATGGCAGGG 
GTGAAATTGA CCGACAAGGT GAAGCTGACC TGGAGGGACC GATTCCCAGC CTATTTCACC 
AATCTTGTCT CCATCATCTT CATGATCGCA GTGACATTTG CAATCGTCCT CGGAGTTATC 
ATCTATAGAA TCTCCACAGC TGCAGCCTTG GCCATGAACT CCTCCCCGTC TGTGCGGTCC 
AACATCCGGG TTACAGTCAC GGCCACCGCT GTTATCATCA ACCTCGTGGT CATCATTCTG 
CTGGATGAAG TTTACGGCTG CATTGCCAGG TGGCTCACCA AGATTGAGGT CCCAAAGACA 
GAGAAGAGCT TTGAGGAGAG GCTAACCTTC AAGGCCTTCC TGCTCAAGTT TGTGAACTCT 
TACACTCCCA TCTTCTATGT CGCCTTCTTC AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC 
TACGTGTACA TCTTCCGCTC TTTCCGGATG GAGGAGTGTG CCCCGGGCGG CTGCCTCATG 
GAGCTCTGTA TCCAGCTGAG CATCATTATG CTGGGCAAGC AGCTAATCCA GAACAATCTC 
TTCGAGATTG GCATCCCGAA GATGAAAAAG TTCATCCGCT ACCTGAAGCT GCGCAGACAG 
AGCCCCTCAG ACCGTGAAGA GTACGTGAAG CGGAAGCAGC GCTATGAGGT GGACTTCAAC 
CTCGAACCTT TCGCCGGCCT CACGCCCGAG TACATGGAAA TGATCATTCA GTTCGGCTTT 
GTCACCCTGT TTGTTGCGTC CTTCCCTCTG GCTCCACTCT TCGCCCTGCT AAACAACATC 
ATTGAGATCC GCCTGGATGC CAAAAAGTTT GTCACCGAGC TACGGAGGCC AGTAGCCATC 
AGAGCCAAAG ACATCGGCAT CTGGTATAAC ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC 
ATCATTAATG CCTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC 
TACATGTACA GTCAGAATGG GACCATGCAC GGCTTCGTCA ATCACACGCT CTCTTCCTTC 
AATGTCAGCG ACTTCCAGAA TGGCACAGCA CCCAATGACC CACTGGACCT GGGCTATGAG 
GTTCAGATCT GCAGGTATAA AGATTACCGG GAACCCCCAT GGTCAGAACA CAAGTATGAC 
ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC GCCCGACTGG CATTTGTCAT TGTCTTCCAG 
AACCTGGTGA TGTTCATGAG TGACTTTGTG GACTGGGTGA TCCCTGATAT CCCCAAAGAC 
ATCAGCCAGC AGATCCACAA AGAGAAGGTT CTCATGGTGG AGCTGTTTAT GCGTGAGGAG 
CAGGGCAAGC AGCAGCTACT GGACACATGG ATGGAGAAGG AGAAGCCAAG GGATGTGCCT 
TGTAACAACC ACAGCCCCAC AACCCACCCA GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC 
TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_011241973.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240275.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72035
Clone ID Related Accession (Same CDS sequence)	XM_011241973.2
Accession Version	XM_011241973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA AGTGCCGAAA TGTTCCAGAA 
GAACCGACCA ACAAATGGAA GCAGAGGGTT AGGACAGCCA TGGCAGGGGT GAAATTGACC 
GACAAGGTGA AGCTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTCCTCG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCGTCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACCGCTGT TATCATCAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CAAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCTTA CACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CCGGTGACTA CGTGTACATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCGGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATTATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATTGGC 
ATCCCGAAGA TGAAAAAGTT CATCCGCTAC CTGAAGCTGC GCAGACAGAG CCCCTCAGAC 
CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCTTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTTGT CACCCTGTTT 
GTTGCGTCCT TCCCTCTGGC TCCACTCTTC GCCCTGCTAA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGGCCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT CCTCAGAGGT GTTGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT 
CAGAATGGGA CCATGCACGG CTTCGTCAAT CACACGCTCT CTTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTATGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA ACCCCCATGG TCAGAACACA AGTATGACAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGACTGGCA TTTGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAAG AGAAGGTTCT CATGGTGGAG CTGTTTATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGCCCCACAA CCCACCCAGA GGCAGGCGAC GGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240275.1
CDS	180..3194
Translation	MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_011241973.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241973.2

ORF Insert Sequence:

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAGGA AGGACTCTGC CCTTCTAAGT 
AAACGGCGGA AGTGTGGGAA ATACGGTATC ACCAGCCTCC TGGCCAATGG CGTATACTCA 
GCTGCATACC CTCTGCACGA TGGGGACTAT GAGGGTGACA ACGTTGAGTT CAACGACAGG 
AAACTCCTGT ATGAGGAATG GGCAAGTTAC GGAGTCTTCT ACAAATACCA GCCCATTGAC 
CTGGTCAGGA AATACTTTGG TGAGAAGGTT GGCCTGTACT TTGCCTGGCT TGGAGCCTAC 
ACCCAGATGC TCATCCCTGC CTCGATCGTG GGTGTCATTG TCTTTCTCTA TGGATGTGCC 
ACTGTGGACG AAAACATCCC CAGTATGGAG ATGTGTGACC AGAGATACAA CATCACCATG 
TGTCCTCTGT GTGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG TGCCACAGCC 
CGTGCCAGTC ACCTTTTTGA TAACCCTGCC ACCGTCTTCT TCTCTGTGTT TATGGCCCTC 
TGGGCTGCCA CTTTCATGGA GCACTGGAAA CGGAAGCAGA TGAGGCTCAA CTACCGATGG 
GACCTCACAG GCTTCGAGGA GGAGGAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA AGTGCCGAAA TGTTCCAGAA 
GAACCGACCA ACAAATGGAA GCAGAGGGTT AGGACAGCCA TGGCAGGGGT GAAATTGACC 
GACAAGGTGA AGCTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTCCTCG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCGTCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACCGCTGT TATCATCAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CAAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCTTA CACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CCGGTGACTA CGTGTACATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCGGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATTATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATTGGC 
ATCCCGAAGA TGAAAAAGTT CATCCGCTAC CTGAAGCTGC GCAGACAGAG CCCCTCAGAC 
CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCTTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTTGT CACCCTGTTT 
GTTGCGTCCT TCCCTCTGGC TCCACTCTTC GCCCTGCTAA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGGCCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT CCTCAGAGGT GTTGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT 
CAGAATGGGA CCATGCACGG CTTCGTCAAT CACACGCTCT CTTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTATGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA ACCCCCATGG TCAGAACACA AGTATGACAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGACTGGCA TTTGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAAG AGAAGGTTCT CATGGTGGAG CTGTTTATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGCCCCACAA CCCACCCAGA GGCAGGCGAC GGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_017321913.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177402.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X9, mRNA.

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CloneID	OMu81888
Clone ID Related Accession (Same CDS sequence)	XM_017321913.1 , XM_017321913.2
Accession Version	XM_017321913.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177402.1
CDS	147..3269
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_017321913.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321913.1

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_017321913.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177402.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu81888
Clone ID Related Accession (Same CDS sequence)	XM_017321913.1 , XM_017321913.2
Accession Version	XM_017321913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177402.1
CDS	143..3265
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_017321913.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321913.2

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241952.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097812.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89309
Clone ID Related Accession (Same CDS sequence)	XM_030241952.1
Accession Version	XM_030241952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGAAGTGCC GAAATGTTCC AGAAGAACCG ACCAACAAAT GGAAGCAGAG GGTTAGGACA 
GCCATGGCAG GGGTGAAATT GACCGACAAG GTGAAGCTGA CCTGGAGGGA CCGATTCCCA 
GCCTATTTCA CCAATCTTGT CTCCATCATC TTCATGATCG CAGTGACATT TGCAATCGTC 
CTCGGAGTTA TCATCTATAG AATCTCCACA GCTGCAGCCT TGGCCATGAA CTCCTCCCCG 
TCTGTGCGGT CCAACATCCG GGTTACAGTC ACGGCCACCG CTGTTATCAT CAACCTCGTG 
GTCATCATTC TGCTGGATGA AGTTTACGGC TGCATTGCCA GGTGGCTCAC CAAGATTGAG 
GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG AGGCTAACCT TCAAGGCCTT CCTGCTCAAG 
TTTGTGAACT CTTACACTCC CATCTTCTAT GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT 
CGGCCCGGTG ACTACGTGTA CATCTTCCGC TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC 
GGCTGCCTCA TGGAGCTCTG TATCCAGCTG AGCATCATTA TGCTGGGCAA GCAGCTAATC 
CAGAACAATC TCTTCGAGAT TGGCATCCCG AAGATGAAAA AGTTCATCCG CTACCTGAAG 
CTGCGCAGAC AGAGCCCCTC AGACCGTGAA GAGTACGTGA AGCGGAAGCA GCGCTATGAG 
GTGGACTTCA ACCTCGAACC TTTCGCCGGC CTCACGCCCG AGTACATGGA AATGATCATT 
CAGTTCGGCT TTGTCACCCT GTTTGTTGCG TCCTTCCCTC TGGCTCCACT CTTCGCCCTG 
CTAAACAACA TCATTGAGAT CCGCCTGGAT GCCAAAAAGT TTGTCACCGA GCTACGGAGG 
CCAGTAGCCA TCAGAGCCAA AGACATCGGC ATCTGGTATA ACATCCTCAG AGGTGTTGGG 
AAGCTGGCTG TCATCATTAA TGCCTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC 
CTGGTGTACC TCTACATGTA CAGTCAGAAT GGGACCATGC ACGGCTTCGT CAATCACACG 
CTCTCTTCCT TCAATGTCAG CGACTTCCAG AATGGCACAG CACCCAATGA CCCACTGGAC 
CTGGGCTATG AGGTTCAGAT CTGCAGGTAT AAAGATTACC GGGAACCCCC ATGGTCAGAA 
CACAAGTATG ACATCTCCAA AGACTTCTGG GCTGTCCTGG CCGCCCGACT GGCATTTGTC 
ATTGTCTTCC AGAACCTGGT GATGTTCATG AGTGACTTTG TGGACTGGGT GATCCCTGAT 
ATCCCCAAAG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TTCTCATGGT GGAGCTGTTT 
ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA CTGGACACAT GGATGGAGAA GGAGAAGCCA 
AGGGATGTGC CTTGTAACAA CCACAGCCCC ACAACCCACC CAGAGGCAGG CGACGGCAGC 
CCAGTCCCCA GCTACGAGTA CCATGGGGAC GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097812.1
CDS	30..3308
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241952.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241952.1

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGAAGTGCC GAAATGTTCC AGAAGAACCG ACCAACAAAT GGAAGCAGAG GGTTAGGACA 
GCCATGGCAG GGGTGAAATT GACCGACAAG GTGAAGCTGA CCTGGAGGGA CCGATTCCCA 
GCCTATTTCA CCAATCTTGT CTCCATCATC TTCATGATCG CAGTGACATT TGCAATCGTC 
CTCGGAGTTA TCATCTATAG AATCTCCACA GCTGCAGCCT TGGCCATGAA CTCCTCCCCG 
TCTGTGCGGT CCAACATCCG GGTTACAGTC ACGGCCACCG CTGTTATCAT CAACCTCGTG 
GTCATCATTC TGCTGGATGA AGTTTACGGC TGCATTGCCA GGTGGCTCAC CAAGATTGAG 
GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG AGGCTAACCT TCAAGGCCTT CCTGCTCAAG 
TTTGTGAACT CTTACACTCC CATCTTCTAT GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT 
CGGCCCGGTG ACTACGTGTA CATCTTCCGC TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC 
GGCTGCCTCA TGGAGCTCTG TATCCAGCTG AGCATCATTA TGCTGGGCAA GCAGCTAATC 
CAGAACAATC TCTTCGAGAT TGGCATCCCG AAGATGAAAA AGTTCATCCG CTACCTGAAG 
CTGCGCAGAC AGAGCCCCTC AGACCGTGAA GAGTACGTGA AGCGGAAGCA GCGCTATGAG 
GTGGACTTCA ACCTCGAACC TTTCGCCGGC CTCACGCCCG AGTACATGGA AATGATCATT 
CAGTTCGGCT TTGTCACCCT GTTTGTTGCG TCCTTCCCTC TGGCTCCACT CTTCGCCCTG 
CTAAACAACA TCATTGAGAT CCGCCTGGAT GCCAAAAAGT TTGTCACCGA GCTACGGAGG 
CCAGTAGCCA TCAGAGCCAA AGACATCGGC ATCTGGTATA ACATCCTCAG AGGTGTTGGG 
AAGCTGGCTG TCATCATTAA TGCCTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC 
CTGGTGTACC TCTACATGTA CAGTCAGAAT GGGACCATGC ACGGCTTCGT CAATCACACG 
CTCTCTTCCT TCAATGTCAG CGACTTCCAG AATGGCACAG CACCCAATGA CCCACTGGAC 
CTGGGCTATG AGGTTCAGAT CTGCAGGTAT AAAGATTACC GGGAACCCCC ATGGTCAGAA 
CACAAGTATG ACATCTCCAA AGACTTCTGG GCTGTCCTGG CCGCCCGACT GGCATTTGTC 
ATTGTCTTCC AGAACCTGGT GATGTTCATG AGTGACTTTG TGGACTGGGT GATCCCTGAT 
ATCCCCAAAG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TTCTCATGGT GGAGCTGTTT 
ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA CTGGACACAT GGATGGAGAA GGAGAAGCCA 
AGGGATGTGC CTTGTAACAA CCACAGCCCC ACAACCCACC CAGAGGCAGG CGACGGCAGC 
CCAGTCCCCA GCTACGAGTA CCATGGGGAC GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_011241973.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240275.2 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu89310
Clone ID Related Accession (Same CDS sequence)	XM_011241973.3
Accession Version	XM_011241973.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241973.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GAAGTGCCGA 
AATGTTCCAG AAGAACCGAC CAACAAATGG AAGCAGAGGG TTAGGACAGC CATGGCAGGG 
GTGAAATTGA CCGACAAGGT GAAGCTGACC TGGAGGGACC GATTCCCAGC CTATTTCACC 
AATCTTGTCT CCATCATCTT CATGATCGCA GTGACATTTG CAATCGTCCT CGGAGTTATC 
ATCTATAGAA TCTCCACAGC TGCAGCCTTG GCCATGAACT CCTCCCCGTC TGTGCGGTCC 
AACATCCGGG TTACAGTCAC GGCCACCGCT GTTATCATCA ACCTCGTGGT CATCATTCTG 
CTGGATGAAG TTTACGGCTG CATTGCCAGG TGGCTCACCA AGATTGAGGT CCCAAAGACA 
GAGAAGAGCT TTGAGGAGAG GCTAACCTTC AAGGCCTTCC TGCTCAAGTT TGTGAACTCT 
TACACTCCCA TCTTCTATGT CGCCTTCTTC AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC 
TACGTGTACA TCTTCCGCTC TTTCCGGATG GAGGAGTGTG CCCCGGGCGG CTGCCTCATG 
GAGCTCTGTA TCCAGCTGAG CATCATTATG CTGGGCAAGC AGCTAATCCA GAACAATCTC 
TTCGAGATTG GCATCCCGAA GATGAAAAAG TTCATCCGCT ACCTGAAGCT GCGCAGACAG 
AGCCCCTCAG ACCGTGAAGA GTACGTGAAG CGGAAGCAGC GCTATGAGGT GGACTTCAAC 
CTCGAACCTT TCGCCGGCCT CACGCCCGAG TACATGGAAA TGATCATTCA GTTCGGCTTT 
GTCACCCTGT TTGTTGCGTC CTTCCCTCTG GCTCCACTCT TCGCCCTGCT AAACAACATC 
ATTGAGATCC GCCTGGATGC CAAAAAGTTT GTCACCGAGC TACGGAGGCC AGTAGCCATC 
AGAGCCAAAG ACATCGGCAT CTGGTATAAC ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC 
ATCATTAATG CCTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC 
TACATGTACA GTCAGAATGG GACCATGCAC GGCTTCGTCA ATCACACGCT CTCTTCCTTC 
AATGTCAGCG ACTTCCAGAA TGGCACAGCA CCCAATGACC CACTGGACCT GGGCTATGAG 
GTTCAGATCT GCAGGTATAA AGATTACCGG GAACCCCCAT GGTCAGAACA CAAGTATGAC 
ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC GCCCGACTGG CATTTGTCAT TGTCTTCCAG 
AACCTGGTGA TGTTCATGAG TGACTTTGTG GACTGGGTGA TCCCTGATAT CCCCAAAGAC 
ATCAGCCAGC AGATCCACAA AGAGAAGGTT CTCATGGTGG AGCTGTTTAT GCGTGAGGAG 
CAGGGCAAGC AGCAGCTACT GGACACATGG ATGGAGAAGG AGAAGCCAAG GGATGTGCCT 
TGTAACAACC ACAGCCCCAC AACCCACCCA GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC 
TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240275.2
CDS	30..3296
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_011241973.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241973.3

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GAAGTGCCGA 
AATGTTCCAG AAGAACCGAC CAACAAATGG AAGCAGAGGG TTAGGACAGC CATGGCAGGG 
GTGAAATTGA CCGACAAGGT GAAGCTGACC TGGAGGGACC GATTCCCAGC CTATTTCACC 
AATCTTGTCT CCATCATCTT CATGATCGCA GTGACATTTG CAATCGTCCT CGGAGTTATC 
ATCTATAGAA TCTCCACAGC TGCAGCCTTG GCCATGAACT CCTCCCCGTC TGTGCGGTCC 
AACATCCGGG TTACAGTCAC GGCCACCGCT GTTATCATCA ACCTCGTGGT CATCATTCTG 
CTGGATGAAG TTTACGGCTG CATTGCCAGG TGGCTCACCA AGATTGAGGT CCCAAAGACA 
GAGAAGAGCT TTGAGGAGAG GCTAACCTTC AAGGCCTTCC TGCTCAAGTT TGTGAACTCT 
TACACTCCCA TCTTCTATGT CGCCTTCTTC AAAGGCCGGT TTGTTGGTCG GCCCGGTGAC 
TACGTGTACA TCTTCCGCTC TTTCCGGATG GAGGAGTGTG CCCCGGGCGG CTGCCTCATG 
GAGCTCTGTA TCCAGCTGAG CATCATTATG CTGGGCAAGC AGCTAATCCA GAACAATCTC 
TTCGAGATTG GCATCCCGAA GATGAAAAAG TTCATCCGCT ACCTGAAGCT GCGCAGACAG 
AGCCCCTCAG ACCGTGAAGA GTACGTGAAG CGGAAGCAGC GCTATGAGGT GGACTTCAAC 
CTCGAACCTT TCGCCGGCCT CACGCCCGAG TACATGGAAA TGATCATTCA GTTCGGCTTT 
GTCACCCTGT TTGTTGCGTC CTTCCCTCTG GCTCCACTCT TCGCCCTGCT AAACAACATC 
ATTGAGATCC GCCTGGATGC CAAAAAGTTT GTCACCGAGC TACGGAGGCC AGTAGCCATC 
AGAGCCAAAG ACATCGGCAT CTGGTATAAC ATCCTCAGAG GTGTTGGGAA GCTGGCTGTC 
ATCATTAATG CCTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC 
TACATGTACA GTCAGAATGG GACCATGCAC GGCTTCGTCA ATCACACGCT CTCTTCCTTC 
AATGTCAGCG ACTTCCAGAA TGGCACAGCA CCCAATGACC CACTGGACCT GGGCTATGAG 
GTTCAGATCT GCAGGTATAA AGATTACCGG GAACCCCCAT GGTCAGAACA CAAGTATGAC 
ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC GCCCGACTGG CATTTGTCAT TGTCTTCCAG 
AACCTGGTGA TGTTCATGAG TGACTTTGTG GACTGGGTGA TCCCTGATAT CCCCAAAGAC 
ATCAGCCAGC AGATCCACAA AGAGAAGGTT CTCATGGTGG AGCTGTTTAT GCGTGAGGAG 
CAGGGCAAGC AGCAGCTACT GGACACATGG ATGGAGAAGG AGAAGCCAAG GGATGTGCCT 
TGTAACAACC ACAGCCCCAC AACCCACCCA GAGGCAGGCG ACGGCAGCCC AGTCCCCAGC 
TACGAGTACC ATGGGGACGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241953.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097813.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu89311
Clone ID Related Accession (Same CDS sequence)	XM_030241953.1
Accession Version	XM_030241953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTGA GTACCCAGTT TTCTGCTACC CTGGCGGAGC CGGCGCGCGG CCAAGTTCAC 
GGCGCGCGGG CGCCCTCGGG ACGGGCCAGG TGGCGGCAGC GCCCGGGAGG TCGCGAGGGA 
GGAGGGAGCG GCCACGTCCC GTCCGCGCCA GGCCAGCACC GCTTCTCCGT TGCAGACGCC 
ACCGGGGTTG TGGATGGGGA GCGCGAGGCC ACCATGAGGG TCCCCGAGAA GTACTCGACG 
CTCCCGGCGG AGGACCGCAG CGTCCACATC GTGAACATCT GCGCCATCGA GGACCTGGGC 
TACCTGCCGT CCGAGGGCAC GTTGCTGAAC TCTCTGTCCG TGGACCCCGA CGCCGAATGC 
AAGTATGGAC TATACTTCAG GGATGGCAAA CGGAAGGTGG ACTACATCTT GGTATACCAT 
CACAAGAGAG CCTCAGGGAG CAGGACTCTG GCCAGGAGGG GACTACAAAA TGACATGGTC 
CTGGGGACCC GCAGCGTCAG GCAGGACCAG CCCCTTCCCG GGAAGGGGAG CCCTGTGGAT 
GCAGGCTCAC CGGAAGTCCC CATGGATTAC CATGAAGATG ACAAACGCTT CAGACGGGAG 
GAATATGAGG GCAACCTGTT GGAGGCAGGC CTGGAGTTGG AGAATGACGA GGATACCAAA 
ATCCATGGTG TCGGGTTTGT GAAGATCCAT GCGCCCTGGC ATGTGCTCTG TAGGGAAGCT 
GAGTTTTTGA AACTAAAGAT GCCCACAAAG AAGGTGTACC ACATCAGTGA GACGCGAGGC 
CTCCTGAAAA CCATCAACTC GGTTCTGCAG AAGATCACAG ACCCCATCCA GCCCAAGGTG 
GCTGAGCACA GGCCACAGAC CACAAAGAGG CTCTCCTATC CCTTCTCCCG GGAGAAGCAA 
CACCTATTCG ACCTGACTGA CAGGGACTCT TTTTTCGACA GCAAAACCCG GAGCACAATA 
GTCTATGAAA TCCTGAAGAG AACAACGTGC ACCAAGGCCA AGTACAGCAT GGGGCAAGGA 
GAGGGAAGAA GGAAGGACTC TGCCCTTCTA AGTAAACGGC GGAAGTGTGG GAAATACGGT 
ATCACCAGCC TCCTGGCCAA TGGCGTATAC TCAGCTGCAT ACCCTCTGCA CGATGGGGAC 
TATGAGGGTG ACAACGTTGA GTTCAACGAC AGGAAACTCC TGTATGAGGA ATGGGCAAGT 
TACGGAGTCT TCTACAAATA CCAGCCCATT GACCTGGTCA GGAAATACTT TGGTGAGAAG 
GTTGGCCTGT ACTTTGCCTG GCTTGGAGCC TACACCCAGA TGCTCATCCC TGCCTCGATC 
GTGGGTGTCA TTGTCTTTCT CTATGGATGT GCCACTGTGG ACGAAAACAT CCCCAGTATG 
GAGATGTGTG ACCAGAGATA CAACATCACC ATGTGTCCTC TGTGTGACAA GACCTGCAGC 
TACTGGAAGA TGAGCTCAGC CTGTGCCACA GCCCGTGCCA GTCACCTTTT TGATAACCCT 
GCCACCGTCT TCTTCTCTGT GTTTATGGCC CTCTGGGCTG CCACTTTCAT GGAGCACTGG 
AAACGGAAGC AGATGAGGCT CAACTACCGA TGGGACCTCA CAGGCTTCGA GGAGGAGGAG 
GAAGCTGTCA AGGATCATCC CAGAGCAGAG TATGAAGCCA GAGTCTTAGA GAAGTCACTG 
AGAAAAGAAT CCAGAAACAA AGAGAAGTGC CGAAATGTTC CAGAAGAACC GACCAACAAA 
TGGAAGCAGA GGGTTAGGAC AGCCATGGCA GGGGTGAAAT TGACCGACAA GGTGAAGCTG 
ACCTGGAGGG ACCGATTCCC AGCCTATTTC ACCAATCTTG TCTCCATCAT CTTCATGATC 
GCAGTGACAT TTGCAATCGT CCTCGGAGTT ATCATCTATA GAATCTCCAC AGCTGCAGCC 
TTGGCCATGA ACTCCTCCCC GTCTGTGCGG TCCAACATCC GGGTTACAGT CACGGCCACC 
GCTGTTATCA TCAACCTCGT GGTCATCATT CTGCTGGATG AAGTTTACGG CTGCATTGCC 
AGGTGGCTCA CCAAGATTGA GGTCCCAAAG ACAGAGAAGA GCTTTGAGGA GAGGCTAACC 
TTCAAGGCCT TCCTGCTCAA GTTTGTGAAC TCTTACACTC CCATCTTCTA TGTCGCCTTC 
TTCAAAGGCC GGTTTGTTGG TCGGCCCGGT GACTACGTGT ACATCTTCCG CTCTTTCCGG 
ATGGAGGAGT GTGCCCCGGG CGGCTGCCTC ATGGAGCTCT GTATCCAGCT GAGCATCATT 
ATGCTGGGCA AGCAGCTAAT CCAGAACAAT CTCTTCGAGA TTGGCATCCC GAAGATGAAA 
AAGTTCATCC GCTACCTGAA GCTGCGCAGA CAGAGCCCCT CAGACCGTGA AGAGTACGTG 
AAGCGGAAGC AGCGCTATGA GGTGGACTTC AACCTCGAAC CTTTCGCCGG CCTCACGCCC 
GAGTACATGG AAATGATCAT TCAGTTCGGC TTTGTCACCC TGTTTGTTGC GTCCTTCCCT 
CTGGCTCCAC TCTTCGCCCT GCTAAACAAC ATCATTGAGA TCCGCCTGGA TGCCAAAAAG 
TTTGTCACCG AGCTACGGAG GCCAGTAGCC ATCAGAGCCA AAGACATCGG CATCTGGTAT 
AACATCCTCA GAGGTGTTGG GAAGCTGGCT GTCATCATTA ATGCCTTTGT GATCTCCTTC 
ACGTCTGACT TCATCCCTCG CCTGGTGTAC CTCTACATGT ACAGTCAGAA TGGGACCATG 
CACGGCTTCG TCAATCACAC GCTCTCTTCC TTCAATGTCA GCGACTTCCA GAATGGCACA 
GCACCCAATG ACCCACTGGA CCTGGGCTAT GAGGTTCAGA TCTGCAGGTA TAAAGATTAC 
CGGGAACCCC CATGGTCAGA ACACAAGTAT GACATCTCCA AAGACTTCTG GGCTGTCCTG 
GCCGCCCGAC TGGCATTTGT CATTGTCTTC CAGAACCTGG TGATGTTCAT GAGTGACTTT 
GTGGACTGGG TGATCCCTGA TATCCCCAAA GACATCAGCC AGCAGATCCA CAAAGAGAAG 
GTTCTCATGG TGGAGCTGTT TATGCGTGAG GAGCAGGGCA AGCAGCAGCT ACTGGACACA 
TGGATGGAGA AGGAGAAGCC AAGGGATGTG CCTTGTAACA ACCACAGCCC CACAACCCAC 
CCAGAGGCAG GCGACGGCAG CCCAGTCCCC AGCTACGAGT ACCATGGGGA CGCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097813.1
CDS	16..3255
Translation	MGLSTQFSATLAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241953.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241953.1

ORF Insert Sequence:

ATGGGCCTGA GTACCCAGTT TTCTGCTACC CTGGCGGAGC CGGCGCGCGG CCAAGTTCAC 
GGCGCGCGGG CGCCCTCGGG ACGGGCCAGG TGGCGGCAGC GCCCGGGAGG TCGCGAGGGA 
GGAGGGAGCG GCCACGTCCC GTCCGCGCCA GGCCAGCACC GCTTCTCCGT TGCAGACGCC 
ACCGGGGTTG TGGATGGGGA GCGCGAGGCC ACCATGAGGG TCCCCGAGAA GTACTCGACG 
CTCCCGGCGG AGGACCGCAG CGTCCACATC GTGAACATCT GCGCCATCGA GGACCTGGGC 
TACCTGCCGT CCGAGGGCAC GTTGCTGAAC TCTCTGTCCG TGGACCCCGA CGCCGAATGC 
AAGTATGGAC TATACTTCAG GGATGGCAAA CGGAAGGTGG ACTACATCTT GGTATACCAT 
CACAAGAGAG CCTCAGGGAG CAGGACTCTG GCCAGGAGGG GACTACAAAA TGACATGGTC 
CTGGGGACCC GCAGCGTCAG GCAGGACCAG CCCCTTCCCG GGAAGGGGAG CCCTGTGGAT 
GCAGGCTCAC CGGAAGTCCC CATGGATTAC CATGAAGATG ACAAACGCTT CAGACGGGAG 
GAATATGAGG GCAACCTGTT GGAGGCAGGC CTGGAGTTGG AGAATGACGA GGATACCAAA 
ATCCATGGTG TCGGGTTTGT GAAGATCCAT GCGCCCTGGC ATGTGCTCTG TAGGGAAGCT 
GAGTTTTTGA AACTAAAGAT GCCCACAAAG AAGGTGTACC ACATCAGTGA GACGCGAGGC 
CTCCTGAAAA CCATCAACTC GGTTCTGCAG AAGATCACAG ACCCCATCCA GCCCAAGGTG 
GCTGAGCACA GGCCACAGAC CACAAAGAGG CTCTCCTATC CCTTCTCCCG GGAGAAGCAA 
CACCTATTCG ACCTGACTGA CAGGGACTCT TTTTTCGACA GCAAAACCCG GAGCACAATA 
GTCTATGAAA TCCTGAAGAG AACAACGTGC ACCAAGGCCA AGTACAGCAT GGGGCAAGGA 
GAGGGAAGAA GGAAGGACTC TGCCCTTCTA AGTAAACGGC GGAAGTGTGG GAAATACGGT 
ATCACCAGCC TCCTGGCCAA TGGCGTATAC TCAGCTGCAT ACCCTCTGCA CGATGGGGAC 
TATGAGGGTG ACAACGTTGA GTTCAACGAC AGGAAACTCC TGTATGAGGA ATGGGCAAGT 
TACGGAGTCT TCTACAAATA CCAGCCCATT GACCTGGTCA GGAAATACTT TGGTGAGAAG 
GTTGGCCTGT ACTTTGCCTG GCTTGGAGCC TACACCCAGA TGCTCATCCC TGCCTCGATC 
GTGGGTGTCA TTGTCTTTCT CTATGGATGT GCCACTGTGG ACGAAAACAT CCCCAGTATG 
GAGATGTGTG ACCAGAGATA CAACATCACC ATGTGTCCTC TGTGTGACAA GACCTGCAGC 
TACTGGAAGA TGAGCTCAGC CTGTGCCACA GCCCGTGCCA GTCACCTTTT TGATAACCCT 
GCCACCGTCT TCTTCTCTGT GTTTATGGCC CTCTGGGCTG CCACTTTCAT GGAGCACTGG 
AAACGGAAGC AGATGAGGCT CAACTACCGA TGGGACCTCA CAGGCTTCGA GGAGGAGGAG 
GAAGCTGTCA AGGATCATCC CAGAGCAGAG TATGAAGCCA GAGTCTTAGA GAAGTCACTG 
AGAAAAGAAT CCAGAAACAA AGAGAAGTGC CGAAATGTTC CAGAAGAACC GACCAACAAA 
TGGAAGCAGA GGGTTAGGAC AGCCATGGCA GGGGTGAAAT TGACCGACAA GGTGAAGCTG 
ACCTGGAGGG ACCGATTCCC AGCCTATTTC ACCAATCTTG TCTCCATCAT CTTCATGATC 
GCAGTGACAT TTGCAATCGT CCTCGGAGTT ATCATCTATA GAATCTCCAC AGCTGCAGCC 
TTGGCCATGA ACTCCTCCCC GTCTGTGCGG TCCAACATCC GGGTTACAGT CACGGCCACC 
GCTGTTATCA TCAACCTCGT GGTCATCATT CTGCTGGATG AAGTTTACGG CTGCATTGCC 
AGGTGGCTCA CCAAGATTGA GGTCCCAAAG ACAGAGAAGA GCTTTGAGGA GAGGCTAACC 
TTCAAGGCCT TCCTGCTCAA GTTTGTGAAC TCTTACACTC CCATCTTCTA TGTCGCCTTC 
TTCAAAGGCC GGTTTGTTGG TCGGCCCGGT GACTACGTGT ACATCTTCCG CTCTTTCCGG 
ATGGAGGAGT GTGCCCCGGG CGGCTGCCTC ATGGAGCTCT GTATCCAGCT GAGCATCATT 
ATGCTGGGCA AGCAGCTAAT CCAGAACAAT CTCTTCGAGA TTGGCATCCC GAAGATGAAA 
AAGTTCATCC GCTACCTGAA GCTGCGCAGA CAGAGCCCCT CAGACCGTGA AGAGTACGTG 
AAGCGGAAGC AGCGCTATGA GGTGGACTTC AACCTCGAAC CTTTCGCCGG CCTCACGCCC 
GAGTACATGG AAATGATCAT TCAGTTCGGC TTTGTCACCC TGTTTGTTGC GTCCTTCCCT 
CTGGCTCCAC TCTTCGCCCT GCTAAACAAC ATCATTGAGA TCCGCCTGGA TGCCAAAAAG 
TTTGTCACCG AGCTACGGAG GCCAGTAGCC ATCAGAGCCA AAGACATCGG CATCTGGTAT 
AACATCCTCA GAGGTGTTGG GAAGCTGGCT GTCATCATTA ATGCCTTTGT GATCTCCTTC 
ACGTCTGACT TCATCCCTCG CCTGGTGTAC CTCTACATGT ACAGTCAGAA TGGGACCATG 
CACGGCTTCG TCAATCACAC GCTCTCTTCC TTCAATGTCA GCGACTTCCA GAATGGCACA 
GCACCCAATG ACCCACTGGA CCTGGGCTAT GAGGTTCAGA TCTGCAGGTA TAAAGATTAC 
CGGGAACCCC CATGGTCAGA ACACAAGTAT GACATCTCCA AAGACTTCTG GGCTGTCCTG 
GCCGCCCGAC TGGCATTTGT CATTGTCTTC CAGAACCTGG TGATGTTCAT GAGTGACTTT 
GTGGACTGGG TGATCCCTGA TATCCCCAAA GACATCAGCC AGCAGATCCA CAAAGAGAAG 
GTTCTCATGG TGGAGCTGTT TATGCGTGAG GAGCAGGGCA AGCAGCAGCT ACTGGACACA 
TGGATGGAGA AGGAGAAGCC AAGGGATGTG CCTTGTAACA ACCACAGCCC CACAACCCAC 
CCAGAGGCAG GCGACGGCAG CCCAGTCCCC AGCTACGAGT ACCATGGGGA CGCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_011241974.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240276.2 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu89312
Clone ID Related Accession (Same CDS sequence)	XM_011241974.3
Accession Version	XM_011241974.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGAAGCTG TCAAGGATCA TCCCAGAGCA 
GAGTATGAAG CCAGAGTCTT AGAGAAGTCA CTGAGAAAAG AATCCAGAAA CAAAGAGAAG 
TGCCGAAATG TTCCAGAAGA ACCGACCAAC AAATGGAAGC AGAGGGTTAG GACAGCCATG 
GCAGGGGTGA AATTGACCGA CAAGGTGAAG CTGACCTGGA GGGACCGATT CCCAGCCTAT 
TTCACCAATC TTGTCTCCAT CATCTTCATG ATCGCAGTGA CATTTGCAAT CGTCCTCGGA 
GTTATCATCT ATAGAATCTC CACAGCTGCA GCCTTGGCCA TGAACTCCTC CCCGTCTGTG 
CGGTCCAACA TCCGGGTTAC AGTCACGGCC ACCGCTGTTA TCATCAACCT CGTGGTCATC 
ATTCTGCTGG ATGAAGTTTA CGGCTGCATT GCCAGGTGGC TCACCAAGAT TGAGGTCCCA 
AAGACAGAGA AGAGCTTTGA GGAGAGGCTA ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG 
AACTCTTACA CTCCCATCTT CTATGTCGCC TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC 
GGTGACTACG TGTACATCTT CCGCTCTTTC CGGATGGAGG AGTGTGCCCC GGGCGGCTGC 
CTCATGGAGC TCTGTATCCA GCTGAGCATC ATTATGCTGG GCAAGCAGCT AATCCAGAAC 
AATCTCTTCG AGATTGGCAT CCCGAAGATG AAAAAGTTCA TCCGCTACCT GAAGCTGCGC 
AGACAGAGCC CCTCAGACCG TGAAGAGTAC GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC 
TTCAACCTCG AACCTTTCGC CGGCCTCACG CCCGAGTACA TGGAAATGAT CATTCAGTTC 
GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC CCTCTGGCTC CACTCTTCGC CCTGCTAAAC 
AACATCATTG AGATCCGCCT GGATGCCAAA AAGTTTGTCA CCGAGCTACG GAGGCCAGTA 
GCCATCAGAG CCAAAGACAT CGGCATCTGG TATAACATCC TCAGAGGTGT TGGGAAGCTG 
GCTGTCATCA TTAATGCCTT TGTGATCTCC TTCACGTCTG ACTTCATCCC TCGCCTGGTG 
TACCTCTACA TGTACAGTCA GAATGGGACC ATGCACGGCT TCGTCAATCA CACGCTCTCT 
TCCTTCAATG TCAGCGACTT CCAGAATGGC ACAGCACCCA ATGACCCACT GGACCTGGGC 
TATGAGGTTC AGATCTGCAG GTATAAAGAT TACCGGGAAC CCCCATGGTC AGAACACAAG 
TATGACATCT CCAAAGACTT CTGGGCTGTC CTGGCCGCCC GACTGGCATT TGTCATTGTC 
TTCCAGAACC TGGTGATGTT CATGAGTGAC TTTGTGGACT GGGTGATCCC TGATATCCCC 
AAAGACATCA GCCAGCAGAT CCACAAAGAG AAGGTTCTCA TGGTGGAGCT GTTTATGCGT 
GAGGAGCAGG GCAAGCAGCA GCTACTGGAC ACATGGATGG AGAAGGAGAA GCCAAGGGAT 
GTGCCTTGTA ACAACCACAG CCCCACAACC CACCCAGAGG CAGGCGACGG CAGCCCAGTC 
CCCAGCTACG AGTACCATGG GGACGCGCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240276.2
CDS	29..3241
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_011241974.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241974.3

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGAAGCTG TCAAGGATCA TCCCAGAGCA 
GAGTATGAAG CCAGAGTCTT AGAGAAGTCA CTGAGAAAAG AATCCAGAAA CAAAGAGAAG 
TGCCGAAATG TTCCAGAAGA ACCGACCAAC AAATGGAAGC AGAGGGTTAG GACAGCCATG 
GCAGGGGTGA AATTGACCGA CAAGGTGAAG CTGACCTGGA GGGACCGATT CCCAGCCTAT 
TTCACCAATC TTGTCTCCAT CATCTTCATG ATCGCAGTGA CATTTGCAAT CGTCCTCGGA 
GTTATCATCT ATAGAATCTC CACAGCTGCA GCCTTGGCCA TGAACTCCTC CCCGTCTGTG 
CGGTCCAACA TCCGGGTTAC AGTCACGGCC ACCGCTGTTA TCATCAACCT CGTGGTCATC 
ATTCTGCTGG ATGAAGTTTA CGGCTGCATT GCCAGGTGGC TCACCAAGAT TGAGGTCCCA 
AAGACAGAGA AGAGCTTTGA GGAGAGGCTA ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG 
AACTCTTACA CTCCCATCTT CTATGTCGCC TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC 
GGTGACTACG TGTACATCTT CCGCTCTTTC CGGATGGAGG AGTGTGCCCC GGGCGGCTGC 
CTCATGGAGC TCTGTATCCA GCTGAGCATC ATTATGCTGG GCAAGCAGCT AATCCAGAAC 
AATCTCTTCG AGATTGGCAT CCCGAAGATG AAAAAGTTCA TCCGCTACCT GAAGCTGCGC 
AGACAGAGCC CCTCAGACCG TGAAGAGTAC GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC 
TTCAACCTCG AACCTTTCGC CGGCCTCACG CCCGAGTACA TGGAAATGAT CATTCAGTTC 
GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC CCTCTGGCTC CACTCTTCGC CCTGCTAAAC 
AACATCATTG AGATCCGCCT GGATGCCAAA AAGTTTGTCA CCGAGCTACG GAGGCCAGTA 
GCCATCAGAG CCAAAGACAT CGGCATCTGG TATAACATCC TCAGAGGTGT TGGGAAGCTG 
GCTGTCATCA TTAATGCCTT TGTGATCTCC TTCACGTCTG ACTTCATCCC TCGCCTGGTG 
TACCTCTACA TGTACAGTCA GAATGGGACC ATGCACGGCT TCGTCAATCA CACGCTCTCT 
TCCTTCAATG TCAGCGACTT CCAGAATGGC ACAGCACCCA ATGACCCACT GGACCTGGGC 
TATGAGGTTC AGATCTGCAG GTATAAAGAT TACCGGGAAC CCCCATGGTC AGAACACAAG 
TATGACATCT CCAAAGACTT CTGGGCTGTC CTGGCCGCCC GACTGGCATT TGTCATTGTC 
TTCCAGAACC TGGTGATGTT CATGAGTGAC TTTGTGGACT GGGTGATCCC TGATATCCCC 
AAAGACATCA GCCAGCAGAT CCACAAAGAG AAGGTTCTCA TGGTGGAGCT GTTTATGCGT 
GAGGAGCAGG GCAAGCAGCA GCTACTGGAC ACATGGATGG AGAAGGAGAA GCCAAGGGAT 
GTGCCTTGTA ACAACCACAG CCCCACAACC CACCCAGAGG CAGGCGACGG CAGCCCAGTC 
CCCAGCTACG AGTACCATGG GGACGCGCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241954.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097814.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu89313
Clone ID Related Accession (Same CDS sequence)	XM_030241954.1
Accession Version	XM_030241954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097814.1
CDS	28..3228
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241954.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241954.1

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG AAGCTGTCAA GGATCATCCC 
AGAGCAGAGT ATGAAGCCAG AGTCTTAGAG AAGTCACTGA GAAAAGAATC CAGAAACAAA 
GAGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241955.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097815.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X7, mRNA.

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CloneID	OMu89314
Clone ID Related Accession (Same CDS sequence)	XM_030241955.1
Accession Version	XM_030241955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097815.1
CDS	29..3229
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241955.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241955.1

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_011241975.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240277.2 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu89315
Clone ID Related Accession (Same CDS sequence)	XM_011241975.3
Accession Version	XM_011241975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241975.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GACCGACAAG 
GTGAAGCTGA CCTGGAGGGA CCGATTCCCA GCCTATTTCA CCAATCTTGT CTCCATCATC 
TTCATGATCG CAGTGACATT TGCAATCGTC CTCGGAGTTA TCATCTATAG AATCTCCACA 
GCTGCAGCCT TGGCCATGAA CTCCTCCCCG TCTGTGCGGT CCAACATCCG GGTTACAGTC 
ACGGCCACCG CTGTTATCAT CAACCTCGTG GTCATCATTC TGCTGGATGA AGTTTACGGC 
TGCATTGCCA GGTGGCTCAC CAAGATTGAG GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG 
AGGCTAACCT TCAAGGCCTT CCTGCTCAAG TTTGTGAACT CTTACACTCC CATCTTCTAT 
GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT CGGCCCGGTG ACTACGTGTA CATCTTCCGC 
TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC GGCTGCCTCA TGGAGCTCTG TATCCAGCTG 
AGCATCATTA TGCTGGGCAA GCAGCTAATC CAGAACAATC TCTTCGAGAT TGGCATCCCG 
AAGATGAAAA AGTTCATCCG CTACCTGAAG CTGCGCAGAC AGAGCCCCTC AGACCGTGAA 
GAGTACGTGA AGCGGAAGCA GCGCTATGAG GTGGACTTCA ACCTCGAACC TTTCGCCGGC 
CTCACGCCCG AGTACATGGA AATGATCATT CAGTTCGGCT TTGTCACCCT GTTTGTTGCG 
TCCTTCCCTC TGGCTCCACT CTTCGCCCTG CTAAACAACA TCATTGAGAT CCGCCTGGAT 
GCCAAAAAGT TTGTCACCGA GCTACGGAGG CCAGTAGCCA TCAGAGCCAA AGACATCGGC 
ATCTGGTATA ACATCCTCAG AGGTGTTGGG AAGCTGGCTG TCATCATTAA TGCCTTTGTG 
ATCTCCTTCA CGTCTGACTT CATCCCTCGC CTGGTGTACC TCTACATGTA CAGTCAGAAT 
GGGACCATGC ACGGCTTCGT CAATCACACG CTCTCTTCCT TCAATGTCAG CGACTTCCAG 
AATGGCACAG CACCCAATGA CCCACTGGAC CTGGGCTATG AGGTTCAGAT CTGCAGGTAT 
AAAGATTACC GGGAACCCCC ATGGTCAGAA CACAAGTATG ACATCTCCAA AGACTTCTGG 
GCTGTCCTGG CCGCCCGACT GGCATTTGTC ATTGTCTTCC AGAACCTGGT GATGTTCATG 
AGTGACTTTG TGGACTGGGT GATCCCTGAT ATCCCCAAAG ACATCAGCCA GCAGATCCAC 
AAAGAGAAGG TTCTCATGGT GGAGCTGTTT ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA 
CTGGACACAT GGATGGAGAA GGAGAAGCCA AGGGATGTGC CTTGTAACAA CCACAGCCCC 
ACAACCCACC CAGAGGCAGG CGACGGCAGC CCAGTCCCCA GCTACGAGTA CCATGGGGAC 
GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240277.2
CDS	29..3217
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_011241975.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241975.3

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGGCAAGGAG AGGGAAGAAG GAAGGACTCT 
GCCCTTCTAA GTAAACGGCG GAAGTGTGGG AAATACGGTA TCACCAGCCT CCTGGCCAAT 
GGCGTATACT CAGCTGCATA CCCTCTGCAC GATGGGGACT ATGAGGGTGA CAACGTTGAG 
TTCAACGACA GGAAACTCCT GTATGAGGAA TGGGCAAGTT ACGGAGTCTT CTACAAATAC 
CAGCCCATTG ACCTGGTCAG GAAATACTTT GGTGAGAAGG TTGGCCTGTA CTTTGCCTGG 
CTTGGAGCCT ACACCCAGAT GCTCATCCCT GCCTCGATCG TGGGTGTCAT TGTCTTTCTC 
TATGGATGTG CCACTGTGGA CGAAAACATC CCCAGTATGG AGATGTGTGA CCAGAGATAC 
AACATCACCA TGTGTCCTCT GTGTGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC 
TGTGCCACAG CCCGTGCCAG TCACCTTTTT GATAACCCTG CCACCGTCTT CTTCTCTGTG 
TTTATGGCCC TCTGGGCTGC CACTTTCATG GAGCACTGGA AACGGAAGCA GATGAGGCTC 
AACTACCGAT GGGACCTCAC AGGCTTCGAG GAGGAGGAGG ATCATCCCAG AGCAGAGTAT 
GAAGCCAGAG TCTTAGAGAA GTCACTGAGA AAAGAATCCA GAAACAAAGA GACCGACAAG 
GTGAAGCTGA CCTGGAGGGA CCGATTCCCA GCCTATTTCA CCAATCTTGT CTCCATCATC 
TTCATGATCG CAGTGACATT TGCAATCGTC CTCGGAGTTA TCATCTATAG AATCTCCACA 
GCTGCAGCCT TGGCCATGAA CTCCTCCCCG TCTGTGCGGT CCAACATCCG GGTTACAGTC 
ACGGCCACCG CTGTTATCAT CAACCTCGTG GTCATCATTC TGCTGGATGA AGTTTACGGC 
TGCATTGCCA GGTGGCTCAC CAAGATTGAG GTCCCAAAGA CAGAGAAGAG CTTTGAGGAG 
AGGCTAACCT TCAAGGCCTT CCTGCTCAAG TTTGTGAACT CTTACACTCC CATCTTCTAT 
GTCGCCTTCT TCAAAGGCCG GTTTGTTGGT CGGCCCGGTG ACTACGTGTA CATCTTCCGC 
TCTTTCCGGA TGGAGGAGTG TGCCCCGGGC GGCTGCCTCA TGGAGCTCTG TATCCAGCTG 
AGCATCATTA TGCTGGGCAA GCAGCTAATC CAGAACAATC TCTTCGAGAT TGGCATCCCG 
AAGATGAAAA AGTTCATCCG CTACCTGAAG CTGCGCAGAC AGAGCCCCTC AGACCGTGAA 
GAGTACGTGA AGCGGAAGCA GCGCTATGAG GTGGACTTCA ACCTCGAACC TTTCGCCGGC 
CTCACGCCCG AGTACATGGA AATGATCATT CAGTTCGGCT TTGTCACCCT GTTTGTTGCG 
TCCTTCCCTC TGGCTCCACT CTTCGCCCTG CTAAACAACA TCATTGAGAT CCGCCTGGAT 
GCCAAAAAGT TTGTCACCGA GCTACGGAGG CCAGTAGCCA TCAGAGCCAA AGACATCGGC 
ATCTGGTATA ACATCCTCAG AGGTGTTGGG AAGCTGGCTG TCATCATTAA TGCCTTTGTG 
ATCTCCTTCA CGTCTGACTT CATCCCTCGC CTGGTGTACC TCTACATGTA CAGTCAGAAT 
GGGACCATGC ACGGCTTCGT CAATCACACG CTCTCTTCCT TCAATGTCAG CGACTTCCAG 
AATGGCACAG CACCCAATGA CCCACTGGAC CTGGGCTATG AGGTTCAGAT CTGCAGGTAT 
AAAGATTACC GGGAACCCCC ATGGTCAGAA CACAAGTATG ACATCTCCAA AGACTTCTGG 
GCTGTCCTGG CCGCCCGACT GGCATTTGTC ATTGTCTTCC AGAACCTGGT GATGTTCATG 
AGTGACTTTG TGGACTGGGT GATCCCTGAT ATCCCCAAAG ACATCAGCCA GCAGATCCAC 
AAAGAGAAGG TTCTCATGGT GGAGCTGTTT ATGCGTGAGG AGCAGGGCAA GCAGCAGCTA 
CTGGACACAT GGATGGAGAA GGAGAAGCCA AGGGATGTGC CTTGTAACAA CCACAGCCCC 
ACAACCCACC CAGAGGCAGG CGACGGCAGC CCAGTCCCCA GCTACGAGTA CCATGGGGAC 
GCGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241956.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097816.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu89316
Clone ID Related Accession (Same CDS sequence)	XM_030241956.1
Accession Version	XM_030241956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGAAGCTG TCAAGGATCA TCCCAGAGCA 
GAGTATGAAG CCAGAGTCTT AGAGAAGTCA CTGAGAAAAG AATCCAGAAA CAAAGAGACC 
GACAAGGTGA AGCTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTCCTCG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCGTCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACCGCTGT TATCATCAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CAAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCTTA CACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CCGGTGACTA CGTGTACATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCGGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATTATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATTGGC 
ATCCCGAAGA TGAAAAAGTT CATCCGCTAC CTGAAGCTGC GCAGACAGAG CCCCTCAGAC 
CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCTTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTTGT CACCCTGTTT 
GTTGCGTCCT TCCCTCTGGC TCCACTCTTC GCCCTGCTAA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGGCCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT CCTCAGAGGT GTTGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT 
CAGAATGGGA CCATGCACGG CTTCGTCAAT CACACGCTCT CTTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTATGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA ACCCCCATGG TCAGAACACA AGTATGACAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGACTGGCA TTTGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAAG AGAAGGTTCT CATGGTGGAG CTGTTTATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGCCCCACAA CCCACCCAGA GGCAGGCGAC GGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097816.1
CDS	27..3161
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241956.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241956.1

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGAAGCTG TCAAGGATCA TCCCAGAGCA 
GAGTATGAAG CCAGAGTCTT AGAGAAGTCA CTGAGAAAAG AATCCAGAAA CAAAGAGACC 
GACAAGGTGA AGCTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTCCTCG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCGTCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACCGCTGT TATCATCAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CAAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCTTA CACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CCGGTGACTA CGTGTACATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCGGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATTATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATTGGC 
ATCCCGAAGA TGAAAAAGTT CATCCGCTAC CTGAAGCTGC GCAGACAGAG CCCCTCAGAC 
CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCTTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTTGT CACCCTGTTT 
GTTGCGTCCT TCCCTCTGGC TCCACTCTTC GCCCTGCTAA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGGCCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT CCTCAGAGGT GTTGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT 
CAGAATGGGA CCATGCACGG CTTCGTCAAT CACACGCTCT CTTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTATGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA ACCCCCATGG TCAGAACACA AGTATGACAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGACTGGCA TTTGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAAG AGAAGGTTCT CATGGTGGAG CTGTTTATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGCCCCACAA CCCACCCAGA GGCAGGCGAC GGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241957.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097817.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu89317
Clone ID Related Accession (Same CDS sequence)	XM_030241957.1
Accession Version	XM_030241957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TATACTCAGC TGCATACCCT 
CTGCACGATG GGGACTATGA GGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGTTACGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGTG AGAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CGATCGTGGG TGTCATTGTC TTTCTCTATG GATGTGCCAC TGTGGACGAA 
AACATCCCCA GTATGGAGAT GTGTGACCAG AGATACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCCCG TGCCAGTCAC 
CTTTTTGATA ACCCTGCCAC CGTCTTCTTC TCTGTGTTTA TGGCCCTCTG GGCTGCCACT 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGGCTCAACT ACCGATGGGA CCTCACAGGC 
TTCGAGGAGG AGGAGGAAGC TGTCAAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA AGTGCCGAAA TGTTCCAGAA 
GAACCGACCA ACAAATGGAA GCAGAGGGTT AGGACAGCCA TGGCAGGGGT GAAATTGACC 
GACAAGGTGA AGCTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTCCTCG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCGTCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACCGCTGT TATCATCAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CAAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCTTA CACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CCGGTGACTA CGTGTACATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCGGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATTATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATTGGC 
ATCCCGAAGA TGAAAAAGTT CATCCGCTAC CTGAAGCTGC GCAGACAGAG CCCCTCAGAC 
CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCTTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTTGT CACCCTGTTT 
GTTGCGTCCT TCCCTCTGGC TCCACTCTTC GCCCTGCTAA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGGCCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT CCTCAGAGGT GTTGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT 
CAGAATGGGA CCATGCACGG CTTCGTCAAT CACACGCTCT CTTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTATGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA ACCCCCATGG TCAGAACACA AGTATGACAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGACTGGCA TTTGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAAG AGAAGGTTCT CATGGTGGAG CTGTTTATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGCCCCACAA CCCACCCAGA GGCAGGCGAC GGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097817.1
CDS	143..3277
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241957.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241957.1

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGTT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCGGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTGAA CTCTCTGTCC 
GTGGACCCCG ACGCCGAATG CAAGTATGGA CTATACTTCA GGGATGGCAA ACGGAAGGTG 
GACTACATCT TGGTATACCA TCACAAGAGA GCCTCAGGGA GCAGGACTCT GGCCAGGAGG 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGCGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GCCCTGTGGA TGCAGGCTCA CCGGAAGTCC CCATGGATTA CCATGAAGAT 
GACAAACGCT TCAGACGGGA GGAATATGAG GGCAACCTGT TGGAGGCAGG CCTGGAGTTG 
GAGAATGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GTAGGGAAGC TGAGTTTTTG AAACTAAAGA TGCCCACAAA GAAGGTGTAC 
CACATCAGTG AGACGCGAGG CCTCCTGAAA ACCATCAACT CGGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGGCCACAGA CCACAAAGAG GCTCTCCTAT 
CCCTTCTCCC GGGAGAAGCA ACACCTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TATACTCAGC TGCATACCCT 
CTGCACGATG GGGACTATGA GGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGTTACGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGTG AGAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CGATCGTGGG TGTCATTGTC TTTCTCTATG GATGTGCCAC TGTGGACGAA 
AACATCCCCA GTATGGAGAT GTGTGACCAG AGATACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCCCG TGCCAGTCAC 
CTTTTTGATA ACCCTGCCAC CGTCTTCTTC TCTGTGTTTA TGGCCCTCTG GGCTGCCACT 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGGCTCAACT ACCGATGGGA CCTCACAGGC 
TTCGAGGAGG AGGAGGAAGC TGTCAAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA AGTGCCGAAA TGTTCCAGAA 
GAACCGACCA ACAAATGGAA GCAGAGGGTT AGGACAGCCA TGGCAGGGGT GAAATTGACC 
GACAAGGTGA AGCTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTCCTCG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCGTCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACCGCTGT TATCATCAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CAAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCTTA CACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CCGGTGACTA CGTGTACATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCGGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATTATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATTGGC 
ATCCCGAAGA TGAAAAAGTT CATCCGCTAC CTGAAGCTGC GCAGACAGAG CCCCTCAGAC 
CGTGAAGAGT ACGTGAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCTTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTTGT CACCCTGTTT 
GTTGCGTCCT TCCCTCTGGC TCCACTCTTC GCCCTGCTAA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGGCCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT CCTCAGAGGT GTTGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT 
CAGAATGGGA CCATGCACGG CTTCGTCAAT CACACGCTCT CTTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTATGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA ACCCCCATGG TCAGAACACA AGTATGACAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGACTGGCA TTTGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAAG AGAAGGTTCT CATGGTGGAG CTGTTTATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGCCCCACAA CCCACCCAGA GGCAGGCGAC GGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGACGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241958.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097818.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu89318
Clone ID Related Accession (Same CDS sequence)	XM_030241958.1
Accession Version	XM_030241958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GTATCACCAG CCTCCTGGCC AATGGCGTAT ACTCAGCTGC ATACCCTCTG 
CACGATGGGG ACTATGAGGG TGACAACGTT GAGTTCAACG ACAGGAAACT CCTGTATGAG 
GAATGGGCAA GTTACGGAGT CTTCTACAAA TACCAGCCCA TTGACCTGGT CAGGAAATAC 
TTTGGTGAGA AGGTTGGCCT GTACTTTGCC TGGCTTGGAG CCTACACCCA GATGCTCATC 
CCTGCCTCGA TCGTGGGTGT CATTGTCTTT CTCTATGGAT GTGCCACTGT GGACGAAAAC 
ATCCCCAGTA TGGAGATGTG TGACCAGAGA TACAACATCA CCATGTGTCC TCTGTGTGAC 
AAGACCTGCA GCTACTGGAA GATGAGCTCA GCCTGTGCCA CAGCCCGTGC CAGTCACCTT 
TTTGATAACC CTGCCACCGT CTTCTTCTCT GTGTTTATGG CCCTCTGGGC TGCCACTTTC 
ATGGAGCACT GGAAACGGAA GCAGATGAGG CTCAACTACC GATGGGACCT CACAGGCTTC 
GAGGAGGAGG AGGAAGCTGT CAAGGATCAT CCCAGAGCAG AGTATGAAGC CAGAGTCTTA 
GAGAAGTCAC TGAGAAAAGA ATCCAGAAAC AAAGAGAAGT GCCGAAATGT TCCAGAAGAA 
CCGACCAACA AATGGAAGCA GAGGGTTAGG ACAGCCATGG CAGGGGTGAA ATTGACCGAC 
AAGGTGAAGC TGACCTGGAG GGACCGATTC CCAGCCTATT TCACCAATCT TGTCTCCATC 
ATCTTCATGA TCGCAGTGAC ATTTGCAATC GTCCTCGGAG TTATCATCTA TAGAATCTCC 
ACAGCTGCAG CCTTGGCCAT GAACTCCTCC CCGTCTGTGC GGTCCAACAT CCGGGTTACA 
GTCACGGCCA CCGCTGTTAT CATCAACCTC GTGGTCATCA TTCTGCTGGA TGAAGTTTAC 
GGCTGCATTG CCAGGTGGCT CACCAAGATT GAGGTCCCAA AGACAGAGAA GAGCTTTGAG 
GAGAGGCTAA CCTTCAAGGC CTTCCTGCTC AAGTTTGTGA ACTCTTACAC TCCCATCTTC 
TATGTCGCCT TCTTCAAAGG CCGGTTTGTT GGTCGGCCCG GTGACTACGT GTACATCTTC 
CGCTCTTTCC GGATGGAGGA GTGTGCCCCG GGCGGCTGCC TCATGGAGCT CTGTATCCAG 
CTGAGCATCA TTATGCTGGG CAAGCAGCTA ATCCAGAACA ATCTCTTCGA GATTGGCATC 
CCGAAGATGA AAAAGTTCAT CCGCTACCTG AAGCTGCGCA GACAGAGCCC CTCAGACCGT 
GAAGAGTACG TGAAGCGGAA GCAGCGCTAT GAGGTGGACT TCAACCTCGA ACCTTTCGCC 
GGCCTCACGC CCGAGTACAT GGAAATGATC ATTCAGTTCG GCTTTGTCAC CCTGTTTGTT 
GCGTCCTTCC CTCTGGCTCC ACTCTTCGCC CTGCTAAACA ACATCATTGA GATCCGCCTG 
GATGCCAAAA AGTTTGTCAC CGAGCTACGG AGGCCAGTAG CCATCAGAGC CAAAGACATC 
GGCATCTGGT ATAACATCCT CAGAGGTGTT GGGAAGCTGG CTGTCATCAT TAATGCCTTT 
GTGATCTCCT TCACGTCTGA CTTCATCCCT CGCCTGGTGT ACCTCTACAT GTACAGTCAG 
AATGGGACCA TGCACGGCTT CGTCAATCAC ACGCTCTCTT CCTTCAATGT CAGCGACTTC 
CAGAATGGCA CAGCACCCAA TGACCCACTG GACCTGGGCT ATGAGGTTCA GATCTGCAGG 
TATAAAGATT ACCGGGAACC CCCATGGTCA GAACACAAGT ATGACATCTC CAAAGACTTC 
TGGGCTGTCC TGGCCGCCCG ACTGGCATTT GTCATTGTCT TCCAGAACCT GGTGATGTTC 
ATGAGTGACT TTGTGGACTG GGTGATCCCT GATATCCCCA AAGACATCAG CCAGCAGATC 
CACAAAGAGA AGGTTCTCAT GGTGGAGCTG TTTATGCGTG AGGAGCAGGG CAAGCAGCAG 
CTACTGGACA CATGGATGGA GAAGGAGAAG CCAAGGGATG TGCCTTGTAA CAACCACAGC 
CCCACAACCC ACCCAGAGGC AGGCGACGGC AGCCCAGTCC CCAGCTACGA GTACCATGGG 
GACGCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097818.1
CDS	143..3274
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241958.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241958.1

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GTATCACCAG CCTCCTGGCC AATGGCGTAT ACTCAGCTGC ATACCCTCTG 
CACGATGGGG ACTATGAGGG TGACAACGTT GAGTTCAACG ACAGGAAACT CCTGTATGAG 
GAATGGGCAA GTTACGGAGT CTTCTACAAA TACCAGCCCA TTGACCTGGT CAGGAAATAC 
TTTGGTGAGA AGGTTGGCCT GTACTTTGCC TGGCTTGGAG CCTACACCCA GATGCTCATC 
CCTGCCTCGA TCGTGGGTGT CATTGTCTTT CTCTATGGAT GTGCCACTGT GGACGAAAAC 
ATCCCCAGTA TGGAGATGTG TGACCAGAGA TACAACATCA CCATGTGTCC TCTGTGTGAC 
AAGACCTGCA GCTACTGGAA GATGAGCTCA GCCTGTGCCA CAGCCCGTGC CAGTCACCTT 
TTTGATAACC CTGCCACCGT CTTCTTCTCT GTGTTTATGG CCCTCTGGGC TGCCACTTTC 
ATGGAGCACT GGAAACGGAA GCAGATGAGG CTCAACTACC GATGGGACCT CACAGGCTTC 
GAGGAGGAGG AGGAAGCTGT CAAGGATCAT CCCAGAGCAG AGTATGAAGC CAGAGTCTTA 
GAGAAGTCAC TGAGAAAAGA ATCCAGAAAC AAAGAGAAGT GCCGAAATGT TCCAGAAGAA 
CCGACCAACA AATGGAAGCA GAGGGTTAGG ACAGCCATGG CAGGGGTGAA ATTGACCGAC 
AAGGTGAAGC TGACCTGGAG GGACCGATTC CCAGCCTATT TCACCAATCT TGTCTCCATC 
ATCTTCATGA TCGCAGTGAC ATTTGCAATC GTCCTCGGAG TTATCATCTA TAGAATCTCC 
ACAGCTGCAG CCTTGGCCAT GAACTCCTCC CCGTCTGTGC GGTCCAACAT CCGGGTTACA 
GTCACGGCCA CCGCTGTTAT CATCAACCTC GTGGTCATCA TTCTGCTGGA TGAAGTTTAC 
GGCTGCATTG CCAGGTGGCT CACCAAGATT GAGGTCCCAA AGACAGAGAA GAGCTTTGAG 
GAGAGGCTAA CCTTCAAGGC CTTCCTGCTC AAGTTTGTGA ACTCTTACAC TCCCATCTTC 
TATGTCGCCT TCTTCAAAGG CCGGTTTGTT GGTCGGCCCG GTGACTACGT GTACATCTTC 
CGCTCTTTCC GGATGGAGGA GTGTGCCCCG GGCGGCTGCC TCATGGAGCT CTGTATCCAG 
CTGAGCATCA TTATGCTGGG CAAGCAGCTA ATCCAGAACA ATCTCTTCGA GATTGGCATC 
CCGAAGATGA AAAAGTTCAT CCGCTACCTG AAGCTGCGCA GACAGAGCCC CTCAGACCGT 
GAAGAGTACG TGAAGCGGAA GCAGCGCTAT GAGGTGGACT TCAACCTCGA ACCTTTCGCC 
GGCCTCACGC CCGAGTACAT GGAAATGATC ATTCAGTTCG GCTTTGTCAC CCTGTTTGTT 
GCGTCCTTCC CTCTGGCTCC ACTCTTCGCC CTGCTAAACA ACATCATTGA GATCCGCCTG 
GATGCCAAAA AGTTTGTCAC CGAGCTACGG AGGCCAGTAG CCATCAGAGC CAAAGACATC 
GGCATCTGGT ATAACATCCT CAGAGGTGTT GGGAAGCTGG CTGTCATCAT TAATGCCTTT 
GTGATCTCCT TCACGTCTGA CTTCATCCCT CGCCTGGTGT ACCTCTACAT GTACAGTCAG 
AATGGGACCA TGCACGGCTT CGTCAATCAC ACGCTCTCTT CCTTCAATGT CAGCGACTTC 
CAGAATGGCA CAGCACCCAA TGACCCACTG GACCTGGGCT ATGAGGTTCA GATCTGCAGG 
TATAAAGATT ACCGGGAACC CCCATGGTCA GAACACAAGT ATGACATCTC CAAAGACTTC 
TGGGCTGTCC TGGCCGCCCG ACTGGCATTT GTCATTGTCT TCCAGAACCT GGTGATGTTC 
ATGAGTGACT TTGTGGACTG GGTGATCCCT GATATCCCCA AAGACATCAG CCAGCAGATC 
CACAAAGAGA AGGTTCTCAT GGTGGAGCTG TTTATGCGTG AGGAGCAGGG CAAGCAGCAG 
CTACTGGACA CATGGATGGA GAAGGAGAAG CCAAGGGATG TGCCTTGTAA CAACCACAGC 
CCCACAACCC ACCCAGAGGC AGGCGACGGC AGCCCAGTCC CCAGCTACGA GTACCATGGG 
GACGCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241959.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097819.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu89319
Clone ID Related Accession (Same CDS sequence)	XM_030241959.1
Accession Version	XM_030241959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097819.1
CDS	28..3150
Translation	MGLGCDAWKQQPKAHRSSRSWVWQAEPARGQVHGARAPSGRARWRQRPGGREGGGSGHVPSAPGQHRFSVADATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241959.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241959.1

ORF Insert Sequence:

ATGGGACTTG GCTGTGATGC CTGGAAGCAA CAACCCAAGG CTCACAGGAG TTCCCGAAGC 
TGGGTTTGGC AGGCGGAGCC GGCGCGCGGC CAAGTTCACG GCGCGCGGGC GCCCTCGGGA 
CGGGCCAGGT GGCGGCAGCG CCCGGGAGGT CGCGAGGGAG GAGGGAGCGG CCACGTCCCG 
TCCGCGCCAG GCCAGCACCG CTTCTCCGTT GCAGACGCCA CCGGGGTTGT GGATGGGGAG 
CGCGAGGCCA CCATGAGGGT CCCCGAGAAG TACTCGACGC TCCCGGCGGA GGACCGCAGC 
GTCCACATCG TGAACATCTG CGCCATCGAG GACCTGGGCT ACCTGCCGTC CGAGGGCACG 
TTGCTGAACT CTCTGTCCGT GGACCCCGAC GCCGAATGCA AGTATGGACT ATACTTCAGG 
GATGGCAAAC GGAAGGTGGA CTACATCTTG GTATACCATC ACAAGAGAGC CTCAGGGAGC 
AGGACTCTGG CCAGGAGGGG ACTACAAAAT GACATGGTCC TGGGGACCCG CAGCGTCAGG 
CAGGACCAGC CCCTTCCCGG GAAGGGGAGC CCTGTGGATG CAGGCTCACC GGAAGTCCCC 
ATGGATTACC ATGAAGATGA CAAACGCTTC AGACGGGAGG AATATGAGGG CAACCTGTTG 
GAGGCAGGCC TGGAGTTGGA GAATGACGAG GATACCAAAA TCCATGGTGT CGGGTTTGTG 
AAGATCCATG CGCCCTGGCA TGTGCTCTGT AGGGAAGCTG AGTTTTTGAA ACTAAAGATG 
CCCACAAAGA AGGTGTACCA CATCAGTGAG ACGCGAGGCC TCCTGAAAAC CATCAACTCG 
GTTCTGCAGA AGATCACAGA CCCCATCCAG CCCAAGGTGG CTGAGCACAG GCCACAGACC 
ACAAAGAGGC TCTCCTATCC CTTCTCCCGG GAGAAGCAAC ACCTATTCGA CCTGACTGAC 
AGGGACTCTT TTTTCGACAG CAAAACCCGG AGCACAATAG TCTATGAAAT CCTGAAGAGA 
ACAACGTGCA CCAAGGCCAA GTACAGCATG GGTATCACCA GCCTCCTGGC CAATGGCGTA 
TACTCAGCTG CATACCCTCT GCACGATGGG GACTATGAGG GTGACAACGT TGAGTTCAAC 
GACAGGAAAC TCCTGTATGA GGAATGGGCA AGTTACGGAG TCTTCTACAA ATACCAGCCC 
ATTGACCTGG TCAGGAAATA CTTTGGTGAG AAGGTTGGCC TGTACTTTGC CTGGCTTGGA 
GCCTACACCC AGATGCTCAT CCCTGCCTCG ATCGTGGGTG TCATTGTCTT TCTCTATGGA 
TGTGCCACTG TGGACGAAAA CATCCCCAGT ATGGAGATGT GTGACCAGAG ATACAACATC 
ACCATGTGTC CTCTGTGTGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGTGCC 
ACAGCCCGTG CCAGTCACCT TTTTGATAAC CCTGCCACCG TCTTCTTCTC TGTGTTTATG 
GCCCTCTGGG CTGCCACTTT CATGGAGCAC TGGAAACGGA AGCAGATGAG GCTCAACTAC 
CGATGGGACC TCACAGGCTT CGAGGAGGAG GAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG 
CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG 
AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC 
GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC 
CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC 
ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG 
AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC 
CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_030241960.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097820.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu89320
Clone ID Related Accession (Same CDS sequence)	XM_030241960.1
Accession Version	XM_030241960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTATACTC AGCTGCATAC 
CCTCTGCACG ATGGGGACTA TGAGGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGTTA CGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GTGAGAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCGATCGT GGGTGTCATT GTCTTTCTCT ATGGATGTGC CACTGTGGAC 
GAAAACATCC CCAGTATGGA GATGTGTGAC CAGAGATACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC CCGTGCCAGT 
CACCTTTTTG ATAACCCTGC CACCGTCTTC TTCTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACTTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGGCTCA ACTACCGATG GGACCTCACA 
GGCTTCGAGG AGGAGGAGGA TCATCCCAGA GCAGAGTATG AAGCCAGAGT CTTAGAGAAG 
TCACTGAGAA AAGAATCCAG AAACAAAGAG ACCGACAAGG TGAAGCTGAC CTGGAGGGAC 
CGATTCCCAG CCTATTTCAC CAATCTTGTC TCCATCATCT TCATGATCGC AGTGACATTT 
GCAATCGTCC TCGGAGTTAT CATCTATAGA ATCTCCACAG CTGCAGCCTT GGCCATGAAC 
TCCTCCCCGT CTGTGCGGTC CAACATCCGG GTTACAGTCA CGGCCACCGC TGTTATCATC 
AACCTCGTGG TCATCATTCT GCTGGATGAA GTTTACGGCT GCATTGCCAG GTGGCTCACC 
AAGATTGAGG TCCCAAAGAC AGAGAAGAGC TTTGAGGAGA GGCTAACCTT CAAGGCCTTC 
CTGCTCAAGT TTGTGAACTC TTACACTCCC ATCTTCTATG TCGCCTTCTT CAAAGGCCGG 
TTTGTTGGTC GGCCCGGTGA CTACGTGTAC ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT 
GCCCCGGGCG GCTGCCTCAT GGAGCTCTGT ATCCAGCTGA GCATCATTAT GCTGGGCAAG 
CAGCTAATCC AGAACAATCT CTTCGAGATT GGCATCCCGA AGATGAAAAA GTTCATCCGC 
TACCTGAAGC TGCGCAGACA GAGCCCCTCA GACCGTGAAG AGTACGTGAA GCGGAAGCAG 
CGCTATGAGG TGGACTTCAA CCTCGAACCT TTCGCCGGCC TCACGCCCGA GTACATGGAA 
ATGATCATTC AGTTCGGCTT TGTCACCCTG TTTGTTGCGT CCTTCCCTCT GGCTCCACTC 
TTCGCCCTGC TAAACAACAT CATTGAGATC CGCCTGGATG CCAAAAAGTT TGTCACCGAG 
CTACGGAGGC CAGTAGCCAT CAGAGCCAAA GACATCGGCA TCTGGTATAA CATCCTCAGA 
GGTGTTGGGA AGCTGGCTGT CATCATTAAT GCCTTTGTGA TCTCCTTCAC GTCTGACTTC 
ATCCCTCGCC TGGTGTACCT CTACATGTAC AGTCAGAATG GGACCATGCA CGGCTTCGTC 
AATCACACGC TCTCTTCCTT CAATGTCAGC GACTTCCAGA ATGGCACAGC ACCCAATGAC 
CCACTGGACC TGGGCTATGA GGTTCAGATC TGCAGGTATA AAGATTACCG GGAACCCCCA 
TGGTCAGAAC ACAAGTATGA CATCTCCAAA GACTTCTGGG CTGTCCTGGC CGCCCGACTG 
GCATTTGTCA TTGTCTTCCA GAACCTGGTG ATGTTCATGA GTGACTTTGT GGACTGGGTG 
ATCCCTGATA TCCCCAAAGA CATCAGCCAG CAGATCCACA AAGAGAAGGT TCTCATGGTG 
GAGCTGTTTA TGCGTGAGGA GCAGGGCAAG CAGCAGCTAC TGGACACATG GATGGAGAAG 
GAGAAGCCAA GGGATGTGCC TTGTAACAAC CACAGCCCCA CAACCCACCC AGAGGCAGGC 
GACGGCAGCC CAGTCCCCAG CTACGAGTAC CATGGGGACG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097820.1
CDS	143..3250
Translation	MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSDATGVVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_030241960.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241960.1

ORF Insert Sequence:

ATGCAGGACG CGCAGGACAG CGACATCGGC CTGGAGGGCT TGACCCCAGA GGGAACCTCT 
GCGGACCGAG AGTGCCAGCG GGGACCGGAA ACAATCGCGC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGTTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCGGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTGAACTC TCTGTCCGTG 
GACCCCGACG CCGAATGCAA GTATGGACTA TACTTCAGGG ATGGCAAACG GAAGGTGGAC 
TACATCTTGG TATACCATCA CAAGAGAGCC TCAGGGAGCA GGACTCTGGC CAGGAGGGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGCGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGCC CTGTGGATGC AGGCTCACCG GAAGTCCCCA TGGATTACCA TGAAGATGAC 
AAACGCTTCA GACGGGAGGA ATATGAGGGC AACCTGTTGG AGGCAGGCCT GGAGTTGGAG 
AATGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGTA GGGAAGCTGA GTTTTTGAAA CTAAAGATGC CCACAAAGAA GGTGTACCAC 
ATCAGTGAGA CGCGAGGCCT CCTGAAAACC ATCAACTCGG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGG CCACAGACCA CAAAGAGGCT CTCCTATCCC 
TTCTCCCGGG AGAAGCAACA CCTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTATACTC AGCTGCATAC 
CCTCTGCACG ATGGGGACTA TGAGGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGTTA CGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GTGAGAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCGATCGT GGGTGTCATT GTCTTTCTCT ATGGATGTGC CACTGTGGAC 
GAAAACATCC CCAGTATGGA GATGTGTGAC CAGAGATACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC CCGTGCCAGT 
CACCTTTTTG ATAACCCTGC CACCGTCTTC TTCTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACTTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGGCTCA ACTACCGATG GGACCTCACA 
GGCTTCGAGG AGGAGGAGGA TCATCCCAGA GCAGAGTATG AAGCCAGAGT CTTAGAGAAG 
TCACTGAGAA AAGAATCCAG AAACAAAGAG ACCGACAAGG TGAAGCTGAC CTGGAGGGAC 
CGATTCCCAG CCTATTTCAC CAATCTTGTC TCCATCATCT TCATGATCGC AGTGACATTT 
GCAATCGTCC TCGGAGTTAT CATCTATAGA ATCTCCACAG CTGCAGCCTT GGCCATGAAC 
TCCTCCCCGT CTGTGCGGTC CAACATCCGG GTTACAGTCA CGGCCACCGC TGTTATCATC 
AACCTCGTGG TCATCATTCT GCTGGATGAA GTTTACGGCT GCATTGCCAG GTGGCTCACC 
AAGATTGAGG TCCCAAAGAC AGAGAAGAGC TTTGAGGAGA GGCTAACCTT CAAGGCCTTC 
CTGCTCAAGT TTGTGAACTC TTACACTCCC ATCTTCTATG TCGCCTTCTT CAAAGGCCGG 
TTTGTTGGTC GGCCCGGTGA CTACGTGTAC ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT 
GCCCCGGGCG GCTGCCTCAT GGAGCTCTGT ATCCAGCTGA GCATCATTAT GCTGGGCAAG 
CAGCTAATCC AGAACAATCT CTTCGAGATT GGCATCCCGA AGATGAAAAA GTTCATCCGC 
TACCTGAAGC TGCGCAGACA GAGCCCCTCA GACCGTGAAG AGTACGTGAA GCGGAAGCAG 
CGCTATGAGG TGGACTTCAA CCTCGAACCT TTCGCCGGCC TCACGCCCGA GTACATGGAA 
ATGATCATTC AGTTCGGCTT TGTCACCCTG TTTGTTGCGT CCTTCCCTCT GGCTCCACTC 
TTCGCCCTGC TAAACAACAT CATTGAGATC CGCCTGGATG CCAAAAAGTT TGTCACCGAG 
CTACGGAGGC CAGTAGCCAT CAGAGCCAAA GACATCGGCA TCTGGTATAA CATCCTCAGA 
GGTGTTGGGA AGCTGGCTGT CATCATTAAT GCCTTTGTGA TCTCCTTCAC GTCTGACTTC 
ATCCCTCGCC TGGTGTACCT CTACATGTAC AGTCAGAATG GGACCATGCA CGGCTTCGTC 
AATCACACGC TCTCTTCCTT CAATGTCAGC GACTTCCAGA ATGGCACAGC ACCCAATGAC 
CCACTGGACC TGGGCTATGA GGTTCAGATC TGCAGGTATA AAGATTACCG GGAACCCCCA 
TGGTCAGAAC ACAAGTATGA CATCTCCAAA GACTTCTGGG CTGTCCTGGC CGCCCGACTG 
GCATTTGTCA TTGTCTTCCA GAACCTGGTG ATGTTCATGA GTGACTTTGT GGACTGGGTG 
ATCCCTGATA TCCCCAAAGA CATCAGCCAG CAGATCCACA AAGAGAAGGT TCTCATGGTG 
GAGCTGTTTA TGCGTGAGGA GCAGGGCAAG CAGCAGCTAC TGGACACATG GATGGAGAAG 
GAGAAGCCAA GGGATGTGCC TTGTAACAAC CACAGCCCCA CAACCCACCC AGAGGCAGGC 
GACGGCAGCC CAGTCCCCAG CTACGAGTAC CATGGGGACG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_011241974.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240276.1 Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X2, mRNA.

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CloneID	OMu72036
Clone ID Related Accession (Same CDS sequence)	XM_011241974.2
Accession Version	XM_011241974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	anoctamin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240276.1
CDS	179..3139
Translation	MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKCRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

Target ORF information:

RefSeq Version	XM_011241974.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anoctamin 1, calcium activated chloride channel (Ano1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241974.2

ORF Insert Sequence:

ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG 
AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT 
CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG 
AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC 
AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC 
CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT 
GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG 
GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG 
CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG 
GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG 
ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC 
TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT 
TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC 
AAGGCCAAGT ACAGCATGGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA 
TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG 
GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC 
AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT 
GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC 
ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGTG CCGAAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACCGACA AGGTGAAGCT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TCCTCGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CGTCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC CGCTGTTATC ATCAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCAAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCTTACACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCCGG TGACTACGTG 
TACATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCGG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT TATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATTGGCATCC CGAAGATGAA AAAGTTCATC CGCTACCTGA AGCTGCGCAG ACAGAGCCCC 
TCAGACCGTG AAGAGTACGT GAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCTTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTTGTCACC 
CTGTTTGTTG CGTCCTTCCC TCTGGCTCCA CTCTTCGCCC TGCTAAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GGCCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATCCTC AGAGGTGTTG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG 
TACAGTCAGA ATGGGACCAT GCACGGCTTC GTCAATCACA CGCTCTCTTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA TGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAACCC CCATGGTCAG AACACAAGTA TGACATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGA CTGGCATTTG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAAGAGAA GGTTCTCATG GTGGAGCTGT TTATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGCC CCACAACCCA CCCAGAGGCA GGCGACGGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG ACGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain.
Proceedings of the National Academy of Sciences of the United States of America116(25)12494-12499(2019 Jun)
Hong GS,Lee SH,Lee B,Choi JH,Oh SJ,Jang Y,Hwang EM,Kim H,Jung J,Kim IB,Oh U

Nephron-specific knockout of TMEM16A leads to reduced number of glomeruli and albuminuria.
American journal of physiology. Renal physiology315(6)F1777-F1786(2018 12)
Schenk LK,Buchholz B,Henke SF,Michgehl U,Daniel C,Amann K,Kunzelmann K,Pavenstädt H

Calcium-dependent electrostatic control of anion access to the pore of the calcium-activated chloride channel TMEM16A.
eLife7(2018 10)
Lam AK,Dutzler R

Transmembrane member 16A participates in hydrogen peroxide-induced apoptosis by facilitating mitochondria-dependent pathway in vascular smooth muscle cells.
British journal of pharmacology175(18)3669-3684(2018 09)
Zeng JW,Chen BY,Lv XF,Sun L,Zeng XL,Zheng HQ,Du YH,Wang GL,Ma MM,Guan YY

Tissue-specific variation in 5'-terminal exons of mouse Anoctamin 1 transcript induces N-terminal variation of its protein via alternative translational start sites.
Biochemical and biophysical research communications503(3)1710-1715(2018 09)
Kamikawa A,Sakazaki J,Ichii O

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Ano1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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