NNT cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	NNT
Entrez Gene ID	100012732
Full Name	nicotinamide nucleotide transhydrogenase
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001364074.4	XP_001364111.1	NAD(P) transhydrogenase, mitochondrial
XM_007487387.2	XP_007487449.1	NAD(P) transhydrogenase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following NNT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NNT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMc15898	XM_007487387.2 Latest version!	Monodelphis domestica nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMc15898	XM_001364074.4 Latest version!	Monodelphis domestica nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NNT ( XM_001364074.4 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001364111.1 Monodelphis domestica nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc15898
Clone ID Related Accession (Same CDS sequence)	XM_001364074.4 , XM_007487387.2
Accession Version	XM_001364074.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NAD(P) transhydrogenase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001364074.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA TATTGAAAAC AGTGGTGACA AGCTACTCAA GTCCTCTACT CATTAATTTG 
GGATCCTTTA AGGTGCTACC TGGGAAGACA GAAGTTTTAC GAACTTTTCA TACCAATCAG 
ACACTGTGGT GTAAAGCACC TGTAAAACCA GGAATTCCAT ATAAACAATT GACTGTGGGA 
GTACCCAAGG AGATTTTCCA AAATGAGAAG AGAGTGGCTT TGTCACCTGC TGGTGTTCAG 
ACCTTGGTTA AACAGGGTTT TAATGTGGTG GTGGAATCTG GTGCAGGTGA AGCCTCTAGG 
TTTTCTGATG ATCACTACAA AGCTGCAGGA GCTCTAATCC AAGGGACTCG GGAAGTATTA 
GCATCTGATT TAGTTGTCAA AGTTAGAGCT CCCATGCTAA ACGTTGCATT AGGCACCCAT 
GAGGCTGATC TTTTAAAGAA ATCTGGCACC CTGATTAGTT TTATCTACCC AGCTCAAAAT 
CCAGATTTGC TGAGCAAACT TTCTGAAAGA AAAACTACTG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CCATTGCTCA GGGATATGAT GCTCTTAGCT CAATGGCCAA CATTGCAGGT 
TACAAGGCTG TTGTCCTAGC GGCAAATCAT TTTGGTCGTT TTTTCACTGG CCAGATCACA 
GCAGCTGGAA AAGTTCCGCC AGCTAAGATT CTTATCATTG GTGGTGGAGT CGCTGGGCTT 
GCTTCTGCTG GAGCAGCTAA GGCTATGGGG GCAATTGTTC GAGGATTTGA TACCAGAGCT 
GCAGCTTTGG AGCAGTTTAA GTCTCTTGGG GCAGAGCCTT TGGAAGTAGA CTTGAAAGAG 
TCAGGAGAAG GACAAGGAGG GTATGCCAAA GAAATGTCCA AAGAGTTCAT TGAAGCAGAA 
ATGAAACTAT TTGCCCAGCA ATGTAAAGAA GTTGATATTC TCATTAGTAC CGCCCTTATT 
CCTGGTAAAA AAGCTCCGAT TTTGTTTACA AAAGATATGA TTGAATCAAT GAAAGAAGGT 
TCAGTTGTTG TGGATTTAGC AGCTGAAGCA GGAGGAAACT TTGAAACTAC TAAGCCTGGA 
GAACTTTATA TCCATAAGGG AATTACTCAC ATAGGATACA CAGACCTTCC TAGTCGAATG 
GCTACCCAGG CCAGTTCACT GTATTCGAAC AATATTATCA AACTTCTGAA GGCAATTAGT 
CCTGATAAAG ATAATTTCCA TTTTGAGGTG AAGGATGACT TTGATTTTGG CACAATGGGT 
CATGTCATTA GGGGGACCGT GGTAATGAAG GATGGCAAGA ACATTTTCCC AGCACCAACA 
CCAAAAAATA TTCCTCAGGG AGCCCCTGTA AAACCAAAGA CAGTAGCTGA GCTTGAAGCT 
GAGAAAGCAG CTACTCTTAC CCCATTCCAC AAGACCATGA CCACAGCAAC TGCATATACA 
GCAGGTCTAA CTAGTATGCT TGGTTTGGGC ATCATAGCCC CCAATGCAGC CTTTTCTCAG 
ATGGTGACCA CTTTTGGATT AGCTGGCATT GTGGGCTACC ACACAGTGTG GGGTGTCACT 
CCTGCTCTCC ACTCACCACT GATGTCTGTG ACTAATGCTA TCTCAGGTTT GACTGCAGTT 
GGAGGGTTGG CATTAATGGG AGGACACATC TATCCTTCCA ATACTCCTCA GGGTCTCGCT 
ACGCTTGCTA CTTTCATATC CTCTGTCAAC ATTGCAGGTG GTTTCCTGGT GACCCAGAGG 
ATGCTGGACA TGTTCAAACG TCCTACTGAC CCCCCTGAAT ACAATTACTT GTATCTGCTC 
CCAGGTGGTA CCTTTGTTGG GGGTTATTTA GCTTCACTTT ACAGTGGCTA TGATATTGAA 
CAGATGATAT ATCTGGGCTC AGGCCTATGT TGTGTTGGTG CCCTGGCTGG TCTTTCCACA 
CAAGGAACTG CTCGTCTTGG GAATGCCTTG GGCATGATTG GTGTTGCTGG AGGTTTGGCA 
GCTACTCTGG GAAGTCTTAA TCCAAATCCA GAATTGTTAG CTCAGATGTC TGGGGCAATG 
ACTCTGGGTG GAACCATTGG TTTGATGATT GCTAAGCGTA TCCAGATTAC TGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTGGTA GGTTTGGCGG CTGTGTTAAC CTGTGTGGCA 
GAATATATGG TTGAATATCC ACATTTTGCT GTGGATCCAG CAGCAAACCT TACGAAGATT 
GTGGCTTACC TGGGTACTTA TATTGGTGGT GTGACTTTTA GTGGCTCCCT CATTGCCTAT 
GGAAAATTGC AGGGTATATT GAAATCGGCC CCTCTCCTTC TACCTGGAAG GCATTTACTC 
AATTTAGGCT TATTGACTGC TAGTGCAGGT GGATTAATTC CCTACTTGCT TGATCCCAGC 
TACACAATGG GAATTACCTG TCTAGGTTCT GTATCTGCTC TCTCAGCTAT CATGGGTGTG 
ACTTTAACAG CTGCTATTGG TGGTGCTGAC ATGCCCGTAG TCATCACTGT ACTGAATAGC 
TACTCAGGGT GGGCTCTGTG TGCTGAAGGT TTCCTTCTCA ACAACAACTT GCTTACTATT 
GTGGGTGCTC TGATTGGCTC CTCTGGTGCT ATCCTCTCCT ACATCATGTG TGTGGCTATG 
AATCGTTCCC TGGCTAATGT GATCCTTGGA GGCTATGGCA CTACTTCAAC CGCTGGTGGC 
AAACCTATGG AAATTTCTGG CTCACATACT GAAATTAATC TGGATAATGC TATTGACTTG 
ATAAAAGAAG CCAATAGCAT CATCATTACT CCAGGCTATG GTCTTTGTGC AGCCAAAGCT 
CAATATCCTA TAGCTGATTT GGTAAAGATG CTCACTGAAC AAGGCAAGAA GGTCAGGTTT 
GGAATCCATC CAGTTGCAGG CCGAATGCCT GGTCAGCTGA ATGTACTTCT AGCTGAAGCT 
GGGGTCCCGT ATGATATTGT ACTAGAAATG GATGAAATCA ATCACGATTT CCCAGATACC 
GATTTAGTCC TTGTAATTGG CGCAAATGAC ACTGTTAATT CAGCAGCTCA AGAAGATCCC 
AATTCAATCA TTGCTGGGAT GCCAGTTTTA GAAGTCTGGA AATCAAAACA GGTAATTGTT 
ATGAAGAGGT CTCTGGGAGT TGGCTATGCT GCAGTGGATA ACCCAATATT CTACAAACCC 
AACACTTCCA TGCTTTTAGG AGATGCCAAG AAGACTTGTG ACGCTCTACA GGCAAAAATT 
AGGGAGTCCT ACCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001364111.1
CDS	174..3434
Translation	MASILKTVVTSYSSPLLINLGSFKVLPGKTEVLRTFHTNQTLWCKAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQTLVKQGFNVVVESGAGEASRFSDDHYKAAGALIQGTREVLASDLVVKVRAPMLNVALGTHEADLLKKSGTLISFIYPAQNPDLLSKLSERKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIIGGGVAGLASAGAAKAMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFTKDMIESMKEGSVVVDLAAEAGGNFETTKPGELYIHKGITHIGYTDLPSRMATQASSLYSNNIIKLLKAISPDKDNFHFEVKDDFDFGTMGHVIRGTVVMKDGKNIFPAPTPKNIPQGAPVKPKTVAELEAEKAATLTPFHKTMTTATAYTAGLTSMLGLGIIAPNAAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGHIYPSNTPQGLATLATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPGGTFVGGYLASLYSGYDIEQMIYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGSLNPNPELLAQMSGAMTLGGTIGLMIAKRIQITDLPQLVAAFHSLVGLAAVLTCVAEYMVEYPHFAVDPAANLTKIVAYLGTYIGGVTFSGSLIAYGKLQGILKSAPLLLPGRHLLNLGLLTASAGGLIPYLLDPSYTMGITCLGSVSALSAIMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGSHTEINLDNAIDLIKEANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTSMLLGDAKKTCDALQAKIRESYQK

Target ORF information:

RefSeq Version	XM_001364074.4
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001364074.4

ORF Insert Sequence:

ATGGCAAGCA TATTGAAAAC AGTGGTGACA AGCTACTCAA GTCCTCTACT CATTAATTTG 
GGATCCTTTA AGGTGCTACC TGGGAAGACA GAAGTTTTAC GAACTTTTCA TACCAATCAG 
ACACTGTGGT GTAAAGCACC TGTAAAACCA GGAATTCCAT ATAAACAATT GACTGTGGGA 
GTACCCAAGG AGATTTTCCA AAATGAGAAG AGAGTGGCTT TGTCACCTGC TGGTGTTCAG 
ACCTTGGTTA AACAGGGTTT TAATGTGGTG GTGGAATCTG GTGCAGGTGA AGCCTCTAGG 
TTTTCTGATG ATCACTACAA AGCTGCAGGA GCTCTAATCC AAGGGACTCG GGAAGTATTA 
GCATCTGATT TAGTTGTCAA AGTTAGAGCT CCCATGCTAA ACGTTGCATT AGGCACCCAT 
GAGGCTGATC TTTTAAAGAA ATCTGGCACC CTGATTAGTT TTATCTACCC AGCTCAAAAT 
CCAGATTTGC TGAGCAAACT TTCTGAAAGA AAAACTACTG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CCATTGCTCA GGGATATGAT GCTCTTAGCT CAATGGCCAA CATTGCAGGT 
TACAAGGCTG TTGTCCTAGC GGCAAATCAT TTTGGTCGTT TTTTCACTGG CCAGATCACA 
GCAGCTGGAA AAGTTCCGCC AGCTAAGATT CTTATCATTG GTGGTGGAGT CGCTGGGCTT 
GCTTCTGCTG GAGCAGCTAA GGCTATGGGG GCAATTGTTC GAGGATTTGA TACCAGAGCT 
GCAGCTTTGG AGCAGTTTAA GTCTCTTGGG GCAGAGCCTT TGGAAGTAGA CTTGAAAGAG 
TCAGGAGAAG GACAAGGAGG GTATGCCAAA GAAATGTCCA AAGAGTTCAT TGAAGCAGAA 
ATGAAACTAT TTGCCCAGCA ATGTAAAGAA GTTGATATTC TCATTAGTAC CGCCCTTATT 
CCTGGTAAAA AAGCTCCGAT TTTGTTTACA AAAGATATGA TTGAATCAAT GAAAGAAGGT 
TCAGTTGTTG TGGATTTAGC AGCTGAAGCA GGAGGAAACT TTGAAACTAC TAAGCCTGGA 
GAACTTTATA TCCATAAGGG AATTACTCAC ATAGGATACA CAGACCTTCC TAGTCGAATG 
GCTACCCAGG CCAGTTCACT GTATTCGAAC AATATTATCA AACTTCTGAA GGCAATTAGT 
CCTGATAAAG ATAATTTCCA TTTTGAGGTG AAGGATGACT TTGATTTTGG CACAATGGGT 
CATGTCATTA GGGGGACCGT GGTAATGAAG GATGGCAAGA ACATTTTCCC AGCACCAACA 
CCAAAAAATA TTCCTCAGGG AGCCCCTGTA AAACCAAAGA CAGTAGCTGA GCTTGAAGCT 
GAGAAAGCAG CTACTCTTAC CCCATTCCAC AAGACCATGA CCACAGCAAC TGCATATACA 
GCAGGTCTAA CTAGTATGCT TGGTTTGGGC ATCATAGCCC CCAATGCAGC CTTTTCTCAG 
ATGGTGACCA CTTTTGGATT AGCTGGCATT GTGGGCTACC ACACAGTGTG GGGTGTCACT 
CCTGCTCTCC ACTCACCACT GATGTCTGTG ACTAATGCTA TCTCAGGTTT GACTGCAGTT 
GGAGGGTTGG CATTAATGGG AGGACACATC TATCCTTCCA ATACTCCTCA GGGTCTCGCT 
ACGCTTGCTA CTTTCATATC CTCTGTCAAC ATTGCAGGTG GTTTCCTGGT GACCCAGAGG 
ATGCTGGACA TGTTCAAACG TCCTACTGAC CCCCCTGAAT ACAATTACTT GTATCTGCTC 
CCAGGTGGTA CCTTTGTTGG GGGTTATTTA GCTTCACTTT ACAGTGGCTA TGATATTGAA 
CAGATGATAT ATCTGGGCTC AGGCCTATGT TGTGTTGGTG CCCTGGCTGG TCTTTCCACA 
CAAGGAACTG CTCGTCTTGG GAATGCCTTG GGCATGATTG GTGTTGCTGG AGGTTTGGCA 
GCTACTCTGG GAAGTCTTAA TCCAAATCCA GAATTGTTAG CTCAGATGTC TGGGGCAATG 
ACTCTGGGTG GAACCATTGG TTTGATGATT GCTAAGCGTA TCCAGATTAC TGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTGGTA GGTTTGGCGG CTGTGTTAAC CTGTGTGGCA 
GAATATATGG TTGAATATCC ACATTTTGCT GTGGATCCAG CAGCAAACCT TACGAAGATT 
GTGGCTTACC TGGGTACTTA TATTGGTGGT GTGACTTTTA GTGGCTCCCT CATTGCCTAT 
GGAAAATTGC AGGGTATATT GAAATCGGCC CCTCTCCTTC TACCTGGAAG GCATTTACTC 
AATTTAGGCT TATTGACTGC TAGTGCAGGT GGATTAATTC CCTACTTGCT TGATCCCAGC 
TACACAATGG GAATTACCTG TCTAGGTTCT GTATCTGCTC TCTCAGCTAT CATGGGTGTG 
ACTTTAACAG CTGCTATTGG TGGTGCTGAC ATGCCCGTAG TCATCACTGT ACTGAATAGC 
TACTCAGGGT GGGCTCTGTG TGCTGAAGGT TTCCTTCTCA ACAACAACTT GCTTACTATT 
GTGGGTGCTC TGATTGGCTC CTCTGGTGCT ATCCTCTCCT ACATCATGTG TGTGGCTATG 
AATCGTTCCC TGGCTAATGT GATCCTTGGA GGCTATGGCA CTACTTCAAC CGCTGGTGGC 
AAACCTATGG AAATTTCTGG CTCACATACT GAAATTAATC TGGATAATGC TATTGACTTG 
ATAAAAGAAG CCAATAGCAT CATCATTACT CCAGGCTATG GTCTTTGTGC AGCCAAAGCT 
CAATATCCTA TAGCTGATTT GGTAAAGATG CTCACTGAAC AAGGCAAGAA GGTCAGGTTT 
GGAATCCATC CAGTTGCAGG CCGAATGCCT GGTCAGCTGA ATGTACTTCT AGCTGAAGCT 
GGGGTCCCGT ATGATATTGT ACTAGAAATG GATGAAATCA ATCACGATTT CCCAGATACC 
GATTTAGTCC TTGTAATTGG CGCAAATGAC ACTGTTAATT CAGCAGCTCA AGAAGATCCC 
AATTCAATCA TTGCTGGGAT GCCAGTTTTA GAAGTCTGGA AATCAAAACA GGTAATTGTT 
ATGAAGAGGT CTCTGGGAGT TGGCTATGCT GCAGTGGATA ACCCAATATT CTACAAACCC 
AACACTTCCA TGCTTTTAGG AGATGCCAAG AAGACTTGTG ACGCTCTACA GGCAAAAATT 
AGGGAGTCCT ACCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_007487387.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007487449.1 Monodelphis domestica nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMc15898
Clone ID Related Accession (Same CDS sequence)	XM_001364074.4 , XM_007487387.2
Accession Version	XM_007487387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NAD(P) transhydrogenase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007487387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA TATTGAAAAC AGTGGTGACA AGCTACTCAA GTCCTCTACT CATTAATTTG 
GGATCCTTTA AGGTGCTACC TGGGAAGACA GAAGTTTTAC GAACTTTTCA TACCAATCAG 
ACACTGTGGT GTAAAGCACC TGTAAAACCA GGAATTCCAT ATAAACAATT GACTGTGGGA 
GTACCCAAGG AGATTTTCCA AAATGAGAAG AGAGTGGCTT TGTCACCTGC TGGTGTTCAG 
ACCTTGGTTA AACAGGGTTT TAATGTGGTG GTGGAATCTG GTGCAGGTGA AGCCTCTAGG 
TTTTCTGATG ATCACTACAA AGCTGCAGGA GCTCTAATCC AAGGGACTCG GGAAGTATTA 
GCATCTGATT TAGTTGTCAA AGTTAGAGCT CCCATGCTAA ACGTTGCATT AGGCACCCAT 
GAGGCTGATC TTTTAAAGAA ATCTGGCACC CTGATTAGTT TTATCTACCC AGCTCAAAAT 
CCAGATTTGC TGAGCAAACT TTCTGAAAGA AAAACTACTG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CCATTGCTCA GGGATATGAT GCTCTTAGCT CAATGGCCAA CATTGCAGGT 
TACAAGGCTG TTGTCCTAGC GGCAAATCAT TTTGGTCGTT TTTTCACTGG CCAGATCACA 
GCAGCTGGAA AAGTTCCGCC AGCTAAGATT CTTATCATTG GTGGTGGAGT CGCTGGGCTT 
GCTTCTGCTG GAGCAGCTAA GGCTATGGGG GCAATTGTTC GAGGATTTGA TACCAGAGCT 
GCAGCTTTGG AGCAGTTTAA GTCTCTTGGG GCAGAGCCTT TGGAAGTAGA CTTGAAAGAG 
TCAGGAGAAG GACAAGGAGG GTATGCCAAA GAAATGTCCA AAGAGTTCAT TGAAGCAGAA 
ATGAAACTAT TTGCCCAGCA ATGTAAAGAA GTTGATATTC TCATTAGTAC CGCCCTTATT 
CCTGGTAAAA AAGCTCCGAT TTTGTTTACA AAAGATATGA TTGAATCAAT GAAAGAAGGT 
TCAGTTGTTG TGGATTTAGC AGCTGAAGCA GGAGGAAACT TTGAAACTAC TAAGCCTGGA 
GAACTTTATA TCCATAAGGG AATTACTCAC ATAGGATACA CAGACCTTCC TAGTCGAATG 
GCTACCCAGG CCAGTTCACT GTATTCGAAC AATATTATCA AACTTCTGAA GGCAATTAGT 
CCTGATAAAG ATAATTTCCA TTTTGAGGTG AAGGATGACT TTGATTTTGG CACAATGGGT 
CATGTCATTA GGGGGACCGT GGTAATGAAG GATGGCAAGA ACATTTTCCC AGCACCAACA 
CCAAAAAATA TTCCTCAGGG AGCCCCTGTA AAACCAAAGA CAGTAGCTGA GCTTGAAGCT 
GAGAAAGCAG CTACTCTTAC CCCATTCCAC AAGACCATGA CCACAGCAAC TGCATATACA 
GCAGGTCTAA CTAGTATGCT TGGTTTGGGC ATCATAGCCC CCAATGCAGC CTTTTCTCAG 
ATGGTGACCA CTTTTGGATT AGCTGGCATT GTGGGCTACC ACACAGTGTG GGGTGTCACT 
CCTGCTCTCC ACTCACCACT GATGTCTGTG ACTAATGCTA TCTCAGGTTT GACTGCAGTT 
GGAGGGTTGG CATTAATGGG AGGACACATC TATCCTTCCA ATACTCCTCA GGGTCTCGCT 
ACGCTTGCTA CTTTCATATC CTCTGTCAAC ATTGCAGGTG GTTTCCTGGT GACCCAGAGG 
ATGCTGGACA TGTTCAAACG TCCTACTGAC CCCCCTGAAT ACAATTACTT GTATCTGCTC 
CCAGGTGGTA CCTTTGTTGG GGGTTATTTA GCTTCACTTT ACAGTGGCTA TGATATTGAA 
CAGATGATAT ATCTGGGCTC AGGCCTATGT TGTGTTGGTG CCCTGGCTGG TCTTTCCACA 
CAAGGAACTG CTCGTCTTGG GAATGCCTTG GGCATGATTG GTGTTGCTGG AGGTTTGGCA 
GCTACTCTGG GAAGTCTTAA TCCAAATCCA GAATTGTTAG CTCAGATGTC TGGGGCAATG 
ACTCTGGGTG GAACCATTGG TTTGATGATT GCTAAGCGTA TCCAGATTAC TGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTGGTA GGTTTGGCGG CTGTGTTAAC CTGTGTGGCA 
GAATATATGG TTGAATATCC ACATTTTGCT GTGGATCCAG CAGCAAACCT TACGAAGATT 
GTGGCTTACC TGGGTACTTA TATTGGTGGT GTGACTTTTA GTGGCTCCCT CATTGCCTAT 
GGAAAATTGC AGGGTATATT GAAATCGGCC CCTCTCCTTC TACCTGGAAG GCATTTACTC 
AATTTAGGCT TATTGACTGC TAGTGCAGGT GGATTAATTC CCTACTTGCT TGATCCCAGC 
TACACAATGG GAATTACCTG TCTAGGTTCT GTATCTGCTC TCTCAGCTAT CATGGGTGTG 
ACTTTAACAG CTGCTATTGG TGGTGCTGAC ATGCCCGTAG TCATCACTGT ACTGAATAGC 
TACTCAGGGT GGGCTCTGTG TGCTGAAGGT TTCCTTCTCA ACAACAACTT GCTTACTATT 
GTGGGTGCTC TGATTGGCTC CTCTGGTGCT ATCCTCTCCT ACATCATGTG TGTGGCTATG 
AATCGTTCCC TGGCTAATGT GATCCTTGGA GGCTATGGCA CTACTTCAAC CGCTGGTGGC 
AAACCTATGG AAATTTCTGG CTCACATACT GAAATTAATC TGGATAATGC TATTGACTTG 
ATAAAAGAAG CCAATAGCAT CATCATTACT CCAGGCTATG GTCTTTGTGC AGCCAAAGCT 
CAATATCCTA TAGCTGATTT GGTAAAGATG CTCACTGAAC AAGGCAAGAA GGTCAGGTTT 
GGAATCCATC CAGTTGCAGG CCGAATGCCT GGTCAGCTGA ATGTACTTCT AGCTGAAGCT 
GGGGTCCCGT ATGATATTGT ACTAGAAATG GATGAAATCA ATCACGATTT CCCAGATACC 
GATTTAGTCC TTGTAATTGG CGCAAATGAC ACTGTTAATT CAGCAGCTCA AGAAGATCCC 
AATTCAATCA TTGCTGGGAT GCCAGTTTTA GAAGTCTGGA AATCAAAACA GGTAATTGTT 
ATGAAGAGGT CTCTGGGAGT TGGCTATGCT GCAGTGGATA ACCCAATATT CTACAAACCC 
AACACTTCCA TGCTTTTAGG AGATGCCAAG AAGACTTGTG ACGCTCTACA GGCAAAAATT 
AGGGAGTCCT ACCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007487449.1
CDS	243..3503
Translation	MASILKTVVTSYSSPLLINLGSFKVLPGKTEVLRTFHTNQTLWCKAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQTLVKQGFNVVVESGAGEASRFSDDHYKAAGALIQGTREVLASDLVVKVRAPMLNVALGTHEADLLKKSGTLISFIYPAQNPDLLSKLSERKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIIGGGVAGLASAGAAKAMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFTKDMIESMKEGSVVVDLAAEAGGNFETTKPGELYIHKGITHIGYTDLPSRMATQASSLYSNNIIKLLKAISPDKDNFHFEVKDDFDFGTMGHVIRGTVVMKDGKNIFPAPTPKNIPQGAPVKPKTVAELEAEKAATLTPFHKTMTTATAYTAGLTSMLGLGIIAPNAAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGHIYPSNTPQGLATLATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPGGTFVGGYLASLYSGYDIEQMIYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGSLNPNPELLAQMSGAMTLGGTIGLMIAKRIQITDLPQLVAAFHSLVGLAAVLTCVAEYMVEYPHFAVDPAANLTKIVAYLGTYIGGVTFSGSLIAYGKLQGILKSAPLLLPGRHLLNLGLLTASAGGLIPYLLDPSYTMGITCLGSVSALSAIMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGSHTEINLDNAIDLIKEANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTSMLLGDAKKTCDALQAKIRESYQK

Target ORF information:

RefSeq Version	XM_007487387.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007487387.2

ORF Insert Sequence:

ATGGCAAGCA TATTGAAAAC AGTGGTGACA AGCTACTCAA GTCCTCTACT CATTAATTTG 
GGATCCTTTA AGGTGCTACC TGGGAAGACA GAAGTTTTAC GAACTTTTCA TACCAATCAG 
ACACTGTGGT GTAAAGCACC TGTAAAACCA GGAATTCCAT ATAAACAATT GACTGTGGGA 
GTACCCAAGG AGATTTTCCA AAATGAGAAG AGAGTGGCTT TGTCACCTGC TGGTGTTCAG 
ACCTTGGTTA AACAGGGTTT TAATGTGGTG GTGGAATCTG GTGCAGGTGA AGCCTCTAGG 
TTTTCTGATG ATCACTACAA AGCTGCAGGA GCTCTAATCC AAGGGACTCG GGAAGTATTA 
GCATCTGATT TAGTTGTCAA AGTTAGAGCT CCCATGCTAA ACGTTGCATT AGGCACCCAT 
GAGGCTGATC TTTTAAAGAA ATCTGGCACC CTGATTAGTT TTATCTACCC AGCTCAAAAT 
CCAGATTTGC TGAGCAAACT TTCTGAAAGA AAAACTACTG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CCATTGCTCA GGGATATGAT GCTCTTAGCT CAATGGCCAA CATTGCAGGT 
TACAAGGCTG TTGTCCTAGC GGCAAATCAT TTTGGTCGTT TTTTCACTGG CCAGATCACA 
GCAGCTGGAA AAGTTCCGCC AGCTAAGATT CTTATCATTG GTGGTGGAGT CGCTGGGCTT 
GCTTCTGCTG GAGCAGCTAA GGCTATGGGG GCAATTGTTC GAGGATTTGA TACCAGAGCT 
GCAGCTTTGG AGCAGTTTAA GTCTCTTGGG GCAGAGCCTT TGGAAGTAGA CTTGAAAGAG 
TCAGGAGAAG GACAAGGAGG GTATGCCAAA GAAATGTCCA AAGAGTTCAT TGAAGCAGAA 
ATGAAACTAT TTGCCCAGCA ATGTAAAGAA GTTGATATTC TCATTAGTAC CGCCCTTATT 
CCTGGTAAAA AAGCTCCGAT TTTGTTTACA AAAGATATGA TTGAATCAAT GAAAGAAGGT 
TCAGTTGTTG TGGATTTAGC AGCTGAAGCA GGAGGAAACT TTGAAACTAC TAAGCCTGGA 
GAACTTTATA TCCATAAGGG AATTACTCAC ATAGGATACA CAGACCTTCC TAGTCGAATG 
GCTACCCAGG CCAGTTCACT GTATTCGAAC AATATTATCA AACTTCTGAA GGCAATTAGT 
CCTGATAAAG ATAATTTCCA TTTTGAGGTG AAGGATGACT TTGATTTTGG CACAATGGGT 
CATGTCATTA GGGGGACCGT GGTAATGAAG GATGGCAAGA ACATTTTCCC AGCACCAACA 
CCAAAAAATA TTCCTCAGGG AGCCCCTGTA AAACCAAAGA CAGTAGCTGA GCTTGAAGCT 
GAGAAAGCAG CTACTCTTAC CCCATTCCAC AAGACCATGA CCACAGCAAC TGCATATACA 
GCAGGTCTAA CTAGTATGCT TGGTTTGGGC ATCATAGCCC CCAATGCAGC CTTTTCTCAG 
ATGGTGACCA CTTTTGGATT AGCTGGCATT GTGGGCTACC ACACAGTGTG GGGTGTCACT 
CCTGCTCTCC ACTCACCACT GATGTCTGTG ACTAATGCTA TCTCAGGTTT GACTGCAGTT 
GGAGGGTTGG CATTAATGGG AGGACACATC TATCCTTCCA ATACTCCTCA GGGTCTCGCT 
ACGCTTGCTA CTTTCATATC CTCTGTCAAC ATTGCAGGTG GTTTCCTGGT GACCCAGAGG 
ATGCTGGACA TGTTCAAACG TCCTACTGAC CCCCCTGAAT ACAATTACTT GTATCTGCTC 
CCAGGTGGTA CCTTTGTTGG GGGTTATTTA GCTTCACTTT ACAGTGGCTA TGATATTGAA 
CAGATGATAT ATCTGGGCTC AGGCCTATGT TGTGTTGGTG CCCTGGCTGG TCTTTCCACA 
CAAGGAACTG CTCGTCTTGG GAATGCCTTG GGCATGATTG GTGTTGCTGG AGGTTTGGCA 
GCTACTCTGG GAAGTCTTAA TCCAAATCCA GAATTGTTAG CTCAGATGTC TGGGGCAATG 
ACTCTGGGTG GAACCATTGG TTTGATGATT GCTAAGCGTA TCCAGATTAC TGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTGGTA GGTTTGGCGG CTGTGTTAAC CTGTGTGGCA 
GAATATATGG TTGAATATCC ACATTTTGCT GTGGATCCAG CAGCAAACCT TACGAAGATT 
GTGGCTTACC TGGGTACTTA TATTGGTGGT GTGACTTTTA GTGGCTCCCT CATTGCCTAT 
GGAAAATTGC AGGGTATATT GAAATCGGCC CCTCTCCTTC TACCTGGAAG GCATTTACTC 
AATTTAGGCT TATTGACTGC TAGTGCAGGT GGATTAATTC CCTACTTGCT TGATCCCAGC 
TACACAATGG GAATTACCTG TCTAGGTTCT GTATCTGCTC TCTCAGCTAT CATGGGTGTG 
ACTTTAACAG CTGCTATTGG TGGTGCTGAC ATGCCCGTAG TCATCACTGT ACTGAATAGC 
TACTCAGGGT GGGCTCTGTG TGCTGAAGGT TTCCTTCTCA ACAACAACTT GCTTACTATT 
GTGGGTGCTC TGATTGGCTC CTCTGGTGCT ATCCTCTCCT ACATCATGTG TGTGGCTATG 
AATCGTTCCC TGGCTAATGT GATCCTTGGA GGCTATGGCA CTACTTCAAC CGCTGGTGGC 
AAACCTATGG AAATTTCTGG CTCACATACT GAAATTAATC TGGATAATGC TATTGACTTG 
ATAAAAGAAG CCAATAGCAT CATCATTACT CCAGGCTATG GTCTTTGTGC AGCCAAAGCT 
CAATATCCTA TAGCTGATTT GGTAAAGATG CTCACTGAAC AAGGCAAGAA GGTCAGGTTT 
GGAATCCATC CAGTTGCAGG CCGAATGCCT GGTCAGCTGA ATGTACTTCT AGCTGAAGCT 
GGGGTCCCGT ATGATATTGT ACTAGAAATG GATGAAATCA ATCACGATTT CCCAGATACC 
GATTTAGTCC TTGTAATTGG CGCAAATGAC ACTGTTAATT CAGCAGCTCA AGAAGATCCC 
AATTCAATCA TTGCTGGGAT GCCAGTTTTA GAAGTCTGGA AATCAAAACA GGTAATTGTT 
ATGAAGAGGT CTCTGGGAGT TGGCTATGCT GCAGTGGATA ACCCAATATT CTACAAACCC 
AACACTTCCA TGCTTTTAGG AGATGCCAAG AAGACTTGTG ACGCTCTACA GGCAAAAATT 
AGGGAGTCCT ACCAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NNT cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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