GPR133 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPR133
Entrez Gene ID	100675988
Full Name	adhesion G protein-coupled receptor D1
Gene Type	protein-coding
Organism	Loxodonta africana(African savanna elephant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023540413.1	XP_023396181.1	LOW QUALITY PROTEIN: adhesion G-protein coupled receptor D1
XM_010600823.1	XP_010599125.1	LOW QUALITY PROTEIN: probable G-protein coupled receptor 133

Pathways from BioSystems
Gene Ontology

The following GPR133 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPR133 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLo108937	XM_023540413.1 Latest version!	Loxodonta africana adhesion G protein-coupled receptor D1 (ADGRD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OLo54840	XM_010600823.1 Latest version!	Loxodonta africana G protein-coupled receptor 133 (GPR133), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPR133 ( XM_023540413.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_023396181.1 Loxodonta africana adhesion G protein-coupled receptor D1 (ADGRD1), mRNA.

Price & Availability↑

CloneID	OLo108937
Clone ID Related Accession (Same CDS sequence)	XM_023540413.1
Accession Version	XM_023540413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	LOW QUALITY PROTEIN: adhesion G-protein coupled receptor D1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573533.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTGGCAGC CACGGCCACT CTACACCTGG TTGCTGCTGT TTTATTCCAC TTCTCAGGTA 
CATGGAGTCG ACGCCAGGTC CCGAGACCAT CCAGGGTTTG AGGTCTTGGC TTCTGCATCA 
CATTACTGGC CGCTGGAACG TGTGGACGGG ATCCACGAGC TTCAGGACAC AACTGGAGCC 
TCCCGAGCCC ACAACCTCAC TGCGCTCCTT CCCCATAACT CCACCTTTGT GTATACCAAT 
GACTCTGCCT ACTCTAACTC CTCAGCGACC GTAGATATTG TAGAAGGGAA GGTCAACAAA 
GGCATTTACC TCAAAGAGGA AAAAGGAGTC ACGCTCCTCT ACTACGGAAG TGTCAGGAAC 
TCCTGCATCA GTGATCCCAG CCAGTGCGGC CCCGAAGGGG TCTCCTTCTC TTTCTTCTGG 
AAGACACAAG GAGAGCTGTC CAGGCCCACT CCCTTTGCCT ATGGGGGGCA GGTGATCTCC 
AACGGGTTCA AGGTCTGCTC CAATGGCGGC AAAGGCTCTG TGGAGCTGTA CACGCGGGAG 
AACTCCATGA CGTGGACAGC CACCTTTAGC CCCCCAGGCC CCTACTGGAC GCATGTGCTA 
TTCACATGGA AATCCAAGGA GGGCCTGAAG GTCTACGTCA ATGGGACCCT GAGCACCTCC 
GACCCCAGCG GGAAGGTGTC CCACGCCTAT GGGGACCCCA ATGCAAACCT GGTGATCGGG 
GCTGAGCAGG ACCAGACCAG GCGCTATGAG AATGGTGCCT TTGATGAGTT CATCATCTGG 
GAGCGGGCAC TGACCCCGGA CGAGGTTGCC ATGTACTTCA CAGCTGCTGT GGGAAAGCAC 
ATGGCATTGT CTTCCACACC ACCAAGCTTC CTTGTGACAC CCACAGTGAG CGCCGTGATG 
CCCACGGATG CCTACCACCC CATCATAACC AACCTGACAG AGGAGAGGAG AAAATTCCAG 
AGCCCTGGGA TTGTGCTGAG TTACCTTCAG AACGTGTCCC TCCACTTACC CAATGAATCC 
CTCTCACAGG AAACGGCCCG GAACCTCACC GAGACCTTCT TAAAAACCGT GGGAGAGGTT 
CTTCTACTGC CCAGCTGGAC TGACGAGTCA GAGGACCAGG CCGCGGTGCT GGGCCTGATT 
GACACCATCG ACACGGTGAT GAGGCACATA TCTGCCAACC TGCACACCCA CGAGCCCCAG 
GTCACCATCG CCGGCTCCTC CTCCATGGCA GAGTACTCAG TGGCCAAGGT CCTCTCCGGA 
ACCATGAATG CCTCCCACTA CCGCTTCCCA GCCCAGGGGC ACAGCTACGT CCAGATCCCC 
CACGAGGCCT TCCACAGCCA AGCCTGGACC ACCATCGGGG GCCTCCTCTA CCACACCGTA 
CACTTCTCCC TGAGCAACAT CCATCCGGAC AACACCAGGA TTGCCGAGGC AGCGAAGCAT 
CAAGGCTGCC TGATGTCAGC CGCCAGCCCC CTGATCTCCC TGGAGGTCTC CCCACCGCCT 
GCTCTTGCCC AGACTTTGTC GGGGTCTCCG CTGGTCACTG TCCACCTCAC ACACATACTG 
ACACAGAAGC AGTACAGCAG GGCCACCAAT GAGAGCAACC AAGTCTTCTG GTACTGCGCC 
TTCTTGGACT TCAGCTCTGG AAAAGGGGTC TGGTCAAACG AGGGCTGTGC CCTCACAGAG 
GGGAACCTCA GCTACTCTGT CTGCAGCTGC ACTCACCTTA CCAACTTCGC CGTCCTGATG 
CAGGTGGTCC CGCTGGAGCT GGCACATGGA CACCAGGTGG CGCTGTCGTC CATCAGTTAC 
ATCGGCTGCT CCCTGTCTGT GATCTGCCTG ACCGCCACAC TGGCCACTTT CGCCGTCCTC 
TCCTCCGTCA GCACCATCAG GAGCCAGCGC TACCATATCC ACGCCAACCT GTCCTTCGCT 
GTGCTGGTGG CCCAGGTGCT GCTGCTTGTC AGCTTCCAGT TCCATCCGGG CACGACCCCA 
TGCAGGGTCC TGGCCGTGCT CCTGCACTAC TTCTTCCTGA GTGCCTTCGC GTGGATGCTG 
GTGGAGGGCT TGCATCTCTA CAGCATGGTC ATCAAGGTCT TTGGCTCAGA GGACAGCAAG 
CACCTCTGCT ACTACGGCGT AGGATGGGGT TTTCCTCTTC TGACCTGTAT CATTTCAGTG 
TCATCTGCCA TGGACAGTTA CGGAGCAAGT GACAACTGCT GGCTGTCCCT GGGGACTGGC 
GCCATCTGGG CCTTCGTGGC TCCAGCGCTG CTGGTGATCG TTGTCAACGT CGGCATCCTT 
GTCGCCGTGA CCCGGGTCAT CTCTCAGATC AGCGCCGACA ACTATAAGAT ACATGGAGAC 
CCCAACGCCT TCAAGCTGAC TGTGAAGGCC GTGGCGGTGC TGCTGCCCAT CCTGGGCACC 
TCGTGGGTCT TCGGCGTGCT TGCAGTCAAC GACCAGGCCG TGGTCTTCCA GTACATGTTC 
GCTGTGCTCA ACTCCTTACA GGGCCTCTTC ATCTTCCTCT TCCACTGCCT CCTGAACTCA 
GAGGTGAGAG CTGCCTTCAA GCACAAAACC AAGATCTGGT CCCTCACCAG CAGCTCTGCC 
CGCAGCACCC ACGCGAAGCC CTTCAGCTCC GACATCATGA ATGGGACCCG GCCTGCCACA 
GCCTCCTCGA AGCTCAGCCC CTGGGACAAG AGCAGCCTCT CCTCCCACCG TGTGGACCTA 
TCTGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023396181.1
CDS	777..3488
Translation	MWQPRPLYTWLLLFYSTSQVHGVDARSRDHPGFEVLASASHYWPLERVDGIHELQDTTGASRAHNLTALLPHNSTFVYTNDSAYSNSSATVDIVEGKVNKGIYLKEEKGVTLLYYGSVRNSCISDPSQCGPEGVSFSFFWKTQGELSRPTPFAYGGQVISNGFKVCSNGGKGSVELYTRENSMTWTATFSPPGPYWTHVLFTWKSKEGLKVYVNGTLSTSDPSGKVSHAYGDPNANLVIGAEQDQTRRYENGAFDEFIIWERALTPDEVAMYFTAAVGKHMALSSTPPSFLVTPTVSAVMPTDAYHPIITNLTEERRKFQSPGIVLSYLQNVSLHLPNESLSQETARNLTETFLKTVGEVLLLPSWTDESEDQAAVLGLIDTIDTVMRHISANLHTHEPQVTIAGSSSMAEYSVAKVLSGTMNASHYRFPAQGHSYVQIPHEAFHSQAWTTIGGLLYHTVHFSLSNIHPDNTRIAEAAKHQGCLMSAASPLISLEVSPPPALAQTLSGSPLVTVHLTHILTQKQYSRATNESNQVFWYCAFLDFSSGKGVWSNEGCALTEGNLSYSVCSCTHLTNFAVLMQVVPLELAHGHQVALSSISYIGCSLSVICLTATLATFAVLSSVSTIRSQRYHIHANLSFAVLVAQVLLLVSFQFHPGTTPCRVLAVLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHLCYYGVGWGFPLLTCIISVSSAMDSYGASDNCWLSLGTGAIWAFVAPALLVIVVNVGILVAVTRVISQISADNYKIHGDPNAFKLTVKAVAVLLPILGTSWVFGVLAVNDQAVVFQYMFAVLNSLQGLFIFLFHCLLNSEVRAAFKHKTKIWSLTSSSARSTHAKPFSSDIMNGTRPATASSKLSPWDKSSLSSHRVDLSAV

Target ORF information:

RefSeq Version	XM_023540413.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana adhesion G protein-coupled receptor D1 (ADGRD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023540413.1

ORF Insert Sequence:

ATGTGGCAGC CACGGCCACT CTACACCTGG TTGCTGCTGT TTTATTCCAC TTCTCAGGTA 
CATGGAGTCG ACGCCAGGTC CCGAGACCAT CCAGGGTTTG AGGTCTTGGC TTCTGCATCA 
CATTACTGGC CGCTGGAACG TGTGGACGGG ATCCACGAGC TTCAGGACAC AACTGGAGCC 
TCCCGAGCCC ACAACCTCAC TGCGCTCCTT CCCCATAACT CCACCTTTGT GTATACCAAT 
GACTCTGCCT ACTCTAACTC CTCAGCGACC GTAGATATTG TAGAAGGGAA GGTCAACAAA 
GGCATTTACC TCAAAGAGGA AAAAGGAGTC ACGCTCCTCT ACTACGGAAG TGTCAGGAAC 
TCCTGCATCA GTGATCCCAG CCAGTGCGGC CCCGAAGGGG TCTCCTTCTC TTTCTTCTGG 
AAGACACAAG GAGAGCTGTC CAGGCCCACT CCCTTTGCCT ATGGGGGGCA GGTGATCTCC 
AACGGGTTCA AGGTCTGCTC CAATGGCGGC AAAGGCTCTG TGGAGCTGTA CACGCGGGAG 
AACTCCATGA CGTGGACAGC CACCTTTAGC CCCCCAGGCC CCTACTGGAC GCATGTGCTA 
TTCACATGGA AATCCAAGGA GGGCCTGAAG GTCTACGTCA ATGGGACCCT GAGCACCTCC 
GACCCCAGCG GGAAGGTGTC CCACGCCTAT GGGGACCCCA ATGCAAACCT GGTGATCGGG 
GCTGAGCAGG ACCAGACCAG GCGCTATGAG AATGGTGCCT TTGATGAGTT CATCATCTGG 
GAGCGGGCAC TGACCCCGGA CGAGGTTGCC ATGTACTTCA CAGCTGCTGT GGGAAAGCAC 
ATGGCATTGT CTTCCACACC ACCAAGCTTC CTTGTGACAC CCACAGTGAG CGCCGTGATG 
CCCACGGATG CCTACCACCC CATCATAACC AACCTGACAG AGGAGAGGAG AAAATTCCAG 
AGCCCTGGGA TTGTGCTGAG TTACCTTCAG AACGTGTCCC TCCACTTACC CAATGAATCC 
CTCTCACAGG AAACGGCCCG GAACCTCACC GAGACCTTCT TAAAAACCGT GGGAGAGGTT 
CTTCTACTGC CCAGCTGGAC TGACGAGTCA GAGGACCAGG CCGCGGTGCT GGGCCTGATT 
GACACCATCG ACACGGTGAT GAGGCACATA TCTGCCAACC TGCACACCCA CGAGCCCCAG 
GTCACCATCG CCGGCTCCTC CTCCATGGCA GAGTACTCAG TGGCCAAGGT CCTCTCCGGA 
ACCATGAATG CCTCCCACTA CCGCTTCCCA GCCCAGGGGC ACAGCTACGT CCAGATCCCC 
CACGAGGCCT TCCACAGCCA AGCCTGGACC ACCATCGGGG GCCTCCTCTA CCACACCGTA 
CACTTCTCCC TGAGCAACAT CCATCCGGAC AACACCAGGA TTGCCGAGGC AGCGAAGCAT 
CAAGGCTGCC TGATGTCAGC CGCCAGCCCC CTGATCTCCC TGGAGGTCTC CCCACCGCCT 
GCTCTTGCCC AGACTTTGTC GGGGTCTCCG CTGGTCACTG TCCACCTCAC ACACATACTG 
ACACAGAAGC AGTACAGCAG GGCCACCAAT GAGAGCAACC AAGTCTTCTG GTACTGCGCC 
TTCTTGGACT TCAGCTCTGG AAAAGGGGTC TGGTCAAACG AGGGCTGTGC CCTCACAGAG 
GGGAACCTCA GCTACTCTGT CTGCAGCTGC ACTCACCTTA CCAACTTCGC CGTCCTGATG 
CAGGTGGTCC CGCTGGAGCT GGCACATGGA CACCAGGTGG CGCTGTCGTC CATCAGTTAC 
ATCGGCTGCT CCCTGTCTGT GATCTGCCTG ACCGCCACAC TGGCCACTTT CGCCGTCCTC 
TCCTCCGTCA GCACCATCAG GAGCCAGCGC TACCATATCC ACGCCAACCT GTCCTTCGCT 
GTGCTGGTGG CCCAGGTGCT GCTGCTTGTC AGCTTCCAGT TCCATCCGGG CACGACCCCA 
TGCAGGGTCC TGGCCGTGCT CCTGCACTAC TTCTTCCTGA GTGCCTTCGC GTGGATGCTG 
GTGGAGGGCT TGCATCTCTA CAGCATGGTC ATCAAGGTCT TTGGCTCAGA GGACAGCAAG 
CACCTCTGCT ACTACGGCGT AGGATGGGGT TTTCCTCTTC TGACCTGTAT CATTTCAGTG 
TCATCTGCCA TGGACAGTTA CGGAGCAAGT GACAACTGCT GGCTGTCCCT GGGGACTGGC 
GCCATCTGGG CCTTCGTGGC TCCAGCGCTG CTGGTGATCG TTGTCAACGT CGGCATCCTT 
GTCGCCGTGA CCCGGGTCAT CTCTCAGATC AGCGCCGACA ACTATAAGAT ACATGGAGAC 
CCCAACGCCT TCAAGCTGAC TGTGAAGGCC GTGGCGGTGC TGCTGCCCAT CCTGGGCACC 
TCGTGGGTCT TCGGCGTGCT TGCAGTCAAC GACCAGGCCG TGGTCTTCCA GTACATGTTC 
GCTGTGCTCA ACTCCTTACA GGGCCTCTTC ATCTTCCTCT TCCACTGCCT CCTGAACTCA 
GAGGTGAGAG CTGCCTTCAA GCACAAAACC AAGATCTGGT CCCTCACCAG CAGCTCTGCC 
CGCAGCACCC ACGCGAAGCC CTTCAGCTCC GACATCATGA ATGGGACCCG GCCTGCCACA 
GCCTCCTCGA AGCTCAGCCC CTGGGACAAG AGCAGCCTCT CCTCCCACCG TGTGGACCTA 
TCTGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR133 ( XM_010600823.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_010599125.1 Loxodonta africana G protein-coupled receptor 133 (GPR133), mRNA.

Price & Availability↑

CloneID	OLo54840
Clone ID Related Accession (Same CDS sequence)	XM_010600823.1
Accession Version	XM_010600823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1418140800000
Organism	Loxodonta africana(African savanna elephant)
Product	LOW QUALITY PROTEIN: probable G-protein coupled receptor 133
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573533.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Loxodonta africana Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTGGCAGC CACGGCCACT CTACACCTGG TTGCTGCTGT TTTATTCCAC TTCTCAGGTA 
CATGGAGTCG ACGCCAGGTC CCGAGACCAT CCAGGGTTTG AGGTCTTGGC TTCTGCATCA 
CATTACTGGC CGCTGGAACG TGTGGACGGG ATCCACGAGC TTCAGGACAC AACTGGAGCC 
TCCCGAGCCC ACAACCTCAC TGCGCTCCTT CCCCATAACT CCACCTTTGT GTATACCAAT 
GACTCTGCCT ACTCTAACTC CTCAGCGACC GTAGATATTG TAGAAGGGAA GGTCAACAAA 
GGCATTTACC TCAAAGAGGA AAAAGGAGTC ACGCTCCTCT ACTACGGAAG TGTCAGGAAC 
TCCTGCATCA GTGATCCCAG CCAGTGCGGC CCCGAAGGGG TCTCCTTCTC TTTCTTCTGG 
AAGACACAAG GAGAGCTGTC CAGGCCCACT CCCTTTGCCT ATGGGGGGCA GGTGATCTCC 
AACGGGTTCA AGGTCTGCTC CAATGGCGGC AAAGGCTCTG TGGAGCTGTA CACGCGGGAG 
AACTCCATGA CGTGGACAGC CACCTTTAGC CCCCCAGGCC CCTACTGGAC GCATGTGCTA 
TTCACATGGA AATCCAAGGA GGGCCTGAAG GTCTACGTCA ATGGGACCCT GAGCACCTCC 
GACCCCAGCG GGAAGGTGTC CCACGCCTAT GGGGACCCCA ATGCAAACCT GGTGATCGGG 
GCTGAGCAGG ACCAGACCAG GCGCTATGAG AATGGTGCCT TTGATGAGTT CATCATCTGG 
GAGCGGGCAC TGACCCCGGA CGAGGTTGCC ATGTACTTCA CAGCTGCTGT GGGAAAGCAC 
ATGGCATTGT CTTCCACACC ACCAAGCTTC CTTGTGACAC CCACAGTGAG CGCCGTGATG 
CCCACGGATG CCTACCACCC CATCATAACC AACCTGACAG AGGAGAGGAG AAAATTCCAG 
AGCCCTGGGA TTGTGCTGAG TTACCTTCAG AACGTGTCCC TCCACTTACC CAATGAATCC 
CTCTCACAGG AAACGGCCCG GAACCTCACC GAGACCTTCT TAAAAACCGT GGGAGAGGTT 
CTTCTACTGC CCAGCTGGAC TGACGAGTCA GAGGACCAGG CCGCGGTGCT GGGCCTGATT 
GACACCATCG ACACGGTGAT GAGGCACATA TCTGCCAACC TGCACACCCA CGAGCCCCAG 
GTCACCATCG CCGGCTCCTC CTCCATGGCA GAGTACTCAG TGGCCAAGGT CCTCTCCGGA 
ACCATGAATG CCTCCCACTA CCGCTTCCCA GCCCAGGGGC ACAGCTACGT CCAGATCCCC 
CACGAGGCCT TCCACAGCCA AGCCTGGACC ACCATCGGGG GCCTCCTCTA CCACACCGTA 
CACTTCTCCC TGAGCAACAT CCATCCGGAC AACACCAGGA TTGCCGAGGC AGCGAAGCAT 
CAAGGCTGCC TGATGTCAGC CGCCAGCCCC CTGATCTCCC TGGAGGTCTC CCCACCGCCT 
GCTCTTGCCC AGACTTTGTC GGGGTCTCCG CTGGTCACTG TCCACCTCAC ACACATACTG 
ACACAGAAGC AGTACAGCAG GGCCACCAAT GAGAGCAACC AAGTCTTCTG GTACTGCGCC 
TTCTTGGACT TCAGCTCTGG AAAAGGGGTC TGGTCAAACG AGGGCTGTGC CCTCACAGAG 
GGGAACCTCA GCTACTCTGT CTGCAGCTGC ACTCACCTTA CCAACTTCGC CGTCCTGATG 
CAGGTGGTCC CGCTGGAGCT GGCACATGGA CACCAGGTGG CGCTGTCGTC CATCAGTTAC 
ATCGGCTGCT CCCTGTCTGT GATCTGCCTG ACCGCCACAC TGGCCACTTT CGCCGTCCTC 
TCCTCCGTCA GCACCATCAG GAGCCAGCGC TACCATATCC ACGCCAACCT GTCCTTCGCT 
GTGCTGGTGG CCCAGGTGCT GCTGCTTGTC AGCTTCCAGT TCCATCCGGG CACGACCCCA 
TGCAGGGTCC TGGCCGTGCT CCTGCACTAC TTCTTCCTGA GTGCCTTCGC GTGGATGCTG 
GTGGAGGGCT TGCATCTCTA CAGCATGGTC ATCAAGGTCT TTGGCTCAGA GGACAGCAAG 
CACCTCTGCT ACTACGGCGT AGGATGGGGT TTTCCTCTTC TGACCTGTAT CATTTCAGTG 
TCATCTGCCA TGGACAGTTA CGGAGCAAGT CACAACTGCT GGCTGTCCCT GGGGACTGGC 
GCCATCTGGG CCTTCGTGGC TCCAGCGCTG CTGGTGATCG TTGTCAACGT CGGCATCCTT 
GTCGCCGTGA CCCGGGTCAT CTCTCAGATC AGCGCCGACA ACTATAAGAT ACATGGAGAC 
CCCAACGCCT TCAAGCTGAC TGTGAAGGCC GTGGCGGTGC TGCTGCCCAT CCTGGGCACC 
TCGTGGGTCT TCGGCGTGCT TGCAGTCAAC GACCAGGCCG TGGTCTTCCA GTACATGTTC 
GCTGTGCTCA ACTCCTTACA GGGCCTCTTC ATCTTCCTCT TCCACTGCCT CCTGAACTCA 
GAGGTGAGAG CTGCCTTCAA GCACAAAACC AAGATCTGGT CCCTCACCAG CAGCTCTGCC 
CGCAGCACCC ACGCGAAGCC CTTCAGCTCC GACATCATGA ATGGGACCCG GCCTGCCACA 
GCCTCCTCGA AGCTCAGCCC CTGGGACAAG AGCAGCCTCT CCTCCCACCG TGTGGACCTA 
TCTGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010599125.1
CDS	1..2712
Translation	MWQPRPLYTWLLLFYSTSQVHGVDARSRDHPGFEVLASASHYWPLERVDGIHELQDTTGASRAHNLTALLPHNSTFVYTNDSAYSNSSATVDIVEGKVNKGIYLKEEKGVTLLYYGSVRNSCISDPSQCGPEGVSFSFFWKTQGELSRPTPFAYGGQVISNGFKVCSNGGKGSVELYTRENSMTWTATFSPPGPYWTHVLFTWKSKEGLKVYVNGTLSTSDPSGKVSHAYGDPNANLVIGAEQDQTRRYENGAFDEFIIWERALTPDEVAMYFTAAVGKHMALSSTPPSFLVTPTVSAVMPTDAYHPIITNLTEERRKFQSPGIVLSYLQNVSLHLPNESLSQETARNLTETFLKTVGEVLLLPSWTDESEDQAAVLGLIDTIDTVMRHISANLHTHEPQVTIAGSSSMAEYSVAKVLSGTMNASHYRFPAQGHSYVQIPHEAFHSQAWTTIGGLLYHTVHFSLSNIHPDNTRIAEAAKHQGCLMSAASPLISLEVSPPPALAQTLSGSPLVTVHLTHILTQKQYSRATNESNQVFWYCAFLDFSSGKGVWSNEGCALTEGNLSYSVCSCTHLTNFAVLMQVVPLELAHGHQVALSSISYIGCSLSVICLTATLATFAVLSSVSTIRSQRYHIHANLSFAVLVAQVLLLVSFQFHPGTTPCRVLAVLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHLCYYGVGWGFPLLTCIISVSSAMDSYGASHNCWLSLGTGAIWAFVAPALLVIVVNVGILVAVTRVISQISADNYKIHGDPNAFKLTVKAVAVLLPILGTSWVFGVLAVNDQAVVFQYMFAVLNSLQGLFIFLFHCLLNSEVRAAFKHKTKIWSLTSSSARSTHAKPFSSDIMNGTRPATASSKLSPWDKSSLSSHRVDLSAV

Target ORF information:

RefSeq Version	XM_010600823.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana G protein-coupled receptor 133 (GPR133), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010600823.1

ORF Insert Sequence:

ATGTGGCAGC CACGGCCACT CTACACCTGG TTGCTGCTGT TTTATTCCAC TTCTCAGGTA 
CATGGAGTCG ACGCCAGGTC CCGAGACCAT CCAGGGTTTG AGGTCTTGGC TTCTGCATCA 
CATTACTGGC CGCTGGAACG TGTGGACGGG ATCCACGAGC TTCAGGACAC AACTGGAGCC 
TCCCGAGCCC ACAACCTCAC TGCGCTCCTT CCCCATAACT CCACCTTTGT GTATACCAAT 
GACTCTGCCT ACTCTAACTC CTCAGCGACC GTAGATATTG TAGAAGGGAA GGTCAACAAA 
GGCATTTACC TCAAAGAGGA AAAAGGAGTC ACGCTCCTCT ACTACGGAAG TGTCAGGAAC 
TCCTGCATCA GTGATCCCAG CCAGTGCGGC CCCGAAGGGG TCTCCTTCTC TTTCTTCTGG 
AAGACACAAG GAGAGCTGTC CAGGCCCACT CCCTTTGCCT ATGGGGGGCA GGTGATCTCC 
AACGGGTTCA AGGTCTGCTC CAATGGCGGC AAAGGCTCTG TGGAGCTGTA CACGCGGGAG 
AACTCCATGA CGTGGACAGC CACCTTTAGC CCCCCAGGCC CCTACTGGAC GCATGTGCTA 
TTCACATGGA AATCCAAGGA GGGCCTGAAG GTCTACGTCA ATGGGACCCT GAGCACCTCC 
GACCCCAGCG GGAAGGTGTC CCACGCCTAT GGGGACCCCA ATGCAAACCT GGTGATCGGG 
GCTGAGCAGG ACCAGACCAG GCGCTATGAG AATGGTGCCT TTGATGAGTT CATCATCTGG 
GAGCGGGCAC TGACCCCGGA CGAGGTTGCC ATGTACTTCA CAGCTGCTGT GGGAAAGCAC 
ATGGCATTGT CTTCCACACC ACCAAGCTTC CTTGTGACAC CCACAGTGAG CGCCGTGATG 
CCCACGGATG CCTACCACCC CATCATAACC AACCTGACAG AGGAGAGGAG AAAATTCCAG 
AGCCCTGGGA TTGTGCTGAG TTACCTTCAG AACGTGTCCC TCCACTTACC CAATGAATCC 
CTCTCACAGG AAACGGCCCG GAACCTCACC GAGACCTTCT TAAAAACCGT GGGAGAGGTT 
CTTCTACTGC CCAGCTGGAC TGACGAGTCA GAGGACCAGG CCGCGGTGCT GGGCCTGATT 
GACACCATCG ACACGGTGAT GAGGCACATA TCTGCCAACC TGCACACCCA CGAGCCCCAG 
GTCACCATCG CCGGCTCCTC CTCCATGGCA GAGTACTCAG TGGCCAAGGT CCTCTCCGGA 
ACCATGAATG CCTCCCACTA CCGCTTCCCA GCCCAGGGGC ACAGCTACGT CCAGATCCCC 
CACGAGGCCT TCCACAGCCA AGCCTGGACC ACCATCGGGG GCCTCCTCTA CCACACCGTA 
CACTTCTCCC TGAGCAACAT CCATCCGGAC AACACCAGGA TTGCCGAGGC AGCGAAGCAT 
CAAGGCTGCC TGATGTCAGC CGCCAGCCCC CTGATCTCCC TGGAGGTCTC CCCACCGCCT 
GCTCTTGCCC AGACTTTGTC GGGGTCTCCG CTGGTCACTG TCCACCTCAC ACACATACTG 
ACACAGAAGC AGTACAGCAG GGCCACCAAT GAGAGCAACC AAGTCTTCTG GTACTGCGCC 
TTCTTGGACT TCAGCTCTGG AAAAGGGGTC TGGTCAAACG AGGGCTGTGC CCTCACAGAG 
GGGAACCTCA GCTACTCTGT CTGCAGCTGC ACTCACCTTA CCAACTTCGC CGTCCTGATG 
CAGGTGGTCC CGCTGGAGCT GGCACATGGA CACCAGGTGG CGCTGTCGTC CATCAGTTAC 
ATCGGCTGCT CCCTGTCTGT GATCTGCCTG ACCGCCACAC TGGCCACTTT CGCCGTCCTC 
TCCTCCGTCA GCACCATCAG GAGCCAGCGC TACCATATCC ACGCCAACCT GTCCTTCGCT 
GTGCTGGTGG CCCAGGTGCT GCTGCTTGTC AGCTTCCAGT TCCATCCGGG CACGACCCCA 
TGCAGGGTCC TGGCCGTGCT CCTGCACTAC TTCTTCCTGA GTGCCTTCGC GTGGATGCTG 
GTGGAGGGCT TGCATCTCTA CAGCATGGTC ATCAAGGTCT TTGGCTCAGA GGACAGCAAG 
CACCTCTGCT ACTACGGCGT AGGATGGGGT TTTCCTCTTC TGACCTGTAT CATTTCAGTG 
TCATCTGCCA TGGACAGTTA CGGAGCAAGT CACAACTGCT GGCTGTCCCT GGGGACTGGC 
GCCATCTGGG CCTTCGTGGC TCCAGCGCTG CTGGTGATCG TTGTCAACGT CGGCATCCTT 
GTCGCCGTGA CCCGGGTCAT CTCTCAGATC AGCGCCGACA ACTATAAGAT ACATGGAGAC 
CCCAACGCCT TCAAGCTGAC TGTGAAGGCC GTGGCGGTGC TGCTGCCCAT CCTGGGCACC 
TCGTGGGTCT TCGGCGTGCT TGCAGTCAAC GACCAGGCCG TGGTCTTCCA GTACATGTTC 
GCTGTGCTCA ACTCCTTACA GGGCCTCTTC ATCTTCCTCT TCCACTGCCT CCTGAACTCA 
GAGGTGAGAG CTGCCTTCAA GCACAAAACC AAGATCTGGT CCCTCACCAG CAGCTCTGCC 
CGCAGCACCC ACGCGAAGCC CTTCAGCTCC GACATCATGA ATGGGACCCG GCCTGCCACA 
GCCTCCTCGA AGCTCAGCCC CTGGGACAAG AGCAGCCTCT CCTCCCACCG TGTGGACCTA 
TCTGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GPR133 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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