ORF » Species Summary » Leishmania major strain Friedlin » LMJF_33_2660 cDNA ORF clone

LMJF_33_2660 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_33_2660 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_33_2660 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb05089 XM_001685976.1
Latest version!		 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_33_2660 ( XM_001685976.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001686028.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.
    Price & Availability↑
    CloneID OLb05089
    Clone ID Related Accession (Same CDS sequence) XM_001685976.1
    Accession Version XM_001685976.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product conserved hypothetical protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007285). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACCTCCC GCATCCCACT CCCCCTGCGG CTGGTGCAGT CTGACTTGTG GAGTGCCACG 
    GCGAGCGGCA GGAGCAGCGC CGGTTCCCGT GTCCTGACTG CGTACAAACC GAGCGGTCTC 
    CCGTGCTATG CCACGTCGCA GGTGAGCGCG GTCGCCTCCA GCGGTGGCGA TGGGCGCCGC 
    CTCTGCGCCA TTCACACACG ACACACATCC TCTGCATCTT CCTCGGCCAT TGCTGTCGAG 
    GCGGGACCAC AGCCGCACCC TGAAGACCCC AGCGACTCCC TCCTTACCCG TGTCGTCGAC 
    GCCGCGCTTC CAGGGTGCGT GCCGTACGTG GCACTGCCGG TTGTGTCGTC TCTCCTGCGC 
    CGCGGGGGTC ACGACACCGT CGCAGCCGCG CAGCTGAACC AGTGGACTAG TCGCCACAAA 
    GGCCTTGTGG TGGTGACAGG GACGCCGAGC GACGCCGTGT TCCTTCGCAA CGCCGCACAG 
    CTCGACCTTG TGCAACAGAC GTACCGGGTG GTGTGTCGCC TACCACCAGG CGTTGGCGCC 
    GCCGCGCGGC GGTATTTGCG CGCGCATCAC AGCTGCCAGC AGCCGCGACA GGGTAGCGAT 
    GGGGCCTCTA CGGCGAAGGG GATGCTGAAC CCATACGTGC AGGCGCACAT GGAGCGACGG 
    CGTCAGCGAC AGCAGCTCGC CGACCTTACA ATTGGCATTC CGCTGAGCGC CGAGTTTCCT 
    CCTGCCACAG CGAAGGCTAC AGCGACTGCG GTGTCGACGC TTGCAAGTGA CGCCCGGCTT 
    CCAGCCAGCG TACGCCCGCA CCCGCTCGTC GACGGAGGCT TTTTTGATGT GGCGCGTGGG 
    CAGCTTCGCG TGCAAGGCAC CGTGAGGTGC TATATACGGG CTCGGGCGCC CCTGACAGCA 
    TCCTCAGCAG CGCGCGTGCA CCAGCGGTCG CGGCTGCACA GTCTTTTCTC CGCCCCGGAG 
    GCTGTCACAC CTGGCCACGG CAGCGGCGCA TTCCGCCCAG ACGAGGACCT GAACGAGTCT 
    CAGCACCCCT GGATGCGGCC GGGGTGGCGC CTCGGCGTGT CCCTTGATAT ACCGCACATG 
    CAGTGCGACG TAGCAGCAGC GGGCGGCGAC AATGTGGATC GCCCCCGCCG CACGAGCGTT 
    CCGGCGCCGC GGGGCAAGTC GCTGTCGATG GAATTCTGTT TACTGTCCCT TTCCCCGCGC 
    GACGACAGCG ATGTGGCGTT GTACGAGGTG CACACCCACG GCGACACCAC CGCCGACGAG 
    GTGGCCGCGG TCTTCCACGC AGAGGGCCTG CGCGTGGTGA ATGACTACGT GCAAGATGTG 
    CCGTTGGCGG TGGCAGTGGA GGAGGTGGCA GCGTGCATGC GAGCGGCTCC ACCTGGCCTG 
    CTGCACGAGC TCCCACTTGG GCTGCAGCAC CGCCTTCGCT CGGCGACGGC GGAGGAGCTG 
    GTGGCGCTGC CACTGATGCG AATGCCACTC GAGGATGCCG ATCTCCTTGC TGCGCAGCAC 
    ACGCTCGCAC ATCTGTCCTC ACTCTCGCCA TCGCTGCCGT TTGCTGACGA TGTGGAGCGG 
    CTGCGGCAGG TGGTGATGAC GGCGGCGTCG TCCGCAAGCG GCAGTCCACG CCACCCCCTC 
    CAGCGCCTTC TTCTCAACGC TCTATCGTCG TTGAAGCTGA CAAACGAGGC GGAGCGCCGC 
    GTGTACGAGC AGGTGCTTGC GTTGACCCTC GGCAGCGGCG TCGAGTGCGC TGGCGTTGTC 
    TTCCCAGATC CATCGGACGC AGGCAACGTG CACGTCTTGC AGCAGCTTTG GCTCACCTAT 
    GAGCAGCAGC GACAGCGACA GCCGTCCTTG GCCGATGCGG CGCGCGCGGC GCGACAGCAC 
    CCGCTCGCCT CTTCGCTACG GTACACCACC CGCAGTGTCC TCGACGATCC TGCTGGGCTA 
    GCCCGTGTGC CGCTTCTCCA GGATGTGGCA ATGGGCTTCG CGTCAGATGG CGCTGCGGAG 
    TCGTTGAGTG CGCGTGATTG CAACGGCAGC GTTCCTGGCG GTCGCGGCGA GTGCAGCTTC 
    ACCCAGGCGG CGGAGCTGCT CAGCGCGCTC TCTCACTCGC AGAATGTAGA GGCAGGGCAC 
    AGGTTATCTG GCGACGAGGC GTGCGAGACG GCGGCGTCGG CTCTGACGCG TCTCAATGAC 
    GCGGCACCGC GATGGATGCA GACTGAGGTT CCGTTGCCGT ATGTGGCGGC CTTTCAAGAG 
    TGGTGCGCCG CGTGCCTGGC TCCGTCGTGC TGCACAGATA CAGCTGCCCC TGACGCGGGT 
    GTGGCCGCTC TCTTGCCGTG GCCTGAGAGC CTGCTACGTC GCTGCAACGG CCCAGGCACA 
    TCAAAAGGGT GTGGATCCGA CCGTGTCGCG GAAAGCCATC GCTCAAGCGA TGACGGCGGC 
    CACCCAGCAG TGCCACCGCG GGAGACGCTC CTGGAGCATG TTCGCCTTCC CGTGCGTGTG 
    TTCCTGCGTG TGGAGGAGCT GGGAGAGCTG CGGTGCGCGT ACTGTGGAGG GGTGGGGCAC 
    ACGTGGCAGC ACTGCGTCGC GCGCGTCGCA GAGGCTGTGG CCGTGATGGA AGATGGCGGT 
    CTGGTGGCCA AGTCGTTGCC ACTCCCCGAG AGCACCGCAA GCACTGCTGT TGGACTACCT 
    GTTTCCCAGC CCGCGCCGGC ACCTCTGGCG ACCTCGTTGA TGGACAGCGA AGGTATCACC 
    TCGACCGACG ACGCTGCGGC TGCACTCGCG GAGCAGGCGA ACGCTCTCAC GCGGCATCGT 
    CAGTACCAGC AGCAACATGT CGGGAGAATC GGCTACGATG GTGATGCCTC TGCGTCGCCG 
    TCAGCGTCCT CCGCACCATT CGCAGTTGTC GCCCCGCTCA TTGCTGTGCC CAATGTCGCT 
    GCAACCCACC GCTTCAAGGC TGGCCACGCG CTGCGCAGCG AGTCTCGCAA GCCTGCCGTG 
    CACCGTCGTG TCATGCGGTG TGTGTACTGC GGTGGCCGTC ATCACGTGAC GGCGTGTCCG 
    GCGCTGCGGG CGCAGGACAG CGAGTCCGAG GCGCGCCGCG CCGACGCTGC AGCGGTCGCT 
    TCGCCAGACT CGACCTCTTC GACGTCGTCT CCGCATCACA GCGGGCCACC ACTCTTCTGC 
    ATCAAGTGTG GACAAACCGG CCACCTCTAC ACGCGCTGCC GTGAGGTGCC GGTAGGCCTT 
    CACCACGCGA CGCACTGCCC CATCTGCCTC CAGCTGCGCA CCAGCGGGTC GCACGATCCG 
    GCGCATTGTC CTCGGGGGGT GCCCGTGCCA GACGGCTACC GACTCAACGG GATACCAGTA 
    GCGGAGGAGG GCAGCACTCA GCGTAAAGCA TCCAACTGGC AGTCATCGAG CCAGCCACAG 
    GCGCATTCAG AGGACGGCTA TCGTCCAGCG ACGGCTGACC GACAGTCGTG GCGGGGCAGT 
    ACACATGTTG GCGGGGTGCC TCGCCGTGGC CGACGCCGCG CCTCTGTGTT AATAGCGGAC 
    AGCTTTGTAG ACAGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001686028.1
    CDS1..3501
    Translation

    Target ORF information:

    RefSeq Version XM_001685976.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001685976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACCTCCC GCATCCCACT CCCCCTGCGG CTGGTGCAGT CTGACTTGTG GAGTGCCACG 
    GCGAGCGGCA GGAGCAGCGC CGGTTCCCGT GTCCTGACTG CGTACAAACC GAGCGGTCTC 
    CCGTGCTATG CCACGTCGCA GGTGAGCGCG GTCGCCTCCA GCGGTGGCGA TGGGCGCCGC 
    CTCTGCGCCA TTCACACACG ACACACATCC TCTGCATCTT CCTCGGCCAT TGCTGTCGAG 
    GCGGGACCAC AGCCGCACCC TGAAGACCCC AGCGACTCCC TCCTTACCCG TGTCGTCGAC 
    GCCGCGCTTC CAGGGTGCGT GCCGTACGTG GCACTGCCGG TTGTGTCGTC TCTCCTGCGC 
    CGCGGGGGTC ACGACACCGT CGCAGCCGCG CAGCTGAACC AGTGGACTAG TCGCCACAAA 
    GGCCTTGTGG TGGTGACAGG GACGCCGAGC GACGCCGTGT TCCTTCGCAA CGCCGCACAG 
    CTCGACCTTG TGCAACAGAC GTACCGGGTG GTGTGTCGCC TACCACCAGG CGTTGGCGCC 
    GCCGCGCGGC GGTATTTGCG CGCGCATCAC AGCTGCCAGC AGCCGCGACA GGGTAGCGAT 
    GGGGCCTCTA CGGCGAAGGG GATGCTGAAC CCATACGTGC AGGCGCACAT GGAGCGACGG 
    CGTCAGCGAC AGCAGCTCGC CGACCTTACA ATTGGCATTC CGCTGAGCGC CGAGTTTCCT 
    CCTGCCACAG CGAAGGCTAC AGCGACTGCG GTGTCGACGC TTGCAAGTGA CGCCCGGCTT 
    CCAGCCAGCG TACGCCCGCA CCCGCTCGTC GACGGAGGCT TTTTTGATGT GGCGCGTGGG 
    CAGCTTCGCG TGCAAGGCAC CGTGAGGTGC TATATACGGG CTCGGGCGCC CCTGACAGCA 
    TCCTCAGCAG CGCGCGTGCA CCAGCGGTCG CGGCTGCACA GTCTTTTCTC CGCCCCGGAG 
    GCTGTCACAC CTGGCCACGG CAGCGGCGCA TTCCGCCCAG ACGAGGACCT GAACGAGTCT 
    CAGCACCCCT GGATGCGGCC GGGGTGGCGC CTCGGCGTGT CCCTTGATAT ACCGCACATG 
    CAGTGCGACG TAGCAGCAGC GGGCGGCGAC AATGTGGATC GCCCCCGCCG CACGAGCGTT 
    CCGGCGCCGC GGGGCAAGTC GCTGTCGATG GAATTCTGTT TACTGTCCCT TTCCCCGCGC 
    GACGACAGCG ATGTGGCGTT GTACGAGGTG CACACCCACG GCGACACCAC CGCCGACGAG 
    GTGGCCGCGG TCTTCCACGC AGAGGGCCTG CGCGTGGTGA ATGACTACGT GCAAGATGTG 
    CCGTTGGCGG TGGCAGTGGA GGAGGTGGCA GCGTGCATGC GAGCGGCTCC ACCTGGCCTG 
    CTGCACGAGC TCCCACTTGG GCTGCAGCAC CGCCTTCGCT CGGCGACGGC GGAGGAGCTG 
    GTGGCGCTGC CACTGATGCG AATGCCACTC GAGGATGCCG ATCTCCTTGC TGCGCAGCAC 
    ACGCTCGCAC ATCTGTCCTC ACTCTCGCCA TCGCTGCCGT TTGCTGACGA TGTGGAGCGG 
    CTGCGGCAGG TGGTGATGAC GGCGGCGTCG TCCGCAAGCG GCAGTCCACG CCACCCCCTC 
    CAGCGCCTTC TTCTCAACGC TCTATCGTCG TTGAAGCTGA CAAACGAGGC GGAGCGCCGC 
    GTGTACGAGC AGGTGCTTGC GTTGACCCTC GGCAGCGGCG TCGAGTGCGC TGGCGTTGTC 
    TTCCCAGATC CATCGGACGC AGGCAACGTG CACGTCTTGC AGCAGCTTTG GCTCACCTAT 
    GAGCAGCAGC GACAGCGACA GCCGTCCTTG GCCGATGCGG CGCGCGCGGC GCGACAGCAC 
    CCGCTCGCCT CTTCGCTACG GTACACCACC CGCAGTGTCC TCGACGATCC TGCTGGGCTA 
    GCCCGTGTGC CGCTTCTCCA GGATGTGGCA ATGGGCTTCG CGTCAGATGG CGCTGCGGAG 
    TCGTTGAGTG CGCGTGATTG CAACGGCAGC GTTCCTGGCG GTCGCGGCGA GTGCAGCTTC 
    ACCCAGGCGG CGGAGCTGCT CAGCGCGCTC TCTCACTCGC AGAATGTAGA GGCAGGGCAC 
    AGGTTATCTG GCGACGAGGC GTGCGAGACG GCGGCGTCGG CTCTGACGCG TCTCAATGAC 
    GCGGCACCGC GATGGATGCA GACTGAGGTT CCGTTGCCGT ATGTGGCGGC CTTTCAAGAG 
    TGGTGCGCCG CGTGCCTGGC TCCGTCGTGC TGCACAGATA CAGCTGCCCC TGACGCGGGT 
    GTGGCCGCTC TCTTGCCGTG GCCTGAGAGC CTGCTACGTC GCTGCAACGG CCCAGGCACA 
    TCAAAAGGGT GTGGATCCGA CCGTGTCGCG GAAAGCCATC GCTCAAGCGA TGACGGCGGC 
    CACCCAGCAG TGCCACCGCG GGAGACGCTC CTGGAGCATG TTCGCCTTCC CGTGCGTGTG 
    TTCCTGCGTG TGGAGGAGCT GGGAGAGCTG CGGTGCGCGT ACTGTGGAGG GGTGGGGCAC 
    ACGTGGCAGC ACTGCGTCGC GCGCGTCGCA GAGGCTGTGG CCGTGATGGA AGATGGCGGT 
    CTGGTGGCCA AGTCGTTGCC ACTCCCCGAG AGCACCGCAA GCACTGCTGT TGGACTACCT 
    GTTTCCCAGC CCGCGCCGGC ACCTCTGGCG ACCTCGTTGA TGGACAGCGA AGGTATCACC 
    TCGACCGACG ACGCTGCGGC TGCACTCGCG GAGCAGGCGA ACGCTCTCAC GCGGCATCGT 
    CAGTACCAGC AGCAACATGT CGGGAGAATC GGCTACGATG GTGATGCCTC TGCGTCGCCG 
    TCAGCGTCCT CCGCACCATT CGCAGTTGTC GCCCCGCTCA TTGCTGTGCC CAATGTCGCT 
    GCAACCCACC GCTTCAAGGC TGGCCACGCG CTGCGCAGCG AGTCTCGCAA GCCTGCCGTG 
    CACCGTCGTG TCATGCGGTG TGTGTACTGC GGTGGCCGTC ATCACGTGAC GGCGTGTCCG 
    GCGCTGCGGG CGCAGGACAG CGAGTCCGAG GCGCGCCGCG CCGACGCTGC AGCGGTCGCT 
    TCGCCAGACT CGACCTCTTC GACGTCGTCT CCGCATCACA GCGGGCCACC ACTCTTCTGC 
    ATCAAGTGTG GACAAACCGG CCACCTCTAC ACGCGCTGCC GTGAGGTGCC GGTAGGCCTT 
    CACCACGCGA CGCACTGCCC CATCTGCCTC CAGCTGCGCA CCAGCGGGTC GCACGATCCG 
    GCGCATTGTC CTCGGGGGGT GCCCGTGCCA GACGGCTACC GACTCAACGG GATACCAGTA 
    GCGGAGGAGG GCAGCACTCA GCGTAAAGCA TCCAACTGGC AGTCATCGAG CCAGCCACAG 
    GCGCATTCAG AGGACGGCTA TCGTCCAGCG ACGGCTGACC GACAGTCGTG GCGGGGCAGT 
    ACACATGTTG GCGGGGTGCC TCGCCGTGGC CGACGCCGCG CCTCTGTGTT AATAGCGGAC 
    AGCTTTGTAG ACAGCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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