GCVL-1 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	GCVL-1
Entrez Gene ID	12981045
Full Name	putative dihydrolipoamide dehydrogenase
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_003722217.1	XP_003722265.1	putative dihydrolipoamide dehydrogenase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GCVL-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCVL-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb07169	XM_003722217.1 Latest version!	Leishmania major strain Friedlin putative dihydrolipoamide dehydrogenase (GCVL-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCVL-1 ( XM_003722217.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_003722265.1 Leishmania major strain Friedlin putative dihydrolipoamide dehydrogenase (GCVL-1), partial mRNA.

Price & Availability↑

CloneID	OLb07169
Clone ID Related Accession (Same CDS sequence)	XM_003722217.1
Accession Version	XM_003722217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	putative dihydrolipoamide dehydrogenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007270). COMPLETENESS: incomplete on both ends.

ATGAAGCGCA CTATCGTTGC ATGGACGAGG AAATTCGATG TGTGCGTGCT AGGCGGTGGT 
CCAGCCGGCA TCGCCGCGGC CGTCCGCGCG TACGAACTGG GGAAAAAGGC CTGCATCATT 
GAAGAGTCCC GTATTGGTGG TGCTGATTTT TGGAATGGTG CGCTGCAGTC CAAGACATTG 
TGGGAAATGG CGAAATTCGC GCGATACACA ATGGGCAACA CGTCTCACCG TTTCATGAAG 
TCCGTAATCG AGCTTCCGAA GATAAAACAC AGCAACCTAA TCAAGGCCAT CACAAACGCC 
GCCGAAACGC GTGAAACTCA GACGCTGGAA GTCCTCGCTA ACGCCCACAT CGAGGTGCTT 
TCCGGATTAG GTTCCTTTAA GACACCGAAC TCGGTAGCTG TCACGAAGAA AGACGGCACG 
GAGGAAACTG TCGAGGCTGA TTACTTTGTG ATTGCTACAG GTGCCCACCC ACGGCCTCAC 
CCAACTGCCG TCGCCGACGG AAAGGTTGTC TTTACCTCCG ATGATATAAT GATGCAACCG 
CTTCCCAAGA GTATTGTTAT TATCGGCGCG GGTGTCATCG GATGTGAGTT TGCATCCATT 
TTCGCCAATT TCGGAGTAAC CCAAGTGAAT ATTATTGAAA AAAGTGGCCG TATTTTGCCT 
ATGGAGGATG AAGATATCGC ACTCTTCGTA CAAACACTTC TTGAGCAAAA AGGCGTTTGT 
TTTCATCATC ACTCCGCGAT GGAGAGCAGT AGCATCAATG ATGGAAAGTT CCACTACACT 
CTCAGAGATG TTCGTGACAA CTCTCTTCAC CAACATGTCA CGGACAGCGC TCTCGTCTCC 
ATCGGACGCG TACCAGCAAT TTCAAAGCTG AACTTGGAGG CAATTGGCAT CACGGTGAAA 
GGTAACCGCA TCGACCGAGA CGAATTCCTT CGCATCGAAC CACACAAGCA CATCTATGCC 
TGTGGAGATA CGTGCACGCG TGTCGCTCTC GTCAACGTTG CCGAACTGGA GGGGCGCGCG 
TGCATTGACC ATATGTACAC ACCATATCCT GAGGAGCAAT TGAAGCTCAA GCTGGACAAT 
CTCTCCACCA TCATGTTCCT TGATCAAGAA GTGGCAGCTG TCGGCCTAAA CGAGCAGCAG 
TGCCAAAAGA TGTCCATCAG CTACAAGATG GCACGATACA GCTACGAATA CGTAGGGCGT 
GCATTAGCTA TGGGCAACAC GAGAGGTTTT ATCAAACTAG TTGTGACAAA CGACAAAAAG 
ATGCAAGTTC TTGGCGTGCG CGCAGTGGGT CCCCATGCCA GCAGTATTAT TGAGCTCGCG 
TCACTGGCCA TCCACAACCG CGACTCTGCA TACGACTTGC GCAATCTTCA CGCCGTCTAC 
CCCGCTATCA CTCAGGGATT CCAAGAGTGC CTTCATATGC TTTTGGGCTC CTCGATTTTG 
AAACCGGACG TCTTCCCGAA GCTTGTTGTG CGAGAATGGA ATCCACCTCA TTTCCAGCAC 
GGGCGCGCAT ACGCGCATAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003722265.1
CDS	1..1527
Translation	MKRTIVAWTRKFDVCVLGGGPAGIAAAVRAYELGKKACIIEESRIGGADFWNGALQSKTLWEMAKFARYTMGNTSHRFMKSVIELPKIKHSNLIKAITNAAETRETQTLEVLANAHIEVLSGLGSFKTPNSVAVTKKDGTEETVEADYFVIATGAHPRPHPTAVADGKVVFTSDDIMMQPLPKSIVIIGAGVIGCEFASIFANFGVTQVNIIEKSGRILPMEDEDIALFVQTLLEQKGVCFHHHSAMESSSINDGKFHYTLRDVRDNSLHQHVTDSALVSIGRVPAISKLNLEAIGITVKGNRIDRDEFLRIEPHKHIYACGDTCTRVALVNVAELEGRACIDHMYTPYPEEQLKLKLDNLSTIMFLDQEVAAVGLNEQQCQKMSISYKMARYSYEYVGRALAMGNTRGFIKLVVTNDKKMQVLGVRAVGPHASSIIELASLAIHNRDSAYDLRNLHAVYPAITQGFQECLHMLLGSSILKPDVFPKLVVREWNPPHFQHGRAYAHTK

Target ORF information:

RefSeq Version	XM_003722217.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin putative dihydrolipoamide dehydrogenase (GCVL-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003722217.1

ORF Insert Sequence:

ATGAAGCGCA CTATCGTTGC ATGGACGAGG AAATTCGATG TGTGCGTGCT AGGCGGTGGT 
CCAGCCGGCA TCGCCGCGGC CGTCCGCGCG TACGAACTGG GGAAAAAGGC CTGCATCATT 
GAAGAGTCCC GTATTGGTGG TGCTGATTTT TGGAATGGTG CGCTGCAGTC CAAGACATTG 
TGGGAAATGG CGAAATTCGC GCGATACACA ATGGGCAACA CGTCTCACCG TTTCATGAAG 
TCCGTAATCG AGCTTCCGAA GATAAAACAC AGCAACCTAA TCAAGGCCAT CACAAACGCC 
GCCGAAACGC GTGAAACTCA GACGCTGGAA GTCCTCGCTA ACGCCCACAT CGAGGTGCTT 
TCCGGATTAG GTTCCTTTAA GACACCGAAC TCGGTAGCTG TCACGAAGAA AGACGGCACG 
GAGGAAACTG TCGAGGCTGA TTACTTTGTG ATTGCTACAG GTGCCCACCC ACGGCCTCAC 
CCAACTGCCG TCGCCGACGG AAAGGTTGTC TTTACCTCCG ATGATATAAT GATGCAACCG 
CTTCCCAAGA GTATTGTTAT TATCGGCGCG GGTGTCATCG GATGTGAGTT TGCATCCATT 
TTCGCCAATT TCGGAGTAAC CCAAGTGAAT ATTATTGAAA AAAGTGGCCG TATTTTGCCT 
ATGGAGGATG AAGATATCGC ACTCTTCGTA CAAACACTTC TTGAGCAAAA AGGCGTTTGT 
TTTCATCATC ACTCCGCGAT GGAGAGCAGT AGCATCAATG ATGGAAAGTT CCACTACACT 
CTCAGAGATG TTCGTGACAA CTCTCTTCAC CAACATGTCA CGGACAGCGC TCTCGTCTCC 
ATCGGACGCG TACCAGCAAT TTCAAAGCTG AACTTGGAGG CAATTGGCAT CACGGTGAAA 
GGTAACCGCA TCGACCGAGA CGAATTCCTT CGCATCGAAC CACACAAGCA CATCTATGCC 
TGTGGAGATA CGTGCACGCG TGTCGCTCTC GTCAACGTTG CCGAACTGGA GGGGCGCGCG 
TGCATTGACC ATATGTACAC ACCATATCCT GAGGAGCAAT TGAAGCTCAA GCTGGACAAT 
CTCTCCACCA TCATGTTCCT TGATCAAGAA GTGGCAGCTG TCGGCCTAAA CGAGCAGCAG 
TGCCAAAAGA TGTCCATCAG CTACAAGATG GCACGATACA GCTACGAATA CGTAGGGCGT 
GCATTAGCTA TGGGCAACAC GAGAGGTTTT ATCAAACTAG TTGTGACAAA CGACAAAAAG 
ATGCAAGTTC TTGGCGTGCG CGCAGTGGGT CCCCATGCCA GCAGTATTAT TGAGCTCGCG 
TCACTGGCCA TCCACAACCG CGACTCTGCA TACGACTTGC GCAATCTTCA CGCCGTCTAC 
CCCGCTATCA CTCAGGGATT CCAAGAGTGC CTTCATATGC TTTTGGGCTC CTCGATTTTG 
AAACCGGACG TCTTCCCGAA GCTTGTTGTG CGAGAATGGA ATCCACCTCA TTTCCAGCAC 
GGGCGCGCAT ACGCGCATAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

GCVL-1 cDNA ORF clone, Leishmania major strain Friedlin

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM