LINJ_25_0630 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_25_0630
Entrez Gene ID	5069527
Full Name	putative RNA polymerase I second largest subunit
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001466057.1	XP_001466094.1	putative RNA polymerase I second largest subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_25_0630 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_25_0630 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc03163	XM_001466057.1 Latest version!	Leishmania infantum JPCM5 putative RNA polymerase I second largest subunit partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_25_0630 ( XM_001466057.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001466094.1 Leishmania infantum JPCM5 putative RNA polymerase I second largest subunit partial mRNA.

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CloneID	OLc03163
Clone ID Related Accession (Same CDS sequence)	XM_001466057.1
Accession Version	XM_001466057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative RNA polymerase I second largest subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009409). COMPLETENESS: incomplete on both ends.

ATGTCGTCTC GCTCATCACA CTATAGCGCA AAGTCGCTCT CCTCCTCAGC GGGGGAGTCG 
ATGGTAACGA GCCACAGTAC TGGGTCCTCA AGCCGTCACC GTCGCACCGG CACTGCCTGC 
ATACATGAGC CACGCGCTCC ACCCATAAAG ATGACGGCGA TGGAGCGCCT GCAACACATC 
CGCAACTCCA TTACTCGGCT GAAGTACATT TGTGAACGGC AGTATCACAC CGCCGCGCTC 
GATCAGGCGT CCGAGGCGTC CGTCACGCCG TTCTTTGAGC GGCTCCGCAA GAAGCTGTCG 
AAGCTCGAGT TCAGCCGTAG CCACATCGGC ACCCCAGAGA CCGGCGGCCC GCAGCAGCTG 
AAGTACATGG CGAAAGAATC GAGGCAGCTC ATGAGCACTG CGCAGGAGGT GGTGCGCGAC 
ACCGCGAACA CTTCCGCGAC GGCGACATGT GTTTACGAGG GTATGCAGCA TCATCACATG 
CAAGAGGCCA TGGCGGAGCT GGAGGAGCTG TCCGCGCTCC TGGCAGAGCG CCCCAACGAG 
CAGCTGAGTC GCGTGAACGC CGATCTTCTG GCGGACCTCG TTGCTTTCCA CATCGACGAG 
TTCGACGCCT TTATGTCGCT GCGTGTCGAA GCGTTTGCGA GGGAAATGCA GAGCGAAGGC 
GCCTTCTACA CCGGCCCCGT CATGACGGAT GCGTCTCAGA AGGCTACGCC AGTCGGTGTG 
TGCTCGGCGG TGCTGCTAGA CGTTGCGGTC GGCGTCCGGC CAGCGCGGTC GGCTCGCATC 
GTGCTTGAGC ATGCACGGCA TCAGATCAAC AAAGTCGAGA ACCGTCTGCA CGCTGCGGAC 
ATCGCGCTGC AGGAGTGCCA GCGGGCGGGC GAGGTTGCAG CAGATGGCCA GCAAAGGCAG 
CAGGCGGAGG TGCAGCGTCG CGAGTTCACC ATCATGAAGG AGCGGATCAG CCACGCCCTG 
CGCCAGCTGG ATCGCGCTCA GCAAGCATGG CTGGAAGAGG AGCAGAGCAT CTTCACTCGT 
GCCGCCTCCG GTAGCCACGA TCTGGCGGCG CTGATGCAGA CAAAGCACCT GCGCGAGGCG 
GAGGTGATCG AGGAGGTGCA GCGGCTGCAC GACCAGACGA TCCACGCTTG CCTGCCGTTC 
GCCACTCCGC AGTATCACCG CCGCTACGGT GAGACGTACG GCCAGCCGTG CCACGTCCGC 
CTCGGCTTCC ACTTCCTGCC CGATGCGAAC ACGGCAGCCG AGATGGCCGC CGCTGCTGGA 
TCGGCCAATG CCGCGGCGCC GTCGTCGCTG ATGCTGCGCA CCTTCTCCAC GGAGTACTTT 
GCGTTGATGC GCCAGCTGCG CGTCGCTCCT GCCGACTACG ACACCTACGA CTACCTTGTG 
CCGAGCAAGG AGCAGTTCTG GGTGAACTTT GCGAGCTTCC CGGAGATGGT GAAAGGGGCC 
CGCTGCGCCC TTCGCAACGT CGACGCTCGC CTCGACCACT TCCGCGCGCT GGAGGAGCAG 
AAGGAGGTAG GCGGCTACTT CATCATGAAC GGCGGCGAGC GCATTCTGCG TGCTTTGCTC 
ATGCAGCGCT GCAACGTGCC GATCAACATC TACCGCGAGA AGTTCGTCAC GCAGGGCCCG 
CACTTTTCCG CCAAGGCTGT CGTCATTCGC TGCAAGCGGC CAAGCGGGCT GACGGCGCAG 
AACTACTTCT ACTACACAAC GCAGGGCGAG GTGATCTTCT CATTCGCGCG CAAGGTGGTG 
TGGCATCTAC CGGTGCCGCT GCTGCTGAGC GCAACGAGTA CGCAGAGCGT GTCAGCGGTG 
GAGCTGCAGC GGCTGCTGAC GATCGGCATG CCAGATGGCA ACCACGCAGC CCGTGTTGAG 
GCGCTCCTCC AACACCACCG CCGCAAGCCC TACGGTGATC TGCGGCACTT TATCGACTAC 
ATCACGGTTC TTGGCCGCAT GTACCGCGAG TACCACCAGA ACTCATCTAC GTTTCACTTT 
CTGCCGCTCT ACGCGAAGGG CTCGACGTGC CAGCATGACG CGTGGTACGG CATGTTCATG 
CTGCGACGCC ACGTCTTGCC GCACTTGAAC AGCTGTGATG CGGCCACGCC GGATCTGGCC 
CCTGACGCCA CTGCGCAGCA GCTGCTAGCG TGGCTGTCAC CCGCTCTTCT CGCTGAGCTG 
GACCGCAAGT TTGACGCGAT GATCGCCATC ACGCGGCAGC TGTACCTCTT CATCGACGGC 
GCTGTCGAGC ACCAGGGCAA CGACGTCCCG GCTTATCAGG AGCTCTTCAC CGTCTCGCAG 
GTGCTCATGG GTGCCTTTGA GGTGTGCCTG AACCGGTACA TGCGCGGCTT TGTCTACCGC 
TTCGCACGTC ACCTGCCCGC GGCGCTCTTT CGCCGCATTC TCCACCTGCG CACTCTGCTG 
CCCCCCGTCG ACGCCGAGGA CGCCATGCGG CAGGTGCGTC AGTTTGCGGA ATTTTGTGGC 
CGGCACGGCG GCAGCGACCC GCTGGATCAG CTTGCCCGTC TGCTCGTGAC GGGCAATATC 
AACCTCAACC GCGAGGAGGA CTTTTACTGC CCGCAGACGT CTGGGTGGGT GGTCATGGCC 
GAGCACCTGA ACTTCTACCG CTTCTTTGAG CAGCTTCGCT GCGTGCACCG TGGCAAGACT 
ATCGCCGACA TGCGCTCGAG TGAGGTGCGC AAGTACCCGT GTGAAGCGTA CGGCTTTATC 
TGCATGGTGC AAAGCCCCGA CGGCGAGGAC TGTGGGGTGC TGAATCACCT GAGTTTGTCG 
ACCATCTCGT CCAACTCACC GGATGCGGCC ATGACAGCGC AGCTGCGGGA GATAGTGTGC 
AAGGCGGTGC CGGGCGTGCG CAGCCGCGCG ACGCACAGCA CGGTGGTTGA TCAGCTTTGT 
GAGACAGTGC CGGTGTGGAT GGAGGGCGAG CTGCTTGGCT ACCTCAGCCC GGCGGACGCT 
GTGGAGGCCG CGGCAAGGCT GCGCCAGCGC AAGGCGCTCA CGCTGCGCTC GCAGGTGCAC 
GTGACCGGCA TCGTACGCCG AAAGGATGCG TCACCTCTGC ACACGCTGGA GGTGGTGTAC 
GTGGCGCCGA AGAACAAGGA TCCGGCGGGC CTGTACGTCT TCTACGACTG CGGACGACTC 
ATGCGCCCAG TGCAGCGGCT CGAGTCGTCG GTGCGCAAGG ATGCAACCCA TCTCCCTTTC 
CCGCTCGTGT TCATTGGCAC CTGGGAGCAG AGCTGGCTGG ATATCGCCGC CGTCCCGAGC 
GACCCCCTCG ACGCCGTCGT GCAGCTGAAC CGCAAGTATG AGTACATGGA GCAGAACGGC 
ACCAACCTCA TCTCCCTCAC CTCGGCGACC ATCCCCTTCT TCGAGCACAA CTGCTCGCCG 
CGCAACCTGT TCCAGTGCGG CCTCAGCAAG CAGTCCTCTG GCACACAGCT GCAGGCGCTG 
GCGTGGCGCA AGGAGGCAAA GCTGTTCCGT ACCTATTGCC CGCAGCGCTA CATCAGCCGC 
ACCCTCCCCA TGGACTACTA CGGCCTCGAC GACGTGAACC TCGGCGTCAA TGCCGTGATT 
GCCATTCTCG CCTACACCGG CTACGACATG GACGACGCCG TCATCATCAA CAGCACCGCT 
GCCCAGCGCG GTATGCTGAC GGCCGGTGTC ACGGTCGCCA AGATTGTGAC TGCATCCGGC 
AAGGGCTCTG ACAAGGACGA CGTCTTCGTG TTCCACAACC TTCTCTCCAC GGGCGAGCGA 
TTCACCGCCG AGCTGGAGGC AAACGGGCTA CCGCCGAAGC GCGCGAACGC GAGCCTGGAC 
GCGTTCTCCT TCGATCGCGA TCACAAGTAC CCTGGACTGC GAGACAACAA CGACGTGTAC 
TGCTGCGCCA AGCGGGTGGA GCGGGTGGAC CCGTTCACGA ACAAGTCTGT CTATGAGTAC 
ACGCGCCACC ACGCCACCAA GTGGCGCCAC TTCGACAAGG GCGAGGATGC GTGGGTCCAG 
CAGGTGATCC CGCTCGTCTA CAGCGGGCCA GACCCGACAT CGGTGCTCAT GATCTTCCGC 
ATCCCGCGCC CGCCGACCGT GGGCGACAAG TTCTCGTCCC GCCACGGCCA GAAGGGTACG 
CTGCCGCTAC ACATTCGTTC TCACAACCTA CCCTTCGCCA CGGCCAGCGG CATAACGCCG 
GACGTCATCA TCAACCCCCA CGCCTTCCCG TCGCGCATGA CGGTCGGCAT GGTGCTGGAG 
ATCATGACCG CCAAGGTCGG TGCCATCGAG GGTCGCTTCA TCGATCACAG TGCGTGGTCG 
ACCGTAGACG AGCAGCCGCG AGTGGCGGAG TTGATTGGCG AAGCGCTGGT CAAGGCAGGG 
TACAACCGCT ACGGCCGCGA GCACCTGATC GATGGCATCA GCGGTGAAGA GATGAAGGCG 
GACGTGTTCA TGGGTATCTG CGGGTATCAG CGTCTGCGTC ACATGGTTAG TGACAAGTGG 
CAGGCGCGGG CGCGTACGGA CGCGCACACA TACCGGGCGG TAACCAAGAC GGGGCAGCCG 
GTGAAGGGCC GCAAGCGCCA CGGTGGCGTG CGGGTTGGGG AGATGGAGCG GGACGGCTTG 
CTATCGCACG GCATTAGTGA AGTTGTTGTA GACCGTCTCC TGCACGTGTC AGACAAGACA 
AAGGCGTTCA TCTGCCCGAG GTGCGGTAGC TTGCTCTCCC TTTACGAGCG CCATGCCACT 
GAGTACGGTA CATGGCGCAC CTGTCGCTTC TGCGGCGCCG GCGCGGACGA AAGCACCGAC 
TCGATCGCTA TGGTGGAGAT TCCACAAGTG CTCCGGCTGT GGGCGGCGGA GCTGACGAGC 
ATTGGCGTCC GCGTCGCGCT CAAGACGTCG GAGCTGTCCG ACTTTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001466094.1
CDS	1..4851
Translation	MSSRSSHYSAKSLSSSAGESMVTSHSTGSSSRHRRTGTACIHEPRAPPIKMTAMERLQHIRNSITRLKYICERQYHTAALDQASEASVTPFFERLRKKLSKLEFSRSHIGTPETGGPQQLKYMAKESRQLMSTAQEVVRDTANTSATATCVYEGMQHHHMQEAMAELEELSALLAERPNEQLSRVNADLLADLVAFHIDEFDAFMSLRVEAFAREMQSEGAFYTGPVMTDASQKATPVGVCSAVLLDVAVGVRPARSARIVLEHARHQINKVENRLHAADIALQECQRAGEVAADGQQRQQAEVQRREFTIMKERISHALRQLDRAQQAWLEEEQSIFTRAASGSHDLAALMQTKHLREAEVIEEVQRLHDQTIHACLPFATPQYHRRYGETYGQPCHVRLGFHFLPDANTAAEMAAAAGSANAAAPSSLMLRTFSTEYFALMRQLRVAPADYDTYDYLVPSKEQFWVNFASFPEMVKGARCALRNVDARLDHFRALEEQKEVGGYFIMNGGERILRALLMQRCNVPINIYREKFVTQGPHFSAKAVVIRCKRPSGLTAQNYFYYTTQGEVIFSFARKVVWHLPVPLLLSATSTQSVSAVELQRLLTIGMPDGNHAARVEALLQHHRRKPYGDLRHFIDYITVLGRMYREYHQNSSTFHFLPLYAKGSTCQHDAWYGMFMLRRHVLPHLNSCDAATPDLAPDATAQQLLAWLSPALLAELDRKFDAMIAITRQLYLFIDGAVEHQGNDVPAYQELFTVSQVLMGAFEVCLNRYMRGFVYRFARHLPAALFRRILHLRTLLPPVDAEDAMRQVRQFAEFCGRHGGSDPLDQLARLLVTGNINLNREEDFYCPQTSGWVVMAEHLNFYRFFEQLRCVHRGKTIADMRSSEVRKYPCEAYGFICMVQSPDGEDCGVLNHLSLSTISSNSPDAAMTAQLREIVCKAVPGVRSRATHSTVVDQLCETVPVWMEGELLGYLSPADAVEAAARLRQRKALTLRSQVHVTGIVRRKDASPLHTLEVVYVAPKNKDPAGLYVFYDCGRLMRPVQRLESSVRKDATHLPFPLVFIGTWEQSWLDIAAVPSDPLDAVVQLNRKYEYMEQNGTNLISLTSATIPFFEHNCSPRNLFQCGLSKQSSGTQLQALAWRKEAKLFRTYCPQRYISRTLPMDYYGLDDVNLGVNAVIAILAYTGYDMDDAVIINSTAAQRGMLTAGVTVAKIVTASGKGSDKDDVFVFHNLLSTGERFTAELEANGLPPKRANASLDAFSFDRDHKYPGLRDNNDVYCCAKRVERVDPFTNKSVYEYTRHHATKWRHFDKGEDAWVQQVIPLVYSGPDPTSVLMIFRIPRPPTVGDKFSSRHGQKGTLPLHIRSHNLPFATASGITPDVIINPHAFPSRMTVGMVLEIMTAKVGAIEGRFIDHSAWSTVDEQPRVAELIGEALVKAGYNRYGREHLIDGISGEEMKADVFMGICGYQRLRHMVSDKWQARARTDAHTYRAVTKTGQPVKGRKRHGGVRVGEMERDGLLSHGISEVVVDRLLHVSDKTKAFICPRCGSLLSLYERHATEYGTWRTCRFCGAGADESTDSIAMVEIPQVLRLWAAELTSIGVRVALKTSELSDFI

Target ORF information:

RefSeq Version	XM_001466057.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative RNA polymerase I second largest subunit partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001466057.1

ORF Insert Sequence:

ATGTCGTCTC GCTCATCACA CTATAGCGCA AAGTCGCTCT CCTCCTCAGC GGGGGAGTCG 
ATGGTAACGA GCCACAGTAC TGGGTCCTCA AGCCGTCACC GTCGCACCGG CACTGCCTGC 
ATACATGAGC CACGCGCTCC ACCCATAAAG ATGACGGCGA TGGAGCGCCT GCAACACATC 
CGCAACTCCA TTACTCGGCT GAAGTACATT TGTGAACGGC AGTATCACAC CGCCGCGCTC 
GATCAGGCGT CCGAGGCGTC CGTCACGCCG TTCTTTGAGC GGCTCCGCAA GAAGCTGTCG 
AAGCTCGAGT TCAGCCGTAG CCACATCGGC ACCCCAGAGA CCGGCGGCCC GCAGCAGCTG 
AAGTACATGG CGAAAGAATC GAGGCAGCTC ATGAGCACTG CGCAGGAGGT GGTGCGCGAC 
ACCGCGAACA CTTCCGCGAC GGCGACATGT GTTTACGAGG GTATGCAGCA TCATCACATG 
CAAGAGGCCA TGGCGGAGCT GGAGGAGCTG TCCGCGCTCC TGGCAGAGCG CCCCAACGAG 
CAGCTGAGTC GCGTGAACGC CGATCTTCTG GCGGACCTCG TTGCTTTCCA CATCGACGAG 
TTCGACGCCT TTATGTCGCT GCGTGTCGAA GCGTTTGCGA GGGAAATGCA GAGCGAAGGC 
GCCTTCTACA CCGGCCCCGT CATGACGGAT GCGTCTCAGA AGGCTACGCC AGTCGGTGTG 
TGCTCGGCGG TGCTGCTAGA CGTTGCGGTC GGCGTCCGGC CAGCGCGGTC GGCTCGCATC 
GTGCTTGAGC ATGCACGGCA TCAGATCAAC AAAGTCGAGA ACCGTCTGCA CGCTGCGGAC 
ATCGCGCTGC AGGAGTGCCA GCGGGCGGGC GAGGTTGCAG CAGATGGCCA GCAAAGGCAG 
CAGGCGGAGG TGCAGCGTCG CGAGTTCACC ATCATGAAGG AGCGGATCAG CCACGCCCTG 
CGCCAGCTGG ATCGCGCTCA GCAAGCATGG CTGGAAGAGG AGCAGAGCAT CTTCACTCGT 
GCCGCCTCCG GTAGCCACGA TCTGGCGGCG CTGATGCAGA CAAAGCACCT GCGCGAGGCG 
GAGGTGATCG AGGAGGTGCA GCGGCTGCAC GACCAGACGA TCCACGCTTG CCTGCCGTTC 
GCCACTCCGC AGTATCACCG CCGCTACGGT GAGACGTACG GCCAGCCGTG CCACGTCCGC 
CTCGGCTTCC ACTTCCTGCC CGATGCGAAC ACGGCAGCCG AGATGGCCGC CGCTGCTGGA 
TCGGCCAATG CCGCGGCGCC GTCGTCGCTG ATGCTGCGCA CCTTCTCCAC GGAGTACTTT 
GCGTTGATGC GCCAGCTGCG CGTCGCTCCT GCCGACTACG ACACCTACGA CTACCTTGTG 
CCGAGCAAGG AGCAGTTCTG GGTGAACTTT GCGAGCTTCC CGGAGATGGT GAAAGGGGCC 
CGCTGCGCCC TTCGCAACGT CGACGCTCGC CTCGACCACT TCCGCGCGCT GGAGGAGCAG 
AAGGAGGTAG GCGGCTACTT CATCATGAAC GGCGGCGAGC GCATTCTGCG TGCTTTGCTC 
ATGCAGCGCT GCAACGTGCC GATCAACATC TACCGCGAGA AGTTCGTCAC GCAGGGCCCG 
CACTTTTCCG CCAAGGCTGT CGTCATTCGC TGCAAGCGGC CAAGCGGGCT GACGGCGCAG 
AACTACTTCT ACTACACAAC GCAGGGCGAG GTGATCTTCT CATTCGCGCG CAAGGTGGTG 
TGGCATCTAC CGGTGCCGCT GCTGCTGAGC GCAACGAGTA CGCAGAGCGT GTCAGCGGTG 
GAGCTGCAGC GGCTGCTGAC GATCGGCATG CCAGATGGCA ACCACGCAGC CCGTGTTGAG 
GCGCTCCTCC AACACCACCG CCGCAAGCCC TACGGTGATC TGCGGCACTT TATCGACTAC 
ATCACGGTTC TTGGCCGCAT GTACCGCGAG TACCACCAGA ACTCATCTAC GTTTCACTTT 
CTGCCGCTCT ACGCGAAGGG CTCGACGTGC CAGCATGACG CGTGGTACGG CATGTTCATG 
CTGCGACGCC ACGTCTTGCC GCACTTGAAC AGCTGTGATG CGGCCACGCC GGATCTGGCC 
CCTGACGCCA CTGCGCAGCA GCTGCTAGCG TGGCTGTCAC CCGCTCTTCT CGCTGAGCTG 
GACCGCAAGT TTGACGCGAT GATCGCCATC ACGCGGCAGC TGTACCTCTT CATCGACGGC 
GCTGTCGAGC ACCAGGGCAA CGACGTCCCG GCTTATCAGG AGCTCTTCAC CGTCTCGCAG 
GTGCTCATGG GTGCCTTTGA GGTGTGCCTG AACCGGTACA TGCGCGGCTT TGTCTACCGC 
TTCGCACGTC ACCTGCCCGC GGCGCTCTTT CGCCGCATTC TCCACCTGCG CACTCTGCTG 
CCCCCCGTCG ACGCCGAGGA CGCCATGCGG CAGGTGCGTC AGTTTGCGGA ATTTTGTGGC 
CGGCACGGCG GCAGCGACCC GCTGGATCAG CTTGCCCGTC TGCTCGTGAC GGGCAATATC 
AACCTCAACC GCGAGGAGGA CTTTTACTGC CCGCAGACGT CTGGGTGGGT GGTCATGGCC 
GAGCACCTGA ACTTCTACCG CTTCTTTGAG CAGCTTCGCT GCGTGCACCG TGGCAAGACT 
ATCGCCGACA TGCGCTCGAG TGAGGTGCGC AAGTACCCGT GTGAAGCGTA CGGCTTTATC 
TGCATGGTGC AAAGCCCCGA CGGCGAGGAC TGTGGGGTGC TGAATCACCT GAGTTTGTCG 
ACCATCTCGT CCAACTCACC GGATGCGGCC ATGACAGCGC AGCTGCGGGA GATAGTGTGC 
AAGGCGGTGC CGGGCGTGCG CAGCCGCGCG ACGCACAGCA CGGTGGTTGA TCAGCTTTGT 
GAGACAGTGC CGGTGTGGAT GGAGGGCGAG CTGCTTGGCT ACCTCAGCCC GGCGGACGCT 
GTGGAGGCCG CGGCAAGGCT GCGCCAGCGC AAGGCGCTCA CGCTGCGCTC GCAGGTGCAC 
GTGACCGGCA TCGTACGCCG AAAGGATGCG TCACCTCTGC ACACGCTGGA GGTGGTGTAC 
GTGGCGCCGA AGAACAAGGA TCCGGCGGGC CTGTACGTCT TCTACGACTG CGGACGACTC 
ATGCGCCCAG TGCAGCGGCT CGAGTCGTCG GTGCGCAAGG ATGCAACCCA TCTCCCTTTC 
CCGCTCGTGT TCATTGGCAC CTGGGAGCAG AGCTGGCTGG ATATCGCCGC CGTCCCGAGC 
GACCCCCTCG ACGCCGTCGT GCAGCTGAAC CGCAAGTATG AGTACATGGA GCAGAACGGC 
ACCAACCTCA TCTCCCTCAC CTCGGCGACC ATCCCCTTCT TCGAGCACAA CTGCTCGCCG 
CGCAACCTGT TCCAGTGCGG CCTCAGCAAG CAGTCCTCTG GCACACAGCT GCAGGCGCTG 
GCGTGGCGCA AGGAGGCAAA GCTGTTCCGT ACCTATTGCC CGCAGCGCTA CATCAGCCGC 
ACCCTCCCCA TGGACTACTA CGGCCTCGAC GACGTGAACC TCGGCGTCAA TGCCGTGATT 
GCCATTCTCG CCTACACCGG CTACGACATG GACGACGCCG TCATCATCAA CAGCACCGCT 
GCCCAGCGCG GTATGCTGAC GGCCGGTGTC ACGGTCGCCA AGATTGTGAC TGCATCCGGC 
AAGGGCTCTG ACAAGGACGA CGTCTTCGTG TTCCACAACC TTCTCTCCAC GGGCGAGCGA 
TTCACCGCCG AGCTGGAGGC AAACGGGCTA CCGCCGAAGC GCGCGAACGC GAGCCTGGAC 
GCGTTCTCCT TCGATCGCGA TCACAAGTAC CCTGGACTGC GAGACAACAA CGACGTGTAC 
TGCTGCGCCA AGCGGGTGGA GCGGGTGGAC CCGTTCACGA ACAAGTCTGT CTATGAGTAC 
ACGCGCCACC ACGCCACCAA GTGGCGCCAC TTCGACAAGG GCGAGGATGC GTGGGTCCAG 
CAGGTGATCC CGCTCGTCTA CAGCGGGCCA GACCCGACAT CGGTGCTCAT GATCTTCCGC 
ATCCCGCGCC CGCCGACCGT GGGCGACAAG TTCTCGTCCC GCCACGGCCA GAAGGGTACG 
CTGCCGCTAC ACATTCGTTC TCACAACCTA CCCTTCGCCA CGGCCAGCGG CATAACGCCG 
GACGTCATCA TCAACCCCCA CGCCTTCCCG TCGCGCATGA CGGTCGGCAT GGTGCTGGAG 
ATCATGACCG CCAAGGTCGG TGCCATCGAG GGTCGCTTCA TCGATCACAG TGCGTGGTCG 
ACCGTAGACG AGCAGCCGCG AGTGGCGGAG TTGATTGGCG AAGCGCTGGT CAAGGCAGGG 
TACAACCGCT ACGGCCGCGA GCACCTGATC GATGGCATCA GCGGTGAAGA GATGAAGGCG 
GACGTGTTCA TGGGTATCTG CGGGTATCAG CGTCTGCGTC ACATGGTTAG TGACAAGTGG 
CAGGCGCGGG CGCGTACGGA CGCGCACACA TACCGGGCGG TAACCAAGAC GGGGCAGCCG 
GTGAAGGGCC GCAAGCGCCA CGGTGGCGTG CGGGTTGGGG AGATGGAGCG GGACGGCTTG 
CTATCGCACG GCATTAGTGA AGTTGTTGTA GACCGTCTCC TGCACGTGTC AGACAAGACA 
AAGGCGTTCA TCTGCCCGAG GTGCGGTAGC TTGCTCTCCC TTTACGAGCG CCATGCCACT 
GAGTACGGTA CATGGCGCAC CTGTCGCTTC TGCGGCGCCG GCGCGGACGA AAGCACCGAC 
TCGATCGCTA TGGTGGAGAT TCCACAAGTG CTCCGGCTGT GGGCGGCGGA GCTGACGAGC 
ATTGGCGTCC GCGTCGCGCT CAAGACGTCG GAGCTGTCCG ACTTTATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_25_0630 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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