LINJ_24_0780 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_24_0780
Entrez Gene ID	5069333
Full Name	malic enzyme
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001465869.1	XP_001465906.1	malic enzyme

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_24_0780 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_24_0780 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc02980	XM_001465869.1 Latest version!	Leishmania infantum JPCM5 malic enzyme partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_24_0780 ( XM_001465869.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001465906.1 Leishmania infantum JPCM5 malic enzyme partial mRNA.

Price & Availability↑

CloneID	OLc02980
Clone ID Related Accession (Same CDS sequence)	XM_001465869.1
Accession Version	XM_001465869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	malic enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009408). COMPLETENESS: incomplete on both ends.

ATGTCCCTCC AAAACGATCT GCAGAGCAAG ACGGCGTCGC ATGTGCACTC GCACTGTGCA 
CATGTCCTGA CGAACCGCTA CCTGAACCGC GGCACCGCCT TTACGACAGA GCAGCGTAAG 
AAGCTTGGCA TTCTTGGTGC CCTGCCGCCC ACCGTCGAGA CCCTCGAGAT GCAGATTGAG 
CGTGCCTGGA TGCAGCTGTG CCGCTACGAC AAGCCCATCA ACCGCTACCA CCACCTGGTC 
AGCATCCATG CCACGAACAC GACGCTTTAC TACGCCCTCG TGCTCACCCA CATCGAGGAA 
CTGCTCCCCA TCATCTACAC GCCGACCGTC GGCGAGGCCT GCCAGAACTT CAGCAACTAC 
TGGGTGCGTG AGCGCGGAAT GTATTTGAGC AAGTACCTCA AGGGTCACTT CTCTGAGGTG 
ATGAAAGAGT CTCTGTACGA CAACGTCGAC GTCATCGTTA TCACCGATGG CTCCCGCATT 
CTCGGCCTGG GCGACCTCGG CGCCAACGGC ATCGGCATCA GCATCGGCAA GTGCTCCCTG 
TACGTCGCCG GCGCCGGCCT GCATCCGAGC CGCGTGCTGC CGGTCGTCAT GGACGTTGGC 
ACAAACACGG AACGGTATCT GAACGATCGC GAGTACCTCG GCACCCGCGA GAAGCGCCTC 
GATGACGATC AGTTCTACAG CCTGCTGGAC GAGTTCATGG AAGCTGTGAA GGACACCTGG 
CCCTCCGCTG TCGTGCAGTT CGAGGACTTC AGCAACAACC ACTGCTTCGA CATGCTGGAG 
CGCTACCAAA AGAAGTACCG CTGCTTCAAC GACGATATCC AGGGCACCGG CGCCGTCATA 
GCTGCTGGTT TTCTGAACGC TGTCAAGAAG TCCGGCCTTG GCCCGCTGGA ACAGCGCATC 
GTCGTCTTCG GCGCCGGCTC TGCCGCTGTC GGTGTGGCGA AAAACATCGC CCAGCTGGCC 
TCTCGCATGT ACAACGTGGA CATTGCTGAG GTGTTGAAGA CGTTCTACCT GGTCGACACG 
AAGGGCTTGG TGAGTGACAC CCGTGGTGAC AAGCTGGCGG CGCACAAGGT ACTGCTGTCG 
CGCAAGGACA TCTCGGCCGA GGACAGCCAG AACCTGAAGT CGCTCGAGGA CGTCGTGCAG 
TTCGTGAAGC CGACGGCCCT GCTTGGCCTG GGCGGTGTCG GTCCTGTGTT CACGGAGGCT 
ATAGTGAAGA GCGTCGCCGC CAACGCTGCC CGCCCGATCA TCTTTCCGCT GTCGAATCCC 
ACGAGCAAGT CGGAGGTTAT GCCGGATGAT GCGTACCGCT GGACCAACGG TGCAGCTATT 
ATCGCCTCCG GCAGCCCCTT TCCGGCCTCC ACCATCAACG GCAAGACCAT CAAGCCGTCG 
CAGGGCAACA ACCTGTACGT CTTCCCAGGC GTCGGCCTCG GCTGCGCGCT GGTCCAGCCA 
TCCTACATCC CGGACGACCT CCTGGTGGCT GCCTCGGCGT GCCTGAACCT ACTCACGACC 
CCGGAGCAGA TGGAGGTGGA GCAGCTGTAC CCGCCGCTGG AAGACATCCA TAACATCTCT 
GCCCACATCG CCACCGAGGT TATCATGGAG GCGCAGCGCC TCGGCATCGA CGGAAACAAG 
GACCTGCCGC GCGAGAAGGA TGCCATTCTG GCGGCCATAA AGACATCTCA GTGGGTGCCG 
CACTACCCGG CAGAGCTGGA CGTCAGCCTT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001465906.1
CDS	1..1716
Translation	MSLQNDLQSKTASHVHSHCAHVLTNRYLNRGTAFTTEQRKKLGILGALPPTVETLEMQIERAWMQLCRYDKPINRYHHLVSIHATNTTLYYALVLTHIEELLPIIYTPTVGEACQNFSNYWVRERGMYLSKYLKGHFSEVMKESLYDNVDVIVITDGSRILGLGDLGANGIGISIGKCSLYVAGAGLHPSRVLPVVMDVGTNTERYLNDREYLGTREKRLDDDQFYSLLDEFMEAVKDTWPSAVVQFEDFSNNHCFDMLERYQKKYRCFNDDIQGTGAVIAAGFLNAVKKSGLGPLEQRIVVFGAGSAAVGVAKNIAQLASRMYNVDIAEVLKTFYLVDTKGLVSDTRGDKLAAHKVLLSRKDISAEDSQNLKSLEDVVQFVKPTALLGLGGVGPVFTEAIVKSVAANAARPIIFPLSNPTSKSEVMPDDAYRWTNGAAIIASGSPFPASTINGKTIKPSQGNNLYVFPGVGLGCALVQPSYIPDDLLVAASACLNLLTTPEQMEVEQLYPPLEDIHNISAHIATEVIMEAQRLGIDGNKDLPREKDAILAAIKTSQWVPHYPAELDVSLL

Target ORF information:

RefSeq Version	XM_001465869.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 malic enzyme partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001465869.1

ORF Insert Sequence:

ATGTCCCTCC AAAACGATCT GCAGAGCAAG ACGGCGTCGC ATGTGCACTC GCACTGTGCA 
CATGTCCTGA CGAACCGCTA CCTGAACCGC GGCACCGCCT TTACGACAGA GCAGCGTAAG 
AAGCTTGGCA TTCTTGGTGC CCTGCCGCCC ACCGTCGAGA CCCTCGAGAT GCAGATTGAG 
CGTGCCTGGA TGCAGCTGTG CCGCTACGAC AAGCCCATCA ACCGCTACCA CCACCTGGTC 
AGCATCCATG CCACGAACAC GACGCTTTAC TACGCCCTCG TGCTCACCCA CATCGAGGAA 
CTGCTCCCCA TCATCTACAC GCCGACCGTC GGCGAGGCCT GCCAGAACTT CAGCAACTAC 
TGGGTGCGTG AGCGCGGAAT GTATTTGAGC AAGTACCTCA AGGGTCACTT CTCTGAGGTG 
ATGAAAGAGT CTCTGTACGA CAACGTCGAC GTCATCGTTA TCACCGATGG CTCCCGCATT 
CTCGGCCTGG GCGACCTCGG CGCCAACGGC ATCGGCATCA GCATCGGCAA GTGCTCCCTG 
TACGTCGCCG GCGCCGGCCT GCATCCGAGC CGCGTGCTGC CGGTCGTCAT GGACGTTGGC 
ACAAACACGG AACGGTATCT GAACGATCGC GAGTACCTCG GCACCCGCGA GAAGCGCCTC 
GATGACGATC AGTTCTACAG CCTGCTGGAC GAGTTCATGG AAGCTGTGAA GGACACCTGG 
CCCTCCGCTG TCGTGCAGTT CGAGGACTTC AGCAACAACC ACTGCTTCGA CATGCTGGAG 
CGCTACCAAA AGAAGTACCG CTGCTTCAAC GACGATATCC AGGGCACCGG CGCCGTCATA 
GCTGCTGGTT TTCTGAACGC TGTCAAGAAG TCCGGCCTTG GCCCGCTGGA ACAGCGCATC 
GTCGTCTTCG GCGCCGGCTC TGCCGCTGTC GGTGTGGCGA AAAACATCGC CCAGCTGGCC 
TCTCGCATGT ACAACGTGGA CATTGCTGAG GTGTTGAAGA CGTTCTACCT GGTCGACACG 
AAGGGCTTGG TGAGTGACAC CCGTGGTGAC AAGCTGGCGG CGCACAAGGT ACTGCTGTCG 
CGCAAGGACA TCTCGGCCGA GGACAGCCAG AACCTGAAGT CGCTCGAGGA CGTCGTGCAG 
TTCGTGAAGC CGACGGCCCT GCTTGGCCTG GGCGGTGTCG GTCCTGTGTT CACGGAGGCT 
ATAGTGAAGA GCGTCGCCGC CAACGCTGCC CGCCCGATCA TCTTTCCGCT GTCGAATCCC 
ACGAGCAAGT CGGAGGTTAT GCCGGATGAT GCGTACCGCT GGACCAACGG TGCAGCTATT 
ATCGCCTCCG GCAGCCCCTT TCCGGCCTCC ACCATCAACG GCAAGACCAT CAAGCCGTCG 
CAGGGCAACA ACCTGTACGT CTTCCCAGGC GTCGGCCTCG GCTGCGCGCT GGTCCAGCCA 
TCCTACATCC CGGACGACCT CCTGGTGGCT GCCTCGGCGT GCCTGAACCT ACTCACGACC 
CCGGAGCAGA TGGAGGTGGA GCAGCTGTAC CCGCCGCTGG AAGACATCCA TAACATCTCT 
GCCCACATCG CCACCGAGGT TATCATGGAG GCGCAGCGCC TCGGCATCGA CGGAAACAAG 
GACCTGCCGC GCGAGAAGGA TGCCATTCTG GCGGCCATAA AGACATCTCA GTGGGTGCCG 
CACTACCCGG CAGAGCTGGA CGTCAGCCTT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_24_0780 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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