LINJ_03_0960 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_03_0960
Entrez Gene ID	5066302
Full Name	putative elongation initiation factor 2 alpha subunit
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001462789.1	XP_001462826.1	putative elongation initiation factor 2 alpha subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_03_0960 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_03_0960 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00312	XM_001462789.1 Latest version!	Leishmania infantum JPCM5 putative elongation initiation factor 2 alpha subunit partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_03_0960 ( XM_001462789.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001462826.1 Leishmania infantum JPCM5 putative elongation initiation factor 2 alpha subunit partial mRNA.

Price & Availability↑

CloneID	OLc00312
Clone ID Related Accession (Same CDS sequence)	XM_001462789.1
Accession Version	XM_001462789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative elongation initiation factor 2 alpha subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009388). COMPLETENESS: incomplete on both ends.

ATGGCTTCTT ACTGTGTCAC CGATAGTCCC GAAACTGTGG ACTACAAAAC GAAGTGCTGC 
TGCCGAACGA CGGACAATGT CTACTATGCC GTTCCAAAAG AGTACTTTCG CCTCAATCAG 
AGCCTGGCGC GGCGAAAGCT TCTATTAGCG GAGCCGTTCC CTGTTCCAGT GGACTGCAAA 
ACTCTGGACC ACTTGCTGGT GCTCCTTGAG AAGGCCACAA TTCTCTCCGC TCAGGTTGTG 
GAGGGTGAGA CGAAGGGTAG CAATAACGAG CGTCCGGAGT GGATGCGTGA CTTGAACAAG 
CGTCAGCAGA AGTTTGTTTG CGGGTGCCTG GGCATCACCT CATGGGATGG CAAGGACATT 
CCGTTCTACG TGGAAACGAT GCCCAAGATC AACGACGTCG TTTGGGTGAA AATCACCCAG 
GTGAATGACA CCTCGGCGGT AGTGCAGCTG CTCGAGTACG GCAAGCGCGA AGGCATTATT 
CCGTACACGG AGGTGACTAG GCGCCGTGTG CGCTCGATGG GAAAGCTGAT CAAGGTAGGC 
CGTACCGAGC CGGCGCAGGT GATTCGTATC GACAAGGACA AGGGTTATAT TGACTTGTCA 
AAGAAGCTGG TAACGCCCAA CGAAGCAAAG GCGTGCGAAG CGCACTTCCG CCAGGGGAAC 
GAAGTTCGCT CTATTGTTTG CCATGTCGCT GAGCTGTGCG ATATCCCCGC TATGGATGCA 
ATGGAAATGA TCGCCTATCC GCTTTACCAG CGGGAACCGG GCAAGCACGC GTGGACGTGG 
CTTTACGAAC TGAACCAGAC GGAAGACGTG GAGCGCATTC TGGGTCCGCT GAAGCTTGAC 
AAAGCCATAT CGGACTGCCT GATGTCGACG CTGAAAAACG CGATGCGGCT GAAGGTGTTG 
ACGCTGTTTG CGGAAGTCGA GATCACGTGC TTTGCGTGTG ATGGTGTGGA GGCGATCCGC 
GACGTCCTCA TTCTTGGCCG CAGCTACGGA GAAGGCAGCG ATCCCCAGAT CCACCTGTCC 
GTCAATATCA TCGGACCCCC CAAGTATGGC ATCCGAGCGC GCACGGATAT GAAGGAAGAG 
GGTATTCAGC GAATGAAAGA GGCAATAGAG GCGATGACGG CGGAAATCAA GAAGCGCGGT 
GGCCAGCTGA AGGTGGTGAC GCCTCCGCAG CCTCACGGCG ACGCAGACGA AGGCAAGAAG 
GGTTTGGATG GAGAGGACGA TGATGATGAG GATGCTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001462826.1
CDS	1..1242
Translation	MASYCVTDSPETVDYKTKCCCRTTDNVYYAVPKEYFRLNQSLARRKLLLAEPFPVPVDCKTLDHLLVLLEKATILSAQVVEGETKGSNNERPEWMRDLNKRQQKFVCGCLGITSWDGKDIPFYVETMPKINDVVWVKITQVNDTSAVVQLLEYGKREGIIPYTEVTRRRVRSMGKLIKVGRTEPAQVIRIDKDKGYIDLSKKLVTPNEAKACEAHFRQGNEVRSIVCHVAELCDIPAMDAMEMIAYPLYQREPGKHAWTWLYELNQTEDVERILGPLKLDKAISDCLMSTLKNAMRLKVLTLFAEVEITCFACDGVEAIRDVLILGRSYGEGSDPQIHLSVNIIGPPKYGIRARTDMKEEGIQRMKEAIEAMTAEIKKRGGQLKVVTPPQPHGDADEGKKGLDGEDDDDEDAD

Target ORF information:

RefSeq Version	XM_001462789.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative elongation initiation factor 2 alpha subunit partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001462789.1

ORF Insert Sequence:

ATGGCTTCTT ACTGTGTCAC CGATAGTCCC GAAACTGTGG ACTACAAAAC GAAGTGCTGC 
TGCCGAACGA CGGACAATGT CTACTATGCC GTTCCAAAAG AGTACTTTCG CCTCAATCAG 
AGCCTGGCGC GGCGAAAGCT TCTATTAGCG GAGCCGTTCC CTGTTCCAGT GGACTGCAAA 
ACTCTGGACC ACTTGCTGGT GCTCCTTGAG AAGGCCACAA TTCTCTCCGC TCAGGTTGTG 
GAGGGTGAGA CGAAGGGTAG CAATAACGAG CGTCCGGAGT GGATGCGTGA CTTGAACAAG 
CGTCAGCAGA AGTTTGTTTG CGGGTGCCTG GGCATCACCT CATGGGATGG CAAGGACATT 
CCGTTCTACG TGGAAACGAT GCCCAAGATC AACGACGTCG TTTGGGTGAA AATCACCCAG 
GTGAATGACA CCTCGGCGGT AGTGCAGCTG CTCGAGTACG GCAAGCGCGA AGGCATTATT 
CCGTACACGG AGGTGACTAG GCGCCGTGTG CGCTCGATGG GAAAGCTGAT CAAGGTAGGC 
CGTACCGAGC CGGCGCAGGT GATTCGTATC GACAAGGACA AGGGTTATAT TGACTTGTCA 
AAGAAGCTGG TAACGCCCAA CGAAGCAAAG GCGTGCGAAG CGCACTTCCG CCAGGGGAAC 
GAAGTTCGCT CTATTGTTTG CCATGTCGCT GAGCTGTGCG ATATCCCCGC TATGGATGCA 
ATGGAAATGA TCGCCTATCC GCTTTACCAG CGGGAACCGG GCAAGCACGC GTGGACGTGG 
CTTTACGAAC TGAACCAGAC GGAAGACGTG GAGCGCATTC TGGGTCCGCT GAAGCTTGAC 
AAAGCCATAT CGGACTGCCT GATGTCGACG CTGAAAAACG CGATGCGGCT GAAGGTGTTG 
ACGCTGTTTG CGGAAGTCGA GATCACGTGC TTTGCGTGTG ATGGTGTGGA GGCGATCCGC 
GACGTCCTCA TTCTTGGCCG CAGCTACGGA GAAGGCAGCG ATCCCCAGAT CCACCTGTCC 
GTCAATATCA TCGGACCCCC CAAGTATGGC ATCCGAGCGC GCACGGATAT GAAGGAAGAG 
GGTATTCAGC GAATGAAAGA GGCAATAGAG GCGATGACGG CGGAAATCAA GAAGCGCGGT 
GGCCAGCTGA AGGTGGTGAC GCCTCCGCAG CCTCACGGCG ACGCAGACGA AGGCAAGAAG 
GGTTTGGATG GAGAGGACGA TGATGATGAG GATGCTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_03_0960 cDNA ORF clone, Leishmania infantum JPCM5

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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