IscW_ISCW005295 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	IscW_ISCW005295
Entrez Gene ID	8052466
Full Name	sumo ligase, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029988881.1	XP_029844741.1	E3 SUMO-protein ligase PIAS2 isoform X2
XM_029988882.1	XP_029844742.1	E3 SUMO-protein ligase PIAS2 isoform X3
XM_029988883.1	XP_029844743.1	E3 SUMO-protein ligase PIAS2 isoform X4
XM_029988880.1	XP_029844740.1	E3 SUMO-protein ligase PIAS2 isoform X1
XM_002434620.1	XP_002434665.1	sumo ligase, putative

Pathways from BioSystems
Gene Ontology

The following IscW_ISCW005295 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IscW_ISCW005295 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa30679	XM_029988880.1 Latest version!	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OIa30680	XM_029988881.1 Latest version!	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OIa30681	XM_029988882.1 Latest version!	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OIa30682	XM_029988883.1 Latest version!	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IscW_ISCW005295 ( XM_029988880.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029844740.1 Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa30679
Clone ID Related Accession (Same CDS sequence)	XM_029988880.1
Accession Version	XM_029988880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	E3 SUMO-protein ligase PIAS2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAGTGAG 
CAGCCGCCAC ACCTTGCTCC CGGCCGGTGC CCCACCCCCC CACGCGAAGG GAGGCGCGAC 
TTTGACCTGT ACTCGCTGCT GACCAACGAC TCCGAACGAC CCATGCCCTC GTATTCCACA 
GGACTTGCTC GGAAGGCCAG CTCCACTCCA GACATAATCT CCCTGGACTG CGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029844740.1
CDS	73..2289
Translation	MSDIEEYRQMVLNFRVSELQVLLGFAGRNKSGKKQELQARALELLRTHNTPILMKIRELHKRRFPSTTVAHPPHPSSHGASGGGAEVSDPRLCGGQSLKGPAYASLTRAGALLGGAHHGGVHGGDYPSKLHPPPMLPQATGGAQAPPAPSYPVHPDVRFKQLPFFDVLAELHRPASLMPNSNTRFQENSFVFHFTPQQVHDIATSRDYSPGAKPEFSVQVQLRLCLLETSCEQDDNFPPSMCVKVNSKVCPLPNPIPTNKPGVEPKRPSRPINIVSMCRLSPTVSNHVSVTWLSEYGRAYAIGVYLVRKLTASTLLQRLKATGMRNPDHTRAMIKEKLQHDPDSEIATTSLRGSLICPLGKMRMGIPCRALTCLHLQCFDASLYLQMNEKKPTWICPVCDRPATFTSLVIDGLFMEITMKAPGDCKEVQFHEDGSWSPLVAKKETHLINSPTSASTSRATTSTATRGAESQPSGRSSKRPRVEVIDLTLDSSDDEGDPGSPAPGVGPPPPLVPSSSAGLSLSSAQGGPSTPLGGAPSPSAPLISLPSPSSSSSSSSSSSSSSSAASFSSLLSPPLGGATPTPSSSSAGGAGLQYQNPTLGPSTSGPCSRGLPMPGSGLAQGSGLHESSGSGLAQGSLPSSSLYPGIGVMDASTPPYYPPVSSPFPEYFTSSHPMYHYTSEQPPHLAPGRCPTPPREGRRDFDLYSLLTNDSERPMPSYSTGLARKASSTPDIISLDCD

Target ORF information:

RefSeq Version	XM_029988880.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029988880.1

ORF Insert Sequence:

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAGTGAG 
CAGCCGCCAC ACCTTGCTCC CGGCCGGTGC CCCACCCCCC CACGCGAAGG GAGGCGCGAC 
TTTGACCTGT ACTCGCTGCT GACCAACGAC TCCGAACGAC CCATGCCCTC GTATTCCACA 
GGACTTGCTC GGAAGGCCAG CTCCACTCCA GACATAATCT CCCTGGACTG CGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW005295 ( XM_029988881.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029844741.1 Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIa30680
Clone ID Related Accession (Same CDS sequence)	XM_029988881.1
Accession Version	XM_029988881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	E3 SUMO-protein ligase PIAS2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAGTGAG 
CAGCCGCCAC ACCTTGCTCC CGGCCGGTGC CCCACCCCCC CACGCGAAGG GAGGCGCGAC 
TTTGACCTGT ACTCGCTGCT GACCAACGAC TCCGAACGAC CCGACTTGCT CGGAAGGCCA 
GCTCCACTCC AGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029844741.1
CDS	73..2250
Translation	MSDIEEYRQMVLNFRVSELQVLLGFAGRNKSGKKQELQARALELLRTHNTPILMKIRELHKRRFPSTTVAHPPHPSSHGASGGGAEVSDPRLCGGQSLKGPAYASLTRAGALLGGAHHGGVHGGDYPSKLHPPPMLPQATGGAQAPPAPSYPVHPDVRFKQLPFFDVLAELHRPASLMPNSNTRFQENSFVFHFTPQQVHDIATSRDYSPGAKPEFSVQVQLRLCLLETSCEQDDNFPPSMCVKVNSKVCPLPNPIPTNKPGVEPKRPSRPINIVSMCRLSPTVSNHVSVTWLSEYGRAYAIGVYLVRKLTASTLLQRLKATGMRNPDHTRAMIKEKLQHDPDSEIATTSLRGSLICPLGKMRMGIPCRALTCLHLQCFDASLYLQMNEKKPTWICPVCDRPATFTSLVIDGLFMEITMKAPGDCKEVQFHEDGSWSPLVAKKETHLINSPTSASTSRATTSTATRGAESQPSGRSSKRPRVEVIDLTLDSSDDEGDPGSPAPGVGPPPPLVPSSSAGLSLSSAQGGPSTPLGGAPSPSAPLISLPSPSSSSSSSSSSSSSSSAASFSSLLSPPLGGATPTPSSSSAGGAGLQYQNPTLGPSTSGPCSRGLPMPGSGLAQGSGLHESSGSGLAQGSLPSSSLYPGIGVMDASTPPYYPPVSSPFPEYFTSSHPMYHYTSEQPPHLAPGRCPTPPREGRRDFDLYSLLTNDSERPDLLGRPAPLQT

Target ORF information:

RefSeq Version	XM_029988881.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029988881.1

ORF Insert Sequence:

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAGTGAG 
CAGCCGCCAC ACCTTGCTCC CGGCCGGTGC CCCACCCCCC CACGCGAAGG GAGGCGCGAC 
TTTGACCTGT ACTCGCTGCT GACCAACGAC TCCGAACGAC CCGACTTGCT CGGAAGGCCA 
GCTCCACTCC AGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW005295 ( XM_029988882.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029844742.1 Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OIa30681
Clone ID Related Accession (Same CDS sequence)	XM_029988882.1
Accession Version	XM_029988882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	E3 SUMO-protein ligase PIAS2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAACTTT 
GACCTGTACT CGCTGCTGAC CAACGACTCC GAACGACCCA TGCCCTCGTA TTCCACAGGA 
CTTGCTCGGA AGGCCAGCTC CACTCCAGAC ATAATCTCCC TGGACTGCGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029844742.1
CDS	73..2226
Translation	MSDIEEYRQMVLNFRVSELQVLLGFAGRNKSGKKQELQARALELLRTHNTPILMKIRELHKRRFPSTTVAHPPHPSSHGASGGGAEVSDPRLCGGQSLKGPAYASLTRAGALLGGAHHGGVHGGDYPSKLHPPPMLPQATGGAQAPPAPSYPVHPDVRFKQLPFFDVLAELHRPASLMPNSNTRFQENSFVFHFTPQQVHDIATSRDYSPGAKPEFSVQVQLRLCLLETSCEQDDNFPPSMCVKVNSKVCPLPNPIPTNKPGVEPKRPSRPINIVSMCRLSPTVSNHVSVTWLSEYGRAYAIGVYLVRKLTASTLLQRLKATGMRNPDHTRAMIKEKLQHDPDSEIATTSLRGSLICPLGKMRMGIPCRALTCLHLQCFDASLYLQMNEKKPTWICPVCDRPATFTSLVIDGLFMEITMKAPGDCKEVQFHEDGSWSPLVAKKETHLINSPTSASTSRATTSTATRGAESQPSGRSSKRPRVEVIDLTLDSSDDEGDPGSPAPGVGPPPPLVPSSSAGLSLSSAQGGPSTPLGGAPSPSAPLISLPSPSSSSSSSSSSSSSSSAASFSSLLSPPLGGATPTPSSSSAGGAGLQYQNPTLGPSTSGPCSRGLPMPGSGLAQGSGLHESSGSGLAQGSLPSSSLYPGIGVMDASTPPYYPPVSSPFPEYFTSSHPMYHYTNFDLYSLLTNDSERPMPSYSTGLARKASSTPDIISLDCD

Target ORF information:

RefSeq Version	XM_029988882.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029988882.1

ORF Insert Sequence:

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAACTTT 
GACCTGTACT CGCTGCTGAC CAACGACTCC GAACGACCCA TGCCCTCGTA TTCCACAGGA 
CTTGCTCGGA AGGCCAGCTC CACTCCAGAC ATAATCTCCC TGGACTGCGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW005295 ( XM_029988883.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029844743.1 Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X4, mRNA.

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CloneID	OIa30682
Clone ID Related Accession (Same CDS sequence)	XM_029988883.1
Accession Version	XM_029988883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	E3 SUMO-protein ligase PIAS2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAACTTT 
GACCTGTACT CGCTGCTGAC CAACGACTCC GAACGACCCG ACTTGCTCGG AAGGCCAGCT 
CCACTCCAGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029844743.1
CDS	73..2187
Translation	MSDIEEYRQMVLNFRVSELQVLLGFAGRNKSGKKQELQARALELLRTHNTPILMKIRELHKRRFPSTTVAHPPHPSSHGASGGGAEVSDPRLCGGQSLKGPAYASLTRAGALLGGAHHGGVHGGDYPSKLHPPPMLPQATGGAQAPPAPSYPVHPDVRFKQLPFFDVLAELHRPASLMPNSNTRFQENSFVFHFTPQQVHDIATSRDYSPGAKPEFSVQVQLRLCLLETSCEQDDNFPPSMCVKVNSKVCPLPNPIPTNKPGVEPKRPSRPINIVSMCRLSPTVSNHVSVTWLSEYGRAYAIGVYLVRKLTASTLLQRLKATGMRNPDHTRAMIKEKLQHDPDSEIATTSLRGSLICPLGKMRMGIPCRALTCLHLQCFDASLYLQMNEKKPTWICPVCDRPATFTSLVIDGLFMEITMKAPGDCKEVQFHEDGSWSPLVAKKETHLINSPTSASTSRATTSTATRGAESQPSGRSSKRPRVEVIDLTLDSSDDEGDPGSPAPGVGPPPPLVPSSSAGLSLSSAQGGPSTPLGGAPSPSAPLISLPSPSSSSSSSSSSSSSSSAASFSSLLSPPLGGATPTPSSSSAGGAGLQYQNPTLGPSTSGPCSRGLPMPGSGLAQGSGLHESSGSGLAQGSLPSSSLYPGIGVMDASTPPYYPPVSSPFPEYFTSSHPMYHYTNFDLYSLLTNDSERPDLLGRPAPLQT

Target ORF information:

RefSeq Version	XM_029988883.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis E3 SUMO-protein ligase PIAS2 (LOC8052466), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029988883.1

ORF Insert Sequence:

ATGTCGGATA TTGAAGAATA CCGACAAATG GTCCTGAATT TCCGGGTGTC GGAGCTCCAG 
GTGCTCCTCG GTTTTGCGGG CCGCAACAAG TCTGGGAAGA AGCAGGAACT GCAGGCCAGG 
GCACTGGAGC TGCTTCGCAC GCACAACACC CCCATCCTCA TGAAGATCAG GGAGCTGCAC 
AAGCGCCGCT TCCCATCCAC AACGGTGGCC CACCCGCCTC ACCCGTCCTC GCACGGGGCC 
TCGGGCGGCG GTGCCGAGGT GTCGGACCCC CGGCTGTGCG GGGGACAGAG CCTGAAGGGG 
CCGGCGTACG CCTCTCTCAC CCGGGCCGGG GCCCTGCTGG GGGGGGCCCA CCATGGGGGG 
GTGCACGGGG GCGACTACCC GTCGAAGCTG CACCCCCCGC CCATGCTGCC CCAGGCGACG 
GGAGGGGCCC AGGCCCCCCC TGCACCCTCG TACCCCGTCC ACCCGGACGT GCGCTTCAAG 
CAGCTGCCCT TCTTCGATGT GCTGGCGGAG CTGCACCGGC CGGCAAGCCT CATGCCCAAC 
AGTAACACGA GGTTCCAGGA GAACAGTTTC GTCTTCCACT TCACGCCGCA GCAGGTGCAC 
GACATCGCCA CGTCACGGGA CTACAGCCCC GGGGCGAAGC CGGAGTTCTC GGTGCAGGTG 
CAGCTACGGC TGTGCCTGCT GGAGACGAGC TGCGAGCAGG ACGACAACTT TCCGCCCAGC 
ATGTGCGTGA AGGTCAACTC GAAGGTGTGC CCCCTGCCCA ACCCCATCCC CACCAACAAG 
CCGGGGGTGG AGCCCAAGCG GCCCAGCCGG CCGATCAACA TCGTCTCCAT GTGTCGCCTC 
TCGCCCACAG TCTCCAACCA CGTGTCGGTC ACCTGGCTCT CGGAGTACGG CAGGGCGTAC 
GCCATCGGCG TGTACCTGGT GCGCAAGTTG ACGGCGTCCA CACTGCTTCA GCGGCTCAAG 
GCCACGGGCA TGCGGAACCC CGATCACACG AGGGCGATGA TCAAAGAGAA GCTGCAACAC 
GACCCGGATT CAGAGATAGC AACCACCAGC CTACGAGGTT CTCTCATCTG CCCCCTGGGC 
AAGATGCGGA TGGGAATCCC GTGCCGCGCC CTGACGTGCC TGCACCTCCA GTGCTTCGAT 
GCCTCCCTGT ACCTCCAGAT GAACGAAAAG AAACCCACCT GGATCTGCCC AGTGTGCGAC 
CGCCCGGCAA CCTTCACCTC GCTCGTCATC GACGGGCTGT TCATGGAGAT CACGATGAAG 
GCGCCCGGGG ACTGCAAAGA GGTTCAGTTC CACGAAGACG GCTCCTGGTC CCCCCTTGTG 
GCAAAGAAGG AAACCCACCT GATCAACAGC CCCACCTCGG CATCCACTTC GAGGGCGACC 
ACCTCCACAG CCACACGGGG AGCAGAAAGC CAGCCGAGCG GGCGCAGCAG CAAGCGTCCT 
CGGGTGGAGG TCATCGACCT GACGCTAGAC TCCAGCGACG ACGAAGGGGA CCCCGGCAGC 
CCGGCCCCCG GGGTGGGGCC GCCTCCCCCG CTGGTGCCTT CCTCCTCGGC CGGCCTGTCC 
CTGTCTTCTG CGCAGGGCGG TCCGAGCACC CCCCTGGGCG GCGCCCCGTC CCCCTCGGCC 
CCCCTCATCT CCCTGCCCTC CCCGTCGTCG TCTTCCTCTT CCTCCTCGTC GTCCTCCTCT 
TCGTCCTCTG CAGCCTCCTT CTCCAGCCTG CTGAGCCCTC CCCTGGGGGG CGCCACCCCG 
ACTCCGTCGT CCTCCTCGGC GGGGGGCGCC GGCCTTCAGT ATCAGAACCC CACGCTGGGA 
CCCTCCACCT CTGGGCCCTG CTCCAGGGGA CTGCCCATGC CAGGGAGCGG GCTGGCACAG 
GGCAGCGGGC TGCACGAGTC GTCCGGCTCC GGCCTGGCCC AGGGGAGCCT GCCCAGCAGC 
TCCCTGTACC CGGGCATTGG CGTGATGGAT GCCAGCACGC CGCCCTACTA CCCACCGGTC 
TCCAGCCCCT TCCCGGAGTA CTTCACCTCG TCGCACCCCA TGTACCACTA TACCAACTTT 
GACCTGTACT CGCTGCTGAC CAACGACTCC GAACGACCCG ACTTGCTCGG AAGGCCAGCT 
CCACTCCAGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IscW_ISCW005295 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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