LOC8036613 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8036613
Entrez Gene ID	8036613
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002402979.1	XP_002403023.1	hypothetical protein
XM_029973201.1	XP_029829061.1	protein madd-4 isoform X1
XM_029973202.1	XP_029829062.1	protein madd-4 isoform X2
XM_029973203.1	XP_029829063.1	protein madd-4 isoform X3
XM_029973204.1	XP_029829064.1	protein madd-4 isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC8036613 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8036613 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa07621	XM_002402979.1 Latest version!	Ixodes scapularis hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OIa42623	XM_029973201.1 Latest version!	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OIa42624	XM_029973202.1 Latest version!	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OIa42625	XM_029973203.1 Latest version!	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OIa42626	XM_029973204.1 Latest version!	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8036613 ( XM_002402979.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002403023.1 Ixodes scapularis hypothetical protein, mRNA.

Price & Availability↑

CloneID	OIa07621
Clone ID Related Accession (Same CDS sequence)	XM_002402979.1
Accession Version	XM_002402979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002760836). COMPLETENESS: incomplete on both ends.

ATGTCTTTAT TAAAAGGAGT TTGTCATCCC TTATCTACAG GTATCAAGAA GAGGCCGTGC 
CCGGAGCACG TGGACTTCAG GGCCACGCAG TGCTCGGAGT TCGACCAGGT GCCCTACCGG 
GGACGCCTGT ACGAGTGGAC GCCTGTGCAC GACGCCAGCG ACCCGTGCAG CCTTACCTGC 
CAGGCCAGGG AGTTCAAGTT CATGGCCAAG CTGGCGCCCC GGGCCCAGGA CGGCACGCGC 
TGTCGCCAGG GGTCCCTCGA CATGTGCGTC CAGGGAAAGT GCCTGCCCGT CGGCTGTGAC 
TTGGAGCTCG GCTCCTACCT CGAGGTGGAC GAGTGCGGCA TTTGTGGAGG CAACGGGACG 
TCCTGCCGAG CACCTGCCTT CACCTGGTCC GAGACGCCCT TCTCGGAGTG CTCGGTGACG 
TGTGGTGGGG GCTTCCAGGA GAGTCATCCT GTCTGCACGT CTTCAGACAC TGGTTCTGAA 
GTAGACGACC GGTTGTGCAA TGTCGACGAG AGGCCCGACT CTCGGGTCCG AAAGTGCAAT 
GGCAATCCCT GTCCCCCCGG GTGGCAGATG GGCGCTTGGG GCGACTGCAG CGCCTCGTGC 
GGCGGCGGCC AGCAGGTGCG CAACGTATCG TGCGTGCGCC CCACGGTGCC CCCCGTTCCA 
GTGGCAGAGC ACTTCTGCCC CGGCCAGCGA CCCGCAACGT CCCAAAGCTG CAACCTCCAC 
GACTGTTCCC GCTGGACGGA GGGCCAGTGG TCACAGACAC GGGCGGTGGA GTGCCGTGAC 
CTCCGCGGCC GCAAGAGCCA CCTGTGCGAC CCCAAGCGGC AAGCCTCGGT CCAAGCAACA 
GTGCTCCGCG GGAAACCCCT GCTTCTCAAA GGTAAAGAGT ATGGATCGCA AGAGGACGCA 
CCACCCCAGA GTTTCCCGCC GTTCAACATC TTCTCCAAAC CACAAAACCA GTCACCGGGG 
TCGGAACCGA GCTTCATCGT CGGAGACTGG GGATCCTGTA GCGTCACGTG CGGCGATGGA 
GTCCAGAAGC GCGCCGTGGA GTGCAAGATC TTCCTGGAGT TCTCCAGAAC GATGGCGAAG 
CTTCCGGACA AGGAGTGCAC AGGAATCCGA CCGGCCGAAC TGCAGCGTTG CACGATGAGA 
CCGTGCGCCA TGGACCAGAA CCGCGAGGCT CTGACGGAGG AGGAACCAAC GGACCAAGGA 
GTCGTCACAA CGTATTCGTG GAGGAGCGCC GGTTACACTC CGTGCACGGC GTCTTGCCTC 
GGAGGTATTC GAGAGTCACT GGTCCACTGC GTTCGTAACG AAGACGGAGT TCCTGTCAGC 
TACATCCAGT GCGACATCAG AGACAAGCCC GAAACAATCA CCCAGACCTG CAATAACCGG 
CCCTGTCCCC CAAGGTGGAA CACGTCCGAC TTCACGGCGT GCAGCAAGCC GTGTGGCGGT 
GGTTCCCAGA CGCGCACCGT GCAGTGCGTG CACGAGGTGA CCCGGGGTGT CGGAAACACC 
CTGGTGGTAC CAGACACCCA CTGCCCGCTG CCACGCCCGG CCGAACACCG CATGTGCAAC 
ATGGTTGACT GCGTCGCGCA CTGGACTGCC GACAGTCGTG CCAAGTGGCT CAAGGACGGC 
CTCCCCGTCA AGACCTCCTC GAGACGGGTG GTCGTGTCGA GAAAGGGAGG CCTGCGGATC 
AGGAAGGCAG ACTTCCCAGA CGCCGGCACC TACGTCTGCT CCGCGGGAAC CTCCAAGAGC 
GAGCTTCTGG ACCGAGCAAC CTCCTCCGAC GTGGACCGGA CCGCCTTCAA GGTGGACCTC 
GCTTCGGACC AAGAACGCAA CGATCAAGGA CTCCGAGAGG TGAAGGGGAC CCTCGAAGAT 
CCGACAGTCA ACTCGGGTGC ATCTAGTCGG CCCGGTGGAA ACATTCCGCG CATCCAGACA 
CTGCTGTCGG ACTTTCACCG GCGGCTCGGC GGCAGTGGTG CGGTCAGGGG CACTGTCGAA 
CTCCCACCCG ACGATGAGGA CCAGCTGGGC GTGCCCAACA GCTACGTTCT CGGGCGTGGG 
AACGCAGACA ACCTCCAGTT CGAGTGGTCC ACGACTCCCT GGTCCGCCTG CTCACAAACG 
TGCGGAGGCA GCGGACTCCA GGTGCGTTCC ACACAGTGCA TGGTGCGGCT CCACAACGTC 
TCTCGAACCG TGGACAGCAC GCTGTGTGCC GGTCTCGAAG CCCCCCAGAT CCTGCAGCGC 
TGCGGTTCGG CAGGGTGCCC ACTCTGGGTC ACCGGAAGCT GGTCCCAGTG TTCCGACGGC 
CGCTGCTTCA CGCTGAGCAC GTCGGTGCAA GAGCGCCTGG TGGAGTGCCG GCTTCCCGAC 
GGCACCCCAG TCGACGAGAA GTTGTGCGAC GCCTCGACGC GCCCGCAGGA CCAGCAGGAG 
TGCCTCAGCG AGCGCTGCCG CGGCGTCTGG AAGGTTGGAG ACTGGTCAGA GTGCACGGCG 
ACGTGCGGTG AGAGCGGTTT CCGGACGCGG ATCCTGCGCT GCGTTTGGTA CGGCACCGAC 
AAGCCGGCCG GAGACTCCTG CCGCAATCAG GACCGGCCCG AAGTGCTCCA AGGCTGCGAT 
GCTCCCCCTT GCAACGCCAC CAGTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002403023.1
CDS	1..2610
Translation	MSLLKGVCHPLSTGIKKRPCPEHVDFRATQCSEFDQVPYRGRLYEWTPVHDASDPCSLTCQAREFKFMAKLAPRAQDGTRCRQGSLDMCVQGKCLPVGCDLELGSYLEVDECGICGGNGTSCRAPAFTWSETPFSECSVTCGGGFQESHPVCTSSDTGSEVDDRLCNVDERPDSRVRKCNGNPCPPGWQMGAWGDCSASCGGGQQVRNVSCVRPTVPPVPVAEHFCPGQRPATSQSCNLHDCSRWTEGQWSQTRAVECRDLRGRKSHLCDPKRQASVQATVLRGKPLLLKGKEYGSQEDAPPQSFPPFNIFSKPQNQSPGSEPSFIVGDWGSCSVTCGDGVQKRAVECKIFLEFSRTMAKLPDKECTGIRPAELQRCTMRPCAMDQNREALTEEEPTDQGVVTTYSWRSAGYTPCTASCLGGIRESLVHCVRNEDGVPVSYIQCDIRDKPETITQTCNNRPCPPRWNTSDFTACSKPCGGGSQTRTVQCVHEVTRGVGNTLVVPDTHCPLPRPAEHRMCNMVDCVAHWTADSRAKWLKDGLPVKTSSRRVVVSRKGGLRIRKADFPDAGTYVCSAGTSKSELLDRATSSDVDRTAFKVDLASDQERNDQGLREVKGTLEDPTVNSGASSRPGGNIPRIQTLLSDFHRRLGGSGAVRGTVELPPDDEDQLGVPNSYVLGRGNADNLQFEWSTTPWSACSQTCGGSGLQVRSTQCMVRLHNVSRTVDSTLCAGLEAPQILQRCGSAGCPLWVTGSWSQCSDGRCFTLSTSVQERLVECRLPDGTPVDEKLCDASTRPQDQQECLSERCRGVWKVGDWSECTATCGESGFRTRILRCVWYGTDKPAGDSCRNQDRPEVLQGCDAPPCNATSE

Target ORF information:

RefSeq Version	XM_002402979.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002402979.1

ORF Insert Sequence:

ATGTCTTTAT TAAAAGGAGT TTGTCATCCC TTATCTACAG GTATCAAGAA GAGGCCGTGC 
CCGGAGCACG TGGACTTCAG GGCCACGCAG TGCTCGGAGT TCGACCAGGT GCCCTACCGG 
GGACGCCTGT ACGAGTGGAC GCCTGTGCAC GACGCCAGCG ACCCGTGCAG CCTTACCTGC 
CAGGCCAGGG AGTTCAAGTT CATGGCCAAG CTGGCGCCCC GGGCCCAGGA CGGCACGCGC 
TGTCGCCAGG GGTCCCTCGA CATGTGCGTC CAGGGAAAGT GCCTGCCCGT CGGCTGTGAC 
TTGGAGCTCG GCTCCTACCT CGAGGTGGAC GAGTGCGGCA TTTGTGGAGG CAACGGGACG 
TCCTGCCGAG CACCTGCCTT CACCTGGTCC GAGACGCCCT TCTCGGAGTG CTCGGTGACG 
TGTGGTGGGG GCTTCCAGGA GAGTCATCCT GTCTGCACGT CTTCAGACAC TGGTTCTGAA 
GTAGACGACC GGTTGTGCAA TGTCGACGAG AGGCCCGACT CTCGGGTCCG AAAGTGCAAT 
GGCAATCCCT GTCCCCCCGG GTGGCAGATG GGCGCTTGGG GCGACTGCAG CGCCTCGTGC 
GGCGGCGGCC AGCAGGTGCG CAACGTATCG TGCGTGCGCC CCACGGTGCC CCCCGTTCCA 
GTGGCAGAGC ACTTCTGCCC CGGCCAGCGA CCCGCAACGT CCCAAAGCTG CAACCTCCAC 
GACTGTTCCC GCTGGACGGA GGGCCAGTGG TCACAGACAC GGGCGGTGGA GTGCCGTGAC 
CTCCGCGGCC GCAAGAGCCA CCTGTGCGAC CCCAAGCGGC AAGCCTCGGT CCAAGCAACA 
GTGCTCCGCG GGAAACCCCT GCTTCTCAAA GGTAAAGAGT ATGGATCGCA AGAGGACGCA 
CCACCCCAGA GTTTCCCGCC GTTCAACATC TTCTCCAAAC CACAAAACCA GTCACCGGGG 
TCGGAACCGA GCTTCATCGT CGGAGACTGG GGATCCTGTA GCGTCACGTG CGGCGATGGA 
GTCCAGAAGC GCGCCGTGGA GTGCAAGATC TTCCTGGAGT TCTCCAGAAC GATGGCGAAG 
CTTCCGGACA AGGAGTGCAC AGGAATCCGA CCGGCCGAAC TGCAGCGTTG CACGATGAGA 
CCGTGCGCCA TGGACCAGAA CCGCGAGGCT CTGACGGAGG AGGAACCAAC GGACCAAGGA 
GTCGTCACAA CGTATTCGTG GAGGAGCGCC GGTTACACTC CGTGCACGGC GTCTTGCCTC 
GGAGGTATTC GAGAGTCACT GGTCCACTGC GTTCGTAACG AAGACGGAGT TCCTGTCAGC 
TACATCCAGT GCGACATCAG AGACAAGCCC GAAACAATCA CCCAGACCTG CAATAACCGG 
CCCTGTCCCC CAAGGTGGAA CACGTCCGAC TTCACGGCGT GCAGCAAGCC GTGTGGCGGT 
GGTTCCCAGA CGCGCACCGT GCAGTGCGTG CACGAGGTGA CCCGGGGTGT CGGAAACACC 
CTGGTGGTAC CAGACACCCA CTGCCCGCTG CCACGCCCGG CCGAACACCG CATGTGCAAC 
ATGGTTGACT GCGTCGCGCA CTGGACTGCC GACAGTCGTG CCAAGTGGCT CAAGGACGGC 
CTCCCCGTCA AGACCTCCTC GAGACGGGTG GTCGTGTCGA GAAAGGGAGG CCTGCGGATC 
AGGAAGGCAG ACTTCCCAGA CGCCGGCACC TACGTCTGCT CCGCGGGAAC CTCCAAGAGC 
GAGCTTCTGG ACCGAGCAAC CTCCTCCGAC GTGGACCGGA CCGCCTTCAA GGTGGACCTC 
GCTTCGGACC AAGAACGCAA CGATCAAGGA CTCCGAGAGG TGAAGGGGAC CCTCGAAGAT 
CCGACAGTCA ACTCGGGTGC ATCTAGTCGG CCCGGTGGAA ACATTCCGCG CATCCAGACA 
CTGCTGTCGG ACTTTCACCG GCGGCTCGGC GGCAGTGGTG CGGTCAGGGG CACTGTCGAA 
CTCCCACCCG ACGATGAGGA CCAGCTGGGC GTGCCCAACA GCTACGTTCT CGGGCGTGGG 
AACGCAGACA ACCTCCAGTT CGAGTGGTCC ACGACTCCCT GGTCCGCCTG CTCACAAACG 
TGCGGAGGCA GCGGACTCCA GGTGCGTTCC ACACAGTGCA TGGTGCGGCT CCACAACGTC 
TCTCGAACCG TGGACAGCAC GCTGTGTGCC GGTCTCGAAG CCCCCCAGAT CCTGCAGCGC 
TGCGGTTCGG CAGGGTGCCC ACTCTGGGTC ACCGGAAGCT GGTCCCAGTG TTCCGACGGC 
CGCTGCTTCA CGCTGAGCAC GTCGGTGCAA GAGCGCCTGG TGGAGTGCCG GCTTCCCGAC 
GGCACCCCAG TCGACGAGAA GTTGTGCGAC GCCTCGACGC GCCCGCAGGA CCAGCAGGAG 
TGCCTCAGCG AGCGCTGCCG CGGCGTCTGG AAGGTTGGAG ACTGGTCAGA GTGCACGGCG 
ACGTGCGGTG AGAGCGGTTT CCGGACGCGG ATCCTGCGCT GCGTTTGGTA CGGCACCGAC 
AAGCCGGCCG GAGACTCCTG CCGCAATCAG GACCGGCCCG AAGTGCTCCA AGGCTGCGAT 
GCTCCCCCTT GCAACGCCAC CAGTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8036613 ( XM_029973201.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029829061.1 Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa42623
Clone ID Related Accession (Same CDS sequence)	XM_029973201.1
Accession Version	XM_029973201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	protein madd-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021924022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGTG CTCCGCGACG TGTGGCGTGG GCACGCAGAC ACGGGCGGTG 
GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC CACCTGTGCG ACCCCAAGCG CAAGCCTCGG 
TCCAAGCAAC AGTGCTCCGC GGGAAACCCC TGCTTCTCCA AAGGTAAAGA GTATGGATCG 
CAAGAGGACG CACCACCCCA GAGTTTCCCG CCCTTCAACA TCTTCTCCAA ACCACAAAAC 
CAGTCACCGG GGTCGGAACC GAGCTTCATC GTCGGAGACT GGGGATCCTG TAGCGTCACG 
TGCGGCGATG GAGTCCAGAA GCGCGCCGTG GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA 
ACGATGGCGA AGCTTCCGGA CAAGGAGTGC ACAGGAATCC GACCGGCCGA ACTGCAACGT 
TGCACGATGA GACCGTGCGC CATGGACCAG AACCGCGAGG CTCTGACGGA GGAGGAACCA 
ACGGACCAAG GAGTCGTCAC AACGTATTCG TGGAGGAGCG CCGGTTACAC TCCGTGCACG 
GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA CTGGTCCACT GCGTTCGTAA TGAAGACGGA 
GTTCCTGTCA GCTACATCCA GTGTGACATC AGAGACAAGC CTGAAACAAT CACCCAGACC 
TGCAATAACC GGCCCTGTCC CCCAAGGTGG AACACGTCCG ACTTCACGGC GTGCAGCAAG 
CCGTGTGGCG GTGGTTCCCA GACGCGCACC GTGCAGTGCG TGCACGAGGT GACCCGGGGT 
GTCGGAAACA CCCTGGTGGT ACCAGACACC CACTGCCCGC TGCCACGCCC GGCCGAACAC 
CGCATGTGCA ACATGGTTGA CTGCGTCGCG CACTGGACTG CCGACAGGTG GGCCAAGTGC 
TCCAAGAGCT GTGACGGCGG GGTGAAGACG CGCAAGATCC ACTGTCAACG GGAACTGGCC 
TACGGAACGT TGGCGGACCT GCCGCCGTCA AAGTGTGCCT CGAAGAGAAA GCCTCGTACT 
GTGAAGCCCT GCAACACCAA GCCCTGCCGC AGGGGAAACC CCAGTTCAGT GCAGGGTGGA 
CCTAAGCGAA AGGTGTCCCT GATAGCCGAG GGAAAGGCTT CTGTCTTCCG AGGAACGACG 
CTCAAAATAC GCTGCCCAGG TGTCGTCGCC AACACTTCGT CTGACACAAG CAGTCGTGCC 
AAGTGGCTCA AGGACGGCCT CCCCGTCAAG ACCTCCTCGA GACGGGTGGT CGTGTCGAGA 
AAGGGAGGCC TGCGGATCAG GAAGGCGGAC TTCCCAGACG CCGGCACCTA CGTCTGCTCC 
ACGGGAACCT CCAAGAGCGA GCTCGTGCTT TCCATCCGAC CGCTCTCCTC GCCACCTCCA 
GACACTTCAC AAGGACTTCC CTTTGACGTG CTCAAGGGAC AGAAAGGTTT GTCAACAGCA 
GACGGTGGTA CTTTTGCTTC GAAAGGTCAC CACCCCAACG GAGTTGGCTT GAAGCCAGCC 
AAAGGAGCCG ATCTGCCGTC GTACATCAAC AGTGTCGAAG ACGTGCAGAC GAACAAGGAA 
CAAACTCTCT CCCAGGACAC AAAGCTGGAC CGAGCAACCT CCTCCGACGT GGACCGGACC 
GCCTTCAAGG TGGACCTCGC TTCGGACCAA GAACGCAACG ATCAAGGACT CCGAGAGGTG 
AAGGGGACCC TCGAAGATCC GACAGTCAAC TCGGGTGCAT CTAGTCGGCC CGGTGGAAAC 
ATTCCGCGCA TCCAGACACT GCTGTCGGAC TTTCACCGGC GGCTCGGCGG CAGTGGTGGT 
GGCAGAGACA GCGCGGTCAG GGGCACTGTC GAACTCCCAC CCGACGATGA GGACCAGCTG 
GGCGTGCCCA ACAGCTACGT TCTCGGGCGT GGGAACGCAG ACAACCTCCA ATTCGAGTGG 
TCCACGACTC CCTGGTCCGC CTGCTCACAA ACGTGCGGAG GCAGCGGACT CCAGGTGCGT 
TCCACACAGT GCATGGTGCG GCTCCACAAC GTCTCTCGGA CCGTGGACAG CACGCTGTGC 
GCTGGTCTCG AAGCCCCCCA GATCCTGCAG CGCTGCGGTT CGGCAGGCTG CCCACTCTGG 
GTCACTGGAA GCTGGTCCCA GTGTTCCGAC GGCCGCTGCT TCACGCTGAG CACGTCGGTG 
CAAGAGCGCC TGGTGGAGTG CCGGCTTCCC GACGGCACCC CAGTCGACGA GAAGTTGTGC 
GACGCCTCGA CGCGCCCGCA GGACCAGCAG GAGTGCCTCA GCGAGCGCTG CCGCGGCGTC 
TGGAAGGTTG GAGACTGGTC AGAGTGCACG GCGACGTGCG GTGAGAGCGG TTTCCGGACG 
CGGATCCTGC GCTGCGTTTG GTACGGCACC GACAAGCCGG CCGGAGACTC CTGCCGCAAT 
CAGGACCGGC CCGAAGTGCT TCAAGGCTGC GATGCTCCCC CTTGCAACGC CACCAGGCAG 
ACATGCGAAG ACCACTCCAT CAAGTGTCAC ATGATCCGCC GCTTCAGCAT GTGCCACATA 
AACAAGTACC ATGACCTGTG CTGTGCCACC TGCGAGCGCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029829061.1
CDS	14..3538
Translation	MLPLLGRHRTLLLGTLAVALSWGTRCLHASSALDGETIADLIEAESDDGWSPWGPWSACSRTCDGGVTLQSRRCSRFGCVGESTRSRICNMQPCPEHVDFRATQCSEFDQVPYRGRLYEWTPVHDASDPCSLTCQAREFKFMAKLAPRAQDGTRCRQGALDMCVQGKCLPVGCDLELGSYLEVDECGICGGNGTSCRAPAFTWSETPFSECSVTCGGGFQESHPVCTSSDTGSEVDDRLCNVDERPDSRVRKCNGNPCPPGWQMGAWGDCSASCGGGQQVRNVSCVRPTVPPVPVAEHFCPGQRPATSQSCNLHDCSRWTEGQWSQCSATCGVGTQTRAVECRDLRGRKSHLCDPKRKPRSKQQCSAGNPCFSKGKEYGSQEDAPPQSFPPFNIFSKPQNQSPGSEPSFIVGDWGSCSVTCGDGVQKRAVECKIFLEFSRTMAKLPDKECTGIRPAELQRCTMRPCAMDQNREALTEEEPTDQGVVTTYSWRSAGYTPCTASCLGGIRESLVHCVRNEDGVPVSYIQCDIRDKPETITQTCNNRPCPPRWNTSDFTACSKPCGGGSQTRTVQCVHEVTRGVGNTLVVPDTHCPLPRPAEHRMCNMVDCVAHWTADRWAKCSKSCDGGVKTRKIHCQRELAYGTLADLPPSKCASKRKPRTVKPCNTKPCRRGNPSSVQGGPKRKVSLIAEGKASVFRGTTLKIRCPGVVANTSSDTSSRAKWLKDGLPVKTSSRRVVVSRKGGLRIRKADFPDAGTYVCSTGTSKSELVLSIRPLSSPPPDTSQGLPFDVLKGQKGLSTADGGTFASKGHHPNGVGLKPAKGADLPSYINSVEDVQTNKEQTLSQDTKLDRATSSDVDRTAFKVDLASDQERNDQGLREVKGTLEDPTVNSGASSRPGGNIPRIQTLLSDFHRRLGGSGGGRDSAVRGTVELPPDDEDQLGVPNSYVLGRGNADNLQFEWSTTPWSACSQTCGGSGLQVRSTQCMVRLHNVSRTVDSTLCAGLEAPQILQRCGSAGCPLWVTGSWSQCSDGRCFTLSTSVQERLVECRLPDGTPVDEKLCDASTRPQDQQECLSERCRGVWKVGDWSECTATCGESGFRTRILRCVWYGTDKPAGDSCRNQDRPEVLQGCDAPPCNATRQTCEDHSIKCHMIRRFSMCHINKYHDLCCATCERS

Target ORF information:

RefSeq Version	XM_029973201.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029973201.1

ORF Insert Sequence:

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGTG CTCCGCGACG TGTGGCGTGG GCACGCAGAC ACGGGCGGTG 
GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC CACCTGTGCG ACCCCAAGCG CAAGCCTCGG 
TCCAAGCAAC AGTGCTCCGC GGGAAACCCC TGCTTCTCCA AAGGTAAAGA GTATGGATCG 
CAAGAGGACG CACCACCCCA GAGTTTCCCG CCCTTCAACA TCTTCTCCAA ACCACAAAAC 
CAGTCACCGG GGTCGGAACC GAGCTTCATC GTCGGAGACT GGGGATCCTG TAGCGTCACG 
TGCGGCGATG GAGTCCAGAA GCGCGCCGTG GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA 
ACGATGGCGA AGCTTCCGGA CAAGGAGTGC ACAGGAATCC GACCGGCCGA ACTGCAACGT 
TGCACGATGA GACCGTGCGC CATGGACCAG AACCGCGAGG CTCTGACGGA GGAGGAACCA 
ACGGACCAAG GAGTCGTCAC AACGTATTCG TGGAGGAGCG CCGGTTACAC TCCGTGCACG 
GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA CTGGTCCACT GCGTTCGTAA TGAAGACGGA 
GTTCCTGTCA GCTACATCCA GTGTGACATC AGAGACAAGC CTGAAACAAT CACCCAGACC 
TGCAATAACC GGCCCTGTCC CCCAAGGTGG AACACGTCCG ACTTCACGGC GTGCAGCAAG 
CCGTGTGGCG GTGGTTCCCA GACGCGCACC GTGCAGTGCG TGCACGAGGT GACCCGGGGT 
GTCGGAAACA CCCTGGTGGT ACCAGACACC CACTGCCCGC TGCCACGCCC GGCCGAACAC 
CGCATGTGCA ACATGGTTGA CTGCGTCGCG CACTGGACTG CCGACAGGTG GGCCAAGTGC 
TCCAAGAGCT GTGACGGCGG GGTGAAGACG CGCAAGATCC ACTGTCAACG GGAACTGGCC 
TACGGAACGT TGGCGGACCT GCCGCCGTCA AAGTGTGCCT CGAAGAGAAA GCCTCGTACT 
GTGAAGCCCT GCAACACCAA GCCCTGCCGC AGGGGAAACC CCAGTTCAGT GCAGGGTGGA 
CCTAAGCGAA AGGTGTCCCT GATAGCCGAG GGAAAGGCTT CTGTCTTCCG AGGAACGACG 
CTCAAAATAC GCTGCCCAGG TGTCGTCGCC AACACTTCGT CTGACACAAG CAGTCGTGCC 
AAGTGGCTCA AGGACGGCCT CCCCGTCAAG ACCTCCTCGA GACGGGTGGT CGTGTCGAGA 
AAGGGAGGCC TGCGGATCAG GAAGGCGGAC TTCCCAGACG CCGGCACCTA CGTCTGCTCC 
ACGGGAACCT CCAAGAGCGA GCTCGTGCTT TCCATCCGAC CGCTCTCCTC GCCACCTCCA 
GACACTTCAC AAGGACTTCC CTTTGACGTG CTCAAGGGAC AGAAAGGTTT GTCAACAGCA 
GACGGTGGTA CTTTTGCTTC GAAAGGTCAC CACCCCAACG GAGTTGGCTT GAAGCCAGCC 
AAAGGAGCCG ATCTGCCGTC GTACATCAAC AGTGTCGAAG ACGTGCAGAC GAACAAGGAA 
CAAACTCTCT CCCAGGACAC AAAGCTGGAC CGAGCAACCT CCTCCGACGT GGACCGGACC 
GCCTTCAAGG TGGACCTCGC TTCGGACCAA GAACGCAACG ATCAAGGACT CCGAGAGGTG 
AAGGGGACCC TCGAAGATCC GACAGTCAAC TCGGGTGCAT CTAGTCGGCC CGGTGGAAAC 
ATTCCGCGCA TCCAGACACT GCTGTCGGAC TTTCACCGGC GGCTCGGCGG CAGTGGTGGT 
GGCAGAGACA GCGCGGTCAG GGGCACTGTC GAACTCCCAC CCGACGATGA GGACCAGCTG 
GGCGTGCCCA ACAGCTACGT TCTCGGGCGT GGGAACGCAG ACAACCTCCA ATTCGAGTGG 
TCCACGACTC CCTGGTCCGC CTGCTCACAA ACGTGCGGAG GCAGCGGACT CCAGGTGCGT 
TCCACACAGT GCATGGTGCG GCTCCACAAC GTCTCTCGGA CCGTGGACAG CACGCTGTGC 
GCTGGTCTCG AAGCCCCCCA GATCCTGCAG CGCTGCGGTT CGGCAGGCTG CCCACTCTGG 
GTCACTGGAA GCTGGTCCCA GTGTTCCGAC GGCCGCTGCT TCACGCTGAG CACGTCGGTG 
CAAGAGCGCC TGGTGGAGTG CCGGCTTCCC GACGGCACCC CAGTCGACGA GAAGTTGTGC 
GACGCCTCGA CGCGCCCGCA GGACCAGCAG GAGTGCCTCA GCGAGCGCTG CCGCGGCGTC 
TGGAAGGTTG GAGACTGGTC AGAGTGCACG GCGACGTGCG GTGAGAGCGG TTTCCGGACG 
CGGATCCTGC GCTGCGTTTG GTACGGCACC GACAAGCCGG CCGGAGACTC CTGCCGCAAT 
CAGGACCGGC CCGAAGTGCT TCAAGGCTGC GATGCTCCCC CTTGCAACGC CACCAGGCAG 
ACATGCGAAG ACCACTCCAT CAAGTGTCAC ATGATCCGCC GCTTCAGCAT GTGCCACATA 
AACAAGTACC ATGACCTGTG CTGTGCCACC TGCGAGCGCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8036613 ( XM_029973202.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029829062.1 Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X2, mRNA.

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CloneID	OIa42624
Clone ID Related Accession (Same CDS sequence)	XM_029973202.1
Accession Version	XM_029973202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	protein madd-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021924022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGTG CTCCGCGACG TGTGGCGTGG GCACGCAGAC ACGGGCGGTG 
GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC CACCTGTGCG ACCCCAAGCG CAAGCCTCGG 
TCCAAGCAAC AGTGCTCCGC GGGAAACCCC TGCTTCTCCA AAGGTAAAGA GTATGGATCG 
CAAGAGGACG CACCACCCCA GAGTTTCCCG CCCTTCAACA TCTTCTCCAA ACCACAAAAC 
CAGTCACCGG GGTCGGAACC GAGCTTCATC GTCGGAGACT GGGGATCCTG TAGCGTCACG 
TGCGGCGATG GAGTCCAGAA GCGCGCCGTG GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA 
ACGATGGCGA AGCTTCCGGA CAAGGAGTGC ACAGGAATCC GACCGGCCGA ACTGCAACGT 
TGCACGATGA GACCGTGCGC CATGGACCAG AACCGCGAGG CTCTGACGGA GGAGGAACCA 
ACGGACCAAG GAGTCGTCAC AACGTATTCG TGGAGGAGCG CCGGTTACAC TCCGTGCACG 
GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA CTGGTCCACT GCGTTCGTAA TGAAGACGGA 
GTTCCTGTCA GCTACATCCA GTGTGACATC AGAGACAAGC CTGAAACAAT CACCCAGACC 
TGCAATAACC GGCCCTGTCC CCCAAGGTGG AACACGTCCG ACTTCACGGC GTGCAGCAAG 
CCGTGTGGCG GTGGTTCCCA GACGCGCACC GTGCAGTGCG TGCACGAGGT GACCCGGGGT 
GTCGGAAACA CCCTGGTGGT ACCAGACACC CACTGCCCGC TGCCACGCCC GGCCGAACAC 
CGCATGTGCA ACATGGTTGA CTGCGTCGCG CACTGGACTG CCGACAGGTG GGCCAAGTGC 
TCCAAGAGCT GTGACGGCGG GGTGAAGACG CGCAAGATCC ACTGTCAACG GGAACTGGCC 
TACGGAACGT TGGCGGACCT GCCGCCGTCA AAGTGTGCCT CGAAGAGAAA GCCTCGTACT 
GTGAAGCCCT GCAACACCAA GCCCTGCCGC AGGGGAAACC CCAGTTCAGT GCAGGGTGGA 
CCTAAGCGAA AGGTGTCCCT GATAGCCGAG GGAAAGGCTT CTGTCTTCCG AGGAACGACG 
CTCAAAATAC GCTGCCCAGG TGTCGTCGCC AACACTTCGT CTGACACAAG CAGTCGTGCC 
AAGTGGCTCA AGGACGGCCT CCCCGTCAAG ACCTCCTCGA GACGGGTGGT CGTGTCGAGA 
AAGGGAGGCC TGCGGATCAG GAAGGCGGAC TTCCCAGACG CCGGCACCTA CGTCTGCTCC 
ACGGGAACCT CCAAGAGCGA GCTCGTGCTT TCCATCCGAC CGCTCTCCTC GCCACCTCCA 
GACACTTCAC AAGGACTTCC CTTTGACGTG CTCAAGGGAC AGAAAGCAGA CGGTGGTACT 
TTTGCTTCGA AAGGTCACCA CCCCAACGGA GTTGGCTTGA AGCCAGCCAA AGGAGCCGAT 
CTGCCGTCGT ACATCAACAG TGTCGAAGAC GTGCAGACGA ACAAGGAACA AACTCTCTCC 
CAGGACACAA AGCTGGACCG AGCAACCTCC TCCGACGTGG ACCGGACCGC CTTCAAGGTG 
GACCTCGCTT CGGACCAAGA ACGCAACGAT CAAGGACTCC GAGAGGTGAA GGGGACCCTC 
GAAGATCCGA CAGTCAACTC GGGTGCATCT AGTCGGCCCG GTGGAAACAT TCCGCGCATC 
CAGACACTGC TGTCGGACTT TCACCGGCGG CTCGGCGGCA GTGGTGGTGG CAGAGACAGC 
GCGGTCAGGG GCACTGTCGA ACTCCCACCC GACGATGAGG ACCAGCTGGG CGTGCCCAAC 
AGCTACGTTC TCGGGCGTGG GAACGCAGAC AACCTCCAAT TCGAGTGGTC CACGACTCCC 
TGGTCCGCCT GCTCACAAAC GTGCGGAGGC AGCGGACTCC AGGTGCGTTC CACACAGTGC 
ATGGTGCGGC TCCACAACGT CTCTCGGACC GTGGACAGCA CGCTGTGCGC TGGTCTCGAA 
GCCCCCCAGA TCCTGCAGCG CTGCGGTTCG GCAGGCTGCC CACTCTGGGT CACTGGAAGC 
TGGTCCCAGT GTTCCGACGG CCGCTGCTTC ACGCTGAGCA CGTCGGTGCA AGAGCGCCTG 
GTGGAGTGCC GGCTTCCCGA CGGCACCCCA GTCGACGAGA AGTTGTGCGA CGCCTCGACG 
CGCCCGCAGG ACCAGCAGGA GTGCCTCAGC GAGCGCTGCC GCGGCGTCTG GAAGGTTGGA 
GACTGGTCAG AGTGCACGGC GACGTGCGGT GAGAGCGGTT TCCGGACGCG GATCCTGCGC 
TGCGTTTGGT ACGGCACCGA CAAGCCGGCC GGAGACTCCT GCCGCAATCA GGACCGGCCC 
GAAGTGCTTC AAGGCTGCGA TGCTCCCCCT TGCAACGCCA CCAGGCAGAC ATGCGAAGAC 
CACTCCATCA AGTGTCACAT GATCCGCCGC TTCAGCATGT GCCACATAAA CAAGTACCAT 
GACCTGTGCT GTGCCACCTG CGAGCGCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029829062.1
CDS	14..3526
Translation	MLPLLGRHRTLLLGTLAVALSWGTRCLHASSALDGETIADLIEAESDDGWSPWGPWSACSRTCDGGVTLQSRRCSRFGCVGESTRSRICNMQPCPEHVDFRATQCSEFDQVPYRGRLYEWTPVHDASDPCSLTCQAREFKFMAKLAPRAQDGTRCRQGALDMCVQGKCLPVGCDLELGSYLEVDECGICGGNGTSCRAPAFTWSETPFSECSVTCGGGFQESHPVCTSSDTGSEVDDRLCNVDERPDSRVRKCNGNPCPPGWQMGAWGDCSASCGGGQQVRNVSCVRPTVPPVPVAEHFCPGQRPATSQSCNLHDCSRWTEGQWSQCSATCGVGTQTRAVECRDLRGRKSHLCDPKRKPRSKQQCSAGNPCFSKGKEYGSQEDAPPQSFPPFNIFSKPQNQSPGSEPSFIVGDWGSCSVTCGDGVQKRAVECKIFLEFSRTMAKLPDKECTGIRPAELQRCTMRPCAMDQNREALTEEEPTDQGVVTTYSWRSAGYTPCTASCLGGIRESLVHCVRNEDGVPVSYIQCDIRDKPETITQTCNNRPCPPRWNTSDFTACSKPCGGGSQTRTVQCVHEVTRGVGNTLVVPDTHCPLPRPAEHRMCNMVDCVAHWTADRWAKCSKSCDGGVKTRKIHCQRELAYGTLADLPPSKCASKRKPRTVKPCNTKPCRRGNPSSVQGGPKRKVSLIAEGKASVFRGTTLKIRCPGVVANTSSDTSSRAKWLKDGLPVKTSSRRVVVSRKGGLRIRKADFPDAGTYVCSTGTSKSELVLSIRPLSSPPPDTSQGLPFDVLKGQKADGGTFASKGHHPNGVGLKPAKGADLPSYINSVEDVQTNKEQTLSQDTKLDRATSSDVDRTAFKVDLASDQERNDQGLREVKGTLEDPTVNSGASSRPGGNIPRIQTLLSDFHRRLGGSGGGRDSAVRGTVELPPDDEDQLGVPNSYVLGRGNADNLQFEWSTTPWSACSQTCGGSGLQVRSTQCMVRLHNVSRTVDSTLCAGLEAPQILQRCGSAGCPLWVTGSWSQCSDGRCFTLSTSVQERLVECRLPDGTPVDEKLCDASTRPQDQQECLSERCRGVWKVGDWSECTATCGESGFRTRILRCVWYGTDKPAGDSCRNQDRPEVLQGCDAPPCNATRQTCEDHSIKCHMIRRFSMCHINKYHDLCCATCERS

Target ORF information:

RefSeq Version	XM_029973202.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029973202.1

ORF Insert Sequence:

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGTG CTCCGCGACG TGTGGCGTGG GCACGCAGAC ACGGGCGGTG 
GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC CACCTGTGCG ACCCCAAGCG CAAGCCTCGG 
TCCAAGCAAC AGTGCTCCGC GGGAAACCCC TGCTTCTCCA AAGGTAAAGA GTATGGATCG 
CAAGAGGACG CACCACCCCA GAGTTTCCCG CCCTTCAACA TCTTCTCCAA ACCACAAAAC 
CAGTCACCGG GGTCGGAACC GAGCTTCATC GTCGGAGACT GGGGATCCTG TAGCGTCACG 
TGCGGCGATG GAGTCCAGAA GCGCGCCGTG GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA 
ACGATGGCGA AGCTTCCGGA CAAGGAGTGC ACAGGAATCC GACCGGCCGA ACTGCAACGT 
TGCACGATGA GACCGTGCGC CATGGACCAG AACCGCGAGG CTCTGACGGA GGAGGAACCA 
ACGGACCAAG GAGTCGTCAC AACGTATTCG TGGAGGAGCG CCGGTTACAC TCCGTGCACG 
GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA CTGGTCCACT GCGTTCGTAA TGAAGACGGA 
GTTCCTGTCA GCTACATCCA GTGTGACATC AGAGACAAGC CTGAAACAAT CACCCAGACC 
TGCAATAACC GGCCCTGTCC CCCAAGGTGG AACACGTCCG ACTTCACGGC GTGCAGCAAG 
CCGTGTGGCG GTGGTTCCCA GACGCGCACC GTGCAGTGCG TGCACGAGGT GACCCGGGGT 
GTCGGAAACA CCCTGGTGGT ACCAGACACC CACTGCCCGC TGCCACGCCC GGCCGAACAC 
CGCATGTGCA ACATGGTTGA CTGCGTCGCG CACTGGACTG CCGACAGGTG GGCCAAGTGC 
TCCAAGAGCT GTGACGGCGG GGTGAAGACG CGCAAGATCC ACTGTCAACG GGAACTGGCC 
TACGGAACGT TGGCGGACCT GCCGCCGTCA AAGTGTGCCT CGAAGAGAAA GCCTCGTACT 
GTGAAGCCCT GCAACACCAA GCCCTGCCGC AGGGGAAACC CCAGTTCAGT GCAGGGTGGA 
CCTAAGCGAA AGGTGTCCCT GATAGCCGAG GGAAAGGCTT CTGTCTTCCG AGGAACGACG 
CTCAAAATAC GCTGCCCAGG TGTCGTCGCC AACACTTCGT CTGACACAAG CAGTCGTGCC 
AAGTGGCTCA AGGACGGCCT CCCCGTCAAG ACCTCCTCGA GACGGGTGGT CGTGTCGAGA 
AAGGGAGGCC TGCGGATCAG GAAGGCGGAC TTCCCAGACG CCGGCACCTA CGTCTGCTCC 
ACGGGAACCT CCAAGAGCGA GCTCGTGCTT TCCATCCGAC CGCTCTCCTC GCCACCTCCA 
GACACTTCAC AAGGACTTCC CTTTGACGTG CTCAAGGGAC AGAAAGCAGA CGGTGGTACT 
TTTGCTTCGA AAGGTCACCA CCCCAACGGA GTTGGCTTGA AGCCAGCCAA AGGAGCCGAT 
CTGCCGTCGT ACATCAACAG TGTCGAAGAC GTGCAGACGA ACAAGGAACA AACTCTCTCC 
CAGGACACAA AGCTGGACCG AGCAACCTCC TCCGACGTGG ACCGGACCGC CTTCAAGGTG 
GACCTCGCTT CGGACCAAGA ACGCAACGAT CAAGGACTCC GAGAGGTGAA GGGGACCCTC 
GAAGATCCGA CAGTCAACTC GGGTGCATCT AGTCGGCCCG GTGGAAACAT TCCGCGCATC 
CAGACACTGC TGTCGGACTT TCACCGGCGG CTCGGCGGCA GTGGTGGTGG CAGAGACAGC 
GCGGTCAGGG GCACTGTCGA ACTCCCACCC GACGATGAGG ACCAGCTGGG CGTGCCCAAC 
AGCTACGTTC TCGGGCGTGG GAACGCAGAC AACCTCCAAT TCGAGTGGTC CACGACTCCC 
TGGTCCGCCT GCTCACAAAC GTGCGGAGGC AGCGGACTCC AGGTGCGTTC CACACAGTGC 
ATGGTGCGGC TCCACAACGT CTCTCGGACC GTGGACAGCA CGCTGTGCGC TGGTCTCGAA 
GCCCCCCAGA TCCTGCAGCG CTGCGGTTCG GCAGGCTGCC CACTCTGGGT CACTGGAAGC 
TGGTCCCAGT GTTCCGACGG CCGCTGCTTC ACGCTGAGCA CGTCGGTGCA AGAGCGCCTG 
GTGGAGTGCC GGCTTCCCGA CGGCACCCCA GTCGACGAGA AGTTGTGCGA CGCCTCGACG 
CGCCCGCAGG ACCAGCAGGA GTGCCTCAGC GAGCGCTGCC GCGGCGTCTG GAAGGTTGGA 
GACTGGTCAG AGTGCACGGC GACGTGCGGT GAGAGCGGTT TCCGGACGCG GATCCTGCGC 
TGCGTTTGGT ACGGCACCGA CAAGCCGGCC GGAGACTCCT GCCGCAATCA GGACCGGCCC 
GAAGTGCTTC AAGGCTGCGA TGCTCCCCCT TGCAACGCCA CCAGGCAGAC ATGCGAAGAC 
CACTCCATCA AGTGTCACAT GATCCGCCGC TTCAGCATGT GCCACATAAA CAAGTACCAT 
GACCTGTGCT GTGCCACCTG CGAGCGCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8036613 ( XM_029973203.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029829063.1 Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OIa42625
Clone ID Related Accession (Same CDS sequence)	XM_029973203.1
Accession Version	XM_029973203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	protein madd-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021924022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGAC ACGGGCGGTG GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC 
CACCTGTGCG ACCCCAAGCG CAAGCCTCGG TCCAAGCAAC AGTGCTCCGC GGGAAACCCC 
TGCTTCTCCA AAGGTAAAGA GTATGGATCG CAAGAGGACG CACCACCCCA GAGTTTCCCG 
CCCTTCAACA TCTTCTCCAA ACCACAAAAC CAGTCACCGG GGTCGGAACC GAGCTTCATC 
GTCGGAGACT GGGGATCCTG TAGCGTCACG TGCGGCGATG GAGTCCAGAA GCGCGCCGTG 
GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA ACGATGGCGA AGCTTCCGGA CAAGGAGTGC 
ACAGGAATCC GACCGGCCGA ACTGCAACGT TGCACGATGA GACCGTGCGC CATGGACCAG 
AACCGCGAGG CTCTGACGGA GGAGGAACCA ACGGACCAAG GAGTCGTCAC AACGTATTCG 
TGGAGGAGCG CCGGTTACAC TCCGTGCACG GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA 
CTGGTCCACT GCGTTCGTAA TGAAGACGGA GTTCCTGTCA GCTACATCCA GTGTGACATC 
AGAGACAAGC CTGAAACAAT CACCCAGACC TGCAATAACC GGCCCTGTCC CCCAAGGTGG 
AACACGTCCG ACTTCACGGC GTGCAGCAAG CCGTGTGGCG GTGGTTCCCA GACGCGCACC 
GTGCAGTGCG TGCACGAGGT GACCCGGGGT GTCGGAAACA CCCTGGTGGT ACCAGACACC 
CACTGCCCGC TGCCACGCCC GGCCGAACAC CGCATGTGCA ACATGGTTGA CTGCGTCGCG 
CACTGGACTG CCGACAGGTG GGCCAAGTGC TCCAAGAGCT GTGACGGCGG GGTGAAGACG 
CGCAAGATCC ACTGTCAACG GGAACTGGCC TACGGAACGT TGGCGGACCT GCCGCCGTCA 
AAGTGTGCCT CGAAGAGAAA GCCTCGTACT GTGAAGCCCT GCAACACCAA GCCCTGCCGC 
AGGGGAAACC CCAGTTCAGT GCAGGGTGGA CCTAAGCGAA AGGTGTCCCT GATAGCCGAG 
GGAAAGGCTT CTGTCTTCCG AGGAACGACG CTCAAAATAC GCTGCCCAGG TGTCGTCGCC 
AACACTTCGT CTGACACAAG CAGTCGTGCC AAGTGGCTCA AGGACGGCCT CCCCGTCAAG 
ACCTCCTCGA GACGGGTGGT CGTGTCGAGA AAGGGAGGCC TGCGGATCAG GAAGGCGGAC 
TTCCCAGACG CCGGCACCTA CGTCTGCTCC ACGGGAACCT CCAAGAGCGA GCTCGTGCTT 
TCCATCCGAC CGCTCTCCTC GCCACCTCCA GACACTTCAC AAGGACTTCC CTTTGACGTG 
CTCAAGGGAC AGAAAGGTTT GTCAACAGCA GACGGTGGTA CTTTTGCTTC GAAAGGTCAC 
CACCCCAACG GAGTTGGCTT GAAGCCAGCC AAAGGAGCCG ATCTGCCGTC GTACATCAAC 
AGTGTCGAAG ACGTGCAGAC GAACAAGGAA CAAACTCTCT CCCAGGACAC AAAGCTGGAC 
CGAGCAACCT CCTCCGACGT GGACCGGACC GCCTTCAAGG TGGACCTCGC TTCGGACCAA 
GAACGCAACG ATCAAGGACT CCGAGAGGTG AAGGGGACCC TCGAAGATCC GACAGTCAAC 
TCGGGTGCAT CTAGTCGGCC CGGTGGAAAC ATTCCGCGCA TCCAGACACT GCTGTCGGAC 
TTTCACCGGC GGCTCGGCGG CAGTGGTGGT GGCAGAGACA GCGCGGTCAG GGGCACTGTC 
GAACTCCCAC CCGACGATGA GGACCAGCTG GGCGTGCCCA ACAGCTACGT TCTCGGGCGT 
GGGAACGCAG ACAACCTCCA ATTCGAGTGG TCCACGACTC CCTGGTCCGC CTGCTCACAA 
ACGTGCGGAG GCAGCGGACT CCAGGTGCGT TCCACACAGT GCATGGTGCG GCTCCACAAC 
GTCTCTCGGA CCGTGGACAG CACGCTGTGC GCTGGTCTCG AAGCCCCCCA GATCCTGCAG 
CGCTGCGGTT CGGCAGGCTG CCCACTCTGG GTCACTGGAA GCTGGTCCCA GTGTTCCGAC 
GGCCGCTGCT TCACGCTGAG CACGTCGGTG CAAGAGCGCC TGGTGGAGTG CCGGCTTCCC 
GACGGCACCC CAGTCGACGA GAAGTTGTGC GACGCCTCGA CGCGCCCGCA GGACCAGCAG 
GAGTGCCTCA GCGAGCGCTG CCGCGGCGTC TGGAAGGTTG GAGACTGGTC AGAGTGCACG 
GCGACGTGCG GTGAGAGCGG TTTCCGGACG CGGATCCTGC GCTGCGTTTG GTACGGCACC 
GACAAGCCGG CCGGAGACTC CTGCCGCAAT CAGGACCGGC CCGAAGTGCT TCAAGGCTGC 
GATGCTCCCC CTTGCAACGC CACCAGGCAG ACATGCGAAG ACCACTCCAT CAAGTGTCAC 
ATGATCCGCC GCTTCAGCAT GTGCCACATA AACAAGTACC ATGACCTGTG CTGTGCCACC 
TGCGAGCGCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029829063.1
CDS	14..3508
Translation	MLPLLGRHRTLLLGTLAVALSWGTRCLHASSALDGETIADLIEAESDDGWSPWGPWSACSRTCDGGVTLQSRRCSRFGCVGESTRSRICNMQPCPEHVDFRATQCSEFDQVPYRGRLYEWTPVHDASDPCSLTCQAREFKFMAKLAPRAQDGTRCRQGALDMCVQGKCLPVGCDLELGSYLEVDECGICGGNGTSCRAPAFTWSETPFSECSVTCGGGFQESHPVCTSSDTGSEVDDRLCNVDERPDSRVRKCNGNPCPPGWQMGAWGDCSASCGGGQQVRNVSCVRPTVPPVPVAEHFCPGQRPATSQSCNLHDCSRWTEGQWSQTRAVECRDLRGRKSHLCDPKRKPRSKQQCSAGNPCFSKGKEYGSQEDAPPQSFPPFNIFSKPQNQSPGSEPSFIVGDWGSCSVTCGDGVQKRAVECKIFLEFSRTMAKLPDKECTGIRPAELQRCTMRPCAMDQNREALTEEEPTDQGVVTTYSWRSAGYTPCTASCLGGIRESLVHCVRNEDGVPVSYIQCDIRDKPETITQTCNNRPCPPRWNTSDFTACSKPCGGGSQTRTVQCVHEVTRGVGNTLVVPDTHCPLPRPAEHRMCNMVDCVAHWTADRWAKCSKSCDGGVKTRKIHCQRELAYGTLADLPPSKCASKRKPRTVKPCNTKPCRRGNPSSVQGGPKRKVSLIAEGKASVFRGTTLKIRCPGVVANTSSDTSSRAKWLKDGLPVKTSSRRVVVSRKGGLRIRKADFPDAGTYVCSTGTSKSELVLSIRPLSSPPPDTSQGLPFDVLKGQKGLSTADGGTFASKGHHPNGVGLKPAKGADLPSYINSVEDVQTNKEQTLSQDTKLDRATSSDVDRTAFKVDLASDQERNDQGLREVKGTLEDPTVNSGASSRPGGNIPRIQTLLSDFHRRLGGSGGGRDSAVRGTVELPPDDEDQLGVPNSYVLGRGNADNLQFEWSTTPWSACSQTCGGSGLQVRSTQCMVRLHNVSRTVDSTLCAGLEAPQILQRCGSAGCPLWVTGSWSQCSDGRCFTLSTSVQERLVECRLPDGTPVDEKLCDASTRPQDQQECLSERCRGVWKVGDWSECTATCGESGFRTRILRCVWYGTDKPAGDSCRNQDRPEVLQGCDAPPCNATRQTCEDHSIKCHMIRRFSMCHINKYHDLCCATCERS

Target ORF information:

RefSeq Version	XM_029973203.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029973203.1

ORF Insert Sequence:

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGAC ACGGGCGGTG GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC 
CACCTGTGCG ACCCCAAGCG CAAGCCTCGG TCCAAGCAAC AGTGCTCCGC GGGAAACCCC 
TGCTTCTCCA AAGGTAAAGA GTATGGATCG CAAGAGGACG CACCACCCCA GAGTTTCCCG 
CCCTTCAACA TCTTCTCCAA ACCACAAAAC CAGTCACCGG GGTCGGAACC GAGCTTCATC 
GTCGGAGACT GGGGATCCTG TAGCGTCACG TGCGGCGATG GAGTCCAGAA GCGCGCCGTG 
GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA ACGATGGCGA AGCTTCCGGA CAAGGAGTGC 
ACAGGAATCC GACCGGCCGA ACTGCAACGT TGCACGATGA GACCGTGCGC CATGGACCAG 
AACCGCGAGG CTCTGACGGA GGAGGAACCA ACGGACCAAG GAGTCGTCAC AACGTATTCG 
TGGAGGAGCG CCGGTTACAC TCCGTGCACG GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA 
CTGGTCCACT GCGTTCGTAA TGAAGACGGA GTTCCTGTCA GCTACATCCA GTGTGACATC 
AGAGACAAGC CTGAAACAAT CACCCAGACC TGCAATAACC GGCCCTGTCC CCCAAGGTGG 
AACACGTCCG ACTTCACGGC GTGCAGCAAG CCGTGTGGCG GTGGTTCCCA GACGCGCACC 
GTGCAGTGCG TGCACGAGGT GACCCGGGGT GTCGGAAACA CCCTGGTGGT ACCAGACACC 
CACTGCCCGC TGCCACGCCC GGCCGAACAC CGCATGTGCA ACATGGTTGA CTGCGTCGCG 
CACTGGACTG CCGACAGGTG GGCCAAGTGC TCCAAGAGCT GTGACGGCGG GGTGAAGACG 
CGCAAGATCC ACTGTCAACG GGAACTGGCC TACGGAACGT TGGCGGACCT GCCGCCGTCA 
AAGTGTGCCT CGAAGAGAAA GCCTCGTACT GTGAAGCCCT GCAACACCAA GCCCTGCCGC 
AGGGGAAACC CCAGTTCAGT GCAGGGTGGA CCTAAGCGAA AGGTGTCCCT GATAGCCGAG 
GGAAAGGCTT CTGTCTTCCG AGGAACGACG CTCAAAATAC GCTGCCCAGG TGTCGTCGCC 
AACACTTCGT CTGACACAAG CAGTCGTGCC AAGTGGCTCA AGGACGGCCT CCCCGTCAAG 
ACCTCCTCGA GACGGGTGGT CGTGTCGAGA AAGGGAGGCC TGCGGATCAG GAAGGCGGAC 
TTCCCAGACG CCGGCACCTA CGTCTGCTCC ACGGGAACCT CCAAGAGCGA GCTCGTGCTT 
TCCATCCGAC CGCTCTCCTC GCCACCTCCA GACACTTCAC AAGGACTTCC CTTTGACGTG 
CTCAAGGGAC AGAAAGGTTT GTCAACAGCA GACGGTGGTA CTTTTGCTTC GAAAGGTCAC 
CACCCCAACG GAGTTGGCTT GAAGCCAGCC AAAGGAGCCG ATCTGCCGTC GTACATCAAC 
AGTGTCGAAG ACGTGCAGAC GAACAAGGAA CAAACTCTCT CCCAGGACAC AAAGCTGGAC 
CGAGCAACCT CCTCCGACGT GGACCGGACC GCCTTCAAGG TGGACCTCGC TTCGGACCAA 
GAACGCAACG ATCAAGGACT CCGAGAGGTG AAGGGGACCC TCGAAGATCC GACAGTCAAC 
TCGGGTGCAT CTAGTCGGCC CGGTGGAAAC ATTCCGCGCA TCCAGACACT GCTGTCGGAC 
TTTCACCGGC GGCTCGGCGG CAGTGGTGGT GGCAGAGACA GCGCGGTCAG GGGCACTGTC 
GAACTCCCAC CCGACGATGA GGACCAGCTG GGCGTGCCCA ACAGCTACGT TCTCGGGCGT 
GGGAACGCAG ACAACCTCCA ATTCGAGTGG TCCACGACTC CCTGGTCCGC CTGCTCACAA 
ACGTGCGGAG GCAGCGGACT CCAGGTGCGT TCCACACAGT GCATGGTGCG GCTCCACAAC 
GTCTCTCGGA CCGTGGACAG CACGCTGTGC GCTGGTCTCG AAGCCCCCCA GATCCTGCAG 
CGCTGCGGTT CGGCAGGCTG CCCACTCTGG GTCACTGGAA GCTGGTCCCA GTGTTCCGAC 
GGCCGCTGCT TCACGCTGAG CACGTCGGTG CAAGAGCGCC TGGTGGAGTG CCGGCTTCCC 
GACGGCACCC CAGTCGACGA GAAGTTGTGC GACGCCTCGA CGCGCCCGCA GGACCAGCAG 
GAGTGCCTCA GCGAGCGCTG CCGCGGCGTC TGGAAGGTTG GAGACTGGTC AGAGTGCACG 
GCGACGTGCG GTGAGAGCGG TTTCCGGACG CGGATCCTGC GCTGCGTTTG GTACGGCACC 
GACAAGCCGG CCGGAGACTC CTGCCGCAAT CAGGACCGGC CCGAAGTGCT TCAAGGCTGC 
GATGCTCCCC CTTGCAACGC CACCAGGCAG ACATGCGAAG ACCACTCCAT CAAGTGTCAC 
ATGATCCGCC GCTTCAGCAT GTGCCACATA AACAAGTACC ATGACCTGTG CTGTGCCACC 
TGCGAGCGCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8036613 ( XM_029973204.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029829064.1 Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X4, mRNA.

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CloneID	OIa42626
Clone ID Related Accession (Same CDS sequence)	XM_029973204.1
Accession Version	XM_029973204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	protein madd-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021924022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGTG CTCCGCGACG TGTGGCGTGG GCACGCAGAC ACGGGCGGTG 
GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC CACCTGTGCG ACCCCAAGCG CAAGCCTCGG 
TCCAAGCAAC AGTGCTCCGC GGGAAACCCC TGCTTCTCCA AAGGTAAAGA GTATGGATCG 
CAAGAGGACG CACCACCCCA GAGTTTCCCG CCCTTCAACA TCTTCTCCAA ACCACAAAAC 
CAGTCACCGG GGTCGGAACC GAGCTTCATC GTCGGAGACT GGGGATCCTG TAGCGTCACG 
TGCGGCGATG GAGTCCAGAA GCGCGCCGTG GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA 
ACGATGGCGA AGCTTCCGGA CAAGGAGTGC ACAGGAATCC GACCGGCCGA ACTGCAACGT 
TGCACGATGA GACCGTGCGC CATGGACCAG AACCGCGAGG CTCTGACGGA GGAGGAACCA 
ACGGACCAAG GAGTCGTCAC AACGTATTCG TGGAGGAGCG CCGGTTACAC TCCGTGCACG 
GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA CTGGTCCACT GCGTTCGTAA TGAAGACGGA 
GTTCCTGTCA GCTACATCCA GTGTGACATC AGAGACAAGC CTGAAACAAT CACCCAGACC 
TGCAATAACC GGCCCTGTCC CCCAAGGTGG AACACGTCCG ACTTCACGGC GTGCAGCAAG 
CCGTGTGGCG GTGGTTCCCA GACGCGCACC GTGCAGTGCG TGCACGAGGT GACCCGGGGT 
GTCGGAAACA CCCTGGTGGT ACCAGACACC CACTGCCCGC TGCCACGCCC GGCCGAACAC 
CGCATGTGCA ACATGGTTGA CTGCGTCGCG CACTGGACTG CCGACAGGTG GGCCAAGTGC 
TCCAAGAGCT GTGACGGCGG GGTGAAGACG CGCAAGATCC ACTGTCAACG GGAACTGGCC 
TACGGAACGT TGGCGGACCT GCCGCCGTCA AAGTGTGCCT CGAAGAGAAA GCCTCGTACT 
GTGAAGCCCT GCAACACCAA GCCCTGCCGC AGGGGAAACC CCAGTTCAGT GCAGGGTGGA 
CCTAAGCGAA AGGTGTCCCT GATAGCCGAG GGAAAGGCTT CTGTCTTCCG AGGAACGACG 
CTCAAAATAC GCTGCCCAGG TGTCGTCGCC AACACTTCGT CTGACACAAG CAGTCGTGCC 
AAGTGGCTCA AGGACGGCCT CCCCGTCAAG ACCTCCTCGA GACGGGTGGT CGTGTCGAGA 
AAGGGAGGCC TGCGGATCAG GAAGGCGGAC TTCCCAGACG CCGGCACCTA CGTCTGCTCC 
ACGGGAACCT CCAAGAGCGA GCTCGTGCTT TCCATCCGAC CGCTCTCCTC GCCACCTCCA 
GACACTTCAC AAGGACTTCC CTTTGACGTG CTCAAGGGAC AGAAAGGTCA CCACCCCAAC 
GGAGTTGGCT TGAAGCCAGC CAAAGGAGCC GATCTGCCGT CGTACATCAA CAGTGTCGAA 
GACGTGCAGA CGAACAAGGA ACAAACTCTC TCCCAGGACA CAAAGCTGGA CCGAGCAACC 
TCCTCCGACG TGGACCGGAC CGCCTTCAAG GTGGACCTCG CTTCGGACCA AGAACGCAAC 
GATCAAGGAC TCCGAGAGGT GAAGGGGACC CTCGAAGATC CGACAGTCAA CTCGGGTGCA 
TCTAGTCGGC CCGGTGGAAA CATTCCGCGC ATCCAGACAC TGCTGTCGGA CTTTCACCGG 
CGGCTCGGCG GCAGTGGTGG TGGCAGAGAC AGCGCGGTCA GGGGCACTGT CGAACTCCCA 
CCCGACGATG AGGACCAGCT GGGCGTGCCC AACAGCTACG TTCTCGGGCG TGGGAACGCA 
GACAACCTCC AATTCGAGTG GTCCACGACT CCCTGGTCCG CCTGCTCACA AACGTGCGGA 
GGCAGCGGAC TCCAGGTGCG TTCCACACAG TGCATGGTGC GGCTCCACAA CGTCTCTCGG 
ACCGTGGACA GCACGCTGTG CGCTGGTCTC GAAGCCCCCC AGATCCTGCA GCGCTGCGGT 
TCGGCAGGCT GCCCACTCTG GGTCACTGGA AGCTGGTCCC AGTGTTCCGA CGGCCGCTGC 
TTCACGCTGA GCACGTCGGT GCAAGAGCGC CTGGTGGAGT GCCGGCTTCC CGACGGCACC 
CCAGTCGACG AGAAGTTGTG CGACGCCTCG ACGCGCCCGC AGGACCAGCA GGAGTGCCTC 
AGCGAGCGCT GCCGCGGCGT CTGGAAGGTT GGAGACTGGT CAGAGTGCAC GGCGACGTGC 
GGTGAGAGCG GTTTCCGGAC GCGGATCCTG CGCTGCGTTT GGTACGGCAC CGACAAGCCG 
GCCGGAGACT CCTGCCGCAA TCAGGACCGG CCCGAAGTGC TTCAAGGCTG CGATGCTCCC 
CCTTGCAACG CCACCAGGCA GACATGCGAA GACCACTCCA TCAAGTGTCA CATGATCCGC 
CGCTTCAGCA TGTGCCACAT AAACAAGTAC CATGACCTGT GCTGTGCCAC CTGCGAGCGC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029829064.1
CDS	14..3499
Translation	MLPLLGRHRTLLLGTLAVALSWGTRCLHASSALDGETIADLIEAESDDGWSPWGPWSACSRTCDGGVTLQSRRCSRFGCVGESTRSRICNMQPCPEHVDFRATQCSEFDQVPYRGRLYEWTPVHDASDPCSLTCQAREFKFMAKLAPRAQDGTRCRQGALDMCVQGKCLPVGCDLELGSYLEVDECGICGGNGTSCRAPAFTWSETPFSECSVTCGGGFQESHPVCTSSDTGSEVDDRLCNVDERPDSRVRKCNGNPCPPGWQMGAWGDCSASCGGGQQVRNVSCVRPTVPPVPVAEHFCPGQRPATSQSCNLHDCSRWTEGQWSQCSATCGVGTQTRAVECRDLRGRKSHLCDPKRKPRSKQQCSAGNPCFSKGKEYGSQEDAPPQSFPPFNIFSKPQNQSPGSEPSFIVGDWGSCSVTCGDGVQKRAVECKIFLEFSRTMAKLPDKECTGIRPAELQRCTMRPCAMDQNREALTEEEPTDQGVVTTYSWRSAGYTPCTASCLGGIRESLVHCVRNEDGVPVSYIQCDIRDKPETITQTCNNRPCPPRWNTSDFTACSKPCGGGSQTRTVQCVHEVTRGVGNTLVVPDTHCPLPRPAEHRMCNMVDCVAHWTADRWAKCSKSCDGGVKTRKIHCQRELAYGTLADLPPSKCASKRKPRTVKPCNTKPCRRGNPSSVQGGPKRKVSLIAEGKASVFRGTTLKIRCPGVVANTSSDTSSRAKWLKDGLPVKTSSRRVVVSRKGGLRIRKADFPDAGTYVCSTGTSKSELVLSIRPLSSPPPDTSQGLPFDVLKGQKGHHPNGVGLKPAKGADLPSYINSVEDVQTNKEQTLSQDTKLDRATSSDVDRTAFKVDLASDQERNDQGLREVKGTLEDPTVNSGASSRPGGNIPRIQTLLSDFHRRLGGSGGGRDSAVRGTVELPPDDEDQLGVPNSYVLGRGNADNLQFEWSTTPWSACSQTCGGSGLQVRSTQCMVRLHNVSRTVDSTLCAGLEAPQILQRCGSAGCPLWVTGSWSQCSDGRCFTLSTSVQERLVECRLPDGTPVDEKLCDASTRPQDQQECLSERCRGVWKVGDWSECTATCGESGFRTRILRCVWYGTDKPAGDSCRNQDRPEVLQGCDAPPCNATRQTCEDHSIKCHMIRRFSMCHINKYHDLCCATCERS

Target ORF information:

RefSeq Version	XM_029973204.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis protein madd-4 (LOC8036613), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029973204.1

ORF Insert Sequence:

ATGCTACCGC TGCTGGGACG GCACCGGACG CTACTCTTGG GAACGTTAGC GGTGGCGCTG 
TCCTGGGGTA CGCGATGTCT CCACGCTTCC TCTGCACTGG ATGGAGAGAC TATCGCGGAC 
CTAATCGAAG CGGAGTCTGA CGACGGCTGG TCTCCCTGGG GGCCGTGGAG CGCGTGCTCC 
CGCACGTGCG ACGGCGGCGT CACCCTCCAG TCCCGCCGGT GCTCCAGGTT CGGATGCGTG 
GGCGAGAGCA CGCGCTCGCG CATCTGCAAC ATGCAGCCGT GCCCGGAGCA CGTGGACTTC 
AGGGCCACGC AGTGCTCGGA GTTCGACCAG GTGCCCTACC GGGGACGCCT GTACGAGTGG 
ACGCCTGTGC ACGACGCCAG CGACCCGTGC AGCCTTACCT GCCAGGCCAG GGAGTTCAAG 
TTCATGGCCA AGCTGGCGCC CCGGGCCCAG GACGGCACGC GCTGTCGCCA GGGGGCCCTC 
GACATGTGCG TCCAGGGAAA GTGCCTGCCC GTCGGCTGTG ACTTGGAGCT CGGCTCCTAC 
CTCGAGGTGG ACGAGTGCGG CATCTGTGGA GGCAACGGGA CGTCCTGCCG AGCACCTGCC 
TTCACCTGGT CCGAGACGCC CTTCTCGGAG TGCTCTGTGA CGTGTGGTGG CGGCTTCCAG 
GAGAGTCATC CTGTCTGCAC GTCTTCAGAC ACTGGTTCCG AAGTAGACGA CCGGTTGTGC 
AATGTCGACG AGAGGCCCGA CTCTCGGGTC CGGAAGTGCA ATGGCAATCC CTGTCCCCCT 
GGGTGGCAGA TGGGCGCTTG GGGCGACTGC AGCGCCTCGT GCGGCGGCGG CCAGCAGGTG 
CGCAACGTAT CATGCGTGCG CCCCACGGTG CCCCCTGTTC CGGTGGCAGA GCACTTCTGC 
CCCGGCCAGC GACCCGCAAC GTCCCAAAGC TGCAACCTCC ACGACTGTTC CCGCTGGACG 
GAGGGCCAGT GGTCACAGTG CTCCGCGACG TGTGGCGTGG GCACGCAGAC ACGGGCGGTG 
GAGTGCCGTG ACCTCCGCGG CCGCAAGAGC CACCTGTGCG ACCCCAAGCG CAAGCCTCGG 
TCCAAGCAAC AGTGCTCCGC GGGAAACCCC TGCTTCTCCA AAGGTAAAGA GTATGGATCG 
CAAGAGGACG CACCACCCCA GAGTTTCCCG CCCTTCAACA TCTTCTCCAA ACCACAAAAC 
CAGTCACCGG GGTCGGAACC GAGCTTCATC GTCGGAGACT GGGGATCCTG TAGCGTCACG 
TGCGGCGATG GAGTCCAGAA GCGCGCCGTG GAGTGCAAGA TCTTCCTGGA GTTCTCCAGA 
ACGATGGCGA AGCTTCCGGA CAAGGAGTGC ACAGGAATCC GACCGGCCGA ACTGCAACGT 
TGCACGATGA GACCGTGCGC CATGGACCAG AACCGCGAGG CTCTGACGGA GGAGGAACCA 
ACGGACCAAG GAGTCGTCAC AACGTATTCG TGGAGGAGCG CCGGTTACAC TCCGTGCACG 
GCGTCTTGCC TTGGAGGTAT TAGAGAGTCA CTGGTCCACT GCGTTCGTAA TGAAGACGGA 
GTTCCTGTCA GCTACATCCA GTGTGACATC AGAGACAAGC CTGAAACAAT CACCCAGACC 
TGCAATAACC GGCCCTGTCC CCCAAGGTGG AACACGTCCG ACTTCACGGC GTGCAGCAAG 
CCGTGTGGCG GTGGTTCCCA GACGCGCACC GTGCAGTGCG TGCACGAGGT GACCCGGGGT 
GTCGGAAACA CCCTGGTGGT ACCAGACACC CACTGCCCGC TGCCACGCCC GGCCGAACAC 
CGCATGTGCA ACATGGTTGA CTGCGTCGCG CACTGGACTG CCGACAGGTG GGCCAAGTGC 
TCCAAGAGCT GTGACGGCGG GGTGAAGACG CGCAAGATCC ACTGTCAACG GGAACTGGCC 
TACGGAACGT TGGCGGACCT GCCGCCGTCA AAGTGTGCCT CGAAGAGAAA GCCTCGTACT 
GTGAAGCCCT GCAACACCAA GCCCTGCCGC AGGGGAAACC CCAGTTCAGT GCAGGGTGGA 
CCTAAGCGAA AGGTGTCCCT GATAGCCGAG GGAAAGGCTT CTGTCTTCCG AGGAACGACG 
CTCAAAATAC GCTGCCCAGG TGTCGTCGCC AACACTTCGT CTGACACAAG CAGTCGTGCC 
AAGTGGCTCA AGGACGGCCT CCCCGTCAAG ACCTCCTCGA GACGGGTGGT CGTGTCGAGA 
AAGGGAGGCC TGCGGATCAG GAAGGCGGAC TTCCCAGACG CCGGCACCTA CGTCTGCTCC 
ACGGGAACCT CCAAGAGCGA GCTCGTGCTT TCCATCCGAC CGCTCTCCTC GCCACCTCCA 
GACACTTCAC AAGGACTTCC CTTTGACGTG CTCAAGGGAC AGAAAGGTCA CCACCCCAAC 
GGAGTTGGCT TGAAGCCAGC CAAAGGAGCC GATCTGCCGT CGTACATCAA CAGTGTCGAA 
GACGTGCAGA CGAACAAGGA ACAAACTCTC TCCCAGGACA CAAAGCTGGA CCGAGCAACC 
TCCTCCGACG TGGACCGGAC CGCCTTCAAG GTGGACCTCG CTTCGGACCA AGAACGCAAC 
GATCAAGGAC TCCGAGAGGT GAAGGGGACC CTCGAAGATC CGACAGTCAA CTCGGGTGCA 
TCTAGTCGGC CCGGTGGAAA CATTCCGCGC ATCCAGACAC TGCTGTCGGA CTTTCACCGG 
CGGCTCGGCG GCAGTGGTGG TGGCAGAGAC AGCGCGGTCA GGGGCACTGT CGAACTCCCA 
CCCGACGATG AGGACCAGCT GGGCGTGCCC AACAGCTACG TTCTCGGGCG TGGGAACGCA 
GACAACCTCC AATTCGAGTG GTCCACGACT CCCTGGTCCG CCTGCTCACA AACGTGCGGA 
GGCAGCGGAC TCCAGGTGCG TTCCACACAG TGCATGGTGC GGCTCCACAA CGTCTCTCGG 
ACCGTGGACA GCACGCTGTG CGCTGGTCTC GAAGCCCCCC AGATCCTGCA GCGCTGCGGT 
TCGGCAGGCT GCCCACTCTG GGTCACTGGA AGCTGGTCCC AGTGTTCCGA CGGCCGCTGC 
TTCACGCTGA GCACGTCGGT GCAAGAGCGC CTGGTGGAGT GCCGGCTTCC CGACGGCACC 
CCAGTCGACG AGAAGTTGTG CGACGCCTCG ACGCGCCCGC AGGACCAGCA GGAGTGCCTC 
AGCGAGCGCT GCCGCGGCGT CTGGAAGGTT GGAGACTGGT CAGAGTGCAC GGCGACGTGC 
GGTGAGAGCG GTTTCCGGAC GCGGATCCTG CGCTGCGTTT GGTACGGCAC CGACAAGCCG 
GCCGGAGACT CCTGCCGCAA TCAGGACCGG CCCGAAGTGC TTCAAGGCTG CGATGCTCCC 
CCTTGCAACG CCACCAGGCA GACATGCGAA GACCACTCCA TCAAGTGTCA CATGATCCGC 
CGCTTCAGCA TGTGCCACAT AAACAAGTAC CATGACCTGT GCTGTGCCAC CTGCGAGCGC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8036613 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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