LOC8031915 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8031915
Entrez Gene ID	8031915
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002407810.1	XP_002407854.1	hypothetical protein
XM_029990793.1	XP_029846653.1	uncharacterized protein LOC8031915 isoform X2
XM_029990788.1	XP_029846648.1	uncharacterized protein LOC8031915 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC8031915 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8031915 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa04942	XM_002407810.1 Latest version!	Ixodes scapularis conserved hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OIa16172	XM_029990788.1 Latest version!	Ixodes scapularis uncharacterized LOC8031915 (LOC8031915), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OIa16173	XM_029990793.1 Latest version!	Ixodes scapularis uncharacterized LOC8031915 (LOC8031915), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8031915 ( XM_002407810.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002407854.1 Ixodes scapularis conserved hypothetical protein, mRNA.

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CloneID	OIa04942
Clone ID Related Accession (Same CDS sequence)	XM_002407810.1
Accession Version	XM_002407810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002692652). COMPLETENESS: incomplete on the 5' end.

ATGCACTGCC CCGTCGACTT GGGAGTGCTC ACGGTATGGG GCGACCTTGG TGTGCAGAGC 
CAGAGCCTGC GAGACATGGA GCGGGAGCTG GTGTGGAATC TGTCGGATGC CCAGGGCAAC 
AGCCTGTGGC ACGTGTGCGC GGCCCGGAAC CATCTGCGCT GCTTCCAGTG GCTCTGCCAG 
GGACACCCAC AGCACGTCCT GGCTGACGAG AACCACAGCA GCCTCACGCC CGTCGGAACT 
GCCGTCAAGC ATGGGAACCT GGAGATAGTC CAATGGCTCG TCAGCAAGTC CTGGGCCCTG 
GAACAAATTA ACCCTCCAGA GGGGAACAGG ACCTTGCTTC ATCTAGCTGC AAAGTATGGT 
CAGGAGAGAG TGGTGCGTTG GCTGGCAGAG TATATGCAGA ACAACAACCT GAACATCAAC 
CGGAAGGACA ATGACGGAAA CACTCCGGTG CATCTGGCTG CGAAACATGG ACACATCTCC 
GTCATCAAGA CCCTGGTGCT GTTGGATGCG GACGTGACGG CTCAGAACGA GCAAGGCCTG 
CGGCCGCATG GGGTGGCCGT GCAGAGTGGC CAGGTGGCCT GTGCGGACCA CCTGCTCACC 
ACCGAGGTCT GCCTCTGTCT CTCCTCTGAG CAGGTTCTCA TCGAGGGAAA CTTCCAGAGG 
TCCCACTTTG CCCAAGCCCT GGCGAAGTGC AGCGGCCCCC ACGTCCAACC GGAAGCCAGG 
CCCGCCTCCT CGTCCTCCCT GTCGGACTAC AGTGGGGAGG AGAGTGCAGA GGAGGAGCCC 
CAGCGGCCAC CTGCCAGCGC CCGGCGTGTC CACGAGCAGG TTCCAGCTCC CTGGGAGCAG 
GAAGTGCGCT CCTGGGACAA GGTCCAGGTG TTCCTCGATG GCCTGCTGCG CAACAGCGAC 
ATCCAGCGCA GTAACAACAA CCCAACGCCC ACGAGGAAGG CCAACAACTC GACCAACACA 
CTCGTCATCC GCCACGGCAC AAGCGGTCGC AAGTGTCGAG TCAAGGCGGC GCAGAGCCGA 
AAAGTGGAAG CGCCCAAGGA CAGGTCTCAG GAAAACGTGA GCCTGAAGGT GCTTTTCGAA 
AACAACCCGG AACTGCGGCA GGAAGGTCAG ACTTGTTCGG TGCTTGAGGT CTTGGAGCCC 
TCGAGCAGCG ATGCAGAAGA AGACCTCAAC GTGGGACTGC GGCGCGGAAG CGGGGACAGC 
TCGTCGAGCT CCTTCCGCTC TAACGAGAAT GCGGTGCTTC ACGTGGCGGA GCAGCGGTGT 
CCCAAGAAAC CCGTAGCTGC GAGCAATGGC GAGCGCAAGG GCAAGGGCGC CATCCTTCTG 
ACCAACATCG CAGAGCTGCC CGATTGCTCA AGCAGGTACA AGGTCAGCTT CAAGATCCAG 
CAGTCGCAGG AATGCCCTCA CCTCGGCTCG GTAGAGACGC AGCCCAAGGA TGAAGCAGAC 
GCGGCAAATG ACAAGAAGGG CAGAAACGCA GAGCGGCAGA TGAGTTTCGC AGAGCCCGAC 
TTGATTCGGG GGACCAACGT GGCAGAACGT TGCGCCCCGG AGCGCAGCAC CGCGGAGGCC 
TCTTCGGCGG GCGAGTGCCC CTCGGAGCTG TCCTTCGGAG ACTCGGCCAG CCAGGACGTC 
ACGGGGACGG AGGACGTGGA GGAGCAGCAG CATGCGGCGC AGGGTCTCTC ATCCCCAGAG 
TGCTCCGTGC CAGCAGGCGA AACCTCTTCC GGGGCGGGAA GTGCCAAAAA GCGCAGCGGA 
TTTCTGCTCA AGTTCTCGCT AAGGGGCCGC TGGCCGTCCA AACAGAAGCA GCAGCGGAAG 
AGCGAGGAGA TCAGCGCGGA GGAGTTTCGA GAAACATACA CGCGGAGTGC GGGGGCGGAG 
CCCTTCAGCC GGGACCCCCT TCCGGAGCAA AGCACCACGG CCAAAGCGAG CGAGCTGTTT 
GCGCTCTCAC CGCCCCCAAT CCCGACGCTT CCAAGGCGGG GTCCTCCCCC TGTGCCCCCG 
TGCGAGGAGG AGGTGCCGCC CGAGTCCCCC GAGCCCTCCC TGGGGAGCAA GTGCTCCCCG 
ACCGAGGACG AGGCCCTACT CTACTTGCAT GGGGGGTCGC CAGAGCCCAG CATCTTGAGG 
CCAGATTCCA TCGTCTCTAA GACCACCGGA GGCTCCCTGT CCCGCCCGGC GTCCTCGGCG 
TCCCAGACGG AGGACGGGAG GCGGGGTGCC AGGGAAGGGG CGGGCCGGGG GAATGTGGCC 
GTGGTGGGCT TCTCCCTGCC CAAGACGCTA TCCTCCACTG AGGTGTTGCT GCTGCACACA 
CCGGAGTCCT CCGTGGCGGG GCGGCAGTCG CCTGCCCCCA GCGAGGTCTC CAAGACGGAG 
AGCGCCCTGT CACCGCCCAG CGACGTGTCC AAGACGGAGA GTGCGAGGCA GGCACCACCC 
CTGGGAAAAA TCGAGGAAAT TGTGGCGGCC GCGGGGGCCG CCGCTGCTGC ATCAAGCGCG 
GAGAAGAGGG TGGACCCGTC GGCCGCTGCC CTCAGGGCCC GGCCTTCCGC GGGTGTGGAG 
GCCCCCAAGC AAGAGGGCCA TCCCTGCATG ACCCCCGTGG CCATTCTGCA GCAGGACGTC 
CCGCCGCATC AGGCAAAGGC CAAAAAGATG ACATTGGCTG CTAGGAAAAA CAAGAAAAGT 
TCAAAGCCCT GGTACGAGGT TTCGGACGAG GAGGACATGC TGTCTCCCGA CAAGTACCAG 
ACAGTCAGGG CTCGCAGCAG CTCCGAAGAC GAGACAGACC TGGCTGTTGT GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002407854.1
CDS	1..2757
Translation	MHCPVDLGVLTVWGDLGVQSQSLRDMERELVWNLSDAQGNSLWHVCAARNHLRCFQWLCQGHPQHVLADENHSSLTPVGTAVKHGNLEIVQWLVSKSWALEQINPPEGNRTLLHLAAKYGQERVVRWLAEYMQNNNLNINRKDNDGNTPVHLAAKHGHISVIKTLVLLDADVTAQNEQGLRPHGVAVQSGQVACADHLLTTEVCLCLSSEQVLIEGNFQRSHFAQALAKCSGPHVQPEARPASSSSLSDYSGEESAEEEPQRPPASARRVHEQVPAPWEQEVRSWDKVQVFLDGLLRNSDIQRSNNNPTPTRKANNSTNTLVIRHGTSGRKCRVKAAQSRKVEAPKDRSQENVSLKVLFENNPELRQEGQTCSVLEVLEPSSSDAEEDLNVGLRRGSGDSSSSSFRSNENAVLHVAEQRCPKKPVAASNGERKGKGAILLTNIAELPDCSSRYKVSFKIQQSQECPHLGSVETQPKDEADAANDKKGRNAERQMSFAEPDLIRGTNVAERCAPERSTAEASSAGECPSELSFGDSASQDVTGTEDVEEQQHAAQGLSSPECSVPAGETSSGAGSAKKRSGFLLKFSLRGRWPSKQKQQRKSEEISAEEFRETYTRSAGAEPFSRDPLPEQSTTAKASELFALSPPPIPTLPRRGPPPVPPCEEEVPPESPEPSLGSKCSPTEDEALLYLHGGSPEPSILRPDSIVSKTTGGSLSRPASSASQTEDGRRGAREGAGRGNVAVVGFSLPKTLSSTEVLLLHTPESSVAGRQSPAPSEVSKTESALSPPSDVSKTESARQAPPLGKIEEIVAAAGAAAAASSAEKRVDPSAAALRARPSAGVEAPKQEGHPCMTPVAILQQDVPPHQAKAKKMTLAARKNKKSSKPWYEVSDEEDMLSPDKYQTVRARSSSEDETDLAVVA

Target ORF information:

RefSeq Version	XM_002407810.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis conserved hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002407810.1

ORF Insert Sequence:

ATGCACTGCC CCGTCGACTT GGGAGTGCTC ACGGTATGGG GCGACCTTGG TGTGCAGAGC 
CAGAGCCTGC GAGACATGGA GCGGGAGCTG GTGTGGAATC TGTCGGATGC CCAGGGCAAC 
AGCCTGTGGC ACGTGTGCGC GGCCCGGAAC CATCTGCGCT GCTTCCAGTG GCTCTGCCAG 
GGACACCCAC AGCACGTCCT GGCTGACGAG AACCACAGCA GCCTCACGCC CGTCGGAACT 
GCCGTCAAGC ATGGGAACCT GGAGATAGTC CAATGGCTCG TCAGCAAGTC CTGGGCCCTG 
GAACAAATTA ACCCTCCAGA GGGGAACAGG ACCTTGCTTC ATCTAGCTGC AAAGTATGGT 
CAGGAGAGAG TGGTGCGTTG GCTGGCAGAG TATATGCAGA ACAACAACCT GAACATCAAC 
CGGAAGGACA ATGACGGAAA CACTCCGGTG CATCTGGCTG CGAAACATGG ACACATCTCC 
GTCATCAAGA CCCTGGTGCT GTTGGATGCG GACGTGACGG CTCAGAACGA GCAAGGCCTG 
CGGCCGCATG GGGTGGCCGT GCAGAGTGGC CAGGTGGCCT GTGCGGACCA CCTGCTCACC 
ACCGAGGTCT GCCTCTGTCT CTCCTCTGAG CAGGTTCTCA TCGAGGGAAA CTTCCAGAGG 
TCCCACTTTG CCCAAGCCCT GGCGAAGTGC AGCGGCCCCC ACGTCCAACC GGAAGCCAGG 
CCCGCCTCCT CGTCCTCCCT GTCGGACTAC AGTGGGGAGG AGAGTGCAGA GGAGGAGCCC 
CAGCGGCCAC CTGCCAGCGC CCGGCGTGTC CACGAGCAGG TTCCAGCTCC CTGGGAGCAG 
GAAGTGCGCT CCTGGGACAA GGTCCAGGTG TTCCTCGATG GCCTGCTGCG CAACAGCGAC 
ATCCAGCGCA GTAACAACAA CCCAACGCCC ACGAGGAAGG CCAACAACTC GACCAACACA 
CTCGTCATCC GCCACGGCAC AAGCGGTCGC AAGTGTCGAG TCAAGGCGGC GCAGAGCCGA 
AAAGTGGAAG CGCCCAAGGA CAGGTCTCAG GAAAACGTGA GCCTGAAGGT GCTTTTCGAA 
AACAACCCGG AACTGCGGCA GGAAGGTCAG ACTTGTTCGG TGCTTGAGGT CTTGGAGCCC 
TCGAGCAGCG ATGCAGAAGA AGACCTCAAC GTGGGACTGC GGCGCGGAAG CGGGGACAGC 
TCGTCGAGCT CCTTCCGCTC TAACGAGAAT GCGGTGCTTC ACGTGGCGGA GCAGCGGTGT 
CCCAAGAAAC CCGTAGCTGC GAGCAATGGC GAGCGCAAGG GCAAGGGCGC CATCCTTCTG 
ACCAACATCG CAGAGCTGCC CGATTGCTCA AGCAGGTACA AGGTCAGCTT CAAGATCCAG 
CAGTCGCAGG AATGCCCTCA CCTCGGCTCG GTAGAGACGC AGCCCAAGGA TGAAGCAGAC 
GCGGCAAATG ACAAGAAGGG CAGAAACGCA GAGCGGCAGA TGAGTTTCGC AGAGCCCGAC 
TTGATTCGGG GGACCAACGT GGCAGAACGT TGCGCCCCGG AGCGCAGCAC CGCGGAGGCC 
TCTTCGGCGG GCGAGTGCCC CTCGGAGCTG TCCTTCGGAG ACTCGGCCAG CCAGGACGTC 
ACGGGGACGG AGGACGTGGA GGAGCAGCAG CATGCGGCGC AGGGTCTCTC ATCCCCAGAG 
TGCTCCGTGC CAGCAGGCGA AACCTCTTCC GGGGCGGGAA GTGCCAAAAA GCGCAGCGGA 
TTTCTGCTCA AGTTCTCGCT AAGGGGCCGC TGGCCGTCCA AACAGAAGCA GCAGCGGAAG 
AGCGAGGAGA TCAGCGCGGA GGAGTTTCGA GAAACATACA CGCGGAGTGC GGGGGCGGAG 
CCCTTCAGCC GGGACCCCCT TCCGGAGCAA AGCACCACGG CCAAAGCGAG CGAGCTGTTT 
GCGCTCTCAC CGCCCCCAAT CCCGACGCTT CCAAGGCGGG GTCCTCCCCC TGTGCCCCCG 
TGCGAGGAGG AGGTGCCGCC CGAGTCCCCC GAGCCCTCCC TGGGGAGCAA GTGCTCCCCG 
ACCGAGGACG AGGCCCTACT CTACTTGCAT GGGGGGTCGC CAGAGCCCAG CATCTTGAGG 
CCAGATTCCA TCGTCTCTAA GACCACCGGA GGCTCCCTGT CCCGCCCGGC GTCCTCGGCG 
TCCCAGACGG AGGACGGGAG GCGGGGTGCC AGGGAAGGGG CGGGCCGGGG GAATGTGGCC 
GTGGTGGGCT TCTCCCTGCC CAAGACGCTA TCCTCCACTG AGGTGTTGCT GCTGCACACA 
CCGGAGTCCT CCGTGGCGGG GCGGCAGTCG CCTGCCCCCA GCGAGGTCTC CAAGACGGAG 
AGCGCCCTGT CACCGCCCAG CGACGTGTCC AAGACGGAGA GTGCGAGGCA GGCACCACCC 
CTGGGAAAAA TCGAGGAAAT TGTGGCGGCC GCGGGGGCCG CCGCTGCTGC ATCAAGCGCG 
GAGAAGAGGG TGGACCCGTC GGCCGCTGCC CTCAGGGCCC GGCCTTCCGC GGGTGTGGAG 
GCCCCCAAGC AAGAGGGCCA TCCCTGCATG ACCCCCGTGG CCATTCTGCA GCAGGACGTC 
CCGCCGCATC AGGCAAAGGC CAAAAAGATG ACATTGGCTG CTAGGAAAAA CAAGAAAAGT 
TCAAAGCCCT GGTACGAGGT TTCGGACGAG GAGGACATGC TGTCTCCCGA CAAGTACCAG 
ACAGTCAGGG CTCGCAGCAG CTCCGAAGAC GAGACAGACC TGGCTGTTGT GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8031915 ( XM_029990788.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029846648.1 Ixodes scapularis uncharacterized LOC8031915 (LOC8031915), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa16172
Clone ID Related Accession (Same CDS sequence)	XM_029990788.1
Accession Version	XM_029990788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8031915 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021917938.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCCC AGCTGCAGGG GCCGCCCACG GCGCCCAGGC AGCGCCCGCT CCTGCACCAG 
GTCCCGTGGG GAGTGTCCCA GAACGACCTT GAAGCCGTCG AGGACATTCT GTCGGCCACG 
TCACTCGTCG CATCGCCTGT GCGTCCGGGC GTGCTGTCGT CTCGGCGGAG TCGTGCCGGA 
GCTGCGGGCC ATGGGGCATC CTGTGGGGGC TGCCCGATCA CGGACACGAC ACGAGCGCCC 
AGGGCCGCCG CTCTTACCGC ACAGCCTGCA GCGGCGGGTC CCCGAGCGGC GGCCAAGCCT 
CGGCGCCCCG GCGCCCAGCA AGCCCCGGCT TGGGCGGACC CCAGCCGCGG CACCGGCAGC 
AGCAGCGGCG ACGTCTTGAG CGGCGCCGCC GCCATGCGGC ACCCGGACGA CGGCGGGGCC 
GTCGAGGAGT TCGTCCTCGA CGACCGAGAC CTGCTGCGCC TCTGCGAGGT ACCGCTGCAG 
CGCAGCAGGA CCGCCGCGGC GGGTCCCCGG CGGCCGCACC TGCGCACGGT CTCCATCATC 
GACACCCTGA GCGCGCTGGC CCGGGGCGCC GCCGACCGGG ACGTGTTGCC GCCGCCGCCA 
CGGGACCCCT GGGCGCGCGG TGCGGCGCCG CCGCCGCCGC CGTACGCGCG GCGCACGGTC 
CAGGCCGGGG GCGGGTCGCA CTGGCGGCGG TCGCGGCGGC AGGAGCGCGA CTGGTCGCGG 
CAGTGCGCCT CGGACAGCGA GGTGAAGGGC GGTGGCGACG CGTGCTCGGT GCTGTCGCTG 
CGCAGCCAGC CGCCCCGGAG GAGCAGCGCC CAGGAGCGGC CGGCCGCTGC CAGGGTGCCC 
AGAGTGCCCA ACTGGATATG CGCCATCTTC AGGCTCGCCA AGAAGGGGGA CCTCGAGGCT 
CTGAGCCAGA GCCTGCGAGA CATGGAGCGG GAGCTGGTTT GGAACCTGTC AGATGCCCAG 
GGCAACAGCC TGTGGCACGT GTGCGCGGCC CGGAACCACC TGCGCTGCTT CCAGTGGCTC 
TGCCAGGGAC ACCCACAGCA CGTCCTGGCT GACGAGAACC ACAGCAGCCT CACGCCCGTC 
GGAACTGCCG TCAAGCATGG GAACCTGGAG ATAGTCCAAT GGCTCGTCAG CAAGTCCTGG 
GCCCTGGAAC AAATTAACCC TCCAGAGGGG AACAGGACCT TGCTTCATCT AGCTGCAAAG 
TATGGTCAGG AGAGAGTGGT GCGTTGGCTG GCAGAGTATA TGCAGAACAA CAACCTGAAC 
ATCAACCGGA AGGACAATGA CGGAAACACT CCGGTGCATC TGGCTGCGAA ACATGGACAC 
ATCTCCGTCA TCAAGACCCT GGTGCTGTTG GATGCGGACG TGACGGCTCA GAACGAGCAA 
GGCCTGCGGC CGCATGGGGT GGCCGTGCAG AGTGGCCAGG TGGCCTGTGC GGACCACCTG 
CTCACCACCG AGGTCTGCCT CTGTCTCTCC TCTGAGCAGG TTCTCATCGA GGGAAACTTC 
CAGAGCTTGC AAGTTGAAAA TGCTGAGATG AAGAATGGCT TCAGAGAGCT GCTGGTGCTT 
ACCAAGAGGC TGTTGAGGAG ACAGGAGGAG ATGCTACACT ATGTGCAAGT GGTCTGTTCG 
GGAAACATGA ACTCGGACAA GGCGGAGTCG GACAGTGCAG ACTCACTTCC GTGGAACTCC 
CCCTCAGAGA ACTCTGAAGG CAAACAAACA CAAGCCAAGT TGCAGGCTCT GCTGGAAAAG 
ACCCAACTGA AGTTGCTTCC CGAGGAAGAG AGTCGATTGC TACAACTGGA AGAGAAGTGG 
CGCCGCCTGC GAGTGAACAA GGGCGCCGTG GAAACACGTT CACCGCTTGA ACTGATACGG 
TCCCACTTTG CCCAAGCCCT GGCGAAGTGC AGCGGCCCCC ACGTCCAACC GGAAGCCAGG 
CCCGCCTCCT CGTCCTCCCT GTCGGACTAC AGTGGGGAGG AGAGTGCAGA GGAGGAGCCC 
CAGCGGCCAC CTGCCAGCGC CCGGCGTGTC CACGAGCAGG TTCCAGCTCC CTGGGAGCAG 
GAAGTGCGCT CCTGGGACAA GGTCCAGGTG TTCCTCGATG GCCTGCTGCG CAACAGCGAC 
ATCCAGCGCA GTAACAACAA CCCAACGCCC GCGAGGAAGG CCAACAACTC GACCAACACA 
CTCGTCATCC GCCACGGCAC AAGCGGTCGC AAGTGTCGAG TCAAGGCGGC GCAGAGCCGA 
AAAGTGGAAG CGCCCAAGGA CAGGTCTCAG GAAAACGTGA GCCTGAAGGT GCTTTTCGAA 
AACAACCCGG AACTGCGGCA GGAAGGTCAG ACTTGTTCGG TGCTTGAGGT CTTGGAGCCC 
TCGAGCAGCG ATGCAGAAGA AGACCTCAAC GTGGGACTGC GGCGCGGAAG CGGGGACAGC 
TCGTCGAGCT CCTTCCGCTC TAATGAGAAT GCGGTGCTTC ACGTGGCGGA GCAGCGGTGT 
CCCAAGAAAC CCGTAGCTGC GAGCAATGGC GAGCGCAAGG GCAAGGGCGC CATCCTTCTG 
ACCAACATCG CAGAGCTGCC CGATTGCTCA AGCAGGTACA AGGTCAGCTT CAAGATCCAG 
CAGTCGCAGG AATGCCCTCA CCTCGGCTCG GTAGAGACGC AGCCCAAGGA TGAAGCAGAC 
GCGGCAAATG ACAAGAAGGG CAGAAACGCG GAGCGGCAGA TGAGTTTCGC AGAGCCCGAT 
TTGATTCGGG GGACCAACGT GGCAGAACGT TGCGCCCCGG AGCGCAGCAC CGCGGAGGCC 
TCTTCGGCGG GCGAGTGCCC CTCGGAGCTG TCCTTCGGAG ACTCGGCCAG CCAGGACGTC 
ACGGGGACGG AGGACGTGGA GGAGCAGCAG CATGCGGCGC AGGGTCTCTC ATCCCCAGAG 
TGCTCCGTGC CAGCAGGCGA AACCTCTTCC GGGGCGGGAA GTGCCAAAAA GCGCAGCGGA 
TTTCTGCTCA AGTTCTCGCT AAGGGGCCGC TGGCCGTCCA AACAGAAGCA GCAGCGGAAG 
AGCGAGGAGA TCAGCGCGGA GGAGTTTCGA GAAACATACA CGCGGAGTGC GGGGGCGGAG 
CCCTTCAGCC GGGACCCCCT TCCGGAGCAA AGCACCACGG CCAAAGCGAG CGAGCTGTTT 
GCGCTCTCAC CGCCCCCAAT CCCCACGCTT CCAAGGCGGG GTCCTCCCCC TGTGCCCCCG 
TGCGAGGAGG AGGTGCCGCC CGAGTCCCCC GAGCCCTCCC TGGGGAGCAA GTGCTCCCCG 
ACCGAGGACG AGGCCCTACT CTACCTGCAT GGGGGGTCGC CAGAGCCCAG CATCTTGAGG 
CCAGATTCCA TCGTCTCTAA GACCACCGGA GGCTCCCTGT CCCGCCCGGC GTCCTCGGCG 
TCCCAGACGG AGGACGGGAG GCGGGGTGCC AGGGAAGGGG CGGGCCGGGG GAATGTGGCC 
GTGGTGGGCT TCTCCCTGCC CAAGACGCTA TCCTCCACTG AGGTGTTGCT GCTGCACACA 
CCGGAGTCCT CCGTGGCAGG GCGGCAGTCG CCTGCCCCCA GCGAGGTCTC CAAGACGGAG 
AGCGCCCTGT CACCGCCCAG CGACGTGTCC AAGACGGAGA GTGCGAGGCA GGCACCACCC 
CTGGGAAAAA TCGAGGAGAT TGTGGCGGCC GCGGGGGCCG CCGCTGCTGC ATCAAGCGCG 
GAGAAGAGGG TGGACCCGTC GGCCGGTGCC CTCAGGGCCC GGCCTTCCGC GGGTGTGGAG 
GCCCCCAAGC AAGAGGGCCA TCCCTGCATG ACCCCCGTGA CCATTCTGCA GCAGGACGTC 
CCGCCGCATC AGGCAAAGGC CAAAAAGATG ACATTGGCTG CTAGGAAAAA CAAGAAAAGT 
TCAAAGCCCT GGTACGAGGT TTCGGACGAG GAGGACATGC TGTCTCCTGA CAAGTACCAG 
ACAGTCAGGG CTCGCAGCAG CTCCGAAGAC GAGACAGACC TGGCTGTTGT GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029846648.1
CDS	567..4523
Translation	MRAQLQGPPTAPRQRPLLHQVPWGVSQNDLEAVEDILSATSLVASPVRPGVLSSRRSRAGAAGHGASCGGCPITDTTRAPRAAALTAQPAAAGPRAAAKPRRPGAQQAPAWADPSRGTGSSSGDVLSGAAAMRHPDDGGAVEEFVLDDRDLLRLCEVPLQRSRTAAAGPRRPHLRTVSIIDTLSALARGAADRDVLPPPPRDPWARGAAPPPPPYARRTVQAGGGSHWRRSRRQERDWSRQCASDSEVKGGGDACSVLSLRSQPPRRSSAQERPAAARVPRVPNWICAIFRLAKKGDLEALSQSLRDMERELVWNLSDAQGNSLWHVCAARNHLRCFQWLCQGHPQHVLADENHSSLTPVGTAVKHGNLEIVQWLVSKSWALEQINPPEGNRTLLHLAAKYGQERVVRWLAEYMQNNNLNINRKDNDGNTPVHLAAKHGHISVIKTLVLLDADVTAQNEQGLRPHGVAVQSGQVACADHLLTTEVCLCLSSEQVLIEGNFQSLQVENAEMKNGFRELLVLTKRLLRRQEEMLHYVQVVCSGNMNSDKAESDSADSLPWNSPSENSEGKQTQAKLQALLEKTQLKLLPEEESRLLQLEEKWRRLRVNKGAVETRSPLELIRSHFAQALAKCSGPHVQPEARPASSSSLSDYSGEESAEEEPQRPPASARRVHEQVPAPWEQEVRSWDKVQVFLDGLLRNSDIQRSNNNPTPARKANNSTNTLVIRHGTSGRKCRVKAAQSRKVEAPKDRSQENVSLKVLFENNPELRQEGQTCSVLEVLEPSSSDAEEDLNVGLRRGSGDSSSSSFRSNENAVLHVAEQRCPKKPVAASNGERKGKGAILLTNIAELPDCSSRYKVSFKIQQSQECPHLGSVETQPKDEADAANDKKGRNAERQMSFAEPDLIRGTNVAERCAPERSTAEASSAGECPSELSFGDSASQDVTGTEDVEEQQHAAQGLSSPECSVPAGETSSGAGSAKKRSGFLLKFSLRGRWPSKQKQQRKSEEISAEEFRETYTRSAGAEPFSRDPLPEQSTTAKASELFALSPPPIPTLPRRGPPPVPPCEEEVPPESPEPSLGSKCSPTEDEALLYLHGGSPEPSILRPDSIVSKTTGGSLSRPASSASQTEDGRRGAREGAGRGNVAVVGFSLPKTLSSTEVLLLHTPESSVAGRQSPAPSEVSKTESALSPPSDVSKTESARQAPPLGKIEEIVAAAGAAAAASSAEKRVDPSAGALRARPSAGVEAPKQEGHPCMTPVTILQQDVPPHQAKAKKMTLAARKNKKSSKPWYEVSDEEDMLSPDKYQTVRARSSSEDETDLAVVA

Target ORF information:

RefSeq Version	XM_029990788.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8031915 (LOC8031915), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029990788.1

ORF Insert Sequence:

ATGCGGGCCC AGCTGCAGGG GCCGCCCACG GCGCCCAGGC AGCGCCCGCT CCTGCACCAG 
GTCCCGTGGG GAGTGTCCCA GAACGACCTT GAAGCCGTCG AGGACATTCT GTCGGCCACG 
TCACTCGTCG CATCGCCTGT GCGTCCGGGC GTGCTGTCGT CTCGGCGGAG TCGTGCCGGA 
GCTGCGGGCC ATGGGGCATC CTGTGGGGGC TGCCCGATCA CGGACACGAC ACGAGCGCCC 
AGGGCCGCCG CTCTTACCGC ACAGCCTGCA GCGGCGGGTC CCCGAGCGGC GGCCAAGCCT 
CGGCGCCCCG GCGCCCAGCA AGCCCCGGCT TGGGCGGACC CCAGCCGCGG CACCGGCAGC 
AGCAGCGGCG ACGTCTTGAG CGGCGCCGCC GCCATGCGGC ACCCGGACGA CGGCGGGGCC 
GTCGAGGAGT TCGTCCTCGA CGACCGAGAC CTGCTGCGCC TCTGCGAGGT ACCGCTGCAG 
CGCAGCAGGA CCGCCGCGGC GGGTCCCCGG CGGCCGCACC TGCGCACGGT CTCCATCATC 
GACACCCTGA GCGCGCTGGC CCGGGGCGCC GCCGACCGGG ACGTGTTGCC GCCGCCGCCA 
CGGGACCCCT GGGCGCGCGG TGCGGCGCCG CCGCCGCCGC CGTACGCGCG GCGCACGGTC 
CAGGCCGGGG GCGGGTCGCA CTGGCGGCGG TCGCGGCGGC AGGAGCGCGA CTGGTCGCGG 
CAGTGCGCCT CGGACAGCGA GGTGAAGGGC GGTGGCGACG CGTGCTCGGT GCTGTCGCTG 
CGCAGCCAGC CGCCCCGGAG GAGCAGCGCC CAGGAGCGGC CGGCCGCTGC CAGGGTGCCC 
AGAGTGCCCA ACTGGATATG CGCCATCTTC AGGCTCGCCA AGAAGGGGGA CCTCGAGGCT 
CTGAGCCAGA GCCTGCGAGA CATGGAGCGG GAGCTGGTTT GGAACCTGTC AGATGCCCAG 
GGCAACAGCC TGTGGCACGT GTGCGCGGCC CGGAACCACC TGCGCTGCTT CCAGTGGCTC 
TGCCAGGGAC ACCCACAGCA CGTCCTGGCT GACGAGAACC ACAGCAGCCT CACGCCCGTC 
GGAACTGCCG TCAAGCATGG GAACCTGGAG ATAGTCCAAT GGCTCGTCAG CAAGTCCTGG 
GCCCTGGAAC AAATTAACCC TCCAGAGGGG AACAGGACCT TGCTTCATCT AGCTGCAAAG 
TATGGTCAGG AGAGAGTGGT GCGTTGGCTG GCAGAGTATA TGCAGAACAA CAACCTGAAC 
ATCAACCGGA AGGACAATGA CGGAAACACT CCGGTGCATC TGGCTGCGAA ACATGGACAC 
ATCTCCGTCA TCAAGACCCT GGTGCTGTTG GATGCGGACG TGACGGCTCA GAACGAGCAA 
GGCCTGCGGC CGCATGGGGT GGCCGTGCAG AGTGGCCAGG TGGCCTGTGC GGACCACCTG 
CTCACCACCG AGGTCTGCCT CTGTCTCTCC TCTGAGCAGG TTCTCATCGA GGGAAACTTC 
CAGAGCTTGC AAGTTGAAAA TGCTGAGATG AAGAATGGCT TCAGAGAGCT GCTGGTGCTT 
ACCAAGAGGC TGTTGAGGAG ACAGGAGGAG ATGCTACACT ATGTGCAAGT GGTCTGTTCG 
GGAAACATGA ACTCGGACAA GGCGGAGTCG GACAGTGCAG ACTCACTTCC GTGGAACTCC 
CCCTCAGAGA ACTCTGAAGG CAAACAAACA CAAGCCAAGT TGCAGGCTCT GCTGGAAAAG 
ACCCAACTGA AGTTGCTTCC CGAGGAAGAG AGTCGATTGC TACAACTGGA AGAGAAGTGG 
CGCCGCCTGC GAGTGAACAA GGGCGCCGTG GAAACACGTT CACCGCTTGA ACTGATACGG 
TCCCACTTTG CCCAAGCCCT GGCGAAGTGC AGCGGCCCCC ACGTCCAACC GGAAGCCAGG 
CCCGCCTCCT CGTCCTCCCT GTCGGACTAC AGTGGGGAGG AGAGTGCAGA GGAGGAGCCC 
CAGCGGCCAC CTGCCAGCGC CCGGCGTGTC CACGAGCAGG TTCCAGCTCC CTGGGAGCAG 
GAAGTGCGCT CCTGGGACAA GGTCCAGGTG TTCCTCGATG GCCTGCTGCG CAACAGCGAC 
ATCCAGCGCA GTAACAACAA CCCAACGCCC GCGAGGAAGG CCAACAACTC GACCAACACA 
CTCGTCATCC GCCACGGCAC AAGCGGTCGC AAGTGTCGAG TCAAGGCGGC GCAGAGCCGA 
AAAGTGGAAG CGCCCAAGGA CAGGTCTCAG GAAAACGTGA GCCTGAAGGT GCTTTTCGAA 
AACAACCCGG AACTGCGGCA GGAAGGTCAG ACTTGTTCGG TGCTTGAGGT CTTGGAGCCC 
TCGAGCAGCG ATGCAGAAGA AGACCTCAAC GTGGGACTGC GGCGCGGAAG CGGGGACAGC 
TCGTCGAGCT CCTTCCGCTC TAATGAGAAT GCGGTGCTTC ACGTGGCGGA GCAGCGGTGT 
CCCAAGAAAC CCGTAGCTGC GAGCAATGGC GAGCGCAAGG GCAAGGGCGC CATCCTTCTG 
ACCAACATCG CAGAGCTGCC CGATTGCTCA AGCAGGTACA AGGTCAGCTT CAAGATCCAG 
CAGTCGCAGG AATGCCCTCA CCTCGGCTCG GTAGAGACGC AGCCCAAGGA TGAAGCAGAC 
GCGGCAAATG ACAAGAAGGG CAGAAACGCG GAGCGGCAGA TGAGTTTCGC AGAGCCCGAT 
TTGATTCGGG GGACCAACGT GGCAGAACGT TGCGCCCCGG AGCGCAGCAC CGCGGAGGCC 
TCTTCGGCGG GCGAGTGCCC CTCGGAGCTG TCCTTCGGAG ACTCGGCCAG CCAGGACGTC 
ACGGGGACGG AGGACGTGGA GGAGCAGCAG CATGCGGCGC AGGGTCTCTC ATCCCCAGAG 
TGCTCCGTGC CAGCAGGCGA AACCTCTTCC GGGGCGGGAA GTGCCAAAAA GCGCAGCGGA 
TTTCTGCTCA AGTTCTCGCT AAGGGGCCGC TGGCCGTCCA AACAGAAGCA GCAGCGGAAG 
AGCGAGGAGA TCAGCGCGGA GGAGTTTCGA GAAACATACA CGCGGAGTGC GGGGGCGGAG 
CCCTTCAGCC GGGACCCCCT TCCGGAGCAA AGCACCACGG CCAAAGCGAG CGAGCTGTTT 
GCGCTCTCAC CGCCCCCAAT CCCCACGCTT CCAAGGCGGG GTCCTCCCCC TGTGCCCCCG 
TGCGAGGAGG AGGTGCCGCC CGAGTCCCCC GAGCCCTCCC TGGGGAGCAA GTGCTCCCCG 
ACCGAGGACG AGGCCCTACT CTACCTGCAT GGGGGGTCGC CAGAGCCCAG CATCTTGAGG 
CCAGATTCCA TCGTCTCTAA GACCACCGGA GGCTCCCTGT CCCGCCCGGC GTCCTCGGCG 
TCCCAGACGG AGGACGGGAG GCGGGGTGCC AGGGAAGGGG CGGGCCGGGG GAATGTGGCC 
GTGGTGGGCT TCTCCCTGCC CAAGACGCTA TCCTCCACTG AGGTGTTGCT GCTGCACACA 
CCGGAGTCCT CCGTGGCAGG GCGGCAGTCG CCTGCCCCCA GCGAGGTCTC CAAGACGGAG 
AGCGCCCTGT CACCGCCCAG CGACGTGTCC AAGACGGAGA GTGCGAGGCA GGCACCACCC 
CTGGGAAAAA TCGAGGAGAT TGTGGCGGCC GCGGGGGCCG CCGCTGCTGC ATCAAGCGCG 
GAGAAGAGGG TGGACCCGTC GGCCGGTGCC CTCAGGGCCC GGCCTTCCGC GGGTGTGGAG 
GCCCCCAAGC AAGAGGGCCA TCCCTGCATG ACCCCCGTGA CCATTCTGCA GCAGGACGTC 
CCGCCGCATC AGGCAAAGGC CAAAAAGATG ACATTGGCTG CTAGGAAAAA CAAGAAAAGT 
TCAAAGCCCT GGTACGAGGT TTCGGACGAG GAGGACATGC TGTCTCCTGA CAAGTACCAG 
ACAGTCAGGG CTCGCAGCAG CTCCGAAGAC GAGACAGACC TGGCTGTTGT GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8031915 ( XM_029990793.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029846653.1 Ixodes scapularis uncharacterized LOC8031915 (LOC8031915), transcript variant X2, mRNA.

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CloneID	OIa16173
Clone ID Related Accession (Same CDS sequence)	XM_029990793.1
Accession Version	XM_029990793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8031915 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021917938.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCCC AGCTGCAGGG GCCGCCCACG GCGCCCAGGC AGCGCCCGCT CCTGCACCAG 
GTCCCGTGGG GAGTGTCCCA GAACGACCTT GAAGCCGTCG AGGACATTCT GTCGGCCACG 
TCACTCGTCG CATCGCCTGT GCGTCCGGGC GTGCTGTCGT CTCGGCGGAG TCGTGCCGGA 
GCTGCGGGCC ATGGGGCATC CTGTGGGGGC TGCCCGATCA CGGACACGAC ACGAGCGCCC 
AGGGCCGCCG CTCTTACCGC ACAGAGCCAG AGCCTGCGAG ACATGGAGCG GGAGCTGGTT 
TGGAACCTGT CAGATGCCCA GGGCAACAGC CTGTGGCACG TGTGCGCGGC CCGGAACCAC 
CTGCGCTGCT TCCAGTGGCT CTGCCAGGGA CACCCACAGC ACGTCCTGGC TGACGAGAAC 
CACAGCAGCC TCACGCCCGT CGGAACTGCC GTCAAGCATG GGAACCTGGA GATAGTCCAA 
TGGCTCGTCA GCAAGTCCTG GGCCCTGGAA CAAATTAACC CTCCAGAGGG GAACAGGACC 
TTGCTTCATC TAGCTGCAAA GTATGGTCAG GAGAGAGTGG TGCGTTGGCT GGCAGAGTAT 
ATGCAGAACA ACAACCTGAA CATCAACCGG AAGGACAATG ACGGAAACAC TCCGGTGCAT 
CTGGCTGCGA AACATGGACA CATCTCCGTC ATCAAGACCC TGGTGCTGTT GGATGCGGAC 
GTGACGGCTC AGAACGAGCA AGGCCTGCGG CCGCATGGGG TGGCCGTGCA GAGTGGCCAG 
GTGGCCTGTG CGGACCACCT GCTCACCACC GAGGTCTGCC TCTGTCTCTC CTCTGAGCAG 
GTTCTCATCG AGGGAAACTT CCAGAGCTTG CAAGTTGAAA ATGCTGAGAT GAAGAATGGC 
TTCAGAGAGC TGCTGGTGCT TACCAAGAGG CTGTTGAGGA GACAGGAGGA GATGCTACAC 
TATGTGCAAG TGGTCTGTTC GGGAAACATG AACTCGGACA AGGCGGAGTC GGACAGTGCA 
GACTCACTTC CGTGGAACTC CCCCTCAGAG AACTCTGAAG GCAAACAAAC ACAAGCCAAG 
TTGCAGGCTC TGCTGGAAAA GACCCAACTG AAGTTGCTTC CCGAGGAAGA GAGTCGATTG 
CTACAACTGG AAGAGAAGTG GCGCCGCCTG CGAGTGAACA AGGGCGCCGT GGAAACACGT 
TCACCGCTTG AACTGATACG GTCCCACTTT GCCCAAGCCC TGGCGAAGTG CAGCGGCCCC 
CACGTCCAAC CGGAAGCCAG GCCCGCCTCC TCGTCCTCCC TGTCGGACTA CAGTGGGGAG 
GAGAGTGCAG AGGAGGAGCC CCAGCGGCCA CCTGCCAGCG CCCGGCGTGT CCACGAGCAG 
GTTCCAGCTC CCTGGGAGCA GGAAGTGCGC TCCTGGGACA AGGTCCAGGT GTTCCTCGAT 
GGCCTGCTGC GCAACAGCGA CATCCAGCGC AGTAACAACA ACCCAACGCC CGCGAGGAAG 
GCCAACAACT CGACCAACAC ACTCGTCATC CGCCACGGCA CAAGCGGTCG CAAGTGTCGA 
GTCAAGGCGG CGCAGAGCCG AAAAGTGGAA GCGCCCAAGG ACAGGTCTCA GGAAAACGTG 
AGCCTGAAGG TGCTTTTCGA AAACAACCCG GAACTGCGGC AGGAAGGTCA GACTTGTTCG 
GTGCTTGAGG TCTTGGAGCC CTCGAGCAGC GATGCAGAAG AAGACCTCAA CGTGGGACTG 
CGGCGCGGAA GCGGGGACAG CTCGTCGAGC TCCTTCCGCT CTAATGAGAA TGCGGTGCTT 
CACGTGGCGG AGCAGCGGTG TCCCAAGAAA CCCGTAGCTG CGAGCAATGG CGAGCGCAAG 
GGCAAGGGCG CCATCCTTCT GACCAACATC GCAGAGCTGC CCGATTGCTC AAGCAGGTAC 
AAGGTCAGCT TCAAGATCCA GCAGTCGCAG GAATGCCCTC ACCTCGGCTC GGTAGAGACG 
CAGCCCAAGG ATGAAGCAGA CGCGGCAAAT GACAAGAAGG GCAGAAACGC GGAGCGGCAG 
ATGAGTTTCG CAGAGCCCGA TTTGATTCGG GGGACCAACG TGGCAGAACG TTGCGCCCCG 
GAGCGCAGCA CCGCGGAGGC CTCTTCGGCG GGCGAGTGCC CCTCGGAGCT GTCCTTCGGA 
GACTCGGCCA GCCAGGACGT CACGGGGACG GAGGACGTGG AGGAGCAGCA GCATGCGGCG 
CAGGGTCTCT CATCCCCAGA GTGCTCCGTG CCAGCAGGCG AAACCTCTTC CGGGGCGGGA 
AGTGCCAAAA AGCGCAGCGG ATTTCTGCTC AAGTTCTCGC TAAGGGGCCG CTGGCCGTCC 
AAACAGAAGC AGCAGCGGAA GAGCGAGGAG ATCAGCGCGG AGGAGTTTCG AGAAACATAC 
ACGCGGAGTG CGGGGGCGGA GCCCTTCAGC CGGGACCCCC TTCCGGAGCA AAGCACCACG 
GCCAAAGCGA GCGAGCTGTT TGCGCTCTCA CCGCCCCCAA TCCCCACGCT TCCAAGGCGG 
GGTCCTCCCC CTGTGCCCCC GTGCGAGGAG GAGGTGCCGC CCGAGTCCCC CGAGCCCTCC 
CTGGGGAGCA AGTGCTCCCC GACCGAGGAC GAGGCCCTAC TCTACCTGCA TGGGGGGTCG 
CCAGAGCCCA GCATCTTGAG GCCAGATTCC ATCGTCTCTA AGACCACCGG AGGCTCCCTG 
TCCCGCCCGG CGTCCTCGGC GTCCCAGACG GAGGACGGGA GGCGGGGTGC CAGGGAAGGG 
GCGGGCCGGG GGAATGTGGC CGTGGTGGGC TTCTCCCTGC CCAAGACGCT ATCCTCCACT 
GAGGTGTTGC TGCTGCACAC ACCGGAGTCC TCCGTGGCAG GGCGGCAGTC GCCTGCCCCC 
AGCGAGGTCT CCAAGACGGA GAGCGCCCTG TCACCGCCCA GCGACGTGTC CAAGACGGAG 
AGTGCGAGGC AGGCACCACC CCTGGGAAAA ATCGAGGAGA TTGTGGCGGC CGCGGGGGCC 
GCCGCTGCTG CATCAAGCGC GGAGAAGAGG GTGGACCCGT CGGCCGGTGC CCTCAGGGCC 
CGGCCTTCCG CGGGTGTGGA GGCCCCCAAG CAAGAGGGCC ATCCCTGCAT GACCCCCGTG 
ACCATTCTGC AGCAGGACGT CCCGCCGCAT CAGGCAAAGG CCAAAAAGAT GACATTGGCT 
GCTAGGAAAA ACAAGAAAAG TTCAAAGCCC TGGTACGAGG TTTCGGACGA GGAGGACATG 
CTGTCTCCTG ACAAGTACCA GACAGTCAGG GCTCGCAGCA GCTCCGAAGA CGAGACAGAC 
CTGGCTGTTG TGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029846653.1
CDS	567..3884
Translation	MRAQLQGPPTAPRQRPLLHQVPWGVSQNDLEAVEDILSATSLVASPVRPGVLSSRRSRAGAAGHGASCGGCPITDTTRAPRAAALTAQSQSLRDMERELVWNLSDAQGNSLWHVCAARNHLRCFQWLCQGHPQHVLADENHSSLTPVGTAVKHGNLEIVQWLVSKSWALEQINPPEGNRTLLHLAAKYGQERVVRWLAEYMQNNNLNINRKDNDGNTPVHLAAKHGHISVIKTLVLLDADVTAQNEQGLRPHGVAVQSGQVACADHLLTTEVCLCLSSEQVLIEGNFQSLQVENAEMKNGFRELLVLTKRLLRRQEEMLHYVQVVCSGNMNSDKAESDSADSLPWNSPSENSEGKQTQAKLQALLEKTQLKLLPEEESRLLQLEEKWRRLRVNKGAVETRSPLELIRSHFAQALAKCSGPHVQPEARPASSSSLSDYSGEESAEEEPQRPPASARRVHEQVPAPWEQEVRSWDKVQVFLDGLLRNSDIQRSNNNPTPARKANNSTNTLVIRHGTSGRKCRVKAAQSRKVEAPKDRSQENVSLKVLFENNPELRQEGQTCSVLEVLEPSSSDAEEDLNVGLRRGSGDSSSSSFRSNENAVLHVAEQRCPKKPVAASNGERKGKGAILLTNIAELPDCSSRYKVSFKIQQSQECPHLGSVETQPKDEADAANDKKGRNAERQMSFAEPDLIRGTNVAERCAPERSTAEASSAGECPSELSFGDSASQDVTGTEDVEEQQHAAQGLSSPECSVPAGETSSGAGSAKKRSGFLLKFSLRGRWPSKQKQQRKSEEISAEEFRETYTRSAGAEPFSRDPLPEQSTTAKASELFALSPPPIPTLPRRGPPPVPPCEEEVPPESPEPSLGSKCSPTEDEALLYLHGGSPEPSILRPDSIVSKTTGGSLSRPASSASQTEDGRRGAREGAGRGNVAVVGFSLPKTLSSTEVLLLHTPESSVAGRQSPAPSEVSKTESALSPPSDVSKTESARQAPPLGKIEEIVAAAGAAAAASSAEKRVDPSAGALRARPSAGVEAPKQEGHPCMTPVTILQQDVPPHQAKAKKMTLAARKNKKSSKPWYEVSDEEDMLSPDKYQTVRARSSSEDETDLAVVA

Target ORF information:

RefSeq Version	XM_029990793.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8031915 (LOC8031915), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029990793.1

ORF Insert Sequence:

ATGCGGGCCC AGCTGCAGGG GCCGCCCACG GCGCCCAGGC AGCGCCCGCT CCTGCACCAG 
GTCCCGTGGG GAGTGTCCCA GAACGACCTT GAAGCCGTCG AGGACATTCT GTCGGCCACG 
TCACTCGTCG CATCGCCTGT GCGTCCGGGC GTGCTGTCGT CTCGGCGGAG TCGTGCCGGA 
GCTGCGGGCC ATGGGGCATC CTGTGGGGGC TGCCCGATCA CGGACACGAC ACGAGCGCCC 
AGGGCCGCCG CTCTTACCGC ACAGAGCCAG AGCCTGCGAG ACATGGAGCG GGAGCTGGTT 
TGGAACCTGT CAGATGCCCA GGGCAACAGC CTGTGGCACG TGTGCGCGGC CCGGAACCAC 
CTGCGCTGCT TCCAGTGGCT CTGCCAGGGA CACCCACAGC ACGTCCTGGC TGACGAGAAC 
CACAGCAGCC TCACGCCCGT CGGAACTGCC GTCAAGCATG GGAACCTGGA GATAGTCCAA 
TGGCTCGTCA GCAAGTCCTG GGCCCTGGAA CAAATTAACC CTCCAGAGGG GAACAGGACC 
TTGCTTCATC TAGCTGCAAA GTATGGTCAG GAGAGAGTGG TGCGTTGGCT GGCAGAGTAT 
ATGCAGAACA ACAACCTGAA CATCAACCGG AAGGACAATG ACGGAAACAC TCCGGTGCAT 
CTGGCTGCGA AACATGGACA CATCTCCGTC ATCAAGACCC TGGTGCTGTT GGATGCGGAC 
GTGACGGCTC AGAACGAGCA AGGCCTGCGG CCGCATGGGG TGGCCGTGCA GAGTGGCCAG 
GTGGCCTGTG CGGACCACCT GCTCACCACC GAGGTCTGCC TCTGTCTCTC CTCTGAGCAG 
GTTCTCATCG AGGGAAACTT CCAGAGCTTG CAAGTTGAAA ATGCTGAGAT GAAGAATGGC 
TTCAGAGAGC TGCTGGTGCT TACCAAGAGG CTGTTGAGGA GACAGGAGGA GATGCTACAC 
TATGTGCAAG TGGTCTGTTC GGGAAACATG AACTCGGACA AGGCGGAGTC GGACAGTGCA 
GACTCACTTC CGTGGAACTC CCCCTCAGAG AACTCTGAAG GCAAACAAAC ACAAGCCAAG 
TTGCAGGCTC TGCTGGAAAA GACCCAACTG AAGTTGCTTC CCGAGGAAGA GAGTCGATTG 
CTACAACTGG AAGAGAAGTG GCGCCGCCTG CGAGTGAACA AGGGCGCCGT GGAAACACGT 
TCACCGCTTG AACTGATACG GTCCCACTTT GCCCAAGCCC TGGCGAAGTG CAGCGGCCCC 
CACGTCCAAC CGGAAGCCAG GCCCGCCTCC TCGTCCTCCC TGTCGGACTA CAGTGGGGAG 
GAGAGTGCAG AGGAGGAGCC CCAGCGGCCA CCTGCCAGCG CCCGGCGTGT CCACGAGCAG 
GTTCCAGCTC CCTGGGAGCA GGAAGTGCGC TCCTGGGACA AGGTCCAGGT GTTCCTCGAT 
GGCCTGCTGC GCAACAGCGA CATCCAGCGC AGTAACAACA ACCCAACGCC CGCGAGGAAG 
GCCAACAACT CGACCAACAC ACTCGTCATC CGCCACGGCA CAAGCGGTCG CAAGTGTCGA 
GTCAAGGCGG CGCAGAGCCG AAAAGTGGAA GCGCCCAAGG ACAGGTCTCA GGAAAACGTG 
AGCCTGAAGG TGCTTTTCGA AAACAACCCG GAACTGCGGC AGGAAGGTCA GACTTGTTCG 
GTGCTTGAGG TCTTGGAGCC CTCGAGCAGC GATGCAGAAG AAGACCTCAA CGTGGGACTG 
CGGCGCGGAA GCGGGGACAG CTCGTCGAGC TCCTTCCGCT CTAATGAGAA TGCGGTGCTT 
CACGTGGCGG AGCAGCGGTG TCCCAAGAAA CCCGTAGCTG CGAGCAATGG CGAGCGCAAG 
GGCAAGGGCG CCATCCTTCT GACCAACATC GCAGAGCTGC CCGATTGCTC AAGCAGGTAC 
AAGGTCAGCT TCAAGATCCA GCAGTCGCAG GAATGCCCTC ACCTCGGCTC GGTAGAGACG 
CAGCCCAAGG ATGAAGCAGA CGCGGCAAAT GACAAGAAGG GCAGAAACGC GGAGCGGCAG 
ATGAGTTTCG CAGAGCCCGA TTTGATTCGG GGGACCAACG TGGCAGAACG TTGCGCCCCG 
GAGCGCAGCA CCGCGGAGGC CTCTTCGGCG GGCGAGTGCC CCTCGGAGCT GTCCTTCGGA 
GACTCGGCCA GCCAGGACGT CACGGGGACG GAGGACGTGG AGGAGCAGCA GCATGCGGCG 
CAGGGTCTCT CATCCCCAGA GTGCTCCGTG CCAGCAGGCG AAACCTCTTC CGGGGCGGGA 
AGTGCCAAAA AGCGCAGCGG ATTTCTGCTC AAGTTCTCGC TAAGGGGCCG CTGGCCGTCC 
AAACAGAAGC AGCAGCGGAA GAGCGAGGAG ATCAGCGCGG AGGAGTTTCG AGAAACATAC 
ACGCGGAGTG CGGGGGCGGA GCCCTTCAGC CGGGACCCCC TTCCGGAGCA AAGCACCACG 
GCCAAAGCGA GCGAGCTGTT TGCGCTCTCA CCGCCCCCAA TCCCCACGCT TCCAAGGCGG 
GGTCCTCCCC CTGTGCCCCC GTGCGAGGAG GAGGTGCCGC CCGAGTCCCC CGAGCCCTCC 
CTGGGGAGCA AGTGCTCCCC GACCGAGGAC GAGGCCCTAC TCTACCTGCA TGGGGGGTCG 
CCAGAGCCCA GCATCTTGAG GCCAGATTCC ATCGTCTCTA AGACCACCGG AGGCTCCCTG 
TCCCGCCCGG CGTCCTCGGC GTCCCAGACG GAGGACGGGA GGCGGGGTGC CAGGGAAGGG 
GCGGGCCGGG GGAATGTGGC CGTGGTGGGC TTCTCCCTGC CCAAGACGCT ATCCTCCACT 
GAGGTGTTGC TGCTGCACAC ACCGGAGTCC TCCGTGGCAG GGCGGCAGTC GCCTGCCCCC 
AGCGAGGTCT CCAAGACGGA GAGCGCCCTG TCACCGCCCA GCGACGTGTC CAAGACGGAG 
AGTGCGAGGC AGGCACCACC CCTGGGAAAA ATCGAGGAGA TTGTGGCGGC CGCGGGGGCC 
GCCGCTGCTG CATCAAGCGC GGAGAAGAGG GTGGACCCGT CGGCCGGTGC CCTCAGGGCC 
CGGCCTTCCG CGGGTGTGGA GGCCCCCAAG CAAGAGGGCC ATCCCTGCAT GACCCCCGTG 
ACCATTCTGC AGCAGGACGT CCCGCCGCAT CAGGCAAAGG CCAAAAAGAT GACATTGGCT 
GCTAGGAAAA ACAAGAAAAG TTCAAAGCCC TGGTACGAGG TTTCGGACGA GGAGGACATG 
CTGTCTCCTG ACAAGTACCA GACAGTCAGG GCTCGCAGCA GCTCCGAAGA CGAGACAGAC 
CTGGCTGTTG TGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8031915 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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