IscW_ISCW007213 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	IscW_ISCW007213
Entrez Gene ID	8030922
Full Name	LIN-12 protein, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029989613.1	XP_029845473.1	neurogenic locus Notch protein
XM_002403510.1	XP_002403554.1	LIN-12 protein, putative

Pathways from BioSystems
Gene Ontology

The following IscW_ISCW007213 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IscW_ISCW007213 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIa04438	XM_002403510.1 Latest version!	Ixodes scapularis LIN-12 protein, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OIa31286	XM_029989613.1 Latest version!	Ixodes scapularis neurogenic locus Notch protein (LOC8030922), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IscW_ISCW007213 ( XM_002403510.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002403554.1 Ixodes scapularis LIN-12 protein, putative, mRNA.

Price & Availability↑

CloneID	OIa04438
Clone ID Related Accession (Same CDS sequence)	XM_002403510.1
Accession Version	XM_002403510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	LIN-12 protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002675814). COMPLETENESS: incomplete on the 5' end.

ATGGCGAGCG TGATCGACAA CCAGATCTAT GCTCCGCTCG GATTATACAG CAATTGTCCC 
CCGCTCTTCG TGGGCGAGTA CTGCCAGCAC ATGAACCCCT GCCAGGCCGT GTCGGGCAAG 
CGGTGTCAGA ACGGCGGCAC GTGCAACGTC GAGATGAGCG TGACCAGCGG GCCGAGCTTC 
TCGTGCACCT GTCCCGTGGG CTACTCGGCC AGCATGTGCG AGATCGCCGT GCCCAGCGCC 
TGCGACGACC GGCCCTGCCG CAACGGCGGC ACCTGTTCCC TCGTCACCCT CGACAACTAC 
ACGTGCGCTT GCGCCACGGG CTTCAGGGGC AAGCACTGCG ACAAGAAGGA CCACTGCGCG 
TCTCAGCCGT GCCTCAACTA CGGCAGTTGC CTGTCCAGGG CGGACTCCTA CGCCTGCGTG 
TGTGCCCAGG GGTACAGCGG CCCCACGTGC GCCGTCGACG TGGACGAGTG CAAGGCAAAG 
CCCTGCGAGC AAGGCACTTG CATCAACACC CAGGGATCCT TCAGCTGTGT CTGCGAGGCG 
GGCTTCACGG GCCAGCTGTG CGAGTCCCGG TACATCCCGT GCGAGCCGAG CCCCTGCCTG 
AACGGCGGGA CCTGCAGACC CATCGACTCG CTCAACTACC AGTGCTCGTG TCCTCCTGGT 
TTCACGGGCA CCCACTGCGA GGCGAACGTA GACGACTGTC CGGGCAACCT GTGCCAGAAT 
GGCGCCACCT GCCTGGACGG AGTCAACTCG TACACCTGCC ACTGTCCGCC CACGTACACC 
GGGCCGTACT GCAGCCGCGA CGTGGACGAG TGCTCGTTCC GACCCACGGT GTGCAAGAAC 
GGCGCCACGT GCACCAACAC CGTGGGCGGA TATTCGTGCA TCTGCGTCAA CGGATGGACG 
GGCACCGACT GCTCCGAGAA CATCGACGAC TGCGCTGTGG CGGCCTGCTT CAACGGCGCC 
ACGTGCCACG ACCGCGTCGG GTCCTTCTAC TGCCAGTGCG CTCCTGGGAA GACAGGGCTG 
CTGTGTCACC TGGACGACGC GTGCGCCAGC AACCCGTGCC ACGAGGGCGC CATCTGCGAC 
ACAAGCCCCA TCGACGGCAC CTACCTGTGC TCGTGCCCCA ACGGCTTCCA GGGCGTGGAC 
TGCACCGAGG ACGTGGACGA AGAGGCGACG CAGGGGGAGG AGGAGGAGGG CTACAAGGGC 
ATTCACTGCG AAGTGGATAT CGACGAGTGC CTCCCCAACC CATGCCTCAA CGGAGGTATC 
TGCAACGATC TCATCAATGG CTTCAAGTGC CTGTGTCCTG TGGGTTTCTC GGGCAAGAAG 
TGTGAAGCCA ACGAAGACGA TTGCTCTTCC TTCCCGTGCC GCAATGGAGG CTCCTGCCAT 
GATGGCATTG CCAGCTATAC ATGCCACTGT CCACCTGGCT TCACGGGTGC CACGTGTGAG 
ACGAACATCA ACGACTGCCA GTCTTCGCCG TGCCACCATG GCGTCTGCCA CGACGGCATC 
AACTCCTTCA GCTGCAACTG CCACGCGGGC TACACGGGGC TGCTCTGCCA GACCCAGATC 
AACGAGTGCC TAAGCAGTCC CTGCCGCCAC GGCGGGACAT GCGAGGACCT CGTCAACGGT 
TACCAGTGTC GCTGCCGTCC GGGCACCTCC GGGGTCGACT GCGAGTACAA CGTCAACGAG 
TGCTTCTCCA ACCCCTGCCG GCACGGAGCA AAGTGCATCG ACGGGATAGA CTCCTACACT 
TGCGACTGCC TTGCCGGATT CACTGGCATC CACTGCGAGA CCAACATCAA CGAGTGCGCC 
AGCAACCCCT GTTCTAATGG TGGCGTCTGC ACCGACCTGG TCAACGGATT TAAGTGCGAC 
TGCCCCCGCG GCTACTTTGA TGCCCGCTGC CTCTCCGACG TCAACGAGTG CGCCAGCAAC 
CCGTGCCAGA ACGGAGGCAC CTGCGAAGAC GAGGTCAACC GCTTCGTCTG CCACTGCCCT 
CCCGGTTACG GGGGCCACCG CTGCGAGCAA GACATAGACG AGTGCCAGTC CAATCCGTGC 
CAGCATGGTG GAAGCTGCCA CGACACCCTC AATGCTTACT CGTGCACCTG CATAGACGGC 
TACTCCGGGC GGAATTGCGA GACGAACCTT GACGACTGCA GTCCTAATCC GTGCCTCAAT 
GGCGGAAGTT GCATTGACCT GGTCGGGACG TTCCGCTGCG TGTGTGAGGT GCCCTTTTCC 
GGTCCCACGT GCGACGTCGA GTTGGACCCC TGCTCTCCCA ACAAGTGTCG CAACGGTGCC 
CAGTGTTCGC CGTCCTCGAA TTACCTCGAC TTTGCCTGCT CCTGCAAGTT GGGCTTCACG 
GGGAGGTTGT GCGACGAGGA CATCGACGAG TGTGCGGTGT CGTCGCCGTG CCGCAATGAG 
GCCACGTGCG TCAACGTAAA CGGATCGTAC GAGTGCCTCT GCACCCGCGG CTACGAGGGT 
CGGGACTGCC TCACCAACAC CGACGACTGC GCTTCGTACC CCTGCCAGAA TGGAGGAACG 
TGTCTGGATG GCATTGGAGA GTACACTTGT CTGTGCGTTG ATGGCTTTGG CGGAGAGCAC 
TGCGCCGACG ACATGAACGA GTGTGCCAGC AACCCCTGCC AGAATGGAGC CACCTGCAAT 
GACTATGTGA ACAGCTATGC CTGTGCCTGC CTCCCGGGCT TCAGCGGAAC CAACTGCCAG 
ACCAACGATG AGGACTGCAC CCCAAGTTCC TGCATGAACG GCGGCACATG CGTGGACGGA 
GTGAACAACT ACACGTGCCA GTGCAGTCCG GGCTACACGG GCTCCAACTG CCAGTACCAC 
ATCAACGAGT GTGACTCCCA GCCCTGTGCC CATGGGGCCA CCTGTGTCCA CCACATTGGC 
TACCACACCT GCCACTGCCC CTTCGGGTAC ATGGGACCAC GCTGCGAGAC CTTTGTGGAC 
TGGTGTGCCA CCAACCCATG CCTCAATGGA GCCAGCTGTA AGCAGTCCAA CAACACGTAT 
CGCTGCACCT GCCAACCTGG GTGGACAGGC CTGCTCTGTG ACGTGTCCAT GGTGTCATGT 
GAAGATGCTG CCCTACAGAA AGGCACCAAA GTGGCCGACC TCTGCAAGCA CGGCGGAGTG 
TGCGAAGACT TCGGCAACAG CCACCACTGT CTCTGCCCCG AAGGCTACGA GGGCAGCTAC 
TGCCAGCGCG AAGTCAACGA GTGCCTGAGC AACCCGTGCC AGAACGGAGC CACCTGCCAC 
GACCTCCTGG GCCAGTATGC CTGTGACTGC CCCGAGGGCT TCCAGGGGCT CAACTGCGAG 
TACGACGTCA ACGACTGCGA CCCTAGCCCC TGCCGCAATG GCGGCACCTG CCACGATCTC 
GTCAACAAGT TTGTCTGCTC CTGCCCCCAC GGCACCCTCG GCACCCTCTG CGAGATCGAC 
GTCAACGAGT GCTTTGAGAG CGCCTGCCAC CACGGAGGCA CCTGCCTCGA CCGCGTCGGC 
AGGTACGAGT GCCAATGCCC GCCTGGATAC GTGGGCTCCC GCTGCGAAGG CGATGTCAAC 
GAGTGCCTCT CGAGCCCCTG TGACCCGCTA GGGACCCTAG ACTGCGTGCA GCTCGTCAAT 
GACTACCGCT GCGACTGCCG CCCGGGTCAT GCGGGGCGCC GGTGCGAGCT CAAGGTCGAC 
CCTTGCTCCA CGGACCCGTG CCTCAACGGC GGCGTCTGCC ACCCGGGGCC ACGGGGTCCG 
ACCTGCGTCT GCCAGGAGGG CTTTTGGGGC GAAACCTGCG CCAACCGCAG CTGCGGAGAC 
GCCAGCCCGT GCCGCAATGG GGGACAGTGC CGCGGCCAGT CCTGCGTCTG TCCCCGCGGA 
ACTTCCGGAA GCTTCTGCGA GCGGCTGGAG GAGCTTGGTG CCGGTGGTGC CTGCCCGCTG 
GGGTGCCGCA ACGGGGGAGC GTGCCGCAAG GGTGCAAGCG GGAGGTTCGA GTGCGAGTGC 
CTAGTTGGAT GGAAGGGGCT GCGGTGCGAA GAATACGACT CCAGCTTCCG CGGTGCCTTG 
GCGCCTTCAG TGGAGGGTGT TTATGACCTC TTCCTCAAGT CTCTGGAAGA AGAGAAGAAG 
CTGTGTGCCG CTCGGAGGTG TGAGAAGAAG GCGGGAAACC GCTACTGTGA TGAAGAGTGC 
AACACGTACG CTTGCAATTT CGATGGTGGT GACTGCTCTC TTGGAATTAA TCCTTGGATG 
AACTGCACTG CTGCCATCAA GTGCTGGGAC GTGTTCAGAG ACGGAAAGTG CAATGTCGAG 
TGCAACAATA TCGACTGCCT CTTTGATGGC TTTGATTGCC AGAAAGACCT CCAACCTTGC 
AATGAGCACT ATGACACCTA CTGCATGAGC AACTATGGTA ATGGCTACTG CGACTACGGC 
TGCAACAACG AGGAGTGCAA CTGGGACGGG CTCGACTGTG AAATGGATCC CCCTTACCTG 
GCGGAGGGTT CCATTGTCAT CATGATCTCC ATCGAGCCAG AGGTGTTCCG GAACAACACG 
GTGCCCTTCT TGCGTGACAT TGGCCACGCT CTTCGCACCA ATGTGCTCTT CAAGCGGGAC 
CCCACAGGCA GCCCCATGAT CTACCCTTGG CGGGCCACCG ATGGACAGAA ACCCAACCAC 
GCGGCACTGC CCGTACCGCG CACAGGGACG CTGGTGCACC TGGAGATTGA CAACCGGCGC 
TGCCTCAAGT CTCAGGGCAG CGAGTGCTTC CAGTCTGCGG CCAAGGCGGC AGAGTTCCTG 
GCAGCAGCCC AGGCCCGGCA CTCCCTCGAG ACCCCGTTCC ACATCCAGGA GGTGCAGGGG 
GCTGGCCGGG AGCAGGGAGG CGACGGGGCG GGCGACGGGG GCCGGGCCAG TGCCCTGCAC 
GTGGTCATCG GCCTCATCGG CGTGCTGCTG GCGGGCCTGC TGGTGGGCGT GCTGTTCACC 
ACGCAGAAGA AGCGGGCCCG GGGCATCACC TGGTTCCCCG AGGGCTTTCT CCGTACCGGC 
AGCGGTCAAA GAAGGCACTC GAGGCGCAGG GGCCCCGATG GACAGGAGAT GAGAGCGGCT 
CACGCCAATG GCGAGGGCCC TCCCGAAGCC TGGAGCGACG ACGAAGGAGA GCGTCCACCG 
GCCAAACGTG CACGCGGTTC CCCCGACTCG AGCTGTGGAG ACACGGTGGT GTCCGACTGC 
GAGGAGGAGA GGGACCCCCG ACCCTGGACC CAGCAGCACC TGGAGGCTGC GGACGTGCAT 
CACCCAGAGC TGCTGCTGGC CCTGACCCCA CCCCAGGGCA ATGATCTCGA CCAGCACAAC 
GTGGACGTCA GGGGCCCTGG TGGCCTCACC CCGCTAATGC TGGCCTCTTT TCGCGGGAAT 
GGCCTGGACA CTGGCGAAGA TGTGGGCGAG GATGACGACG GCTCTGCCAG CATGATCCAG 
GATCTGCTCA TGCAGGGAGC GCAGCTTGCC AGTACCACTG AAAAGACTGG CGAGACTCCC 
CTGCACTTGG CCGCACGCTA CGCTCGTGCG GATGCCGCCA AGCGCCTCCT CGACGCCGGT 
GCAGAGGCCA ATGCGCAGGA CAGTTCGGGC AGAACGCCCC TGCATGCCGC CATTGCTGCC 
GACGCCGTGG GAGTGTTCCA GATCTTGCTA CGCAATCGCG CCACCAACCT CAACGCCAAG 
ATGAACAATG GGATGACGCC GCTCATCCTC TCCATCCGCC TGGCCATGGA GGGAATGGCC 
GAGGACCTCA TCAACGCCGA GGCTGATGTC AATGCGGCTG ACTGCGACGC CCGCACGCCC 
CTTCACTGGG CCGCCGCCGT CAATGACGTC ACCTCCGTGC GCATCCTGCT CGCGCATGGC 
GCAAACCGCG ACGCGCAAGA CATTCACGAA GAGACTCCGC TGTTCTTAGC AGCCCGTGAG 
GGCAGCCTCA ATGCGGTGAG GGCGCTGCTC GACCACGCTG CCAACCGAGA CATCACCAAC 
CACATGGACC GGCGACCGCG TGACGTGGCT CGTGACCGCA TGCACCTTGA CATCGTCGAC 
CTGCTCGACA AGTATGTCCC GTCGTCCCAG ATCCCCGGGC TGGGCAGCCC CCCTATGCTG 
CTCTCCAATC CGACCGTCAT TGGTTCCACC AAGTCGGCCA AGGGCAAGCG GCGCCAGCAG 
CCCAAGAACA ATGGCAACGC GAGCATCGCG CTGCAGCACC TTGACGCGTC TGCGACGTCA 
CCCGGCGCGG AGGTCAACCA TGTCAAGCGG CGGCCCTCCA TCAAGAAGCG AAGGGAACCA 
CCCATGCCGT TGCCAGAGGT GCCCCACCCG CTCGACTCTC CGCACGAATC TCCGGGTGGG 
CCGCTCTTCC TCCAGCAGGG CGGAGTGGCC ACGCTGACCA AGCAGCCACC GTGCTACGAG 
GACTGCGTGG GGCCCGTGTA CACGGCCGTG TACGACCCAC AGGCCTACGC CAACGCCCCG 
ATGGCCCAGC AGCAACAGCA CCCGCGACAG AACTCGGTCC CGTCCATGGG TGGATACACG 
ATGGCCTCTT CGCCTGCCAA GCAGCGCCCC TCGCTGCCCA CGTCGCCCAC CCACATGGCG 
GCCATGAGGG CCGCACACCA GCACAAGCTG CAGCAGGGTG CCCCCACGTA TGACTACCCC 
GTCCAGCCAG CCTACTACCA CTACCCTACG CCCCCGTCCC AGCACAGCCA TGGCGGCGGG 
CCCGAGGCCA CGCCCCAGCA CTACCTCCAC CCGGCGGAGA CTTACCTGAC GCCCTCGCCG 
GAGTCTCCAG GACAGTGGTC CAGCTCCTCG CCGCACTCGG CGCAGTCCGA CTGGTCGGAA 
GGGATCTCCA GCCCCCTTGG GCAGCAGGCG CTTGGGGGAT GCGAGCTCAA GCAGCAGCAG 
CAGCAGCAGA CCGTCCAGCC GGCTGGACTG ACCCACCCGG ATCCGGTGTT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002403554.1
CDS	1..6897
Translation	MASVIDNQIYAPLGLYSNCPPLFVGEYCQHMNPCQAVSGKRCQNGGTCNVEMSVTSGPSFSCTCPVGYSASMCEIAVPSACDDRPCRNGGTCSLVTLDNYTCACATGFRGKHCDKKDHCASQPCLNYGSCLSRADSYACVCAQGYSGPTCAVDVDECKAKPCEQGTCINTQGSFSCVCEAGFTGQLCESRYIPCEPSPCLNGGTCRPIDSLNYQCSCPPGFTGTHCEANVDDCPGNLCQNGATCLDGVNSYTCHCPPTYTGPYCSRDVDECSFRPTVCKNGATCTNTVGGYSCICVNGWTGTDCSENIDDCAVAACFNGATCHDRVGSFYCQCAPGKTGLLCHLDDACASNPCHEGAICDTSPIDGTYLCSCPNGFQGVDCTEDVDEEATQGEEEEGYKGIHCEVDIDECLPNPCLNGGICNDLINGFKCLCPVGFSGKKCEANEDDCSSFPCRNGGSCHDGIASYTCHCPPGFTGATCETNINDCQSSPCHHGVCHDGINSFSCNCHAGYTGLLCQTQINECLSSPCRHGGTCEDLVNGYQCRCRPGTSGVDCEYNVNECFSNPCRHGAKCIDGIDSYTCDCLAGFTGIHCETNINECASNPCSNGGVCTDLVNGFKCDCPRGYFDARCLSDVNECASNPCQNGGTCEDEVNRFVCHCPPGYGGHRCEQDIDECQSNPCQHGGSCHDTLNAYSCTCIDGYSGRNCETNLDDCSPNPCLNGGSCIDLVGTFRCVCEVPFSGPTCDVELDPCSPNKCRNGAQCSPSSNYLDFACSCKLGFTGRLCDEDIDECAVSSPCRNEATCVNVNGSYECLCTRGYEGRDCLTNTDDCASYPCQNGGTCLDGIGEYTCLCVDGFGGEHCADDMNECASNPCQNGATCNDYVNSYACACLPGFSGTNCQTNDEDCTPSSCMNGGTCVDGVNNYTCQCSPGYTGSNCQYHINECDSQPCAHGATCVHHIGYHTCHCPFGYMGPRCETFVDWCATNPCLNGASCKQSNNTYRCTCQPGWTGLLCDVSMVSCEDAALQKGTKVADLCKHGGVCEDFGNSHHCLCPEGYEGSYCQREVNECLSNPCQNGATCHDLLGQYACDCPEGFQGLNCEYDVNDCDPSPCRNGGTCHDLVNKFVCSCPHGTLGTLCEIDVNECFESACHHGGTCLDRVGRYECQCPPGYVGSRCEGDVNECLSSPCDPLGTLDCVQLVNDYRCDCRPGHAGRRCELKVDPCSTDPCLNGGVCHPGPRGPTCVCQEGFWGETCANRSCGDASPCRNGGQCRGQSCVCPRGTSGSFCERLEELGAGGACPLGCRNGGACRKGASGRFECECLVGWKGLRCEEYDSSFRGALAPSVEGVYDLFLKSLEEEKKLCAARRCEKKAGNRYCDEECNTYACNFDGGDCSLGINPWMNCTAAIKCWDVFRDGKCNVECNNIDCLFDGFDCQKDLQPCNEHYDTYCMSNYGNGYCDYGCNNEECNWDGLDCEMDPPYLAEGSIVIMISIEPEVFRNNTVPFLRDIGHALRTNVLFKRDPTGSPMIYPWRATDGQKPNHAALPVPRTGTLVHLEIDNRRCLKSQGSECFQSAAKAAEFLAAAQARHSLETPFHIQEVQGAGREQGGDGAGDGGRASALHVVIGLIGVLLAGLLVGVLFTTQKKRARGITWFPEGFLRTGSGQRRHSRRRGPDGQEMRAAHANGEGPPEAWSDDEGERPPAKRARGSPDSSCGDTVVSDCEEERDPRPWTQQHLEAADVHHPELLLALTPPQGNDLDQHNVDVRGPGGLTPLMLASFRGNGLDTGEDVGEDDDGSASMIQDLLMQGAQLASTTEKTGETPLHLAARYARADAAKRLLDAGAEANAQDSSGRTPLHAAIAADAVGVFQILLRNRATNLNAKMNNGMTPLILSIRLAMEGMAEDLINAEADVNAADCDARTPLHWAAAVNDVTSVRILLAHGANRDAQDIHEETPLFLAAREGSLNAVRALLDHAANRDITNHMDRRPRDVARDRMHLDIVDLLDKYVPSSQIPGLGSPPMLLSNPTVIGSTKSAKGKRRQQPKNNGNASIALQHLDASATSPGAEVNHVKRRPSIKKRREPPMPLPEVPHPLDSPHESPGGPLFLQQGGVATLTKQPPCYEDCVGPVYTAVYDPQAYANAPMAQQQQHPRQNSVPSMGGYTMASSPAKQRPSLPTSPTHMAAMRAAHQHKLQQGAPTYDYPVQPAYYHYPTPPSQHSHGGGPEATPQHYLHPAETYLTPSPESPGQWSSSSPHSAQSDWSEGISSPLGQQALGGCELKQQQQQQTVQPAGLTHPDPVFI

Target ORF information:

RefSeq Version	XM_002403510.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis LIN-12 protein, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002403510.1

ORF Insert Sequence:

ATGGCGAGCG TGATCGACAA CCAGATCTAT GCTCCGCTCG GATTATACAG CAATTGTCCC 
CCGCTCTTCG TGGGCGAGTA CTGCCAGCAC ATGAACCCCT GCCAGGCCGT GTCGGGCAAG 
CGGTGTCAGA ACGGCGGCAC GTGCAACGTC GAGATGAGCG TGACCAGCGG GCCGAGCTTC 
TCGTGCACCT GTCCCGTGGG CTACTCGGCC AGCATGTGCG AGATCGCCGT GCCCAGCGCC 
TGCGACGACC GGCCCTGCCG CAACGGCGGC ACCTGTTCCC TCGTCACCCT CGACAACTAC 
ACGTGCGCTT GCGCCACGGG CTTCAGGGGC AAGCACTGCG ACAAGAAGGA CCACTGCGCG 
TCTCAGCCGT GCCTCAACTA CGGCAGTTGC CTGTCCAGGG CGGACTCCTA CGCCTGCGTG 
TGTGCCCAGG GGTACAGCGG CCCCACGTGC GCCGTCGACG TGGACGAGTG CAAGGCAAAG 
CCCTGCGAGC AAGGCACTTG CATCAACACC CAGGGATCCT TCAGCTGTGT CTGCGAGGCG 
GGCTTCACGG GCCAGCTGTG CGAGTCCCGG TACATCCCGT GCGAGCCGAG CCCCTGCCTG 
AACGGCGGGA CCTGCAGACC CATCGACTCG CTCAACTACC AGTGCTCGTG TCCTCCTGGT 
TTCACGGGCA CCCACTGCGA GGCGAACGTA GACGACTGTC CGGGCAACCT GTGCCAGAAT 
GGCGCCACCT GCCTGGACGG AGTCAACTCG TACACCTGCC ACTGTCCGCC CACGTACACC 
GGGCCGTACT GCAGCCGCGA CGTGGACGAG TGCTCGTTCC GACCCACGGT GTGCAAGAAC 
GGCGCCACGT GCACCAACAC CGTGGGCGGA TATTCGTGCA TCTGCGTCAA CGGATGGACG 
GGCACCGACT GCTCCGAGAA CATCGACGAC TGCGCTGTGG CGGCCTGCTT CAACGGCGCC 
ACGTGCCACG ACCGCGTCGG GTCCTTCTAC TGCCAGTGCG CTCCTGGGAA GACAGGGCTG 
CTGTGTCACC TGGACGACGC GTGCGCCAGC AACCCGTGCC ACGAGGGCGC CATCTGCGAC 
ACAAGCCCCA TCGACGGCAC CTACCTGTGC TCGTGCCCCA ACGGCTTCCA GGGCGTGGAC 
TGCACCGAGG ACGTGGACGA AGAGGCGACG CAGGGGGAGG AGGAGGAGGG CTACAAGGGC 
ATTCACTGCG AAGTGGATAT CGACGAGTGC CTCCCCAACC CATGCCTCAA CGGAGGTATC 
TGCAACGATC TCATCAATGG CTTCAAGTGC CTGTGTCCTG TGGGTTTCTC GGGCAAGAAG 
TGTGAAGCCA ACGAAGACGA TTGCTCTTCC TTCCCGTGCC GCAATGGAGG CTCCTGCCAT 
GATGGCATTG CCAGCTATAC ATGCCACTGT CCACCTGGCT TCACGGGTGC CACGTGTGAG 
ACGAACATCA ACGACTGCCA GTCTTCGCCG TGCCACCATG GCGTCTGCCA CGACGGCATC 
AACTCCTTCA GCTGCAACTG CCACGCGGGC TACACGGGGC TGCTCTGCCA GACCCAGATC 
AACGAGTGCC TAAGCAGTCC CTGCCGCCAC GGCGGGACAT GCGAGGACCT CGTCAACGGT 
TACCAGTGTC GCTGCCGTCC GGGCACCTCC GGGGTCGACT GCGAGTACAA CGTCAACGAG 
TGCTTCTCCA ACCCCTGCCG GCACGGAGCA AAGTGCATCG ACGGGATAGA CTCCTACACT 
TGCGACTGCC TTGCCGGATT CACTGGCATC CACTGCGAGA CCAACATCAA CGAGTGCGCC 
AGCAACCCCT GTTCTAATGG TGGCGTCTGC ACCGACCTGG TCAACGGATT TAAGTGCGAC 
TGCCCCCGCG GCTACTTTGA TGCCCGCTGC CTCTCCGACG TCAACGAGTG CGCCAGCAAC 
CCGTGCCAGA ACGGAGGCAC CTGCGAAGAC GAGGTCAACC GCTTCGTCTG CCACTGCCCT 
CCCGGTTACG GGGGCCACCG CTGCGAGCAA GACATAGACG AGTGCCAGTC CAATCCGTGC 
CAGCATGGTG GAAGCTGCCA CGACACCCTC AATGCTTACT CGTGCACCTG CATAGACGGC 
TACTCCGGGC GGAATTGCGA GACGAACCTT GACGACTGCA GTCCTAATCC GTGCCTCAAT 
GGCGGAAGTT GCATTGACCT GGTCGGGACG TTCCGCTGCG TGTGTGAGGT GCCCTTTTCC 
GGTCCCACGT GCGACGTCGA GTTGGACCCC TGCTCTCCCA ACAAGTGTCG CAACGGTGCC 
CAGTGTTCGC CGTCCTCGAA TTACCTCGAC TTTGCCTGCT CCTGCAAGTT GGGCTTCACG 
GGGAGGTTGT GCGACGAGGA CATCGACGAG TGTGCGGTGT CGTCGCCGTG CCGCAATGAG 
GCCACGTGCG TCAACGTAAA CGGATCGTAC GAGTGCCTCT GCACCCGCGG CTACGAGGGT 
CGGGACTGCC TCACCAACAC CGACGACTGC GCTTCGTACC CCTGCCAGAA TGGAGGAACG 
TGTCTGGATG GCATTGGAGA GTACACTTGT CTGTGCGTTG ATGGCTTTGG CGGAGAGCAC 
TGCGCCGACG ACATGAACGA GTGTGCCAGC AACCCCTGCC AGAATGGAGC CACCTGCAAT 
GACTATGTGA ACAGCTATGC CTGTGCCTGC CTCCCGGGCT TCAGCGGAAC CAACTGCCAG 
ACCAACGATG AGGACTGCAC CCCAAGTTCC TGCATGAACG GCGGCACATG CGTGGACGGA 
GTGAACAACT ACACGTGCCA GTGCAGTCCG GGCTACACGG GCTCCAACTG CCAGTACCAC 
ATCAACGAGT GTGACTCCCA GCCCTGTGCC CATGGGGCCA CCTGTGTCCA CCACATTGGC 
TACCACACCT GCCACTGCCC CTTCGGGTAC ATGGGACCAC GCTGCGAGAC CTTTGTGGAC 
TGGTGTGCCA CCAACCCATG CCTCAATGGA GCCAGCTGTA AGCAGTCCAA CAACACGTAT 
CGCTGCACCT GCCAACCTGG GTGGACAGGC CTGCTCTGTG ACGTGTCCAT GGTGTCATGT 
GAAGATGCTG CCCTACAGAA AGGCACCAAA GTGGCCGACC TCTGCAAGCA CGGCGGAGTG 
TGCGAAGACT TCGGCAACAG CCACCACTGT CTCTGCCCCG AAGGCTACGA GGGCAGCTAC 
TGCCAGCGCG AAGTCAACGA GTGCCTGAGC AACCCGTGCC AGAACGGAGC CACCTGCCAC 
GACCTCCTGG GCCAGTATGC CTGTGACTGC CCCGAGGGCT TCCAGGGGCT CAACTGCGAG 
TACGACGTCA ACGACTGCGA CCCTAGCCCC TGCCGCAATG GCGGCACCTG CCACGATCTC 
GTCAACAAGT TTGTCTGCTC CTGCCCCCAC GGCACCCTCG GCACCCTCTG CGAGATCGAC 
GTCAACGAGT GCTTTGAGAG CGCCTGCCAC CACGGAGGCA CCTGCCTCGA CCGCGTCGGC 
AGGTACGAGT GCCAATGCCC GCCTGGATAC GTGGGCTCCC GCTGCGAAGG CGATGTCAAC 
GAGTGCCTCT CGAGCCCCTG TGACCCGCTA GGGACCCTAG ACTGCGTGCA GCTCGTCAAT 
GACTACCGCT GCGACTGCCG CCCGGGTCAT GCGGGGCGCC GGTGCGAGCT CAAGGTCGAC 
CCTTGCTCCA CGGACCCGTG CCTCAACGGC GGCGTCTGCC ACCCGGGGCC ACGGGGTCCG 
ACCTGCGTCT GCCAGGAGGG CTTTTGGGGC GAAACCTGCG CCAACCGCAG CTGCGGAGAC 
GCCAGCCCGT GCCGCAATGG GGGACAGTGC CGCGGCCAGT CCTGCGTCTG TCCCCGCGGA 
ACTTCCGGAA GCTTCTGCGA GCGGCTGGAG GAGCTTGGTG CCGGTGGTGC CTGCCCGCTG 
GGGTGCCGCA ACGGGGGAGC GTGCCGCAAG GGTGCAAGCG GGAGGTTCGA GTGCGAGTGC 
CTAGTTGGAT GGAAGGGGCT GCGGTGCGAA GAATACGACT CCAGCTTCCG CGGTGCCTTG 
GCGCCTTCAG TGGAGGGTGT TTATGACCTC TTCCTCAAGT CTCTGGAAGA AGAGAAGAAG 
CTGTGTGCCG CTCGGAGGTG TGAGAAGAAG GCGGGAAACC GCTACTGTGA TGAAGAGTGC 
AACACGTACG CTTGCAATTT CGATGGTGGT GACTGCTCTC TTGGAATTAA TCCTTGGATG 
AACTGCACTG CTGCCATCAA GTGCTGGGAC GTGTTCAGAG ACGGAAAGTG CAATGTCGAG 
TGCAACAATA TCGACTGCCT CTTTGATGGC TTTGATTGCC AGAAAGACCT CCAACCTTGC 
AATGAGCACT ATGACACCTA CTGCATGAGC AACTATGGTA ATGGCTACTG CGACTACGGC 
TGCAACAACG AGGAGTGCAA CTGGGACGGG CTCGACTGTG AAATGGATCC CCCTTACCTG 
GCGGAGGGTT CCATTGTCAT CATGATCTCC ATCGAGCCAG AGGTGTTCCG GAACAACACG 
GTGCCCTTCT TGCGTGACAT TGGCCACGCT CTTCGCACCA ATGTGCTCTT CAAGCGGGAC 
CCCACAGGCA GCCCCATGAT CTACCCTTGG CGGGCCACCG ATGGACAGAA ACCCAACCAC 
GCGGCACTGC CCGTACCGCG CACAGGGACG CTGGTGCACC TGGAGATTGA CAACCGGCGC 
TGCCTCAAGT CTCAGGGCAG CGAGTGCTTC CAGTCTGCGG CCAAGGCGGC AGAGTTCCTG 
GCAGCAGCCC AGGCCCGGCA CTCCCTCGAG ACCCCGTTCC ACATCCAGGA GGTGCAGGGG 
GCTGGCCGGG AGCAGGGAGG CGACGGGGCG GGCGACGGGG GCCGGGCCAG TGCCCTGCAC 
GTGGTCATCG GCCTCATCGG CGTGCTGCTG GCGGGCCTGC TGGTGGGCGT GCTGTTCACC 
ACGCAGAAGA AGCGGGCCCG GGGCATCACC TGGTTCCCCG AGGGCTTTCT CCGTACCGGC 
AGCGGTCAAA GAAGGCACTC GAGGCGCAGG GGCCCCGATG GACAGGAGAT GAGAGCGGCT 
CACGCCAATG GCGAGGGCCC TCCCGAAGCC TGGAGCGACG ACGAAGGAGA GCGTCCACCG 
GCCAAACGTG CACGCGGTTC CCCCGACTCG AGCTGTGGAG ACACGGTGGT GTCCGACTGC 
GAGGAGGAGA GGGACCCCCG ACCCTGGACC CAGCAGCACC TGGAGGCTGC GGACGTGCAT 
CACCCAGAGC TGCTGCTGGC CCTGACCCCA CCCCAGGGCA ATGATCTCGA CCAGCACAAC 
GTGGACGTCA GGGGCCCTGG TGGCCTCACC CCGCTAATGC TGGCCTCTTT TCGCGGGAAT 
GGCCTGGACA CTGGCGAAGA TGTGGGCGAG GATGACGACG GCTCTGCCAG CATGATCCAG 
GATCTGCTCA TGCAGGGAGC GCAGCTTGCC AGTACCACTG AAAAGACTGG CGAGACTCCC 
CTGCACTTGG CCGCACGCTA CGCTCGTGCG GATGCCGCCA AGCGCCTCCT CGACGCCGGT 
GCAGAGGCCA ATGCGCAGGA CAGTTCGGGC AGAACGCCCC TGCATGCCGC CATTGCTGCC 
GACGCCGTGG GAGTGTTCCA GATCTTGCTA CGCAATCGCG CCACCAACCT CAACGCCAAG 
ATGAACAATG GGATGACGCC GCTCATCCTC TCCATCCGCC TGGCCATGGA GGGAATGGCC 
GAGGACCTCA TCAACGCCGA GGCTGATGTC AATGCGGCTG ACTGCGACGC CCGCACGCCC 
CTTCACTGGG CCGCCGCCGT CAATGACGTC ACCTCCGTGC GCATCCTGCT CGCGCATGGC 
GCAAACCGCG ACGCGCAAGA CATTCACGAA GAGACTCCGC TGTTCTTAGC AGCCCGTGAG 
GGCAGCCTCA ATGCGGTGAG GGCGCTGCTC GACCACGCTG CCAACCGAGA CATCACCAAC 
CACATGGACC GGCGACCGCG TGACGTGGCT CGTGACCGCA TGCACCTTGA CATCGTCGAC 
CTGCTCGACA AGTATGTCCC GTCGTCCCAG ATCCCCGGGC TGGGCAGCCC CCCTATGCTG 
CTCTCCAATC CGACCGTCAT TGGTTCCACC AAGTCGGCCA AGGGCAAGCG GCGCCAGCAG 
CCCAAGAACA ATGGCAACGC GAGCATCGCG CTGCAGCACC TTGACGCGTC TGCGACGTCA 
CCCGGCGCGG AGGTCAACCA TGTCAAGCGG CGGCCCTCCA TCAAGAAGCG AAGGGAACCA 
CCCATGCCGT TGCCAGAGGT GCCCCACCCG CTCGACTCTC CGCACGAATC TCCGGGTGGG 
CCGCTCTTCC TCCAGCAGGG CGGAGTGGCC ACGCTGACCA AGCAGCCACC GTGCTACGAG 
GACTGCGTGG GGCCCGTGTA CACGGCCGTG TACGACCCAC AGGCCTACGC CAACGCCCCG 
ATGGCCCAGC AGCAACAGCA CCCGCGACAG AACTCGGTCC CGTCCATGGG TGGATACACG 
ATGGCCTCTT CGCCTGCCAA GCAGCGCCCC TCGCTGCCCA CGTCGCCCAC CCACATGGCG 
GCCATGAGGG CCGCACACCA GCACAAGCTG CAGCAGGGTG CCCCCACGTA TGACTACCCC 
GTCCAGCCAG CCTACTACCA CTACCCTACG CCCCCGTCCC AGCACAGCCA TGGCGGCGGG 
CCCGAGGCCA CGCCCCAGCA CTACCTCCAC CCGGCGGAGA CTTACCTGAC GCCCTCGCCG 
GAGTCTCCAG GACAGTGGTC CAGCTCCTCG CCGCACTCGG CGCAGTCCGA CTGGTCGGAA 
GGGATCTCCA GCCCCCTTGG GCAGCAGGCG CTTGGGGGAT GCGAGCTCAA GCAGCAGCAG 
CAGCAGCAGA CCGTCCAGCC GGCTGGACTG ACCCACCCGG ATCCGGTGTT CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW007213 ( XM_029989613.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029845473.1 Ixodes scapularis neurogenic locus Notch protein (LOC8030922), mRNA.

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CloneID	OIa31286
Clone ID Related Accession (Same CDS sequence)	XM_029989613.1
Accession Version	XM_029989613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	neurogenic locus Notch protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921402.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCT GCCAGGCCGT GTCGGGCAAG CGGTGTCAGA ACGGCGGCAC GTGCAACGTC 
GAGATGAGCG TGACCAGCGG GCCGAGCTTC TCGTGCACCT GTCCCGTGGG CTACTCGGCC 
AGCATGTGCG AGATCGCCGT GCCCAGCGCC TGCGACGACC GGCCCTGCCG CAACGGCGGC 
ACCTGTTCCC TCGTCACCCT CGACAACTAC ACGTGCGCTT GCGCCACGGG CTTCAGGGGC 
AAGCACTGCG ACAAGAAGGA CCATTGCGCG TCTCAGCCGT GCCTCAACTA CGGCAGCTGC 
CTGTCCAGGG CGGACTCCTA CGCCTGCGTG TGTGCCCAGG GATACAGCGG CCCCACGTGC 
GCCGTCGACG TGGACGAGTG CAAGGCAAAG CCCTGCGAGC AAGGCACTTG CATCAACACC 
CAGGGATCCT TCAGCTGTGT CTGCGAGGCG GGCTTCACGG GCCAGCTGTG CGAGTCCCGG 
TACATCCCGT GCGAGCCGAG CCCCTGCCTG AACGGCGGGA CCTGCAGACC CATCGACTCG 
CTCAACTACC AGTGCTCGTG TCCTCCTGGT TTCACGGGCA CCCACTGCGA GGCGAACGTA 
GACGACTGTC CGGGCAACCT GTGCCAGAAT GGCGCCACCT GCCTGGACGG AGTCAACTCG 
TACACCTGCC ACTGTCCGCC CACGTACACC GGGCCGTACT GCAGCCGCGA CGTGGACGAG 
TGCTCGTTCC GGCCAACGGT GTGCAAGAAC GGCGCCACGT GCACCAACAC CGTGGGCGGA 
TACTCGTGCA TCTGCGTCAA CGGATGGACG GGCACCGACT GCTCCGAGAA CATCGACGAC 
TGCGCTGTGG CGGCCTGCTT CAACGGCGCC ACGTGCCACG ACCGCGTCGG GTCCTTCTAC 
TGCCAGTGCG CTCCTGGGAA GACAGGGCTG CTGTGTCACC TGGACGACGC GTGCGCGAGC 
AACCCGTGCC ACGAGGGCGC CATCTGCGAC ACAAGCCCCA TCGACGGCAC CTACCTGTGC 
TCGTGCCCCA ACGGCTTCCA GGGCGTGGAC TGCACCGAGG ACGTGGACGA GTGCGCCCAG 
GGGGAGCCCT GCGAGCACGG CGGTATCTGC GTCAACACGC CGGGCTCGTT CCGCTGCAAC 
TGCACCCGGG GCTTCGCCGG GCCGCGCTGC GAGGTGAACA TCAACGAGTG CGACTCCAAC 
CCCTGCCACA ACGAGGGAAC CTGCCTGGAC GAGCGCGGAG GCTTCCGATG CGTCTGCATG 
CCCGGCTACA AGGGCATTCA CTGCGAAGTG GATATCGACG AGTGCCTCCC CAACCCATGC 
CTCAACGGAG GTATCTGCAA CGATCTCATC AATGGCTTCA AGTGCCTGTG TCCTGTGGGT 
TTCTCGGGCA AGAAGTGTGA AGCCAACGAA GACGATTGCT CTTCCTTCCC GTGCCGCAAT 
GGAGGCTCCT GCCATGATGG CATTGCCAGC TATACTTGCC ACTGTCCACC TGGCTTCACG 
GGTGCCACGT GTGAGACGAA CATCAACGAC TGCCAGTCTT CGCCGTGCCA CCATGGCGTC 
TGCCACGACG GCATCAACTC CTTCAGCTGC AACTGCCACG CGGGCTACAC GGGGCTGCTC 
TGCCAGACCC AGATCAACGA GTGCCTAAGC AGTCCCTGCC GCCACGGCGG GACATGCGAG 
GACCTCGTCA ACGGCTACCA GTGTCGCTGC CGTCCGGGCA CCTCCGGGGT CGACTGCGAG 
TACAACGTCA ACGAGTGCTT CTCCAACCCC TGCCGGCACG GAGCAAAGTG CATCGACGGG 
ATAGACTCCT ACACTTGCGA CTGCCTTGCC GGATTCACTG GCATTCACTG CGAGACCAAC 
ATCAACGAGT GCGCCAGCAA CCCCTGTTCT AATGGTGGCG TCTGCACCGA CCTGGTCAAC 
GGATTTAAGT GTGACTGCCC CCGCGGCTAC TTCGATGCTC GCTGCCTCTC CGACGTCAAC 
GAGTGCGCCA GCAACCCGTG CCAGAACGGA GGCACCTGTG AAGACGAGGT CAACCGCTTC 
GTCTGCCACT GCCCTCCCGG TTACGGGGGC CACCGCTGCG AGCAAGACAT AGACGAGTGC 
CAATCCAATC CGTGCCAGCA TGGTGGAAGC TGCCACGACA CCCTCAATGC TTACTCGTGC 
ACCTGCATAG ACGGCTACTC CGGGCGGAAT TGCGAGACGA ACCTTGACGA CTGCAGTCCT 
AATCCGTGCC TCAATGGCGG AAGTTGCATT GACCTGGTCG GCACGTTCCG CTGCGTGTGT 
GAGGTGCCCT TTTCCGGTCC CACATGCGAC GTCGAGTTGG ACCCCTGCTC TCCCAACAAG 
TGTCGCAACG GTGCCCAGTG TTCGCCGTCC TCGAATTACC TCGACTTTGC TTGCTCCTGC 
AAGTTGGGCT TCACGGGGAG GTTGTGCGAC GAGGACATCG ACGAGTGTGC GGTGTCGTCG 
CCGTGCCGCA ATGAGGCCAC GTGCGTCAAC GTAAACGGAT CGTACGAGTG CCTCTGCACC 
CGCGGCTACG AGGGTCGGGA CTGCCTCACC AACACCGACG ACTGCGCTTC GTACCCCTGC 
CAGAATGGAG GAACGTGTCT GGATGGCATT GGAGAGTACA CCTGTCTGTG CGTTGATGGC 
TTTGGCGGAG AGCACTGCGC CGACGACATG AACGAATGTG CCAGCAACCC CTGCCAGAAT 
GGAGCCACCT GCAATGACTA TGTGAACAGC TATGCCTGTG CCTGCCTCCC GGGCTTCAGC 
GGAACCAACT GCCAGACCAA CGATGAGGAC TGCACCCCAA GTTCCTGCAT GAACGGCGGC 
ACATGCGTGG ACGGAGTGAA CAACTACACG TGCCAGTGCA GTCCGGGCTA CACGGGCTCC 
AACTGCCAGT ACCACATCAA CGAGTGTGAC TCCCAGCCCT GTGCCCATGG GGCCACCTGT 
GTCCACCACA TTGGCTACCA CACCTGCCAC TGCCCCTTCG GGTACATGGG ACCACGCTGC 
GAGACCTTTG TGGACTGGTG TGCCACCAAC CCATGCCTCA ATGGAGCCAG CTGTAAGCAG 
TCCAACAACA CGTATCGCTG CACCTGCCAA CCTGGGTGGA CAGGCCTGCT CTGTGACGTG 
TCCATGGTGT CATGTGAAGA TGCTGCCCTG CAGAAAGGCA CCAAAGTGGC CGACCTCTGC 
AAGCACGGCG GAGTGTGCGA AGACTTCGGC AACAGCCACC ACTGCCTCTG CCCCGAAGGC 
TACGAGGGCA GCTACTGCCA GCGCGAAGTC AACGAGTGCC TGAGCAACCC GTGCCAGAAC 
GGAGCCACCT GCCACGACCT CCTGGGCCAG TATGCCTGTG ACTGCCCCGA GGGCTTCCAG 
GGGCTCAACT GCGAGTACGA CGTCAACGAC TGCGACCCTA GCCCCTGCCG CAATGGCGGC 
ACCTGCCACG ATCTCGTCAA CAAGTTTGTC TGCTCCTGCC CCCACGGCAC CCTCGGCACC 
CTCTGCGAGA TCGACGTCAA CGAGTGCTTT GAGAGCGCCT GCCACCACGG AGGCACCTGC 
CTCGACCGCG TCGGCAGGTA CGAGTGCCAA TGCCCGCCCG GGTACGTGGG CTCCCGCTGC 
GAAGGCGATG TCAACGAGTG CCTCTCGAGC CCCTGTGACC CGCTAGGGAC CCTAGACTGC 
GTGCAGCTCG TCAATGACTA CCGCTGCGAC TGCCGCCCGG GTCATGCGGG GCGCCGGTGC 
GAGCTCAAGG TCGACCCTTG CTCCACGGAC CCGTGCCTCA ACGGCGGCGT CTGCCACCCG 
GGGCCACGGG GTCTGACCTG CGTCTGCCAG GAGGGCTTCT GGGGCGAAAC CTGCGCCAAC 
CGCAGTTGTG GAGACACCAG CCCGTGCCGC AACGGGGGAC AGTGCCGCGG CCAGTCCTGC 
GTCTGTCCCC GCGGAACTTC CGGAAATTTC TGCGAGCGGC TGGAGGAGCT TGGTGCCGGT 
GGTGCCTGCC CGCTGGGGTG CCGCAACGGG GGAGCGTGCC GCAAGGGTGC AAGCGGGAGG 
TTCGAGTGTG AGTGCCTAGT TGGATGGAAG GGGCTGCGGT GCGAAGAATA CGACTCCAGC 
TTCCGCGGTG CCTTGGCGCC TTCAGTGGAG GGTGTTTATG ACCTCTTCCT CAAGTCTCTG 
GAAGAAGAGA AGAAGCTGTG CGCCGCTCGG AGGTGTGAGA AGAAGGCGGG GAACCGCTAC 
TGTGACGAAG AGTGCAACAC GTACGCCTGC AATTTTGATG GTGGTGACTG CTCTCTCGGA 
ATTAATCCTT GGATGAACTG CACTGCTGCC ATCAAGTGCT GGGACGTGTT CAGAGACGGA 
AAGTGCAATG TCGAGTGCAA CAATATCGAC TGCCTCTTTG ATGGCTTTGA TTGCCAGAAA 
GACCTCCAAC CTTGCAATGA GCACTATGAC ACCTACTGCA TGAGCAACTA TGGTAATGGC 
TACTGCGACT ACGGCTGCAA CAACGAGGAG TGCAACTGGG ACGGGCTCGA CTGTGAAATG 
GATCCCCCTT ACCTGGCGGA GGGTTCCATT GTCATCATGA TCTCCATCGA GCCAGAGGTG 
TTCCGGAACA ACACGGTGCC CTTCTTGCGT GACATTGGCC ACGCTCTGCG CACCAATGTG 
CTCTTCAAGC GGGACCCCAC AGGCAGCCCC ATGATCTACC CTTGGCGGGC CACCGATGGA 
CAGAAACCCA ACCACGCGGC ACTGCCCGTA CCGCGCACAG GGACGCTGGT GCACCTGGAG 
ATTGACAACC GGCGCTGCCT CAAGTCTCAG GGCAGCGAGT GCTTCCAGTC TGCGGCCAAG 
GCGGCAGAGT TCCTGGCGGC AGCCCAGGCC CGGCACTCCC TCGAGACCCC GTTCCACATC 
CAGGAGGTGC AGGGGGCCGG CCGGGAGCAA GGGGGCGACG GGGCGGGCGA CGGGGGCCGG 
GCCAGTGCCC TGCACGTGGT CATCGGCCTC ATCGGCGTGC TGCTGGCGGG CCTGCTGGTG 
GGCGTGCTGT TCACCACGCA GAAGAAGCGG GCCCGGGGCA TCACCTGGTT CCCCGAGGGC 
TTTCTCCGTA CCGGCAGCGG TCAAAGAAGG CACTCGAGGC GCAGGGGCCC CGACGGACAG 
GAGATGAGAG CGGCTCACGC CAATGGCGAG GGCCCTCCCG AAGCCTGGAG CGACGACGAA 
GGAGAGCGTC CACCGGCCAA ACGTGCACGC GGTTCCCCCG ACTCGAGCTG TGGAGACACG 
GTGGTGTCCG ACTGCGAGGA GGAGAGGGAC CCCCGACCCT GGACCCAGCA GCACCTGGAG 
GCTGCGGACG TGCATCACCC AGAGCTGCTG CTGGCCCTGA CCCCACCCCA GGGCAATGAT 
CTCGACCAGC ACAACGTGGA CGTCAGGGGC CCTGGTGGCC TCACCCCGCT AATGCTGGCC 
TCTTTTCGCG GGAATGGCCT GGACACTGGC GAAGATGTGG GCGAGGATGA CGACGGCTCT 
GCCAGCATGA TCCAGGATCT GCTCATGCAG GGAGCGCAGC TTGCCAGTAC CACCGAAAAG 
ACTGGCGAGA CTCCCCTGCA CTTGGCCGCA CGCTACGCTC GTGCGGATGC CGCCAAGCGC 
CTCCTCGACG CCGGTGCAGA GGCCAATGCG CAGGACAGTT CGGGCAGAAC GCCCCTGCAT 
GCCGCCATTG CTGCCGACGC CGTGGGAGTG TTCCAGATCT TGCTACGCAA TCGCGCCACC 
AACCTCAACG CCAAGATGAA CAATGGGATG ACGCCGCTCA TCCTCTCCAT CCGCCTGGCC 
ATGGAGGGAA TGGCCGAGGA CCTCATCAAC GCCGAGGCTG ACGTCAATGC GGCCGACTGC 
GACGCTCGCA CGCCCCTCCA CTGGGCCGCC GCCGTCAATG ACGTCACCTC CGTGCGCATC 
CTGCTCGCGC ACGGCGCAAA CCGCGACGCG CAAGACATTC ACGAAGAGAC TCCGCTGTTC 
TTAGCAGCCC GTGAGGGCAG CCTCAATGCG GTGAGGGCGC TGCTCGACCA CGCTGCCAAC 
CGAGACATCA CCAACCACAT GGACCGGCGG CCGCGTGACG TGGCTCGTGA CCGCATGCAC 
CTTGACATCG TCGACCTGCT CGACAAGTAC GTCCCGTCGT CCCAGATCCC CGGGCTGGGC 
AGCCCCCCTA TGCTGCTCTC CAATCCGACC GTCATTGGTT CCACCAAGTC GGCCAAGGGC 
AAGCGGCGCC AGCAGCCCAA GAACAATGGC AACGCGAGCA TCGCGCTGCA GCACCTTGAC 
GCGTCTGCGA CGTCACCCGG CGCGGAGGTC AACCATGTCA AGCGGCGGCC CTCCATCAAG 
AAGCGAAGGG AACCACCCAT GCCGTTGCCA GAGGTGCCCC ACCCGCTCGA CTCTCCGCAC 
GAATCTCCGG GTGGGCCGCT CTTCCTCCAG CAGGGCGGAG TGGCCACGCT GACCAAGCAG 
CCACCGTGCT ACGAGGACTG CGTGGGGCCC GTGTACACGG CCGTGTACGA CCCACAGGCC 
TATGCCAACG CCCCGATGGC CCAGCAGCAA CAGCACCCGC GACAGAACTC GGTCCCGTCC 
ATGGGTGGAT ACACGATGGC CTCTTCGCCT GCCAAGCAGC GCCCCTCGCT GCCCACGTCG 
CCCACCCACA TGGCGGCCAT GAGGGCCGCA CACCAGCACA AGCTGCAGCA GGGTGCCCCC 
ACGTATGACT ACCCCGTCCA GCCAGCCTAC TACCACTACC CTACGCCCCC GTCCCAGCAC 
AGCCATGGCG GCGGGCCCGA GGCCACGCCC CAGCACTACC TCCACCCGGC GGAGACTTAC 
CTGACGCCCT CGCCGGAGTC TCCAGGACAG TGGTCCAGCT CCTCGCCGCA CTCGGCGCAG 
TCCGACTGGT CGGAAGGGAT CTCCAGCCCC CTTGGGCAGC AGGCGCTTGG GGGATGCGAG 
CTCAAGCAGC AGCAGCAGCA GCAGACCGTC CAGCCGGCTG GACTGACCCA CCCGGATCCG 
GTGTTCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029845473.1
CDS	115..7086
Translation	MNPCQAVSGKRCQNGGTCNVEMSVTSGPSFSCTCPVGYSASMCEIAVPSACDDRPCRNGGTCSLVTLDNYTCACATGFRGKHCDKKDHCASQPCLNYGSCLSRADSYACVCAQGYSGPTCAVDVDECKAKPCEQGTCINTQGSFSCVCEAGFTGQLCESRYIPCEPSPCLNGGTCRPIDSLNYQCSCPPGFTGTHCEANVDDCPGNLCQNGATCLDGVNSYTCHCPPTYTGPYCSRDVDECSFRPTVCKNGATCTNTVGGYSCICVNGWTGTDCSENIDDCAVAACFNGATCHDRVGSFYCQCAPGKTGLLCHLDDACASNPCHEGAICDTSPIDGTYLCSCPNGFQGVDCTEDVDECAQGEPCEHGGICVNTPGSFRCNCTRGFAGPRCEVNINECDSNPCHNEGTCLDERGGFRCVCMPGYKGIHCEVDIDECLPNPCLNGGICNDLINGFKCLCPVGFSGKKCEANEDDCSSFPCRNGGSCHDGIASYTCHCPPGFTGATCETNINDCQSSPCHHGVCHDGINSFSCNCHAGYTGLLCQTQINECLSSPCRHGGTCEDLVNGYQCRCRPGTSGVDCEYNVNECFSNPCRHGAKCIDGIDSYTCDCLAGFTGIHCETNINECASNPCSNGGVCTDLVNGFKCDCPRGYFDARCLSDVNECASNPCQNGGTCEDEVNRFVCHCPPGYGGHRCEQDIDECQSNPCQHGGSCHDTLNAYSCTCIDGYSGRNCETNLDDCSPNPCLNGGSCIDLVGTFRCVCEVPFSGPTCDVELDPCSPNKCRNGAQCSPSSNYLDFACSCKLGFTGRLCDEDIDECAVSSPCRNEATCVNVNGSYECLCTRGYEGRDCLTNTDDCASYPCQNGGTCLDGIGEYTCLCVDGFGGEHCADDMNECASNPCQNGATCNDYVNSYACACLPGFSGTNCQTNDEDCTPSSCMNGGTCVDGVNNYTCQCSPGYTGSNCQYHINECDSQPCAHGATCVHHIGYHTCHCPFGYMGPRCETFVDWCATNPCLNGASCKQSNNTYRCTCQPGWTGLLCDVSMVSCEDAALQKGTKVADLCKHGGVCEDFGNSHHCLCPEGYEGSYCQREVNECLSNPCQNGATCHDLLGQYACDCPEGFQGLNCEYDVNDCDPSPCRNGGTCHDLVNKFVCSCPHGTLGTLCEIDVNECFESACHHGGTCLDRVGRYECQCPPGYVGSRCEGDVNECLSSPCDPLGTLDCVQLVNDYRCDCRPGHAGRRCELKVDPCSTDPCLNGGVCHPGPRGLTCVCQEGFWGETCANRSCGDTSPCRNGGQCRGQSCVCPRGTSGNFCERLEELGAGGACPLGCRNGGACRKGASGRFECECLVGWKGLRCEEYDSSFRGALAPSVEGVYDLFLKSLEEEKKLCAARRCEKKAGNRYCDEECNTYACNFDGGDCSLGINPWMNCTAAIKCWDVFRDGKCNVECNNIDCLFDGFDCQKDLQPCNEHYDTYCMSNYGNGYCDYGCNNEECNWDGLDCEMDPPYLAEGSIVIMISIEPEVFRNNTVPFLRDIGHALRTNVLFKRDPTGSPMIYPWRATDGQKPNHAALPVPRTGTLVHLEIDNRRCLKSQGSECFQSAAKAAEFLAAAQARHSLETPFHIQEVQGAGREQGGDGAGDGGRASALHVVIGLIGVLLAGLLVGVLFTTQKKRARGITWFPEGFLRTGSGQRRHSRRRGPDGQEMRAAHANGEGPPEAWSDDEGERPPAKRARGSPDSSCGDTVVSDCEEERDPRPWTQQHLEAADVHHPELLLALTPPQGNDLDQHNVDVRGPGGLTPLMLASFRGNGLDTGEDVGEDDDGSASMIQDLLMQGAQLASTTEKTGETPLHLAARYARADAAKRLLDAGAEANAQDSSGRTPLHAAIAADAVGVFQILLRNRATNLNAKMNNGMTPLILSIRLAMEGMAEDLINAEADVNAADCDARTPLHWAAAVNDVTSVRILLAHGANRDAQDIHEETPLFLAAREGSLNAVRALLDHAANRDITNHMDRRPRDVARDRMHLDIVDLLDKYVPSSQIPGLGSPPMLLSNPTVIGSTKSAKGKRRQQPKNNGNASIALQHLDASATSPGAEVNHVKRRPSIKKRREPPMPLPEVPHPLDSPHESPGGPLFLQQGGVATLTKQPPCYEDCVGPVYTAVYDPQAYANAPMAQQQQHPRQNSVPSMGGYTMASSPAKQRPSLPTSPTHMAAMRAAHQHKLQQGAPTYDYPVQPAYYHYPTPPSQHSHGGGPEATPQHYLHPAETYLTPSPESPGQWSSSSPHSAQSDWSEGISSPLGQQALGGCELKQQQQQQTVQPAGLTHPDPVFI

Target ORF information:

RefSeq Version	XM_029989613.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis neurogenic locus Notch protein (LOC8030922), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029989613.1

ORF Insert Sequence:

ATGAACCCCT GCCAGGCCGT GTCGGGCAAG CGGTGTCAGA ACGGCGGCAC GTGCAACGTC 
GAGATGAGCG TGACCAGCGG GCCGAGCTTC TCGTGCACCT GTCCCGTGGG CTACTCGGCC 
AGCATGTGCG AGATCGCCGT GCCCAGCGCC TGCGACGACC GGCCCTGCCG CAACGGCGGC 
ACCTGTTCCC TCGTCACCCT CGACAACTAC ACGTGCGCTT GCGCCACGGG CTTCAGGGGC 
AAGCACTGCG ACAAGAAGGA CCATTGCGCG TCTCAGCCGT GCCTCAACTA CGGCAGCTGC 
CTGTCCAGGG CGGACTCCTA CGCCTGCGTG TGTGCCCAGG GATACAGCGG CCCCACGTGC 
GCCGTCGACG TGGACGAGTG CAAGGCAAAG CCCTGCGAGC AAGGCACTTG CATCAACACC 
CAGGGATCCT TCAGCTGTGT CTGCGAGGCG GGCTTCACGG GCCAGCTGTG CGAGTCCCGG 
TACATCCCGT GCGAGCCGAG CCCCTGCCTG AACGGCGGGA CCTGCAGACC CATCGACTCG 
CTCAACTACC AGTGCTCGTG TCCTCCTGGT TTCACGGGCA CCCACTGCGA GGCGAACGTA 
GACGACTGTC CGGGCAACCT GTGCCAGAAT GGCGCCACCT GCCTGGACGG AGTCAACTCG 
TACACCTGCC ACTGTCCGCC CACGTACACC GGGCCGTACT GCAGCCGCGA CGTGGACGAG 
TGCTCGTTCC GGCCAACGGT GTGCAAGAAC GGCGCCACGT GCACCAACAC CGTGGGCGGA 
TACTCGTGCA TCTGCGTCAA CGGATGGACG GGCACCGACT GCTCCGAGAA CATCGACGAC 
TGCGCTGTGG CGGCCTGCTT CAACGGCGCC ACGTGCCACG ACCGCGTCGG GTCCTTCTAC 
TGCCAGTGCG CTCCTGGGAA GACAGGGCTG CTGTGTCACC TGGACGACGC GTGCGCGAGC 
AACCCGTGCC ACGAGGGCGC CATCTGCGAC ACAAGCCCCA TCGACGGCAC CTACCTGTGC 
TCGTGCCCCA ACGGCTTCCA GGGCGTGGAC TGCACCGAGG ACGTGGACGA GTGCGCCCAG 
GGGGAGCCCT GCGAGCACGG CGGTATCTGC GTCAACACGC CGGGCTCGTT CCGCTGCAAC 
TGCACCCGGG GCTTCGCCGG GCCGCGCTGC GAGGTGAACA TCAACGAGTG CGACTCCAAC 
CCCTGCCACA ACGAGGGAAC CTGCCTGGAC GAGCGCGGAG GCTTCCGATG CGTCTGCATG 
CCCGGCTACA AGGGCATTCA CTGCGAAGTG GATATCGACG AGTGCCTCCC CAACCCATGC 
CTCAACGGAG GTATCTGCAA CGATCTCATC AATGGCTTCA AGTGCCTGTG TCCTGTGGGT 
TTCTCGGGCA AGAAGTGTGA AGCCAACGAA GACGATTGCT CTTCCTTCCC GTGCCGCAAT 
GGAGGCTCCT GCCATGATGG CATTGCCAGC TATACTTGCC ACTGTCCACC TGGCTTCACG 
GGTGCCACGT GTGAGACGAA CATCAACGAC TGCCAGTCTT CGCCGTGCCA CCATGGCGTC 
TGCCACGACG GCATCAACTC CTTCAGCTGC AACTGCCACG CGGGCTACAC GGGGCTGCTC 
TGCCAGACCC AGATCAACGA GTGCCTAAGC AGTCCCTGCC GCCACGGCGG GACATGCGAG 
GACCTCGTCA ACGGCTACCA GTGTCGCTGC CGTCCGGGCA CCTCCGGGGT CGACTGCGAG 
TACAACGTCA ACGAGTGCTT CTCCAACCCC TGCCGGCACG GAGCAAAGTG CATCGACGGG 
ATAGACTCCT ACACTTGCGA CTGCCTTGCC GGATTCACTG GCATTCACTG CGAGACCAAC 
ATCAACGAGT GCGCCAGCAA CCCCTGTTCT AATGGTGGCG TCTGCACCGA CCTGGTCAAC 
GGATTTAAGT GTGACTGCCC CCGCGGCTAC TTCGATGCTC GCTGCCTCTC CGACGTCAAC 
GAGTGCGCCA GCAACCCGTG CCAGAACGGA GGCACCTGTG AAGACGAGGT CAACCGCTTC 
GTCTGCCACT GCCCTCCCGG TTACGGGGGC CACCGCTGCG AGCAAGACAT AGACGAGTGC 
CAATCCAATC CGTGCCAGCA TGGTGGAAGC TGCCACGACA CCCTCAATGC TTACTCGTGC 
ACCTGCATAG ACGGCTACTC CGGGCGGAAT TGCGAGACGA ACCTTGACGA CTGCAGTCCT 
AATCCGTGCC TCAATGGCGG AAGTTGCATT GACCTGGTCG GCACGTTCCG CTGCGTGTGT 
GAGGTGCCCT TTTCCGGTCC CACATGCGAC GTCGAGTTGG ACCCCTGCTC TCCCAACAAG 
TGTCGCAACG GTGCCCAGTG TTCGCCGTCC TCGAATTACC TCGACTTTGC TTGCTCCTGC 
AAGTTGGGCT TCACGGGGAG GTTGTGCGAC GAGGACATCG ACGAGTGTGC GGTGTCGTCG 
CCGTGCCGCA ATGAGGCCAC GTGCGTCAAC GTAAACGGAT CGTACGAGTG CCTCTGCACC 
CGCGGCTACG AGGGTCGGGA CTGCCTCACC AACACCGACG ACTGCGCTTC GTACCCCTGC 
CAGAATGGAG GAACGTGTCT GGATGGCATT GGAGAGTACA CCTGTCTGTG CGTTGATGGC 
TTTGGCGGAG AGCACTGCGC CGACGACATG AACGAATGTG CCAGCAACCC CTGCCAGAAT 
GGAGCCACCT GCAATGACTA TGTGAACAGC TATGCCTGTG CCTGCCTCCC GGGCTTCAGC 
GGAACCAACT GCCAGACCAA CGATGAGGAC TGCACCCCAA GTTCCTGCAT GAACGGCGGC 
ACATGCGTGG ACGGAGTGAA CAACTACACG TGCCAGTGCA GTCCGGGCTA CACGGGCTCC 
AACTGCCAGT ACCACATCAA CGAGTGTGAC TCCCAGCCCT GTGCCCATGG GGCCACCTGT 
GTCCACCACA TTGGCTACCA CACCTGCCAC TGCCCCTTCG GGTACATGGG ACCACGCTGC 
GAGACCTTTG TGGACTGGTG TGCCACCAAC CCATGCCTCA ATGGAGCCAG CTGTAAGCAG 
TCCAACAACA CGTATCGCTG CACCTGCCAA CCTGGGTGGA CAGGCCTGCT CTGTGACGTG 
TCCATGGTGT CATGTGAAGA TGCTGCCCTG CAGAAAGGCA CCAAAGTGGC CGACCTCTGC 
AAGCACGGCG GAGTGTGCGA AGACTTCGGC AACAGCCACC ACTGCCTCTG CCCCGAAGGC 
TACGAGGGCA GCTACTGCCA GCGCGAAGTC AACGAGTGCC TGAGCAACCC GTGCCAGAAC 
GGAGCCACCT GCCACGACCT CCTGGGCCAG TATGCCTGTG ACTGCCCCGA GGGCTTCCAG 
GGGCTCAACT GCGAGTACGA CGTCAACGAC TGCGACCCTA GCCCCTGCCG CAATGGCGGC 
ACCTGCCACG ATCTCGTCAA CAAGTTTGTC TGCTCCTGCC CCCACGGCAC CCTCGGCACC 
CTCTGCGAGA TCGACGTCAA CGAGTGCTTT GAGAGCGCCT GCCACCACGG AGGCACCTGC 
CTCGACCGCG TCGGCAGGTA CGAGTGCCAA TGCCCGCCCG GGTACGTGGG CTCCCGCTGC 
GAAGGCGATG TCAACGAGTG CCTCTCGAGC CCCTGTGACC CGCTAGGGAC CCTAGACTGC 
GTGCAGCTCG TCAATGACTA CCGCTGCGAC TGCCGCCCGG GTCATGCGGG GCGCCGGTGC 
GAGCTCAAGG TCGACCCTTG CTCCACGGAC CCGTGCCTCA ACGGCGGCGT CTGCCACCCG 
GGGCCACGGG GTCTGACCTG CGTCTGCCAG GAGGGCTTCT GGGGCGAAAC CTGCGCCAAC 
CGCAGTTGTG GAGACACCAG CCCGTGCCGC AACGGGGGAC AGTGCCGCGG CCAGTCCTGC 
GTCTGTCCCC GCGGAACTTC CGGAAATTTC TGCGAGCGGC TGGAGGAGCT TGGTGCCGGT 
GGTGCCTGCC CGCTGGGGTG CCGCAACGGG GGAGCGTGCC GCAAGGGTGC AAGCGGGAGG 
TTCGAGTGTG AGTGCCTAGT TGGATGGAAG GGGCTGCGGT GCGAAGAATA CGACTCCAGC 
TTCCGCGGTG CCTTGGCGCC TTCAGTGGAG GGTGTTTATG ACCTCTTCCT CAAGTCTCTG 
GAAGAAGAGA AGAAGCTGTG CGCCGCTCGG AGGTGTGAGA AGAAGGCGGG GAACCGCTAC 
TGTGACGAAG AGTGCAACAC GTACGCCTGC AATTTTGATG GTGGTGACTG CTCTCTCGGA 
ATTAATCCTT GGATGAACTG CACTGCTGCC ATCAAGTGCT GGGACGTGTT CAGAGACGGA 
AAGTGCAATG TCGAGTGCAA CAATATCGAC TGCCTCTTTG ATGGCTTTGA TTGCCAGAAA 
GACCTCCAAC CTTGCAATGA GCACTATGAC ACCTACTGCA TGAGCAACTA TGGTAATGGC 
TACTGCGACT ACGGCTGCAA CAACGAGGAG TGCAACTGGG ACGGGCTCGA CTGTGAAATG 
GATCCCCCTT ACCTGGCGGA GGGTTCCATT GTCATCATGA TCTCCATCGA GCCAGAGGTG 
TTCCGGAACA ACACGGTGCC CTTCTTGCGT GACATTGGCC ACGCTCTGCG CACCAATGTG 
CTCTTCAAGC GGGACCCCAC AGGCAGCCCC ATGATCTACC CTTGGCGGGC CACCGATGGA 
CAGAAACCCA ACCACGCGGC ACTGCCCGTA CCGCGCACAG GGACGCTGGT GCACCTGGAG 
ATTGACAACC GGCGCTGCCT CAAGTCTCAG GGCAGCGAGT GCTTCCAGTC TGCGGCCAAG 
GCGGCAGAGT TCCTGGCGGC AGCCCAGGCC CGGCACTCCC TCGAGACCCC GTTCCACATC 
CAGGAGGTGC AGGGGGCCGG CCGGGAGCAA GGGGGCGACG GGGCGGGCGA CGGGGGCCGG 
GCCAGTGCCC TGCACGTGGT CATCGGCCTC ATCGGCGTGC TGCTGGCGGG CCTGCTGGTG 
GGCGTGCTGT TCACCACGCA GAAGAAGCGG GCCCGGGGCA TCACCTGGTT CCCCGAGGGC 
TTTCTCCGTA CCGGCAGCGG TCAAAGAAGG CACTCGAGGC GCAGGGGCCC CGACGGACAG 
GAGATGAGAG CGGCTCACGC CAATGGCGAG GGCCCTCCCG AAGCCTGGAG CGACGACGAA 
GGAGAGCGTC CACCGGCCAA ACGTGCACGC GGTTCCCCCG ACTCGAGCTG TGGAGACACG 
GTGGTGTCCG ACTGCGAGGA GGAGAGGGAC CCCCGACCCT GGACCCAGCA GCACCTGGAG 
GCTGCGGACG TGCATCACCC AGAGCTGCTG CTGGCCCTGA CCCCACCCCA GGGCAATGAT 
CTCGACCAGC ACAACGTGGA CGTCAGGGGC CCTGGTGGCC TCACCCCGCT AATGCTGGCC 
TCTTTTCGCG GGAATGGCCT GGACACTGGC GAAGATGTGG GCGAGGATGA CGACGGCTCT 
GCCAGCATGA TCCAGGATCT GCTCATGCAG GGAGCGCAGC TTGCCAGTAC CACCGAAAAG 
ACTGGCGAGA CTCCCCTGCA CTTGGCCGCA CGCTACGCTC GTGCGGATGC CGCCAAGCGC 
CTCCTCGACG CCGGTGCAGA GGCCAATGCG CAGGACAGTT CGGGCAGAAC GCCCCTGCAT 
GCCGCCATTG CTGCCGACGC CGTGGGAGTG TTCCAGATCT TGCTACGCAA TCGCGCCACC 
AACCTCAACG CCAAGATGAA CAATGGGATG ACGCCGCTCA TCCTCTCCAT CCGCCTGGCC 
ATGGAGGGAA TGGCCGAGGA CCTCATCAAC GCCGAGGCTG ACGTCAATGC GGCCGACTGC 
GACGCTCGCA CGCCCCTCCA CTGGGCCGCC GCCGTCAATG ACGTCACCTC CGTGCGCATC 
CTGCTCGCGC ACGGCGCAAA CCGCGACGCG CAAGACATTC ACGAAGAGAC TCCGCTGTTC 
TTAGCAGCCC GTGAGGGCAG CCTCAATGCG GTGAGGGCGC TGCTCGACCA CGCTGCCAAC 
CGAGACATCA CCAACCACAT GGACCGGCGG CCGCGTGACG TGGCTCGTGA CCGCATGCAC 
CTTGACATCG TCGACCTGCT CGACAAGTAC GTCCCGTCGT CCCAGATCCC CGGGCTGGGC 
AGCCCCCCTA TGCTGCTCTC CAATCCGACC GTCATTGGTT CCACCAAGTC GGCCAAGGGC 
AAGCGGCGCC AGCAGCCCAA GAACAATGGC AACGCGAGCA TCGCGCTGCA GCACCTTGAC 
GCGTCTGCGA CGTCACCCGG CGCGGAGGTC AACCATGTCA AGCGGCGGCC CTCCATCAAG 
AAGCGAAGGG AACCACCCAT GCCGTTGCCA GAGGTGCCCC ACCCGCTCGA CTCTCCGCAC 
GAATCTCCGG GTGGGCCGCT CTTCCTCCAG CAGGGCGGAG TGGCCACGCT GACCAAGCAG 
CCACCGTGCT ACGAGGACTG CGTGGGGCCC GTGTACACGG CCGTGTACGA CCCACAGGCC 
TATGCCAACG CCCCGATGGC CCAGCAGCAA CAGCACCCGC GACAGAACTC GGTCCCGTCC 
ATGGGTGGAT ACACGATGGC CTCTTCGCCT GCCAAGCAGC GCCCCTCGCT GCCCACGTCG 
CCCACCCACA TGGCGGCCAT GAGGGCCGCA CACCAGCACA AGCTGCAGCA GGGTGCCCCC 
ACGTATGACT ACCCCGTCCA GCCAGCCTAC TACCACTACC CTACGCCCCC GTCCCAGCAC 
AGCCATGGCG GCGGGCCCGA GGCCACGCCC CAGCACTACC TCCACCCGGC GGAGACTTAC 
CTGACGCCCT CGCCGGAGTC TCCAGGACAG TGGTCCAGCT CCTCGCCGCA CTCGGCGCAG 
TCCGACTGGT CGGAAGGGAT CTCCAGCCCC CTTGGGCAGC AGGCGCTTGG GGGATGCGAG 
CTCAAGCAGC AGCAGCAGCA GCAGACCGTC CAGCCGGCTG GACTGACCCA CCCGGATCCG 
GTGTTCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IscW_ISCW007213 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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