IscW_ISCW004026 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	IscW_ISCW004026
Entrez Gene ID	8029418
Full Name	phosphatidylinositol 3-kinase class, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029981483.1	XP_029837343.1	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
XM_002404883.1	XP_002404927.1	phosphatidylinositol 3-kinase class, putative

Pathways from BioSystems
Gene Ontology

The following IscW_ISCW004026 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IscW_ISCW004026 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIa03536	XM_002404883.1 Latest version!	Ixodes scapularis phosphatidylinositol 3-kinase class, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OIa24288	XM_029981483.1 Latest version!	Ixodes scapularis phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (LOC8029418), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IscW_ISCW004026 ( XM_002404883.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002404927.1 Ixodes scapularis phosphatidylinositol 3-kinase class, putative, mRNA.

Price & Availability↑

CloneID	OIa03536
Clone ID Related Accession (Same CDS sequence)	XM_002404883.1
Accession Version	XM_002404883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	phosphatidylinositol 3-kinase class, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002611764). COMPLETENESS: incomplete on the 5' end.

ATGTCCCACT TCTTCAAGTT TGTGCAGGCA AGGCTGGTCA AGAAACAGCC AAACCACCAC 
AGCCAGAGGC TCCTGAGGAA ACTGCAGCAT CTCGTGTGTG ACGTGCAACC AGAAGACAGT 
CCTTATTCGG GGCTTCTCAT CAGCCCTACG TTGCAAAACA ACTCGGAAGA GACATTCGAC 
GTCAAGCTCA AAATCTTCTC TGAACAACTC CACAAGCCAG TGTGTTTCTC TTGTCCGGCA 
GCTACAAGGG TGGGCTACCT TGTGGAAAGC ATCCAGCCAT TCCTGGGCAG TCCCGGAAAG 
TATGTCTTCA AGGTGACCGG CCTCGATGAG TACTTGTCCA ACGATACCCT ACTCAGCGAC 
TATGCATACA TCTGCGCCTG TAGACGTGTC GGCAAAGTGG CCTGGTTGAC TATTGTAAAT 
CTGGACAATG TGCAGAAGCC ATGGACATTA GTGACCAGCG AGAGTTGTGA TTTGGACATT 
GATGTCTCGG AGAAGATAAC GTTTGTTGCC GTCAGGGATG CCTTGGAGAC TTTCGAGTCC 
CAGGTAGAAG AGCTCATAGG CGTCGCTCTG TCGGTTGGCG GAGATCTCGT CAAGGCAGCA 
GGGGACATCA GAGCCAGTGT GACAGAACTT TGCCAGTTGT TGAACCACAC GGAGACAAGT 
GAACTGTACC AGACAACTAC AGACCTCTTA AATTTTTTCA GAGACACGCT GTCAGAGAAT 
AGAGATGGTG TCGACGCCCC CAGAAAGGTG TTCCTTGTGA CACGGTTTGT TGAAGACATT 
GAACTTGCAA TTGCAAGGTT CCTTGCAATG CATTCTCAAA GGCGTAGCGT TGAATTCACG 
GTTGAGTTTC TCGATGAGAG CTTACGACGA AGACGCACCG TAGATGCTAA CGAACTGTTC 
AGCCCCAAGG TTGAGATGCT GGTTCAGCTG CAGAAAGCTT GGGTCCAAAA CTATGACAGC 
TACAGTGTGG ACTGCGCGTT AACCTATGGC GAAAGACTCT TTAGTTATCA GACGACACGA 
CGGTTGTATG TGACGCAAAG AATTTTCCAA TGTGTAAACT TTGACGAGTA CATTTCCTTC 
AAAAACATTC AGATTCGATC CCTTCCCCGA GAAACGAGGA TGTATCTGAC GGTCGTTGGG 
CACCGAGATG GAGCTGATCA CGTCCTTGGT TGGACAGGTC TGAACATCTT TGCAGACCGA 
GAGCACTTCA ATGGAGGCGA GTTCCTCCTT GGCCTATGGT CCCCTGAAAT GCTGTCGACA 
CGCGGTCCCG TTTCCGCAAG CGAGCAGAAC AAAGGATGTT TGCTAGTCAC ACTGCCACAT 
GTCAAGAACC AGGCAGTCCT CGCCAAATCC ATCACCAGAA ACGTGCGCGA AGTTGTCAGA 
AAGCCGTTTC ATGAACTCAG TGCCGACGTG CAGCAAACTT TCCTGGACCT GACCGTAACT 
GATGTCTCCA CGCCCTTGCC CACTTATGTT CGCGACCTGA TCTGGGAGCA CCGGCACCAC 
CTCTGTGACT TCTCAAGCTT GCTGCCTCAT TTGCTGAAGC TCCCGGTATG CTGGAATTCA 
GCAAACATTG AGGAGACCTA CACCCTGGTG CGGTCTTGGT GTCCACTCAG TCCGGAAGAG 
TCGCTTACGT TGCTCTCCCC CGCCTTCCCA GACAGCCTGG TCAAGAAGTA TGCGGTGCAA 
TCCCTGCAAA GCGTCGGCAG TGACGAGCTC TCCCTCTACC TTGCGCAGCT GGTCGAGTCT 
CTGAGGTTCC AAAATTGGGA CGACTCCTTC TTGGCCACGT TCCTCCTGAG GAGGGCCCGG 
CAGAGCGTCC GCCTCGCTCA TCAGCTCTTT TGGTGGCTCA CTGGCATGAT CAACGAACCG 
CTCATGAGGG ACAGGGCGCT GATCATGCGG AAGGCCCTCT TCTTTGTAGC AGGAGAGAAG 
GTCACGGCCA GCCTCCTGAC GCAGATCGTC GCAGACGAAA TGCTAGCCGA AACCGCGCGG 
AGCGTCAAGC AAGCAGCCGA CTCACAAAAA GAGGAGGTGA TGCGAAGAGA GCTTGCCAAG 
CTCAACAGCT CTTTGCAGGT GCTGAAGTTG AGGCTACCTC TGGACCCGGC AATAAGCGTC 
GAGTCCGTCG ATGTGGACAC CTGCTGCGTG TTTCCTTCCA AGACACTGCC CGTCAAGCTT 
GTCTTCCGGT CGTCCGAAGA AGCTGCCGAA AAGATTGAAA CAATCTACAA GGCTGGCGAC 
GACCTCCGTC AAGACGTTCT CATACTCACC GTGATTCGCC TCATGGACAA GCTTTGGCTG 
GAGAGCGGAA TGGACCTCAA GATGCTCACG TTCAAGTGCA CAACAACGGG AGAAAGCAGA 
GGCTTCCTCG AAGCCGTCCG GGATGTGTCG ACGCTACGGA CCATCCAGAC TAATCACTCG 
GGCGTCACGG GAGCGTTCAG CAACAGGACA ATTGCCGACT GGCTTTGGAA ACACAACTCG 
TGTAGGCCGC AGTACCAGGC GGCTGTCGAC AACTTCATCA AGTCCTGCGC CGGTTGCTGC 
GTCGCCACAT ACGTCCTGGG CGTCGGCGAC CGCCACAACG ACAACATCCT TCTGGCCACG 
TCGGGCCACA TGCTGCACAT TGATTTCTCG AAGGTCCTGG GCCATGCCGA GACGTTTGGG 
GGAATCAGGA GAGACAGGGC TCCGTTTGCA CTCACGCAAG ACATGGTCTA CGTCATCCGG 
AGGAACGGCG GAGAGGCACA GTTTGTGAGA CTCTGCTGCG AAGCCTACAA CGTCCTGCGA 
CGCAACTCCA GCCTTTTCCT CGCCCTCTTC AGCATCATGA AGCACTCGGG GCTGCCCGAC 
GTCGACCAGG ACGCGCTCGC ATACCTGCGA CGGTCCATGT CGCTGGAGGC GACGGACGAG 
CAGGCGGCGC GGCACTTTGA GAAGCGCATC CGCGAGAGCG TCATGACCCT CGCCACCAGC 
TTCAACTTCT TCATCCACAA CCTGGCGAGC CTGGGAGCCA ACACCAGGGA GTGCGCGCTG 
AGGCTCTCCT TCAACCCCCG TGACTTCAGC ATTGCTGCCG AGGGGAAGAT TGTGCAGCTG 
ACTGTGGAGG ACATCAGGCT GCGCTACCAT CCCGAGCGCT ACTACGTCTA TGTTGTCAAC 
GTGGAACGCG AGTTCCCACC GGCGCAGTCG GTCATCAGGA CCTACAAGAA CTTCCTCGAA 
CTGTACACCA AGATGAGTCG CAAGTTTCCC GCTTGCGACT TTCACCCGCT CTCGAGAGGT 
AGTGTCTTCA GCTCTGAAGG GGACGAGTCC CTTGCTCTCA AGCGCAAGCG AGACATCCAA 
CAGTTTCTGG ACACGGTGAT GGCTCAGCCG GAAGACGTGT CACACTACAG GCTCGTGTAC 
AGCTTCTTCC ATCCCCTGCC GCGAGACCTC GAACTTTTGA ACGGGGTTCC GATCACCATT 
GCCACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002404927.1
CDS	1..3429
Translation	MSHFFKFVQARLVKKQPNHHSQRLLRKLQHLVCDVQPEDSPYSGLLISPTLQNNSEETFDVKLKIFSEQLHKPVCFSCPAATRVGYLVESIQPFLGSPGKYVFKVTGLDEYLSNDTLLSDYAYICACRRVGKVAWLTIVNLDNVQKPWTLVTSESCDLDIDVSEKITFVAVRDALETFESQVEELIGVALSVGGDLVKAAGDIRASVTELCQLLNHTETSELYQTTTDLLNFFRDTLSENRDGVDAPRKVFLVTRFVEDIELAIARFLAMHSQRRSVEFTVEFLDESLRRRRTVDANELFSPKVEMLVQLQKAWVQNYDSYSVDCALTYGERLFSYQTTRRLYVTQRIFQCVNFDEYISFKNIQIRSLPRETRMYLTVVGHRDGADHVLGWTGLNIFADREHFNGGEFLLGLWSPEMLSTRGPVSASEQNKGCLLVTLPHVKNQAVLAKSITRNVREVVRKPFHELSADVQQTFLDLTVTDVSTPLPTYVRDLIWEHRHHLCDFSSLLPHLLKLPVCWNSANIEETYTLVRSWCPLSPEESLTLLSPAFPDSLVKKYAVQSLQSVGSDELSLYLAQLVESLRFQNWDDSFLATFLLRRARQSVRLAHQLFWWLTGMINEPLMRDRALIMRKALFFVAGEKVTASLLTQIVADEMLAETARSVKQAADSQKEEVMRRELAKLNSSLQVLKLRLPLDPAISVESVDVDTCCVFPSKTLPVKLVFRSSEEAAEKIETIYKAGDDLRQDVLILTVIRLMDKLWLESGMDLKMLTFKCTTTGESRGFLEAVRDVSTLRTIQTNHSGVTGAFSNRTIADWLWKHNSCRPQYQAAVDNFIKSCAGCCVATYVLGVGDRHNDNILLATSGHMLHIDFSKVLGHAETFGGIRRDRAPFALTQDMVYVIRRNGGEAQFVRLCCEAYNVLRRNSSLFLALFSIMKHSGLPDVDQDALAYLRRSMSLEATDEQAARHFEKRIRESVMTLATSFNFFIHNLASLGANTRECALRLSFNPRDFSIAAEGKIVQLTVEDIRLRYHPERYYVYVVNVEREFPPAQSVIRTYKNFLELYTKMSRKFPACDFHPLSRGSVFSSEGDESLALKRKRDIQQFLDTVMAQPEDVSHYRLVYSFFHPLPRDLELLNGVPITIAT

Target ORF information:

RefSeq Version	XM_002404883.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis phosphatidylinositol 3-kinase class, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002404883.1

ORF Insert Sequence:

ATGTCCCACT TCTTCAAGTT TGTGCAGGCA AGGCTGGTCA AGAAACAGCC AAACCACCAC 
AGCCAGAGGC TCCTGAGGAA ACTGCAGCAT CTCGTGTGTG ACGTGCAACC AGAAGACAGT 
CCTTATTCGG GGCTTCTCAT CAGCCCTACG TTGCAAAACA ACTCGGAAGA GACATTCGAC 
GTCAAGCTCA AAATCTTCTC TGAACAACTC CACAAGCCAG TGTGTTTCTC TTGTCCGGCA 
GCTACAAGGG TGGGCTACCT TGTGGAAAGC ATCCAGCCAT TCCTGGGCAG TCCCGGAAAG 
TATGTCTTCA AGGTGACCGG CCTCGATGAG TACTTGTCCA ACGATACCCT ACTCAGCGAC 
TATGCATACA TCTGCGCCTG TAGACGTGTC GGCAAAGTGG CCTGGTTGAC TATTGTAAAT 
CTGGACAATG TGCAGAAGCC ATGGACATTA GTGACCAGCG AGAGTTGTGA TTTGGACATT 
GATGTCTCGG AGAAGATAAC GTTTGTTGCC GTCAGGGATG CCTTGGAGAC TTTCGAGTCC 
CAGGTAGAAG AGCTCATAGG CGTCGCTCTG TCGGTTGGCG GAGATCTCGT CAAGGCAGCA 
GGGGACATCA GAGCCAGTGT GACAGAACTT TGCCAGTTGT TGAACCACAC GGAGACAAGT 
GAACTGTACC AGACAACTAC AGACCTCTTA AATTTTTTCA GAGACACGCT GTCAGAGAAT 
AGAGATGGTG TCGACGCCCC CAGAAAGGTG TTCCTTGTGA CACGGTTTGT TGAAGACATT 
GAACTTGCAA TTGCAAGGTT CCTTGCAATG CATTCTCAAA GGCGTAGCGT TGAATTCACG 
GTTGAGTTTC TCGATGAGAG CTTACGACGA AGACGCACCG TAGATGCTAA CGAACTGTTC 
AGCCCCAAGG TTGAGATGCT GGTTCAGCTG CAGAAAGCTT GGGTCCAAAA CTATGACAGC 
TACAGTGTGG ACTGCGCGTT AACCTATGGC GAAAGACTCT TTAGTTATCA GACGACACGA 
CGGTTGTATG TGACGCAAAG AATTTTCCAA TGTGTAAACT TTGACGAGTA CATTTCCTTC 
AAAAACATTC AGATTCGATC CCTTCCCCGA GAAACGAGGA TGTATCTGAC GGTCGTTGGG 
CACCGAGATG GAGCTGATCA CGTCCTTGGT TGGACAGGTC TGAACATCTT TGCAGACCGA 
GAGCACTTCA ATGGAGGCGA GTTCCTCCTT GGCCTATGGT CCCCTGAAAT GCTGTCGACA 
CGCGGTCCCG TTTCCGCAAG CGAGCAGAAC AAAGGATGTT TGCTAGTCAC ACTGCCACAT 
GTCAAGAACC AGGCAGTCCT CGCCAAATCC ATCACCAGAA ACGTGCGCGA AGTTGTCAGA 
AAGCCGTTTC ATGAACTCAG TGCCGACGTG CAGCAAACTT TCCTGGACCT GACCGTAACT 
GATGTCTCCA CGCCCTTGCC CACTTATGTT CGCGACCTGA TCTGGGAGCA CCGGCACCAC 
CTCTGTGACT TCTCAAGCTT GCTGCCTCAT TTGCTGAAGC TCCCGGTATG CTGGAATTCA 
GCAAACATTG AGGAGACCTA CACCCTGGTG CGGTCTTGGT GTCCACTCAG TCCGGAAGAG 
TCGCTTACGT TGCTCTCCCC CGCCTTCCCA GACAGCCTGG TCAAGAAGTA TGCGGTGCAA 
TCCCTGCAAA GCGTCGGCAG TGACGAGCTC TCCCTCTACC TTGCGCAGCT GGTCGAGTCT 
CTGAGGTTCC AAAATTGGGA CGACTCCTTC TTGGCCACGT TCCTCCTGAG GAGGGCCCGG 
CAGAGCGTCC GCCTCGCTCA TCAGCTCTTT TGGTGGCTCA CTGGCATGAT CAACGAACCG 
CTCATGAGGG ACAGGGCGCT GATCATGCGG AAGGCCCTCT TCTTTGTAGC AGGAGAGAAG 
GTCACGGCCA GCCTCCTGAC GCAGATCGTC GCAGACGAAA TGCTAGCCGA AACCGCGCGG 
AGCGTCAAGC AAGCAGCCGA CTCACAAAAA GAGGAGGTGA TGCGAAGAGA GCTTGCCAAG 
CTCAACAGCT CTTTGCAGGT GCTGAAGTTG AGGCTACCTC TGGACCCGGC AATAAGCGTC 
GAGTCCGTCG ATGTGGACAC CTGCTGCGTG TTTCCTTCCA AGACACTGCC CGTCAAGCTT 
GTCTTCCGGT CGTCCGAAGA AGCTGCCGAA AAGATTGAAA CAATCTACAA GGCTGGCGAC 
GACCTCCGTC AAGACGTTCT CATACTCACC GTGATTCGCC TCATGGACAA GCTTTGGCTG 
GAGAGCGGAA TGGACCTCAA GATGCTCACG TTCAAGTGCA CAACAACGGG AGAAAGCAGA 
GGCTTCCTCG AAGCCGTCCG GGATGTGTCG ACGCTACGGA CCATCCAGAC TAATCACTCG 
GGCGTCACGG GAGCGTTCAG CAACAGGACA ATTGCCGACT GGCTTTGGAA ACACAACTCG 
TGTAGGCCGC AGTACCAGGC GGCTGTCGAC AACTTCATCA AGTCCTGCGC CGGTTGCTGC 
GTCGCCACAT ACGTCCTGGG CGTCGGCGAC CGCCACAACG ACAACATCCT TCTGGCCACG 
TCGGGCCACA TGCTGCACAT TGATTTCTCG AAGGTCCTGG GCCATGCCGA GACGTTTGGG 
GGAATCAGGA GAGACAGGGC TCCGTTTGCA CTCACGCAAG ACATGGTCTA CGTCATCCGG 
AGGAACGGCG GAGAGGCACA GTTTGTGAGA CTCTGCTGCG AAGCCTACAA CGTCCTGCGA 
CGCAACTCCA GCCTTTTCCT CGCCCTCTTC AGCATCATGA AGCACTCGGG GCTGCCCGAC 
GTCGACCAGG ACGCGCTCGC ATACCTGCGA CGGTCCATGT CGCTGGAGGC GACGGACGAG 
CAGGCGGCGC GGCACTTTGA GAAGCGCATC CGCGAGAGCG TCATGACCCT CGCCACCAGC 
TTCAACTTCT TCATCCACAA CCTGGCGAGC CTGGGAGCCA ACACCAGGGA GTGCGCGCTG 
AGGCTCTCCT TCAACCCCCG TGACTTCAGC ATTGCTGCCG AGGGGAAGAT TGTGCAGCTG 
ACTGTGGAGG ACATCAGGCT GCGCTACCAT CCCGAGCGCT ACTACGTCTA TGTTGTCAAC 
GTGGAACGCG AGTTCCCACC GGCGCAGTCG GTCATCAGGA CCTACAAGAA CTTCCTCGAA 
CTGTACACCA AGATGAGTCG CAAGTTTCCC GCTTGCGACT TTCACCCGCT CTCGAGAGGT 
AGTGTCTTCA GCTCTGAAGG GGACGAGTCC CTTGCTCTCA AGCGCAAGCG AGACATCCAA 
CAGTTTCTGG ACACGGTGAT GGCTCAGCCG GAAGACGTGT CACACTACAG GCTCGTGTAC 
AGCTTCTTCC ATCCCCTGCC GCGAGACCTC GAACTTTTGA ACGGGGTTCC GATCACCATT 
GCCACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW004026 ( XM_029981483.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029837343.1 Ixodes scapularis phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (LOC8029418), mRNA.

Price & Availability↑

CloneID	OIa24288
Clone ID Related Accession (Same CDS sequence)	XM_029981483.1
Accession Version	XM_029981483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919717.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCACT TCTTCAAGTT TGTGCAGGCA AGGCTGGTCA AGAAACAGCC AAACCACCAC 
AGCCAGAGGC TCCTGAGGAA ACTGCAGCAT CTCGTGTGTG ACGTGCAACC AGAAGACAGT 
CCTTATTCGG GGCTTCTCAT CAGCCCTACA TTGCAAAACA ACTCGGAAGA GACATTCGAC 
GTCAAGCTCA AAATCTTCTC TGAACAACTC CACAAGCCAG TGTGTTTCTC TTGTCCGGCA 
GCTACAAGGG TGGGCTACCT TGTGGAAAGC ATCCAGCCAT TCCTGGGCAG TCCCGGAAAG 
TATGTCTTCA AGGTGACCGG CCTCGATGAG TACTTGTCCA ACGATACCCT ACTCAGCGAC 
TATGCATACA TCTGCGCCTG TAGACGTGTC GGCAAAGTGG CCTGGTTGAC TATTGTAAAT 
CTGGACAATG TGCAGAAGCC ATGGACATTA GTGACCAGCG AGAGTTGTGA TTTGGACATT 
GATGTCTCGG AGAAGATAAC GTTTGTTGCC GTCAGGGATG CCTTGGAGAC TTTCGAGTCC 
CAGGTAGAAG AGCTCATAGG CGTCGCTCTG TCGGTTGGCG GAGATCTCGT CAAGGCAGCA 
GGGGACATCA GAGCCAGTGT GACAGAACTT TGCCAGTTGT TGAACCACAC AGAGACAAGT 
GAACTGTACC AGACAACTAC AGACCTCTTA AATTTTTTCA GAGACACGCT GTCAGAGAAT 
AGAGATGGTG TCGACGCCCC CAGAAAGGTG TTCCTTGTGA CACGGTTTGT TGAAGACATT 
GAACTTGCAA TTGCAAGGTT CCTTGCAATG CATTCTCAAA GGCGTAGCGT TGAATTCACG 
GTTGAGTTTC TCGATGAGAG CTTACGACGA AGACGCACCG TAGATGCTAA CGAACTGTTC 
AGCCCCAAGG TTGAGATGCT GGTTCAGCTG CAGAAAGCTT GGGTCCAAAA CTATGACAGC 
TACAGTGTGG ACTGCGCGTT AACCTACGGC GAAAGACTCT TTAGTTATCA GACGACACGA 
CGGTTGTATG TGACGCAAAG AATTTTCCAA TGTGTAAACT TTGACGAGTA CATTTCCTTC 
AAAAACATTC AGATTCGATC CCTTCCCCGA GAAACGAGGA TGTATCTGAC GGTCGTTGGG 
CACCGAGATG GAGCTGATCA CGTCCTTGGT TGGACAGGTC TGAACATCTT TGCAGACCGA 
GAGCACTTCA ATGGAGGCGA GTTCCTCCTT GGCCTATGGT CCCCTGAAAT GCTGTCGACA 
CGCGGTCCCG TTTCCGCAAG CGAGCAGAAC AAAGGATGTT TGCTAGTCAC ACTGCCACAT 
GTCAAGAACC AGGCAGTCCT CGCCAAATCC ATCACCAGAA ACGTGCGCGA AGTTGTCAGA 
AAGCCGTTTC ATGAACTCAG TGCCGACGTG CAGCAAACTT TCCTGGACCT GACCGTAACT 
GATGTCTCCA CACCCTTGCC CACTTATGTT CGCGACCTGA TCTGGGAGCA CCGGCACCAC 
CTCTGTGACT TCTCAAGCTT GCTGCCTCAT TTGCTGAAGC TCCCGGTATG CTGGAATTCA 
GCAAACATTG AGGAGACCTA CACCCTGGTG CGGTCTTGGT GTCCACTCAG TCCGGAAGAG 
TCGCTTACGT TGCTCTCCCC CGCCTTCCCA GACAGCCTGG TCAAGAAGTA TGCGGTGCAA 
TCCCTGCAAA GCGTCGGCAG TGACGAGCTT TCCCTCTACC TTGCGCAGCT GGTCGAGTCT 
CTAAGGTTCC AAAATTGGGA CGACTCCTTC TTGGCCACGT TCCTCCTGAG GAGGGCCCGG 
CAGAGCGTCC GCCTCGCTCA TCAGCTCTTT TGGTGGCTCA CTGGCATGAT CAACGAACCG 
CTCATGAGGG ACAGGGCGCT GATCATGCGG AAGGCCCTCT TCTTTGCAGC AGGAGAGAAG 
GTCACGGCCA GCCTCCTGAC GCAGATCGTC GCAGACGAAA TGCTAGCCGA AACCGCGCGG 
AGCGTCAAGC AAGCAGCCGA CTCACAAAAA GAGGAGGTGA TGCGAAGAGA GCTTGCCAAG 
CTCAACAGCT CTTTGCAGGT GCTGAAGTTG AGGCTACCTC TGGACCCGGC AATAAGCGTC 
GAGTCCGTCG ATGTGGACAC CTGCTGCGTG TTTCCTTCCA AGACACTGCC CGTCAAGCTT 
GTCTTCCGGT CGTCCGAAGA AGCTGCCGAA AAGATTGAAA CAATCTACAA GGCTGGCGAC 
GACCTCCGTC AAGACGTTCT CATACTCACC GTGATTCGCC TCATGGACAA GCTTTGGCTG 
GAGAGCGGAA TGGACCTCAA GATGCTCACG TTCAAGTGCA CAACAACGGG AGAAAGCAGA 
GGCTTCCTCG AAGCCGTCCG GGATGTGTCG ACGCTACGGA CCATCCAGAC TAATCACTCG 
GGCGTCACGG GAGCGTTCAG CAACAGGACA ATTGCCGACT GGCTTTGGAA ACACAACTCG 
TGTAGGCCGC AGTACCAGGC GGCTGTCGAC AACTTCATCA AGTCCTGTGC CGGTTGCTGC 
GTCGCCACAT ACGTCCTGGG CGTCGGCGAC CGCCACAACG ACAACATCCT TCTGGCCACG 
TCGGGCCACA TGCTGCACAT TGATTTCTCG AAAGTCCTGG GCCATGCCGA GACGTTTGGG 
GGAATCAGGA GAGACAGGGC TCCGTTTGCA CTCACGCAAG ACATGGTCTA CGTCATCCGG 
AGGAACGGCG GAGAGGCACA GTTTGTGAGA CTCTGCTGCG AAGCCTACAA CGTCCTGCGA 
CGCAACTCCA GCCTTTTCCT CGCCCTCTTC AGCATCATGA AGCACTCGGG GCTGCCCGAC 
GTCGACCAGG ACGCGCTCGC ATACCTGCGA CGGTCCATGT CGCTGGAGGC GACGGACGAG 
CAGGCGGCGC GGCACTTTGA GAAGCGCATC CGCGAGAGCG TCATGACCCT CGCCACCAGC 
TTCAACTTCT TCATCCACAA CCTGGCGAGC CTGGGAGCCA ATACCAGGGA GTGCGCGCTG 
AGGCTCTCCT TCAACCCCCG TGACTTCAGC ATTGCTGCCG AGGGGAAGAT TGTGCAGCTG 
ACTGTGGAGG ACATCAGGCT GCGCTACCAT CCCGAGCGTT ACTACGTCTA TGTTGTCAAC 
GTGGAACGCG AGTTCCCACC GGCGCAGTCG GTCATCAGGA CCTACAAGAA CTTCCTCGAA 
CTGTACACCA AGATGAGTCG CAAGTTTCCC GCTTGCGACT TTCACCCGCT CTCGAGAGGT 
AGTGTCTTCA GCACTGAAGG GGACGAGTCC CTTGCTCTCA AGCGCAAGCG AGACATCCAA 
CAGTTTCTGG ACACGGTGAT GGCTCAGCCG GAAGACGTGT CACACTACAG GCTCGTGTAC 
AGCTTCTTCC ATCCCCTGCC GCGAGACCTC GAGCTTTTGA ACGGGGTTCC GATCACCATT 
GCCACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029837343.1
CDS	390..3818
Translation	MSHFFKFVQARLVKKQPNHHSQRLLRKLQHLVCDVQPEDSPYSGLLISPTLQNNSEETFDVKLKIFSEQLHKPVCFSCPAATRVGYLVESIQPFLGSPGKYVFKVTGLDEYLSNDTLLSDYAYICACRRVGKVAWLTIVNLDNVQKPWTLVTSESCDLDIDVSEKITFVAVRDALETFESQVEELIGVALSVGGDLVKAAGDIRASVTELCQLLNHTETSELYQTTTDLLNFFRDTLSENRDGVDAPRKVFLVTRFVEDIELAIARFLAMHSQRRSVEFTVEFLDESLRRRRTVDANELFSPKVEMLVQLQKAWVQNYDSYSVDCALTYGERLFSYQTTRRLYVTQRIFQCVNFDEYISFKNIQIRSLPRETRMYLTVVGHRDGADHVLGWTGLNIFADREHFNGGEFLLGLWSPEMLSTRGPVSASEQNKGCLLVTLPHVKNQAVLAKSITRNVREVVRKPFHELSADVQQTFLDLTVTDVSTPLPTYVRDLIWEHRHHLCDFSSLLPHLLKLPVCWNSANIEETYTLVRSWCPLSPEESLTLLSPAFPDSLVKKYAVQSLQSVGSDELSLYLAQLVESLRFQNWDDSFLATFLLRRARQSVRLAHQLFWWLTGMINEPLMRDRALIMRKALFFAAGEKVTASLLTQIVADEMLAETARSVKQAADSQKEEVMRRELAKLNSSLQVLKLRLPLDPAISVESVDVDTCCVFPSKTLPVKLVFRSSEEAAEKIETIYKAGDDLRQDVLILTVIRLMDKLWLESGMDLKMLTFKCTTTGESRGFLEAVRDVSTLRTIQTNHSGVTGAFSNRTIADWLWKHNSCRPQYQAAVDNFIKSCAGCCVATYVLGVGDRHNDNILLATSGHMLHIDFSKVLGHAETFGGIRRDRAPFALTQDMVYVIRRNGGEAQFVRLCCEAYNVLRRNSSLFLALFSIMKHSGLPDVDQDALAYLRRSMSLEATDEQAARHFEKRIRESVMTLATSFNFFIHNLASLGANTRECALRLSFNPRDFSIAAEGKIVQLTVEDIRLRYHPERYYVYVVNVEREFPPAQSVIRTYKNFLELYTKMSRKFPACDFHPLSRGSVFSTEGDESLALKRKRDIQQFLDTVMAQPEDVSHYRLVYSFFHPLPRDLELLNGVPITIAT

Target ORF information:

RefSeq Version	XM_029981483.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (LOC8029418), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029981483.1

ORF Insert Sequence:

ATGTCCCACT TCTTCAAGTT TGTGCAGGCA AGGCTGGTCA AGAAACAGCC AAACCACCAC 
AGCCAGAGGC TCCTGAGGAA ACTGCAGCAT CTCGTGTGTG ACGTGCAACC AGAAGACAGT 
CCTTATTCGG GGCTTCTCAT CAGCCCTACA TTGCAAAACA ACTCGGAAGA GACATTCGAC 
GTCAAGCTCA AAATCTTCTC TGAACAACTC CACAAGCCAG TGTGTTTCTC TTGTCCGGCA 
GCTACAAGGG TGGGCTACCT TGTGGAAAGC ATCCAGCCAT TCCTGGGCAG TCCCGGAAAG 
TATGTCTTCA AGGTGACCGG CCTCGATGAG TACTTGTCCA ACGATACCCT ACTCAGCGAC 
TATGCATACA TCTGCGCCTG TAGACGTGTC GGCAAAGTGG CCTGGTTGAC TATTGTAAAT 
CTGGACAATG TGCAGAAGCC ATGGACATTA GTGACCAGCG AGAGTTGTGA TTTGGACATT 
GATGTCTCGG AGAAGATAAC GTTTGTTGCC GTCAGGGATG CCTTGGAGAC TTTCGAGTCC 
CAGGTAGAAG AGCTCATAGG CGTCGCTCTG TCGGTTGGCG GAGATCTCGT CAAGGCAGCA 
GGGGACATCA GAGCCAGTGT GACAGAACTT TGCCAGTTGT TGAACCACAC AGAGACAAGT 
GAACTGTACC AGACAACTAC AGACCTCTTA AATTTTTTCA GAGACACGCT GTCAGAGAAT 
AGAGATGGTG TCGACGCCCC CAGAAAGGTG TTCCTTGTGA CACGGTTTGT TGAAGACATT 
GAACTTGCAA TTGCAAGGTT CCTTGCAATG CATTCTCAAA GGCGTAGCGT TGAATTCACG 
GTTGAGTTTC TCGATGAGAG CTTACGACGA AGACGCACCG TAGATGCTAA CGAACTGTTC 
AGCCCCAAGG TTGAGATGCT GGTTCAGCTG CAGAAAGCTT GGGTCCAAAA CTATGACAGC 
TACAGTGTGG ACTGCGCGTT AACCTACGGC GAAAGACTCT TTAGTTATCA GACGACACGA 
CGGTTGTATG TGACGCAAAG AATTTTCCAA TGTGTAAACT TTGACGAGTA CATTTCCTTC 
AAAAACATTC AGATTCGATC CCTTCCCCGA GAAACGAGGA TGTATCTGAC GGTCGTTGGG 
CACCGAGATG GAGCTGATCA CGTCCTTGGT TGGACAGGTC TGAACATCTT TGCAGACCGA 
GAGCACTTCA ATGGAGGCGA GTTCCTCCTT GGCCTATGGT CCCCTGAAAT GCTGTCGACA 
CGCGGTCCCG TTTCCGCAAG CGAGCAGAAC AAAGGATGTT TGCTAGTCAC ACTGCCACAT 
GTCAAGAACC AGGCAGTCCT CGCCAAATCC ATCACCAGAA ACGTGCGCGA AGTTGTCAGA 
AAGCCGTTTC ATGAACTCAG TGCCGACGTG CAGCAAACTT TCCTGGACCT GACCGTAACT 
GATGTCTCCA CACCCTTGCC CACTTATGTT CGCGACCTGA TCTGGGAGCA CCGGCACCAC 
CTCTGTGACT TCTCAAGCTT GCTGCCTCAT TTGCTGAAGC TCCCGGTATG CTGGAATTCA 
GCAAACATTG AGGAGACCTA CACCCTGGTG CGGTCTTGGT GTCCACTCAG TCCGGAAGAG 
TCGCTTACGT TGCTCTCCCC CGCCTTCCCA GACAGCCTGG TCAAGAAGTA TGCGGTGCAA 
TCCCTGCAAA GCGTCGGCAG TGACGAGCTT TCCCTCTACC TTGCGCAGCT GGTCGAGTCT 
CTAAGGTTCC AAAATTGGGA CGACTCCTTC TTGGCCACGT TCCTCCTGAG GAGGGCCCGG 
CAGAGCGTCC GCCTCGCTCA TCAGCTCTTT TGGTGGCTCA CTGGCATGAT CAACGAACCG 
CTCATGAGGG ACAGGGCGCT GATCATGCGG AAGGCCCTCT TCTTTGCAGC AGGAGAGAAG 
GTCACGGCCA GCCTCCTGAC GCAGATCGTC GCAGACGAAA TGCTAGCCGA AACCGCGCGG 
AGCGTCAAGC AAGCAGCCGA CTCACAAAAA GAGGAGGTGA TGCGAAGAGA GCTTGCCAAG 
CTCAACAGCT CTTTGCAGGT GCTGAAGTTG AGGCTACCTC TGGACCCGGC AATAAGCGTC 
GAGTCCGTCG ATGTGGACAC CTGCTGCGTG TTTCCTTCCA AGACACTGCC CGTCAAGCTT 
GTCTTCCGGT CGTCCGAAGA AGCTGCCGAA AAGATTGAAA CAATCTACAA GGCTGGCGAC 
GACCTCCGTC AAGACGTTCT CATACTCACC GTGATTCGCC TCATGGACAA GCTTTGGCTG 
GAGAGCGGAA TGGACCTCAA GATGCTCACG TTCAAGTGCA CAACAACGGG AGAAAGCAGA 
GGCTTCCTCG AAGCCGTCCG GGATGTGTCG ACGCTACGGA CCATCCAGAC TAATCACTCG 
GGCGTCACGG GAGCGTTCAG CAACAGGACA ATTGCCGACT GGCTTTGGAA ACACAACTCG 
TGTAGGCCGC AGTACCAGGC GGCTGTCGAC AACTTCATCA AGTCCTGTGC CGGTTGCTGC 
GTCGCCACAT ACGTCCTGGG CGTCGGCGAC CGCCACAACG ACAACATCCT TCTGGCCACG 
TCGGGCCACA TGCTGCACAT TGATTTCTCG AAAGTCCTGG GCCATGCCGA GACGTTTGGG 
GGAATCAGGA GAGACAGGGC TCCGTTTGCA CTCACGCAAG ACATGGTCTA CGTCATCCGG 
AGGAACGGCG GAGAGGCACA GTTTGTGAGA CTCTGCTGCG AAGCCTACAA CGTCCTGCGA 
CGCAACTCCA GCCTTTTCCT CGCCCTCTTC AGCATCATGA AGCACTCGGG GCTGCCCGAC 
GTCGACCAGG ACGCGCTCGC ATACCTGCGA CGGTCCATGT CGCTGGAGGC GACGGACGAG 
CAGGCGGCGC GGCACTTTGA GAAGCGCATC CGCGAGAGCG TCATGACCCT CGCCACCAGC 
TTCAACTTCT TCATCCACAA CCTGGCGAGC CTGGGAGCCA ATACCAGGGA GTGCGCGCTG 
AGGCTCTCCT TCAACCCCCG TGACTTCAGC ATTGCTGCCG AGGGGAAGAT TGTGCAGCTG 
ACTGTGGAGG ACATCAGGCT GCGCTACCAT CCCGAGCGTT ACTACGTCTA TGTTGTCAAC 
GTGGAACGCG AGTTCCCACC GGCGCAGTCG GTCATCAGGA CCTACAAGAA CTTCCTCGAA 
CTGTACACCA AGATGAGTCG CAAGTTTCCC GCTTGCGACT TTCACCCGCT CTCGAGAGGT 
AGTGTCTTCA GCACTGAAGG GGACGAGTCC CTTGCTCTCA AGCGCAAGCG AGACATCCAA 
CAGTTTCTGG ACACGGTGAT GGCTCAGCCG GAAGACGTGT CACACTACAG GCTCGTGTAC 
AGCTTCTTCC ATCCCCTGCC GCGAGACCTC GAGCTTTTGA ACGGGGTTCC GATCACCATT 
GCCACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IscW_ISCW004026 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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