LOC8024169 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8024169
Entrez Gene ID	8024169
Full Name	ubiquitin carboxyl-terminal hydrolase, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029979015.1	XP_029834875.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
XM_002404537.1	XP_002404581.1	ubiquitin carboxyl-terminal hydrolase, putative
XM_029979016.1	XP_029834876.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
XM_029979017.1	XP_029834877.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8024169 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8024169 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa00609	XM_002404537.1 Latest version!	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OIa22177	XM_029979015.1 Latest version!	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OIa22177	XM_029979016.1 Latest version!	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OIa22178	XM_029979017.1 Latest version!	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8024169 ( XM_002404537.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002404581.1 Ixodes scapularis ubiquitin carboxyl-terminal hydrolase, putative, mRNA.

Price & Availability↑

CloneID	OIa00609
Clone ID Related Accession (Same CDS sequence)	XM_002404537.1
Accession Version	XM_002404537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	ubiquitin carboxyl-terminal hydrolase, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002523594). COMPLETENESS: incomplete on both ends.

ATGCGCTCCT TGCGGGATCC CGACTACCCA AGATGCCCCG CGCGCGAATC CACCATAGCC 
GCGGCCATCT TGTTTATTTT CATGCTCCGT GCCGCCGCGA TCGTGAGTCT CGGCGTCCCC 
TGCAAAGTCC GTTTTGTCGT CGCATGGTTT CCCCTGTCCG GGGGGCTGAC ACCAGCAGAC 
ATTGGGCGAA TCGCGAGCAA GCACGTCAAA GTGACAGTGT CCGGCAAAAA CCCCCTGGCC 
GCAGGCAAAG AACGCTCCAA CAGCTCTGCC CGATCTAAGC GCAACGCCGC CACCCGTGAC 
ACCACAGCTG CCGTCGGACT TATAATGAAC CACGCTGTTC TTCATGACAA GGACAGCGGT 
CAGGCAGACT TGGAGGAAAT GGATACACAA GAAGGTGACG AAAACGTGGC CCCTCCACCG 
TCGCAGCAGC AGCAGCAGCC GCCACAGCCG ACCCAGCAGG CCCAGCCCCT GCACCACCCG 
CCGCCCCCTC CCCCGCCCGA GACGGGCGGG CGGGAGCGCT TTGCCAACGG CGTGTCCCCC 
GCGGTGCCGG CGGCAGCGGC GGCGCCAGAT GGCCTCGATG AGCCCATGGA GGAGGAGGAC 
GAGGCCCGGT CAGAGGCCAC CTTCCGCTTT GTGGTGACGG GCTTCTCGAG GCTGCGGGAG 
TCAGTGCTCA GTCCGGCCAC CCAAGTGCGC AACCTCCCCT GGAAAATCAT GGTGATGCCG 
CGCACGAATG CGGGCAACGA CCGGACGCCA ACCAAAAGTC TGGGCTTCTT CCTACAATGC 
AACGGCGAGT CGGAGTCCTC GACATGGTCA TGCAACGCGA CGGCAGAGCT TCGCATCATC 
AGCCAGAAGG AGGGCGTCGA CAACTTCGTA AGAAAAATCC AGCACCTATT CTATAGCAAG 
GAGAACGACT GGGGATTCAG TCACTTCATG ACGTGGACGG ACGTTCTCGA TCCGGAGAAA 
GGGTTCATTC TGGACGACAC CATCATCCTG GAGGTGTGGG TGTCGGCGGA CGCACCTCAC 
GGGGTCAGCT GGGACTCCAA AAAGCACACG GGCTACGTGG GCCTGAAGAA CCAGGGTGCC 
ACGTGCTACA TGAACTCCCT CCTGCAGACA CTCTTCTTCA CGAATCAGCT CCGCAAGGCG 
GTGTACCAGA TGCCGACGGA GAGCGACGAC TCGTCCAAGA GCGTGGCGCT GGCGCTACAG 
CGGGTCTTCT ACGAGCTGCA GTTCTCGGAC AAGCCCGTGG GCACCAAGAA GCTCACGAAG 
TCCTTCGGCT GGGAGACGCT CGACTCTTTT ATGCAGCACG ACGTCCAGGA GCTGTGTAGA 
GTGCTGCTGG ACAACATGGA GAGCAAGATG AAGGGCACCT GCGTGGAGGG CACCATCCCC 
CGGCTCTTTG AGGGCAAGAT GATCTCGTTC ATCAAGTGCA AGCACGTGGA GTACACCTCC 
AGGCGCATGG AGCCCTTCTA CGACATCCAG CTCAATGTCA AGGGCAAGAA GAACATCCAC 
GAGTCCTTCC AGGACTACTG CGCCACGGAG AGCCTGGACG GGGAGAACAA GTACGACGCA 
GGGGAGTACG GGCTGCAGGA GGCGGAGAAA GGCATCATTT TCGCCTGCCT GCCCCCCGTG 
GTGCACCTGC ACCTGCTGCG CTTCCAGTAC GACCCCCTCA CGGACAACAA CGTCAAGCTC 
AACGACCGCT TTGAAGACGT CGTCGCGGCG TTTTCCAATG CGTTGCCGAC CGAGGCACCC 
TCTACGTTCC CCCAGGGCCC GAGCCCGGCG ACGTACACCC TGCACGCGGT GCTGGTGCAC 
TCCGGGGACA ACCACGGGGG CCACTATGTG GTGTTCATCA ACCCCCGGGG GGACGGGCGC 
TGGTGCAAGT TCGACGACGA CGTGGTTTCG CGCTGCACCA AGCAGGAGGC CATCGACCAC 
AACTTCGGGG GCACGGAGGA CGACGTGGCC GTCAGCCGCC ACTGCACCAA TGCCTACATG 
CTGGTCTACA TCAGGGACAG CGCCATCATT CTTACGGTTG CCACTGTCGG CTGCGAGTCT 
TGGATTAGTC CCGATTTTCG CTTGGCTATT ATATGGCGCC AGGAGGCCCT GCGGCGCAAG 
GAGCGCAACG AGGCGCACCT GTACATGAGC GTGCAGGTGC TGACGGAGGA CAGCTTTGCG 
GGCCACCAGG GCAACGACCT GTACGACCCG GAGAAGGCCT CTTACCGCTC CTTCAAGGTG 
AAGAAGCAAG CCACCCTGCG AGACCTGCTG GACCTGATTG CGGACCAGAT GCGCTTCCCC 
CTGCACGCCA TCCGGCCCTG GTCCATCACC TACCGCTCCA ACCAAACCTT CCGGCCGGCC 
GCCGTGGACC TGGACGCGGA CCTCAACAAG ACGGTGATTG ACCTGTCCGA GAACGCCAAC 
CCCTGGACGA TCTTCCTGGA GACGATCGTG CCGGACCAGC CGATGGACCG GTTGCCGGAG 
TTCGACAAAG AGACGGACGT GCTGCTCTTC TTCAAGATGT ACGACCCGAG GCTGAAACAG 
ATCGCCTACT GCGGCCACAC CTACATGCCC ATCACCGTCA AGGCCAAGGA GCTGGTGCCG 
CTGCTCAACA AGAGGGCGGG GTTTCCCCCT GACACGGAGC TGGTGCTCTA CGAGGAGGTG 
AAGCCAAACA TTGTGGACCG CATTGAGGAC CTGGAGCTGC CCCTGGAGAA GGTCCTGGAG 
GAGCTGATGG ACGGAGACAT AATCGTCTTT CAGAAGCTGG ACCACAATGC GGACGAGTAC 
GAGCTGCCCA CCGTCAAGGA CTACTTCAGA GACCTCTTCT ACAGGGTGGA GGTGACCTTC 
TGTGATAAAT CTATTGCCAA TGACCCCGGC TTCACGATGG AGCTTTCTCT CAAGATGAAC 
TATGACCAGA TCGCCAGGGC GGTGGCGCAA CGGCTAGGCA CAGACCCTTA TTTATTACAA 
TTTTTCAAAG TTCAGAGTTA CCGAGAGGGC CCCGGTAACC CTCTGAGGTG TACATACGAG 
GGGACGCTCA AGGACCTCCT GGTATTCTTC AAGCCCAGGC AGCCCAAGAA GATCTATTAC 
CAGCAGCTGA GCATACGGAT AAACGAGCTA GAAAGCAAGA AGCAGTTCAA GTGCGTCTGG 
GTCAACAGCA AGTTCAAGGA AGAGCGGGAA CCCTTCACGA AGGTCAAGGA CAGGATTCAG 
AAGAAGCTGG ACATCCCTGA CAAGGAGTTC GAAAAGTACA AGTTCGCCAT TGTCGTAATG 
GGGCGGGCAC AGTTCATCGA GGATGCAGAC TACGTGGTGA ACCTGCAAGA CTTCATGGCG 
CAACCTCCTC AAGGTGCGGC GATGCACCCC CGTCCCTGGC TGGGCATCGA ACACATCAAC 
AAGGCACCCA AGAGGTCCCG CTACAACTAC CTGGAGAAGG CCATCAAGAT CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002404581.1
CDS	1..3420
Translation	MRSLRDPDYPRCPARESTIAAAILFIFMLRAAAIVSLGVPCKVRFVVAWFPLSGGLTPADIGRIASKHVKVTVSGKNPLAAGKERSNSSARSKRNAATRDTTAAVGLIMNHAVLHDKDSGQADLEEMDTQEGDENVAPPPSQQQQQPPQPTQQAQPLHHPPPPPPPETGGRERFANGVSPAVPAAAAAPDGLDEPMEEEDEARSEATFRFVVTGFSRLRESVLSPATQVRNLPWKIMVMPRTNAGNDRTPTKSLGFFLQCNGESESSTWSCNATAELRIISQKEGVDNFVRKIQHLFYSKENDWGFSHFMTWTDVLDPEKGFILDDTIILEVWVSADAPHGVSWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYQMPTESDDSSKSVALALQRVFYELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNMESKMKGTCVEGTIPRLFEGKMISFIKCKHVEYTSRRMEPFYDIQLNVKGKKNIHESFQDYCATESLDGENKYDAGEYGLQEAEKGIIFACLPPVVHLHLLRFQYDPLTDNNVKLNDRFEDVVAAFSNALPTEAPSTFPQGPSPATYTLHAVLVHSGDNHGGHYVVFINPRGDGRWCKFDDDVVSRCTKQEAIDHNFGGTEDDVAVSRHCTNAYMLVYIRDSAIILTVATVGCESWISPDFRLAIIWRQEALRRKERNEAHLYMSVQVLTEDSFAGHQGNDLYDPEKASYRSFKVKKQATLRDLLDLIADQMRFPLHAIRPWSITYRSNQTFRPAAVDLDADLNKTVIDLSENANPWTIFLETIVPDQPMDRLPEFDKETDVLLFFKMYDPRLKQIAYCGHTYMPITVKAKELVPLLNKRAGFPPDTELVLYEEVKPNIVDRIEDLELPLEKVLEELMDGDIIVFQKLDHNADEYELPTVKDYFRDLFYRVEVTFCDKSIANDPGFTMELSLKMNYDQIARAVAQRLGTDPYLLQFFKVQSYREGPGNPLRCTYEGTLKDLLVFFKPRQPKKIYYQQLSIRINELESKKQFKCVWVNSKFKEEREPFTKVKDRIQKKLDIPDKEFEKYKFAIVVMGRAQFIEDADYVVNLQDFMAQPPQGAAMHPRPWLGIEHINKAPKRSRYNYLEKAIKIHN

Target ORF information:

RefSeq Version	XM_002404537.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002404537.1

ORF Insert Sequence:

ATGCGCTCCT TGCGGGATCC CGACTACCCA AGATGCCCCG CGCGCGAATC CACCATAGCC 
GCGGCCATCT TGTTTATTTT CATGCTCCGT GCCGCCGCGA TCGTGAGTCT CGGCGTCCCC 
TGCAAAGTCC GTTTTGTCGT CGCATGGTTT CCCCTGTCCG GGGGGCTGAC ACCAGCAGAC 
ATTGGGCGAA TCGCGAGCAA GCACGTCAAA GTGACAGTGT CCGGCAAAAA CCCCCTGGCC 
GCAGGCAAAG AACGCTCCAA CAGCTCTGCC CGATCTAAGC GCAACGCCGC CACCCGTGAC 
ACCACAGCTG CCGTCGGACT TATAATGAAC CACGCTGTTC TTCATGACAA GGACAGCGGT 
CAGGCAGACT TGGAGGAAAT GGATACACAA GAAGGTGACG AAAACGTGGC CCCTCCACCG 
TCGCAGCAGC AGCAGCAGCC GCCACAGCCG ACCCAGCAGG CCCAGCCCCT GCACCACCCG 
CCGCCCCCTC CCCCGCCCGA GACGGGCGGG CGGGAGCGCT TTGCCAACGG CGTGTCCCCC 
GCGGTGCCGG CGGCAGCGGC GGCGCCAGAT GGCCTCGATG AGCCCATGGA GGAGGAGGAC 
GAGGCCCGGT CAGAGGCCAC CTTCCGCTTT GTGGTGACGG GCTTCTCGAG GCTGCGGGAG 
TCAGTGCTCA GTCCGGCCAC CCAAGTGCGC AACCTCCCCT GGAAAATCAT GGTGATGCCG 
CGCACGAATG CGGGCAACGA CCGGACGCCA ACCAAAAGTC TGGGCTTCTT CCTACAATGC 
AACGGCGAGT CGGAGTCCTC GACATGGTCA TGCAACGCGA CGGCAGAGCT TCGCATCATC 
AGCCAGAAGG AGGGCGTCGA CAACTTCGTA AGAAAAATCC AGCACCTATT CTATAGCAAG 
GAGAACGACT GGGGATTCAG TCACTTCATG ACGTGGACGG ACGTTCTCGA TCCGGAGAAA 
GGGTTCATTC TGGACGACAC CATCATCCTG GAGGTGTGGG TGTCGGCGGA CGCACCTCAC 
GGGGTCAGCT GGGACTCCAA AAAGCACACG GGCTACGTGG GCCTGAAGAA CCAGGGTGCC 
ACGTGCTACA TGAACTCCCT CCTGCAGACA CTCTTCTTCA CGAATCAGCT CCGCAAGGCG 
GTGTACCAGA TGCCGACGGA GAGCGACGAC TCGTCCAAGA GCGTGGCGCT GGCGCTACAG 
CGGGTCTTCT ACGAGCTGCA GTTCTCGGAC AAGCCCGTGG GCACCAAGAA GCTCACGAAG 
TCCTTCGGCT GGGAGACGCT CGACTCTTTT ATGCAGCACG ACGTCCAGGA GCTGTGTAGA 
GTGCTGCTGG ACAACATGGA GAGCAAGATG AAGGGCACCT GCGTGGAGGG CACCATCCCC 
CGGCTCTTTG AGGGCAAGAT GATCTCGTTC ATCAAGTGCA AGCACGTGGA GTACACCTCC 
AGGCGCATGG AGCCCTTCTA CGACATCCAG CTCAATGTCA AGGGCAAGAA GAACATCCAC 
GAGTCCTTCC AGGACTACTG CGCCACGGAG AGCCTGGACG GGGAGAACAA GTACGACGCA 
GGGGAGTACG GGCTGCAGGA GGCGGAGAAA GGCATCATTT TCGCCTGCCT GCCCCCCGTG 
GTGCACCTGC ACCTGCTGCG CTTCCAGTAC GACCCCCTCA CGGACAACAA CGTCAAGCTC 
AACGACCGCT TTGAAGACGT CGTCGCGGCG TTTTCCAATG CGTTGCCGAC CGAGGCACCC 
TCTACGTTCC CCCAGGGCCC GAGCCCGGCG ACGTACACCC TGCACGCGGT GCTGGTGCAC 
TCCGGGGACA ACCACGGGGG CCACTATGTG GTGTTCATCA ACCCCCGGGG GGACGGGCGC 
TGGTGCAAGT TCGACGACGA CGTGGTTTCG CGCTGCACCA AGCAGGAGGC CATCGACCAC 
AACTTCGGGG GCACGGAGGA CGACGTGGCC GTCAGCCGCC ACTGCACCAA TGCCTACATG 
CTGGTCTACA TCAGGGACAG CGCCATCATT CTTACGGTTG CCACTGTCGG CTGCGAGTCT 
TGGATTAGTC CCGATTTTCG CTTGGCTATT ATATGGCGCC AGGAGGCCCT GCGGCGCAAG 
GAGCGCAACG AGGCGCACCT GTACATGAGC GTGCAGGTGC TGACGGAGGA CAGCTTTGCG 
GGCCACCAGG GCAACGACCT GTACGACCCG GAGAAGGCCT CTTACCGCTC CTTCAAGGTG 
AAGAAGCAAG CCACCCTGCG AGACCTGCTG GACCTGATTG CGGACCAGAT GCGCTTCCCC 
CTGCACGCCA TCCGGCCCTG GTCCATCACC TACCGCTCCA ACCAAACCTT CCGGCCGGCC 
GCCGTGGACC TGGACGCGGA CCTCAACAAG ACGGTGATTG ACCTGTCCGA GAACGCCAAC 
CCCTGGACGA TCTTCCTGGA GACGATCGTG CCGGACCAGC CGATGGACCG GTTGCCGGAG 
TTCGACAAAG AGACGGACGT GCTGCTCTTC TTCAAGATGT ACGACCCGAG GCTGAAACAG 
ATCGCCTACT GCGGCCACAC CTACATGCCC ATCACCGTCA AGGCCAAGGA GCTGGTGCCG 
CTGCTCAACA AGAGGGCGGG GTTTCCCCCT GACACGGAGC TGGTGCTCTA CGAGGAGGTG 
AAGCCAAACA TTGTGGACCG CATTGAGGAC CTGGAGCTGC CCCTGGAGAA GGTCCTGGAG 
GAGCTGATGG ACGGAGACAT AATCGTCTTT CAGAAGCTGG ACCACAATGC GGACGAGTAC 
GAGCTGCCCA CCGTCAAGGA CTACTTCAGA GACCTCTTCT ACAGGGTGGA GGTGACCTTC 
TGTGATAAAT CTATTGCCAA TGACCCCGGC TTCACGATGG AGCTTTCTCT CAAGATGAAC 
TATGACCAGA TCGCCAGGGC GGTGGCGCAA CGGCTAGGCA CAGACCCTTA TTTATTACAA 
TTTTTCAAAG TTCAGAGTTA CCGAGAGGGC CCCGGTAACC CTCTGAGGTG TACATACGAG 
GGGACGCTCA AGGACCTCCT GGTATTCTTC AAGCCCAGGC AGCCCAAGAA GATCTATTAC 
CAGCAGCTGA GCATACGGAT AAACGAGCTA GAAAGCAAGA AGCAGTTCAA GTGCGTCTGG 
GTCAACAGCA AGTTCAAGGA AGAGCGGGAA CCCTTCACGA AGGTCAAGGA CAGGATTCAG 
AAGAAGCTGG ACATCCCTGA CAAGGAGTTC GAAAAGTACA AGTTCGCCAT TGTCGTAATG 
GGGCGGGCAC AGTTCATCGA GGATGCAGAC TACGTGGTGA ACCTGCAAGA CTTCATGGCG 
CAACCTCCTC AAGGTGCGGC GATGCACCCC CGTCCCTGGC TGGGCATCGA ACACATCAAC 
AAGGCACCCA AGAGGTCCCG CTACAACTAC CTGGAGAAGG CCATCAAGAT CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8024169 ( XM_029979015.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029834875.1 Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa22177
Clone ID Related Accession (Same CDS sequence)	XM_029979015.1 , XM_029979016.1
Accession Version	XM_029979015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGTG CCGCCGCGAT CGTGAGTCTC GGCGTCCCCT GCAAAGTCCG TTTTGTCGTC 
GCATGGTTTC CCCTGTCCGG GGGGCTGACA CCAGCAGACA TTGGGCGAAT CGCGAGCAAG 
CACGTCAAAG TGACAGTGTC CGGCAAAAAC CCCCTGGCCG CAGGCAAAGA ACGCTCCAAC 
AGCTCTGCCC GATCTAAGCG CAACGCCGCC ACCCGTGACA CCACAGCTGC CGTCGGACTT 
ATAATGAACC ACGCTGTTCT TCATGACAAG GACAGCGGTC AGGCAGACTT GGAGGAAATG 
GATACACAAG AAGGTGACGA AAACGTGGCC CCTCCACCGT CGCAGCAGCA GCAGCAGCCG 
CCACAGCCGA CCCAGCAGGC CCAGCCCCTG CACCACCCGC CGCCCCCTCC CCCGCCTGAG 
ACGGGCGGGC GGGAGCGCTT TGCCAACGGC GTGTCCCCCG CGGTGGGCGA CGTGCACAGC 
CCGCCCCCTC CCGCTCCTCC CGCCTGCGAC GAGCCCATGG CGCCGGCCCC GCCCCCCGCC 
GCCGACGCAG GCGCGCTGAC GAAGGTGCCG GCGGCAGCAG CGGCGCCGGA TGGCCTCGAC 
GAGCCCATGG AGGAGGAGGA CGAGGCCCGG TCGGAGGCCA CCTTCCGCTT TGTGGTGACG 
GGCTTCTCGA GGCTGCGGGA GTCGGTGCTC AGTCCGGCCA CCCAAGTGCG CAACCTCCCC 
TGGAAAATCA TGGTGATGCC GCGCACGAAT GCGGGCAACG ACCGGACACC GACCAAGAGT 
CTGGGCTTCT TCCTACAATG CAACGGCGAG TCGGAGTCCT CGACGTGGTC ATGCAACGCG 
ACGGCGGAGC TTCGCATCAT CAGCCAGAAG GAGGGCGTCG ACAACTTCGT AAGAAAAATC 
CAGCACCTGT TCTATAGCAA GGAGAACGAC TGGGGATTCA GTCACTTCAT GACGTGGACG 
GACGTTCTCG ATCCGGAGAA AGGGTTCATT CTGGACGACA CCATCATCCT GGAGGTGTGG 
GTGTCGGCGG ACGCGCCTCA CGGGGTCAGC TGGGACTCCA AAAAGCACAC GGGCTACGTG 
GGCCTGAAGA ACCAGGGTGC CACGTGCTAC ATGAACTCCC TCCTGCAGAC ACTCTTCTTC 
ACGAATCAGC TCCGCAAGGC GGTGTACCAG ATGCCGACGG AGAGCGACGA CTCGTCCAAG 
AGCGTGGCGC TGGCGCTACA GCGGGTCTTC TACGAGCTGC AGTTCTCGGA CAAGCCCGTG 
GGCACCAAGA AGCTCACGAA GTCCTTCGGC TGGGAGACGC TCGACTCTTT TATGCAGCAC 
GACGTCCAGG AGCTGTGTAG AGTGCTGCTG GACAACATGG AGAGCAAGAT GAAGGGCACC 
TGCGTGGAGG GCACCATCCC CCGGCTCTTT GAGGGCAAGA TGATCTCGTT CATCAAGTGC 
AAGCACGTGG AGTACACCTC CAGGCGCATG GAGCCCTTCT ACGACATCCA GCTGAACGTC 
AAGGGCAAGA AGAACATCCA CGAGTCCTTC CAGGACTACT GCGCCACAGA GAGCCTGGAC 
GGGGAGAACA AGTACGACGC AGGGGAGTAC GGGCTGCAGG AGGCGGAGAA AGGCATCATT 
TTCGCCTGCC TGCCCCCCGT GGTGCACCTG CACCTGCTGC GCTTCCAGTA CGACCCCCTC 
ACGGACAACA ACGTCAAGCT CAACGACCGC TTTGAGTTTC CCGAGAAGCT CAACCTCTCA 
GACTTCCTCC ACGAGAAGGG CCCGAGCCCG GCGACGTACA CCCTGCACGC GGTGCTGGTG 
CACTCCGGGG ACAACCACGG GGGCCACTAC GTGGTGTTCA TCAACCCCCG GGGGGACGGG 
CGCTGGTGCA AGTTCGACGA CGACGTGGTC TCGCGCTGCA CCAAGCAGGA GGCCATCGAC 
CACAACTTCG GGGGCACGGA GGACGACGTG GCCGTCAGCC GCCACTGCAC CAATGCCTAC 
ATGCTGGTGT ACATCAGGGA CAGCGCCATC AACGAGGTCC TGCAGTCGGT GACGGAAAAC 
GACATCCCCG AGCAGCTGGT CGAGAGGCTT CAGGAGGAAA AGCGCCAGGA GGCCCTGCGG 
CGCAAGGAGC GCAACGAGGC GCACCTGTAC ATGAGCGTGC AGGTGCTGAC GGAGGATAGC 
TTTGCGGGAC ACCAGGGCAA CGACCTGTAC GACCCGGAGA AGGCCTCTTA CCGCTCCTTC 
AAGGTGAAGA AGCAAGCCAC CCTGCGGGAC CTGCTGGACC TGATTGCAGA CCAGATGCGC 
TTCCCCCTGC ACGCCATCCG GCCCTGGTCC ATCACCTACC GCTCCAACCA AACCTTCCGG 
CCGGCCGCCG TGGACCTTGA CGCGGACCTC AACAAGACGG TGATTGACCT GTCCGAGAAT 
GCCAACCCCT GGACGATCTT CCTGGAGACG ATCGTGCCGG ACCAGCCGAT GGACCGGTTG 
CCGGAGTTCG ACAAAGAGAC GGACGTGCTG CTCTTCTTCA AGATGTACGA CCCAAGGCTG 
AAGCAGATCG CCTACTGCGG CCACACCTAC ATGCCCATCA CCGTCAAGGC CAAGGAGCTG 
GTGCCGCTGC TCAACAAGAG GGCGGGGTTT CCTCCTGACA CGGAGCTGGT GCTCTACGAG 
GAGGTGAAGC CAAACATTGT GGACCGCATT GAGGACCTGG AGCTGCCCCT GGAGAAGGTC 
CTGGAGGAGC TGATGGACGG AGACATAATC GTCTTTCAGA AGCTGGACCA CAATGCGGAC 
GAGTACGAGC TGCCCACCGT CAAGGACTAC TTCAGAGACC TCTTCTACAG GGTGGAGGTG 
ACCTTCTGTG ATAAATCTAT TGCCAATGAC CCCGGCTTCA CGATGGAGCT TTCTCTCAAG 
ATGAACTATG ACCAGATCGC CAGGGCGGTG GCGCAACGGC TAGGCACAGA CCCTTATTTA 
TTACAATTTT TCAAAGTTCA GAGTTACCGA GAGGGTCCCG GTAACCCTCT GAGGTGTACA 
TACGAGGGGA CGCTCAAGGA CCTCCTGGTA TTCTTCAAGC CCAGGCAGCC CAAGAAGATC 
TATTACCAGC AGCTGAGCAT ACGGATAAAC GAGCTAGAAA GCAAGAAGCA GTTCAAGTGC 
GTCTGGGTCA ACAGCAAGTT CAAGGAAGAG AAGGAGCTTG TGCTGTACCC GAACAAGAAT 
GGCTGCGTGA GCGACCTCCT GGATGAGGCA CGCAAGCAGG TGGAGCTCTC GGACGACGGC 
ACAGGTTCTG GCAGGCTACG ACTGATGGAG GTGATCAGCT ACAAGATCTT CTCGGTGCCC 
AAGGAGGACG TGCAGCTGGA CACACTGAAC CAGGCCAACT CGAAGACCTA CCGGGTGGAG 
GAGATCCCCC GGGAAGACCT GCAGACCGCG GACGACGAGA TCCTGGTGCC ATGCGCCCAC 
TTCCAGAAAG AGATCTTCTC TACCTTCGGG GTGCCCTTCT TCCTCAAGAT GAAGCACCGG 
GAACCCTTCA CGAAGGTCAA GGACAGGATT CAGAAGAAGC TGGACATCCC TGACAAGGAG 
TTTGAAAAGT ACAAGTTCGC CATTGTCGTG ATGGGGCGGG CGCAGTTCAT CGAGGATGCA 
GACTACGTGG TGAACCTGCA AGACTTCATG GCGCAACCTC CTCAAGGTGC GGCGATGCAC 
CCCCGTCCCT GGCTGGGCAT CGAACACATC AACAAGGCGC CCAAGAGGTC CCGCTACAAC 
TACCTGGAGA AGGCCATCAA GATCCACAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029834875.1
CDS	27..3779
Translation	MLRAAAIVSLGVPCKVRFVVAWFPLSGGLTPADIGRIASKHVKVTVSGKNPLAAGKERSNSSARSKRNAATRDTTAAVGLIMNHAVLHDKDSGQADLEEMDTQEGDENVAPPPSQQQQQPPQPTQQAQPLHHPPPPPPPETGGRERFANGVSPAVGDVHSPPPPAPPACDEPMAPAPPPAADAGALTKVPAAAAAPDGLDEPMEEEDEARSEATFRFVVTGFSRLRESVLSPATQVRNLPWKIMVMPRTNAGNDRTPTKSLGFFLQCNGESESSTWSCNATAELRIISQKEGVDNFVRKIQHLFYSKENDWGFSHFMTWTDVLDPEKGFILDDTIILEVWVSADAPHGVSWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYQMPTESDDSSKSVALALQRVFYELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNMESKMKGTCVEGTIPRLFEGKMISFIKCKHVEYTSRRMEPFYDIQLNVKGKKNIHESFQDYCATESLDGENKYDAGEYGLQEAEKGIIFACLPPVVHLHLLRFQYDPLTDNNVKLNDRFEFPEKLNLSDFLHEKGPSPATYTLHAVLVHSGDNHGGHYVVFINPRGDGRWCKFDDDVVSRCTKQEAIDHNFGGTEDDVAVSRHCTNAYMLVYIRDSAINEVLQSVTENDIPEQLVERLQEEKRQEALRRKERNEAHLYMSVQVLTEDSFAGHQGNDLYDPEKASYRSFKVKKQATLRDLLDLIADQMRFPLHAIRPWSITYRSNQTFRPAAVDLDADLNKTVIDLSENANPWTIFLETIVPDQPMDRLPEFDKETDVLLFFKMYDPRLKQIAYCGHTYMPITVKAKELVPLLNKRAGFPPDTELVLYEEVKPNIVDRIEDLELPLEKVLEELMDGDIIVFQKLDHNADEYELPTVKDYFRDLFYRVEVTFCDKSIANDPGFTMELSLKMNYDQIARAVAQRLGTDPYLLQFFKVQSYREGPGNPLRCTYEGTLKDLLVFFKPRQPKKIYYQQLSIRINELESKKQFKCVWVNSKFKEEKELVLYPNKNGCVSDLLDEARKQVELSDDGTGSGRLRLMEVISYKIFSVPKEDVQLDTLNQANSKTYRVEEIPREDLQTADDEILVPCAHFQKEIFSTFGVPFFLKMKHREPFTKVKDRIQKKLDIPDKEFEKYKFAIVVMGRAQFIEDADYVVNLQDFMAQPPQGAAMHPRPWLGIEHINKAPKRSRYNYLEKAIKIHN

Target ORF information:

RefSeq Version	XM_029979015.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029979015.1

ORF Insert Sequence:

ATGCTCCGTG CCGCCGCGAT CGTGAGTCTC GGCGTCCCCT GCAAAGTCCG TTTTGTCGTC 
GCATGGTTTC CCCTGTCCGG GGGGCTGACA CCAGCAGACA TTGGGCGAAT CGCGAGCAAG 
CACGTCAAAG TGACAGTGTC CGGCAAAAAC CCCCTGGCCG CAGGCAAAGA ACGCTCCAAC 
AGCTCTGCCC GATCTAAGCG CAACGCCGCC ACCCGTGACA CCACAGCTGC CGTCGGACTT 
ATAATGAACC ACGCTGTTCT TCATGACAAG GACAGCGGTC AGGCAGACTT GGAGGAAATG 
GATACACAAG AAGGTGACGA AAACGTGGCC CCTCCACCGT CGCAGCAGCA GCAGCAGCCG 
CCACAGCCGA CCCAGCAGGC CCAGCCCCTG CACCACCCGC CGCCCCCTCC CCCGCCTGAG 
ACGGGCGGGC GGGAGCGCTT TGCCAACGGC GTGTCCCCCG CGGTGGGCGA CGTGCACAGC 
CCGCCCCCTC CCGCTCCTCC CGCCTGCGAC GAGCCCATGG CGCCGGCCCC GCCCCCCGCC 
GCCGACGCAG GCGCGCTGAC GAAGGTGCCG GCGGCAGCAG CGGCGCCGGA TGGCCTCGAC 
GAGCCCATGG AGGAGGAGGA CGAGGCCCGG TCGGAGGCCA CCTTCCGCTT TGTGGTGACG 
GGCTTCTCGA GGCTGCGGGA GTCGGTGCTC AGTCCGGCCA CCCAAGTGCG CAACCTCCCC 
TGGAAAATCA TGGTGATGCC GCGCACGAAT GCGGGCAACG ACCGGACACC GACCAAGAGT 
CTGGGCTTCT TCCTACAATG CAACGGCGAG TCGGAGTCCT CGACGTGGTC ATGCAACGCG 
ACGGCGGAGC TTCGCATCAT CAGCCAGAAG GAGGGCGTCG ACAACTTCGT AAGAAAAATC 
CAGCACCTGT TCTATAGCAA GGAGAACGAC TGGGGATTCA GTCACTTCAT GACGTGGACG 
GACGTTCTCG ATCCGGAGAA AGGGTTCATT CTGGACGACA CCATCATCCT GGAGGTGTGG 
GTGTCGGCGG ACGCGCCTCA CGGGGTCAGC TGGGACTCCA AAAAGCACAC GGGCTACGTG 
GGCCTGAAGA ACCAGGGTGC CACGTGCTAC ATGAACTCCC TCCTGCAGAC ACTCTTCTTC 
ACGAATCAGC TCCGCAAGGC GGTGTACCAG ATGCCGACGG AGAGCGACGA CTCGTCCAAG 
AGCGTGGCGC TGGCGCTACA GCGGGTCTTC TACGAGCTGC AGTTCTCGGA CAAGCCCGTG 
GGCACCAAGA AGCTCACGAA GTCCTTCGGC TGGGAGACGC TCGACTCTTT TATGCAGCAC 
GACGTCCAGG AGCTGTGTAG AGTGCTGCTG GACAACATGG AGAGCAAGAT GAAGGGCACC 
TGCGTGGAGG GCACCATCCC CCGGCTCTTT GAGGGCAAGA TGATCTCGTT CATCAAGTGC 
AAGCACGTGG AGTACACCTC CAGGCGCATG GAGCCCTTCT ACGACATCCA GCTGAACGTC 
AAGGGCAAGA AGAACATCCA CGAGTCCTTC CAGGACTACT GCGCCACAGA GAGCCTGGAC 
GGGGAGAACA AGTACGACGC AGGGGAGTAC GGGCTGCAGG AGGCGGAGAA AGGCATCATT 
TTCGCCTGCC TGCCCCCCGT GGTGCACCTG CACCTGCTGC GCTTCCAGTA CGACCCCCTC 
ACGGACAACA ACGTCAAGCT CAACGACCGC TTTGAGTTTC CCGAGAAGCT CAACCTCTCA 
GACTTCCTCC ACGAGAAGGG CCCGAGCCCG GCGACGTACA CCCTGCACGC GGTGCTGGTG 
CACTCCGGGG ACAACCACGG GGGCCACTAC GTGGTGTTCA TCAACCCCCG GGGGGACGGG 
CGCTGGTGCA AGTTCGACGA CGACGTGGTC TCGCGCTGCA CCAAGCAGGA GGCCATCGAC 
CACAACTTCG GGGGCACGGA GGACGACGTG GCCGTCAGCC GCCACTGCAC CAATGCCTAC 
ATGCTGGTGT ACATCAGGGA CAGCGCCATC AACGAGGTCC TGCAGTCGGT GACGGAAAAC 
GACATCCCCG AGCAGCTGGT CGAGAGGCTT CAGGAGGAAA AGCGCCAGGA GGCCCTGCGG 
CGCAAGGAGC GCAACGAGGC GCACCTGTAC ATGAGCGTGC AGGTGCTGAC GGAGGATAGC 
TTTGCGGGAC ACCAGGGCAA CGACCTGTAC GACCCGGAGA AGGCCTCTTA CCGCTCCTTC 
AAGGTGAAGA AGCAAGCCAC CCTGCGGGAC CTGCTGGACC TGATTGCAGA CCAGATGCGC 
TTCCCCCTGC ACGCCATCCG GCCCTGGTCC ATCACCTACC GCTCCAACCA AACCTTCCGG 
CCGGCCGCCG TGGACCTTGA CGCGGACCTC AACAAGACGG TGATTGACCT GTCCGAGAAT 
GCCAACCCCT GGACGATCTT CCTGGAGACG ATCGTGCCGG ACCAGCCGAT GGACCGGTTG 
CCGGAGTTCG ACAAAGAGAC GGACGTGCTG CTCTTCTTCA AGATGTACGA CCCAAGGCTG 
AAGCAGATCG CCTACTGCGG CCACACCTAC ATGCCCATCA CCGTCAAGGC CAAGGAGCTG 
GTGCCGCTGC TCAACAAGAG GGCGGGGTTT CCTCCTGACA CGGAGCTGGT GCTCTACGAG 
GAGGTGAAGC CAAACATTGT GGACCGCATT GAGGACCTGG AGCTGCCCCT GGAGAAGGTC 
CTGGAGGAGC TGATGGACGG AGACATAATC GTCTTTCAGA AGCTGGACCA CAATGCGGAC 
GAGTACGAGC TGCCCACCGT CAAGGACTAC TTCAGAGACC TCTTCTACAG GGTGGAGGTG 
ACCTTCTGTG ATAAATCTAT TGCCAATGAC CCCGGCTTCA CGATGGAGCT TTCTCTCAAG 
ATGAACTATG ACCAGATCGC CAGGGCGGTG GCGCAACGGC TAGGCACAGA CCCTTATTTA 
TTACAATTTT TCAAAGTTCA GAGTTACCGA GAGGGTCCCG GTAACCCTCT GAGGTGTACA 
TACGAGGGGA CGCTCAAGGA CCTCCTGGTA TTCTTCAAGC CCAGGCAGCC CAAGAAGATC 
TATTACCAGC AGCTGAGCAT ACGGATAAAC GAGCTAGAAA GCAAGAAGCA GTTCAAGTGC 
GTCTGGGTCA ACAGCAAGTT CAAGGAAGAG AAGGAGCTTG TGCTGTACCC GAACAAGAAT 
GGCTGCGTGA GCGACCTCCT GGATGAGGCA CGCAAGCAGG TGGAGCTCTC GGACGACGGC 
ACAGGTTCTG GCAGGCTACG ACTGATGGAG GTGATCAGCT ACAAGATCTT CTCGGTGCCC 
AAGGAGGACG TGCAGCTGGA CACACTGAAC CAGGCCAACT CGAAGACCTA CCGGGTGGAG 
GAGATCCCCC GGGAAGACCT GCAGACCGCG GACGACGAGA TCCTGGTGCC ATGCGCCCAC 
TTCCAGAAAG AGATCTTCTC TACCTTCGGG GTGCCCTTCT TCCTCAAGAT GAAGCACCGG 
GAACCCTTCA CGAAGGTCAA GGACAGGATT CAGAAGAAGC TGGACATCCC TGACAAGGAG 
TTTGAAAAGT ACAAGTTCGC CATTGTCGTG ATGGGGCGGG CGCAGTTCAT CGAGGATGCA 
GACTACGTGG TGAACCTGCA AGACTTCATG GCGCAACCTC CTCAAGGTGC GGCGATGCAC 
CCCCGTCCCT GGCTGGGCAT CGAACACATC AACAAGGCGC CCAAGAGGTC CCGCTACAAC 
TACCTGGAGA AGGCCATCAA GATCCACAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8024169 ( XM_029979016.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029834876.1 Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X2, mRNA.

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CloneID	OIa22177
Clone ID Related Accession (Same CDS sequence)	XM_029979015.1 , XM_029979016.1
Accession Version	XM_029979016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGTG CCGCCGCGAT CGTGAGTCTC GGCGTCCCCT GCAAAGTCCG TTTTGTCGTC 
GCATGGTTTC CCCTGTCCGG GGGGCTGACA CCAGCAGACA TTGGGCGAAT CGCGAGCAAG 
CACGTCAAAG TGACAGTGTC CGGCAAAAAC CCCCTGGCCG CAGGCAAAGA ACGCTCCAAC 
AGCTCTGCCC GATCTAAGCG CAACGCCGCC ACCCGTGACA CCACAGCTGC CGTCGGACTT 
ATAATGAACC ACGCTGTTCT TCATGACAAG GACAGCGGTC AGGCAGACTT GGAGGAAATG 
GATACACAAG AAGGTGACGA AAACGTGGCC CCTCCACCGT CGCAGCAGCA GCAGCAGCCG 
CCACAGCCGA CCCAGCAGGC CCAGCCCCTG CACCACCCGC CGCCCCCTCC CCCGCCTGAG 
ACGGGCGGGC GGGAGCGCTT TGCCAACGGC GTGTCCCCCG CGGTGGGCGA CGTGCACAGC 
CCGCCCCCTC CCGCTCCTCC CGCCTGCGAC GAGCCCATGG CGCCGGCCCC GCCCCCCGCC 
GCCGACGCAG GCGCGCTGAC GAAGGTGCCG GCGGCAGCAG CGGCGCCGGA TGGCCTCGAC 
GAGCCCATGG AGGAGGAGGA CGAGGCCCGG TCGGAGGCCA CCTTCCGCTT TGTGGTGACG 
GGCTTCTCGA GGCTGCGGGA GTCGGTGCTC AGTCCGGCCA CCCAAGTGCG CAACCTCCCC 
TGGAAAATCA TGGTGATGCC GCGCACGAAT GCGGGCAACG ACCGGACACC GACCAAGAGT 
CTGGGCTTCT TCCTACAATG CAACGGCGAG TCGGAGTCCT CGACGTGGTC ATGCAACGCG 
ACGGCGGAGC TTCGCATCAT CAGCCAGAAG GAGGGCGTCG ACAACTTCGT AAGAAAAATC 
CAGCACCTGT TCTATAGCAA GGAGAACGAC TGGGGATTCA GTCACTTCAT GACGTGGACG 
GACGTTCTCG ATCCGGAGAA AGGGTTCATT CTGGACGACA CCATCATCCT GGAGGTGTGG 
GTGTCGGCGG ACGCGCCTCA CGGGGTCAGC TGGGACTCCA AAAAGCACAC GGGCTACGTG 
GGCCTGAAGA ACCAGGGTGC CACGTGCTAC ATGAACTCCC TCCTGCAGAC ACTCTTCTTC 
ACGAATCAGC TCCGCAAGGC GGTGTACCAG ATGCCGACGG AGAGCGACGA CTCGTCCAAG 
AGCGTGGCGC TGGCGCTACA GCGGGTCTTC TACGAGCTGC AGTTCTCGGA CAAGCCCGTG 
GGCACCAAGA AGCTCACGAA GTCCTTCGGC TGGGAGACGC TCGACTCTTT TATGCAGCAC 
GACGTCCAGG AGCTGTGTAG AGTGCTGCTG GACAACATGG AGAGCAAGAT GAAGGGCACC 
TGCGTGGAGG GCACCATCCC CCGGCTCTTT GAGGGCAAGA TGATCTCGTT CATCAAGTGC 
AAGCACGTGG AGTACACCTC CAGGCGCATG GAGCCCTTCT ACGACATCCA GCTGAACGTC 
AAGGGCAAGA AGAACATCCA CGAGTCCTTC CAGGACTACT GCGCCACAGA GAGCCTGGAC 
GGGGAGAACA AGTACGACGC AGGGGAGTAC GGGCTGCAGG AGGCGGAGAA AGGCATCATT 
TTCGCCTGCC TGCCCCCCGT GGTGCACCTG CACCTGCTGC GCTTCCAGTA CGACCCCCTC 
ACGGACAACA ACGTCAAGCT CAACGACCGC TTTGAGTTTC CCGAGAAGCT CAACCTCTCA 
GACTTCCTCC ACGAGAAGGG CCCGAGCCCG GCGACGTACA CCCTGCACGC GGTGCTGGTG 
CACTCCGGGG ACAACCACGG GGGCCACTAC GTGGTGTTCA TCAACCCCCG GGGGGACGGG 
CGCTGGTGCA AGTTCGACGA CGACGTGGTC TCGCGCTGCA CCAAGCAGGA GGCCATCGAC 
CACAACTTCG GGGGCACGGA GGACGACGTG GCCGTCAGCC GCCACTGCAC CAATGCCTAC 
ATGCTGGTGT ACATCAGGGA CAGCGCCATC AACGAGGTCC TGCAGTCGGT GACGGAAAAC 
GACATCCCCG AGCAGCTGGT CGAGAGGCTT CAGGAGGAAA AGCGCCAGGA GGCCCTGCGG 
CGCAAGGAGC GCAACGAGGC GCACCTGTAC ATGAGCGTGC AGGTGCTGAC GGAGGATAGC 
TTTGCGGGAC ACCAGGGCAA CGACCTGTAC GACCCGGAGA AGGCCTCTTA CCGCTCCTTC 
AAGGTGAAGA AGCAAGCCAC CCTGCGGGAC CTGCTGGACC TGATTGCAGA CCAGATGCGC 
TTCCCCCTGC ACGCCATCCG GCCCTGGTCC ATCACCTACC GCTCCAACCA AACCTTCCGG 
CCGGCCGCCG TGGACCTTGA CGCGGACCTC AACAAGACGG TGATTGACCT GTCCGAGAAT 
GCCAACCCCT GGACGATCTT CCTGGAGACG ATCGTGCCGG ACCAGCCGAT GGACCGGTTG 
CCGGAGTTCG ACAAAGAGAC GGACGTGCTG CTCTTCTTCA AGATGTACGA CCCAAGGCTG 
AAGCAGATCG CCTACTGCGG CCACACCTAC ATGCCCATCA CCGTCAAGGC CAAGGAGCTG 
GTGCCGCTGC TCAACAAGAG GGCGGGGTTT CCTCCTGACA CGGAGCTGGT GCTCTACGAG 
GAGGTGAAGC CAAACATTGT GGACCGCATT GAGGACCTGG AGCTGCCCCT GGAGAAGGTC 
CTGGAGGAGC TGATGGACGG AGACATAATC GTCTTTCAGA AGCTGGACCA CAATGCGGAC 
GAGTACGAGC TGCCCACCGT CAAGGACTAC TTCAGAGACC TCTTCTACAG GGTGGAGGTG 
ACCTTCTGTG ATAAATCTAT TGCCAATGAC CCCGGCTTCA CGATGGAGCT TTCTCTCAAG 
ATGAACTATG ACCAGATCGC CAGGGCGGTG GCGCAACGGC TAGGCACAGA CCCTTATTTA 
TTACAATTTT TCAAAGTTCA GAGTTACCGA GAGGGTCCCG GTAACCCTCT GAGGTGTACA 
TACGAGGGGA CGCTCAAGGA CCTCCTGGTA TTCTTCAAGC CCAGGCAGCC CAAGAAGATC 
TATTACCAGC AGCTGAGCAT ACGGATAAAC GAGCTAGAAA GCAAGAAGCA GTTCAAGTGC 
GTCTGGGTCA ACAGCAAGTT CAAGGAAGAG AAGGAGCTTG TGCTGTACCC GAACAAGAAT 
GGCTGCGTGA GCGACCTCCT GGATGAGGCA CGCAAGCAGG TGGAGCTCTC GGACGACGGC 
ACAGGTTCTG GCAGGCTACG ACTGATGGAG GTGATCAGCT ACAAGATCTT CTCGGTGCCC 
AAGGAGGACG TGCAGCTGGA CACACTGAAC CAGGCCAACT CGAAGACCTA CCGGGTGGAG 
GAGATCCCCC GGGAAGACCT GCAGACCGCG GACGACGAGA TCCTGGTGCC ATGCGCCCAC 
TTCCAGAAAG AGATCTTCTC TACCTTCGGG GTGCCCTTCT TCCTCAAGAT GAAGCACCGG 
GAACCCTTCA CGAAGGTCAA GGACAGGATT CAGAAGAAGC TGGACATCCC TGACAAGGAG 
TTTGAAAAGT ACAAGTTCGC CATTGTCGTG ATGGGGCGGG CGCAGTTCAT CGAGGATGCA 
GACTACGTGG TGAACCTGCA AGACTTCATG GCGCAACCTC CTCAAGGTGC GGCGATGCAC 
CCCCGTCCCT GGCTGGGCAT CGAACACATC AACAAGGCGC CCAAGAGGTC CCGCTACAAC 
TACCTGGAGA AGGCCATCAA GATCCACAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029834876.1
CDS	27..3779
Translation	MLRAAAIVSLGVPCKVRFVVAWFPLSGGLTPADIGRIASKHVKVTVSGKNPLAAGKERSNSSARSKRNAATRDTTAAVGLIMNHAVLHDKDSGQADLEEMDTQEGDENVAPPPSQQQQQPPQPTQQAQPLHHPPPPPPPETGGRERFANGVSPAVGDVHSPPPPAPPACDEPMAPAPPPAADAGALTKVPAAAAAPDGLDEPMEEEDEARSEATFRFVVTGFSRLRESVLSPATQVRNLPWKIMVMPRTNAGNDRTPTKSLGFFLQCNGESESSTWSCNATAELRIISQKEGVDNFVRKIQHLFYSKENDWGFSHFMTWTDVLDPEKGFILDDTIILEVWVSADAPHGVSWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYQMPTESDDSSKSVALALQRVFYELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNMESKMKGTCVEGTIPRLFEGKMISFIKCKHVEYTSRRMEPFYDIQLNVKGKKNIHESFQDYCATESLDGENKYDAGEYGLQEAEKGIIFACLPPVVHLHLLRFQYDPLTDNNVKLNDRFEFPEKLNLSDFLHEKGPSPATYTLHAVLVHSGDNHGGHYVVFINPRGDGRWCKFDDDVVSRCTKQEAIDHNFGGTEDDVAVSRHCTNAYMLVYIRDSAINEVLQSVTENDIPEQLVERLQEEKRQEALRRKERNEAHLYMSVQVLTEDSFAGHQGNDLYDPEKASYRSFKVKKQATLRDLLDLIADQMRFPLHAIRPWSITYRSNQTFRPAAVDLDADLNKTVIDLSENANPWTIFLETIVPDQPMDRLPEFDKETDVLLFFKMYDPRLKQIAYCGHTYMPITVKAKELVPLLNKRAGFPPDTELVLYEEVKPNIVDRIEDLELPLEKVLEELMDGDIIVFQKLDHNADEYELPTVKDYFRDLFYRVEVTFCDKSIANDPGFTMELSLKMNYDQIARAVAQRLGTDPYLLQFFKVQSYREGPGNPLRCTYEGTLKDLLVFFKPRQPKKIYYQQLSIRINELESKKQFKCVWVNSKFKEEKELVLYPNKNGCVSDLLDEARKQVELSDDGTGSGRLRLMEVISYKIFSVPKEDVQLDTLNQANSKTYRVEEIPREDLQTADDEILVPCAHFQKEIFSTFGVPFFLKMKHREPFTKVKDRIQKKLDIPDKEFEKYKFAIVVMGRAQFIEDADYVVNLQDFMAQPPQGAAMHPRPWLGIEHINKAPKRSRYNYLEKAIKIHN

Target ORF information:

RefSeq Version	XM_029979016.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029979016.1

ORF Insert Sequence:

ATGCTCCGTG CCGCCGCGAT CGTGAGTCTC GGCGTCCCCT GCAAAGTCCG TTTTGTCGTC 
GCATGGTTTC CCCTGTCCGG GGGGCTGACA CCAGCAGACA TTGGGCGAAT CGCGAGCAAG 
CACGTCAAAG TGACAGTGTC CGGCAAAAAC CCCCTGGCCG CAGGCAAAGA ACGCTCCAAC 
AGCTCTGCCC GATCTAAGCG CAACGCCGCC ACCCGTGACA CCACAGCTGC CGTCGGACTT 
ATAATGAACC ACGCTGTTCT TCATGACAAG GACAGCGGTC AGGCAGACTT GGAGGAAATG 
GATACACAAG AAGGTGACGA AAACGTGGCC CCTCCACCGT CGCAGCAGCA GCAGCAGCCG 
CCACAGCCGA CCCAGCAGGC CCAGCCCCTG CACCACCCGC CGCCCCCTCC CCCGCCTGAG 
ACGGGCGGGC GGGAGCGCTT TGCCAACGGC GTGTCCCCCG CGGTGGGCGA CGTGCACAGC 
CCGCCCCCTC CCGCTCCTCC CGCCTGCGAC GAGCCCATGG CGCCGGCCCC GCCCCCCGCC 
GCCGACGCAG GCGCGCTGAC GAAGGTGCCG GCGGCAGCAG CGGCGCCGGA TGGCCTCGAC 
GAGCCCATGG AGGAGGAGGA CGAGGCCCGG TCGGAGGCCA CCTTCCGCTT TGTGGTGACG 
GGCTTCTCGA GGCTGCGGGA GTCGGTGCTC AGTCCGGCCA CCCAAGTGCG CAACCTCCCC 
TGGAAAATCA TGGTGATGCC GCGCACGAAT GCGGGCAACG ACCGGACACC GACCAAGAGT 
CTGGGCTTCT TCCTACAATG CAACGGCGAG TCGGAGTCCT CGACGTGGTC ATGCAACGCG 
ACGGCGGAGC TTCGCATCAT CAGCCAGAAG GAGGGCGTCG ACAACTTCGT AAGAAAAATC 
CAGCACCTGT TCTATAGCAA GGAGAACGAC TGGGGATTCA GTCACTTCAT GACGTGGACG 
GACGTTCTCG ATCCGGAGAA AGGGTTCATT CTGGACGACA CCATCATCCT GGAGGTGTGG 
GTGTCGGCGG ACGCGCCTCA CGGGGTCAGC TGGGACTCCA AAAAGCACAC GGGCTACGTG 
GGCCTGAAGA ACCAGGGTGC CACGTGCTAC ATGAACTCCC TCCTGCAGAC ACTCTTCTTC 
ACGAATCAGC TCCGCAAGGC GGTGTACCAG ATGCCGACGG AGAGCGACGA CTCGTCCAAG 
AGCGTGGCGC TGGCGCTACA GCGGGTCTTC TACGAGCTGC AGTTCTCGGA CAAGCCCGTG 
GGCACCAAGA AGCTCACGAA GTCCTTCGGC TGGGAGACGC TCGACTCTTT TATGCAGCAC 
GACGTCCAGG AGCTGTGTAG AGTGCTGCTG GACAACATGG AGAGCAAGAT GAAGGGCACC 
TGCGTGGAGG GCACCATCCC CCGGCTCTTT GAGGGCAAGA TGATCTCGTT CATCAAGTGC 
AAGCACGTGG AGTACACCTC CAGGCGCATG GAGCCCTTCT ACGACATCCA GCTGAACGTC 
AAGGGCAAGA AGAACATCCA CGAGTCCTTC CAGGACTACT GCGCCACAGA GAGCCTGGAC 
GGGGAGAACA AGTACGACGC AGGGGAGTAC GGGCTGCAGG AGGCGGAGAA AGGCATCATT 
TTCGCCTGCC TGCCCCCCGT GGTGCACCTG CACCTGCTGC GCTTCCAGTA CGACCCCCTC 
ACGGACAACA ACGTCAAGCT CAACGACCGC TTTGAGTTTC CCGAGAAGCT CAACCTCTCA 
GACTTCCTCC ACGAGAAGGG CCCGAGCCCG GCGACGTACA CCCTGCACGC GGTGCTGGTG 
CACTCCGGGG ACAACCACGG GGGCCACTAC GTGGTGTTCA TCAACCCCCG GGGGGACGGG 
CGCTGGTGCA AGTTCGACGA CGACGTGGTC TCGCGCTGCA CCAAGCAGGA GGCCATCGAC 
CACAACTTCG GGGGCACGGA GGACGACGTG GCCGTCAGCC GCCACTGCAC CAATGCCTAC 
ATGCTGGTGT ACATCAGGGA CAGCGCCATC AACGAGGTCC TGCAGTCGGT GACGGAAAAC 
GACATCCCCG AGCAGCTGGT CGAGAGGCTT CAGGAGGAAA AGCGCCAGGA GGCCCTGCGG 
CGCAAGGAGC GCAACGAGGC GCACCTGTAC ATGAGCGTGC AGGTGCTGAC GGAGGATAGC 
TTTGCGGGAC ACCAGGGCAA CGACCTGTAC GACCCGGAGA AGGCCTCTTA CCGCTCCTTC 
AAGGTGAAGA AGCAAGCCAC CCTGCGGGAC CTGCTGGACC TGATTGCAGA CCAGATGCGC 
TTCCCCCTGC ACGCCATCCG GCCCTGGTCC ATCACCTACC GCTCCAACCA AACCTTCCGG 
CCGGCCGCCG TGGACCTTGA CGCGGACCTC AACAAGACGG TGATTGACCT GTCCGAGAAT 
GCCAACCCCT GGACGATCTT CCTGGAGACG ATCGTGCCGG ACCAGCCGAT GGACCGGTTG 
CCGGAGTTCG ACAAAGAGAC GGACGTGCTG CTCTTCTTCA AGATGTACGA CCCAAGGCTG 
AAGCAGATCG CCTACTGCGG CCACACCTAC ATGCCCATCA CCGTCAAGGC CAAGGAGCTG 
GTGCCGCTGC TCAACAAGAG GGCGGGGTTT CCTCCTGACA CGGAGCTGGT GCTCTACGAG 
GAGGTGAAGC CAAACATTGT GGACCGCATT GAGGACCTGG AGCTGCCCCT GGAGAAGGTC 
CTGGAGGAGC TGATGGACGG AGACATAATC GTCTTTCAGA AGCTGGACCA CAATGCGGAC 
GAGTACGAGC TGCCCACCGT CAAGGACTAC TTCAGAGACC TCTTCTACAG GGTGGAGGTG 
ACCTTCTGTG ATAAATCTAT TGCCAATGAC CCCGGCTTCA CGATGGAGCT TTCTCTCAAG 
ATGAACTATG ACCAGATCGC CAGGGCGGTG GCGCAACGGC TAGGCACAGA CCCTTATTTA 
TTACAATTTT TCAAAGTTCA GAGTTACCGA GAGGGTCCCG GTAACCCTCT GAGGTGTACA 
TACGAGGGGA CGCTCAAGGA CCTCCTGGTA TTCTTCAAGC CCAGGCAGCC CAAGAAGATC 
TATTACCAGC AGCTGAGCAT ACGGATAAAC GAGCTAGAAA GCAAGAAGCA GTTCAAGTGC 
GTCTGGGTCA ACAGCAAGTT CAAGGAAGAG AAGGAGCTTG TGCTGTACCC GAACAAGAAT 
GGCTGCGTGA GCGACCTCCT GGATGAGGCA CGCAAGCAGG TGGAGCTCTC GGACGACGGC 
ACAGGTTCTG GCAGGCTACG ACTGATGGAG GTGATCAGCT ACAAGATCTT CTCGGTGCCC 
AAGGAGGACG TGCAGCTGGA CACACTGAAC CAGGCCAACT CGAAGACCTA CCGGGTGGAG 
GAGATCCCCC GGGAAGACCT GCAGACCGCG GACGACGAGA TCCTGGTGCC ATGCGCCCAC 
TTCCAGAAAG AGATCTTCTC TACCTTCGGG GTGCCCTTCT TCCTCAAGAT GAAGCACCGG 
GAACCCTTCA CGAAGGTCAA GGACAGGATT CAGAAGAAGC TGGACATCCC TGACAAGGAG 
TTTGAAAAGT ACAAGTTCGC CATTGTCGTG ATGGGGCGGG CGCAGTTCAT CGAGGATGCA 
GACTACGTGG TGAACCTGCA AGACTTCATG GCGCAACCTC CTCAAGGTGC GGCGATGCAC 
CCCCGTCCCT GGCTGGGCAT CGAACACATC AACAAGGCGC CCAAGAGGTC CCGCTACAAC 
TACCTGGAGA AGGCCATCAA GATCCACAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8024169 ( XM_029979017.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029834877.1 Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X3, mRNA.

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CloneID	OIa22178
Clone ID Related Accession (Same CDS sequence)	XM_029979017.1
Accession Version	XM_029979017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGTG CCGCCGCGAT CGTGAGTCTC GGCGTCCCCT GCAAAGTCCG TTTTGTCGTC 
GCATGGTTTC CCCTGTCCGG GGGGCTGACA CCAGCAGACA TTGGGCGAAT CGCGAGCAAG 
CACGTCAAAG TGACAGTGTC CGGCAAAAAC CCCCTGGCCG CAGGCAAAGA ACGCTCCAAC 
AGCTCTGCCC GATCTAAGCG CAACGCCGCC ACCCGTGACA CCACAGCTGC CGTCGGACTT 
ATAATGAACC ACGCTGTTCT TCATGACAAG GACAGCGGTC AGGCAGACTT GGAGGAAATG 
GATACACAAG AAGACGAAAA CGTGGCCCCT CCACCGTCGC AGCAGCAGCA GCAGCCGCCA 
CAGCCGACCC AGCAGGCCCA GCCCCTGCAC CACCCGCCGC CCCCTCCCCC GCCTGAGACG 
GGCGGGCGGG AGCGCTTTGC CAACGGCGTG TCCCCCGCGG TGGGCGACGT GCACAGCCCG 
CCCCCTCCCG CTCCTCCCGC CTGCGACGAG CCCATGGCGC CGGCCCCGCC CCCCGCCGCC 
GACGCAGGCG CGCTGACGAA GGTGCCGGCG GCAGCAGCGG CGCCGGATGG CCTCGACGAG 
CCCATGGAGG AGGAGGACGA GGCCCGGTCG GAGGCCACCT TCCGCTTTGT GGTGACGGGC 
TTCTCGAGGC TGCGGGAGTC GGTGCTCAGT CCGGCCACCC AAGTGCGCAA CCTCCCCTGG 
AAAATCATGG TGATGCCGCG CACGAATGCG GGCAACGACC GGACACCGAC CAAGAGTCTG 
GGCTTCTTCC TACAATGCAA CGGCGAGTCG GAGTCCTCGA CGTGGTCATG CAACGCGACG 
GCGGAGCTTC GCATCATCAG CCAGAAGGAG GGCGTCGACA ACTTCGTAAG AAAAATCCAG 
CACCTGTTCT ATAGCAAGGA GAACGACTGG GGATTCAGTC ACTTCATGAC GTGGACGGAC 
GTTCTCGATC CGGAGAAAGG GTTCATTCTG GACGACACCA TCATCCTGGA GGTGTGGGTG 
TCGGCGGACG CGCCTCACGG GGTCAGCTGG GACTCCAAAA AGCACACGGG CTACGTGGGC 
CTGAAGAACC AGGGTGCCAC GTGCTACATG AACTCCCTCC TGCAGACACT CTTCTTCACG 
AATCAGCTCC GCAAGGCGGT GTACCAGATG CCGACGGAGA GCGACGACTC GTCCAAGAGC 
GTGGCGCTGG CGCTACAGCG GGTCTTCTAC GAGCTGCAGT TCTCGGACAA GCCCGTGGGC 
ACCAAGAAGC TCACGAAGTC CTTCGGCTGG GAGACGCTCG ACTCTTTTAT GCAGCACGAC 
GTCCAGGAGC TGTGTAGAGT GCTGCTGGAC AACATGGAGA GCAAGATGAA GGGCACCTGC 
GTGGAGGGCA CCATCCCCCG GCTCTTTGAG GGCAAGATGA TCTCGTTCAT CAAGTGCAAG 
CACGTGGAGT ACACCTCCAG GCGCATGGAG CCCTTCTACG ACATCCAGCT GAACGTCAAG 
GGCAAGAAGA ACATCCACGA GTCCTTCCAG GACTACTGCG CCACAGAGAG CCTGGACGGG 
GAGAACAAGT ACGACGCAGG GGAGTACGGG CTGCAGGAGG CGGAGAAAGG CATCATTTTC 
GCCTGCCTGC CCCCCGTGGT GCACCTGCAC CTGCTGCGCT TCCAGTACGA CCCCCTCACG 
GACAACAACG TCAAGCTCAA CGACCGCTTT GAGTTTCCCG AGAAGCTCAA CCTCTCAGAC 
TTCCTCCACG AGAAGGGCCC GAGCCCGGCG ACGTACACCC TGCACGCGGT GCTGGTGCAC 
TCCGGGGACA ACCACGGGGG CCACTACGTG GTGTTCATCA ACCCCCGGGG GGACGGGCGC 
TGGTGCAAGT TCGACGACGA CGTGGTCTCG CGCTGCACCA AGCAGGAGGC CATCGACCAC 
AACTTCGGGG GCACGGAGGA CGACGTGGCC GTCAGCCGCC ACTGCACCAA TGCCTACATG 
CTGGTGTACA TCAGGGACAG CGCCATCAAC GAGGTCCTGC AGTCGGTGAC GGAAAACGAC 
ATCCCCGAGC AGCTGGTCGA GAGGCTTCAG GAGGAAAAGC GCCAGGAGGC CCTGCGGCGC 
AAGGAGCGCA ACGAGGCGCA CCTGTACATG AGCGTGCAGG TGCTGACGGA GGATAGCTTT 
GCGGGACACC AGGGCAACGA CCTGTACGAC CCGGAGAAGG CCTCTTACCG CTCCTTCAAG 
GTGAAGAAGC AAGCCACCCT GCGGGACCTG CTGGACCTGA TTGCAGACCA GATGCGCTTC 
CCCCTGCACG CCATCCGGCC CTGGTCCATC ACCTACCGCT CCAACCAAAC CTTCCGGCCG 
GCCGCCGTGG ACCTTGACGC GGACCTCAAC AAGACGGTGA TTGACCTGTC CGAGAATGCC 
AACCCCTGGA CGATCTTCCT GGAGACGATC GTGCCGGACC AGCCGATGGA CCGGTTGCCG 
GAGTTCGACA AAGAGACGGA CGTGCTGCTC TTCTTCAAGA TGTACGACCC AAGGCTGAAG 
CAGATCGCCT ACTGCGGCCA CACCTACATG CCCATCACCG TCAAGGCCAA GGAGCTGGTG 
CCGCTGCTCA ACAAGAGGGC GGGGTTTCCT CCTGACACGG AGCTGGTGCT CTACGAGGAG 
GTGAAGCCAA ACATTGTGGA CCGCATTGAG GACCTGGAGC TGCCCCTGGA GAAGGTCCTG 
GAGGAGCTGA TGGACGGAGA CATAATCGTC TTTCAGAAGC TGGACCACAA TGCGGACGAG 
TACGAGCTGC CCACCGTCAA GGACTACTTC AGAGACCTCT TCTACAGGGT GGAGGTGACC 
TTCTGTGATA AATCTATTGC CAATGACCCC GGCTTCACGA TGGAGCTTTC TCTCAAGATG 
AACTATGACC AGATCGCCAG GGCGGTGGCG CAACGGCTAG GCACAGACCC TTATTTATTA 
CAATTTTTCA AAGTTCAGAG TTACCGAGAG GGTCCCGGTA ACCCTCTGAG GTGTACATAC 
GAGGGGACGC TCAAGGACCT CCTGGTATTC TTCAAGCCCA GGCAGCCCAA GAAGATCTAT 
TACCAGCAGC TGAGCATACG GATAAACGAG CTAGAAAGCA AGAAGCAGTT CAAGTGCGTC 
TGGGTCAACA GCAAGTTCAA GGAAGAGAAG GAGCTTGTGC TGTACCCGAA CAAGAATGGC 
TGCGTGAGCG ACCTCCTGGA TGAGGCACGC AAGCAGGTGG AGCTCTCGGA CGACGGCACA 
GGTTCTGGCA GGCTACGACT GATGGAGGTG ATCAGCTACA AGATCTTCTC GGTGCCCAAG 
GAGGACGTGC AGCTGGACAC ACTGAACCAG GCCAACTCGA AGACCTACCG GGTGGAGGAG 
ATCCCCCGGG AAGACCTGCA GACCGCGGAC GACGAGATCC TGGTGCCATG CGCCCACTTC 
CAGAAAGAGA TCTTCTCTAC CTTCGGGGTG CCCTTCTTCC TCAAGATGAA GCACCGGGAA 
CCCTTCACGA AGGTCAAGGA CAGGATTCAG AAGAAGCTGG ACATCCCTGA CAAGGAGTTT 
GAAAAGTACA AGTTCGCCAT TGTCGTGATG GGGCGGGCGC AGTTCATCGA GGATGCAGAC 
TACGTGGTGA ACCTGCAAGA CTTCATGGCG CAACCTCCTC AAGGTGCGGC GATGCACCCC 
CGTCCCTGGC TGGGCATCGA ACACATCAAC AAGGCGCCCA AGAGGTCCCG CTACAACTAC 
CTGGAGAAGG CCATCAAGAT CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029834877.1
CDS	27..3776
Translation	MLRAAAIVSLGVPCKVRFVVAWFPLSGGLTPADIGRIASKHVKVTVSGKNPLAAGKERSNSSARSKRNAATRDTTAAVGLIMNHAVLHDKDSGQADLEEMDTQEDENVAPPPSQQQQQPPQPTQQAQPLHHPPPPPPPETGGRERFANGVSPAVGDVHSPPPPAPPACDEPMAPAPPPAADAGALTKVPAAAAAPDGLDEPMEEEDEARSEATFRFVVTGFSRLRESVLSPATQVRNLPWKIMVMPRTNAGNDRTPTKSLGFFLQCNGESESSTWSCNATAELRIISQKEGVDNFVRKIQHLFYSKENDWGFSHFMTWTDVLDPEKGFILDDTIILEVWVSADAPHGVSWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYQMPTESDDSSKSVALALQRVFYELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNMESKMKGTCVEGTIPRLFEGKMISFIKCKHVEYTSRRMEPFYDIQLNVKGKKNIHESFQDYCATESLDGENKYDAGEYGLQEAEKGIIFACLPPVVHLHLLRFQYDPLTDNNVKLNDRFEFPEKLNLSDFLHEKGPSPATYTLHAVLVHSGDNHGGHYVVFINPRGDGRWCKFDDDVVSRCTKQEAIDHNFGGTEDDVAVSRHCTNAYMLVYIRDSAINEVLQSVTENDIPEQLVERLQEEKRQEALRRKERNEAHLYMSVQVLTEDSFAGHQGNDLYDPEKASYRSFKVKKQATLRDLLDLIADQMRFPLHAIRPWSITYRSNQTFRPAAVDLDADLNKTVIDLSENANPWTIFLETIVPDQPMDRLPEFDKETDVLLFFKMYDPRLKQIAYCGHTYMPITVKAKELVPLLNKRAGFPPDTELVLYEEVKPNIVDRIEDLELPLEKVLEELMDGDIIVFQKLDHNADEYELPTVKDYFRDLFYRVEVTFCDKSIANDPGFTMELSLKMNYDQIARAVAQRLGTDPYLLQFFKVQSYREGPGNPLRCTYEGTLKDLLVFFKPRQPKKIYYQQLSIRINELESKKQFKCVWVNSKFKEEKELVLYPNKNGCVSDLLDEARKQVELSDDGTGSGRLRLMEVISYKIFSVPKEDVQLDTLNQANSKTYRVEEIPREDLQTADDEILVPCAHFQKEIFSTFGVPFFLKMKHREPFTKVKDRIQKKLDIPDKEFEKYKFAIVVMGRAQFIEDADYVVNLQDFMAQPPQGAAMHPRPWLGIEHINKAPKRSRYNYLEKAIKIHN

Target ORF information:

RefSeq Version	XM_029979017.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ubiquitin carboxyl-terminal hydrolase 7 (LOC8024169), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029979017.1

ORF Insert Sequence:

ATGCTCCGTG CCGCCGCGAT CGTGAGTCTC GGCGTCCCCT GCAAAGTCCG TTTTGTCGTC 
GCATGGTTTC CCCTGTCCGG GGGGCTGACA CCAGCAGACA TTGGGCGAAT CGCGAGCAAG 
CACGTCAAAG TGACAGTGTC CGGCAAAAAC CCCCTGGCCG CAGGCAAAGA ACGCTCCAAC 
AGCTCTGCCC GATCTAAGCG CAACGCCGCC ACCCGTGACA CCACAGCTGC CGTCGGACTT 
ATAATGAACC ACGCTGTTCT TCATGACAAG GACAGCGGTC AGGCAGACTT GGAGGAAATG 
GATACACAAG AAGACGAAAA CGTGGCCCCT CCACCGTCGC AGCAGCAGCA GCAGCCGCCA 
CAGCCGACCC AGCAGGCCCA GCCCCTGCAC CACCCGCCGC CCCCTCCCCC GCCTGAGACG 
GGCGGGCGGG AGCGCTTTGC CAACGGCGTG TCCCCCGCGG TGGGCGACGT GCACAGCCCG 
CCCCCTCCCG CTCCTCCCGC CTGCGACGAG CCCATGGCGC CGGCCCCGCC CCCCGCCGCC 
GACGCAGGCG CGCTGACGAA GGTGCCGGCG GCAGCAGCGG CGCCGGATGG CCTCGACGAG 
CCCATGGAGG AGGAGGACGA GGCCCGGTCG GAGGCCACCT TCCGCTTTGT GGTGACGGGC 
TTCTCGAGGC TGCGGGAGTC GGTGCTCAGT CCGGCCACCC AAGTGCGCAA CCTCCCCTGG 
AAAATCATGG TGATGCCGCG CACGAATGCG GGCAACGACC GGACACCGAC CAAGAGTCTG 
GGCTTCTTCC TACAATGCAA CGGCGAGTCG GAGTCCTCGA CGTGGTCATG CAACGCGACG 
GCGGAGCTTC GCATCATCAG CCAGAAGGAG GGCGTCGACA ACTTCGTAAG AAAAATCCAG 
CACCTGTTCT ATAGCAAGGA GAACGACTGG GGATTCAGTC ACTTCATGAC GTGGACGGAC 
GTTCTCGATC CGGAGAAAGG GTTCATTCTG GACGACACCA TCATCCTGGA GGTGTGGGTG 
TCGGCGGACG CGCCTCACGG GGTCAGCTGG GACTCCAAAA AGCACACGGG CTACGTGGGC 
CTGAAGAACC AGGGTGCCAC GTGCTACATG AACTCCCTCC TGCAGACACT CTTCTTCACG 
AATCAGCTCC GCAAGGCGGT GTACCAGATG CCGACGGAGA GCGACGACTC GTCCAAGAGC 
GTGGCGCTGG CGCTACAGCG GGTCTTCTAC GAGCTGCAGT TCTCGGACAA GCCCGTGGGC 
ACCAAGAAGC TCACGAAGTC CTTCGGCTGG GAGACGCTCG ACTCTTTTAT GCAGCACGAC 
GTCCAGGAGC TGTGTAGAGT GCTGCTGGAC AACATGGAGA GCAAGATGAA GGGCACCTGC 
GTGGAGGGCA CCATCCCCCG GCTCTTTGAG GGCAAGATGA TCTCGTTCAT CAAGTGCAAG 
CACGTGGAGT ACACCTCCAG GCGCATGGAG CCCTTCTACG ACATCCAGCT GAACGTCAAG 
GGCAAGAAGA ACATCCACGA GTCCTTCCAG GACTACTGCG CCACAGAGAG CCTGGACGGG 
GAGAACAAGT ACGACGCAGG GGAGTACGGG CTGCAGGAGG CGGAGAAAGG CATCATTTTC 
GCCTGCCTGC CCCCCGTGGT GCACCTGCAC CTGCTGCGCT TCCAGTACGA CCCCCTCACG 
GACAACAACG TCAAGCTCAA CGACCGCTTT GAGTTTCCCG AGAAGCTCAA CCTCTCAGAC 
TTCCTCCACG AGAAGGGCCC GAGCCCGGCG ACGTACACCC TGCACGCGGT GCTGGTGCAC 
TCCGGGGACA ACCACGGGGG CCACTACGTG GTGTTCATCA ACCCCCGGGG GGACGGGCGC 
TGGTGCAAGT TCGACGACGA CGTGGTCTCG CGCTGCACCA AGCAGGAGGC CATCGACCAC 
AACTTCGGGG GCACGGAGGA CGACGTGGCC GTCAGCCGCC ACTGCACCAA TGCCTACATG 
CTGGTGTACA TCAGGGACAG CGCCATCAAC GAGGTCCTGC AGTCGGTGAC GGAAAACGAC 
ATCCCCGAGC AGCTGGTCGA GAGGCTTCAG GAGGAAAAGC GCCAGGAGGC CCTGCGGCGC 
AAGGAGCGCA ACGAGGCGCA CCTGTACATG AGCGTGCAGG TGCTGACGGA GGATAGCTTT 
GCGGGACACC AGGGCAACGA CCTGTACGAC CCGGAGAAGG CCTCTTACCG CTCCTTCAAG 
GTGAAGAAGC AAGCCACCCT GCGGGACCTG CTGGACCTGA TTGCAGACCA GATGCGCTTC 
CCCCTGCACG CCATCCGGCC CTGGTCCATC ACCTACCGCT CCAACCAAAC CTTCCGGCCG 
GCCGCCGTGG ACCTTGACGC GGACCTCAAC AAGACGGTGA TTGACCTGTC CGAGAATGCC 
AACCCCTGGA CGATCTTCCT GGAGACGATC GTGCCGGACC AGCCGATGGA CCGGTTGCCG 
GAGTTCGACA AAGAGACGGA CGTGCTGCTC TTCTTCAAGA TGTACGACCC AAGGCTGAAG 
CAGATCGCCT ACTGCGGCCA CACCTACATG CCCATCACCG TCAAGGCCAA GGAGCTGGTG 
CCGCTGCTCA ACAAGAGGGC GGGGTTTCCT CCTGACACGG AGCTGGTGCT CTACGAGGAG 
GTGAAGCCAA ACATTGTGGA CCGCATTGAG GACCTGGAGC TGCCCCTGGA GAAGGTCCTG 
GAGGAGCTGA TGGACGGAGA CATAATCGTC TTTCAGAAGC TGGACCACAA TGCGGACGAG 
TACGAGCTGC CCACCGTCAA GGACTACTTC AGAGACCTCT TCTACAGGGT GGAGGTGACC 
TTCTGTGATA AATCTATTGC CAATGACCCC GGCTTCACGA TGGAGCTTTC TCTCAAGATG 
AACTATGACC AGATCGCCAG GGCGGTGGCG CAACGGCTAG GCACAGACCC TTATTTATTA 
CAATTTTTCA AAGTTCAGAG TTACCGAGAG GGTCCCGGTA ACCCTCTGAG GTGTACATAC 
GAGGGGACGC TCAAGGACCT CCTGGTATTC TTCAAGCCCA GGCAGCCCAA GAAGATCTAT 
TACCAGCAGC TGAGCATACG GATAAACGAG CTAGAAAGCA AGAAGCAGTT CAAGTGCGTC 
TGGGTCAACA GCAAGTTCAA GGAAGAGAAG GAGCTTGTGC TGTACCCGAA CAAGAATGGC 
TGCGTGAGCG ACCTCCTGGA TGAGGCACGC AAGCAGGTGG AGCTCTCGGA CGACGGCACA 
GGTTCTGGCA GGCTACGACT GATGGAGGTG ATCAGCTACA AGATCTTCTC GGTGCCCAAG 
GAGGACGTGC AGCTGGACAC ACTGAACCAG GCCAACTCGA AGACCTACCG GGTGGAGGAG 
ATCCCCCGGG AAGACCTGCA GACCGCGGAC GACGAGATCC TGGTGCCATG CGCCCACTTC 
CAGAAAGAGA TCTTCTCTAC CTTCGGGGTG CCCTTCTTCC TCAAGATGAA GCACCGGGAA 
CCCTTCACGA AGGTCAAGGA CAGGATTCAG AAGAAGCTGG ACATCCCTGA CAAGGAGTTT 
GAAAAGTACA AGTTCGCCAT TGTCGTGATG GGGCGGGCGC AGTTCATCGA GGATGCAGAC 
TACGTGGTGA ACCTGCAAGA CTTCATGGCG CAACCTCCTC AAGGTGCGGC GATGCACCCC 
CGTCCCTGGC TGGGCATCGA ACACATCAAC AAGGCGCCCA AGAGGTCCCG CTACAACTAC 
CTGGAGAAGG CCATCAAGAT CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8024169 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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