LOC8023711 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8023711
Entrez Gene ID	8023711
Full Name	DNA-directed RNA polymerase I largest subunit, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002402529.1	XP_002402573.1	DNA-directed RNA polymerase I largest subunit, putative
XM_029994450.1	XP_029850310.1	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems
Gene Ontology

The following LOC8023711 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8023711 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIa35411	XM_029994450.1 Latest version!	Ixodes scapularis DNA-directed RNA polymerase I subunit RPA1 (LOC8023711), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8023711 ( XM_029994450.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029850310.1 Ixodes scapularis DNA-directed RNA polymerase I subunit RPA1 (LOC8023711), mRNA.

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CloneID	OIa35411
Clone ID Related Accession (Same CDS sequence)	XM_029994450.1
Accession Version	XM_029994450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922374.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCG ACAAGATACC GTATCGCTCC GTCGACGGCG TGAGTTTCAA CGTGTTCAGC 
ACGGACGAAC TTTTGCGACT GAGCGTGCTA AGCGTGACAT GCTCGGAAAC CTTCGACATC 
GTCGGCCACC CCACGAAAGG TGGCCTGCAC GACTCCCTGC TTGGGCCCAG TGACCGAGAT 
GAGGTGTGTG CCACGTGCGG CCTCGGCTTC GTCGCCTGTC CCGGCCACAT TGGGCACATC 
AGCCTCCCAC TGCTCACCTT CAATCCCCTC TTCTTCAGGA CGCTGTACCA GTTGCTGCGC 
GGCAGCTGCT TTGGCTGTGC CCGCCTGCTG TCGCCACCAG CGGTGTGTCA GCTGACTTTG 
GCCCAGTTGC AAGTGCTGCA GTACGGGGCC GTGGGCGCGG TGCAAGAACT GCAGGAGCTT 
GCAGCCGAGG CCATCACGGA CGAGAAGGGC AAGGCCAGCC GCTCTGTCAT GGGCCTGGCG 
CTCAAGAAGC GCATCGACGA GCGCGTCAAG TCCATCATGG AAGGCGTGGA TCTGGAGGAG 
GCGCGGCGCA GCGCCAATGT GAAGAACGTG GTGGAGTGCC GCCAGCGCCT GATCAAGACG 
TTTGTGCGGG AGCACCTGAT GGGGGCCCCC CGCAAGTGCC CTCACTGCGG TGACGTGCGA 
AGGGGCATGA CTATCCAGAA CAACGCACGG TTCGTCTACA CTGGCCGCGC CCAGGTCGCG 
TCTCGGACTC CCGTCCCCAT CGTCGGCACG TCCGGCAGGA TGAGGCGCGA GTTTGGGGAC 
TTTGGGGACG ATGAGGGGAT GGATATCCCT GAAGAGGAAG AGGAGGTGCC GGTGAAGGAG 
GAGTCTGTGG ACACGGGGGC ACAGACGTAC CTGACGCCCC TGGAGGCCCG CAAACACCTG 
CGGCTGCTCT GGCAAAACGA GAGTCCGCTG CTCAGGCACC TGTTTGGAGC CCTGGGCTCG 
GACTCCCAAA AGCATCCCGT GGACATGTTC TTTCTCGAGG CCCTCGTGGT GCCGCCCACG 
CGCTTCCGGC CGATGAACTT CATGCATGGC CGCAAGTATG AGCACCCGCA GACGGTGAGC 
CTGTCCAAGG TTCTGCAGTG TTGCGACGCT GCCAGGGCAG TGCTCAAGCT CATGGAGCGC 
AACCAAGACA GCGAATCCTC TGAGGATACC ATGGAGATGC AGAGATTGCA GCAAGTCGTG 
GACAAGCTAC CAGGCAAGGA CCTGAGTTCT AAGCTTCACG TCGCGTGGAA CAACCTGCAG 
GTTGCGGTTA ACGTCGTGGT GGACAGCGAA CTCGACAGGC TGAGCAGTAA CAAGGCGACG 
CCGGGCATCA AGCAGATCCT GGAGAAGAAA GAGGGCCTGT TCCGCAAGCA CATGATGGGC 
AAGCGTGTGA ACTACGCGGC CCGTTCGGTC ATCTCGCCCG ACCCGTACAT CGGCGTGGAC 
GAGATCGGAG TGCCGATGGT GTTTGCGACG AGGCTTTCTT ACCCTGAGCC TGTCACCGAC 
AGAAATGTGT CAGAGCTCCG AAAGGCCGTC ATCAACGGAC CCAACGTCTA CCCGGGAGCG 
TTGCTTGTGG AGCACGGGGA CGGGACGGTG ACGCGGCTGG ACCCAACAAA CCGTGTCCGT 
CGGGAGGCCA TCGCCAAGCA GCTGATGACG CCGTCGGACT CTTTCAAGAA CACCCTGGCA 
CGGTGCAAGA CGGTGCACCG CCACCTGCGG ACGGGCGACA TGCTGCTGCT AAACCGGCAG 
CCCACGCTAC ACAAGCCGAG TATCATGGCT CACCAGGCAC GAGTGCTGCC CGGTGAAAAG 
ACACTGCGTT TGCACTACGC CAACTGCAAA TGTTACAACG CCGACTTCGA CGGCGACGAG 
ATGAACGCCC ACTTTCCCCA GAGCGAGCTG GCACGTGCAG AGGCCAGTCA GGTGGCGACA 
GTAAACCGGC AGTACCTGGT GCCCAAAGAT GGCACTCCCC TGAGTGGACT CATCCAGGAT 
CATGTGATTG CGGGCTCTTT GCTCACCATG CAGGGGCGCT TTTTCAACAG GGAAGACTAC 
AACCAGCTTG TGTATGGTGC ACTGACGTTT GTACCATCGT CTATCAAGGT GCTTCCTCCG 
GCAATTCAGA AACCAGAGAC CCTTTGGACT GGAAAGCAGG TGCTTTCCAC TGTACTCATC 
AACTGCATTC CTCGGAACAA ACCGGCCCCG ACCGTTGAAG GCAAGGCCAA GATCCCTGCC 
AAGGCGTGGC TGAGGCCGGG TTCGGAAGCG AACGAAGCAG AGGCACAGAT GAGCGAGGGG 
CAGGTGGTGA TCCGGGACGG AGAGCTGCTC TGTGGTGTTC TGGACAAGGC CCACTACGGT 
CCGACCCAGT TTGGGCTGGT CCACGTCTGC TACGAGTTGT ATGGAGGTGC CATTTCCAGT 
CTTGTCCTGA CCGCTTTTGC AAGGCTGTTC ACCCACTTCC TCCAAACCCA CGTCGGGTTT 
ACGCTCGGCA TAGAAGACAT CTTGGTCACC CTCAAGGCCG ACAAGAAACG CAAGAAGATT 
ATGAAGATGG CGAGGAAGGA AGGAGATGGG GCAGCCAAGA AGGCCCTGGG AATTGAGGAG 
GACATTGCGC CCGAGGACCT GGTAGAATCT CTGCGGGGAG CGCACCTCTC CCGCAACGAC 
CGCTACCTTA AGCAGCTGGA TGCCAGCATG AAGTCTGTCA CGGACGACGT CAACAATCAG 
ATCAACAAGG CCTGTATCCC AGATGGTCTC CTGAAGAAGT TTCCAGAAAA CAACCTCCAG 
TTGATGGTCC AGTCAGGAGC TAAGGGCGGC ATGGTGAACT GCATGCAGAT ATCATGCCTG 
CTTGGCCAGA TTGAGCTGGA AGGGCGGAGA GTGCCGCTGA TGCTGAGTGG GCGGACCCTG 
CCCTCCTTTC TGCCCTACGA CACCAGCCCC CGGGCAGGGG GATTCGTGGA CGGGCGCTTC 
CTGACGGGGA TCCGTCCGCA GGAGTTCTTC TTCCACTGCA TGGCTGGCAG GGAGGGTCTC 
GTGGACACGG CAGTGAAGAC CAGCCGCAGT GGGTACCTCC AGCGGTGCCT GATCAAACAC 
CTGGAGGGGC TGATGGTCGG CTACGACCAG ACGGTCAGGG ACAGCGACGG CAGCGTCATC 
CAGTTCCAGT ACGGAGAAGA CGGTCTGGAC GTGCTGAAGA TGCAAATGCT GAAGCCGAGT 
CAGTTCCCGG TGTTGATCAA GAATGCGGAA GCCTTGCTTG ACAGCAAGGA GCTTGAGCAG 
TGTCAGAGCT TCACAGACCA CGAGGAACTG CACAGCGCAA AAAAAGCGGT GAAAAAGTGG 
AAAAAGAAGA CGGATGGGAG GCAAGAAGAG AAGGAGCACC GCGAGAATGC CTTCACCTTC 
TTCTCAAGCC GCGAGGGCGG GGAGTACCGC CGGTTGTATC CCGGGACGAA CCCCGACACC 
GGGCGGCAGC TGCACTGCGA GAAACTGGTC GAGGCGTTCC AGGCGCTCGA GCACAAGTCC 
CTGAAAAAGC TCAACAAGGC CATTGTGAAG CCGGCCGACC CTGTGTGCTC AGTCGTGCGC 
AACGACCTCA ACCTGGGGGC GATCTCTGAG CGGTTGGACG CCCTTGTCGA GGACTACATC 
AGCAAGAACC CGCACAGGCT GCTTGTAGAT CCCAAGCGGT CCCCGGACGG CTCCGTGACG 
GAGCGCGAGA GGAAGGTCCT GTCTCCCGAC GACTTCCGGA ACGTGGTGTA CCTTCGCAAC 
CTGAGGTCCA TCTGCGACCC AGGCGAGCCA GTTGGCCTCT TGGCCGCCCA GAGCATTGGG 
GAGCCGTCCA CGCAGATGAC ACTCAACACC TTCCACTTTG CGGGGAGGGG CGAGATGAAC 
GTGACCCTGG GGATCCCTCG GATGCGGGAG ATACTCATGG TGGCCAGCGC CAACATCGCC 
ACCCCGACCA TGGACCTGCA CCTGCTCCCC GCACCGGACG CCGAGCAGCG AGCCGATCGG 
CTCAGGGTGC TCATCACCGC CGTCAACCTG GCCCAGGTTC TCGAAGAAGT CACTGTCAGA 
GAGAGACTCG TGGTCAAGGG GAGGATGGAA CGGTTCCGGG AGTACCGGGT GCACTTCCAG 
TTCCTTCCGC GCAAGGCGTA CCGCAAGACG CTGCACGCGA CGCCGCAGCG CATCTTGCGC 
TACATGGAGA CCACCTTCCT CCGGCGTCTC GTGGAGGCTG TCAAGAGGAA GCTGGAACAG 
GTCAAGTCCG CCAAGGTCAT CCGGACTACA AGGTCTCGTG ACGGAAGGGC GGGCGGTTCA 
TCGGCTTCCA ACAACGACCA GGGCAATGAC GCCGCCTTCG ACGAGGGATC TGGGGCGGCA 
AGAAACGCGG CCGATGCGGA CGACGACGGG GGCGGGAGCT CCGACGAAGG GGAGGGCGAC 
GGGGACACGG TTGCGCAGCA GGCGCAGTCG AGACACGCCC AGGACCGAGA GTACGAAGAG 
GCGGAAGAGG AAGAACTGCC CGCTGCCCTC TCAGATGAAG AGGTCGATGG CGGAGAGGAA 
GGAAACCTCG CCACAGTCCC AAAACTGGAG AATGCCGAGG AAGACGGAGC GGCGGACGAT 
GAGGGTGACG AACAGAGGTT GGCCTCTCTG AAAAAGGGTG CCCACAAGGT CAAACACCAG 
AAGGCGTCGA GAACGGCGAA GGAGGCGCGA GTCTTTGCCG TGACATCGTT CAACCAATGG 
GTCACGGGTT ACGACTACGA CGTGGATCAA GAAGAGTGGT GCTGCTACAC CTTCAGGCTC 
AGTTTAGAAG GCAGCAAGTT CGACTTCACC TCGGTGGTGG AGGAAGAGTC TGCCAAGGCC 
GTCTTCCATT CGGTACCGGG CATCTCGAGG GCCTTTCTGG TCAAAGACAC CAAGGACCGT 
TCGGGCTGCG GCAAGATGCT CCAGACCGAA GGCGTCAATT TCGTGGAGAT GTTTCGCCAC 
GGGGACATCG TCGACATGCG GCGCATATAC AGCAACGACA TTCACGCCAT CGCCAATACC 
TTTGGGATCG AAGCGGCCAG GACTGCCATC GCGAGGGAGG TGGCCAACGT GTTCGCGGTG 
TACGGCATCG AGGTGGATCC CCGTCACCTG CTCCTGGTGG CCGACTACAT GACCTTCGAC 
GGGACGTACC GGGCCTGCAA CCGCATCTCG ATGGAGAGCA ACGCGTCGCC CCTGCAACAG 
ATGACCTTCG AGACCACCAT GAACTTCCTC AAGTCCGCCA CGCTCTCCGG TATGGAGGAT 
AGGCTCAAGT CTCCGTCTGC TCGTCTTGTG CTGGGAAGGG AAGTGTCTCT CGGTACGGGC 
TGTATGGACC TGAGGCAACC CCACGTGATC TCTCGGAAAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029850310.1
CDS	89..5413
Translation	MDFDKIPYRSVDGVSFNVFSTDELLRLSVLSVTCSETFDIVGHPTKGGLHDSLLGPSDRDEVCATCGLGFVACPGHIGHISLPLLTFNPLFFRTLYQLLRGSCFGCARLLSPPAVCQLTLAQLQVLQYGAVGAVQELQELAAEAITDEKGKASRSVMGLALKKRIDERVKSIMEGVDLEEARRSANVKNVVECRQRLIKTFVREHLMGAPRKCPHCGDVRRGMTIQNNARFVYTGRAQVASRTPVPIVGTSGRMRREFGDFGDDEGMDIPEEEEEVPVKEESVDTGAQTYLTPLEARKHLRLLWQNESPLLRHLFGALGSDSQKHPVDMFFLEALVVPPTRFRPMNFMHGRKYEHPQTVSLSKVLQCCDAARAVLKLMERNQDSESSEDTMEMQRLQQVVDKLPGKDLSSKLHVAWNNLQVAVNVVVDSELDRLSSNKATPGIKQILEKKEGLFRKHMMGKRVNYAARSVISPDPYIGVDEIGVPMVFATRLSYPEPVTDRNVSELRKAVINGPNVYPGALLVEHGDGTVTRLDPTNRVRREAIAKQLMTPSDSFKNTLARCKTVHRHLRTGDMLLLNRQPTLHKPSIMAHQARVLPGEKTLRLHYANCKCYNADFDGDEMNAHFPQSELARAEASQVATVNRQYLVPKDGTPLSGLIQDHVIAGSLLTMQGRFFNREDYNQLVYGALTFVPSSIKVLPPAIQKPETLWTGKQVLSTVLINCIPRNKPAPTVEGKAKIPAKAWLRPGSEANEAEAQMSEGQVVIRDGELLCGVLDKAHYGPTQFGLVHVCYELYGGAISSLVLTAFARLFTHFLQTHVGFTLGIEDILVTLKADKKRKKIMKMARKEGDGAAKKALGIEEDIAPEDLVESLRGAHLSRNDRYLKQLDASMKSVTDDVNNQINKACIPDGLLKKFPENNLQLMVQSGAKGGMVNCMQISCLLGQIELEGRRVPLMLSGRTLPSFLPYDTSPRAGGFVDGRFLTGIRPQEFFFHCMAGREGLVDTAVKTSRSGYLQRCLIKHLEGLMVGYDQTVRDSDGSVIQFQYGEDGLDVLKMQMLKPSQFPVLIKNAEALLDSKELEQCQSFTDHEELHSAKKAVKKWKKKTDGRQEEKEHRENAFTFFSSREGGEYRRLYPGTNPDTGRQLHCEKLVEAFQALEHKSLKKLNKAIVKPADPVCSVVRNDLNLGAISERLDALVEDYISKNPHRLLVDPKRSPDGSVTERERKVLSPDDFRNVVYLRNLRSICDPGEPVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRMREILMVASANIATPTMDLHLLPAPDAEQRADRLRVLITAVNLAQVLEEVTVRERLVVKGRMERFREYRVHFQFLPRKAYRKTLHATPQRILRYMETTFLRRLVEAVKRKLEQVKSAKVIRTTRSRDGRAGGSSASNNDQGNDAAFDEGSGAARNAADADDDGGGSSDEGEGDGDTVAQQAQSRHAQDREYEEAEEEELPAALSDEEVDGGEEGNLATVPKLENAEEDGAADDEGDEQRLASLKKGAHKVKHQKASRTAKEARVFAVTSFNQWVTGYDYDVDQEEWCCYTFRLSLEGSKFDFTSVVEEESAKAVFHSVPGISRAFLVKDTKDRSGCGKMLQTEGVNFVEMFRHGDIVDMRRIYSNDIHAIANTFGIEAARTAIAREVANVFAVYGIEVDPRHLLLVADYMTFDGTYRACNRISMESNASPLQQMTFETTMNFLKSATLSGMEDRLKSPSARLVLGREVSLGTGCMDLRQPHVISRKR

Target ORF information:

RefSeq Version	XM_029994450.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis DNA-directed RNA polymerase I subunit RPA1 (LOC8023711), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029994450.1

ORF Insert Sequence:

ATGGATTTCG ACAAGATACC GTATCGCTCC GTCGACGGCG TGAGTTTCAA CGTGTTCAGC 
ACGGACGAAC TTTTGCGACT GAGCGTGCTA AGCGTGACAT GCTCGGAAAC CTTCGACATC 
GTCGGCCACC CCACGAAAGG TGGCCTGCAC GACTCCCTGC TTGGGCCCAG TGACCGAGAT 
GAGGTGTGTG CCACGTGCGG CCTCGGCTTC GTCGCCTGTC CCGGCCACAT TGGGCACATC 
AGCCTCCCAC TGCTCACCTT CAATCCCCTC TTCTTCAGGA CGCTGTACCA GTTGCTGCGC 
GGCAGCTGCT TTGGCTGTGC CCGCCTGCTG TCGCCACCAG CGGTGTGTCA GCTGACTTTG 
GCCCAGTTGC AAGTGCTGCA GTACGGGGCC GTGGGCGCGG TGCAAGAACT GCAGGAGCTT 
GCAGCCGAGG CCATCACGGA CGAGAAGGGC AAGGCCAGCC GCTCTGTCAT GGGCCTGGCG 
CTCAAGAAGC GCATCGACGA GCGCGTCAAG TCCATCATGG AAGGCGTGGA TCTGGAGGAG 
GCGCGGCGCA GCGCCAATGT GAAGAACGTG GTGGAGTGCC GCCAGCGCCT GATCAAGACG 
TTTGTGCGGG AGCACCTGAT GGGGGCCCCC CGCAAGTGCC CTCACTGCGG TGACGTGCGA 
AGGGGCATGA CTATCCAGAA CAACGCACGG TTCGTCTACA CTGGCCGCGC CCAGGTCGCG 
TCTCGGACTC CCGTCCCCAT CGTCGGCACG TCCGGCAGGA TGAGGCGCGA GTTTGGGGAC 
TTTGGGGACG ATGAGGGGAT GGATATCCCT GAAGAGGAAG AGGAGGTGCC GGTGAAGGAG 
GAGTCTGTGG ACACGGGGGC ACAGACGTAC CTGACGCCCC TGGAGGCCCG CAAACACCTG 
CGGCTGCTCT GGCAAAACGA GAGTCCGCTG CTCAGGCACC TGTTTGGAGC CCTGGGCTCG 
GACTCCCAAA AGCATCCCGT GGACATGTTC TTTCTCGAGG CCCTCGTGGT GCCGCCCACG 
CGCTTCCGGC CGATGAACTT CATGCATGGC CGCAAGTATG AGCACCCGCA GACGGTGAGC 
CTGTCCAAGG TTCTGCAGTG TTGCGACGCT GCCAGGGCAG TGCTCAAGCT CATGGAGCGC 
AACCAAGACA GCGAATCCTC TGAGGATACC ATGGAGATGC AGAGATTGCA GCAAGTCGTG 
GACAAGCTAC CAGGCAAGGA CCTGAGTTCT AAGCTTCACG TCGCGTGGAA CAACCTGCAG 
GTTGCGGTTA ACGTCGTGGT GGACAGCGAA CTCGACAGGC TGAGCAGTAA CAAGGCGACG 
CCGGGCATCA AGCAGATCCT GGAGAAGAAA GAGGGCCTGT TCCGCAAGCA CATGATGGGC 
AAGCGTGTGA ACTACGCGGC CCGTTCGGTC ATCTCGCCCG ACCCGTACAT CGGCGTGGAC 
GAGATCGGAG TGCCGATGGT GTTTGCGACG AGGCTTTCTT ACCCTGAGCC TGTCACCGAC 
AGAAATGTGT CAGAGCTCCG AAAGGCCGTC ATCAACGGAC CCAACGTCTA CCCGGGAGCG 
TTGCTTGTGG AGCACGGGGA CGGGACGGTG ACGCGGCTGG ACCCAACAAA CCGTGTCCGT 
CGGGAGGCCA TCGCCAAGCA GCTGATGACG CCGTCGGACT CTTTCAAGAA CACCCTGGCA 
CGGTGCAAGA CGGTGCACCG CCACCTGCGG ACGGGCGACA TGCTGCTGCT AAACCGGCAG 
CCCACGCTAC ACAAGCCGAG TATCATGGCT CACCAGGCAC GAGTGCTGCC CGGTGAAAAG 
ACACTGCGTT TGCACTACGC CAACTGCAAA TGTTACAACG CCGACTTCGA CGGCGACGAG 
ATGAACGCCC ACTTTCCCCA GAGCGAGCTG GCACGTGCAG AGGCCAGTCA GGTGGCGACA 
GTAAACCGGC AGTACCTGGT GCCCAAAGAT GGCACTCCCC TGAGTGGACT CATCCAGGAT 
CATGTGATTG CGGGCTCTTT GCTCACCATG CAGGGGCGCT TTTTCAACAG GGAAGACTAC 
AACCAGCTTG TGTATGGTGC ACTGACGTTT GTACCATCGT CTATCAAGGT GCTTCCTCCG 
GCAATTCAGA AACCAGAGAC CCTTTGGACT GGAAAGCAGG TGCTTTCCAC TGTACTCATC 
AACTGCATTC CTCGGAACAA ACCGGCCCCG ACCGTTGAAG GCAAGGCCAA GATCCCTGCC 
AAGGCGTGGC TGAGGCCGGG TTCGGAAGCG AACGAAGCAG AGGCACAGAT GAGCGAGGGG 
CAGGTGGTGA TCCGGGACGG AGAGCTGCTC TGTGGTGTTC TGGACAAGGC CCACTACGGT 
CCGACCCAGT TTGGGCTGGT CCACGTCTGC TACGAGTTGT ATGGAGGTGC CATTTCCAGT 
CTTGTCCTGA CCGCTTTTGC AAGGCTGTTC ACCCACTTCC TCCAAACCCA CGTCGGGTTT 
ACGCTCGGCA TAGAAGACAT CTTGGTCACC CTCAAGGCCG ACAAGAAACG CAAGAAGATT 
ATGAAGATGG CGAGGAAGGA AGGAGATGGG GCAGCCAAGA AGGCCCTGGG AATTGAGGAG 
GACATTGCGC CCGAGGACCT GGTAGAATCT CTGCGGGGAG CGCACCTCTC CCGCAACGAC 
CGCTACCTTA AGCAGCTGGA TGCCAGCATG AAGTCTGTCA CGGACGACGT CAACAATCAG 
ATCAACAAGG CCTGTATCCC AGATGGTCTC CTGAAGAAGT TTCCAGAAAA CAACCTCCAG 
TTGATGGTCC AGTCAGGAGC TAAGGGCGGC ATGGTGAACT GCATGCAGAT ATCATGCCTG 
CTTGGCCAGA TTGAGCTGGA AGGGCGGAGA GTGCCGCTGA TGCTGAGTGG GCGGACCCTG 
CCCTCCTTTC TGCCCTACGA CACCAGCCCC CGGGCAGGGG GATTCGTGGA CGGGCGCTTC 
CTGACGGGGA TCCGTCCGCA GGAGTTCTTC TTCCACTGCA TGGCTGGCAG GGAGGGTCTC 
GTGGACACGG CAGTGAAGAC CAGCCGCAGT GGGTACCTCC AGCGGTGCCT GATCAAACAC 
CTGGAGGGGC TGATGGTCGG CTACGACCAG ACGGTCAGGG ACAGCGACGG CAGCGTCATC 
CAGTTCCAGT ACGGAGAAGA CGGTCTGGAC GTGCTGAAGA TGCAAATGCT GAAGCCGAGT 
CAGTTCCCGG TGTTGATCAA GAATGCGGAA GCCTTGCTTG ACAGCAAGGA GCTTGAGCAG 
TGTCAGAGCT TCACAGACCA CGAGGAACTG CACAGCGCAA AAAAAGCGGT GAAAAAGTGG 
AAAAAGAAGA CGGATGGGAG GCAAGAAGAG AAGGAGCACC GCGAGAATGC CTTCACCTTC 
TTCTCAAGCC GCGAGGGCGG GGAGTACCGC CGGTTGTATC CCGGGACGAA CCCCGACACC 
GGGCGGCAGC TGCACTGCGA GAAACTGGTC GAGGCGTTCC AGGCGCTCGA GCACAAGTCC 
CTGAAAAAGC TCAACAAGGC CATTGTGAAG CCGGCCGACC CTGTGTGCTC AGTCGTGCGC 
AACGACCTCA ACCTGGGGGC GATCTCTGAG CGGTTGGACG CCCTTGTCGA GGACTACATC 
AGCAAGAACC CGCACAGGCT GCTTGTAGAT CCCAAGCGGT CCCCGGACGG CTCCGTGACG 
GAGCGCGAGA GGAAGGTCCT GTCTCCCGAC GACTTCCGGA ACGTGGTGTA CCTTCGCAAC 
CTGAGGTCCA TCTGCGACCC AGGCGAGCCA GTTGGCCTCT TGGCCGCCCA GAGCATTGGG 
GAGCCGTCCA CGCAGATGAC ACTCAACACC TTCCACTTTG CGGGGAGGGG CGAGATGAAC 
GTGACCCTGG GGATCCCTCG GATGCGGGAG ATACTCATGG TGGCCAGCGC CAACATCGCC 
ACCCCGACCA TGGACCTGCA CCTGCTCCCC GCACCGGACG CCGAGCAGCG AGCCGATCGG 
CTCAGGGTGC TCATCACCGC CGTCAACCTG GCCCAGGTTC TCGAAGAAGT CACTGTCAGA 
GAGAGACTCG TGGTCAAGGG GAGGATGGAA CGGTTCCGGG AGTACCGGGT GCACTTCCAG 
TTCCTTCCGC GCAAGGCGTA CCGCAAGACG CTGCACGCGA CGCCGCAGCG CATCTTGCGC 
TACATGGAGA CCACCTTCCT CCGGCGTCTC GTGGAGGCTG TCAAGAGGAA GCTGGAACAG 
GTCAAGTCCG CCAAGGTCAT CCGGACTACA AGGTCTCGTG ACGGAAGGGC GGGCGGTTCA 
TCGGCTTCCA ACAACGACCA GGGCAATGAC GCCGCCTTCG ACGAGGGATC TGGGGCGGCA 
AGAAACGCGG CCGATGCGGA CGACGACGGG GGCGGGAGCT CCGACGAAGG GGAGGGCGAC 
GGGGACACGG TTGCGCAGCA GGCGCAGTCG AGACACGCCC AGGACCGAGA GTACGAAGAG 
GCGGAAGAGG AAGAACTGCC CGCTGCCCTC TCAGATGAAG AGGTCGATGG CGGAGAGGAA 
GGAAACCTCG CCACAGTCCC AAAACTGGAG AATGCCGAGG AAGACGGAGC GGCGGACGAT 
GAGGGTGACG AACAGAGGTT GGCCTCTCTG AAAAAGGGTG CCCACAAGGT CAAACACCAG 
AAGGCGTCGA GAACGGCGAA GGAGGCGCGA GTCTTTGCCG TGACATCGTT CAACCAATGG 
GTCACGGGTT ACGACTACGA CGTGGATCAA GAAGAGTGGT GCTGCTACAC CTTCAGGCTC 
AGTTTAGAAG GCAGCAAGTT CGACTTCACC TCGGTGGTGG AGGAAGAGTC TGCCAAGGCC 
GTCTTCCATT CGGTACCGGG CATCTCGAGG GCCTTTCTGG TCAAAGACAC CAAGGACCGT 
TCGGGCTGCG GCAAGATGCT CCAGACCGAA GGCGTCAATT TCGTGGAGAT GTTTCGCCAC 
GGGGACATCG TCGACATGCG GCGCATATAC AGCAACGACA TTCACGCCAT CGCCAATACC 
TTTGGGATCG AAGCGGCCAG GACTGCCATC GCGAGGGAGG TGGCCAACGT GTTCGCGGTG 
TACGGCATCG AGGTGGATCC CCGTCACCTG CTCCTGGTGG CCGACTACAT GACCTTCGAC 
GGGACGTACC GGGCCTGCAA CCGCATCTCG ATGGAGAGCA ACGCGTCGCC CCTGCAACAG 
ATGACCTTCG AGACCACCAT GAACTTCCTC AAGTCCGCCA CGCTCTCCGG TATGGAGGAT 
AGGCTCAAGT CTCCGTCTGC TCGTCTTGTG CTGGGAAGGG AAGTGTCTCT CGGTACGGGC 
TGTATGGACC TGAGGCAACC CCACGTGATC TCTCGGAAAC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8023711 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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