LOC100214999 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100214999
Entrez Gene ID	100214999
Full Name	abnormal spindle-like microcephaly-associated protein homolog
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_012711406.1	XP_012566860.1	abnormal spindle-like microcephaly-associated protein homolog

Pathways from BioSystems
Gene Ontology

The following LOC100214999 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100214999 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc18315	XM_012711406.1 Latest version!	Hydra vulgaris abnormal spindle-like microcephaly-associated protein homolog (LOC100214999), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100214999 ( XM_012711406.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012566860.1 Hydra vulgaris abnormal spindle-like microcephaly-associated protein homolog (LOC100214999), mRNA.

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CloneID	OHc18315
Clone ID Related Accession (Same CDS sequence)	XM_012711406.1
Accession Version	XM_012711406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433606400000
Organism	Hydra vulgaris
Product	abnormal spindle-like microcephaly-associated protein homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004167122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATTTCTT TAAGTTCTTC AAGAGTTTTT CAAGATGAAA TAAAAGAAAA ATGCGAATCT 
CCAGCTCGAC TTCTAGTGAA AAGTTTTGCT AAATGTCCAC GTTTAAGTTT TAACAAAGTT 
AAAGTAGGTA AAAGTGGAAC ATTGGATCTT CTTATTGAAA ATCCTAACAA CTTTCTTGTT 
TCATTGACTA CAGATCAGTT TTCCGTTGAA ACTTCGTTAG AAATTGTTAT TAGCCATGAT 
GTTTTGTTAA ACAATGACAA AACTGTCACG ATTGTAATAC CAGAAAAGTC GTTTAAAGTA 
TTAAAATTTA AATGGAGTCC GAAGAAAGCA GACAAAATCA GACATACCTT GTTCCTTAGA 
TGGGACGAGG GACCTAAACT TCAGGTAATA TTGCTTGGAA CTGCAGAAGA AATTGGATCA 
ACAAAAATTA ATAATAGCAA GATACCCAAA GTTCTTTGCA ACACTCGCAA CTCTTTAGAA 
GAAAAGAAAT CTAGTTTTAC TCCACCAAAA GTTGAAAAAT TATTAACAAA TATGCAGTTA 
GAAACCTCTG CTCGTCGCAC AACTTTTACA GTTGATAGCA CCAGTCCAAT TAAAAAGGTA 
GTAAAGAGAA TTAATGCTGG TTCTTTGTCA CCATTAAGTT TTGATAAATA TGAGTCTGTC 
CAAAAAGAAA ACAAAGATCC ATTAAAGATT GGAAAGGTTA AAAAACTGCC TCTGAACAAC 
TTGAGCCATA GAAAAATTGA AGACAAAAAA AGAGAAGATT TTATTGACTG GAAGCAAGAA 
AATAAACCAA ATAAATCAAG TTCAGCAAAG ATTAATTTAT TTTTAGAAAA GTCAAATTGT 
ATGGAAAATA TTTCAAGTAA CCCAAAGCAT TCTAATTATT TAAAACGACA ACCTGTTTCT 
AGAACTTTAC CTGTAAAGCC TGTTCAAGGT TTGTCTGTTA GGAAGTTAAA GTTAACAGCT 
TCTTCAAAAG CAGATCTTCC ACGTCACCCA TTACCTTACG CTGCAAAGAA TATGTGTTAT 
GATGAGCGAT GGATAGATAA GCAGGAAAGA GGTTTTACCA ATTGGTTAAA TTTTATACTG 
ACTCCCGATT GCAATGACAA TGAGGAAGAT GAAAATGAAC GAAGAGGTAC CTATACAATA 
GATTCAAGTA AAAGACAACC AATTGCTCCT TCGCGTGAAG ATGTCTCTTT TCAAGTGTAT 
TCACTTCGTC ATAAAATGGT TTGTCTTCGC CGTGCAGGTT GTTTGTTATA TCAAAATGAA 
GAATTTTCAT TGATTATACA AAAGATTGAA CGAGAGATTG AATGTAACAT GTTAGCTATC 
CGAGCAGACA AAAGAATTGA AGCAGATTTA GGTATAAAAA CAAAAATCAG AGACATGATT 
CTTTCTTACA ACTCCCTTTG GTTAAGAATT GGTCTTGAGG TCATATATGG GGAGATAATC 
TTATTACAGT CAAACAATGA TGTTTTTAAC TTGTCAAAAT TTGTAATGAG TCGCATGCTT 
GCAAATCCTT TAATATCAAA GCAATACTCT CACACAACTG TTCCCAACTT GTACAGACCA 
GGGTACGAGG AAGCTATTCG TAAATTTATT CTCAAAAAAT TTTTACTTTT GGTTATATTT 
TTGGATCGCG CAAAGCAAAA TCGACTCATA GAGCATGATC CATGTTTATT TTTAAAAAAT 
TCAGAGTTTA AGACAAGTCG TGATCTTTTG CTAGCATTTT CTAGAGATTA TTTGAAAGGC 
GAAGGGGATA TCACAAAGCA TTTGAGCTAC ATGGGTATAG TAGTTCAGCA TCAACAGTCA 
GTTATAGATG AATATGACTT TGGTTTAAAA AATCTTGCTG TTGATTTAAG AGATGGTGTT 
CGTCTAGTGC GTGTTCTTGA ACTTTTAACA AAAGACTGGT CACACTCTTC CAAACTTAGG 
TTACCGGCTG TCAGTAGATT ACAGAAAATT CACAATAATA GTGTTGCCCT TGGAATTTTA 
AAAAATCATG GTTTGAATTT GTCAATAGAA CCTAAAAATG TTGTTGATGG CCACCGTGAG 
AAAACATTAA TGTTACTCTG GCAAATAATA TTTCATTTTG AAGTTAATAT GCTACTTGAT 
ATAGAGAAAC TAAAAGAAGA AATTGAATAT CAAAAAAAAA GGTTGCAAGT AAATAAAGAA 
CTGGCTTCAC TCTCTGAAAG TCTTCCTGAA TCACATGGTG AACTTATATC CGCTTATGAA 
AAGTCAGAAA AAATGACTCT TTTATTAAAT TGGTGTCAAA TTATATGCTC TCATTATGAA 
GTAAAGGTTG AGAATTTTAC AGTTTCATTT TCGGATGGCA TTGCGTTGTG TTGTTTGGTT 
AATTATTACC ACCCAGATAT TCTTCCAACA TCACTTATCA ATCACGAGAC TACACTTACC 
CGTAATATTG CTTTATGTGA AGAGGAAGTT GCTGGTGACT TTTTTTATGA AAACTGGACA 
AAGTGTTTTA GCCCAAATAC TGGTATAAAC AGAAATTTAG AAATGTTGCT TATGAATGAG 
AAACATAATT ACAGAGTGCT TAATCAAGCT GTACAGGATT TGGGTGGCAT TCCTTCTATG 
TTAAGGAGTA GTGATATGTC TAATACTATT CCTGATGAAA AGGTTGTAGT TACATATGTT 
TCTTATTTGT GTGCACGTCT GTTAAATATA AGAGAAGAGA CTCAGGCTGC AAGAAAAATT 
CAGCTATCCT GGAGAAGATA TTCCCTGAGG AAGCAAAGAA ATTTACTTAA GGTTTATGAA 
ACTGCTGCTC TCAAAATTCA GAACTGGTAT CGTAAGCATC ATAACAAACG TCGTAGTGCT 
CAACACACTT ATGCAGTCAA AGTTATTCAA AAGACTTGGA GAAGTTATAA AGAAAGATGT 
TTATGTAATT TTAAATACCA AGCTGAACGT GAAAATAATG CAGCTGTTGT AATACAGAGC 
TATGTACGCT GTTATTTGGC TCGCCTTGAT TATCTTTTTT TACGTGGTTG TATTATACTT 
TGTCAAAGTG TTGCTCGAAG ATGGTTTGTA AAGCAGGATC TTATCAAAAA ACGAATTGCA 
GCCTCCATTA TTCAATCTTG TTTTAGAGGT TATTTACATA CATGTTATTT AAGGTATCAG 
TTTTTGCAAC AAAGAGCCTC ATCAATTGTT ATTCAAAGAG CATTTCGCAA TCATATAAGT 
AGAAAAAAAT CCAAGTTAAA GTATTTTGAG TTATGTAACA GAGCTGCTTC TATTATTCAA 
TCTCACTATA AAGGATTTGT TTGCAAAAAG CAATATAAAT GTCTTAAATC ATCAACTATT 
GTTGTCCAGC AACTAGCTAG AGGTTTAATT CAAAGAAAGA GGTTTTTAAG ACAAAAACAG 
GCTGTTTGTA TAATTGAGAG TTTTTATATT TCCTGCATTA AAAGAAGATT TGCTCAAAGA 
GAATATTTTA CTATCCGAAA ATCTATTTTA AAAGTTCAAC AGCACTGGAG GCAAACTTTG 
TATTCCAGAG TTTTACGATA TAAATACTTG CAAATGAAAT TTGCTGCTAT TCTCATTCAA 
TCGTGGATAA AGATGACACT TGCTCGTAGA AAGTTCTTAA ATATTAAAAA ATCAGTTGTC 
ATTATTGAAA ATGCTTGGTT ATCTTTTCAG CAAGGAAGAT ATGAACAGAG AAAATATTTG 
TGTTTAAAAC ACAGTGTTTA TATAATTGAA TGCAAATATA AAGCTATTCT TTTGATGAGG 
CATGAAAAAG CAAAGTTAAA AAACATTATC CAATCAATTG TTTTAATACA ATCATGTTTC 
AGGTGTTGGT CAATTCGAAA AAAGTTTATA AATAGAAAAA ATGCTTGCAT TCTATTGCAA 
AAAGTTTGGC GTGCGTATTA TGCCAAAAAG CTTGCACATA GAGAGTATTG TAGAAAAAAA 
CATTCTGTTA TACTATTGCA AAAGCAAGTC AGGATGTTTT TAGCAATGAA GGAGTTTTCC 
CTAAAAAAAA AAGCTGTTCT AACAATACAA AGCTTGTTTA GAATGTTTTT GTGTCGTAAA 
AAATATTTAT TGTGCCTCAA AGCTGTTGTT TGTATACAGA GAAATACTAG ACATTATCTT 
TTGAGATATA AAAGAAGAAA AGCAGCTATT AAAATTCAAG CAACACTGAG AATGTTTATT 
TGTTTAAAGA AATTTGCTAA ACAGAAACAC GCAATTCAAG TTATGCAACG CTTTATCCGA 
GTTTTTATAT GTCGACGTGC ATATCAGCAT AAAATTGCTT CTGTAATTAA AATTCAGTCA 
TTTATAAGAA TGTGTATATC AAAGATGTTA TTTAAAAAGC AAAAAAATGC TATCATTAAA 
GTTCAAGCTT TAGCGAGAAG ATACATATGT CAGCAGCTGT TTTTTAAACA TCGTAATGCT 
GCTATTATCA TTCAGTCATT CTTCAGAATG TTACTTATAA AAAAAATCTT TTTGTTAAAG 
ATTAAATCTG CAATACAAAT TCAGTCTTGT TATAGATCAT TCAATGCTTT TAAAAATTAT 
AATATACTAA AGAATTCAGT TCTAAAAATT CAGTCATTCT TTCGGATGTG TATTTGCAAG 
ACTAGATTTA ATGTTCAAAA AAAGCATTCT ATAGTTTTAC AGTCATATAT TCGAATGTTT 
TTGCAAAGAA AGAGATATTT ACAGTTGCAA CATACAGTTA TTGAAGTTCA ATCAAAATGC 
AGAATGAAAA GACAGAGAGA TTTATATCTA AAATTAAGAC AAAGCTCAAT AATAATTCAA 
TCTTTTTTTA GAGGTTACAG AGAAAGACAT TTTTATGCTT TATCAAAAAA GAATATTATC 
AAAATTCAGT CGTATATAAG AATGTTTGTT GCAAGACAAA AGTATATTAG ACAGCGAGTA 
GCCATTATTT TGATTCAGTC TGTTTGCAGA ATGTCTGTTG CGAGGAAAGA TTTCATAAGA 
AAAAAGCTAT CATCTGTCAA AATTCAGACT GCTTATAGAA AGTACATAAT TTATAATAAG 
CAAAAAAAGG CAGCTATAGT AATACAAAAA ATATTTAGAG GAACAATGCA TAGAAAGAAA 
TTTCTTCAAC AAAAGCAAGC AGCTGTACGA TTTCAAGCAT ACTTTAGAGG ATTGATTCAA 
AAAAATAAAT TTATTCGGCA AAAGCAAGCA GCTGTACTAT TGCAAGCATA CTTCAGAGGA 
TTGATTCAAA AAAAGAAATT TCTTCAACAA AAGCAAGCAG CTGTACTATT GCAAGCATAC 
TTTAGAGGAT TGATTCAAAA AAAGAAATTT CTTCAACAAA AGCAAGCAGC TGTACTATTG 
CAAGCATACT TTAGAGGATT GATTCAAAAA AAGAAATTTC TTCAGCAAAA GCAAGCAGCT 
GTACTATTGC AAGCATACTT TAGAGGATTG ATTCAAAAAA AGAAATTTCT TCAACAAAAG 
CAAGCAGCTG TACTATTGCA AGCATTCTTT AGAGGATTGA TTCAAAAAAA GAAATTTCTT 
CAACAAAAGC AAGCAGCTGT ACTATTGCAA GCATACTTTA GAGGATTGAT TCAAAAAAAG 
AAATTTCTTC AACAAAAGCA AGCAGCTGTA CTATTGCAAG CATACTTTAG AGGATTGATT 
CAAAAAAAGA AATTTCTTCA GCAAAGAATT GCTGCTACTC AATTGCAATC TTTTGTGCGA 
ATGTTTTTAC AAAAAAAGCT TTTTAATCAA CAAGTTACAG CTATAAACAA AATTAAAGCT 
TGGTTACAAG GTTATTGCCT TAGACAGCAT TTTAAAAGAC TTAGAAATGC GTGTACTCTA 
TTACAGTCTA TTTATCGAAT GCATTTAAAG AGAAGACATT TTAAAATTCT AAAAAAGTCT 
GCAGTTATTA TTCAATCTAC ATTCAGAAGC CACTACTGTT ATTCAAAGTA TGTATTTTTG 
AAATCTGCGG TTGTTAGAAT TCAAACAAAT TACAGAATGC ATTGCTGCAT TGTGAAATAT 
AAAAGTTTAC GAGTTGCAGT AATCAAAATT CAAGCTTTTG CAAGAATGGT TATATTTAGA 
AGTAACTATA AAAAATTGCA AAAAGAAGTG AAGAAATTGC AATCTTGGTG GAGAATGTGC 
TATTTACAGA TTATTTTAAA AAGACAAACA TCTTCTGCCA TTAAAATCCA ATCTATTTAC 
CGAATGTTTG TTATAAAGAA AACTTATTTA AATAAAAGAA ATGCAGCAAT TAAAATACAG 
GCTTTTGTAA GAATGTTTAT AACTCAAAGT AAATATAAAA AATTGCAACA GTTAAAATCA 
GCCACAACCA TACAATTTTA TTACCGAAGT TGGAAAGTTA GAAATGCTAG GAGAAAATTC 
ATTTTAGAAA ACTGTGTTAC TATACAAAGA TGGTGGCGCA AAAAGAGAGA AAATTTACAT 
CAACATTATG CTGCAGTCAT ATTGCAATCT TGTTTTCGAA TGTTTTTATT AAGACAAGAA 
TATTTAAGAA AGCTAAAAGC AGTTTCAGTC ATCAGAGGTT GGTATCAAAT GCATCGACAA 
CGTTCCGTAT ATTTAAAATT AATTAAATCA GCTATAAAGT TGCAAAGTTG GTATCGTTCA 
GTTTTAGCAC GCAATCGTTT TTTAATCATG AAGCGTGCAG CAATCACTAT TCAGCTATAT 
TACAGAGCAT TTACTGTTGG ATATCCAATA TATTTATATT ATCATGTTTA CCGTGGTGCA 
ATCATAACTA TTCAAGCTGT TTATAGGGGA CATATTGCAA GAAAACAATT TCAAAGAAAA 
AAGAAAAGCA TTGTTGTTTT GCAATCTTTT TTTTTAATGG TAACTCAACG TAAAAGATAT 
TTATATTTTC TATCCACTGT TAAATTTTGT CAAAATAAAA TTAGATCAGT TTTGATAAGA 
AAACATTACT TAAAAATCAA ATCATCAGTT TTAACTATTC AATCTTTTGT TCGTCAGCAT 
ATTGCGCGTT GCAAGTTTTA TGCTGCACTA AAAATTCAAT GTGCTTTTAA ATCATTTCTT 
GCTGTTCAGC ATCTAAAAAC ACTAAAGCAT ATAGACAAGA AAAATAAAGC GGTTCTTTAT 
CTTCAAAGAA CATACAGGAG AAATGCACTA TGCAGAATCG AAAGAAAAAA TTATCTTAGA 
ATTCGAAATT CTACTATAGT CCTACAAAGT CACATTCGAA AGTGGTTAGC TTTTAAACAT 
TATTCAAAGC TGTATAAATC CATATGTTTA ATTCAGCGAG TTTGGAAAAG TTATAATTAT 
ACTAAAAAAA TTTCTATTTG TTGTGAGGAA GTTTTAAATA TTGTTCAAAT TTGTTACAAT 
TATTGTCAAG TTCGCACAAA TGCTTCATTA AAAATAACTT CTTTCATGAA AATGATAGTC 
ATGCAAAAAA AGTATCGCAA AGTAAAGAAT TCTGTACTTT GTATTCAGAA AACATGGCGA 
AAGTTTGCTA TAAAAAAGAA AACAAATGCA GTTAGAAAGA TTGAGGCATG GTATATCCGT 
TCAAAAACAA TTAAAAAAAT GAAAGAATTA CAAAAAGTAG AAAACGCAGC ACTAGTAATA 
CAAAAATCAT ATAGAAACTA TTGTGTTTCT AAAAAAGAAA AACAAAAATA TTTAAGACTA 
AAATCTTCTG TAATACTTTT GCAATGTGTG TTTCGCAAGA AATTAGCTTC CAAAAAATTA 
CTCATTATGA AGCAAGCTGC AAATAAAATC GAAAACTGGT ATTCATTATG CAAGCAGAAA 
CATCAAAAAA AAAGACAATT GATCTTACTA AATTTTATAC GAGCCTCTTT ATTGTGTCTT 
TCATTGCTCA AAATTCAAAG GTGGATGCGA AAAAAATTAA ATAATAAATT TGAAAAAGAA 
CATTCTAAAA AAGAGTTTTC AGCACTTGTT ATACAGTCAC GTTGGCGTGG TTATCGAGTT 
CGTTCTCGCA TTAACGATAG TAGGTTGAAA GAAGTTGCTG TACGAATCAA TATAGCTACA 
AATAATTCAA CTGAAGCTAT GAAATTGTGT AATCGAACTA AATCCGCACT TGACTACATA 
CTTCATGTGA AAAATTTATC AACAGTTCAT GAAGCACTTA AAAATTTAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012566860.1
CDS	62..8095
Translation	MISLSSSRVFQDEIKEKCESPARLLVKSFAKCPRLSFNKVKVGKSGTLDLLIENPNNFLVSLTTDQFSVETSLEIVISHDVLLNNDKTVTIVIPEKSFKVLKFKWSPKKADKIRHTLFLRWDEGPKLQVILLGTAEEIGSTKINNSKIPKVLCNTRNSLEEKKSSFTPPKVEKLLTNMQLETSARRTTFTVDSTSPIKKVVKRINAGSLSPLSFDKYESVQKENKDPLKIGKVKKLPLNNLSHRKIEDKKREDFIDWKQENKPNKSSSAKINLFLEKSNCMENISSNPKHSNYLKRQPVSRTLPVKPVQGLSVRKLKLTASSKADLPRHPLPYAAKNMCYDERWIDKQERGFTNWLNFILTPDCNDNEEDENERRGTYTIDSSKRQPIAPSREDVSFQVYSLRHKMVCLRRAGCLLYQNEEFSLIIQKIEREIECNMLAIRADKRIEADLGIKTKIRDMILSYNSLWLRIGLEVIYGEIILLQSNNDVFNLSKFVMSRMLANPLISKQYSHTTVPNLYRPGYEEAIRKFILKKFLLLVIFLDRAKQNRLIEHDPCLFLKNSEFKTSRDLLLAFSRDYLKGEGDITKHLSYMGIVVQHQQSVIDEYDFGLKNLAVDLRDGVRLVRVLELLTKDWSHSSKLRLPAVSRLQKIHNNSVALGILKNHGLNLSIEPKNVVDGHREKTLMLLWQIIFHFEVNMLLDIEKLKEEIEYQKKRLQVNKELASLSESLPESHGELISAYEKSEKMTLLLNWCQIICSHYEVKVENFTVSFSDGIALCCLVNYYHPDILPTSLINHETTLTRNIALCEEEVAGDFFYENWTKCFSPNTGINRNLEMLLMNEKHNYRVLNQAVQDLGGIPSMLRSSDMSNTIPDEKVVVTYVSYLCARLLNIREETQAARKIQLSWRRYSLRKQRNLLKVYETAALKIQNWYRKHHNKRRSAQHTYAVKVIQKTWRSYKERCLCNFKYQAERENNAAVVIQSYVRCYLARLDYLFLRGCIILCQSVARRWFVKQDLIKKRIAASIIQSCFRGYLHTCYLRYQFLQQRASSIVIQRAFRNHISRKKSKLKYFELCNRAASIIQSHYKGFVCKKQYKCLKSSTIVVQQLARGLIQRKRFLRQKQAVCIIESFYISCIKRRFAQREYFTIRKSILKVQQHWRQTLYSRVLRYKYLQMKFAAILIQSWIKMTLARRKFLNIKKSVVIIENAWLSFQQGRYEQRKYLCLKHSVYIIECKYKAILLMRHEKAKLKNIIQSIVLIQSCFRCWSIRKKFINRKNACILLQKVWRAYYAKKLAHREYCRKKHSVILLQKQVRMFLAMKEFSLKKKAVLTIQSLFRMFLCRKKYLLCLKAVVCIQRNTRHYLLRYKRRKAAIKIQATLRMFICLKKFAKQKHAIQVMQRFIRVFICRRAYQHKIASVIKIQSFIRMCISKMLFKKQKNAIIKVQALARRYICQQLFFKHRNAAIIIQSFFRMLLIKKIFLLKIKSAIQIQSCYRSFNAFKNYNILKNSVLKIQSFFRMCICKTRFNVQKKHSIVLQSYIRMFLQRKRYLQLQHTVIEVQSKCRMKRQRDLYLKLRQSSIIIQSFFRGYRERHFYALSKKNIIKIQSYIRMFVARQKYIRQRVAIILIQSVCRMSVARKDFIRKKLSSVKIQTAYRKYIIYNKQKKAAIVIQKIFRGTMHRKKFLQQKQAAVRFQAYFRGLIQKNKFIRQKQAAVLLQAYFRGLIQKKKFLQQKQAAVLLQAYFRGLIQKKKFLQQKQAAVLLQAYFRGLIQKKKFLQQKQAAVLLQAYFRGLIQKKKFLQQKQAAVLLQAFFRGLIQKKKFLQQKQAAVLLQAYFRGLIQKKKFLQQKQAAVLLQAYFRGLIQKKKFLQQRIAATQLQSFVRMFLQKKLFNQQVTAINKIKAWLQGYCLRQHFKRLRNACTLLQSIYRMHLKRRHFKILKKSAVIIQSTFRSHYCYSKYVFLKSAVVRIQTNYRMHCCIVKYKSLRVAVIKIQAFARMVIFRSNYKKLQKEVKKLQSWWRMCYLQIILKRQTSSAIKIQSIYRMFVIKKTYLNKRNAAIKIQAFVRMFITQSKYKKLQQLKSATTIQFYYRSWKVRNARRKFILENCVTIQRWWRKKRENLHQHYAAVILQSCFRMFLLRQEYLRKLKAVSVIRGWYQMHRQRSVYLKLIKSAIKLQSWYRSVLARNRFLIMKRAAITIQLYYRAFTVGYPIYLYYHVYRGAIITIQAVYRGHIARKQFQRKKKSIVVLQSFFLMVTQRKRYLYFLSTVKFCQNKIRSVLIRKHYLKIKSSVLTIQSFVRQHIARCKFYAALKIQCAFKSFLAVQHLKTLKHIDKKNKAVLYLQRTYRRNALCRIERKNYLRIRNSTIVLQSHIRKWLAFKHYSKLYKSICLIQRVWKSYNYTKKISICCEEVLNIVQICYNYCQVRTNASLKITSFMKMIVMQKKYRKVKNSVLCIQKTWRKFAIKKKTNAVRKIEAWYIRSKTIKKMKELQKVENAALVIQKSYRNYCVSKKEKQKYLRLKSSVILLQCVFRKKLASKKLLIMKQAANKIENWYSLCKQKHQKKRQLILLNFIRASLLCLSLLKIQRWMRKKLNNKFEKEHSKKEFSALVIQSRWRGYRVRSRINDSRLKEVAVRINIATNNSTEAMKLCNRTKSALDYILHVKNLSTVHEALKNLG

Target ORF information:

RefSeq Version	XM_012711406.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris abnormal spindle-like microcephaly-associated protein homolog (LOC100214999), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012711406.1

ORF Insert Sequence:

ATGATTTCTT TAAGTTCTTC AAGAGTTTTT CAAGATGAAA TAAAAGAAAA ATGCGAATCT 
CCAGCTCGAC TTCTAGTGAA AAGTTTTGCT AAATGTCCAC GTTTAAGTTT TAACAAAGTT 
AAAGTAGGTA AAAGTGGAAC ATTGGATCTT CTTATTGAAA ATCCTAACAA CTTTCTTGTT 
TCATTGACTA CAGATCAGTT TTCCGTTGAA ACTTCGTTAG AAATTGTTAT TAGCCATGAT 
GTTTTGTTAA ACAATGACAA AACTGTCACG ATTGTAATAC CAGAAAAGTC GTTTAAAGTA 
TTAAAATTTA AATGGAGTCC GAAGAAAGCA GACAAAATCA GACATACCTT GTTCCTTAGA 
TGGGACGAGG GACCTAAACT TCAGGTAATA TTGCTTGGAA CTGCAGAAGA AATTGGATCA 
ACAAAAATTA ATAATAGCAA GATACCCAAA GTTCTTTGCA ACACTCGCAA CTCTTTAGAA 
GAAAAGAAAT CTAGTTTTAC TCCACCAAAA GTTGAAAAAT TATTAACAAA TATGCAGTTA 
GAAACCTCTG CTCGTCGCAC AACTTTTACA GTTGATAGCA CCAGTCCAAT TAAAAAGGTA 
GTAAAGAGAA TTAATGCTGG TTCTTTGTCA CCATTAAGTT TTGATAAATA TGAGTCTGTC 
CAAAAAGAAA ACAAAGATCC ATTAAAGATT GGAAAGGTTA AAAAACTGCC TCTGAACAAC 
TTGAGCCATA GAAAAATTGA AGACAAAAAA AGAGAAGATT TTATTGACTG GAAGCAAGAA 
AATAAACCAA ATAAATCAAG TTCAGCAAAG ATTAATTTAT TTTTAGAAAA GTCAAATTGT 
ATGGAAAATA TTTCAAGTAA CCCAAAGCAT TCTAATTATT TAAAACGACA ACCTGTTTCT 
AGAACTTTAC CTGTAAAGCC TGTTCAAGGT TTGTCTGTTA GGAAGTTAAA GTTAACAGCT 
TCTTCAAAAG CAGATCTTCC ACGTCACCCA TTACCTTACG CTGCAAAGAA TATGTGTTAT 
GATGAGCGAT GGATAGATAA GCAGGAAAGA GGTTTTACCA ATTGGTTAAA TTTTATACTG 
ACTCCCGATT GCAATGACAA TGAGGAAGAT GAAAATGAAC GAAGAGGTAC CTATACAATA 
GATTCAAGTA AAAGACAACC AATTGCTCCT TCGCGTGAAG ATGTCTCTTT TCAAGTGTAT 
TCACTTCGTC ATAAAATGGT TTGTCTTCGC CGTGCAGGTT GTTTGTTATA TCAAAATGAA 
GAATTTTCAT TGATTATACA AAAGATTGAA CGAGAGATTG AATGTAACAT GTTAGCTATC 
CGAGCAGACA AAAGAATTGA AGCAGATTTA GGTATAAAAA CAAAAATCAG AGACATGATT 
CTTTCTTACA ACTCCCTTTG GTTAAGAATT GGTCTTGAGG TCATATATGG GGAGATAATC 
TTATTACAGT CAAACAATGA TGTTTTTAAC TTGTCAAAAT TTGTAATGAG TCGCATGCTT 
GCAAATCCTT TAATATCAAA GCAATACTCT CACACAACTG TTCCCAACTT GTACAGACCA 
GGGTACGAGG AAGCTATTCG TAAATTTATT CTCAAAAAAT TTTTACTTTT GGTTATATTT 
TTGGATCGCG CAAAGCAAAA TCGACTCATA GAGCATGATC CATGTTTATT TTTAAAAAAT 
TCAGAGTTTA AGACAAGTCG TGATCTTTTG CTAGCATTTT CTAGAGATTA TTTGAAAGGC 
GAAGGGGATA TCACAAAGCA TTTGAGCTAC ATGGGTATAG TAGTTCAGCA TCAACAGTCA 
GTTATAGATG AATATGACTT TGGTTTAAAA AATCTTGCTG TTGATTTAAG AGATGGTGTT 
CGTCTAGTGC GTGTTCTTGA ACTTTTAACA AAAGACTGGT CACACTCTTC CAAACTTAGG 
TTACCGGCTG TCAGTAGATT ACAGAAAATT CACAATAATA GTGTTGCCCT TGGAATTTTA 
AAAAATCATG GTTTGAATTT GTCAATAGAA CCTAAAAATG TTGTTGATGG CCACCGTGAG 
AAAACATTAA TGTTACTCTG GCAAATAATA TTTCATTTTG AAGTTAATAT GCTACTTGAT 
ATAGAGAAAC TAAAAGAAGA AATTGAATAT CAAAAAAAAA GGTTGCAAGT AAATAAAGAA 
CTGGCTTCAC TCTCTGAAAG TCTTCCTGAA TCACATGGTG AACTTATATC CGCTTATGAA 
AAGTCAGAAA AAATGACTCT TTTATTAAAT TGGTGTCAAA TTATATGCTC TCATTATGAA 
GTAAAGGTTG AGAATTTTAC AGTTTCATTT TCGGATGGCA TTGCGTTGTG TTGTTTGGTT 
AATTATTACC ACCCAGATAT TCTTCCAACA TCACTTATCA ATCACGAGAC TACACTTACC 
CGTAATATTG CTTTATGTGA AGAGGAAGTT GCTGGTGACT TTTTTTATGA AAACTGGACA 
AAGTGTTTTA GCCCAAATAC TGGTATAAAC AGAAATTTAG AAATGTTGCT TATGAATGAG 
AAACATAATT ACAGAGTGCT TAATCAAGCT GTACAGGATT TGGGTGGCAT TCCTTCTATG 
TTAAGGAGTA GTGATATGTC TAATACTATT CCTGATGAAA AGGTTGTAGT TACATATGTT 
TCTTATTTGT GTGCACGTCT GTTAAATATA AGAGAAGAGA CTCAGGCTGC AAGAAAAATT 
CAGCTATCCT GGAGAAGATA TTCCCTGAGG AAGCAAAGAA ATTTACTTAA GGTTTATGAA 
ACTGCTGCTC TCAAAATTCA GAACTGGTAT CGTAAGCATC ATAACAAACG TCGTAGTGCT 
CAACACACTT ATGCAGTCAA AGTTATTCAA AAGACTTGGA GAAGTTATAA AGAAAGATGT 
TTATGTAATT TTAAATACCA AGCTGAACGT GAAAATAATG CAGCTGTTGT AATACAGAGC 
TATGTACGCT GTTATTTGGC TCGCCTTGAT TATCTTTTTT TACGTGGTTG TATTATACTT 
TGTCAAAGTG TTGCTCGAAG ATGGTTTGTA AAGCAGGATC TTATCAAAAA ACGAATTGCA 
GCCTCCATTA TTCAATCTTG TTTTAGAGGT TATTTACATA CATGTTATTT AAGGTATCAG 
TTTTTGCAAC AAAGAGCCTC ATCAATTGTT ATTCAAAGAG CATTTCGCAA TCATATAAGT 
AGAAAAAAAT CCAAGTTAAA GTATTTTGAG TTATGTAACA GAGCTGCTTC TATTATTCAA 
TCTCACTATA AAGGATTTGT TTGCAAAAAG CAATATAAAT GTCTTAAATC ATCAACTATT 
GTTGTCCAGC AACTAGCTAG AGGTTTAATT CAAAGAAAGA GGTTTTTAAG ACAAAAACAG 
GCTGTTTGTA TAATTGAGAG TTTTTATATT TCCTGCATTA AAAGAAGATT TGCTCAAAGA 
GAATATTTTA CTATCCGAAA ATCTATTTTA AAAGTTCAAC AGCACTGGAG GCAAACTTTG 
TATTCCAGAG TTTTACGATA TAAATACTTG CAAATGAAAT TTGCTGCTAT TCTCATTCAA 
TCGTGGATAA AGATGACACT TGCTCGTAGA AAGTTCTTAA ATATTAAAAA ATCAGTTGTC 
ATTATTGAAA ATGCTTGGTT ATCTTTTCAG CAAGGAAGAT ATGAACAGAG AAAATATTTG 
TGTTTAAAAC ACAGTGTTTA TATAATTGAA TGCAAATATA AAGCTATTCT TTTGATGAGG 
CATGAAAAAG CAAAGTTAAA AAACATTATC CAATCAATTG TTTTAATACA ATCATGTTTC 
AGGTGTTGGT CAATTCGAAA AAAGTTTATA AATAGAAAAA ATGCTTGCAT TCTATTGCAA 
AAAGTTTGGC GTGCGTATTA TGCCAAAAAG CTTGCACATA GAGAGTATTG TAGAAAAAAA 
CATTCTGTTA TACTATTGCA AAAGCAAGTC AGGATGTTTT TAGCAATGAA GGAGTTTTCC 
CTAAAAAAAA AAGCTGTTCT AACAATACAA AGCTTGTTTA GAATGTTTTT GTGTCGTAAA 
AAATATTTAT TGTGCCTCAA AGCTGTTGTT TGTATACAGA GAAATACTAG ACATTATCTT 
TTGAGATATA AAAGAAGAAA AGCAGCTATT AAAATTCAAG CAACACTGAG AATGTTTATT 
TGTTTAAAGA AATTTGCTAA ACAGAAACAC GCAATTCAAG TTATGCAACG CTTTATCCGA 
GTTTTTATAT GTCGACGTGC ATATCAGCAT AAAATTGCTT CTGTAATTAA AATTCAGTCA 
TTTATAAGAA TGTGTATATC AAAGATGTTA TTTAAAAAGC AAAAAAATGC TATCATTAAA 
GTTCAAGCTT TAGCGAGAAG ATACATATGT CAGCAGCTGT TTTTTAAACA TCGTAATGCT 
GCTATTATCA TTCAGTCATT CTTCAGAATG TTACTTATAA AAAAAATCTT TTTGTTAAAG 
ATTAAATCTG CAATACAAAT TCAGTCTTGT TATAGATCAT TCAATGCTTT TAAAAATTAT 
AATATACTAA AGAATTCAGT TCTAAAAATT CAGTCATTCT TTCGGATGTG TATTTGCAAG 
ACTAGATTTA ATGTTCAAAA AAAGCATTCT ATAGTTTTAC AGTCATATAT TCGAATGTTT 
TTGCAAAGAA AGAGATATTT ACAGTTGCAA CATACAGTTA TTGAAGTTCA ATCAAAATGC 
AGAATGAAAA GACAGAGAGA TTTATATCTA AAATTAAGAC AAAGCTCAAT AATAATTCAA 
TCTTTTTTTA GAGGTTACAG AGAAAGACAT TTTTATGCTT TATCAAAAAA GAATATTATC 
AAAATTCAGT CGTATATAAG AATGTTTGTT GCAAGACAAA AGTATATTAG ACAGCGAGTA 
GCCATTATTT TGATTCAGTC TGTTTGCAGA ATGTCTGTTG CGAGGAAAGA TTTCATAAGA 
AAAAAGCTAT CATCTGTCAA AATTCAGACT GCTTATAGAA AGTACATAAT TTATAATAAG 
CAAAAAAAGG CAGCTATAGT AATACAAAAA ATATTTAGAG GAACAATGCA TAGAAAGAAA 
TTTCTTCAAC AAAAGCAAGC AGCTGTACGA TTTCAAGCAT ACTTTAGAGG ATTGATTCAA 
AAAAATAAAT TTATTCGGCA AAAGCAAGCA GCTGTACTAT TGCAAGCATA CTTCAGAGGA 
TTGATTCAAA AAAAGAAATT TCTTCAACAA AAGCAAGCAG CTGTACTATT GCAAGCATAC 
TTTAGAGGAT TGATTCAAAA AAAGAAATTT CTTCAACAAA AGCAAGCAGC TGTACTATTG 
CAAGCATACT TTAGAGGATT GATTCAAAAA AAGAAATTTC TTCAGCAAAA GCAAGCAGCT 
GTACTATTGC AAGCATACTT TAGAGGATTG ATTCAAAAAA AGAAATTTCT TCAACAAAAG 
CAAGCAGCTG TACTATTGCA AGCATTCTTT AGAGGATTGA TTCAAAAAAA GAAATTTCTT 
CAACAAAAGC AAGCAGCTGT ACTATTGCAA GCATACTTTA GAGGATTGAT TCAAAAAAAG 
AAATTTCTTC AACAAAAGCA AGCAGCTGTA CTATTGCAAG CATACTTTAG AGGATTGATT 
CAAAAAAAGA AATTTCTTCA GCAAAGAATT GCTGCTACTC AATTGCAATC TTTTGTGCGA 
ATGTTTTTAC AAAAAAAGCT TTTTAATCAA CAAGTTACAG CTATAAACAA AATTAAAGCT 
TGGTTACAAG GTTATTGCCT TAGACAGCAT TTTAAAAGAC TTAGAAATGC GTGTACTCTA 
TTACAGTCTA TTTATCGAAT GCATTTAAAG AGAAGACATT TTAAAATTCT AAAAAAGTCT 
GCAGTTATTA TTCAATCTAC ATTCAGAAGC CACTACTGTT ATTCAAAGTA TGTATTTTTG 
AAATCTGCGG TTGTTAGAAT TCAAACAAAT TACAGAATGC ATTGCTGCAT TGTGAAATAT 
AAAAGTTTAC GAGTTGCAGT AATCAAAATT CAAGCTTTTG CAAGAATGGT TATATTTAGA 
AGTAACTATA AAAAATTGCA AAAAGAAGTG AAGAAATTGC AATCTTGGTG GAGAATGTGC 
TATTTACAGA TTATTTTAAA AAGACAAACA TCTTCTGCCA TTAAAATCCA ATCTATTTAC 
CGAATGTTTG TTATAAAGAA AACTTATTTA AATAAAAGAA ATGCAGCAAT TAAAATACAG 
GCTTTTGTAA GAATGTTTAT AACTCAAAGT AAATATAAAA AATTGCAACA GTTAAAATCA 
GCCACAACCA TACAATTTTA TTACCGAAGT TGGAAAGTTA GAAATGCTAG GAGAAAATTC 
ATTTTAGAAA ACTGTGTTAC TATACAAAGA TGGTGGCGCA AAAAGAGAGA AAATTTACAT 
CAACATTATG CTGCAGTCAT ATTGCAATCT TGTTTTCGAA TGTTTTTATT AAGACAAGAA 
TATTTAAGAA AGCTAAAAGC AGTTTCAGTC ATCAGAGGTT GGTATCAAAT GCATCGACAA 
CGTTCCGTAT ATTTAAAATT AATTAAATCA GCTATAAAGT TGCAAAGTTG GTATCGTTCA 
GTTTTAGCAC GCAATCGTTT TTTAATCATG AAGCGTGCAG CAATCACTAT TCAGCTATAT 
TACAGAGCAT TTACTGTTGG ATATCCAATA TATTTATATT ATCATGTTTA CCGTGGTGCA 
ATCATAACTA TTCAAGCTGT TTATAGGGGA CATATTGCAA GAAAACAATT TCAAAGAAAA 
AAGAAAAGCA TTGTTGTTTT GCAATCTTTT TTTTTAATGG TAACTCAACG TAAAAGATAT 
TTATATTTTC TATCCACTGT TAAATTTTGT CAAAATAAAA TTAGATCAGT TTTGATAAGA 
AAACATTACT TAAAAATCAA ATCATCAGTT TTAACTATTC AATCTTTTGT TCGTCAGCAT 
ATTGCGCGTT GCAAGTTTTA TGCTGCACTA AAAATTCAAT GTGCTTTTAA ATCATTTCTT 
GCTGTTCAGC ATCTAAAAAC ACTAAAGCAT ATAGACAAGA AAAATAAAGC GGTTCTTTAT 
CTTCAAAGAA CATACAGGAG AAATGCACTA TGCAGAATCG AAAGAAAAAA TTATCTTAGA 
ATTCGAAATT CTACTATAGT CCTACAAAGT CACATTCGAA AGTGGTTAGC TTTTAAACAT 
TATTCAAAGC TGTATAAATC CATATGTTTA ATTCAGCGAG TTTGGAAAAG TTATAATTAT 
ACTAAAAAAA TTTCTATTTG TTGTGAGGAA GTTTTAAATA TTGTTCAAAT TTGTTACAAT 
TATTGTCAAG TTCGCACAAA TGCTTCATTA AAAATAACTT CTTTCATGAA AATGATAGTC 
ATGCAAAAAA AGTATCGCAA AGTAAAGAAT TCTGTACTTT GTATTCAGAA AACATGGCGA 
AAGTTTGCTA TAAAAAAGAA AACAAATGCA GTTAGAAAGA TTGAGGCATG GTATATCCGT 
TCAAAAACAA TTAAAAAAAT GAAAGAATTA CAAAAAGTAG AAAACGCAGC ACTAGTAATA 
CAAAAATCAT ATAGAAACTA TTGTGTTTCT AAAAAAGAAA AACAAAAATA TTTAAGACTA 
AAATCTTCTG TAATACTTTT GCAATGTGTG TTTCGCAAGA AATTAGCTTC CAAAAAATTA 
CTCATTATGA AGCAAGCTGC AAATAAAATC GAAAACTGGT ATTCATTATG CAAGCAGAAA 
CATCAAAAAA AAAGACAATT GATCTTACTA AATTTTATAC GAGCCTCTTT ATTGTGTCTT 
TCATTGCTCA AAATTCAAAG GTGGATGCGA AAAAAATTAA ATAATAAATT TGAAAAAGAA 
CATTCTAAAA AAGAGTTTTC AGCACTTGTT ATACAGTCAC GTTGGCGTGG TTATCGAGTT 
CGTTCTCGCA TTAACGATAG TAGGTTGAAA GAAGTTGCTG TACGAATCAA TATAGCTACA 
AATAATTCAA CTGAAGCTAT GAAATTGTGT AATCGAACTA AATCCGCACT TGACTACATA 
CTTCATGTGA AAAATTTATC AACAGTTCAT GAAGCACTTA AAAATTTAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100214999 cDNA ORF clone, Hydra vulgaris

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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