| Gene Symbol | RUBCN |
| Entrez Gene ID | 9711 |
| Full Name | RUN and cysteine rich domain containing beclin 1 interacting protein |
| Synonyms | KIAA0226,RUBICON,SCAR15 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]. |
| Disorder MIM: | |
| Disorder Html: | ?Spinocerebellar ataxia, autosomal recessive 15, 615705 (3) |
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RUBCN cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following RUBCN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RUBCN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu27911 Clone ID Related Accession (Same CDS sequence) NM_001145642.4 Accession Version NM_001145642.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1564934400000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB036516.1, D86979.3, AC024560.26 and AI796534.1. This sequence is a reference standard in the RefSeqGene project. On Nov 5, 2016 this sequence version replaced NM_001145642.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86979.3, SRR1803613.27601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
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601
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721
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841
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961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA
GCCGCCGTCC TGGAGGCCAC CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001139114.1 CDS 547..3330 Translation 
Target ORF information:
RefSeq Version NM_001145642.4 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_001145642.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA
GCCGCCGTCC TGGAGGCCAC CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu27916 Clone ID Related Accession (Same CDS sequence) NM_014687.4 , NM_014687.3 Accession Version NM_014687.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1571932800000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 2 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY003277.1, BC033615.1, D86979.3, AC024560.26 and AI796534.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014687.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000296343.10/ ENSP00000296343.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.
1
61
121
181
241
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361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_055502.1 CDS 186..3104 Translation
Target ORF information:
RefSeq Version NM_014687.4 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_014687.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu27916 Clone ID Related Accession (Same CDS sequence) NM_014687.4 , NM_014687.3 Accession Version NM_014687.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1506960000000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 2 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY003277.1, BC033615.1, D86979.3, AC024560.26 and AI796534.1. This sequence is a reference standard in the RefSeqGene project. On Nov 5, 2016 this sequence version replaced NM_014687.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_055502.1 CDS 208..3126 Translation
Target ORF information:
RefSeq Version NM_014687.3 Organism Homo sapiens(human) Definition Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_014687.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38258 Clone ID Related Accession (Same CDS sequence) XM_006713828.3 Accession Version XM_006713828.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X2 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713828.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGG TAGCGAAGGA
TCTAACCTGA CTCACATATC AAAGAACGGA CTGTCAGTGT CACTGGCCTC GATGTTCTCA
GATGCTGACA TCAGAAGGAA CACAGCCTCA AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC
TTCTCCCACT GCTTCCTGCA CTCCACGTCT GCTGAGGCGG TGGCCATGGG GCTCCTGAAG
CAGTTTGAGG GGATGCAGCT TCCAGCCGCC TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT
GATGCCCCTC AGAAGCTCCT GCCCATTCCT GACTCACTGC CCATCTCACC GGATGACGGG
CAGCACGCTG ACATCTACAA GCTGCGGATT CGTGTTCGTG GCAACTTGGA GTGGGCCCCG
CCCCGGCCTC AGATAATTTT TAATGTTCAT CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC
AAGCAGAATT ACCGCTGTGC AGGATGTGGC ATCCGGACTG ACCCTGATTA CATCAAGCGA
CTGCGGTACT GTGAGTACCT GGGCAAGTAC TTCTGCCAGT GCTGCCACGA GAATGCCCAG
ATGGCCATCC CCAGCCGGGT TCTGCGCAAG TGGGACTTCA GCAAGTACTA CGTCAGCAAC
TTCTCCAAGG ACCTGCTCAT TAAGATCTGG AATGATCCTC TCTTCAACGT GCAGGACATA
AACAGTGCCC TCTATAGGAA GGTCAAGCTG CTCAATCAAG TCCGGCTGCT GCGGGTCCAG
CTGTGTCACA TGAAGAACAT GTTCAAGACT TGCCGACTGG CCAAGGAGCT TCTGGATTCC
TTTGACACAG TCCCAGGCCA CCTGACAGAG GACCTCCACC TGTACTCACT GAATGACCTG
ACTGCGACCA GGAAGGGGGA GCTGGGGCCC CGGCTTGCTG AGCTCACCAG GGCAGGGGCT
ACCCATGTGG AGAGATGCAT GCTCTGCCAA GCCAAAGGCT TCATCTGTGA GTTCTGTCAG
AATGAGGATG ACATCATCTT TCCCTTTGAG CTCCATAAGT GCCGGACCTG TGAAGAGTGT
AAAGCGTGTT ACCATAAAGC CTGCTTCAAG TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG
CAGGCCCGGC GGGAGGCACT GGCCAGGCAG AGCCTGGAGT CTTACCTGTC AGACTACGAG
GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA GCCGCCGTCC TGGAGGCCAC CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006713891.1 CDS 203..3196 Translation
Target ORF information:
RefSeq Version XM_006713828.3 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006713828.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGG TAGCGAAGGA
TCTAACCTGA CTCACATATC AAAGAACGGA CTGTCAGTGT CACTGGCCTC GATGTTCTCA
GATGCTGACA TCAGAAGGAA CACAGCCTCA AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC
TTCTCCCACT GCTTCCTGCA CTCCACGTCT GCTGAGGCGG TGGCCATGGG GCTCCTGAAG
CAGTTTGAGG GGATGCAGCT TCCAGCCGCC TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT
GATGCCCCTC AGAAGCTCCT GCCCATTCCT GACTCACTGC CCATCTCACC GGATGACGGG
CAGCACGCTG ACATCTACAA GCTGCGGATT CGTGTTCGTG GCAACTTGGA GTGGGCCCCG
CCCCGGCCTC AGATAATTTT TAATGTTCAT CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC
AAGCAGAATT ACCGCTGTGC AGGATGTGGC ATCCGGACTG ACCCTGATTA CATCAAGCGA
CTGCGGTACT GTGAGTACCT GGGCAAGTAC TTCTGCCAGT GCTGCCACGA GAATGCCCAG
ATGGCCATCC CCAGCCGGGT TCTGCGCAAG TGGGACTTCA GCAAGTACTA CGTCAGCAAC
TTCTCCAAGG ACCTGCTCAT TAAGATCTGG AATGATCCTC TCTTCAACGT GCAGGACATA
AACAGTGCCC TCTATAGGAA GGTCAAGCTG CTCAATCAAG TCCGGCTGCT GCGGGTCCAG
CTGTGTCACA TGAAGAACAT GTTCAAGACT TGCCGACTGG CCAAGGAGCT TCTGGATTCC
TTTGACACAG TCCCAGGCCA CCTGACAGAG GACCTCCACC TGTACTCACT GAATGACCTG
ACTGCGACCA GGAAGGGGGA GCTGGGGCCC CGGCTTGCTG AGCTCACCAG GGCAGGGGCT
ACCCATGTGG AGAGATGCAT GCTCTGCCAA GCCAAAGGCT TCATCTGTGA GTTCTGTCAG
AATGAGGATG ACATCATCTT TCCCTTTGAG CTCCATAAGT GCCGGACCTG TGAAGAGTGT
AAAGCGTGTT ACCATAAAGC CTGCTTCAAG TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG
CAGGCCCGGC GGGAGGCACT GGCCAGGCAG AGCCTGGAGT CTTACCTGTC AGACTACGAG
GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA GCCGCCGTCC TGGAGGCCAC CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38259 Clone ID Related Accession (Same CDS sequence) XM_005269374.3 Accession Version XM_005269374.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X3 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA
GCCGCCGTCC TGGAGGCCAC CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_005269431.1 CDS 203..3166 Translation
Target ORF information:
RefSeq Version XM_005269374.3 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_005269374.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA
GCCGCCGTCC TGGAGGCCAC CTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38260 Clone ID Related Accession (Same CDS sequence) XM_006713829.3 , XM_006713829.4 Accession Version XM_006713829.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X4 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006713892.1 CDS 699..3557 Translation
Target ORF information:
RefSeq Version XM_006713829.3 Organism Homo sapiens(human) Definition Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006713829.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38260 Clone ID Related Accession (Same CDS sequence) XM_006713829.3 , XM_006713829.4 Accession Version XM_006713829.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X4 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006713829.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006713892.1 CDS 547..3405 Translation
Target ORF information:
RefSeq Version XM_006713829.4 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006713829.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38261 Clone ID Related Accession (Same CDS sequence) XM_006713831.3 , XM_006713831.4 , XM_006713830.3 Accession Version XM_006713831.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC
GTCCTGGAGG CCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006713894.1 CDS 866..3403 Translation
Target ORF information:
RefSeq Version XM_006713831.3 Organism Homo sapiens(human) Definition Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X6, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006713831.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC
GTCCTGGAGG CCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38261 Clone ID Related Accession (Same CDS sequence) XM_006713831.3 , XM_006713831.4 , XM_006713830.3 Accession Version XM_006713831.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006713831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC
GTCCTGGAGG CCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006713894.1 CDS 742..3279 Translation
Target ORF information:
RefSeq Version XM_006713831.4 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X6, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006713831.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC
GTCCTGGAGG CCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38261 Clone ID Related Accession (Same CDS sequence) XM_006713831.3 , XM_006713831.4 , XM_006713830.3 Accession Version XM_006713830.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC
GTCCTGGAGG CCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006713893.1 CDS 553..3090 Translation
Target ORF information:
RefSeq Version XM_006713830.3 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X5, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006713830.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC
GTCCTGGAGG CCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu94800 Clone ID Related Accession (Same CDS sequence) XM_017007543.1 , XM_024453838.1 Accession Version XM_017007543.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X6 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016863032.1 CDS 549..3041 Translation
Target ORF information:
RefSeq Version XM_017007543.1 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X7, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017007543.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu94800 Clone ID Related Accession (Same CDS sequence) XM_017007543.1 , XM_024453838.1 Accession Version XM_024453838.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X6 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_024309606.1 CDS 742..3234 Translation
Target ORF information:
RefSeq Version XM_024453838.1 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X8, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_024453838.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu94801 Clone ID Related Accession (Same CDS sequence) XM_017007545.1 , XM_017007545.2 , XM_017007544.1 Accession Version XM_017007545.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC
TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016863034.1 CDS 866..3328 Translation
Target ORF information:
RefSeq Version XM_017007545.1 Organism Homo sapiens(human) Definition Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X9, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017007545.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC
TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu94801 Clone ID Related Accession (Same CDS sequence) XM_017007545.1 , XM_017007545.2 , XM_017007544.1 Accession Version XM_017007545.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017007545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC
TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016863034.1 CDS 742..3204 Translation
Target ORF information:
RefSeq Version XM_017007545.2 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X10, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017007545.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC
TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu94801 Clone ID Related Accession (Same CDS sequence) XM_017007545.1 , XM_017007545.2 , XM_017007544.1 Accession Version XM_017007544.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC
TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016863033.1 CDS 550..3012 Translation
Target ORF information:
RefSeq Version XM_017007544.1 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X9, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017007544.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC
TGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu100758 Clone ID Related Accession (Same CDS sequence) NM_001346873.1 Accession Version NM_001346873.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1564934400000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 3 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC024560.26. This sequence is a reference standard in the RefSeqGene project. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.1208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGGTGGTCGG TACCTGTGCT CAGGGGAAGG CATGTTCCGA
AGACCATCAG AAGGACAGTC CCTCATCAGC TACCTCTCTG AGCAAGACTT CGGCAGCTGT
GCCGACCTGG AAAAGGAGAA TGCCCACTTC AGCATCTCAG AGTCCTTAAT TGCTGCCATC
GAGCTAATGA AGTGCAACAT GATGAGCCAG TGCCTAGAGG AGGAGGAAGT GGAAGAGGAA
GACAGTGATA GAGAGATCCA GGAGCTGAAG CAGAAGATCC GCCTTCGGCG CCAGCAAATC
CGCACCAAGA ACCTGCTCCC CATGTACCAG GAGGCTGAGC ACGGAAGCTT TCGGGTCACC
TCCAGCAGCT CCCAGTTCAG CTCACGTGAT TCGGCACAGC TCTCTGACTC TGGCTCTGCT
GATGAGGTTG ATGAATTTGA AATCCAAGAT GGTAGCGAAG GATCTAACCT GACTCACATA
TCAAAGAACG GACTGTCAGT GTCACTGGCC TCGATGTTCT CAGATGCTGA CATCAGAAGG
AACACAGCCT CAAGCAGCAA ATCCTTCGTT TCCTCCCAGT CCTTCTCCCA CTGCTTCCTG
CACTCCACGT CTGCTGAGGC GGTGGCCATG GGGCTCCTGA AGCAGTTTGA GGGGATGCAG
CTTCCAGCCG CCTCGGAGCT GGAGTGGCTT GTCCCGGAGC ATGATGCCCC TCAGAAGCTC
CTGCCCATTC CTGACTCACT GCCCATCTCA CCGGATGACG GGCAGCACGC TGACATCTAC
AAGCTGCGGA TTCGTGTTCG TGGCAACTTG GAGTGGGCCC CGCCCCGGCC TCAGATAATT
TTTAATGTTC ATCCAGCCCC AACGAGGAAA ATTGCCGTGG CCAAGCAGAA TTACCGCTGT
GCAGGATGTG GCATCCGGAC TGACCCTGAT TACATCAAGC GACTGCGGTA CTGTGAGTAC
CTGGGCAAGT ACTTCTGCCA GTGCTGCCAC GAGAATGCCC AGATGGCCAT CCCCAGCCGG
GTTCTGCGCA AGTGGGACTT CAGCAAGTAC TACGTCAGCA ACTTCTCCAA GGACCTGCTC
ATTAAGATCT GGAATGATCC TCTCTTCAAC GTGCAGGACA TAAACAGTGC CCTCTATAGG
AAGGTCAAGC TGCTCAATCA AGTCCGGCTG CTGCGGGTCC AGCTGTGTCA CATGAAGAAC
ATGTTCAAGA CTTGCCGACT GGCCAAGGAG CTTCTGGATT CCTTTGACAC AGTCCCAGGC
CACCTGACAG AGGACCTCCA CCTGTACTCA CTGAATGACC TGACTGCGAC CAGGAAGGGG
GAGCTGGGGC CCCGGCTTGC TGAGCTCACC AGGGCAGGGG CTACCCATGT GGAGAGATGC
ATGCTCTGCC AAGCCAAAGG CTTCATCTGT GAGTTCTGTC AGAATGAGGA TGACATCATC
TTTCCCTTTG AGCTCCATAA GTGCCGGACC TGTGAAGAGT GTAAAGCGTG TTACCATAAA
GCCTGCTTCA AGTCTGGAAG CTGTCCGCGC TGCGAGCGGC TGCAGGCCCG GCGGGAGGCA
CTGGCCAGGC AGAGCCTGGA GTCTTACCTG TCAGACTACG AGGAGGAGCC CGCGGAAGCG
CTGGCCCTGG AAGCCGCCGT CCTGGAGGCC ACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001333802.1 CDS 208..3243 Translation
Target ORF information:
RefSeq Version NM_001346873.1 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001346873.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGGTGGTCGG TACCTGTGCT CAGGGGAAGG CATGTTCCGA
AGACCATCAG AAGGACAGTC CCTCATCAGC TACCTCTCTG AGCAAGACTT CGGCAGCTGT
GCCGACCTGG AAAAGGAGAA TGCCCACTTC AGCATCTCAG AGTCCTTAAT TGCTGCCATC
GAGCTAATGA AGTGCAACAT GATGAGCCAG TGCCTAGAGG AGGAGGAAGT GGAAGAGGAA
GACAGTGATA GAGAGATCCA GGAGCTGAAG CAGAAGATCC GCCTTCGGCG CCAGCAAATC
CGCACCAAGA ACCTGCTCCC CATGTACCAG GAGGCTGAGC ACGGAAGCTT TCGGGTCACC
TCCAGCAGCT CCCAGTTCAG CTCACGTGAT TCGGCACAGC TCTCTGACTC TGGCTCTGCT
GATGAGGTTG ATGAATTTGA AATCCAAGAT GGTAGCGAAG GATCTAACCT GACTCACATA
TCAAAGAACG GACTGTCAGT GTCACTGGCC TCGATGTTCT CAGATGCTGA CATCAGAAGG
AACACAGCCT CAAGCAGCAA ATCCTTCGTT TCCTCCCAGT CCTTCTCCCA CTGCTTCCTG
CACTCCACGT CTGCTGAGGC GGTGGCCATG GGGCTCCTGA AGCAGTTTGA GGGGATGCAG
CTTCCAGCCG CCTCGGAGCT GGAGTGGCTT GTCCCGGAGC ATGATGCCCC TCAGAAGCTC
CTGCCCATTC CTGACTCACT GCCCATCTCA CCGGATGACG GGCAGCACGC TGACATCTAC
AAGCTGCGGA TTCGTGTTCG TGGCAACTTG GAGTGGGCCC CGCCCCGGCC TCAGATAATT
TTTAATGTTC ATCCAGCCCC AACGAGGAAA ATTGCCGTGG CCAAGCAGAA TTACCGCTGT
GCAGGATGTG GCATCCGGAC TGACCCTGAT TACATCAAGC GACTGCGGTA CTGTGAGTAC
CTGGGCAAGT ACTTCTGCCA GTGCTGCCAC GAGAATGCCC AGATGGCCAT CCCCAGCCGG
GTTCTGCGCA AGTGGGACTT CAGCAAGTAC TACGTCAGCA ACTTCTCCAA GGACCTGCTC
ATTAAGATCT GGAATGATCC TCTCTTCAAC GTGCAGGACA TAAACAGTGC CCTCTATAGG
AAGGTCAAGC TGCTCAATCA AGTCCGGCTG CTGCGGGTCC AGCTGTGTCA CATGAAGAAC
ATGTTCAAGA CTTGCCGACT GGCCAAGGAG CTTCTGGATT CCTTTGACAC AGTCCCAGGC
CACCTGACAG AGGACCTCCA CCTGTACTCA CTGAATGACC TGACTGCGAC CAGGAAGGGG
GAGCTGGGGC CCCGGCTTGC TGAGCTCACC AGGGCAGGGG CTACCCATGT GGAGAGATGC
ATGCTCTGCC AAGCCAAAGG CTTCATCTGT GAGTTCTGTC AGAATGAGGA TGACATCATC
TTTCCCTTTG AGCTCCATAA GTGCCGGACC TGTGAAGAGT GTAAAGCGTG TTACCATAAA
GCCTGCTTCA AGTCTGGAAG CTGTCCGCGC TGCGAGCGGC TGCAGGCCCG GCGGGAGGCA
CTGGCCAGGC AGAGCCTGGA GTCTTACCTG TCAGACTACG AGGAGGAGCC CGCGGAAGCG
CTGGCCCTGG AAGCCGCCGT CCTGGAGGCC ACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38257 Clone ID Related Accession (Same CDS sequence) XM_006713827.3 Accession Version XM_006713827.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006713890.1 CDS 204..3242 Translation
Target ORF information:
RefSeq Version XM_006713827.3 Organism Homo sapiens(human) Definition Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006713827.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
-
PubMed
Rubicon: LC3-associated phagocytosis and beyond.
The FEBS journal285(8)1379-1388(2018 04)
Wong SW,Sil P,Martinez J
Inducible Rubicon facilitates viral replication by antagonizing interferon production.
Cellular & molecular immunology14(7)607-620(2017 Jul)
Wan Y,Cao W,Han T,Ren S,Feng J,Chen T,Wang J,Broering R,Lu M,Zhu Y
HCV induces the expression of Rubicon and UVRAG to temporally regulate the maturation of autophagosomes and viral replication.
PLoS pathogens11(3)e1004764(2015 Mar)
Wang L,Tian Y,Ou JH
A kinase-independent role for EGF receptor in autophagy initiation.
Cell160(1-2)145-60(2015 Jan)
Tan X,Thapa N,Sun Y,Anderson RA
EGFR-mediated Beclin 1 phosphorylation in autophagy suppression, tumor progression, and tumor chemoresistance.
Cell154(6)1269-84(2013 Sep)
Wei Y,Zou Z,Becker N,Anderson M,Sumpter R,Xiao G,Kinch L,Koduru P,Christudass CS,Veltri RW,Grishin NV,Peyton M,Minna J,Bhagat G,Levine B
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