GNG8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNG8
Entrez Gene ID	94235
Full Name	G protein subunit gamma 8
General protein information	Preferred Names G protein subunit gamma 8 Names guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 gamma-9 guanine nucleotide binding protein (G protein), gamma 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.32

mRNA and Protein(s)

mRNA	Protein	Name
XM_017027505.1	XP_016882994.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
XM_017027504.1	XP_016882993.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
XM_017027504.2	XP_016882993.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
XM_017027503.1	XP_016882992.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
XM_011527524.2	XP_011525826.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X1
NM_033258.1	NP_150283.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GNG8	NP_001193483.1
Mus musculus (house mouse)	Gng8	NP_034450.1
Macaca mulatta (Rhesus monkey)	GNG8	XP_001112438.2
Danio rerio (zebrafish)	gng8	NP_001171214.1
Homo sapiens (human)	GNG8	NP_150283.1
Rattus norvegicus (Norway rat)	Gng8	NP_631924.1
Xenopus tropicalis (tropical clawed frog)	gng8	XP_002932676.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary hierarchy of vertebrate-like heterotrimeric G protein families.
Krishnan A, Mustafa A, Almén MS, Fredriksson R, Williams MJ, Schiöth HB
Molecular phylogenetics and evolution9127-40(2015 Oct)

Differential G protein subunit expression by prostate cancer cells and their interaction with CXCR5.
El-Haibi CP, Sharma P, Singh R, Gupta P, Taub DD, Singh S, Lillard JW Jr
Molecular cancer1264(2013 Jun)

Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Complex formation of NDPK B with Gbeta gamma dimers and phosphorylation of His-266 IN Gbeta.
Cuello F, Schulze RA, Heemeyer F, Meyer HE, Lutz S, Jakobs KH, Niroomand F, Wieland T
The Journal of biological chemistry278(9)7220-6(2003 Feb)

Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes.
Hurowitz EH, Melnyk JM, Chen YJ, Kouros-Mehr H, Simon MI, Shizuya H
DNA research : an international journal for rapid publication of reports on genes and genomes7(2)111-20(2000 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNG8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNG8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05381	NM_033258.1 Latest version!	Homo sapiens G protein subunit gamma 8 (GNG8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu05381	XM_017027504.2 Latest version!	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu05381	XM_017027505.1 Latest version!	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu05381	XM_017027503.1 Latest version!	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu74629	XM_011527524.2 Latest version!	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu05381	XM_017027504.1	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNG8 ( NM_033258.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_150283.1 Homo sapiens G protein subunit gamma 8 (GNG8), mRNA.

Price & Availability↑

CloneID	OHu05381
Clone ID Related Accession (Same CDS sequence)	XM_017027505.1 , XM_017027504.1 , XM_017027504.2 , XM_017027503.1 , NM_033258.1
Accession Version	NM_033258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF188179.1. ##Evidence-Data-START## Transcript exon combination :: AF493875.1, BC095514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150283.1
CDS	1..213
Translation	MSNNMAKIAEARKTVEQLKLEVNIDRMKVSQAAAELLAFCETHAKDDPLVTPVPAAENPFRDKRLFCVLL

Target ORF information:

RefSeq Version	NM_033258.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma 8 (GNG8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033258.1

ORF Insert Sequence:

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNG8 ( XM_017027505.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882994.1 Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu05381
Clone ID Related Accession (Same CDS sequence)	XM_017027505.1 , XM_017027504.1 , XM_017027504.2 , XM_017027503.1 , NM_033258.1
Accession Version	XM_017027505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882994.1
CDS	94..306
Translation	MSNNMAKIAEARKTVEQLKLEVNIDRMKVSQAAAELLAFCETHAKDDPLVTPVPAAENPFRDKRLFCVLL

Target ORF information:

RefSeq Version	XM_017027505.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027505.1

ORF Insert Sequence:

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNG8 ( XM_017027504.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882993.1 Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu05381
Clone ID Related Accession (Same CDS sequence)	XM_017027505.1 , XM_017027504.1 , XM_017027504.2 , XM_017027503.1 , NM_033258.1
Accession Version	XM_017027504.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882993.1
CDS	2795..3007
Translation	MSNNMAKIAEARKTVEQLKLEVNIDRMKVSQAAAELLAFCETHAKDDPLVTPVPAAENPFRDKRLFCVLL

Target ORF information:

RefSeq Version	XM_017027504.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027504.1

ORF Insert Sequence:

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNG8 ( XM_017027504.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882993.1 Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu05381
Clone ID Related Accession (Same CDS sequence)	XM_017027505.1 , XM_017027504.1 , XM_017027504.2 , XM_017027503.1 , NM_033258.1
Accession Version	XM_017027504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882993.1
CDS	2794..3006
Translation	MSNNMAKIAEARKTVEQLKLEVNIDRMKVSQAAAELLAFCETHAKDDPLVTPVPAAENPFRDKRLFCVLL

Target ORF information:

RefSeq Version	XM_017027504.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027504.2

ORF Insert Sequence:

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNG8 ( XM_017027503.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882992.1 Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu05381
Clone ID Related Accession (Same CDS sequence)	XM_017027505.1 , XM_017027504.1 , XM_017027504.2 , XM_017027503.1 , NM_033258.1
Accession Version	XM_017027503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882992.1
CDS	140..352
Translation	MSNNMAKIAEARKTVEQLKLEVNIDRMKVSQAAAELLAFCETHAKDDPLVTPVPAAENPFRDKRLFCVLL

Target ORF information:

RefSeq Version	XM_017027503.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027503.1

ORF Insert Sequence:

ATGTCCAACA ACATGGCCAA GATTGCCGAG GCCCGCAAGA CGGTGGAACA GCTGAAGCTG 
GAGGTGAACA TCGACCGCAT GAAGGTGTCG CAGGCAGCAG CGGAACTCCT GGCTTTCTGC 
GAGACGCATG CCAAAGATGA CCCGCTGGTG ACGCCAGTAC CCGCCGCGGA GAACCCCTTC 
CGCGACAAGC GCCTCTTTTG TGTTCTGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNG8 ( XM_011527524.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525826.1 Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu74629
Clone ID Related Accession (Same CDS sequence)	XM_011527524.2
Accession Version	XM_011527524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCCC CTCTGTGCCC CTCCCGCCGC GATCTTAATT CCCAGTTCTC CCTGGAGCCA 
CTGCTAATTG GCCTGGGGGA GGGGCCCCCC TCCTGGCTCT CGCTCTGCCC CCGGGGCCCC 
CACTCCCGCG CGTCTTTTAA CCCCTTCCCC GCCGCAACCA TGTCCAACAA CATGGCCAAG 
ATTGCCGAGG CCCGCAAGAC GGTGGAACAG CTGAAGCTGG AGGTGAACAT CGACCGCATG 
AAGGTGTCGC AGGCAGCAGC GGAACTCCTG GCTTTCTGCG AGACGCATGC CAAAGATGAC 
CCGCTGGTGA CGCCAGTACC CGCCGCGGAG AACCCCTTCC GCGACAAGCG CCTCTTTTGT 
GTTCTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525826.1
CDS	402..773
Translation	MRPPLCPSRRDLNSQFSLEPLLIGLGEGPPSWLSLCPRGPHSRASFNPFPAATMSNNMAKIAEARKTVEQLKLEVNIDRMKVSQAAAELLAFCETHAKDDPLVTPVPAAENPFRDKRLFCVLL

Target ORF information:

RefSeq Version	XM_011527524.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein subunit gamma 8 (GNG8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527524.2

ORF Insert Sequence:

ATGAGGCCCC CTCTGTGCCC CTCCCGCCGC GATCTTAATT CCCAGTTCTC CCTGGAGCCA 
CTGCTAATTG GCCTGGGGGA GGGGCCCCCC TCCTGGCTCT CGCTCTGCCC CCGGGGCCCC 
CACTCCCGCG CGTCTTTTAA CCCCTTCCCC GCCGCAACCA TGTCCAACAA CATGGCCAAG 
ATTGCCGAGG CCCGCAAGAC GGTGGAACAG CTGAAGCTGG AGGTGAACAT CGACCGCATG 
AAGGTGTCGC AGGCAGCAGC GGAACTCCTG GCTTTCTGCG AGACGCATGC CAAAGATGAC 
CCGCTGGTGA CGCCAGTACC CGCCGCGGAG AACCCCTTCC GCGACAAGCG CCTCTTTTGT 
GTTCTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary hierarchy of vertebrate-like heterotrimeric G protein families.
Molecular phylogenetics and evolution9127-40(2015 Oct)
Krishnan A,Mustafa A,Almén MS,Fredriksson R,Williams MJ,Schiöth HB

Differential G protein subunit expression by prostate cancer cells and their interaction with CXCR5.
Molecular cancer1264(2013 Jun)
El-Haibi CP,Sharma P,Singh R,Gupta P,Taub DD,Singh S,Lillard JW Jr

Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Complex formation of NDPK B with Gbeta gamma dimers and phosphorylation of His-266 IN Gbeta.
The Journal of biological chemistry278(9)7220-6(2003 Feb)
Cuello F,Schulze RA,Heemeyer F,Meyer HE,Lutz S,Jakobs KH,Niroomand F,Wieland T

Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes.
DNA research : an international journal for rapid publication of reports on genes and genomes7(2)111-20(2000 Apr)
Hurowitz EH,Melnyk JM,Chen YJ,Kouros-Mehr H,Simon MI,Shizuya H

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GNG8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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