CD19 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD19
Entrez Gene ID	930
Full Name	CD19 molecule
Synonyms	B4,CVID3
General protein information	Preferred Names CD19 molecule Names B-lymphocyte antigen CD19 B-lymphocyte surface antigen B4 T-cell surface antigen Leu-12 differentiation antigen CD19
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Summary	Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008].
Disorder MIM:	107265
Disorder Html:	Immunodeficiency, common variable, 3, 613493 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011545981.2	XP_011544283.1	B-lymphocyte antigen CD19 isoform X3
XM_011545981.1	XP_011544283.1	B-lymphocyte antigen CD19 isoform X3
XM_017023893.1	XP_016879382.1	B-lymphocyte antigen CD19 isoform X2
NM_001178098.1	NP_001171569.1	B-lymphocyte antigen CD19 isoform 1 precursor
NM_001178098.2	NP_001171569.1	B-lymphocyte antigen CD19 isoform 1 precursor
XM_006721103.3	XP_006721166.1	B-lymphocyte antigen CD19 isoform X1
NM_001770.5	NP_001761.3	B-lymphocyte antigen CD19 isoform 2 precursor
NM_001770.6	NP_001761.3	B-lymphocyte antigen CD19 isoform 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CD19	NP_001761.3
Mus musculus (house mouse)	Cd19	NP_033974.2
Rattus norvegicus (Norway rat)	Cd19	NP_001013255.2
Pan troglodytes (chimpanzee)	CD19	XP_510906.3
Macaca mulatta (Rhesus monkey)	CD19	XP_001103043.1
Canis lupus familiaris (dog)	CD19	XP_860299.1
Bos taurus (cattle)	CD19	NP_001232927.1

Gene Ontology
Bibliography

Related articles in PubMed

Decreased number of CD19
Li T, Yu Z, Qu Z, Zhang N, Crew R, Jiang Y
Molecular immunology112233-239(2019 08)

Development of Inducible CD19-CAR T Cells with a Tet-On System for Controlled Activity and Enhanced Clinical Safety.
Gu X, He D, Li C, Wang H, Yang G
International journal of molecular sciences19(11)(2018 Nov)

CD19 Alterations Emerging after CD19-Directed Immunotherapy Cause Retention of the Misfolded Protein in the Endoplasmic Reticulum.
Bagashev A, Sotillo E, Tang CH, Black KL, Perazzelli J, Seeholzer SH, Argon Y, Barrett DM, Grupp SA, Hu CC, Thomas-Tikhonenko A
Molecular and cellular biology38(21)(2018 11)

Heterogeneity of surface CD19 and CD22 expression in B lymphoblastic leukemia.
Rosenthal J, Naqvi AS, Luo M, Wertheim G, Paessler M, Thomas-Tikhonenko A, Rheingold SR, Pillai V
American journal of hematology93(11)E352-E355(2018 11)

Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells.
He X, Kläsener K, Iype JM, Becker M, Maity PC, Cavallari M, Nielsen PJ, Yang J, Reth M
The EMBO journal37(11)(2018 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CD19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27320	NM_001178098.2 Latest version!	Homo sapiens CD19 molecule (CD19), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu27366	NM_001770.6 Latest version!	Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu49218	XM_006721103.3 Latest version!	Homo sapiens CD19 molecule (CD19), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu56709	XM_011545981.2 Latest version!	Homo sapiens CD19 molecule (CD19), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu86394	XM_017023893.1 Latest version!	Homo sapiens CD19 molecule (CD19), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu56709	XM_011545981.1	Homo sapiens CD19 molecule (CD19), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu27320	NM_001178098.1	Homo sapiens CD19 molecule (CD19), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu27366	NM_001770.5	Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD19 ( NM_001178098.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171569.1 Homo sapiens CD19 molecule (CD19), transcript variant 1, mRNA.

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CloneID	OHu27320
Clone ID Related Accession (Same CDS sequence)	NM_001178098.1 , NM_001178098.2
Accession Version	NM_001178098.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549641600000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA959905.1, BC006338.2, AK313577.1 and AA760729.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.175126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGCAGG GTCCCAGTCC 
TATGAGGATA TGAGAGGAAT CCTGTATGCA GCCCCCCAGC TCCGCTCCAT TCGGGGCCAG 
CCTGGACCCA ATCATGAGGA AGATGCAGAC TCTTATGAGA ACATGGATAA TCCCGATGGG 
CCAGACCCAG CCTGGGGAGG AGGGGGCCGC ATGGGCACCT GGAGCACCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171569.1
CDS	63..1736
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLAGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR

Target ORF information:

RefSeq Version	NM_001178098.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178098.1

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGCAGG GTCCCAGTCC 
TATGAGGATA TGAGAGGAAT CCTGTATGCA GCCCCCCAGC TCCGCTCCAT TCGGGGCCAG 
CCTGGACCCA ATCATGAGGA AGATGCAGAC TCTTATGAGA ACATGGATAA TCCCGATGGG 
CCAGACCCAG CCTGGGGAGG AGGGGGCCGC ATGGGCACCT GGAGCACCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD19 ( NM_001178098.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171569.1 Homo sapiens CD19 molecule (CD19), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27320
Clone ID Related Accession (Same CDS sequence)	NM_001178098.1 , NM_001178098.2
Accession Version	NM_001178098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006338.2 and AK313577.1. On Jun 2, 2019 this sequence version replaced NM_001178098.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.175126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGCAGG GTCCCAGTCC 
TATGAGGATA TGAGAGGAAT CCTGTATGCA GCCCCCCAGC TCCGCTCCAT TCGGGGCCAG 
CCTGGACCCA ATCATGAGGA AGATGCAGAC TCTTATGAGA ACATGGATAA TCCCGATGGG 
CCAGACCCAG CCTGGGGAGG AGGGGGCCGC ATGGGCACCT GGAGCACCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171569.1
CDS	31..1704
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLAGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR

Target ORF information:

RefSeq Version	NM_001178098.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178098.2

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGCAGG GTCCCAGTCC 
TATGAGGATA TGAGAGGAAT CCTGTATGCA GCCCCCCAGC TCCGCTCCAT TCGGGGCCAG 
CCTGGACCCA ATCATGAGGA AGATGCAGAC TCTTATGAGA ACATGGATAA TCCCGATGGG 
CCAGACCCAG CCTGGGGAGG AGGGGGCCGC ATGGGCACCT GGAGCACCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD19 ( NM_001770.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001761.3 Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27366
Clone ID Related Accession (Same CDS sequence)	NM_001770.5 , NM_001770.6
Accession Version	NM_001770.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1561392000000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA959905.1, BC006338.2 and AA760729.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced NM_001770.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006338.2, SRR1163655.355529.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGGGTC CCAGTCCTAT 
GAGGATATGA GAGGAATCCT GTATGCAGCC CCCCAGCTCC GCTCCATTCG GGGCCAGCCT 
GGACCCAATC ATGAGGAAGA TGCAGACTCT TATGAGAACA TGGATAATCC CGATGGGCCA 
GACCCAGCCT GGGGAGGAGG GGGCCGCATG GGCACCTGGA GCACCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001761.3
CDS	63..1733
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR

Target ORF information:

RefSeq Version	NM_001770.5
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001770.5

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGGGTC CCAGTCCTAT 
GAGGATATGA GAGGAATCCT GTATGCAGCC CCCCAGCTCC GCTCCATTCG GGGCCAGCCT 
GGACCCAATC ATGAGGAAGA TGCAGACTCT TATGAGAACA TGGATAATCC CGATGGGCCA 
GACCCAGCCT GGGGAGGAGG GGGCCGCATG GGCACCTGGA GCACCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD19 ( NM_001770.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001761.3 Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.

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CloneID	OHu27366
Clone ID Related Accession (Same CDS sequence)	NM_001770.5 , NM_001770.6
Accession Version	NM_001770.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006338.2. On Aug 31, 2019 this sequence version replaced NM_001770.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006338.2, M21097.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGGGTC CCAGTCCTAT 
GAGGATATGA GAGGAATCCT GTATGCAGCC CCCCAGCTCC GCTCCATTCG GGGCCAGCCT 
GGACCCAATC ATGAGGAAGA TGCAGACTCT TATGAGAACA TGGATAATCC CGATGGGCCA 
GACCCAGCCT GGGGAGGAGG GGGCCGCATG GGCACCTGGA GCACCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001761.3
CDS	31..1701
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR

Target ORF information:

RefSeq Version	NM_001770.6
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001770.6

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGTACTA 
TGGCACTGGC TGCTGAGGAC TGGTGGCTGG AAGGTCTCAG CTGTGACTTT GGCTTATCTG 
ATCTTCTGCC TGTGTTCCCT TGTGGGCATT CTTCATCTTC AAAGAGCCCT GGTCCTGAGG 
AGGAAAAGAA AGCGAATGAC TGACCCCACC AGGAGATTCT TCAAAGTGAC GCCTCCCCCA 
GGAAGCGGGC CCCAGAACCA GTACGGGAAC GTGCTGTCTC TCCCCACACC CACCTCAGGC 
CTCGGACGCG CCCAGCGTTG GGCCGCAGGC CTGGGGGGCA CTGCCCCGTC TTATGGAAAC 
CCGAGCAGCG ACGTCCAGGC GGATGGAGCC TTGGGGTCCC GGAGCCCGCC GGGAGTGGGC 
CCAGAAGAAG AGGAAGGGGA GGGCTATGAG GAACCTGACA GTGAGGAGGA CTCCGAGTTC 
TATGAGAACG ACTCCAACCT TGGGCAGGAC CAGCTCTCCC AGGATGGCAG CGGCTACGAG 
AACCCTGAGG ATGAGCCCCT GGGTCCTGAG GATGAAGACT CCTTCTCCAA CGCTGAGTCT 
TATGAGAACG AGGATGAAGA GCTGACCCAG CCGGTCGCCA GGACAATGGA CTTCCTGAGC 
CCTCATGGGT CAGCCTGGGA CCCCAGCCGG GAAGCAACCT CCCTGGGGTC CCAGTCCTAT 
GAGGATATGA GAGGAATCCT GTATGCAGCC CCCCAGCTCC GCTCCATTCG GGGCCAGCCT 
GGACCCAATC ATGAGGAAGA TGCAGACTCT TATGAGAACA TGGATAATCC CGATGGGCCA 
GACCCAGCCT GGGGAGGAGG GGGCCGCATG GGCACCTGGA GCACCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD19 ( XM_006721103.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721166.1 Homo sapiens CD19 molecule (CD19), transcript variant X1, mRNA.

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CloneID	OHu49218
Clone ID Related Accession (Same CDS sequence)	XM_006721103.3
Accession Version	XM_006721103.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721103.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGGG GAGCTGTTCC GGTGGAATGT TTCGGACCTA 
GGTGGCCTGG GCTGTGGCCT GAAGAACAGG TCCTCAGAGG GCCCCAGCTC CCCTTCCGGG 
AAGCTCATGA GCCCCAAGCT GTATGTGTGG GCCAAAGACC GCCCTGAGAT CTGGGAGGGA 
GAGCCTCCGT GTCTCCCACC GAGGGACAGC CTGAACCAGA GCCTCAGCCA GGACCTCACC 
ATGGCCCCTG GCTCCACACT CTGGCTGTCC TGTGGGGTAC CCCCTGACTC TGTGTCCAGG 
GGCCCCCTCT CCTGGACCCA TGTGCACCCC AAGGGGCCTA AGTCATTGCT GAGCCTAGAG 
CTGAAGGACG ATCGCCCGGC CAGAGATATG TGGGTAATGG AGACGGGTCT GTTGTTGCCC 
CGGGCCACAG CTCAAGACGC TGGAAAGTAT TATTGTCACC GTGGCAACCT GACCATGTCA 
TTCCACCTGG AGATCACTGC TCGGCCAGTA CTATGGCACT GGCTGCTGAG GACTGGTGGC 
TGGAAGGTCT CAGCTGTGAC TTTGGCTTAT CTGATCTTCT GCCTGTGTTC CCTTGTGGGC 
ATTCTTCATC TTCAAAGAGC CCTGGTCCTG AGGAGGAAAA GAAAGCGAAT GACTGACCCC 
ACCAGGAGAT TCTTCAAAGT GACGCCTCCC CCAGGAAGCG GGCCCCAGAA CCAGTACGGG 
AACGTGCTGT CTCTCCCCAC ACCCACCTCA GGCCTCGGAC GCGCCCAGCG TTGGGCCGCA 
GGCCTGGGGG GCACTGCCCC GTCTTATGGA AACCCGAGCA GCGACGTCCA GGCGGATGGA 
GCCTTGGGGT CCCGGAGCCC GCCGGGAGTG GGCCCAGAAG AAGAGGAAGG GGAGGGCTAT 
GAGGAACCTG ACAGTGAGGA GGACTCCGAG TTCTATGAGA ACGACTCCAA CCTTGGGCAG 
GACCAGCTCT CCCAGGATGG CAGCGGCTAC GAGAACCCTG AGGATGAGCC CCTGGGTCCT 
GAGGATGAAG ACTCCTTCTC CAACGCTGAG TCTTATGAGA ACGAGGATGA AGAGCTGACC 
CAGCCGGTCG CCAGGACAAT GGACTTCCTG AGCCCTCATG GGTCAGCCTG GGACCCCAGC 
CGGGAAGCAA CCTCCCTGGC AGGGTCCCAG TCCTATGAGG ATATGAGAGG AATCCTGTAT 
GCAGCCCCCC AGCTCCGCTC CATTCGGGGC CAGCCTGGAC CCAATCATGA GGAAGATGCA 
GACTCTTATG AGAACATGGA TAATCCCGAT GGGCCAGACC CAGCCTGGGG AGGAGGGGGC 
CGCATGGGCA CCTGGAGCAC CAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721166.1
CDS	267..1673
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLAGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR

Target ORF information:

RefSeq Version	XM_006721103.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721103.3

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGGG GAGCTGTTCC GGTGGAATGT TTCGGACCTA 
GGTGGCCTGG GCTGTGGCCT GAAGAACAGG TCCTCAGAGG GCCCCAGCTC CCCTTCCGGG 
AAGCTCATGA GCCCCAAGCT GTATGTGTGG GCCAAAGACC GCCCTGAGAT CTGGGAGGGA 
GAGCCTCCGT GTCTCCCACC GAGGGACAGC CTGAACCAGA GCCTCAGCCA GGACCTCACC 
ATGGCCCCTG GCTCCACACT CTGGCTGTCC TGTGGGGTAC CCCCTGACTC TGTGTCCAGG 
GGCCCCCTCT CCTGGACCCA TGTGCACCCC AAGGGGCCTA AGTCATTGCT GAGCCTAGAG 
CTGAAGGACG ATCGCCCGGC CAGAGATATG TGGGTAATGG AGACGGGTCT GTTGTTGCCC 
CGGGCCACAG CTCAAGACGC TGGAAAGTAT TATTGTCACC GTGGCAACCT GACCATGTCA 
TTCCACCTGG AGATCACTGC TCGGCCAGTA CTATGGCACT GGCTGCTGAG GACTGGTGGC 
TGGAAGGTCT CAGCTGTGAC TTTGGCTTAT CTGATCTTCT GCCTGTGTTC CCTTGTGGGC 
ATTCTTCATC TTCAAAGAGC CCTGGTCCTG AGGAGGAAAA GAAAGCGAAT GACTGACCCC 
ACCAGGAGAT TCTTCAAAGT GACGCCTCCC CCAGGAAGCG GGCCCCAGAA CCAGTACGGG 
AACGTGCTGT CTCTCCCCAC ACCCACCTCA GGCCTCGGAC GCGCCCAGCG TTGGGCCGCA 
GGCCTGGGGG GCACTGCCCC GTCTTATGGA AACCCGAGCA GCGACGTCCA GGCGGATGGA 
GCCTTGGGGT CCCGGAGCCC GCCGGGAGTG GGCCCAGAAG AAGAGGAAGG GGAGGGCTAT 
GAGGAACCTG ACAGTGAGGA GGACTCCGAG TTCTATGAGA ACGACTCCAA CCTTGGGCAG 
GACCAGCTCT CCCAGGATGG CAGCGGCTAC GAGAACCCTG AGGATGAGCC CCTGGGTCCT 
GAGGATGAAG ACTCCTTCTC CAACGCTGAG TCTTATGAGA ACGAGGATGA AGAGCTGACC 
CAGCCGGTCG CCAGGACAAT GGACTTCCTG AGCCCTCATG GGTCAGCCTG GGACCCCAGC 
CGGGAAGCAA CCTCCCTGGC AGGGTCCCAG TCCTATGAGG ATATGAGAGG AATCCTGTAT 
GCAGCCCCCC AGCTCCGCTC CATTCGGGGC CAGCCTGGAC CCAATCATGA GGAAGATGCA 
GACTCTTATG AGAACATGGA TAATCCCGAT GGGCCAGACC CAGCCTGGGG AGGAGGGGGC 
CGCATGGGCA CCTGGAGCAC CAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD19 ( XM_011545981.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544283.1 Homo sapiens CD19 molecule (CD19), transcript variant X4, mRNA.

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CloneID	OHu56709
Clone ID Related Accession (Same CDS sequence)	XM_011545981.2 , XM_011545981.1
Accession Version	XM_011545981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGCCCCT 
TCCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544283.1
CDS	46..894
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPAPSP

Target ORF information:

RefSeq Version	XM_011545981.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545981.2

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGCCCCT 
TCCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD19 ( XM_011545981.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544283.1 Homo sapiens CD19 molecule (CD19), transcript variant X4, mRNA.

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CloneID	OHu56709
Clone ID Related Accession (Same CDS sequence)	XM_011545981.2 , XM_011545981.1
Accession Version	XM_011545981.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGCCCCT 
TCCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544283.1
CDS	63..911
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPAPSP

Target ORF information:

RefSeq Version	XM_011545981.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545981.1

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGAG GGAGATAACG CTGTGCTGCA GTGCCTCAAG 
GGGACCTCAG ATGGCCCCAC TCAGCAGCTG ACCTGGTCTC GGGAGTCCCC GCTTAAACCC 
TTCTTAAAAC TCAGCCTGGG GCTGCCAGGC CTGGGAATCC ACATGAGGCC CCTGGCCATC 
TGGCTTTTCA TCTTCAACGT CTCTCAACAG ATGGGGGGCT TCTACCTGTG CCAGCCGGGG 
CCCCCCTCTG AGAAGGCCTG GCAGCCTGGC TGGACAGTCA ATGTGGAGGG CAGCGGGGAG 
CTGTTCCGGT GGAATGTTTC GGACCTAGGT GGCCTGGGCT GTGGCCTGAA GAACAGGTCC 
TCAGAGGGCC CCAGCTCCCC TTCCGGGAAG CTCATGAGCC CCAAGCTGTA TGTGTGGGCC 
AAAGACCGCC CTGAGATCTG GGAGGGAGAG CCTCCGTGTC TCCCACCGAG GGACAGCCTG 
AACCAGAGCC TCAGCCAGGA CCTCACCATG GCCCCTGGCT CCACACTCTG GCTGTCCTGT 
GGGGTACCCC CTGACTCTGT GTCCAGGGGC CCCCTCTCCT GGACCCATGT GCACCCCAAG 
GGGCCTAAGT CATTGCTGAG CCTAGAGCTG AAGGACGATC GCCCGGCCAG AGATATGTGG 
GTAATGGAGA CGGGTCTGTT GTTGCCCCGG GCCACAGCTC AAGACGCTGG AAAGTATTAT 
TGTCACCGTG GCAACCTGAC CATGTCATTC CACCTGGAGA TCACTGCTCG GCCAGCCCCT 
TCCCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD19 ( XM_017023893.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879382.1 Homo sapiens CD19 molecule (CD19), transcript variant X2, mRNA.

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CloneID	OHu86394
Clone ID Related Accession (Same CDS sequence)	XM_017023893.1
Accession Version	XM_017023893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	B-lymphocyte antigen CD19 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGGG GAGCTGTTCC GGTGGAATGT TTCGGACCTA 
GGTGGCCTGG GCTGTGGCCT GAAGAACAGG TCCTCAGAGG GCCCCAGCTC CCCTTCCGGG 
AAGCTCATGA GCCCCAAGCT GTATGTGTGG GCCAAAGACC GCCCTGAGAT CTGGGAGGGA 
GAGCCTCCGT GTCTCCCACC GAGGGACAGC CTGAACCAGA GCCTCAGCCA GGACCTCACC 
ATGGCCCCTG GCTCCACACT CTGGCTGTCC TGTGGGGTAC CCCCTGACTC TGTGTCCAGG 
GGCCCCCTCT CCTGGACCCA TGTGCACCCC AAGGGGCCTA AGTCATTGCT GAGCCTAGAG 
CTGAAGGACG ATCGCCCGGC CAGAGATATG TGGGTAATGG AGACGGGTCT GTTGTTGCCC 
CGGGCCACAG CTCAAGACGC TGGAAAGTAT TATTGTCACC GTGGCAACCT GACCATGTCA 
TTCCACCTGG AGATCACTGC TCGGCCAGTA CTATGGCACT GGCTGCTGAG GACTGGTGGC 
TGGAAGGTCT CAGCTGTGAC TTTGGCTTAT CTGATCTTCT GCCTGTGTTC CCTTGTGGGC 
ATTCTTCATC TTCAAAGAGC CCTGGTCCTG AGGAGGAAAA GAAAGCGAAT GACTGACCCC 
ACCAGGAGAT TCTTCAAAGT GACGCCTCCC CCAGGAAGCG GGCCCCAGAA CCAGTACGGG 
AACGTGCTGT CTCTCCCCAC ACCCACCTCA GGCCTCGGAC GCGCCCAGCG TTGGGCCGCA 
GGCCTGGGGG GCACTGCCCC GTCTTATGGA AACCCGAGCA GCGACGTCCA GGCGGATGGA 
GCCTTGGGGT CCCGGAGCCC GCCGGGAGTG GGCCCAGAAG AAGAGGAAGG GGAGGGCTAT 
GAGGAACCTG ACAGTGAGGA GGACTCCGAG TTCTATGAGA ACGACTCCAA CCTTGGGCAG 
GACCAGCTCT CCCAGGATGG CAGCGGCTAC GAGAACCCTG AGGATGAGCC CCTGGGTCCT 
GAGGATGAAG ACTCCTTCTC CAACGCTGAG TCTTATGAGA ACGAGGATGA AGAGCTGACC 
CAGCCGGTCG CCAGGACAAT GGACTTCCTG AGCCCTCATG GGTCAGCCTG GGACCCCAGC 
CGGGAAGCAA CCTCCCTGGG GTCCCAGTCC TATGAGGATA TGAGAGGAAT CCTGTATGCA 
GCCCCCCAGC TCCGCTCCAT TCGGGGCCAG CCTGGACCCA ATCATGAGGA AGATGCAGAC 
TCTTATGAGA ACATGGATAA TCCCGATGGG CCAGACCCAG CCTGGGGAGG AGGGGGCCGC 
ATGGGCACCT GGAGCACCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879382.1
CDS	131..1534
Translation	MPPPRLLFFLLFLTPMEVRPEEPLVVKVEGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR

Target ORF information:

RefSeq Version	XM_017023893.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CD19 molecule (CD19), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023893.1

ORF Insert Sequence:

ATGCCACCTC CTCGCCTCCT CTTCTTCCTC CTCTTCCTCA CCCCCATGGA AGTCAGGCCC 
GAGGAACCTC TAGTGGTGAA GGTGGAAGGG GAGCTGTTCC GGTGGAATGT TTCGGACCTA 
GGTGGCCTGG GCTGTGGCCT GAAGAACAGG TCCTCAGAGG GCCCCAGCTC CCCTTCCGGG 
AAGCTCATGA GCCCCAAGCT GTATGTGTGG GCCAAAGACC GCCCTGAGAT CTGGGAGGGA 
GAGCCTCCGT GTCTCCCACC GAGGGACAGC CTGAACCAGA GCCTCAGCCA GGACCTCACC 
ATGGCCCCTG GCTCCACACT CTGGCTGTCC TGTGGGGTAC CCCCTGACTC TGTGTCCAGG 
GGCCCCCTCT CCTGGACCCA TGTGCACCCC AAGGGGCCTA AGTCATTGCT GAGCCTAGAG 
CTGAAGGACG ATCGCCCGGC CAGAGATATG TGGGTAATGG AGACGGGTCT GTTGTTGCCC 
CGGGCCACAG CTCAAGACGC TGGAAAGTAT TATTGTCACC GTGGCAACCT GACCATGTCA 
TTCCACCTGG AGATCACTGC TCGGCCAGTA CTATGGCACT GGCTGCTGAG GACTGGTGGC 
TGGAAGGTCT CAGCTGTGAC TTTGGCTTAT CTGATCTTCT GCCTGTGTTC CCTTGTGGGC 
ATTCTTCATC TTCAAAGAGC CCTGGTCCTG AGGAGGAAAA GAAAGCGAAT GACTGACCCC 
ACCAGGAGAT TCTTCAAAGT GACGCCTCCC CCAGGAAGCG GGCCCCAGAA CCAGTACGGG 
AACGTGCTGT CTCTCCCCAC ACCCACCTCA GGCCTCGGAC GCGCCCAGCG TTGGGCCGCA 
GGCCTGGGGG GCACTGCCCC GTCTTATGGA AACCCGAGCA GCGACGTCCA GGCGGATGGA 
GCCTTGGGGT CCCGGAGCCC GCCGGGAGTG GGCCCAGAAG AAGAGGAAGG GGAGGGCTAT 
GAGGAACCTG ACAGTGAGGA GGACTCCGAG TTCTATGAGA ACGACTCCAA CCTTGGGCAG 
GACCAGCTCT CCCAGGATGG CAGCGGCTAC GAGAACCCTG AGGATGAGCC CCTGGGTCCT 
GAGGATGAAG ACTCCTTCTC CAACGCTGAG TCTTATGAGA ACGAGGATGA AGAGCTGACC 
CAGCCGGTCG CCAGGACAAT GGACTTCCTG AGCCCTCATG GGTCAGCCTG GGACCCCAGC 
CGGGAAGCAA CCTCCCTGGG GTCCCAGTCC TATGAGGATA TGAGAGGAAT CCTGTATGCA 
GCCCCCCAGC TCCGCTCCAT TCGGGGCCAG CCTGGACCCA ATCATGAGGA AGATGCAGAC 
TCTTATGAGA ACATGGATAA TCCCGATGGG CCAGACCCAG CCTGGGGAGG AGGGGGCCGC 
ATGGGCACCT GGAGCACCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decreased number of CD19
Molecular immunology112233-239(2019 08)
Li T,Yu Z,Qu Z,Zhang N,Crew R,Jiang Y

Development of Inducible CD19-CAR T Cells with a Tet-On System for Controlled Activity and Enhanced Clinical Safety.
International journal of molecular sciences19(11)(2018 Nov)
Gu X,He D,Li C,Wang H,Yang G

CD19 Alterations Emerging after CD19-Directed Immunotherapy Cause Retention of the Misfolded Protein in the Endoplasmic Reticulum.
Molecular and cellular biology38(21)(2018 11)
Bagashev A,Sotillo E,Tang CH,Black KL,Perazzelli J,Seeholzer SH,Argon Y,Barrett DM,Grupp SA,Hu CC,Thomas-Tikhonenko A

Heterogeneity of surface CD19 and CD22 expression in B lymphoblastic leukemia.
American journal of hematology93(11)E352-E355(2018 11)
Rosenthal J,Naqvi AS,Luo M,Wertheim G,Paessler M,Thomas-Tikhonenko A,Rheingold SR,Pillai V

Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells.
The EMBO journal37(11)(2018 06)
He X,Kläsener K,Iype JM,Becker M,Maity PC,Cavallari M,Nielsen PJ,Yang J,Reth M

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CD19 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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