| Gene Symbol | CH25H |
| Entrez Gene ID | 9023 |
| Full Name | cholesterol 25-hydroxylase |
| Synonyms | C25H |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: |
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CH25H cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following CH25H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CH25H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu29872 
Clone ID Related Accession (Same CDS sequence) NM_003956.3 , NM_003956.4 Accession Version NM_003956.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 819bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1550246400000 Organism Homo sapiens(human) Product cholesterol 25-hydroxylase Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017843.1. On May 13, 2006 this sequence version replaced NM_003956.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC017843.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_003947.1 CDS 23..841 Translation 
Target ORF information:
RefSeq Version NM_003956.3 Organism Homo sapiens(human) Definition Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_003956.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu29872 Clone ID Related Accession (Same CDS sequence) NM_003956.3 , NM_003956.4 Accession Version NM_003956.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 819bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1577808000000 Organism Homo sapiens(human) Product cholesterol 25-hydroxylase Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513533.15 and BC017843.1. On May 17, 2019 this sequence version replaced NM_003956.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1660809.218929.1, DRR138524.275451.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371852.4/ ENSP00000360918.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_003947.1 CDS 26..844 Translation
Target ORF information:
RefSeq Version NM_003956.4 Organism Homo sapiens(human) Definition Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_003956.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAGCTGCC ACAACTGCTC CGACCCCCAG GTCCTTTGCA GCTCCGGGCA GCTGTTCCTG
CAGCCCCTCT GGGACCACCT GAGGAGCTGG GAGGCCCTCC TACAGTCGCC CTTCTTCCCG
GTCATCTTCT CCATCACCAC ATACGTGGGC TTTTGCCTGC CCTTCGTGGT CCTGGATATC
CTGTGCTCCT GGGTGCCCGC CCTGCGGCGC TACAAGATCC ACCCTGACTT CTCGCCATCC
GCGCAGCAGC TGCTACCTTG CCTGGGGCAG ACCCTCTACC AGCATGTGAT GTTTGTGTTC
CCCGTGACGC TGCTGCATTG GGCCCGCAGC CCGGCCCTCC TGCCCCACGA AGCTCCCGAG
CTGCTCCTGC TGCTGCACCA CATCCTGTTC TGCCTGCTAC TCTTCGACAT GGAGTTCTTC
GTGTGGCACC TGCTGCACCA CAAGGTGCCC TGGCTGTACC GCACCTTCCA CAAGGTGCAC
CACCAGAACT CGTCCTCGTT CGCGCTGGCA ACGCAGTATA TGAGCGTCTG GGAACTGTTT
TCTTTGGGCT TCTTCGACAT GATGAACGTC ACACTGCTCG GGTGCCACCC GCTCACCACC
CTGACCTTCC ACGTGGTCAA CATCTGGCTT TCCGTGGAGG ACCACTCCGG CTACAACTTC
CCTTGGTCCA CTCACAGACT GGTGCCCTTC GGGTGGTACG GGGGTGTGGT GCACCACGAC
CTGCATCACT CTCACTTTAA CTGCAACTTC GCTCCGTACT TTACACACTG GGACAAAATA
CTGGGAACGC TGCGGACTGC ATCTGTCCCA GCGCGGTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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PubMed
Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry.
Archives of virology164(12)2963-2974(2019 Dec)
Yuan Y,Wang Z,Tian B,Zhou M,Fu ZF,Zhao L
Association between selected cholesterol-related gene polymorphisms and Alzheimer's disease in a Turkish cohort.
Molecular biology reports46(2)1701-1707(2019 Apr)
Guven G,Vurgun E,Bilgic B,Hanagasi H,Gurvit H,Ozer E,Lohmann E,Erginel-Unaltuna N
An Interferon-Driven Oxysterol-Based Defense against Tumor-Derived Extracellular Vesicles.
Cancer cell35(1)33-45.e6(2019 01)
Ortiz A,Gui J,Zahedi F,Yu P,Cho C,Bhattacharya S,Carbone CJ,Yu Q,Katlinski KV,Katlinskaya YV,Handa S,Haas V,Volk SW,Brice AK,Wals K,Matheson NJ,Antrobus R,Ludwig S,Whiteside TL,Sander C,Tarhini AA,Kirkwood JM,Lehner PJ,Guo W,Rui H,Minn AJ,Koumenis C,Diehl JA,Fuchs SY
25-Hydroxycholesterol Production by the Cholesterol-25-Hydroxylase Interferon-Stimulated Gene Restricts Mammalian Reovirus Infection.
Journal of virology92(18)(2018 09)
Doms A,Sanabria T,Hansen JN,Altan-Bonnet N,Holm GH
Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function.
Redox biology11111-117(2017 04)
Zerbinati C,Caponecchia L,Puca R,Ciacciarelli M,Salacone P,Sebastianelli A,Pastore A,Palleschi G,Petrozza V,Porta N,Rago R,Carbone A,Iuliano L
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