MAP4K3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP4K3
Entrez Gene ID	8491
Full Name	mitogen-activated protein kinase kinase kinase kinase 3
Synonyms	GLK,MAPKKKK3,MEKKK 3,MEKKK3,RAB8IPL1
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase kinase 3 Names mitogen-activated protein kinase kinase kinase kinase 3 MAPK/ERK kinase kinase kinase 3 MEK kinase kinase 3 germinal center kinase-like kinase germinal center kinase-related protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p22.1
Summary	This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	604921

mRNA and Protein(s)

mRNA	Protein	Name
NM_003618.4	NP_003609.2	mitogen-activated protein kinase kinase kinase kinase 3 isoform 1
XM_024453183.1	XP_024308951.1	mitogen-activated protein kinase kinase kinase kinase 3 isoform X1
NM_001270425.1	NP_001257354.1	mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
NM_001270425.2	NP_001257354.1	mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
NM_003618.3	NP_003609.2	mitogen-activated protein kinase kinase kinase kinase 3 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Map4k3	NP_596898.1
Macaca mulatta (Rhesus monkey)	LOC713952	XP_001103468.2
Gallus gallus (chicken)	MAP4K3	XP_003640980.2
Bos taurus (cattle)	MAP4K3	NP_001178091.1
Pan troglodytes (chimpanzee)	MAP4K3	XP_515427.4
Canis lupus familiaris (dog)	MAP4K3	XP_005630430.1
Mus musculus (house mouse)	Map4k3	NP_001074826.1
Drosophila melanogaster (fruit fly)	hppy	NP_611399.2
Caenorhabditis elegans (roundworm)	gck-2	NP_504721.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010837	XP_309868.4
Xenopus tropicalis (tropical clawed frog)	map4k3	NP_001123819.1
Homo sapiens (human)	MAP4K3	NP_003609.2
Danio rerio (zebrafish)	map4k3b	XP_001921635.2

Gene Ontology
Bibliography

Related articles in PubMed

Over-expression of miR-206 decreases the Euthyrox-resistance by targeting MAP4K3 in papillary thyroid carcinoma.
Liu F, Yin R, Chen X, Chen W, Qian Y, Zhao Y, Jiang Y, Ma D, Hu T, Yu T, Zhu Y, Zhang Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie114108605(2019 Jun)

MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB.
Hsu CL, Lee EX, Gordon KL, Paz EA, Shen WC, Ohnishi K, Meisenhelder J, Hunter T, La Spada AR
Nature communications9(1)942(2018 03)

Germinal-center kinase-like kinase co-crystal structure reveals a swapped activation loop and C-terminal extension.
Marcotte D, Rushe M, M Arduini R, Lukacs C, Atkins K, Sun X, Little K, Cullivan M, Paramasivam M, Patterson TA, Hesson T, D McKee T, May-Dracka TL, Xin Z, Bertolotti-Ciarlet A, Bhisetti GR, Lyssikatos JP, Silvian LF
Protein science : a publication of the Protein Society26(2)152-162(2017 02)

MiR-199a-5p and let-7c cooperatively inhibit migration and invasion by targeting MAP4K3 in hepatocellular carcinoma.
Liu L, Lu L, Zheng A, Xie J, Xue Q, Wang F, Wang X, Zhou H, Tong X, Li Y, Zhu X, Wu G
Oncotarget8(8)13666-13677(2017 Feb)

Prediction of early hepatocellular carcinoma recurrence using germinal center kinase-like kinase.
Ho CH, Chuang HC, Wu IC, Tsai HW, Lin YJ, Sun HY, Young KC, Chiu YC, Cheng PN, Liu WC, Tan TH, Chang TT
Oncotarget7(31)49765-49776(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAP4K3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu104987	XM_024453183.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu11613	NM_001270425.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu20653	NM_003618.4 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
View Old Accession Versions >>
OHu11613	NM_001270425.1	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu20653	NM_003618.3	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP4K3 ( XM_024453183.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308951.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu104987
Clone ID Related Accession (Same CDS sequence)	XM_024453183.1
Accession Version	XM_024453183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCTA CTGAGGATGA AAATCAAGGA ACAATCAAGA GATGTCCCAT GTCAGGGAGC 
CCAGCAAAGC CATCCCAAGT TCCACCTAGA CCACCACCTC CCAGATTACC CCCACACAAA 
CCTGTTGCCT TAGGAAATGG AATGAGCTCC TTCCAGTTAA ATGGTGAACG AGATGGCTCA 
TTATGTCAAC AACAGAATGA ACATAGAGGC ACAAACCTTT CAAGAAAAGA AAAGAAAGAT 
GTACCAAAGC CTATTAGTAA TGGTCTTCCT CCAACACCTA AAGTGCATAT GGGTGCATGT 
TTTTCAAAAG TTTTTAATGG GTGTCCCTTG AAAATTCACT GTGCATCATC ATGGATAAAC 
CCAGATACAA GAGATCAGTA CTTGATATTT GGTGCCGAAG AAGGGATTTA TACCCTCAAT 
CTTAATGAAC TTCATGAAAC ATCAATGGAA CAGCTATTCC CTCGAAGGTG TACATGGTTG 
TATGTAATGA ACAATTGCTT GCTATCAATA TCTGGTAAAG CTTCTCAGCT TTATTCCCAT 
AATTTACCAG GGCTTTTTGA TTATGCAAGA CAAATGCAAA AGTTACCTGT TGCTATTCCA 
GCACACAAAC TCCCTGACAG AATACTGCCA AGGAAATTTT CTGTATCAGC AAAAATCCCT 
GAAACCAAAT GGTGCCAGAA GTGTTGTGTT GTAAGAAATC CTTACACGGG CCATAAATAC 
CTATGTGGAG CACTTCAGAC TAGCATTGTT CTATTAGAAT GGGTTGAACC AATGCAGAAA 
TTTATGTTAA TTAAGCACAT AGATTTTCCT ATACCATGTC CACTTAGAAT GTTTGAAATG 
CTGGTAGTTC CTGAACAGGA GTACCCTTTA GTTTGTGTTG GTGTCAGTAG AGGTAGAGAC 
TTCAACCAAG TGGTTCGATT TGAGACGGTC AATCCAAATT CTACCTCTTC ATGGTTTACA 
GAATCAGATA CCCCACAGAC AAATGTTACT CATGTAACCC AACTGGAGAG AGATACCATC 
CTTGTATGCT TGGACTGTTG TATAAAAATA GTAAATCTCC AAGGAAGATT AAAATCTAGC 
AGGAAATTGT CATCAGAACT CACCTTTGAT TTCCAGATTG AATCAATAGT GTGCCTACAA 
GACAGTGTGC TAGCTTTCTG GAAACATGGA ATGCAAGGTA GAAGTTTTAG ATCTAATGAG 
GTAACACAAG AAATTTCAGA TAGCACAAGA ATTTTCAGGC TGCTTGGATC TGACAGGGTC 
GTGGTTTTGG AAAGTAGGCC AACTGATAAC CCCACAGCAA ATAGCAATTT GTACATCCTG 
GCGGGTCATG AAAACAGTTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308951.1
CDS	696..2039
Translation	MHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

Target ORF information:

RefSeq Version	XM_024453183.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453183.1

ORF Insert Sequence:

ATGCATTCTA CTGAGGATGA AAATCAAGGA ACAATCAAGA GATGTCCCAT GTCAGGGAGC 
CCAGCAAAGC CATCCCAAGT TCCACCTAGA CCACCACCTC CCAGATTACC CCCACACAAA 
CCTGTTGCCT TAGGAAATGG AATGAGCTCC TTCCAGTTAA ATGGTGAACG AGATGGCTCA 
TTATGTCAAC AACAGAATGA ACATAGAGGC ACAAACCTTT CAAGAAAAGA AAAGAAAGAT 
GTACCAAAGC CTATTAGTAA TGGTCTTCCT CCAACACCTA AAGTGCATAT GGGTGCATGT 
TTTTCAAAAG TTTTTAATGG GTGTCCCTTG AAAATTCACT GTGCATCATC ATGGATAAAC 
CCAGATACAA GAGATCAGTA CTTGATATTT GGTGCCGAAG AAGGGATTTA TACCCTCAAT 
CTTAATGAAC TTCATGAAAC ATCAATGGAA CAGCTATTCC CTCGAAGGTG TACATGGTTG 
TATGTAATGA ACAATTGCTT GCTATCAATA TCTGGTAAAG CTTCTCAGCT TTATTCCCAT 
AATTTACCAG GGCTTTTTGA TTATGCAAGA CAAATGCAAA AGTTACCTGT TGCTATTCCA 
GCACACAAAC TCCCTGACAG AATACTGCCA AGGAAATTTT CTGTATCAGC AAAAATCCCT 
GAAACCAAAT GGTGCCAGAA GTGTTGTGTT GTAAGAAATC CTTACACGGG CCATAAATAC 
CTATGTGGAG CACTTCAGAC TAGCATTGTT CTATTAGAAT GGGTTGAACC AATGCAGAAA 
TTTATGTTAA TTAAGCACAT AGATTTTCCT ATACCATGTC CACTTAGAAT GTTTGAAATG 
CTGGTAGTTC CTGAACAGGA GTACCCTTTA GTTTGTGTTG GTGTCAGTAG AGGTAGAGAC 
TTCAACCAAG TGGTTCGATT TGAGACGGTC AATCCAAATT CTACCTCTTC ATGGTTTACA 
GAATCAGATA CCCCACAGAC AAATGTTACT CATGTAACCC AACTGGAGAG AGATACCATC 
CTTGTATGCT TGGACTGTTG TATAAAAATA GTAAATCTCC AAGGAAGATT AAAATCTAGC 
AGGAAATTGT CATCAGAACT CACCTTTGAT TTCCAGATTG AATCAATAGT GTGCCTACAA 
GACAGTGTGC TAGCTTTCTG GAAACATGGA ATGCAAGGTA GAAGTTTTAG ATCTAATGAG 
GTAACACAAG AAATTTCAGA TAGCACAAGA ATTTTCAGGC TGCTTGGATC TGACAGGGTC 
GTGGTTTTGG AAAGTAGGCC AACTGATAAC CCCACAGCAA ATAGCAATTT GTACATCCTG 
GCGGGTCATG AAAACAGTTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K3 ( NM_001270425.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257354.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11613
Clone ID Related Accession (Same CDS sequence)	NM_001270425.1 , NM_001270425.2
Accession Version	NM_001270425.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA409370.1, AK314487.1, AC007684.3, AK021553.1 and AC079615.6. Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071579.1, SRR1660803.121725.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTGATC TGCAACTGGA ATATGGACAA 
GGACACCAAG GTGGTTACTT TTTAGGTGCA AACAAGAGTC TTCTCAAGTC TGTTGAAGAA 
GAATTGCATC AGCGAGGACA CGTCGCACAT TTAGAAGATG ATGAAGGAGA TGATGATGAA 
TCTAAACACT CAACTCTGAA AGCAAAAATT CCACCTCCTT TGCCACCAAA GCCTAAGTCT 
ATCTTCATAC CACAGGAAAT GCATTCTACT GAGGATGAAA ATCAAGGAAC AATCAAGAGA 
TGTCCCATGT CAGGGAGCCC AGCAAAGCCA TCCCAAGTTC CACCTAGACC ACCACCTCCC 
AGATTACCCC CACACAAACC TGTTGCCTTA GGAAATGGAA TGAGCTCCTT CCAGTTAAAT 
GGTGAACGAG ATGGCTCATT ATGTCAACAA CAGAATGAAC ATAGAGGCAC AAACCTTTCA 
AGAAAAGAAA AGAAAGATGT ACCAAAGCCT ATTAGTAATG GTCTTCCTCC AACACCTAAA 
GTGCATATGG GTGCATGTTT TTCAAAAGTT TTTAATGGGT GTCCCTTGAA AATTCACTGT 
GCATCATCAT GGATAAACCC AGATACAAGA GATCAGTACT TGATATTTGG TGCCGAAGAA 
GGGATTTATA CCCTCAATCT TAATGAACTT CATGAAACAT CAATGGAACA GCTATTCCCT 
CGAAGGTGTA CATGGTTGTA TGTAATGAAC AATTGCTTGC TATCAATATC TGGTAAAGCT 
TCTCAGCTTT ATTCCCATAA TTTACCAGGG CTTTTTGATT ATGCAAGACA AATGCAAAAG 
TTACCTGTTG CTATTCCAGC ACACAAACTC CCTGACAGAA TACTGCCAAG GAAATTTTCT 
GTATCAGCAA AAATCCCTGA AACCAAATGG TGCCAGAAGT GTTGTGTTGT AAGAAATCCT 
TACACGGGCC ATAAATACCT ATGTGGAGCA CTTCAGACTA GCATTGTTCT ATTAGAATGG 
GTTGAACCAA TGCAGAAATT TATGTTAATT AAGCACATAG ATTTTCCTAT ACCATGTCCA 
CTTAGAATGT TTGAAATGCT GGTAGTTCCT GAACAGGAGT ACCCTTTAGT TTGTGTTGGT 
GTCAGTAGAG GTAGAGACTT CAACCAAGTG GTTCGATTTG AGACGGTCAA TCCAAATTCT 
ACCTCTTCAT GGTTTACAGA ATCAGATACC CCACAGACAA ATGTTACTCA TGTAACCCAA 
CTGGAGAGAG ATACCATCCT TGTATGCTTG GACTGTTGTA TAAAAATAGT AAATCTCCAA 
GGAAGATTAA AATCTAGCAG GAAATTGTCA TCAGAACTCA CCTTTGATTT CCAGATTGAA 
TCAATAGTGT GCCTACAAGA CAGTGTGCTA GCTTTCTGGA AACATGGAAT GCAAGGTAGA 
AGTTTTAGAT CTAATGAGGT AACACAAGAA ATTTCAGATA GCACAAGAAT TTTCAGGCTG 
CTTGGATCTG ACAGGGTCGT GGTTTTGGAA AGTAGGCCAA CTGATAACCC CACAGCAAAT 
AGCAATTTGT ACATCCTGGC GGGTCATGAA AACAGTTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257354.1
CDS	326..2947
Translation	MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

Target ORF information:

RefSeq Version	NM_001270425.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270425.1

ORF Insert Sequence:

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTGATC TGCAACTGGA ATATGGACAA 
GGACACCAAG GTGGTTACTT TTTAGGTGCA AACAAGAGTC TTCTCAAGTC TGTTGAAGAA 
GAATTGCATC AGCGAGGACA CGTCGCACAT TTAGAAGATG ATGAAGGAGA TGATGATGAA 
TCTAAACACT CAACTCTGAA AGCAAAAATT CCACCTCCTT TGCCACCAAA GCCTAAGTCT 
ATCTTCATAC CACAGGAAAT GCATTCTACT GAGGATGAAA ATCAAGGAAC AATCAAGAGA 
TGTCCCATGT CAGGGAGCCC AGCAAAGCCA TCCCAAGTTC CACCTAGACC ACCACCTCCC 
AGATTACCCC CACACAAACC TGTTGCCTTA GGAAATGGAA TGAGCTCCTT CCAGTTAAAT 
GGTGAACGAG ATGGCTCATT ATGTCAACAA CAGAATGAAC ATAGAGGCAC AAACCTTTCA 
AGAAAAGAAA AGAAAGATGT ACCAAAGCCT ATTAGTAATG GTCTTCCTCC AACACCTAAA 
GTGCATATGG GTGCATGTTT TTCAAAAGTT TTTAATGGGT GTCCCTTGAA AATTCACTGT 
GCATCATCAT GGATAAACCC AGATACAAGA GATCAGTACT TGATATTTGG TGCCGAAGAA 
GGGATTTATA CCCTCAATCT TAATGAACTT CATGAAACAT CAATGGAACA GCTATTCCCT 
CGAAGGTGTA CATGGTTGTA TGTAATGAAC AATTGCTTGC TATCAATATC TGGTAAAGCT 
TCTCAGCTTT ATTCCCATAA TTTACCAGGG CTTTTTGATT ATGCAAGACA AATGCAAAAG 
TTACCTGTTG CTATTCCAGC ACACAAACTC CCTGACAGAA TACTGCCAAG GAAATTTTCT 
GTATCAGCAA AAATCCCTGA AACCAAATGG TGCCAGAAGT GTTGTGTTGT AAGAAATCCT 
TACACGGGCC ATAAATACCT ATGTGGAGCA CTTCAGACTA GCATTGTTCT ATTAGAATGG 
GTTGAACCAA TGCAGAAATT TATGTTAATT AAGCACATAG ATTTTCCTAT ACCATGTCCA 
CTTAGAATGT TTGAAATGCT GGTAGTTCCT GAACAGGAGT ACCCTTTAGT TTGTGTTGGT 
GTCAGTAGAG GTAGAGACTT CAACCAAGTG GTTCGATTTG AGACGGTCAA TCCAAATTCT 
ACCTCTTCAT GGTTTACAGA ATCAGATACC CCACAGACAA ATGTTACTCA TGTAACCCAA 
CTGGAGAGAG ATACCATCCT TGTATGCTTG GACTGTTGTA TAAAAATAGT AAATCTCCAA 
GGAAGATTAA AATCTAGCAG GAAATTGTCA TCAGAACTCA CCTTTGATTT CCAGATTGAA 
TCAATAGTGT GCCTACAAGA CAGTGTGCTA GCTTTCTGGA AACATGGAAT GCAAGGTAGA 
AGTTTTAGAT CTAATGAGGT AACACAAGAA ATTTCAGATA GCACAAGAAT TTTCAGGCTG 
CTTGGATCTG ACAGGGTCGT GGTTTTGGAA AGTAGGCCAA CTGATAACCC CACAGCAAAT 
AGCAATTTGT ACATCCTGGC GGGTCATGAA AACAGTTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K3 ( NM_001270425.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257354.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11613
Clone ID Related Accession (Same CDS sequence)	NM_001270425.1 , NM_001270425.2
Accession Version	NM_001270425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA409370.1, AK314487.1, AC007684.3, AK021553.1 and AC079615.6. On May 31, 2019 this sequence version replaced NM_001270425.1. Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071579.1, SRR1660805.237018.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTGATC TGCAACTGGA ATATGGACAA 
GGACACCAAG GTGGTTACTT TTTAGGTGCA AACAAGAGTC TTCTCAAGTC TGTTGAAGAA 
GAATTGCATC AGCGAGGACA CGTCGCACAT TTAGAAGATG ATGAAGGAGA TGATGATGAA 
TCTAAACACT CAACTCTGAA AGCAAAAATT CCACCTCCTT TGCCACCAAA GCCTAAGTCT 
ATCTTCATAC CACAGGAAAT GCATTCTACT GAGGATGAAA ATCAAGGAAC AATCAAGAGA 
TGTCCCATGT CAGGGAGCCC AGCAAAGCCA TCCCAAGTTC CACCTAGACC ACCACCTCCC 
AGATTACCCC CACACAAACC TGTTGCCTTA GGAAATGGAA TGAGCTCCTT CCAGTTAAAT 
GGTGAACGAG ATGGCTCATT ATGTCAACAA CAGAATGAAC ATAGAGGCAC AAACCTTTCA 
AGAAAAGAAA AGAAAGATGT ACCAAAGCCT ATTAGTAATG GTCTTCCTCC AACACCTAAA 
GTGCATATGG GTGCATGTTT TTCAAAAGTT TTTAATGGGT GTCCCTTGAA AATTCACTGT 
GCATCATCAT GGATAAACCC AGATACAAGA GATCAGTACT TGATATTTGG TGCCGAAGAA 
GGGATTTATA CCCTCAATCT TAATGAACTT CATGAAACAT CAATGGAACA GCTATTCCCT 
CGAAGGTGTA CATGGTTGTA TGTAATGAAC AATTGCTTGC TATCAATATC TGGTAAAGCT 
TCTCAGCTTT ATTCCCATAA TTTACCAGGG CTTTTTGATT ATGCAAGACA AATGCAAAAG 
TTACCTGTTG CTATTCCAGC ACACAAACTC CCTGACAGAA TACTGCCAAG GAAATTTTCT 
GTATCAGCAA AAATCCCTGA AACCAAATGG TGCCAGAAGT GTTGTGTTGT AAGAAATCCT 
TACACGGGCC ATAAATACCT ATGTGGAGCA CTTCAGACTA GCATTGTTCT ATTAGAATGG 
GTTGAACCAA TGCAGAAATT TATGTTAATT AAGCACATAG ATTTTCCTAT ACCATGTCCA 
CTTAGAATGT TTGAAATGCT GGTAGTTCCT GAACAGGAGT ACCCTTTAGT TTGTGTTGGT 
GTCAGTAGAG GTAGAGACTT CAACCAAGTG GTTCGATTTG AGACGGTCAA TCCAAATTCT 
ACCTCTTCAT GGTTTACAGA ATCAGATACC CCACAGACAA ATGTTACTCA TGTAACCCAA 
CTGGAGAGAG ATACCATCCT TGTATGCTTG GACTGTTGTA TAAAAATAGT AAATCTCCAA 
GGAAGATTAA AATCTAGCAG GAAATTGTCA TCAGAACTCA CCTTTGATTT CCAGATTGAA 
TCAATAGTGT GCCTACAAGA CAGTGTGCTA GCTTTCTGGA AACATGGAAT GCAAGGTAGA 
AGTTTTAGAT CTAATGAGGT AACACAAGAA ATTTCAGATA GCACAAGAAT TTTCAGGCTG 
CTTGGATCTG ACAGGGTCGT GGTTTTGGAA AGTAGGCCAA CTGATAACCC CACAGCAAAT 
AGCAATTTGT ACATCCTGGC GGGTCATGAA AACAGTTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257354.1
CDS	299..2920
Translation	MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

Target ORF information:

RefSeq Version	NM_001270425.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270425.2

ORF Insert Sequence:

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTGATC TGCAACTGGA ATATGGACAA 
GGACACCAAG GTGGTTACTT TTTAGGTGCA AACAAGAGTC TTCTCAAGTC TGTTGAAGAA 
GAATTGCATC AGCGAGGACA CGTCGCACAT TTAGAAGATG ATGAAGGAGA TGATGATGAA 
TCTAAACACT CAACTCTGAA AGCAAAAATT CCACCTCCTT TGCCACCAAA GCCTAAGTCT 
ATCTTCATAC CACAGGAAAT GCATTCTACT GAGGATGAAA ATCAAGGAAC AATCAAGAGA 
TGTCCCATGT CAGGGAGCCC AGCAAAGCCA TCCCAAGTTC CACCTAGACC ACCACCTCCC 
AGATTACCCC CACACAAACC TGTTGCCTTA GGAAATGGAA TGAGCTCCTT CCAGTTAAAT 
GGTGAACGAG ATGGCTCATT ATGTCAACAA CAGAATGAAC ATAGAGGCAC AAACCTTTCA 
AGAAAAGAAA AGAAAGATGT ACCAAAGCCT ATTAGTAATG GTCTTCCTCC AACACCTAAA 
GTGCATATGG GTGCATGTTT TTCAAAAGTT TTTAATGGGT GTCCCTTGAA AATTCACTGT 
GCATCATCAT GGATAAACCC AGATACAAGA GATCAGTACT TGATATTTGG TGCCGAAGAA 
GGGATTTATA CCCTCAATCT TAATGAACTT CATGAAACAT CAATGGAACA GCTATTCCCT 
CGAAGGTGTA CATGGTTGTA TGTAATGAAC AATTGCTTGC TATCAATATC TGGTAAAGCT 
TCTCAGCTTT ATTCCCATAA TTTACCAGGG CTTTTTGATT ATGCAAGACA AATGCAAAAG 
TTACCTGTTG CTATTCCAGC ACACAAACTC CCTGACAGAA TACTGCCAAG GAAATTTTCT 
GTATCAGCAA AAATCCCTGA AACCAAATGG TGCCAGAAGT GTTGTGTTGT AAGAAATCCT 
TACACGGGCC ATAAATACCT ATGTGGAGCA CTTCAGACTA GCATTGTTCT ATTAGAATGG 
GTTGAACCAA TGCAGAAATT TATGTTAATT AAGCACATAG ATTTTCCTAT ACCATGTCCA 
CTTAGAATGT TTGAAATGCT GGTAGTTCCT GAACAGGAGT ACCCTTTAGT TTGTGTTGGT 
GTCAGTAGAG GTAGAGACTT CAACCAAGTG GTTCGATTTG AGACGGTCAA TCCAAATTCT 
ACCTCTTCAT GGTTTACAGA ATCAGATACC CCACAGACAA ATGTTACTCA TGTAACCCAA 
CTGGAGAGAG ATACCATCCT TGTATGCTTG GACTGTTGTA TAAAAATAGT AAATCTCCAA 
GGAAGATTAA AATCTAGCAG GAAATTGTCA TCAGAACTCA CCTTTGATTT CCAGATTGAA 
TCAATAGTGT GCCTACAAGA CAGTGTGCTA GCTTTCTGGA AACATGGAAT GCAAGGTAGA 
AGTTTTAGAT CTAATGAGGT AACACAAGAA ATTTCAGATA GCACAAGAAT TTTCAGGCTG 
CTTGGATCTG ACAGGGTCGT GGTTTTGGAA AGTAGGCCAA CTGATAACCC CACAGCAAAT 
AGCAATTTGT ACATCCTGGC GGGTCATGAA AACAGTTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K3 ( NM_003618.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003609.2 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20653
Clone ID Related Accession (Same CDS sequence)	NM_003618.4 , NM_003618.3
Accession Version	NM_003618.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA409370.1, AK291467.1, AC007684.3, AK021553.1 and AC079615.6. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003618.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060880.1, SRR1660809.155177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263881.8/ ENSP00000263881.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTCCCG ACAGTGATGG TTTTTTGGAC 
AGTTCAGAAG AAATATACTA CACTGCAAGA TCTAATCTGG ATCTGCAACT GGAATATGGA 
CAAGGACACC AAGGTGGTTA CTTTTTAGGT GCAAACAAGA GTCTTCTCAA GTCTGTTGAA 
GAAGAATTGC ATCAGCGAGG ACACGTCGCA CATTTAGAAG ATGATGAAGG AGATGATGAT 
GAATCTAAAC ACTCAACTCT GAAAGCAAAA ATTCCACCTC CTTTGCCACC AAAGCCTAAG 
TCTATCTTCA TACCACAGGA AATGCATTCT ACTGAGGATG AAAATCAAGG AACAATCAAG 
AGATGTCCCA TGTCAGGGAG CCCAGCAAAG CCATCCCAAG TTCCACCTAG ACCACCACCT 
CCCAGATTAC CCCCACACAA ACCTGTTGCC TTAGGAAATG GAATGAGCTC CTTCCAGTTA 
AATGGTGAAC GAGATGGCTC ATTATGTCAA CAACAGAATG AACATAGAGG CACAAACCTT 
TCAAGAAAAG AAAAGAAAGA TGTACCAAAG CCTATTAGTA ATGGTCTTCC TCCAACACCT 
AAAGTGCATA TGGGTGCATG TTTTTCAAAA GTTTTTAATG GGTGTCCCTT GAAAATTCAC 
TGTGCATCAT CATGGATAAA CCCAGATACA AGAGATCAGT ACTTGATATT TGGTGCCGAA 
GAAGGGATTT ATACCCTCAA TCTTAATGAA CTTCATGAAA CATCAATGGA ACAGCTATTC 
CCTCGAAGGT GTACATGGTT GTATGTAATG AACAATTGCT TGCTATCAAT ATCTGGTAAA 
GCTTCTCAGC TTTATTCCCA TAATTTACCA GGGCTTTTTG ATTATGCAAG ACAAATGCAA 
AAGTTACCTG TTGCTATTCC AGCACACAAA CTCCCTGACA GAATACTGCC AAGGAAATTT 
TCTGTATCAG CAAAAATCCC TGAAACCAAA TGGTGCCAGA AGTGTTGTGT TGTAAGAAAT 
CCTTACACGG GCCATAAATA CCTATGTGGA GCACTTCAGA CTAGCATTGT TCTATTAGAA 
TGGGTTGAAC CAATGCAGAA ATTTATGTTA ATTAAGCACA TAGATTTTCC TATACCATGT 
CCACTTAGAA TGTTTGAAAT GCTGGTAGTT CCTGAACAGG AGTACCCTTT AGTTTGTGTT 
GGTGTCAGTA GAGGTAGAGA CTTCAACCAA GTGGTTCGAT TTGAGACGGT CAATCCAAAT 
TCTACCTCTT CATGGTTTAC AGAATCAGAT ACCCCACAGA CAAATGTTAC TCATGTAACC 
CAACTGGAGA GAGATACCAT CCTTGTATGC TTGGACTGTT GTATAAAAAT AGTAAATCTC 
CAAGGAAGAT TAAAATCTAG CAGGAAATTG TCATCAGAAC TCACCTTTGA TTTCCAGATT 
GAATCAATAG TGTGCCTACA AGACAGTGTG CTAGCTTTCT GGAAACATGG AATGCAAGGT 
AGAAGTTTTA GATCTAATGA GGTAACACAA GAAATTTCAG ATAGCACAAG AATTTTCAGG 
CTGCTTGGAT CTGACAGGGT CGTGGTTTTG GAAAGTAGGC CAACTGATAA CCCCACAGCA 
AATAGCAATT TGTACATCCT GGCGGGTCAT GAAAACAGTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003609.2
CDS	299..2983
Translation	MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

Target ORF information:

RefSeq Version	NM_003618.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003618.4

ORF Insert Sequence:

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTCCCG ACAGTGATGG TTTTTTGGAC 
AGTTCAGAAG AAATATACTA CACTGCAAGA TCTAATCTGG ATCTGCAACT GGAATATGGA 
CAAGGACACC AAGGTGGTTA CTTTTTAGGT GCAAACAAGA GTCTTCTCAA GTCTGTTGAA 
GAAGAATTGC ATCAGCGAGG ACACGTCGCA CATTTAGAAG ATGATGAAGG AGATGATGAT 
GAATCTAAAC ACTCAACTCT GAAAGCAAAA ATTCCACCTC CTTTGCCACC AAAGCCTAAG 
TCTATCTTCA TACCACAGGA AATGCATTCT ACTGAGGATG AAAATCAAGG AACAATCAAG 
AGATGTCCCA TGTCAGGGAG CCCAGCAAAG CCATCCCAAG TTCCACCTAG ACCACCACCT 
CCCAGATTAC CCCCACACAA ACCTGTTGCC TTAGGAAATG GAATGAGCTC CTTCCAGTTA 
AATGGTGAAC GAGATGGCTC ATTATGTCAA CAACAGAATG AACATAGAGG CACAAACCTT 
TCAAGAAAAG AAAAGAAAGA TGTACCAAAG CCTATTAGTA ATGGTCTTCC TCCAACACCT 
AAAGTGCATA TGGGTGCATG TTTTTCAAAA GTTTTTAATG GGTGTCCCTT GAAAATTCAC 
TGTGCATCAT CATGGATAAA CCCAGATACA AGAGATCAGT ACTTGATATT TGGTGCCGAA 
GAAGGGATTT ATACCCTCAA TCTTAATGAA CTTCATGAAA CATCAATGGA ACAGCTATTC 
CCTCGAAGGT GTACATGGTT GTATGTAATG AACAATTGCT TGCTATCAAT ATCTGGTAAA 
GCTTCTCAGC TTTATTCCCA TAATTTACCA GGGCTTTTTG ATTATGCAAG ACAAATGCAA 
AAGTTACCTG TTGCTATTCC AGCACACAAA CTCCCTGACA GAATACTGCC AAGGAAATTT 
TCTGTATCAG CAAAAATCCC TGAAACCAAA TGGTGCCAGA AGTGTTGTGT TGTAAGAAAT 
CCTTACACGG GCCATAAATA CCTATGTGGA GCACTTCAGA CTAGCATTGT TCTATTAGAA 
TGGGTTGAAC CAATGCAGAA ATTTATGTTA ATTAAGCACA TAGATTTTCC TATACCATGT 
CCACTTAGAA TGTTTGAAAT GCTGGTAGTT CCTGAACAGG AGTACCCTTT AGTTTGTGTT 
GGTGTCAGTA GAGGTAGAGA CTTCAACCAA GTGGTTCGAT TTGAGACGGT CAATCCAAAT 
TCTACCTCTT CATGGTTTAC AGAATCAGAT ACCCCACAGA CAAATGTTAC TCATGTAACC 
CAACTGGAGA GAGATACCAT CCTTGTATGC TTGGACTGTT GTATAAAAAT AGTAAATCTC 
CAAGGAAGAT TAAAATCTAG CAGGAAATTG TCATCAGAAC TCACCTTTGA TTTCCAGATT 
GAATCAATAG TGTGCCTACA AGACAGTGTG CTAGCTTTCT GGAAACATGG AATGCAAGGT 
AGAAGTTTTA GATCTAATGA GGTAACACAA GAAATTTCAG ATAGCACAAG AATTTTCAGG 
CTGCTTGGAT CTGACAGGGT CGTGGTTTTG GAAAGTAGGC CAACTGATAA CCCCACAGCA 
AATAGCAATT TGTACATCCT GGCGGGTCAT GAAAACAGTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K3 ( NM_003618.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003609.2 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 1, mRNA.

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CloneID	OHu20653
Clone ID Related Accession (Same CDS sequence)	NM_003618.4 , NM_003618.3
Accession Version	NM_003618.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA409370.1, AK291467.1, AC007684.3, AK021553.1 and AC079615.6. This sequence is a reference standard in the RefSeqGene project. On Jul 7, 2012 this sequence version replaced NM_003618.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060880.1, SRR1660809.155177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTCCCG ACAGTGATGG TTTTTTGGAC 
AGTTCAGAAG AAATATACTA CACTGCAAGA TCTAATCTGG ATCTGCAACT GGAATATGGA 
CAAGGACACC AAGGTGGTTA CTTTTTAGGT GCAAACAAGA GTCTTCTCAA GTCTGTTGAA 
GAAGAATTGC ATCAGCGAGG ACACGTCGCA CATTTAGAAG ATGATGAAGG AGATGATGAT 
GAATCTAAAC ACTCAACTCT GAAAGCAAAA ATTCCACCTC CTTTGCCACC AAAGCCTAAG 
TCTATCTTCA TACCACAGGA AATGCATTCT ACTGAGGATG AAAATCAAGG AACAATCAAG 
AGATGTCCCA TGTCAGGGAG CCCAGCAAAG CCATCCCAAG TTCCACCTAG ACCACCACCT 
CCCAGATTAC CCCCACACAA ACCTGTTGCC TTAGGAAATG GAATGAGCTC CTTCCAGTTA 
AATGGTGAAC GAGATGGCTC ATTATGTCAA CAACAGAATG AACATAGAGG CACAAACCTT 
TCAAGAAAAG AAAAGAAAGA TGTACCAAAG CCTATTAGTA ATGGTCTTCC TCCAACACCT 
AAAGTGCATA TGGGTGCATG TTTTTCAAAA GTTTTTAATG GGTGTCCCTT GAAAATTCAC 
TGTGCATCAT CATGGATAAA CCCAGATACA AGAGATCAGT ACTTGATATT TGGTGCCGAA 
GAAGGGATTT ATACCCTCAA TCTTAATGAA CTTCATGAAA CATCAATGGA ACAGCTATTC 
CCTCGAAGGT GTACATGGTT GTATGTAATG AACAATTGCT TGCTATCAAT ATCTGGTAAA 
GCTTCTCAGC TTTATTCCCA TAATTTACCA GGGCTTTTTG ATTATGCAAG ACAAATGCAA 
AAGTTACCTG TTGCTATTCC AGCACACAAA CTCCCTGACA GAATACTGCC AAGGAAATTT 
TCTGTATCAG CAAAAATCCC TGAAACCAAA TGGTGCCAGA AGTGTTGTGT TGTAAGAAAT 
CCTTACACGG GCCATAAATA CCTATGTGGA GCACTTCAGA CTAGCATTGT TCTATTAGAA 
TGGGTTGAAC CAATGCAGAA ATTTATGTTA ATTAAGCACA TAGATTTTCC TATACCATGT 
CCACTTAGAA TGTTTGAAAT GCTGGTAGTT CCTGAACAGG AGTACCCTTT AGTTTGTGTT 
GGTGTCAGTA GAGGTAGAGA CTTCAACCAA GTGGTTCGAT TTGAGACGGT CAATCCAAAT 
TCTACCTCTT CATGGTTTAC AGAATCAGAT ACCCCACAGA CAAATGTTAC TCATGTAACC 
CAACTGGAGA GAGATACCAT CCTTGTATGC TTGGACTGTT GTATAAAAAT AGTAAATCTC 
CAAGGAAGAT TAAAATCTAG CAGGAAATTG TCATCAGAAC TCACCTTTGA TTTCCAGATT 
GAATCAATAG TGTGCCTACA AGACAGTGTG CTAGCTTTCT GGAAACATGG AATGCAAGGT 
AGAAGTTTTA GATCTAATGA GGTAACACAA GAAATTTCAG ATAGCACAAG AATTTTCAGG 
CTGCTTGGAT CTGACAGGGT CGTGGTTTTG GAAAGTAGGC CAACTGATAA CCCCACAGCA 
AATAGCAATT TGTACATCCT GGCGGGTCAT GAAAACAGTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003609.2
CDS	326..3010
Translation	MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

Target ORF information:

RefSeq Version	NM_003618.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003618.3

ORF Insert Sequence:

ATGAACCCCG GCTTCGATTT GTCCCGCCGG AACCCGCAGG AGGACTTCGA GCTGATTCAG 
CGCATCGGCA GCGGCACCTA CGGCGACGTC TACAAGGCAC GGAATGTTAA CACTGGTGAA 
TTAGCAGCAA TTAAAGTAAT AAAATTGGAA CCAGGAGAAG ACTTTGCAGT TGTGCAGCAA 
GAAATTATTA TGATGAAAGA CTGTAAACAC CCAAATATTG TTGCTTATTT TGGAAGCTAT 
CTCAGGCGAG ATAAGCTTTG GATTTGCATG GAGTTTTGTG GAGGTGGTTC TTTACAGGAT 
ATTTATCACG TAACTGGACC TCTGTCAGAA CTGCAAATTG CATATGTTAG CAGAGAAACA 
CTGCAGGGAT TATATTATCT TCACAGTAAA GGAAAAATGC ACAGAGATAT AAAGGGAGCT 
AACATTCTAT TAACGGATAA TGGTCATGTG AAATTGGCTG ATTTTGGAGT ATCTGCACAG 
ATAACAGCTA CAATTGCCAA ACGGAAGTCT TTCATTGGCA CACCATATTG GATGGCTCCA 
GAAGTTGCAG CTGTTGAGAG GAAGGGGGGT TACAATCAAC TCTGTGATCT CTGGGCAGTG 
GGAATCACTG CCATAGAACT TGCAGAGCTT CAGCCTCCTA TGTTTGACTT ACACCCAATG 
AGAGCATTAT TTCTAATGAC AAAAAGCAAT TTTCAGCCTC CTAAACTAAA GGATAAAATG 
AAATGGTCAA ATAGTTTTCA TCACTTTGTG AAAATGGCAC TTACCAAAAA TCCGAAAAAA 
AGACCTACTG CTGAAAAATT ATTACAGCAT CCTTTTGTAA CACAACATTT GACACGGTCT 
TTGGCAATCG AGCTGTTGGA TAAAGTAAAT AATCCAGATC ATTCCACTTA CCATGATTTC 
GATGATGATG ATCCTGAGCC TCTTGTTGCT GTACCACATA GAATTCACTC AACAAGTAGA 
AACGTGAGAG AAGAAAAAAC ACGCTCAGAG ATAACCTTTG GCCAAGTGAA ATTTGATCCA 
CCCTTAAGAA AGGAGACAGA ACCACATCAT GAACTTCCCG ACAGTGATGG TTTTTTGGAC 
AGTTCAGAAG AAATATACTA CACTGCAAGA TCTAATCTGG ATCTGCAACT GGAATATGGA 
CAAGGACACC AAGGTGGTTA CTTTTTAGGT GCAAACAAGA GTCTTCTCAA GTCTGTTGAA 
GAAGAATTGC ATCAGCGAGG ACACGTCGCA CATTTAGAAG ATGATGAAGG AGATGATGAT 
GAATCTAAAC ACTCAACTCT GAAAGCAAAA ATTCCACCTC CTTTGCCACC AAAGCCTAAG 
TCTATCTTCA TACCACAGGA AATGCATTCT ACTGAGGATG AAAATCAAGG AACAATCAAG 
AGATGTCCCA TGTCAGGGAG CCCAGCAAAG CCATCCCAAG TTCCACCTAG ACCACCACCT 
CCCAGATTAC CCCCACACAA ACCTGTTGCC TTAGGAAATG GAATGAGCTC CTTCCAGTTA 
AATGGTGAAC GAGATGGCTC ATTATGTCAA CAACAGAATG AACATAGAGG CACAAACCTT 
TCAAGAAAAG AAAAGAAAGA TGTACCAAAG CCTATTAGTA ATGGTCTTCC TCCAACACCT 
AAAGTGCATA TGGGTGCATG TTTTTCAAAA GTTTTTAATG GGTGTCCCTT GAAAATTCAC 
TGTGCATCAT CATGGATAAA CCCAGATACA AGAGATCAGT ACTTGATATT TGGTGCCGAA 
GAAGGGATTT ATACCCTCAA TCTTAATGAA CTTCATGAAA CATCAATGGA ACAGCTATTC 
CCTCGAAGGT GTACATGGTT GTATGTAATG AACAATTGCT TGCTATCAAT ATCTGGTAAA 
GCTTCTCAGC TTTATTCCCA TAATTTACCA GGGCTTTTTG ATTATGCAAG ACAAATGCAA 
AAGTTACCTG TTGCTATTCC AGCACACAAA CTCCCTGACA GAATACTGCC AAGGAAATTT 
TCTGTATCAG CAAAAATCCC TGAAACCAAA TGGTGCCAGA AGTGTTGTGT TGTAAGAAAT 
CCTTACACGG GCCATAAATA CCTATGTGGA GCACTTCAGA CTAGCATTGT TCTATTAGAA 
TGGGTTGAAC CAATGCAGAA ATTTATGTTA ATTAAGCACA TAGATTTTCC TATACCATGT 
CCACTTAGAA TGTTTGAAAT GCTGGTAGTT CCTGAACAGG AGTACCCTTT AGTTTGTGTT 
GGTGTCAGTA GAGGTAGAGA CTTCAACCAA GTGGTTCGAT TTGAGACGGT CAATCCAAAT 
TCTACCTCTT CATGGTTTAC AGAATCAGAT ACCCCACAGA CAAATGTTAC TCATGTAACC 
CAACTGGAGA GAGATACCAT CCTTGTATGC TTGGACTGTT GTATAAAAAT AGTAAATCTC 
CAAGGAAGAT TAAAATCTAG CAGGAAATTG TCATCAGAAC TCACCTTTGA TTTCCAGATT 
GAATCAATAG TGTGCCTACA AGACAGTGTG CTAGCTTTCT GGAAACATGG AATGCAAGGT 
AGAAGTTTTA GATCTAATGA GGTAACACAA GAAATTTCAG ATAGCACAAG AATTTTCAGG 
CTGCTTGGAT CTGACAGGGT CGTGGTTTTG GAAAGTAGGC CAACTGATAA CCCCACAGCA 
AATAGCAATT TGTACATCCT GGCGGGTCAT GAAAACAGTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Over-expression of miR-206 decreases the Euthyrox-resistance by targeting MAP4K3 in papillary thyroid carcinoma.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie114108605(2019 Jun)
Liu F,Yin R,Chen X,Chen W,Qian Y,Zhao Y,Jiang Y,Ma D,Hu T,Yu T,Zhu Y,Zhang Y

MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB.
Nature communications9(1)942(2018 03)
Hsu CL,Lee EX,Gordon KL,Paz EA,Shen WC,Ohnishi K,Meisenhelder J,Hunter T,La Spada AR

Germinal-center kinase-like kinase co-crystal structure reveals a swapped activation loop and C-terminal extension.
Protein science : a publication of the Protein Society26(2)152-162(2017 02)
Marcotte D,Rushe M,M Arduini R,Lukacs C,Atkins K,Sun X,Little K,Cullivan M,Paramasivam M,Patterson TA,Hesson T,D McKee T,May-Dracka TL,Xin Z,Bertolotti-Ciarlet A,Bhisetti GR,Lyssikatos JP,Silvian LF

MiR-199a-5p and let-7c cooperatively inhibit migration and invasion by targeting MAP4K3 in hepatocellular carcinoma.
Oncotarget8(8)13666-13677(2017 Feb)
Liu L,Lu L,Zheng A,Xie J,Xue Q,Wang F,Wang X,Zhou H,Tong X,Li Y,Zhu X,Wu G

Prediction of early hepatocellular carcinoma recurrence using germinal center kinase-like kinase.
Oncotarget7(31)49765-49776(2016 Aug)
Ho CH,Chuang HC,Wu IC,Tsai HW,Lin YJ,Sun HY,Young KC,Chiu YC,Cheng PN,Liu WC,Tan TH,Chang TT

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MAP4K3 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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