ADGRE3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADGRE3
Entrez Gene ID	84658
Full Name	adhesion G protein-coupled receptor E3
Synonyms	EMR3
General protein information	Preferred Names adhesion G protein-coupled receptor E3 Names adhesion G protein-coupled receptor E3 EGF-like module receptor 3 EGF-like module-containing mucin-like hormone receptor-like 3 egf-like module containing, mucin-like, hormone receptor-like 3 egf-like module-containing mucin-like receptor 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.12
Summary	This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014].
Disorder MIM:	606101

mRNA and Protein(s)

mRNA	Protein	Name
XM_011528374.2	XP_011526676.1	adhesion G protein-coupled receptor E3 isoform X1
NM_001289159.1	NP_001276088.1	adhesion G protein-coupled receptor E3 isoform 3 precursor
NM_032571.5	NP_115960.2	adhesion G protein-coupled receptor E3 isoform 1 precursor
NM_032571.4	NP_115960.2	adhesion G protein-coupled receptor E3 isoform 1 precursor
NM_001289158.1	NP_001276087.1	adhesion G protein-coupled receptor E3 isoform 2 precursor
NM_001289159.2	NP_001276088.1	adhesion G protein-coupled receptor E3 isoform 3 precursor
XM_017027383.1	XP_016882872.1	adhesion G protein-coupled receptor E3 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EMR3	XP_003316204.1
Homo sapiens (human)	EMR3	NP_115960.2
Canis lupus familiaris (dog)	EMR3	NP_001033755.1
Bos taurus (cattle)	EMR3	XP_005228688.1

Gene Ontology
Bibliography

Related articles in PubMed

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Hamann J, Aust G, Araç D, Engel FB, Formstone C, Fredriksson R, Hall RA, Harty BL, Kirchhoff C, Knapp B, Krishnan A, Liebscher I, Lin HH, Martinelli DC, Monk KR, Peeters MC, Piao X, Prömel S, Schöneberg T, Schwartz TW, Singer K, Stacey M, Ushkaryov YA, Vallon M, Wolfrum U, Wright MW, Xu L, Langenhan T, Schiöth HB
Pharmacological reviews67(2)338-67(2015)

EMR-3: a potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target.
Kane AJ, Sughrue ME, Rutkowski MJ, Phillips JJ, Parsa AT
Neuroreport21(16)1018-22(2010 Nov)

Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis.
Plager DA, Kahl JC, Asmann YW, Nilson AE, Pallanch JF, Friedman O, Kita H
PloS one5(7)e11450(2010 Jul)

New genetic associations detected in a host response study to hepatitis B vaccine.
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M
Genes and immunity11(3)232-8(2010 Apr)

The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes.
Matmati M, Pouwels W, van Bruggen R, Jansen M, Hoek RM, Verhoeven AJ, Hamann J
Journal of leukocyte biology81(2)440-8(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ADGRE3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRE3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu88772	XM_017027383.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu01156	NM_001289158.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu29077	NM_001289159.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu73662	XM_011528374.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu05054	NM_032571.5 Latest version!	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
View Old Accession Versions >>
OHu05054	NM_032571.4	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu29077	NM_001289159.1	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADGRE3 ( XM_017027383.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882872.1 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu88772
Clone ID Related Accession (Same CDS sequence)	XM_017027383.1
Accession Version	XM_017027383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor E3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAA TGGACATCCG TTGCAGTGAC ATCATCCAGG GAGACACACA AGGTCCCAGT 
GCCATTGCCT TTATCTCATA TTCTTCTCTT GGAAACATCA TAAATGCAAC TTTTTTTGAA 
GAGATGGATA AGAAAGATCA AGTGTATCTG AACTCTCAGG TTGTGAGTGC TGCTATTGGA 
CCCAAAAGGA ACGTGTCTCT CTCCAAGTCT GTGACGCTGA CTTTCCAGCA CGTGAAGATG 
ACCCCCAGTA CCAAAAAGGT CTTCTGTGTC TACTGGAAGA GCACAGGGCA GGGCAGCCAG 
TGGTCCAGGG ATGGCTGCTT CCTGATACAC GTGAACAAGA GTCACACCAT GTGTAATTGC 
AGTCACCTGT CCAGCTTCGC TGTCCTGATG GCCCTGACCA GCCAGGAGGA GGATCCCGTG 
CTGACTGTCA TCACCTACGT GGGGCTGAGC GTCTCTCTGC TGTGCCTCCT CCTGGCGGCC 
CTCACTTTTC TCCTGTGTAA AGCCATCCGG AACACCAGCA CCTCACTGCA TCTGCAGCTC 
TCGCTCTGCC TCTTCCTGGC CCACCTCCTC TTCCTCGTGG GGATTGATCG AACTGAACCC 
AAGGTGCTGT GCTCCATCAT CGCCGGTGCT TTGCACTATC TCTACCTGGC CGCCTTCACC 
TGGATGCTGC TGGAGGGTGT GCACCTCTTC CTCACTGCAC GGAACCTGAC AGTGGTCAAC 
TACTCAAGCA TCAATAGACT CATGAAGTGG ATCATGTTCC CAGTCGGCTA TGGCGTTCCC 
GCTGTGACTG TGGCCATTTC TGCAGCCTCC TGGCCTCACC TTTATGGAAC TGCTGATCGA 
TGCTGGCTCC ACCTGGACCA GGGATTCATG TGGAGTTTCC TTGGCCCAGT CTGTGCCATT 
TTCTCTGCGA ATTTAGTATT GTTTATCTTG GTCTTTTGGA TTTTGAAAAG AAAACTTTCC 
TCCCTCAATA GTGAAGTGTC AACCATCCAG AACACAAGGA TGCTGGCTTT CAAAGCAACA 
GCTCAGCTCT TCATCCTGGG CTGCACATGG TGTCTGGGCT TGCTACAGGT GGGTCCAGCT 
GCCCAGGTCA TGGCCTACCT CTTCACCATC ATCAACAGCC TCCAAGGCTT CTTCATCTTC 
TTGGTCTACT GCCTCCTCAG CCAGCAGGTC CAGAAACAAT ATCAAAAGTG GTTTAGAGAG 
ATCGTAAAAT CAAAATCTGA GTCTGAGACA TACACACTTT CCAGCAAGAT GGGTCCTGAC 
TCAAAACCCA GTGAGGGGGA TGTTTTTCCA GGACAAGTGA AGAGAAAATA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882872.1
CDS	88..1401
Translation	MNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

Target ORF information:

RefSeq Version	XM_017027383.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027383.1

ORF Insert Sequence:

ATGAACTCAA TGGACATCCG TTGCAGTGAC ATCATCCAGG GAGACACACA AGGTCCCAGT 
GCCATTGCCT TTATCTCATA TTCTTCTCTT GGAAACATCA TAAATGCAAC TTTTTTTGAA 
GAGATGGATA AGAAAGATCA AGTGTATCTG AACTCTCAGG TTGTGAGTGC TGCTATTGGA 
CCCAAAAGGA ACGTGTCTCT CTCCAAGTCT GTGACGCTGA CTTTCCAGCA CGTGAAGATG 
ACCCCCAGTA CCAAAAAGGT CTTCTGTGTC TACTGGAAGA GCACAGGGCA GGGCAGCCAG 
TGGTCCAGGG ATGGCTGCTT CCTGATACAC GTGAACAAGA GTCACACCAT GTGTAATTGC 
AGTCACCTGT CCAGCTTCGC TGTCCTGATG GCCCTGACCA GCCAGGAGGA GGATCCCGTG 
CTGACTGTCA TCACCTACGT GGGGCTGAGC GTCTCTCTGC TGTGCCTCCT CCTGGCGGCC 
CTCACTTTTC TCCTGTGTAA AGCCATCCGG AACACCAGCA CCTCACTGCA TCTGCAGCTC 
TCGCTCTGCC TCTTCCTGGC CCACCTCCTC TTCCTCGTGG GGATTGATCG AACTGAACCC 
AAGGTGCTGT GCTCCATCAT CGCCGGTGCT TTGCACTATC TCTACCTGGC CGCCTTCACC 
TGGATGCTGC TGGAGGGTGT GCACCTCTTC CTCACTGCAC GGAACCTGAC AGTGGTCAAC 
TACTCAAGCA TCAATAGACT CATGAAGTGG ATCATGTTCC CAGTCGGCTA TGGCGTTCCC 
GCTGTGACTG TGGCCATTTC TGCAGCCTCC TGGCCTCACC TTTATGGAAC TGCTGATCGA 
TGCTGGCTCC ACCTGGACCA GGGATTCATG TGGAGTTTCC TTGGCCCAGT CTGTGCCATT 
TTCTCTGCGA ATTTAGTATT GTTTATCTTG GTCTTTTGGA TTTTGAAAAG AAAACTTTCC 
TCCCTCAATA GTGAAGTGTC AACCATCCAG AACACAAGGA TGCTGGCTTT CAAAGCAACA 
GCTCAGCTCT TCATCCTGGG CTGCACATGG TGTCTGGGCT TGCTACAGGT GGGTCCAGCT 
GCCCAGGTCA TGGCCTACCT CTTCACCATC ATCAACAGCC TCCAAGGCTT CTTCATCTTC 
TTGGTCTACT GCCTCCTCAG CCAGCAGGTC CAGAAACAAT ATCAAAAGTG GTTTAGAGAG 
ATCGTAAAAT CAAAATCTGA GTCTGAGACA TACACACTTT CCAGCAAGAT GGGTCCTGAC 
TCAAAACCCA GTGAGGGGGA TGTTTTTCCA GGACAAGTGA AGAGAAAATA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRE3 ( NM_001289158.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276087.1 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01156
Clone ID Related Accession (Same CDS sequence)	NM_001289158.1
Accession Version	NM_001289158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor E3 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832663.1, AY358817.1, AF239764.1, BC128053.1 and AK298653.1. On Jan 16, 2014 this sequence version replaced XM_005260117.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY358817.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGA CACCACCTCC TCAAAGACAA CCGAGGGCAG GAAAGAGCTG 
CAAAAGATTG TGGACAAATT TGAGTCACTT CTCACCAATC AGACTTTATG GAGAACAGAA 
GGGAGACAAG AAATCTCATC CACAGCTACC ACTATTCTCC GGGATGTGGA ATCGAAAGTT 
CTAGAAACTG CCTTGAAAGA TCCAGAACAA AAAGTCCTGA AAATCCAAAA CGATAGTGTA 
GCTATTGAAA CTCAAGCGAT TACAGACAAT TGCTCTGAAG AAAGAAAGAC ATTCAACTTG 
AACGTCCAAA TGAACTCAAT GGACATCCGT TGCAGTGACA TCATCCAGGG AGACACACAA 
GGTCCCAGTG CCATTGCCTT TATCTCATAT TCTTCTCTTG GAAACATCAT AAATGCAACT 
TTTTTTGAAG AGATGGATAA GAAAGATCAA GTGTATCTGA ACTCTCAGGT TGTGAGTGCT 
GCTATTGGAC CCAAAAGGAA CGTGTCTCTC TCCAAGTCTG TGACGCTGAC TTTCCAGCAC 
GTGAAGATGA CCCCCAGTAC CAAAAAGGTC TTCTGTGTCT ACTGGAAGAG CACAGGGCAG 
GGCAGCCAGT GGTCCAGGGA TGGCTGCTTC CTGATACACG TGAACAAGAG TCACACCATG 
TGTAATTGCA GTCACCTGTC CAGCTTCGCT GTCCTGATGG CCCTGACCAG CCAGGAGGAG 
GATCCCGTGC TGACTGTCAT CACCTACGTG GGGCTGAGCG TCTCTCTGCT GTGCCTCCTC 
CTGGCGGCCC TCACTTTTCT CCTGTGTAAA GCCATCCGGA ACACCAGCAC CTCACTGCAT 
CTGCAGCTCT CGCTCTGCCT CTTCCTGGCC CACCTCCTCT TCCTCGTGGG GATTGATCGA 
ACTGAACCCA AGGTGCTGTG CTCCATCATC GCCGGTGCTT TGCACTATCT CTACCTGGCC 
GCCTTCACCT GGATGCTGCT GGAGGGTGTG CACCTCTTCC TCACTGCACG GAACCTGACA 
GTGGTCAACT ACTCAAGCAT CAATAGACTC ATGAAGTGGA TCATGTTCCC AGTCGGCTAT 
GGCGTTCCCG CTGTGACTGT GGCCATTTCT GCAGCCTCCT GGCCTCACCT TTATGGAACT 
GCTGATCGAT GCTGGCTCCA CCTGGACCAG GGATTCATGT GGAGTTTCCT TGGCCCAGTC 
TGTGCCATTT TCTCTGCGAA TTTAGTATTG TTTATCTTGG TCTTTTGGAT TTTGAAAAGA 
AAACTTTCCT CCCTCAATAG TGAAGTGTCA ACCATCCAGA ACACAAGGAT GCTGGCTTTC 
AAAGCAACAG CTCAGCTCTT CATCCTGGGC TGCACATGGT GTCTGGGCTT GCTACAGGTG 
GGTCCAGCTG CCCAGGTCAT GGCCTACCTC TTCACCATCA TCAACAGCCT CCAAGGCTTC 
TTCATCTTCT TGGTCTACTG CCTCCTCAGC CAGCAGGTCC AGAAACAATA TCAAAAGTGG 
TTTAGAGAGA TCGTAAAATC AAAATCTGAG TCTGAGACAT ACACACTTTC CAGCAAGATG 
GGTCCTGACT CAAAACCCAG TGAGGGGGAT GTTTTTCCAG GACAAGTGAA GAGAAAATAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276087.1
CDS	149..1951
Translation	MQGPLLLPGLCFLLSLFGAVTQKTKTSCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTFPLETCNDTTSSKTTEGRKELQKIVDKFESLLTNQTLWRTEGRQEISSTATTILRDVESKVLETALKDPEQKVLKIQNDSVAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

Target ORF information:

RefSeq Version	NM_001289158.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289158.1

ORF Insert Sequence:

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGA CACCACCTCC TCAAAGACAA CCGAGGGCAG GAAAGAGCTG 
CAAAAGATTG TGGACAAATT TGAGTCACTT CTCACCAATC AGACTTTATG GAGAACAGAA 
GGGAGACAAG AAATCTCATC CACAGCTACC ACTATTCTCC GGGATGTGGA ATCGAAAGTT 
CTAGAAACTG CCTTGAAAGA TCCAGAACAA AAAGTCCTGA AAATCCAAAA CGATAGTGTA 
GCTATTGAAA CTCAAGCGAT TACAGACAAT TGCTCTGAAG AAAGAAAGAC ATTCAACTTG 
AACGTCCAAA TGAACTCAAT GGACATCCGT TGCAGTGACA TCATCCAGGG AGACACACAA 
GGTCCCAGTG CCATTGCCTT TATCTCATAT TCTTCTCTTG GAAACATCAT AAATGCAACT 
TTTTTTGAAG AGATGGATAA GAAAGATCAA GTGTATCTGA ACTCTCAGGT TGTGAGTGCT 
GCTATTGGAC CCAAAAGGAA CGTGTCTCTC TCCAAGTCTG TGACGCTGAC TTTCCAGCAC 
GTGAAGATGA CCCCCAGTAC CAAAAAGGTC TTCTGTGTCT ACTGGAAGAG CACAGGGCAG 
GGCAGCCAGT GGTCCAGGGA TGGCTGCTTC CTGATACACG TGAACAAGAG TCACACCATG 
TGTAATTGCA GTCACCTGTC CAGCTTCGCT GTCCTGATGG CCCTGACCAG CCAGGAGGAG 
GATCCCGTGC TGACTGTCAT CACCTACGTG GGGCTGAGCG TCTCTCTGCT GTGCCTCCTC 
CTGGCGGCCC TCACTTTTCT CCTGTGTAAA GCCATCCGGA ACACCAGCAC CTCACTGCAT 
CTGCAGCTCT CGCTCTGCCT CTTCCTGGCC CACCTCCTCT TCCTCGTGGG GATTGATCGA 
ACTGAACCCA AGGTGCTGTG CTCCATCATC GCCGGTGCTT TGCACTATCT CTACCTGGCC 
GCCTTCACCT GGATGCTGCT GGAGGGTGTG CACCTCTTCC TCACTGCACG GAACCTGACA 
GTGGTCAACT ACTCAAGCAT CAATAGACTC ATGAAGTGGA TCATGTTCCC AGTCGGCTAT 
GGCGTTCCCG CTGTGACTGT GGCCATTTCT GCAGCCTCCT GGCCTCACCT TTATGGAACT 
GCTGATCGAT GCTGGCTCCA CCTGGACCAG GGATTCATGT GGAGTTTCCT TGGCCCAGTC 
TGTGCCATTT TCTCTGCGAA TTTAGTATTG TTTATCTTGG TCTTTTGGAT TTTGAAAAGA 
AAACTTTCCT CCCTCAATAG TGAAGTGTCA ACCATCCAGA ACACAAGGAT GCTGGCTTTC 
AAAGCAACAG CTCAGCTCTT CATCCTGGGC TGCACATGGT GTCTGGGCTT GCTACAGGTG 
GGTCCAGCTG CCCAGGTCAT GGCCTACCTC TTCACCATCA TCAACAGCCT CCAAGGCTTC 
TTCATCTTCT TGGTCTACTG CCTCCTCAGC CAGCAGGTCC AGAAACAATA TCAAAAGTGG 
TTTAGAGAGA TCGTAAAATC AAAATCTGAG TCTGAGACAT ACACACTTTC CAGCAAGATG 
GGTCCTGACT CAAAACCCAG TGAGGGGGAT GTTTTTCCAG GACAAGTGAA GAGAAAATAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRE3 ( NM_001289159.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276088.1 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu29077
Clone ID Related Accession (Same CDS sequence)	NM_001289159.1 , NM_001289159.2
Accession Version	NM_001289159.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor E3 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832663.1, AK298653.1, BC128053.1 and AF239764.1. On Jan 16, 2014 this sequence version replaced XM_005260119.1. Transcript Variant: This variant (3) lacks three alternate in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter isoform (3), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK298653.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGC TATTGAAACT CAAGCGATTA CAGACAATTG CTCTGAAGAA 
AGAAAGACAT TCAACTTGAA CGTCCAAATG AACTCAATGG ACATCCGTTG CAGTGACATC 
ATCCAGGGAG ACACACAAGG TCCCAGTGCC ATTGCCTTTA TCTCATATTC TTCTCTTGGA 
AACATCATAA ATGCAACTTT TTTTGAAGAG ATGGATAAGA AAGATCAAGT GTATCTGAAC 
TCTCAGGTTG TGAGTGCTGC TATTGGACCC AAAAGGAACG TGTCTCTCTC CAAGTCTGTG 
ACGCTGACTT TCCAGCACGT GAAGATGACC CCCAGTACCA AAAAGGTCTT CTGTGTCTAC 
TGGAAGAGCA CAGGGCAGGG CAGCCAGTGG TCCAGGGATG GCTGCTTCCT GATACACGTG 
AACAAGAGTC ACACCATGTG TAATTGCAGT CACCTGTCCA GCTTCGCTGT CCTGATGGCC 
CTGACCAGCC AGGAGGAGGA TCCCGTGCTG ACTGTCATCA CCTACGTGGG GCTGAGCGTC 
TCTCTGCTGT GCCTCCTCCT GGCGGCCCTC ACTTTTCTCC TGTGTAAAGC CATCCGGAAC 
ACCAGCACCT CACTGCATCT GCAGCTCTCG CTCTGCCTCT TCCTGGCCCA CCTCCTCTTC 
CTCGTGGGGA TTGATCGAAC TGAACCCAAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG 
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC 
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC 
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG 
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG 
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC 
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC 
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT 
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC 
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG 
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC 
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA 
CAAGTGAAGA GAAAATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276088.1
CDS	149..1729
Translation	MQGPLLLPGLCFLLSLFGAVTQKTKTSCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTFPLETCNAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

Target ORF information:

RefSeq Version	NM_001289159.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289159.1

ORF Insert Sequence:

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGC TATTGAAACT CAAGCGATTA CAGACAATTG CTCTGAAGAA 
AGAAAGACAT TCAACTTGAA CGTCCAAATG AACTCAATGG ACATCCGTTG CAGTGACATC 
ATCCAGGGAG ACACACAAGG TCCCAGTGCC ATTGCCTTTA TCTCATATTC TTCTCTTGGA 
AACATCATAA ATGCAACTTT TTTTGAAGAG ATGGATAAGA AAGATCAAGT GTATCTGAAC 
TCTCAGGTTG TGAGTGCTGC TATTGGACCC AAAAGGAACG TGTCTCTCTC CAAGTCTGTG 
ACGCTGACTT TCCAGCACGT GAAGATGACC CCCAGTACCA AAAAGGTCTT CTGTGTCTAC 
TGGAAGAGCA CAGGGCAGGG CAGCCAGTGG TCCAGGGATG GCTGCTTCCT GATACACGTG 
AACAAGAGTC ACACCATGTG TAATTGCAGT CACCTGTCCA GCTTCGCTGT CCTGATGGCC 
CTGACCAGCC AGGAGGAGGA TCCCGTGCTG ACTGTCATCA CCTACGTGGG GCTGAGCGTC 
TCTCTGCTGT GCCTCCTCCT GGCGGCCCTC ACTTTTCTCC TGTGTAAAGC CATCCGGAAC 
ACCAGCACCT CACTGCATCT GCAGCTCTCG CTCTGCCTCT TCCTGGCCCA CCTCCTCTTC 
CTCGTGGGGA TTGATCGAAC TGAACCCAAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG 
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC 
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC 
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG 
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG 
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC 
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC 
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT 
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC 
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG 
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC 
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA 
CAAGTGAAGA GAAAATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRE3 ( NM_001289159.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276088.1 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA.

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CloneID	OHu29077
Clone ID Related Accession (Same CDS sequence)	NM_001289159.1 , NM_001289159.2
Accession Version	NM_001289159.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559145600000
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor E3 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298653.1, BC128053.1 and AF239764.1. On May 31, 2019 this sequence version replaced NM_001289159.1. Transcript Variant: This variant (3) lacks three alternate in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter isoform (3), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK298653.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGC TATTGAAACT CAAGCGATTA CAGACAATTG CTCTGAAGAA 
AGAAAGACAT TCAACTTGAA CGTCCAAATG AACTCAATGG ACATCCGTTG CAGTGACATC 
ATCCAGGGAG ACACACAAGG TCCCAGTGCC ATTGCCTTTA TCTCATATTC TTCTCTTGGA 
AACATCATAA ATGCAACTTT TTTTGAAGAG ATGGATAAGA AAGATCAAGT GTATCTGAAC 
TCTCAGGTTG TGAGTGCTGC TATTGGACCC AAAAGGAACG TGTCTCTCTC CAAGTCTGTG 
ACGCTGACTT TCCAGCACGT GAAGATGACC CCCAGTACCA AAAAGGTCTT CTGTGTCTAC 
TGGAAGAGCA CAGGGCAGGG CAGCCAGTGG TCCAGGGATG GCTGCTTCCT GATACACGTG 
AACAAGAGTC ACACCATGTG TAATTGCAGT CACCTGTCCA GCTTCGCTGT CCTGATGGCC 
CTGACCAGCC AGGAGGAGGA TCCCGTGCTG ACTGTCATCA CCTACGTGGG GCTGAGCGTC 
TCTCTGCTGT GCCTCCTCCT GGCGGCCCTC ACTTTTCTCC TGTGTAAAGC CATCCGGAAC 
ACCAGCACCT CACTGCATCT GCAGCTCTCG CTCTGCCTCT TCCTGGCCCA CCTCCTCTTC 
CTCGTGGGGA TTGATCGAAC TGAACCCAAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG 
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC 
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC 
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG 
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG 
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC 
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC 
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT 
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC 
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG 
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC 
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA 
CAAGTGAAGA GAAAATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276088.1
CDS	75..1655
Translation	MQGPLLLPGLCFLLSLFGAVTQKTKTSCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTFPLETCNAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

Target ORF information:

RefSeq Version	NM_001289159.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289159.2

ORF Insert Sequence:

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGC TATTGAAACT CAAGCGATTA CAGACAATTG CTCTGAAGAA 
AGAAAGACAT TCAACTTGAA CGTCCAAATG AACTCAATGG ACATCCGTTG CAGTGACATC 
ATCCAGGGAG ACACACAAGG TCCCAGTGCC ATTGCCTTTA TCTCATATTC TTCTCTTGGA 
AACATCATAA ATGCAACTTT TTTTGAAGAG ATGGATAAGA AAGATCAAGT GTATCTGAAC 
TCTCAGGTTG TGAGTGCTGC TATTGGACCC AAAAGGAACG TGTCTCTCTC CAAGTCTGTG 
ACGCTGACTT TCCAGCACGT GAAGATGACC CCCAGTACCA AAAAGGTCTT CTGTGTCTAC 
TGGAAGAGCA CAGGGCAGGG CAGCCAGTGG TCCAGGGATG GCTGCTTCCT GATACACGTG 
AACAAGAGTC ACACCATGTG TAATTGCAGT CACCTGTCCA GCTTCGCTGT CCTGATGGCC 
CTGACCAGCC AGGAGGAGGA TCCCGTGCTG ACTGTCATCA CCTACGTGGG GCTGAGCGTC 
TCTCTGCTGT GCCTCCTCCT GGCGGCCCTC ACTTTTCTCC TGTGTAAAGC CATCCGGAAC 
ACCAGCACCT CACTGCATCT GCAGCTCTCG CTCTGCCTCT TCCTGGCCCA CCTCCTCTTC 
CTCGTGGGGA TTGATCGAAC TGAACCCAAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG 
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC 
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC 
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG 
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG 
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC 
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC 
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT 
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC 
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG 
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC 
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA 
CAAGTGAAGA GAAAATATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRE3 ( XM_011528374.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526676.1 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X2, mRNA.

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CloneID	OHu73662
Clone ID Related Accession (Same CDS sequence)	XM_011528374.2
Accession Version	XM_011528374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor E3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGGAC CATTGCTTCT TCCAGCTTCC TGTGCTAAGT GCCCCCCAAA TGCTTCCTGT 
GTCAATAACA CTCACTGCAC CTGCAACCAT GGATATACTT CTGGATCTGG GCAGAAACTA 
TTCACATTCC CCTTGGAGAC ATGTAACGAC ATTAATGAAT GTACACCACC CTATAGTGTA 
TATTGTGGAT TTAACGCTGT GTGTTACAAT GTCGAAGGAA GTTTCTACTG TCAATGTGTC 
CCAGGATATA GACTGCATTC TGGGAATGAA CAATTCAGTA ATTCCAATGA GAACACCTGT 
CAGGACACCA CCTCCTCAAA GACAACCGAG GGCAGGAAAG AGCTGCAAAA GATTGTGGAC 
AAATTTGAGT CACTTCTCAC CAATCAGACT TTATGGAGAA CAGAAGGGAG ACAAGAAATC 
TCATCCACAG CTACCACTAT TCTCCGGGAT GTGGAATCGA AAGTTCTAGA AACTGCCTTG 
AAAGATCCAG AACAAAAAGT CCTGAAAATC CAAAACGATA GTGTAGCTAT TGAAACTCAA 
GCGATTACAG ACAATTGCTC TGAAGAAAGA AAGACATTCA ACTTGAACGT CCAAATGAAC 
TCAATGGACA TCCGTTGCAG TGACATCATC CAGGGAGACA CACAAGGTCC CAGTGCCATT 
GCCTTTATCT CATATTCTTC TCTTGGAAAC ATCATAAATG CAACTTTTTT TGAAGAGATG 
GATAAGAAAG ATCAAGTGTA TCTGAACTCT CAGGTTGTGA GTGCTGCTAT TGGACCCAAA 
AGGAACGTGT CTCTCTCCAA GTCTGTGACG CTGACTTTCC AGCACGTGAA GATGACCCCC 
AGTACCAAAA AGGTCTTCTG TGTCTACTGG AAGAGCACAG GGCAGGGCAG CCAGTGGTCC 
AGGGATGGCT GCTTCCTGAT ACACGTGAAC AAGAGTCACA CCATGTGTAA TTGCAGTCAC 
CTGTCCAGCT TCGCTGTCCT GATGGCCCTG ACCAGCCAGG AGGAGGATCC CGTGCTGACT 
GTCATCACCT ACGTGGGGCT GAGCGTCTCT CTGCTGTGCC TCCTCCTGGC GGCCCTCACT 
TTTCTCCTGT GTAAAGCCAT CCGGAACACC AGCACCTCAC TGCATCTGCA GCTCTCGCTC 
TGCCTCTTCC TGGCCCACCT CCTCTTCCTC GTGGGGATTG ATCGAACTGA ACCCAAGGTG 
CTGTGCTCCA TCATCGCCGG TGCTTTGCAC TATCTCTACC TGGCCGCCTT CACCTGGATG 
CTGCTGGAGG GTGTGCACCT CTTCCTCACT GCACGGAACC TGACAGTGGT CAACTACTCA 
AGCATCAATA GACTCATGAA GTGGATCATG TTCCCAGTCG GCTATGGCGT TCCCGCTGTG 
ACTGTGGCCA TTTCTGCAGC CTCCTGGCCT CACCTTTATG GAACTGCTGA TCGATGCTGG 
CTCCACCTGG ACCAGGGATT CATGTGGAGT TTCCTTGGCC CAGTCTGTGC CATTTTCTCT 
GCGAATTTAG TATTGTTTAT CTTGGTCTTT TGGATTTTGA AAAGAAAACT TTCCTCCCTC 
AATAGTGAAG TGTCAACCAT CCAGAACACA AGGATGCTGG CTTTCAAAGC AACAGCTCAG 
CTCTTCATCC TGGGCTGCAC ATGGTGTCTG GGCTTGCTAC AGGTGGGTCC AGCTGCCCAG 
GTCATGGCCT ACCTCTTCAC CATCATCAAC AGCCTCCAAG GCTTCTTCAT CTTCTTGGTC 
TACTGCCTCC TCAGCCAGCA GGTCCAGAAA CAATATCAAA AGTGGTTTAG AGAGATCGTA 
AAATCAAAAT CTGAGTCTGA GACATACACA CTTTCCAGCA AGATGGGTCC TGACTCAAAA 
CCCAGTGAGG GGGATGTTTT TCCAGGACAA GTGAAGAGAA AATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526676.1
CDS	91..1998
Translation	MQGPLLLPASCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTFPLETCNDINECTPPYSVYCGFNAVCYNVEGSFYCQCVPGYRLHSGNEQFSNSNENTCQDTTSSKTTEGRKELQKIVDKFESLLTNQTLWRTEGRQEISSTATTILRDVESKVLETALKDPEQKVLKIQNDSVAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

Target ORF information:

RefSeq Version	XM_011528374.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528374.2

ORF Insert Sequence:

ATGCAGGGAC CATTGCTTCT TCCAGCTTCC TGTGCTAAGT GCCCCCCAAA TGCTTCCTGT 
GTCAATAACA CTCACTGCAC CTGCAACCAT GGATATACTT CTGGATCTGG GCAGAAACTA 
TTCACATTCC CCTTGGAGAC ATGTAACGAC ATTAATGAAT GTACACCACC CTATAGTGTA 
TATTGTGGAT TTAACGCTGT GTGTTACAAT GTCGAAGGAA GTTTCTACTG TCAATGTGTC 
CCAGGATATA GACTGCATTC TGGGAATGAA CAATTCAGTA ATTCCAATGA GAACACCTGT 
CAGGACACCA CCTCCTCAAA GACAACCGAG GGCAGGAAAG AGCTGCAAAA GATTGTGGAC 
AAATTTGAGT CACTTCTCAC CAATCAGACT TTATGGAGAA CAGAAGGGAG ACAAGAAATC 
TCATCCACAG CTACCACTAT TCTCCGGGAT GTGGAATCGA AAGTTCTAGA AACTGCCTTG 
AAAGATCCAG AACAAAAAGT CCTGAAAATC CAAAACGATA GTGTAGCTAT TGAAACTCAA 
GCGATTACAG ACAATTGCTC TGAAGAAAGA AAGACATTCA ACTTGAACGT CCAAATGAAC 
TCAATGGACA TCCGTTGCAG TGACATCATC CAGGGAGACA CACAAGGTCC CAGTGCCATT 
GCCTTTATCT CATATTCTTC TCTTGGAAAC ATCATAAATG CAACTTTTTT TGAAGAGATG 
GATAAGAAAG ATCAAGTGTA TCTGAACTCT CAGGTTGTGA GTGCTGCTAT TGGACCCAAA 
AGGAACGTGT CTCTCTCCAA GTCTGTGACG CTGACTTTCC AGCACGTGAA GATGACCCCC 
AGTACCAAAA AGGTCTTCTG TGTCTACTGG AAGAGCACAG GGCAGGGCAG CCAGTGGTCC 
AGGGATGGCT GCTTCCTGAT ACACGTGAAC AAGAGTCACA CCATGTGTAA TTGCAGTCAC 
CTGTCCAGCT TCGCTGTCCT GATGGCCCTG ACCAGCCAGG AGGAGGATCC CGTGCTGACT 
GTCATCACCT ACGTGGGGCT GAGCGTCTCT CTGCTGTGCC TCCTCCTGGC GGCCCTCACT 
TTTCTCCTGT GTAAAGCCAT CCGGAACACC AGCACCTCAC TGCATCTGCA GCTCTCGCTC 
TGCCTCTTCC TGGCCCACCT CCTCTTCCTC GTGGGGATTG ATCGAACTGA ACCCAAGGTG 
CTGTGCTCCA TCATCGCCGG TGCTTTGCAC TATCTCTACC TGGCCGCCTT CACCTGGATG 
CTGCTGGAGG GTGTGCACCT CTTCCTCACT GCACGGAACC TGACAGTGGT CAACTACTCA 
AGCATCAATA GACTCATGAA GTGGATCATG TTCCCAGTCG GCTATGGCGT TCCCGCTGTG 
ACTGTGGCCA TTTCTGCAGC CTCCTGGCCT CACCTTTATG GAACTGCTGA TCGATGCTGG 
CTCCACCTGG ACCAGGGATT CATGTGGAGT TTCCTTGGCC CAGTCTGTGC CATTTTCTCT 
GCGAATTTAG TATTGTTTAT CTTGGTCTTT TGGATTTTGA AAAGAAAACT TTCCTCCCTC 
AATAGTGAAG TGTCAACCAT CCAGAACACA AGGATGCTGG CTTTCAAAGC AACAGCTCAG 
CTCTTCATCC TGGGCTGCAC ATGGTGTCTG GGCTTGCTAC AGGTGGGTCC AGCTGCCCAG 
GTCATGGCCT ACCTCTTCAC CATCATCAAC AGCCTCCAAG GCTTCTTCAT CTTCTTGGTC 
TACTGCCTCC TCAGCCAGCA GGTCCAGAAA CAATATCAAA AGTGGTTTAG AGAGATCGTA 
AAATCAAAAT CTGAGTCTGA GACATACACA CTTTCCAGCA AGATGGGTCC TGACTCAAAA 
CCCAGTGAGG GGGATGTTTT TCCAGGACAA GTGAAGAGAA AATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRE3 ( NM_032571.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_115960.2 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA.

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CloneID	OHu05054
Clone ID Related Accession (Same CDS sequence)	NM_032571.5 , NM_032571.4
Accession Version	NM_032571.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571500800000
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor E3 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832663.1, BC121130.2, AF239764.1, BC128053.1 and AK298653.1. On May 17, 2019 this sequence version replaced NM_032571.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF239764.1, AK290909.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253673.6/ ENSP00000253673.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA 
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT 
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC 
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG 
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA 
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA 
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA 
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC 
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC 
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA 
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG 
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA 
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC 
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC 
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GAGGAGGATC CCGTGCTGAC TGTCATCACC 
TACGTGGGGC TGAGCGTCTC TCTGCTGTGC CTCCTCCTGG CGGCCCTCAC TTTTCTCCTG 
TGTAAAGCCA TCCGGAACAC CAGCACCTCA CTGCATCTGC AGCTCTCGCT CTGCCTCTTC 
CTGGCCCACC TCCTCTTCCT CGTGGGGATT GATCGAACTG AACCCAAGGT GCTGTGCTCC 
ATCATCGCCG GTGCTTTGCA CTATCTCTAC CTGGCCGCCT TCACCTGGAT GCTGCTGGAG 
GGTGTGCACC TCTTCCTCAC TGCACGGAAC CTGACAGTGG TCAACTACTC AAGCATCAAT 
AGACTCATGA AGTGGATCAT GTTCCCAGTC GGCTATGGCG TTCCCGCTGT GACTGTGGCC 
ATTTCTGCAG CCTCCTGGCC TCACCTTTAT GGAACTGCTG ATCGATGCTG GCTCCACCTG 
GACCAGGGAT TCATGTGGAG TTTCCTTGGC CCAGTCTGTG CCATTTTCTC TGCGAATTTA 
GTATTGTTTA TCTTGGTCTT TTGGATTTTG AAAAGAAAAC TTTCCTCCCT CAATAGTGAA 
GTGTCAACCA TCCAGAACAC AAGGATGCTG GCTTTCAAAG CAACAGCTCA GCTCTTCATC 
CTGGGCTGCA CATGGTGTCT GGGCTTGCTA CAGGTGGGTC CAGCTGCCCA GGTCATGGCC 
TACCTCTTCA CCATCATCAA CAGCCTCCAA GGCTTCTTCA TCTTCTTGGT CTACTGCCTC 
CTCAGCCAGC AGGTCCAGAA ACAATATCAA AAGTGGTTTA GAGAGATCGT AAAATCAAAA 
TCTGAGTCTG AGACATACAC ACTTTCCAGC AAGATGGGTC CTGACTCAAA ACCCAGTGAG 
GGGGATGTTT TTCCAGGACA AGTGAAGAGA AAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115960.2
CDS	75..2033
Translation	MQGPLLLPGLCFLLSLFGAVTQKTKTSCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTFPLETCNDINECTPPYSVYCGFNAVCYNVEGSFYCQCVPGYRLHSGNEQFSNSNENTCQDTTSSKTTEGRKELQKIVDKFESLLTNQTLWRTEGRQEISSTATTILRDVESKVLETALKDPEQKVLKIQNDSVAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

Target ORF information:

RefSeq Version	NM_032571.5
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032571.5

ORF Insert Sequence:

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA 
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT 
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC 
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG 
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA 
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA 
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA 
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC 
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC 
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA 
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG 
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA 
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC 
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC 
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GAGGAGGATC CCGTGCTGAC TGTCATCACC 
TACGTGGGGC TGAGCGTCTC TCTGCTGTGC CTCCTCCTGG CGGCCCTCAC TTTTCTCCTG 
TGTAAAGCCA TCCGGAACAC CAGCACCTCA CTGCATCTGC AGCTCTCGCT CTGCCTCTTC 
CTGGCCCACC TCCTCTTCCT CGTGGGGATT GATCGAACTG AACCCAAGGT GCTGTGCTCC 
ATCATCGCCG GTGCTTTGCA CTATCTCTAC CTGGCCGCCT TCACCTGGAT GCTGCTGGAG 
GGTGTGCACC TCTTCCTCAC TGCACGGAAC CTGACAGTGG TCAACTACTC AAGCATCAAT 
AGACTCATGA AGTGGATCAT GTTCCCAGTC GGCTATGGCG TTCCCGCTGT GACTGTGGCC 
ATTTCTGCAG CCTCCTGGCC TCACCTTTAT GGAACTGCTG ATCGATGCTG GCTCCACCTG 
GACCAGGGAT TCATGTGGAG TTTCCTTGGC CCAGTCTGTG CCATTTTCTC TGCGAATTTA 
GTATTGTTTA TCTTGGTCTT TTGGATTTTG AAAAGAAAAC TTTCCTCCCT CAATAGTGAA 
GTGTCAACCA TCCAGAACAC AAGGATGCTG GCTTTCAAAG CAACAGCTCA GCTCTTCATC 
CTGGGCTGCA CATGGTGTCT GGGCTTGCTA CAGGTGGGTC CAGCTGCCCA GGTCATGGCC 
TACCTCTTCA CCATCATCAA CAGCCTCCAA GGCTTCTTCA TCTTCTTGGT CTACTGCCTC 
CTCAGCCAGC AGGTCCAGAA ACAATATCAA AAGTGGTTTA GAGAGATCGT AAAATCAAAA 
TCTGAGTCTG AGACATACAC ACTTTCCAGC AAGATGGGTC CTGACTCAAA ACCCAGTGAG 
GGGGATGTTT TTCCAGGACA AGTGAAGAGA AAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRE3 ( NM_032571.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115960.2 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA.

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CloneID	OHu05054
Clone ID Related Accession (Same CDS sequence)	NM_032571.5 , NM_032571.4
Accession Version	NM_032571.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor E3 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832663.1, BC121130.2, AF239764.1, BC128053.1 and AK298653.1. On Jan 16, 2014 this sequence version replaced NM_032571.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC142639.1, AF239764.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA 
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT 
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC 
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG 
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA 
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA 
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA 
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC 
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC 
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA 
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG 
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA 
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC 
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC 
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GAGGAGGATC CCGTGCTGAC TGTCATCACC 
TACGTGGGGC TGAGCGTCTC TCTGCTGTGC CTCCTCCTGG CGGCCCTCAC TTTTCTCCTG 
TGTAAAGCCA TCCGGAACAC CAGCACCTCA CTGCATCTGC AGCTCTCGCT CTGCCTCTTC 
CTGGCCCACC TCCTCTTCCT CGTGGGGATT GATCGAACTG AACCCAAGGT GCTGTGCTCC 
ATCATCGCCG GTGCTTTGCA CTATCTCTAC CTGGCCGCCT TCACCTGGAT GCTGCTGGAG 
GGTGTGCACC TCTTCCTCAC TGCACGGAAC CTGACAGTGG TCAACTACTC AAGCATCAAT 
AGACTCATGA AGTGGATCAT GTTCCCAGTC GGCTATGGCG TTCCCGCTGT GACTGTGGCC 
ATTTCTGCAG CCTCCTGGCC TCACCTTTAT GGAACTGCTG ATCGATGCTG GCTCCACCTG 
GACCAGGGAT TCATGTGGAG TTTCCTTGGC CCAGTCTGTG CCATTTTCTC TGCGAATTTA 
GTATTGTTTA TCTTGGTCTT TTGGATTTTG AAAAGAAAAC TTTCCTCCCT CAATAGTGAA 
GTGTCAACCA TCCAGAACAC AAGGATGCTG GCTTTCAAAG CAACAGCTCA GCTCTTCATC 
CTGGGCTGCA CATGGTGTCT GGGCTTGCTA CAGGTGGGTC CAGCTGCCCA GGTCATGGCC 
TACCTCTTCA CCATCATCAA CAGCCTCCAA GGCTTCTTCA TCTTCTTGGT CTACTGCCTC 
CTCAGCCAGC AGGTCCAGAA ACAATATCAA AAGTGGTTTA GAGAGATCGT AAAATCAAAA 
TCTGAGTCTG AGACATACAC ACTTTCCAGC AAGATGGGTC CTGACTCAAA ACCCAGTGAG 
GGGGATGTTT TTCCAGGACA AGTGAAGAGA AAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115960.2
CDS	149..2107
Translation	MQGPLLLPGLCFLLSLFGAVTQKTKTSCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTFPLETCNDINECTPPYSVYCGFNAVCYNVEGSFYCQCVPGYRLHSGNEQFSNSNENTCQDTTSSKTTEGRKELQKIVDKFESLLTNQTLWRTEGRQEISSTATTILRDVESKVLETALKDPEQKVLKIQNDSVAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFISYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTKKVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVITYVGLSVSLLCLLLAALTFLLCKAIRNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCSIIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVAISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSEVSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCLLSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

Target ORF information:

RefSeq Version	NM_032571.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032571.4

ORF Insert Sequence:

ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC 
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC 
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA 
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT 
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC 
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG 
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA 
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA 
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA 
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC 
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC 
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA 
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG 
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA 
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC 
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC 
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GAGGAGGATC CCGTGCTGAC TGTCATCACC 
TACGTGGGGC TGAGCGTCTC TCTGCTGTGC CTCCTCCTGG CGGCCCTCAC TTTTCTCCTG 
TGTAAAGCCA TCCGGAACAC CAGCACCTCA CTGCATCTGC AGCTCTCGCT CTGCCTCTTC 
CTGGCCCACC TCCTCTTCCT CGTGGGGATT GATCGAACTG AACCCAAGGT GCTGTGCTCC 
ATCATCGCCG GTGCTTTGCA CTATCTCTAC CTGGCCGCCT TCACCTGGAT GCTGCTGGAG 
GGTGTGCACC TCTTCCTCAC TGCACGGAAC CTGACAGTGG TCAACTACTC AAGCATCAAT 
AGACTCATGA AGTGGATCAT GTTCCCAGTC GGCTATGGCG TTCCCGCTGT GACTGTGGCC 
ATTTCTGCAG CCTCCTGGCC TCACCTTTAT GGAACTGCTG ATCGATGCTG GCTCCACCTG 
GACCAGGGAT TCATGTGGAG TTTCCTTGGC CCAGTCTGTG CCATTTTCTC TGCGAATTTA 
GTATTGTTTA TCTTGGTCTT TTGGATTTTG AAAAGAAAAC TTTCCTCCCT CAATAGTGAA 
GTGTCAACCA TCCAGAACAC AAGGATGCTG GCTTTCAAAG CAACAGCTCA GCTCTTCATC 
CTGGGCTGCA CATGGTGTCT GGGCTTGCTA CAGGTGGGTC CAGCTGCCCA GGTCATGGCC 
TACCTCTTCA CCATCATCAA CAGCCTCCAA GGCTTCTTCA TCTTCTTGGT CTACTGCCTC 
CTCAGCCAGC AGGTCCAGAA ACAATATCAA AAGTGGTTTA GAGAGATCGT AAAATCAAAA 
TCTGAGTCTG AGACATACAC ACTTTCCAGC AAGATGGGTC CTGACTCAAA ACCCAGTGAG 
GGGGATGTTT TTCCAGGACA AGTGAAGAGA AAATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Pharmacological reviews67(2)338-67(2015)
Hamann J,Aust G,Araç D,Engel FB,Formstone C,Fredriksson R,Hall RA,Harty BL,Kirchhoff C,Knapp B,Krishnan A,Liebscher I,Lin HH,Martinelli DC,Monk KR,Peeters MC,Piao X,Prömel S,Schöneberg T,Schwartz TW,Singer K,Stacey M,Ushkaryov YA,Vallon M,Wolfrum U,Wright MW,Xu L,Langenhan T,Schiöth HB

EMR-3: a potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target.
Neuroreport21(16)1018-22(2010 Nov)
Kane AJ,Sughrue ME,Rutkowski MJ,Phillips JJ,Parsa AT

Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis.
PloS one5(7)e11450(2010 Jul)
Plager DA,Kahl JC,Asmann YW,Nilson AE,Pallanch JF,Friedman O,Kita H

New genetic associations detected in a host response study to hepatitis B vaccine.
Genes and immunity11(3)232-8(2010 Apr)
Davila S,Froeling FE,Tan A,Bonnard C,Boland GJ,Snippe H,Hibberd ML,Seielstad M

The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes.
Journal of leukocyte biology81(2)440-8(2007 Feb)
Matmati M,Pouwels W,van Bruggen R,Jansen M,Hoek RM,Verhoeven AJ,Hamann J

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ADGRE3 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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