COL25A1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011532335.2	XP_011530637.1	collagen alpha-1(XXV) chain isoform X1
XM_011532338.2	XP_011530640.1	collagen alpha-1(XXV) chain isoform X2
XM_017008736.1	XP_016864225.1	collagen alpha-1(XXV) chain isoform X4
XM_011532334.2	XP_011530636.1	collagen alpha-1(XXV) chain isoform X1
NM_198721.4	NP_942014.1	collagen alpha-1(XXV) chain isoform 1
NM_032518.3	NP_115907.2	collagen alpha-1(XXV) chain isoform 2
NM_001256074.1	NP_001243003.1	collagen alpha-1(XXV) chain isoform 3
NM_198721.3	NP_942014.1	collagen alpha-1(XXV) chain isoform 1
XM_017008735.1	XP_016864224.1	collagen alpha-1(XXV) chain isoform X3
NM_032518.2	NP_115907.2	collagen alpha-1(XXV) chain isoform 2
XM_011532333.2	XP_011530635.1	collagen alpha-1(XXV) chain isoform X1
XM_011532356.2	XP_011530658.1	collagen alpha-1(XXV) chain isoform X6
XM_011532358.2	XP_011530660.1	collagen alpha-1(XXV) chain isoform X7
XM_011532355.2	XP_011530657.1	collagen alpha-1(XXV) chain isoform X5
XM_017008737.1	XP_016864226.1	collagen alpha-1(XXV) chain isoform X5
NM_001256074.2	NP_001243003.1	collagen alpha-1(XXV) chain isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11177	NM_001256074.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu73579	XM_011532333.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu73579	XM_011532335.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu73579	XM_011532334.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu73582	XM_011532338.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu73599	XM_017008737.1 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu73599	XM_011532355.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu73600	XM_011532356.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu73602	XM_011532358.2 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu95483	XM_017008735.1 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu95484	XM_017008736.1 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu02085	NM_032518.3 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu11928	NM_198721.4 Latest version!	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$307.30 $439.00
View Old Accession Versions >>
OHu11928	NM_198721.3	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$307.30 $439.00
OHu02085	NM_032518.2	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu11177	NM_001256074.1	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

COL25A1 ( NM_001256074.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243003.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11177
Clone ID Related Accession (Same CDS sequence)	NM_001256074.1 , NM_001256074.2
Accession Version	NM_001256074.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549036800000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC030649.1, AC004051.1 and AC097473.3. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, and also differs in the 5' and 3' UTRs, compared to variant 1. The resulting isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGGGCCTGCC AGGCTTCCCC ACAGTTGCTG CCCTGCACAG TAATCAGATC 
CTCACCGTCA AGGGTGACCA AGGACAGGCA GGGCCTCCAG GACCCCCTGG CCCTCCAGGC 
CCAAGAGGGC CACCTGGGGA CACAGGGAAA GATGGCCCCC GTGGAATGCC AGGAGTACCC 
GGTGAACCAG GAAAGCCAGG AGAACAAGGC TTGATGGGTC CTCTAGGGCC TCCGGGACAA 
AAGGGTTCTA TTGGAGCACC TGGAATTCCA GGGATGAATG GGCAAAAGGG TGAGCCCGGG 
TTGCCTGGAG CAGTAGGACA GAATGGAATA CCAGGACCTA AGGGAGAACC TGGAGAACAA 
GGTGAAAAGG GAGACGCTGG AGAGAACGGC CCCAAGGGTG ACACAGGCGA AAAGGGTGAC 
CCTGGATCAT CTGCTGCAGG AATTAAGGGA GAACCTGGGG AATCTGGTCG TCCAGGGCAA 
AAGGGTGAAC CAGGGCTTCC TGGGCTTCCT GGACTTCCGG GGATAAAGGG AGAACCAGGT 
TTCATTGGTC CTCAAGGAGA ACCAGGCTTA CCAGGTTTAC CAGGAACAAA AGGTGAACGG 
GGGGAAGCAG GGCCTCCTGG AAGAGGTGAG CGAGGGGAAC CTGGAGCCCC CGGACCAAAG 
GGGGATCGTG GAGAAAAAGG GGACTCTGGA GCTCAGGGAC CAAGGGGTCC ACCTGGTCAA 
AAAGGGGATC AAGGAGCCAC TGAGATCATA GACTACAACG GCAACCTCCA CGAAGCCTTA 
CAGGGTCCCC CTGGACCTCC TGGACCTCAA GGACTACAAG GGCCAAAGGG AGAGCAGGGA 
TCTCCAGGAA TCCCAGGAAT GGATGGAGAG CAGGGACTCA AAGGCTCAAA GGGAGACATG 
GGGGACCCAG GTATGACAGG TGAAAAAGGA GGAATTGGAC TTCCTGGATT ACCGGGAGCC 
AATGGAATGA AAGGAGAAAA AGGAGATTCT GGAATGCCGG GTCCACAGGG TCCTTCTATC 
ATAGGCCCAC CAGGCCCACC AGGTCCCCAT GGCCCACCTG GCCCCATGGG ACCTCATGGA 
CTTCCTGGAC CAAAGGGTGA ACCAGGGTTA AATGGTGTTA AAGGATTGAA GGGTGAACCA 
GGTCAAAAGG GTGACAGAGG ACCCCTTGGT CTTCCAGGAG CATCTGGCTT AGACGGAAAG 
CCAGGATCCC GGGGTACAGA TGGTCCTATG GGACCCCATG GCCCTGCAGG TCCCAAAGGA 
GAAAGAGGTG AAAAAGGAGC TATGGGAGAG CCTGGACCAA GAGGGCCCTA TGGGCTGCCT 
GGCTTCCCTG GTCCACGGGG TGAGAAGGGT GATCTAGGAG AAAAGGGAGA AAAGGTGACC 
TCTCCATCCC AGCATGTACC TTGTTTGATT CTTCTCCTGC TGTCTGCTTT GCTGTTCAGC 
TTGTGTGATT CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243003.1
CDS	349..2286
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRGLPGFPTVAALHSNQILTVKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGFPGPRGEKGDLGEKGEKVTSPSQHVPCLILLLLSALLFSLCDSI

Target ORF information:

RefSeq Version	NM_001256074.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256074.1

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGGGCCTGCC AGGCTTCCCC ACAGTTGCTG CCCTGCACAG TAATCAGATC 
CTCACCGTCA AGGGTGACCA AGGACAGGCA GGGCCTCCAG GACCCCCTGG CCCTCCAGGC 
CCAAGAGGGC CACCTGGGGA CACAGGGAAA GATGGCCCCC GTGGAATGCC AGGAGTACCC 
GGTGAACCAG GAAAGCCAGG AGAACAAGGC TTGATGGGTC CTCTAGGGCC TCCGGGACAA 
AAGGGTTCTA TTGGAGCACC TGGAATTCCA GGGATGAATG GGCAAAAGGG TGAGCCCGGG 
TTGCCTGGAG CAGTAGGACA GAATGGAATA CCAGGACCTA AGGGAGAACC TGGAGAACAA 
GGTGAAAAGG GAGACGCTGG AGAGAACGGC CCCAAGGGTG ACACAGGCGA AAAGGGTGAC 
CCTGGATCAT CTGCTGCAGG AATTAAGGGA GAACCTGGGG AATCTGGTCG TCCAGGGCAA 
AAGGGTGAAC CAGGGCTTCC TGGGCTTCCT GGACTTCCGG GGATAAAGGG AGAACCAGGT 
TTCATTGGTC CTCAAGGAGA ACCAGGCTTA CCAGGTTTAC CAGGAACAAA AGGTGAACGG 
GGGGAAGCAG GGCCTCCTGG AAGAGGTGAG CGAGGGGAAC CTGGAGCCCC CGGACCAAAG 
GGGGATCGTG GAGAAAAAGG GGACTCTGGA GCTCAGGGAC CAAGGGGTCC ACCTGGTCAA 
AAAGGGGATC AAGGAGCCAC TGAGATCATA GACTACAACG GCAACCTCCA CGAAGCCTTA 
CAGGGTCCCC CTGGACCTCC TGGACCTCAA GGACTACAAG GGCCAAAGGG AGAGCAGGGA 
TCTCCAGGAA TCCCAGGAAT GGATGGAGAG CAGGGACTCA AAGGCTCAAA GGGAGACATG 
GGGGACCCAG GTATGACAGG TGAAAAAGGA GGAATTGGAC TTCCTGGATT ACCGGGAGCC 
AATGGAATGA AAGGAGAAAA AGGAGATTCT GGAATGCCGG GTCCACAGGG TCCTTCTATC 
ATAGGCCCAC CAGGCCCACC AGGTCCCCAT GGCCCACCTG GCCCCATGGG ACCTCATGGA 
CTTCCTGGAC CAAAGGGTGA ACCAGGGTTA AATGGTGTTA AAGGATTGAA GGGTGAACCA 
GGTCAAAAGG GTGACAGAGG ACCCCTTGGT CTTCCAGGAG CATCTGGCTT AGACGGAAAG 
CCAGGATCCC GGGGTACAGA TGGTCCTATG GGACCCCATG GCCCTGCAGG TCCCAAAGGA 
GAAAGAGGTG AAAAAGGAGC TATGGGAGAG CCTGGACCAA GAGGGCCCTA TGGGCTGCCT 
GGCTTCCCTG GTCCACGGGG TGAGAAGGGT GATCTAGGAG AAAAGGGAGA AAAGGTGACC 
TCTCCATCCC AGCATGTACC TTGTTTGATT CTTCTCCTGC TGTCTGCTTT GCTGTTCAGC 
TTGTGTGATT CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( NM_001256074.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243003.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11177
Clone ID Related Accession (Same CDS sequence)	NM_001256074.1 , NM_001256074.2
Accession Version	NM_001256074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559923200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004051.1, BC030649.1 and AC097473.3. On May 31, 2019 this sequence version replaced NM_001256074.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, and also differs in the 5' and 3' UTRs, compared to variant 1. The resulting isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGGGCCTGCC AGGCTTCCCC ACAGTTGCTG CCCTGCACAG TAATCAGATC 
CTCACCGTCA AGGGTGACCA AGGACAGGCA GGGCCTCCAG GACCCCCTGG CCCTCCAGGC 
CCAAGAGGGC CACCTGGGGA CACAGGGAAA GATGGCCCCC GTGGAATGCC AGGAGTACCC 
GGTGAACCAG GAAAGCCAGG AGAACAAGGC TTGATGGGTC CTCTAGGGCC TCCGGGACAA 
AAGGGTTCTA TTGGAGCACC TGGAATTCCA GGGATGAATG GGCAAAAGGG TGAGCCCGGG 
TTGCCTGGAG CAGTAGGACA GAATGGAATA CCAGGACCTA AGGGAGAACC TGGAGAACAA 
GGTGAAAAGG GAGACGCTGG AGAGAACGGC CCCAAGGGTG ACACAGGCGA AAAGGGTGAC 
CCTGGATCAT CTGCTGCAGG AATTAAGGGA GAACCTGGGG AATCTGGTCG TCCAGGGCAA 
AAGGGTGAAC CAGGGCTTCC TGGGCTTCCT GGACTTCCGG GGATAAAGGG AGAACCAGGT 
TTCATTGGTC CTCAAGGAGA ACCAGGCTTA CCAGGTTTAC CAGGAACAAA AGGTGAACGG 
GGGGAAGCAG GGCCTCCTGG AAGAGGTGAG CGAGGGGAAC CTGGAGCCCC CGGACCAAAG 
GGGGATCGTG GAGAAAAAGG GGACTCTGGA GCTCAGGGAC CAAGGGGTCC ACCTGGTCAA 
AAAGGGGATC AAGGAGCCAC TGAGATCATA GACTACAACG GCAACCTCCA CGAAGCCTTA 
CAGGGTCCCC CTGGACCTCC TGGACCTCAA GGACTACAAG GGCCAAAGGG AGAGCAGGGA 
TCTCCAGGAA TCCCAGGAAT GGATGGAGAG CAGGGACTCA AAGGCTCAAA GGGAGACATG 
GGGGACCCAG GTATGACAGG TGAAAAAGGA GGAATTGGAC TTCCTGGATT ACCGGGAGCC 
AATGGAATGA AAGGAGAAAA AGGAGATTCT GGAATGCCGG GTCCACAGGG TCCTTCTATC 
ATAGGCCCAC CAGGCCCACC AGGTCCCCAT GGCCCACCTG GCCCCATGGG ACCTCATGGA 
CTTCCTGGAC CAAAGGGTGA ACCAGGGTTA AATGGTGTTA AAGGATTGAA GGGTGAACCA 
GGTCAAAAGG GTGACAGAGG ACCCCTTGGT CTTCCAGGAG CATCTGGCTT AGACGGAAAG 
CCAGGATCCC GGGGTACAGA TGGTCCTATG GGACCCCATG GCCCTGCAGG TCCCAAAGGA 
GAAAGAGGTG AAAAAGGAGC TATGGGAGAG CCTGGACCAA GAGGGCCCTA TGGGCTGCCT 
GGCTTCCCTG GTCCACGGGG TGAGAAGGGT GATCTAGGAG AAAAGGGAGA AAAGGTGACC 
TCTCCATCCC AGCATGTACC TTGTTTGATT CTTCTCCTGC TGTCTGCTTT GCTGTTCAGC 
TTGTGTGATT CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243003.1
CDS	640..2577
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRGLPGFPTVAALHSNQILTVKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGFPGPRGEKGDLGEKGEKVTSPSQHVPCLILLLLSALLFSLCDSI

Target ORF information:

RefSeq Version	NM_001256074.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256074.2

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGGGCCTGCC AGGCTTCCCC ACAGTTGCTG CCCTGCACAG TAATCAGATC 
CTCACCGTCA AGGGTGACCA AGGACAGGCA GGGCCTCCAG GACCCCCTGG CCCTCCAGGC 
CCAAGAGGGC CACCTGGGGA CACAGGGAAA GATGGCCCCC GTGGAATGCC AGGAGTACCC 
GGTGAACCAG GAAAGCCAGG AGAACAAGGC TTGATGGGTC CTCTAGGGCC TCCGGGACAA 
AAGGGTTCTA TTGGAGCACC TGGAATTCCA GGGATGAATG GGCAAAAGGG TGAGCCCGGG 
TTGCCTGGAG CAGTAGGACA GAATGGAATA CCAGGACCTA AGGGAGAACC TGGAGAACAA 
GGTGAAAAGG GAGACGCTGG AGAGAACGGC CCCAAGGGTG ACACAGGCGA AAAGGGTGAC 
CCTGGATCAT CTGCTGCAGG AATTAAGGGA GAACCTGGGG AATCTGGTCG TCCAGGGCAA 
AAGGGTGAAC CAGGGCTTCC TGGGCTTCCT GGACTTCCGG GGATAAAGGG AGAACCAGGT 
TTCATTGGTC CTCAAGGAGA ACCAGGCTTA CCAGGTTTAC CAGGAACAAA AGGTGAACGG 
GGGGAAGCAG GGCCTCCTGG AAGAGGTGAG CGAGGGGAAC CTGGAGCCCC CGGACCAAAG 
GGGGATCGTG GAGAAAAAGG GGACTCTGGA GCTCAGGGAC CAAGGGGTCC ACCTGGTCAA 
AAAGGGGATC AAGGAGCCAC TGAGATCATA GACTACAACG GCAACCTCCA CGAAGCCTTA 
CAGGGTCCCC CTGGACCTCC TGGACCTCAA GGACTACAAG GGCCAAAGGG AGAGCAGGGA 
TCTCCAGGAA TCCCAGGAAT GGATGGAGAG CAGGGACTCA AAGGCTCAAA GGGAGACATG 
GGGGACCCAG GTATGACAGG TGAAAAAGGA GGAATTGGAC TTCCTGGATT ACCGGGAGCC 
AATGGAATGA AAGGAGAAAA AGGAGATTCT GGAATGCCGG GTCCACAGGG TCCTTCTATC 
ATAGGCCCAC CAGGCCCACC AGGTCCCCAT GGCCCACCTG GCCCCATGGG ACCTCATGGA 
CTTCCTGGAC CAAAGGGTGA ACCAGGGTTA AATGGTGTTA AAGGATTGAA GGGTGAACCA 
GGTCAAAAGG GTGACAGAGG ACCCCTTGGT CTTCCAGGAG CATCTGGCTT AGACGGAAAG 
CCAGGATCCC GGGGTACAGA TGGTCCTATG GGACCCCATG GCCCTGCAGG TCCCAAAGGA 
GAAAGAGGTG AAAAAGGAGC TATGGGAGAG CCTGGACCAA GAGGGCCCTA TGGGCTGCCT 
GGCTTCCCTG GTCCACGGGG TGAGAAGGGT GATCTAGGAG AAAAGGGAGA AAAGGTGACC 
TCTCCATCCC AGCATGTACC TTGTTTGATT CTTCTCCTGC TGTCTGCTTT GCTGTTCAGC 
TTGTGTGATT CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_011532335.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530637.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu73579
Clone ID Related Accession (Same CDS sequence)	XM_011532335.2 , XM_011532334.2 , XM_011532333.2
Accession Version	XM_011532335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTG AACCAGGGTT AAATGGTGTT 
AAAGGATTGA AGGGTGAACC AGGTCAAAAG GGTGACAGAG GACCCCTTGG TCTTCCAGGA 
GCATCTGGCT TAGACGGAAA GCCAGGATCC CGGGGTACAG ATGGTCCTAT GGGACCCCAT 
GGCCCTGCAG GTCCCAAAGG AGAAAGAGGT GAAAAAGGAG CTATGGGAGA GCCTGGACCA 
AGAGGGCCCT ATGGGCTGCC TGGGAAAGAT GGAGAGCCTG GTCTTGATGG CTTCCCTGGT 
CCACGGGGTG AGAAGGGTGA TCTAGGAGAA AAGGGAGAAA AGGGGGAAAA GGGAAAGAAA 
GGCAAAAAGG GGCCTAAAGG GGAGAAAGGA GAACAGGGTG CTCCTGGATT AGATGCACCC 
TGCCCGTTGG GGCCAGATGG CTTACCCATG CCTGGCTGTT GGCAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530637.1
CDS	320..2410
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGEKGKKGKKGPKGEKGEQGAPGLDAPCPLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_011532335.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532335.2

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTG AACCAGGGTT AAATGGTGTT 
AAAGGATTGA AGGGTGAACC AGGTCAAAAG GGTGACAGAG GACCCCTTGG TCTTCCAGGA 
GCATCTGGCT TAGACGGAAA GCCAGGATCC CGGGGTACAG ATGGTCCTAT GGGACCCCAT 
GGCCCTGCAG GTCCCAAAGG AGAAAGAGGT GAAAAAGGAG CTATGGGAGA GCCTGGACCA 
AGAGGGCCCT ATGGGCTGCC TGGGAAAGAT GGAGAGCCTG GTCTTGATGG CTTCCCTGGT 
CCACGGGGTG AGAAGGGTGA TCTAGGAGAA AAGGGAGAAA AGGGGGAAAA GGGAAAGAAA 
GGCAAAAAGG GGCCTAAAGG GGAGAAAGGA GAACAGGGTG CTCCTGGATT AGATGCACCC 
TGCCCGTTGG GGCCAGATGG CTTACCCATG CCTGGCTGTT GGCAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_011532334.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530636.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X2, mRNA.

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CloneID	OHu73579
Clone ID Related Accession (Same CDS sequence)	XM_011532335.2 , XM_011532334.2 , XM_011532333.2
Accession Version	XM_011532334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTG AACCAGGGTT AAATGGTGTT 
AAAGGATTGA AGGGTGAACC AGGTCAAAAG GGTGACAGAG GACCCCTTGG TCTTCCAGGA 
GCATCTGGCT TAGACGGAAA GCCAGGATCC CGGGGTACAG ATGGTCCTAT GGGACCCCAT 
GGCCCTGCAG GTCCCAAAGG AGAAAGAGGT GAAAAAGGAG CTATGGGAGA GCCTGGACCA 
AGAGGGCCCT ATGGGCTGCC TGGGAAAGAT GGAGAGCCTG GTCTTGATGG CTTCCCTGGT 
CCACGGGGTG AGAAGGGTGA TCTAGGAGAA AAGGGAGAAA AGGGGGAAAA GGGAAAGAAA 
GGCAAAAAGG GGCCTAAAGG GGAGAAAGGA GAACAGGGTG CTCCTGGATT AGATGCACCC 
TGCCCGTTGG GGCCAGATGG CTTACCCATG CCTGGCTGTT GGCAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530636.1
CDS	491..2581
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGEKGKKGKKGPKGEKGEQGAPGLDAPCPLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_011532334.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532334.2

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTG AACCAGGGTT AAATGGTGTT 
AAAGGATTGA AGGGTGAACC AGGTCAAAAG GGTGACAGAG GACCCCTTGG TCTTCCAGGA 
GCATCTGGCT TAGACGGAAA GCCAGGATCC CGGGGTACAG ATGGTCCTAT GGGACCCCAT 
GGCCCTGCAG GTCCCAAAGG AGAAAGAGGT GAAAAAGGAG CTATGGGAGA GCCTGGACCA 
AGAGGGCCCT ATGGGCTGCC TGGGAAAGAT GGAGAGCCTG GTCTTGATGG CTTCCCTGGT 
CCACGGGGTG AGAAGGGTGA TCTAGGAGAA AAGGGAGAAA AGGGGGAAAA GGGAAAGAAA 
GGCAAAAAGG GGCCTAAAGG GGAGAAAGGA GAACAGGGTG CTCCTGGATT AGATGCACCC 
TGCCCGTTGG GGCCAGATGG CTTACCCATG CCTGGCTGTT GGCAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_011532333.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530635.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X1, mRNA.

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CloneID	OHu73579
Clone ID Related Accession (Same CDS sequence)	XM_011532335.2 , XM_011532334.2 , XM_011532333.2
Accession Version	XM_011532333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTG AACCAGGGTT AAATGGTGTT 
AAAGGATTGA AGGGTGAACC AGGTCAAAAG GGTGACAGAG GACCCCTTGG TCTTCCAGGA 
GCATCTGGCT TAGACGGAAA GCCAGGATCC CGGGGTACAG ATGGTCCTAT GGGACCCCAT 
GGCCCTGCAG GTCCCAAAGG AGAAAGAGGT GAAAAAGGAG CTATGGGAGA GCCTGGACCA 
AGAGGGCCCT ATGGGCTGCC TGGGAAAGAT GGAGAGCCTG GTCTTGATGG CTTCCCTGGT 
CCACGGGGTG AGAAGGGTGA TCTAGGAGAA AAGGGAGAAA AGGGGGAAAA GGGAAAGAAA 
GGCAAAAAGG GGCCTAAAGG GGAGAAAGGA GAACAGGGTG CTCCTGGATT AGATGCACCC 
TGCCCGTTGG GGCCAGATGG CTTACCCATG CCTGGCTGTT GGCAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530635.1
CDS	625..2715
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGEKGKKGKKGPKGEKGEQGAPGLDAPCPLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_011532333.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532333.2

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTG AACCAGGGTT AAATGGTGTT 
AAAGGATTGA AGGGTGAACC AGGTCAAAAG GGTGACAGAG GACCCCTTGG TCTTCCAGGA 
GCATCTGGCT TAGACGGAAA GCCAGGATCC CGGGGTACAG ATGGTCCTAT GGGACCCCAT 
GGCCCTGCAG GTCCCAAAGG AGAAAGAGGT GAAAAAGGAG CTATGGGAGA GCCTGGACCA 
AGAGGGCCCT ATGGGCTGCC TGGGAAAGAT GGAGAGCCTG GTCTTGATGG CTTCCCTGGT 
CCACGGGGTG AGAAGGGTGA TCTAGGAGAA AAGGGAGAAA AGGGGGAAAA GGGAAAGAAA 
GGCAAAAAGG GGCCTAAAGG GGAGAAAGGA GAACAGGGTG CTCCTGGATT AGATGCACCC 
TGCCCGTTGG GGCCAGATGG CTTACCCATG CCTGGCTGTT GGCAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_011532338.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530640.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X4, mRNA.

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CloneID	OHu73582
Clone ID Related Accession (Same CDS sequence)	XM_011532338.2
Accession Version	XM_011532338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGCCCTCCTG GTCCAAAAGG CGATAAGGGA GAACAAGGTG ATCAGGGACC TAGGATGGTG 
TTTCCTAAAA TCAATCATGG GTTTCTCTCT GCTGATCAGC AGCTCATTAA ACGCCGCCTG 
ATTAAGGGTG ACCAAGGACA GGCAGGGCCT CCAGGACCCC CTGGCCCTCC AGGCCCAAGA 
GGGCCACCTG GGGACACAGG GAAAGATGGC CCCCGTGGAA TGCCAGGAGT ACCCGGTGAA 
CCAGGAAAGC CAGGAGAACA AGGCTTGATG GGTCCTCTAG GGCCTCCGGG ACAAAAGGGT 
TCTATTGGAG CACCTGGAAT TCCAGGGATG AATGGGCAAA AGGGTGAGCC CGGGTTGCCT 
GGAGCAGTAG GACAGAATGG AATACCAGGA CCTAAGGGAG AACCTGGAGA ACAAGGTGAA 
AAGGGAGACG CTGGAGAGAA CGGCCCCAAG GGTGACACAG GCGAAAAGGG TGACCCTGGA 
TCATCTGCTG CAGGAATTAA GGGAGAACCT GGGGAATCTG GTCGTCCAGG GCAAAAGGGT 
GAACCAGGGC TTCCTGGGCT TCCTGGACTT CCGGGGATAA AGGGAGAACC AGGTTTCATT 
GGTCCTCAAG GAGAACCAGG CTTACCAGGT TTACCAGGAA CAAAAGGTGA ACGGGGGGAA 
GCAGGGCCTC CTGGAAGAGG TGAGCGAGGG GAACCTGGAG CCCCCGGACC AAAGGGGAAA 
CAAGGTGAAT CAGGAACTAG AGGCCCAAAG GGGTCAAAGG GGGATCGTGG AGAAAAAGGG 
GACTCTGGAG CTCAGGGACC AAGGGGTCCA CCTGGTCAAA AAGGGGATCA AGGAGCCACT 
GAGATCATAG ACTACAACGG CAACCTCCAC GAAGCCTTAC AGAGGATTAC CACCTTAACT 
GTCACGGGTC CCCCTGGACC TCCTGGACCT CAAGGACTAC AAGGGCCAAA GGGAGAGCAG 
GGATCTCCAG GAATCCCAGG AATGGATGGA GAGCAGGGAC TCAAAGGCTC AAAGGGAGAC 
ATGGGGGACC CAGGTATGAC AGGTGAAAAA GGAGGAATTG GACTTCCTGG ATTACCGGGA 
GCCAATGGAA TGAAAGGAGA AAAAGGAGAT TCTGGAATGC CGGGTCCACA GGGTCCTTCT 
ATCATAGGCC CACCAGGCCC ACCAGGTCCC CATGGCCCAC CTGGCCCCAT GGGACCTCAT 
GGACTTCCTG GACCAAAGGG TGAACCAGGG TTAAATGGTG TTAAAGGATT GAAGGGTGAA 
CCAGGTCAAA AGGGTGACAG AGGACCCCTT GGTCTTCCAG GAGCATCTGG CTTAGACGGA 
AAGCCAGGAT CCCGGGGTAC AGATGGTCCT ATGGGACCCC ATGGCCCTGC AGGTCCCAAA 
GGAGAAAGAG GTGAAAAAGG AGCTATGGGA GAGCCTGGAC CAAGAGGGCC CTATGGGCTG 
CCTGGGAAAG ATGGAGAGCC TGGTCTTGAT GGCTTCCCTG GTCCACGGGG TGAGAAGGGT 
GATCTAGGAG AAAAGGGAGA AAAGGGGGAA AAGGGAAAGA AAGGCAAAAA GGGGCCTAAA 
GGGGAGAAAG GAGAACAGGG TGCTCCTGGA TTAGATGCAC CCTGCCCGTT GGGGCCAGAT 
GGCTTACCCA TGCCTGGCTG TTGGCAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530640.1
CDS	718..2790
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGEKGKKGKKGPKGEKGEQGAPGLDAPCPLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_011532338.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532338.2

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGCCCTCCTG GTCCAAAAGG CGATAAGGGA GAACAAGGTG ATCAGGGACC TAGGATGGTG 
TTTCCTAAAA TCAATCATGG GTTTCTCTCT GCTGATCAGC AGCTCATTAA ACGCCGCCTG 
ATTAAGGGTG ACCAAGGACA GGCAGGGCCT CCAGGACCCC CTGGCCCTCC AGGCCCAAGA 
GGGCCACCTG GGGACACAGG GAAAGATGGC CCCCGTGGAA TGCCAGGAGT ACCCGGTGAA 
CCAGGAAAGC CAGGAGAACA AGGCTTGATG GGTCCTCTAG GGCCTCCGGG ACAAAAGGGT 
TCTATTGGAG CACCTGGAAT TCCAGGGATG AATGGGCAAA AGGGTGAGCC CGGGTTGCCT 
GGAGCAGTAG GACAGAATGG AATACCAGGA CCTAAGGGAG AACCTGGAGA ACAAGGTGAA 
AAGGGAGACG CTGGAGAGAA CGGCCCCAAG GGTGACACAG GCGAAAAGGG TGACCCTGGA 
TCATCTGCTG CAGGAATTAA GGGAGAACCT GGGGAATCTG GTCGTCCAGG GCAAAAGGGT 
GAACCAGGGC TTCCTGGGCT TCCTGGACTT CCGGGGATAA AGGGAGAACC AGGTTTCATT 
GGTCCTCAAG GAGAACCAGG CTTACCAGGT TTACCAGGAA CAAAAGGTGA ACGGGGGGAA 
GCAGGGCCTC CTGGAAGAGG TGAGCGAGGG GAACCTGGAG CCCCCGGACC AAAGGGGAAA 
CAAGGTGAAT CAGGAACTAG AGGCCCAAAG GGGTCAAAGG GGGATCGTGG AGAAAAAGGG 
GACTCTGGAG CTCAGGGACC AAGGGGTCCA CCTGGTCAAA AAGGGGATCA AGGAGCCACT 
GAGATCATAG ACTACAACGG CAACCTCCAC GAAGCCTTAC AGAGGATTAC CACCTTAACT 
GTCACGGGTC CCCCTGGACC TCCTGGACCT CAAGGACTAC AAGGGCCAAA GGGAGAGCAG 
GGATCTCCAG GAATCCCAGG AATGGATGGA GAGCAGGGAC TCAAAGGCTC AAAGGGAGAC 
ATGGGGGACC CAGGTATGAC AGGTGAAAAA GGAGGAATTG GACTTCCTGG ATTACCGGGA 
GCCAATGGAA TGAAAGGAGA AAAAGGAGAT TCTGGAATGC CGGGTCCACA GGGTCCTTCT 
ATCATAGGCC CACCAGGCCC ACCAGGTCCC CATGGCCCAC CTGGCCCCAT GGGACCTCAT 
GGACTTCCTG GACCAAAGGG TGAACCAGGG TTAAATGGTG TTAAAGGATT GAAGGGTGAA 
CCAGGTCAAA AGGGTGACAG AGGACCCCTT GGTCTTCCAG GAGCATCTGG CTTAGACGGA 
AAGCCAGGAT CCCGGGGTAC AGATGGTCCT ATGGGACCCC ATGGCCCTGC AGGTCCCAAA 
GGAGAAAGAG GTGAAAAAGG AGCTATGGGA GAGCCTGGAC CAAGAGGGCC CTATGGGCTG 
CCTGGGAAAG ATGGAGAGCC TGGTCTTGAT GGCTTCCCTG GTCCACGGGG TGAGAAGGGT 
GATCTAGGAG AAAAGGGAGA AAAGGGGGAA AAGGGAAAGA AAGGCAAAAA GGGGCCTAAA 
GGGGAGAAAG GAGAACAGGG TGCTCCTGGA TTAGATGCAC CCTGCCCGTT GGGGCCAGAT 
GGCTTACCCA TGCCTGGCTG TTGGCAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_011532355.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530657.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X7, mRNA.

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CloneID	OHu73599
Clone ID Related Accession (Same CDS sequence)	XM_011532355.2 , XM_017008737.1
Accession Version	XM_011532355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCC CACTGATGAC AATGCCAGGC CCTCCAGGGA AACGAGGTAA GAGAGGCCGA 
AGAGGAGAAT CTGGTCCTCC TGGACAGCCT GGTCCTCAGG GCCCTCCTGG TCCAAAAGGC 
GATAAGGGAG AACAAGGTGA TCAGGGACCT AGGATGGTGT TTCCTAAAAT CAATCATGGG 
TTTCTCTCTG CTGATCAGCA GCTCATTAAA CGCCGCCTGA TTAAGGGTGA CCAAGGACAG 
GCAGGGCCTC CAGGACCCCC TGGCCCTCCA GGCCCAAGAG GGCCACCTGG GGACACAGGG 
AAAGATGGCC CCCGTGGAAT GCCAGGAGTA CCCGGTGAAC CAGGAAAGCC AGGAGAACAA 
GGCTTGATGG GTCCTCTAGG GCCTCCGGGA CAAAAGGGTT CTATTGGAGC ACCTGGAATT 
CCAGGGATGA ATGGGCAAAA GGGTGAGCCC GGGTTGCCTG GAGCAGTAGG ACAGAATGGA 
ATACCAGGAC CTAAGGGAGA ACCTGGAGAA CAAGGTGAAA AGGGAGACGC TGGAGAGAAC 
GGCCCCAAGG GTGACACAGG CGAAAAGGGT GACCCTGGAT CATCTGCTGC AGGAATTAAG 
GGAGAACCTG GGGAATCTGG TCGTCCAGGG CAAAAGGGTG AACCAGGGCT TCCTGGGCTT 
CCTGGACTTC CGGGGATAAA GGGAGAACCA GGTTTCATTG GTCCTCAAGG AGAACCAGGC 
TTACCAGGTT TACCAGGAAC AAAAGGTGAA CGGGGGGAAG CAGGGCCTCC TGGAAGAGGT 
GAGCGAGGGG AACCTGGAGC CCCCGGACCA AAGGGGAAAC AAGGTGAATC AGGAACTAGA 
GGCCCAAAGG GGTCAAAGGG GGATCGTGGA GAAAAAGGGG ACTCTGGAGC TCAGGGACCA 
AGGGGTCCAC CTGGTCAAAA AGGGGATCAA GGAGCCACTG AGATCATAGA CTACAACGGC 
AACCTCCACG AAGCCTTACA GAGGATTACC ACCTTAACTG TCACGGGTCC CCCTGGACCT 
CCTGGACCTC AAGGACTACA AGGGCCAAAG GGAGAGCAGG GATCTCCAGG AATCCCAGGA 
ATGGATGGAG AGCAGGGACT CAAAGGCTCA AAGGGAGACA TGGGGGACCC AGGTATGACA 
GGTGAAAAAG GAGGAATTGG ACTTCCTGGA TTACCGGGAG CCAATGGAAT GAAAGGAGAA 
AAAGGAGATT CTGGAATGCC GGGTCCACAG GGTCCTTCTA TCATAGGCCC ACCAGGCCCA 
CCAGGTCCCC ATGGCCCACC TGGCCCCATG GGACCTCATG GACTTCCTGG ACCAAAGGGT 
GAACCAGGGT TAAATGGTGT TAAAGGATTG AAGGGTGAAC CAGGTCAAAA GGGTGACAGA 
GGACCCCTTG GTCTTCCAGG AGCATCTGGC TTAGACGGAA AGCCAGGATC CCGGGGTACA 
GATGGTCCTA TGGGACCCCA TGGCCCTGCA GGTCCCAAAG GAGAAAGAGG TGAAAAAGGA 
GCTATGGGAG AGCCTGGACC AAGAGGGCCC TATGGGCTGC CTGGGAAAGA TGGAGAGCCT 
GGTCTTGATG GCTTCCCTGG TCCACGGGGT GAGAAGGGTG ATCTAGGAGA AAAGGGAGAA 
AAGGGGGAAA AGGGAAAGAA AGGCAAAAAG GGGCCTAAAG GGGAGAAAGG AGAACAGGGT 
GCTCCTGGAT TAGATGCACC CTGCCCGTTG GGGCCAGATG GCTTACCCAT GCCTGGCTGT 
TGGCAAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530657.1
CDS	4743..6494
Translation	MGCPLMTMPGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGEKGKKGKKGPKGEKGEQGAPGLDAPCPLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_011532355.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532355.2

ORF Insert Sequence:

ATGGGATGCC CACTGATGAC AATGCCAGGC CCTCCAGGGA AACGAGGTAA GAGAGGCCGA 
AGAGGAGAAT CTGGTCCTCC TGGACAGCCT GGTCCTCAGG GCCCTCCTGG TCCAAAAGGC 
GATAAGGGAG AACAAGGTGA TCAGGGACCT AGGATGGTGT TTCCTAAAAT CAATCATGGG 
TTTCTCTCTG CTGATCAGCA GCTCATTAAA CGCCGCCTGA TTAAGGGTGA CCAAGGACAG 
GCAGGGCCTC CAGGACCCCC TGGCCCTCCA GGCCCAAGAG GGCCACCTGG GGACACAGGG 
AAAGATGGCC CCCGTGGAAT GCCAGGAGTA CCCGGTGAAC CAGGAAAGCC AGGAGAACAA 
GGCTTGATGG GTCCTCTAGG GCCTCCGGGA CAAAAGGGTT CTATTGGAGC ACCTGGAATT 
CCAGGGATGA ATGGGCAAAA GGGTGAGCCC GGGTTGCCTG GAGCAGTAGG ACAGAATGGA 
ATACCAGGAC CTAAGGGAGA ACCTGGAGAA CAAGGTGAAA AGGGAGACGC TGGAGAGAAC 
GGCCCCAAGG GTGACACAGG CGAAAAGGGT GACCCTGGAT CATCTGCTGC AGGAATTAAG 
GGAGAACCTG GGGAATCTGG TCGTCCAGGG CAAAAGGGTG AACCAGGGCT TCCTGGGCTT 
CCTGGACTTC CGGGGATAAA GGGAGAACCA GGTTTCATTG GTCCTCAAGG AGAACCAGGC 
TTACCAGGTT TACCAGGAAC AAAAGGTGAA CGGGGGGAAG CAGGGCCTCC TGGAAGAGGT 
GAGCGAGGGG AACCTGGAGC CCCCGGACCA AAGGGGAAAC AAGGTGAATC AGGAACTAGA 
GGCCCAAAGG GGTCAAAGGG GGATCGTGGA GAAAAAGGGG ACTCTGGAGC TCAGGGACCA 
AGGGGTCCAC CTGGTCAAAA AGGGGATCAA GGAGCCACTG AGATCATAGA CTACAACGGC 
AACCTCCACG AAGCCTTACA GAGGATTACC ACCTTAACTG TCACGGGTCC CCCTGGACCT 
CCTGGACCTC AAGGACTACA AGGGCCAAAG GGAGAGCAGG GATCTCCAGG AATCCCAGGA 
ATGGATGGAG AGCAGGGACT CAAAGGCTCA AAGGGAGACA TGGGGGACCC AGGTATGACA 
GGTGAAAAAG GAGGAATTGG ACTTCCTGGA TTACCGGGAG CCAATGGAAT GAAAGGAGAA 
AAAGGAGATT CTGGAATGCC GGGTCCACAG GGTCCTTCTA TCATAGGCCC ACCAGGCCCA 
CCAGGTCCCC ATGGCCCACC TGGCCCCATG GGACCTCATG GACTTCCTGG ACCAAAGGGT 
GAACCAGGGT TAAATGGTGT TAAAGGATTG AAGGGTGAAC CAGGTCAAAA GGGTGACAGA 
GGACCCCTTG GTCTTCCAGG AGCATCTGGC TTAGACGGAA AGCCAGGATC CCGGGGTACA 
GATGGTCCTA TGGGACCCCA TGGCCCTGCA GGTCCCAAAG GAGAAAGAGG TGAAAAAGGA 
GCTATGGGAG AGCCTGGACC AAGAGGGCCC TATGGGCTGC CTGGGAAAGA TGGAGAGCCT 
GGTCTTGATG GCTTCCCTGG TCCACGGGGT GAGAAGGGTG ATCTAGGAGA AAAGGGAGAA 
AAGGGGGAAA AGGGAAAGAA AGGCAAAAAG GGGCCTAAAG GGGAGAAAGG AGAACAGGGT 
GCTCCTGGAT TAGATGCACC CTGCCCGTTG GGGCCAGATG GCTTACCCAT GCCTGGCTGT 
TGGCAAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_017008737.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864226.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu73599
Clone ID Related Accession (Same CDS sequence)	XM_011532355.2 , XM_017008737.1
Accession Version	XM_017008737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCC CACTGATGAC AATGCCAGGC CCTCCAGGGA AACGAGGTAA GAGAGGCCGA 
AGAGGAGAAT CTGGTCCTCC TGGACAGCCT GGTCCTCAGG GCCCTCCTGG TCCAAAAGGC 
GATAAGGGAG AACAAGGTGA TCAGGGACCT AGGATGGTGT TTCCTAAAAT CAATCATGGG 
TTTCTCTCTG CTGATCAGCA GCTCATTAAA CGCCGCCTGA TTAAGGGTGA CCAAGGACAG 
GCAGGGCCTC CAGGACCCCC TGGCCCTCCA GGCCCAAGAG GGCCACCTGG GGACACAGGG 
AAAGATGGCC CCCGTGGAAT GCCAGGAGTA CCCGGTGAAC CAGGAAAGCC AGGAGAACAA 
GGCTTGATGG GTCCTCTAGG GCCTCCGGGA CAAAAGGGTT CTATTGGAGC ACCTGGAATT 
CCAGGGATGA ATGGGCAAAA GGGTGAGCCC GGGTTGCCTG GAGCAGTAGG ACAGAATGGA 
ATACCAGGAC CTAAGGGAGA ACCTGGAGAA CAAGGTGAAA AGGGAGACGC TGGAGAGAAC 
GGCCCCAAGG GTGACACAGG CGAAAAGGGT GACCCTGGAT CATCTGCTGC AGGAATTAAG 
GGAGAACCTG GGGAATCTGG TCGTCCAGGG CAAAAGGGTG AACCAGGGCT TCCTGGGCTT 
CCTGGACTTC CGGGGATAAA GGGAGAACCA GGTTTCATTG GTCCTCAAGG AGAACCAGGC 
TTACCAGGTT TACCAGGAAC AAAAGGTGAA CGGGGGGAAG CAGGGCCTCC TGGAAGAGGT 
GAGCGAGGGG AACCTGGAGC CCCCGGACCA AAGGGGAAAC AAGGTGAATC AGGAACTAGA 
GGCCCAAAGG GGTCAAAGGG GGATCGTGGA GAAAAAGGGG ACTCTGGAGC TCAGGGACCA 
AGGGGTCCAC CTGGTCAAAA AGGGGATCAA GGAGCCACTG AGATCATAGA CTACAACGGC 
AACCTCCACG AAGCCTTACA GAGGATTACC ACCTTAACTG TCACGGGTCC CCCTGGACCT 
CCTGGACCTC AAGGACTACA AGGGCCAAAG GGAGAGCAGG GATCTCCAGG AATCCCAGGA 
ATGGATGGAG AGCAGGGACT CAAAGGCTCA AAGGGAGACA TGGGGGACCC AGGTATGACA 
GGTGAAAAAG GAGGAATTGG ACTTCCTGGA TTACCGGGAG CCAATGGAAT GAAAGGAGAA 
AAAGGAGATT CTGGAATGCC GGGTCCACAG GGTCCTTCTA TCATAGGCCC ACCAGGCCCA 
CCAGGTCCCC ATGGCCCACC TGGCCCCATG GGACCTCATG GACTTCCTGG ACCAAAGGGT 
GAACCAGGGT TAAATGGTGT TAAAGGATTG AAGGGTGAAC CAGGTCAAAA GGGTGACAGA 
GGACCCCTTG GTCTTCCAGG AGCATCTGGC TTAGACGGAA AGCCAGGATC CCGGGGTACA 
GATGGTCCTA TGGGACCCCA TGGCCCTGCA GGTCCCAAAG GAGAAAGAGG TGAAAAAGGA 
GCTATGGGAG AGCCTGGACC AAGAGGGCCC TATGGGCTGC CTGGGAAAGA TGGAGAGCCT 
GGTCTTGATG GCTTCCCTGG TCCACGGGGT GAGAAGGGTG ATCTAGGAGA AAAGGGAGAA 
AAGGGGGAAA AGGGAAAGAA AGGCAAAAAG GGGCCTAAAG GGGAGAAAGG AGAACAGGGT 
GCTCCTGGAT TAGATGCACC CTGCCCGTTG GGGCCAGATG GCTTACCCAT GCCTGGCTGT 
TGGCAAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864226.1
CDS	3420..5171
Translation	MGCPLMTMPGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGEKGKKGKKGPKGEKGEQGAPGLDAPCPLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_017008737.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008737.1

ORF Insert Sequence:

ATGGGATGCC CACTGATGAC AATGCCAGGC CCTCCAGGGA AACGAGGTAA GAGAGGCCGA 
AGAGGAGAAT CTGGTCCTCC TGGACAGCCT GGTCCTCAGG GCCCTCCTGG TCCAAAAGGC 
GATAAGGGAG AACAAGGTGA TCAGGGACCT AGGATGGTGT TTCCTAAAAT CAATCATGGG 
TTTCTCTCTG CTGATCAGCA GCTCATTAAA CGCCGCCTGA TTAAGGGTGA CCAAGGACAG 
GCAGGGCCTC CAGGACCCCC TGGCCCTCCA GGCCCAAGAG GGCCACCTGG GGACACAGGG 
AAAGATGGCC CCCGTGGAAT GCCAGGAGTA CCCGGTGAAC CAGGAAAGCC AGGAGAACAA 
GGCTTGATGG GTCCTCTAGG GCCTCCGGGA CAAAAGGGTT CTATTGGAGC ACCTGGAATT 
CCAGGGATGA ATGGGCAAAA GGGTGAGCCC GGGTTGCCTG GAGCAGTAGG ACAGAATGGA 
ATACCAGGAC CTAAGGGAGA ACCTGGAGAA CAAGGTGAAA AGGGAGACGC TGGAGAGAAC 
GGCCCCAAGG GTGACACAGG CGAAAAGGGT GACCCTGGAT CATCTGCTGC AGGAATTAAG 
GGAGAACCTG GGGAATCTGG TCGTCCAGGG CAAAAGGGTG AACCAGGGCT TCCTGGGCTT 
CCTGGACTTC CGGGGATAAA GGGAGAACCA GGTTTCATTG GTCCTCAAGG AGAACCAGGC 
TTACCAGGTT TACCAGGAAC AAAAGGTGAA CGGGGGGAAG CAGGGCCTCC TGGAAGAGGT 
GAGCGAGGGG AACCTGGAGC CCCCGGACCA AAGGGGAAAC AAGGTGAATC AGGAACTAGA 
GGCCCAAAGG GGTCAAAGGG GGATCGTGGA GAAAAAGGGG ACTCTGGAGC TCAGGGACCA 
AGGGGTCCAC CTGGTCAAAA AGGGGATCAA GGAGCCACTG AGATCATAGA CTACAACGGC 
AACCTCCACG AAGCCTTACA GAGGATTACC ACCTTAACTG TCACGGGTCC CCCTGGACCT 
CCTGGACCTC AAGGACTACA AGGGCCAAAG GGAGAGCAGG GATCTCCAGG AATCCCAGGA 
ATGGATGGAG AGCAGGGACT CAAAGGCTCA AAGGGAGACA TGGGGGACCC AGGTATGACA 
GGTGAAAAAG GAGGAATTGG ACTTCCTGGA TTACCGGGAG CCAATGGAAT GAAAGGAGAA 
AAAGGAGATT CTGGAATGCC GGGTCCACAG GGTCCTTCTA TCATAGGCCC ACCAGGCCCA 
CCAGGTCCCC ATGGCCCACC TGGCCCCATG GGACCTCATG GACTTCCTGG ACCAAAGGGT 
GAACCAGGGT TAAATGGTGT TAAAGGATTG AAGGGTGAAC CAGGTCAAAA GGGTGACAGA 
GGACCCCTTG GTCTTCCAGG AGCATCTGGC TTAGACGGAA AGCCAGGATC CCGGGGTACA 
GATGGTCCTA TGGGACCCCA TGGCCCTGCA GGTCCCAAAG GAGAAAGAGG TGAAAAAGGA 
GCTATGGGAG AGCCTGGACC AAGAGGGCCC TATGGGCTGC CTGGGAAAGA TGGAGAGCCT 
GGTCTTGATG GCTTCCCTGG TCCACGGGGT GAGAAGGGTG ATCTAGGAGA AAAGGGAGAA 
AAGGGGGAAA AGGGAAAGAA AGGCAAAAAG GGGCCTAAAG GGGAGAAAGG AGAACAGGGT 
GCTCCTGGAT TAGATGCACC CTGCCCGTTG GGGCCAGATG GCTTACCCAT GCCTGGCTGT 
TGGCAAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_011532356.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530658.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X9, mRNA.

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CloneID	OHu73600
Clone ID Related Accession (Same CDS sequence)	XM_011532356.2
Accession Version	XM_011532356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCC CACTGATGAC AATGCCAGGC CCTCCAGGGA AACGAGGTAA GAGAGGCCGA 
AGAGGAGAAT CTGGTCCTCC TGGCCCTCCT GGTCCAAAAG GCGATAAGGG AGAACAAGGT 
GATCAGGGAC CTAGGATGGT GTTTCCTAAA ATCAATCATG GGTTTCTCTC TGCTGATCAG 
CAGCTCATTA AACGCCGCCT GATTAAGGGT GACCAAGGAC AGGCAGGGCC TCCAGGACCC 
CCTGGCCCTC CAGGCCCAAG AGGGCCACCT GGGGACACAG GGAAAGATGG CCCCCGTGGA 
ATGCCAGGAG TACCCGGTGA ACCAGGAAAG CCAGGAGAAC AAGGCTTGAT GGGTCCTCTA 
GGGCCTCCGG GACAAAAGGG TTCTATTGGA GCACCTGGAA TTCCAGGGAT GAATGGGCAA 
AAGGGTGAGC CCGGGTTGCC TGGAGCAGTA GGACAGAATG GAATACCAGG ACCTAAGGGA 
GAACCTGGAG AACAAGGTGA AAAGGGAGAC GCTGGAGAGA ACGGCCCCAA GGGTGACACA 
GGCGAAAAGG GTGACCCTGG ATCATCTGCT GCAGGAATTA AGGGAGAACC TGGGGAATCT 
GGTCGTCCAG GGCAAAAGGG TGAACCAGGG CTTCCTGGGC TTCCTGGACT TCCGGGGATA 
AAGGGAGAAC CAGGTTTCAT TGGTCCTCAA GGAGAACCAG GCTTACCAGG TTTACCAGGA 
ACAAAAGGTG AACGGGGGGA AGCAGGGCCT CCTGGAAGAG GTGAGCGAGG GGAACCTGGA 
GCCCCCGGAC CAAAGGGGAA ACAAGGTGAA TCAGGAACTA GAGGCCCAAA GGGGTCAAAG 
GGGGATCGTG GAGAAAAAGG GGACTCTGGA GCTCAGGGAC CAAGGGGTCC ACCTGGTCAA 
AAAGGGGATC AAGGAGCCAC TGAGATCATA GACTACAACG GCAACCTCCA CGAAGCCTTA 
CAGAGGATTA CCACCTTAAC TGTCACGGGT CCCCCTGGAC CTCCTGGACC TCAAGGACTA 
CAAGGGCCAA AGGGAGAGCA GGGATCTCCA GGAATCCCAG GAATGGATGG AGAGCAGGGA 
CTCAAAGGCT CAAAGGGAGA CATGGGGGAC CCAGGTATGA CAGGTGAAAA AGGAGGAATT 
GGACTTCCTG GATTACCGGG AGCCAATGGA ATGAAAGGAG AAAAAGGAGA TTCTGGAATG 
CCGGGTCCAC AGGGTCCTTC TATCATAGGC CCACCAGGCC CACCAGGTCC CCATGGCCCA 
CCTGGCCCCA TGGGACCTCA TGGACTTCCT GGACCAAAGG GTGAACCAGG GTTAAATGGT 
GTTAAAGGAT TGAAGGGTGA ACCAGGTCAA AAGGGTGACA GAGGACCCCT TGGTCTTCCA 
GGAGCATCTG GCTTAGACGG AAAGCCAGGA TCCCGGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGGGGA AAAGGGAAAG 
AAAGGCAAAA AGGGGCCTAA AGGGGAGAAA GGAGAACAGG GTGCTCCTGG ATTAGATGCA 
CCCTGCCCGT TGGGGCCAGA TGGCTTACCC ATGCCTGGCT GTTGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530658.1
CDS	4743..6476
Translation	MGCPLMTMPGPPGKRGKRGRRGESGPPGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGEKGKKGKKGPKGEKGEQGAPGLDAPCPLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_011532356.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532356.2

ORF Insert Sequence:

ATGGGATGCC CACTGATGAC AATGCCAGGC CCTCCAGGGA AACGAGGTAA GAGAGGCCGA 
AGAGGAGAAT CTGGTCCTCC TGGCCCTCCT GGTCCAAAAG GCGATAAGGG AGAACAAGGT 
GATCAGGGAC CTAGGATGGT GTTTCCTAAA ATCAATCATG GGTTTCTCTC TGCTGATCAG 
CAGCTCATTA AACGCCGCCT GATTAAGGGT GACCAAGGAC AGGCAGGGCC TCCAGGACCC 
CCTGGCCCTC CAGGCCCAAG AGGGCCACCT GGGGACACAG GGAAAGATGG CCCCCGTGGA 
ATGCCAGGAG TACCCGGTGA ACCAGGAAAG CCAGGAGAAC AAGGCTTGAT GGGTCCTCTA 
GGGCCTCCGG GACAAAAGGG TTCTATTGGA GCACCTGGAA TTCCAGGGAT GAATGGGCAA 
AAGGGTGAGC CCGGGTTGCC TGGAGCAGTA GGACAGAATG GAATACCAGG ACCTAAGGGA 
GAACCTGGAG AACAAGGTGA AAAGGGAGAC GCTGGAGAGA ACGGCCCCAA GGGTGACACA 
GGCGAAAAGG GTGACCCTGG ATCATCTGCT GCAGGAATTA AGGGAGAACC TGGGGAATCT 
GGTCGTCCAG GGCAAAAGGG TGAACCAGGG CTTCCTGGGC TTCCTGGACT TCCGGGGATA 
AAGGGAGAAC CAGGTTTCAT TGGTCCTCAA GGAGAACCAG GCTTACCAGG TTTACCAGGA 
ACAAAAGGTG AACGGGGGGA AGCAGGGCCT CCTGGAAGAG GTGAGCGAGG GGAACCTGGA 
GCCCCCGGAC CAAAGGGGAA ACAAGGTGAA TCAGGAACTA GAGGCCCAAA GGGGTCAAAG 
GGGGATCGTG GAGAAAAAGG GGACTCTGGA GCTCAGGGAC CAAGGGGTCC ACCTGGTCAA 
AAAGGGGATC AAGGAGCCAC TGAGATCATA GACTACAACG GCAACCTCCA CGAAGCCTTA 
CAGAGGATTA CCACCTTAAC TGTCACGGGT CCCCCTGGAC CTCCTGGACC TCAAGGACTA 
CAAGGGCCAA AGGGAGAGCA GGGATCTCCA GGAATCCCAG GAATGGATGG AGAGCAGGGA 
CTCAAAGGCT CAAAGGGAGA CATGGGGGAC CCAGGTATGA CAGGTGAAAA AGGAGGAATT 
GGACTTCCTG GATTACCGGG AGCCAATGGA ATGAAAGGAG AAAAAGGAGA TTCTGGAATG 
CCGGGTCCAC AGGGTCCTTC TATCATAGGC CCACCAGGCC CACCAGGTCC CCATGGCCCA 
CCTGGCCCCA TGGGACCTCA TGGACTTCCT GGACCAAAGG GTGAACCAGG GTTAAATGGT 
GTTAAAGGAT TGAAGGGTGA ACCAGGTCAA AAGGGTGACA GAGGACCCCT TGGTCTTCCA 
GGAGCATCTG GCTTAGACGG AAAGCCAGGA TCCCGGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGGGGA AAAGGGAAAG 
AAAGGCAAAA AGGGGCCTAA AGGGGAGAAA GGAGAACAGG GTGCTCCTGG ATTAGATGCA 
CCCTGCCCGT TGGGGCCAGA TGGCTTACCC ATGCCTGGCT GTTGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_011532358.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530660.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu73602
Clone ID Related Accession (Same CDS sequence)	XM_011532358.2
Accession Version	XM_011532358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAA TGGTGTTTCC TAAAATCAAT CATGGGTTTC TCTCTGCTGA TCAGCAGCTC 
ATTAAACGCC GCCTGATTAA GGGTGACCAA GGACAGGCAG GGCCTCCAGG ACCCCCTGGC 
CCTCCAGGCC CAAGAGGGCC ACCTGGGGAC ACAGGGAAAG ATGGCCCCCG TGGAATGCCA 
GGAGTACCCG GTGAACCAGG AAAGCCAGGA GAACAAGGCT TGATGGGTCC TCTAGGGCCT 
CCGGGACAAA AGGGTTCTAT TGGAGCACCT GGAATTCCAG GGATGAATGG GCAAAAGGGT 
GAGCCCGGGT TGCCTGGAGC AGTAGGACAG AATGGAATAC CAGGACCTAA GGGAGAACCT 
GGAGAACAAG GTGAAAAGGG AGACGCTGGA GAGAACGGCC CCAAGGGTGA CACAGGCGAA 
AAGGGTGACC CTGGATCATC TGCTGCAGGA ATTAAGGGAG AACCTGGGGA ATCTGGTCGT 
CCAGGGCAAA AGGGTGAACC AGGGCTTCCT GGGCTTCCTG GACTTCCGGG GATAAAGGGA 
GAACCAGGTT TCATTGGTCC TCAAGGAGAA CCAGGCTTAC CAGGTTTACC AGGAACAAAA 
GGTGAACGGG GGGAAGCAGG GCCTCCTGGA AGAGGTGAGC GAGGGGAACC TGGAGCCCCC 
GGACCAAAGG GGAAACAAGG TGAATCAGGA ACTAGAGGCC CAAAGGGGTC AAAGGGGGAT 
CGTGGAGAAA AAGGGGACTC TGGAGCTCAG GGACCAAGGG GTCCACCTGG TCAAAAAGGG 
GATCAAGGAG CCACTGAGAT CATAGACTAC AACGGCAACC TCCACGAAGC CTTACAGAGG 
ATTACCACCT TAACTGTCAC GGGTCCCCCT GGACCTCCTG GACCTCAAGG ACTACAAGGG 
CCAAAGGGAG AGCAGGGATC TCCAGGAATC CCAGGAATGG ATGGAGAGCA GGGACTCAAA 
GGCTCAAAGG GAGACATGGG GGACCCAGGT ATGACAGGTG AAAAAGGAGG AATTGGACTT 
CCTGGATTAC CGGGAGCCAA TGGAATGAAA GGAGAAAAAG GAGATTCTGG AATGCCGGGT 
CCACAGGGTC CTTCTATCAT AGGCCCACCA GGCCCACCAG GTCCCCATGG CCCACCTGGC 
CCCATGGGAC CTCATGGACT TCCTGGACCA AAGGGTGAAC CAGGGTTAAA TGGTGTTAAA 
GGATTGAAGG GTGAACCAGG TCAAAAGGGT GACAGAGGAC CCCTTGGTCT TCCAGGAGCA 
TCTGGCTTAG ACGGAAAGCC AGGATCCCGG GGTACAGATG GTCCTATGGG ACCCCATGGC 
CCTGCAGGTC CCAAAGGAGA AAGAGGTGAA AAAGGAGCTA TGGGAGAGCC TGGACCAAGA 
GGGCCCTATG GGCTGCCTGG GAAAGATGGA GAGCCTGGTC TTGATGGCTT CCCTGGTCCA 
CGGGGTGAGA AGGGTGATCT AGGAGAAAAG GGAGAAAAGG GGGAAAAGGG AAAGAAAGGC 
AAAAAGGGGC CTAAAGGGGA GAAAGGAGAA CAGGGTGCTC CTGGATTAGA TGCACCCTGC 
CCGTTGGGGC CAGATGGCTT ACCCATGCCT GGCTGTTGGC AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530660.1
CDS	279..1886
Translation	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

Target ORF information:

RefSeq Version	XM_011532358.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532358.2

ORF Insert Sequence:

ATGGAAACAA TGGTGTTTCC TAAAATCAAT CATGGGTTTC TCTCTGCTGA TCAGCAGCTC 
ATTAAACGCC GCCTGATTAA GGGTGACCAA GGACAGGCAG GGCCTCCAGG ACCCCCTGGC 
CCTCCAGGCC CAAGAGGGCC ACCTGGGGAC ACAGGGAAAG ATGGCCCCCG TGGAATGCCA 
GGAGTACCCG GTGAACCAGG AAAGCCAGGA GAACAAGGCT TGATGGGTCC TCTAGGGCCT 
CCGGGACAAA AGGGTTCTAT TGGAGCACCT GGAATTCCAG GGATGAATGG GCAAAAGGGT 
GAGCCCGGGT TGCCTGGAGC AGTAGGACAG AATGGAATAC CAGGACCTAA GGGAGAACCT 
GGAGAACAAG GTGAAAAGGG AGACGCTGGA GAGAACGGCC CCAAGGGTGA CACAGGCGAA 
AAGGGTGACC CTGGATCATC TGCTGCAGGA ATTAAGGGAG AACCTGGGGA ATCTGGTCGT 
CCAGGGCAAA AGGGTGAACC AGGGCTTCCT GGGCTTCCTG GACTTCCGGG GATAAAGGGA 
GAACCAGGTT TCATTGGTCC TCAAGGAGAA CCAGGCTTAC CAGGTTTACC AGGAACAAAA 
GGTGAACGGG GGGAAGCAGG GCCTCCTGGA AGAGGTGAGC GAGGGGAACC TGGAGCCCCC 
GGACCAAAGG GGAAACAAGG TGAATCAGGA ACTAGAGGCC CAAAGGGGTC AAAGGGGGAT 
CGTGGAGAAA AAGGGGACTC TGGAGCTCAG GGACCAAGGG GTCCACCTGG TCAAAAAGGG 
GATCAAGGAG CCACTGAGAT CATAGACTAC AACGGCAACC TCCACGAAGC CTTACAGAGG 
ATTACCACCT TAACTGTCAC GGGTCCCCCT GGACCTCCTG GACCTCAAGG ACTACAAGGG 
CCAAAGGGAG AGCAGGGATC TCCAGGAATC CCAGGAATGG ATGGAGAGCA GGGACTCAAA 
GGCTCAAAGG GAGACATGGG GGACCCAGGT ATGACAGGTG AAAAAGGAGG AATTGGACTT 
CCTGGATTAC CGGGAGCCAA TGGAATGAAA GGAGAAAAAG GAGATTCTGG AATGCCGGGT 
CCACAGGGTC CTTCTATCAT AGGCCCACCA GGCCCACCAG GTCCCCATGG CCCACCTGGC 
CCCATGGGAC CTCATGGACT TCCTGGACCA AAGGGTGAAC CAGGGTTAAA TGGTGTTAAA 
GGATTGAAGG GTGAACCAGG TCAAAAGGGT GACAGAGGAC CCCTTGGTCT TCCAGGAGCA 
TCTGGCTTAG ACGGAAAGCC AGGATCCCGG GGTACAGATG GTCCTATGGG ACCCCATGGC 
CCTGCAGGTC CCAAAGGAGA AAGAGGTGAA AAAGGAGCTA TGGGAGAGCC TGGACCAAGA 
GGGCCCTATG GGCTGCCTGG GAAAGATGGA GAGCCTGGTC TTGATGGCTT CCCTGGTCCA 
CGGGGTGAGA AGGGTGATCT AGGAGAAAAG GGAGAAAAGG GGGAAAAGGG AAAGAAAGGC 
AAAAAGGGGC CTAAAGGGGA GAAAGGAGAA CAGGGTGCTC CTGGATTAGA TGCACCCTGC 
CCGTTGGGGC CAGATGGCTT ACCCATGCCT GGCTGTTGGC AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_017008735.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864224.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X5, mRNA.

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CloneID	OHu95483
Clone ID Related Accession (Same CDS sequence)	XM_017008735.1
Accession Version	XM_017008735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGCCCTCCTG GTCCAAAAGG CGATAAGGGA GAACAAGGTG ATCAGGGACC TAGGATGGTG 
TTTCCTAAAA TCAATCATGG GTTTCTCTCT GCTGATCAGC AGCTCATTAA ACGCCGCCTG 
ATTAAGGGTG ACCAAGGACA GGCAGGGCCT CCAGGACCCC CTGGCCCTCC AGGCCCAAGA 
GGGCCACCTG GGGACACAGG GAAAGATGGC CCCCGTGGAA TGCCAGGAGT ACCCGGTGAA 
CCAGGAAAGC CAGGAGAACA AGGCTTGATG GGTCCTCTAG GGCCTCCGGG ACAAAAGGGT 
TCTATTGGAG CACCTGGAAT TCCAGGGATG AATGGGCAAA AGGGTGAGCC CGGGTTGCCT 
GGAGCAGTAG GACAGAATGG AATACCAGGA CCTAAGGGAG AACCTGGAGA ACAAGGTGAA 
AAGGGAGACG CTGGAGAGAA CGGCCCCAAG GGTGACACAG GCGAAAAGGG TGACCCTGGA 
TCATCTGCTG CAGGAATTAA GGGAGAACCT GGGGAATCTG GTCGTCCAGG GCAAAAGGGT 
GAACCAGGGC TTCCTGGGCT TCCTGGACTT CCGGGGATAA AGGGAGAACC AGGTTTCATT 
GGTCCTCAAG GAGAACCAGG CTTACCAGGT TTACCAGGAA CAAAAGGTGA ACGGGGGGAA 
GCAGGGCCTC CTGGAAGAGG TGAGCGAGGG GAACCTGGAG CCCCCGGACC AAAGGGGAAA 
CAAGGTGAAT CAGGAACTAG AGGCCCAAAG GGGTCAAAGG GGGATCGTGG AGAAAAAGGG 
GACTCTGGAG CTCAGGGACC AAGGGGTCCA CCTGGTCAAA AAGGGGATCA AGGAGCCACT 
GAGATCATAG ACTACAACGG CAACCTCCAC GAAGCCTTAC AGAGGATTAC CACCTTAACT 
GTCACGGGTC CCCCTGGACC TCCTGGACCT CAAGGACTAC AAGGGCCAAA GGGAGAGCAG 
GGATCTCCAG GAATCCCAGG AATGGATGGA GAGCAGGGAC TCAAAGGCTC AAAGGGAGAC 
ATGGGGGACC CAGGTATGAC AGGTGAAAAA GGAGGAATTG GACTTCCTGG ATTACCGGGA 
GCCAATGGAA TGAAAGGAGA AAAAGGAGAT TCTGGAATGC CGGGTCCACA GGGTCCTTCT 
ATCATAGGCC CACCAGGCCC ACCAGGTCCC CATGGCCCAC CTGGCCCCAT GGGACCTCAT 
GGACTTCCTG GACCAAAGGG TGAACCAGGG TTAAATGGTG TTAAAGGATT GAAGGGTGAA 
CCAGGTCAAA AGGGTGACAG AGGACCCCTT GGTCTTCCAG GAGCATCTGG CTTAGACGGA 
AAGCCAGGAT CCCGGGGTAC AGATGGTCCT ATGGGACCCC ATGGCCCTGC AGGTCCCAAA 
GGAGAAAGAG GTGAAAAAGG AGCTATGGGA GAGCCTGGAC CAAGAGGGCC CTATGGGCTG 
CCTGGCTTCC CTGGTCCACG GGGTGAGAAG GGTGATCTAG GAGAAAAGGG AGAAAAGGGA 
TTCCGTGGCG TTAAGGGGGA AAAAGGGGAG CCAGGCCAGC CTGGCCTGGA TGGGCTGGAT 
GCCCCTTGCC AATTGGGGCC AGATGGCTTA CCCATGCCTG GCTGTTGGCA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864224.1
CDS	321..2357
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGEPGLNGVKGLKGEPGQKGDRGPLGLPGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGFPGPRGEKGDLGEKGEKGFRGVKGEKGEPGQPGLDGLDAPCQLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_017008735.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008735.1

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGCCCTCCTG GTCCAAAAGG CGATAAGGGA GAACAAGGTG ATCAGGGACC TAGGATGGTG 
TTTCCTAAAA TCAATCATGG GTTTCTCTCT GCTGATCAGC AGCTCATTAA ACGCCGCCTG 
ATTAAGGGTG ACCAAGGACA GGCAGGGCCT CCAGGACCCC CTGGCCCTCC AGGCCCAAGA 
GGGCCACCTG GGGACACAGG GAAAGATGGC CCCCGTGGAA TGCCAGGAGT ACCCGGTGAA 
CCAGGAAAGC CAGGAGAACA AGGCTTGATG GGTCCTCTAG GGCCTCCGGG ACAAAAGGGT 
TCTATTGGAG CACCTGGAAT TCCAGGGATG AATGGGCAAA AGGGTGAGCC CGGGTTGCCT 
GGAGCAGTAG GACAGAATGG AATACCAGGA CCTAAGGGAG AACCTGGAGA ACAAGGTGAA 
AAGGGAGACG CTGGAGAGAA CGGCCCCAAG GGTGACACAG GCGAAAAGGG TGACCCTGGA 
TCATCTGCTG CAGGAATTAA GGGAGAACCT GGGGAATCTG GTCGTCCAGG GCAAAAGGGT 
GAACCAGGGC TTCCTGGGCT TCCTGGACTT CCGGGGATAA AGGGAGAACC AGGTTTCATT 
GGTCCTCAAG GAGAACCAGG CTTACCAGGT TTACCAGGAA CAAAAGGTGA ACGGGGGGAA 
GCAGGGCCTC CTGGAAGAGG TGAGCGAGGG GAACCTGGAG CCCCCGGACC AAAGGGGAAA 
CAAGGTGAAT CAGGAACTAG AGGCCCAAAG GGGTCAAAGG GGGATCGTGG AGAAAAAGGG 
GACTCTGGAG CTCAGGGACC AAGGGGTCCA CCTGGTCAAA AAGGGGATCA AGGAGCCACT 
GAGATCATAG ACTACAACGG CAACCTCCAC GAAGCCTTAC AGAGGATTAC CACCTTAACT 
GTCACGGGTC CCCCTGGACC TCCTGGACCT CAAGGACTAC AAGGGCCAAA GGGAGAGCAG 
GGATCTCCAG GAATCCCAGG AATGGATGGA GAGCAGGGAC TCAAAGGCTC AAAGGGAGAC 
ATGGGGGACC CAGGTATGAC AGGTGAAAAA GGAGGAATTG GACTTCCTGG ATTACCGGGA 
GCCAATGGAA TGAAAGGAGA AAAAGGAGAT TCTGGAATGC CGGGTCCACA GGGTCCTTCT 
ATCATAGGCC CACCAGGCCC ACCAGGTCCC CATGGCCCAC CTGGCCCCAT GGGACCTCAT 
GGACTTCCTG GACCAAAGGG TGAACCAGGG TTAAATGGTG TTAAAGGATT GAAGGGTGAA 
CCAGGTCAAA AGGGTGACAG AGGACCCCTT GGTCTTCCAG GAGCATCTGG CTTAGACGGA 
AAGCCAGGAT CCCGGGGTAC AGATGGTCCT ATGGGACCCC ATGGCCCTGC AGGTCCCAAA 
GGAGAAAGAG GTGAAAAAGG AGCTATGGGA GAGCCTGGAC CAAGAGGGCC CTATGGGCTG 
CCTGGCTTCC CTGGTCCACG GGGTGAGAAG GGTGATCTAG GAGAAAAGGG AGAAAAGGGA 
TTCCGTGGCG TTAAGGGGGA AAAAGGGGAG CCAGGCCAGC CTGGCCTGGA TGGGCTGGAT 
GCCCCTTGCC AATTGGGGCC AGATGGCTTA CCCATGCCTG GCTGTTGGCA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( XM_017008736.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864225.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X6, mRNA.

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CloneID	OHu95484
Clone ID Related Accession (Same CDS sequence)	XM_017008736.1
Accession Version	XM_017008736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGAG CATCTGGCTT AGACGGAAAG 
CCAGGATCCC GGGGTACAGA TGGTCCTATG GGACCCCATG GCCCTGCAGG TCCCAAAGGA 
GAAAGAGGTG AAAAAGGAGC TATGGGAGAG CCTGGACCAA GAGGGCCCTA TGGGCTGCCT 
GGCTTCCCTG GTCCACGGGG TGAGAAGGGT GATCTAGGAG AAAAGGGAGA AAAGGGATTC 
CGTGGCGTTA AGGGGGAAAA AGGGGAGCCA GGCCAGCCTG GCCTGGATGG GCTGGATGCC 
CCTTGCCAAT TGGGGCCAGA TGGCTTACCC ATGCCTGGCT GTTGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864225.1
CDS	719..2692
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGASGLDGKPGSRGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGFPGPRGEKGDLGEKGEKGFRGVKGEKGEPGQPGLDGLDAPCQLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	XM_017008736.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008736.1

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGAG CATCTGGCTT AGACGGAAAG 
CCAGGATCCC GGGGTACAGA TGGTCCTATG GGACCCCATG GCCCTGCAGG TCCCAAAGGA 
GAAAGAGGTG AAAAAGGAGC TATGGGAGAG CCTGGACCAA GAGGGCCCTA TGGGCTGCCT 
GGCTTCCCTG GTCCACGGGG TGAGAAGGGT GATCTAGGAG AAAAGGGAGA AAAGGGATTC 
CGTGGCGTTA AGGGGGAAAA AGGGGAGCCA GGCCAGCCTG GCCTGGATGG GCTGGATGCC 
CCTTGCCAAT TGGGGCCAGA TGGCTTACCC ATGCCTGGCT GTTGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( NM_032518.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115907.2 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 2, mRNA.

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CloneID	OHu02085
Clone ID Related Accession (Same CDS sequence)	NM_032518.3 , NM_032518.2
Accession Version	NM_032518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559923200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004051.1, AF293341.1 and BC030649.1. On May 31, 2019 this sequence version replaced NM_032518.2. Transcript Variant: This variant (2) lacks three 3' exons but includes an alternate 3' exon, resulting in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF293341.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGTGAC CTCTCCATCC 
CAGCATGTAC CTTGTTTGAT TCTTCTCCTG CTGTCTGCTT TGCTGTTCAG CTTGTGTGAT 
TCCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115907.2
CDS	547..2475
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKVTSPSQHVPCLILLLLSALLFSLCDSI

Target ORF information:

RefSeq Version	NM_032518.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032518.3

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGTGAC CTCTCCATCC 
CAGCATGTAC CTTGTTTGAT TCTTCTCCTG CTGTCTGCTT TGCTGTTCAG CTTGTGTGAT 
TCCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( NM_032518.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115907.2 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 2, mRNA.

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CloneID	OHu02085
Clone ID Related Accession (Same CDS sequence)	NM_032518.3 , NM_032518.2
Accession Version	NM_032518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549987200000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF293341.1 and BC030649.1. On Dec 1, 2003 this sequence version replaced NM_032518.1. Transcript Variant: This variant (2) lacks three 3' exons but includes an alternate 3' exon, resulting in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF293341.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGTGAC CTCTCCATCC 
CAGCATGTAC CTTGTTTGAT TCTTCTCCTG CTGTCTGCTT TGCTGTTCAG CTTGTGTGAT 
TCCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115907.2
CDS	532..2460
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKVTSPSQHVPCLILLLLSALLFSLCDSI

Target ORF information:

RefSeq Version	NM_032518.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032518.2

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGTGAC CTCTCCATCC 
CAGCATGTAC CTTGTTTGAT TCTTCTCCTG CTGTCTGCTT TGCTGTTCAG CTTGTGTGAT 
TCCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( NM_198721.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_942014.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 1, mRNA.

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CloneID	OHu11928
Clone ID Related Accession (Same CDS sequence)	NM_198721.4 , NM_198721.3
Accession Version	NM_198721.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1566316800000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004051.1, AF293341.1, BC030649.1, AF293340.1, AC097473.3 and KF457803.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_198721.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF293340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000399132.6/ ENSP00000382083.1 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGGATT CCGTGGCGTT 
AAGGGGGAAA AAGGGGAGCC AGGCCAGCCT GGCCTGGATG GGCTGGATGC CCCTTGCCAA 
TTGGGGCCAG ATGGCTTACC CATGCCTGGC TGTTGGCAAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942014.1
CDS	547..2511
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGFRGVKGEKGEPGQPGLDGLDAPCQLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	NM_198721.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198721.4

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGGATT CCGTGGCGTT 
AAGGGGGAAA AAGGGGAGCC AGGCCAGCCT GGCCTGGATG GGCTGGATGC CCCTTGCCAA 
TTGGGGCCAG ATGGCTTACC CATGCCTGGC TGTTGGCAAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL25A1 ( NM_198721.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_942014.1 Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11928
Clone ID Related Accession (Same CDS sequence)	NM_198721.4 , NM_198721.3
Accession Version	NM_198721.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	collagen alpha-1(XXV) chain isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF293341.1, BC030649.1, AF293340.1, AC097473.3 and KF457803.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2014 this sequence version replaced NM_198721.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF293340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGGATT CCGTGGCGTT 
AAGGGGGAAA AAGGGGAGCC AGGCCAGCCT GGCCTGGATG GGCTGGATGC CCCTTGCCAA 
TTGGGGCCAG ATGGCTTACC CATGCCTGGC TGTTGGCAAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942014.1
CDS	532..2496
Translation	MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAKGAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPPGQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDPGMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGTDGPMGPHGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGFRGVKGEKGEPGQPGLDGLDAPCQLGPDGLPMPGCWQK

Target ORF information:

RefSeq Version	NM_198721.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XXV alpha 1 chain (COL25A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198721.3

ORF Insert Sequence:

ATGCTGCTGA AGAAGCACGC AGGGAAAGGA GGGGGCCGGG AGCCCAGATC CGAGGACCCG 
ACCCCTGCCG AACAGCATTG TGCCCGGACC ATGCCCCCGT GTGCCGTCCT GGCGGCCCTC 
CTGTCAGTGG TGGCCGTGGT GTCTTGCCTG TACCTGGGTG TGAAAACCAA CGACCTCCAG 
GCGAGGATCG CCGCTCTCGA ATCCGCCAAA GGGGCCCCTT CCATTCATCT GCTGCCTGAT 
ACCCTGGATC ACCTCAAGAC TATGGTGCAA GAGAAAGTGG AGCGACTTCT GGCTCAGAAA 
TCCTATGAAC ATATGGCTAA AATAAGAATC GCAAGAGAAG CACCTTCAGA ATGTAACTGC 
CCAGCAGGCC CTCCAGGGAA ACGAGGTAAG AGAGGCCGAA GAGGAGAATC TGGTCCTCCT 
GGACAGCCTG GTCCTCAGGG CCCTCCTGGT CCAAAAGGCG ATAAGGGAGA ACAAGGTGAT 
CAGGGACCTA GGATGGTGTT TCCTAAAATC AATCATGGGT TTCTCTCTGC TGATCAGCAG 
CTCATTAAAC GCCGCCTGAT TAAGGGTGAC CAAGGACAGG CAGGGCCTCC AGGACCCCCT 
GGCCCTCCAG GCCCAAGAGG GCCACCTGGG GACACAGGGA AAGATGGCCC CCGTGGAATG 
CCAGGAGTAC CCGGTGAACC AGGAAAGCCA GGAGAACAAG GCTTGATGGG TCCTCTAGGG 
CCTCCGGGAC AAAAGGGTTC TATTGGAGCA CCTGGAATTC CAGGGATGAA TGGGCAAAAG 
GGTGAGCCCG GGTTGCCTGG AGCAGTAGGA CAGAATGGAA TACCAGGACC TAAGGGAGAA 
CCTGGAGAAC AAGGTGAAAA GGGAGACGCT GGAGAGAACG GCCCCAAGGG TGACACAGGC 
GAAAAGGGTG ACCCTGGATC ATCTGCTGCA GGAATTAAGG GAGAACCTGG GGAATCTGGT 
CGTCCAGGGC AAAAGGGTGA ACCAGGGCTT CCTGGGCTTC CTGGACTTCC GGGGATAAAG 
GGAGAACCAG GTTTCATTGG TCCTCAAGGA GAACCAGGCT TACCAGGTTT ACCAGGAACA 
AAAGGTGAAC GGGGGGAAGC AGGGCCTCCT GGAAGAGGTG AGCGAGGGGA ACCTGGAGCC 
CCCGGACCAA AGGGGAAACA AGGTGAATCA GGAACTAGAG GCCCAAAGGG GTCAAAGGGG 
GATCGTGGAG AAAAAGGGGA CTCTGGAGCT CAGGGACCAA GGGGTCCACC TGGTCAAAAA 
GGGGATCAAG GAGCCACTGA GATCATAGAC TACAACGGCA ACCTCCACGA AGCCTTACAG 
AGGATTACCA CCTTAACTGT CACGGGTCCC CCTGGACCTC CTGGACCTCA AGGACTACAA 
GGGCCAAAGG GAGAGCAGGG ATCTCCAGGA ATCCCAGGAA TGGATGGAGA GCAGGGACTC 
AAAGGCTCAA AGGGAGACAT GGGGGACCCA GGTATGACAG GTGAAAAAGG AGGAATTGGA 
CTTCCTGGAT TACCGGGAGC CAATGGAATG AAAGGAGAAA AAGGAGATTC TGGAATGCCG 
GGTCCACAGG GTCCTTCTAT CATAGGCCCA CCAGGCCCAC CAGGTCCCCA TGGCCCACCT 
GGCCCCATGG GACCTCATGG ACTTCCTGGA CCAAAGGGTA CAGATGGTCC TATGGGACCC 
CATGGCCCTG CAGGTCCCAA AGGAGAAAGA GGTGAAAAAG GAGCTATGGG AGAGCCTGGA 
CCAAGAGGGC CCTATGGGCT GCCTGGGAAA GATGGAGAGC CTGGTCTTGA TGGCTTCCCT 
GGTCCACGGG GTGAGAAGGG TGATCTAGGA GAAAAGGGAG AAAAGGGATT CCGTGGCGTT 
AAGGGGGAAA AAGGGGAGCC AGGCCAGCCT GGCCTGGATG GGCTGGATGC CCCTTGCCAA 
TTGGGGCCAG ATGGCTTACC CATGCCTGGC TGTTGGCAAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL25A1
Entrez Gene ID	84570
Full Name	collagen type XXV alpha 1 chain
Synonyms	AMY,CFEOM5,CLAC,CLAC-P,CLACP
General protein information	Preferred Names collagen type XXV alpha 1 chain Names collagen alpha-1(XXV) chain alzheimer disease amyloid-associated protein collagen-like Alzheimer amyloid plaque component collagenous Alzheimer amyloid plaque component
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q25
Summary	This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice, however, found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011].
Disorder MIM:	610004
Disorder Html:	Fibrosis of extraocular muscles, congenital, 5, 616219 (3)

Pan troglodytes (chimpanzee)	COL25A1	XP_001137848.1
Bos taurus (cattle)	COL25A1	XP_002688152.2
Mus musculus (house mouse)	Col25a1	NP_084114.2
Xenopus tropicalis (tropical clawed frog)	col25a1.2	NP_001123747.1
Macaca mulatta (Rhesus monkey)	COL25A1	XP_001087828.1
Gallus gallus (chicken)	COL25A1	XP_427455.4
Canis lupus familiaris (dog)	COL25A1	XP_005639323.1
Homo sapiens (human)	COL25A1	NP_942014.1
Danio rerio (zebrafish)	LOC101882764	XP_005166599.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL25A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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