TAS1R2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TAS1R2
Entrez Gene ID	80834
Full Name	taste 1 receptor member 2
Synonyms	GPR71,T1R2,TR2
General protein information	Preferred Names taste 1 receptor member 2 Names taste receptor type 1 member 2 G-protein coupled receptor 71 sweet taste receptor T1R2 taste receptor, type 1, member 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.13
Disorder MIM:	606226

mRNA and Protein(s)

mRNA	Protein	Name
NM_152232.3	NP_689418.2	taste receptor type 1 member 2 precursor
NM_152232.2	NP_689418.2	taste receptor type 1 member 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TAS1R2	NP_689418.2
Macaca mulatta (Rhesus monkey)	TAS1R2	NP_001121561.1
Bos taurus (cattle)	TAS1R2	NP_001193458.1
Mus musculus (house mouse)	Tas1r2	NP_114079.1
Canis lupus familiaris (dog)	TAS1R2	NP_001026989.1
Rattus norvegicus (Norway rat)	Tas1r2	NP_001258195.1
Pan troglodytes (chimpanzee)	LOC100615078	XP_003949358.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits.
Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E
The Journal of biological chemistry294(13)4759-4774(2019 03)

Single Nucleotide Polymorphisms in Taste Receptor Genes Are Associated with Snacking Patterns of Preschool-Aged Children in the Guelph Family Health Study: A Pilot Study.
Chamoun E, Hutchinson JM, Krystia O, Mirotta JA, Mutch DM, Buchholz AC, Duncan AM, Darlington G, Haines J, Ma DWL,
Nutrients10(2)(2018 Jan)

Salivary leptin and TAS1R2/TAS1R3 polymorphisms are related to sweet taste sensitivity and carbohydrate intake from a buffet meal in healthy young adults.
Han P, Keast RSJ, Roura E
The British journal of nutrition118(10)763-770(2017 Nov)

Activation mechanism of the G protein-coupled sweet receptor heterodimer with sweeteners and allosteric agonists.
Kim SK, Chen Y, Abrol R, Goddard WA 3rd, Guthrie B
Proceedings of the National Academy of Sciences of the United States of America114(10)2568-2573(2017 03)

The receptors and cells for mammalian taste.
Chandrashekar J, Hoon MA, Ryba NJ, Zuker CS
Nature444(7117)288-94(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TAS1R2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TAS1R2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17326	NM_152232.3 Latest version!	Homo sapiens taste 1 receptor member 2 (TAS1R2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
View Old Accession Versions >>
OHu17326	NM_152232.2	Homo sapiens taste 1 receptor member 2 (TAS1R2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TAS1R2 ( NM_152232.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689418.2 Homo sapiens taste 1 receptor member 2 (TAS1R2), mRNA.

Price & Availability↑

CloneID	OHu17326
Clone ID Related Accession (Same CDS sequence)	NM_152232.3 , NM_152232.2
Accession Version	NM_152232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX537160.6 and AL831755.9. On Nov 27, 2019 this sequence version replaced NM_152232.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000151.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGGGCCCA GGGCAAAGAC CATCTCCTCC CTGTTCTTCC TCCTATGGGT CCTGGCTGAG 
CCGGCTGAGA ACTCGGACTT CTACCTGCCT GGGGATTACC TCCTGGGTGG CCTCTTCTCC 
CTCCATGCCA ACATGAAGGG CATTGTTCAC CTTAACTTCC TGCAGGTGCC CATGTGCAAG 
GAGTATGAAG TGAAGGTGAT AGGCTACAAC CTCATGCAGG CCATGCGCTT TGCGGTGGAG 
GAGATCAACA ATGACAGCAG CCTGCTGCCT GGTGTGCTGC TGGGCTATGA GATCGTGGAT 
GTGTGCTACA TCTCCAACAA TGTCCAGCCG GTGCTCTACT TCCTGGCACA CGAGGACAAC 
CTCCTTCCCA TCCAAGAGGA CTACAGTAAC TACATTTCCC GTGTGGTGGC TGTCATTGGC 
CCTGACAACT CCGAGTCTGT CATGACTGTG GCCAACTTCC TCTCCCTATT TCTCCTTCCA 
CAGATCACCT ACAGCGCCAT CAGCGATGAG CTGCGAGACA AGGTGCGCTT CCCGGCTTTG 
CTGCGTACCA CACCCAGCGC CGACCACCAC ATCGAGGCCA TGGTGCAGCT GATGCTGCAC 
TTCCGCTGGA ACTGGATCAT TGTGCTGGTG AGCAGCGACA CCTATGGCCG CGACAATGGC 
CAGCTGCTTG GCGAGCGCGT GGCCCGGCGC GACATCTGCA TCGCCTTCCA GGAGACGCTG 
CCCACACTGC AGCCCAACCA GAACATGACG TCAGAGGAGC GCCAGCGCCT GGTGACCATT 
GTGGACAAGC TGCAGCAGAG CACAGCGCGC GTCGTGGTCG TGTTCTCGCC CGACCTGACC 
CTGTACCACT TCTTCAATGA GGTGCTGCGC CAGAACTTCA CTGGCGCCGT GTGGATCGCC 
TCCGAGTCCT GGGCCATCGA CCCGGTCCTG CACAACCTCA CGGAGCTGCG CCACTTGGGC 
ACCTTCCTGG GCATCACCAT CCAGAGCGTG CCCATCCCGG GCTTCAGTGA GTTCCGCGAG 
TGGGGCCCAC AGGCTGGGCC GCCACCCCTC AGCAGGACCA GCCAGAGCTA TACCTGCAAC 
CAGGAGTGCG ACAACTGCCT GAACGCCACC TTGTCCTTCA ACACCATTCT CAGGCTCTCT 
GGGGAGCGTG TCGTCTACAG CGTGTACTCT GCGGTCTATG CTGTGGCCCA TGCCCTGCAC 
AGCCTCCTCG GCTGTGACAA AAGCACCTGC ACCAAGAGGG TGGTCTACCC CTGGCAGCTG 
CTTGAGGAGA TCTGGAAGGT CAACTTCACT CTCCTGGACC ACCAAATCTT CTTCGACCCG 
CAAGGGGACG TGGCTCTGCA CTTGGAGATT GTCCAGTGGC AATGGGACCG GAGCCAGAAT 
CCCTTCCAGA GCGTCGCCTC CTACTACCCC CTGCAGCGAC AGCTGAAGAA CATCCAAGAC 
ATCTCCTGGC ACACCATCAA CAACACGATC CCTATGTCCA TGTGTTCCAA GAGGTGCCAG 
TCAGGGCAAA AGAAGAAGCC TGTGGGCATC CACGTCTGCT GCTTCGAGTG CATCGACTGC 
CTTCCCGGCA CCTTCCTCAA CCACACTGAA GATGAATATG AATGCCAGGC CTGCCCGAAT 
AACGAGTGGT CCTACCAGAG TGAGACCTCC TGCTTCAAGC GGCAGCTGGT CTTCCTGGAA 
TGGCATGAGG CACCCACCAT CGCTGTGGCC CTGCTGGCCG CCCTGGGCTT CCTCAGCACC 
CTGGCCATCC TGGTGATATT CTGGAGGCAC TTCCAGACAC CCATAGTTCG CTCGGCTGGG 
GGCCCCATGT GCTTCCTGAT GCTGACACTG CTGCTGGTGG CATACATGGT GGTCCCGGTG 
TACGTGGGGC CGCCCAAGGT CTCCACCTGC CTCTGCCGCC AGGCCCTCTT TCCCCTCTGC 
TTCACAATCT GCATCTCCTG TATCGCCGTG CGTTCTTTCC AGATCGTCTG CGCCTTCAAG 
ATGGCCAGCC GCTTCCCACG CGCCTACAGC TACTGGGTCC GCTACCAGGG GCCCTACGTC 
TCTATGGCAT TTATCACGGT ACTCAAAATG GTCATTGTGG TAATTGGCAT GCTGGCCACG 
GGCCTCAGTC CCACCACCCG TACTGACCCC GATGACCCCA AGATCACAAT TGTCTCCTGT 
AACCCCAACT ACCGCAACAG CCTGCTGTTC AACACCAGCC TGGACCTGCT GCTCTCAGTG 
GTGGGTTTCA GCTTCGCCTA CATGGGCAAA GAGCTGCCCA CCAACTACAA CGAGGCCAAG 
TTCATCACCC TCAGCATGAC CTTCTATTTC ACCTCATCCG TCTCCCTCTG CACCTTCATG 
TCTGCCTACA GCGGGGTGCT GGTCACCATC GTGGACCTCT TGGTCACTGT GCTCAACCTC 
CTGGCCATCA GCCTGGGCTA CTTCGGCCCC AAGTGCTACA TGATCCTCTT CTACCCGGAG 
CGCAACACGC CCGCCTACTT CAACAGCATG ATCCAGGGCT ACACCATGAG GAGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689418.2
CDS	688..3207
Translation	MGPRAKTISSLFFLLWVLAEPAENSDFYLPGDYLLGGLFSLHANMKGIVHLNFLQVPMCKEYEVKVIGYNLMQAMRFAVEEINNDSSLLPGVLLGYEIVDVCYISNNVQPVLYFLAHEDNLLPIQEDYSNYISRVVAVIGPDNSESVMTVANFLSLFLLPQITYSAISDELRDKVRFPALLRTTPSADHHIEAMVQLMLHFRWNWIIVLVSSDTYGRDNGQLLGERVARRDICIAFQETLPTLQPNQNMTSEERQRLVTIVDKLQQSTARVVVVFSPDLTLYHFFNEVLRQNFTGAVWIASESWAIDPVLHNLTELRHLGTFLGITIQSVPIPGFSEFREWGPQAGPPPLSRTSQSYTCNQECDNCLNATLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDKSTCTKRVVYPWQLLEEIWKVNFTLLDHQIFFDPQGDVALHLEIVQWQWDRSQNPFQSVASYYPLQRQLKNIQDISWHTINNTIPMSMCSKRCQSGQKKKPVGIHVCCFECIDCLPGTFLNHTEDEYECQACPNNEWSYQSETSCFKRQLVFLEWHEAPTIAVALLAALGFLSTLAILVIFWRHFQTPIVRSAGGPMCFLMLTLLLVAYMVVPVYVGPPKVSTCLCRQALFPLCFTICISCIAVRSFQIVCAFKMASRFPRAYSYWVRYQGPYVSMAFITVLKMVIVVIGMLATGLSPTTRTDPDDPKITIVSCNPNYRNSLLFNTSLDLLLSVVGFSFAYMGKELPTNYNEAKFITLSMTFYFTSSVSLCTFMSAYSGVLVTIVDLLVTVLNLLAISLGYFGPKCYMILFYPERNTPAYFNSMIQGYTMRRD

Target ORF information:

RefSeq Version	NM_152232.3
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 2 (TAS1R2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152232.3

ORF Insert Sequence:

ATGGGGCCCA GGGCAAAGAC CATCTCCTCC CTGTTCTTCC TCCTATGGGT CCTGGCTGAG 
CCGGCTGAGA ACTCGGACTT CTACCTGCCT GGGGATTACC TCCTGGGTGG CCTCTTCTCC 
CTCCATGCCA ACATGAAGGG CATTGTTCAC CTTAACTTCC TGCAGGTGCC CATGTGCAAG 
GAGTATGAAG TGAAGGTGAT AGGCTACAAC CTCATGCAGG CCATGCGCTT TGCGGTGGAG 
GAGATCAACA ATGACAGCAG CCTGCTGCCT GGTGTGCTGC TGGGCTATGA GATCGTGGAT 
GTGTGCTACA TCTCCAACAA TGTCCAGCCG GTGCTCTACT TCCTGGCACA CGAGGACAAC 
CTCCTTCCCA TCCAAGAGGA CTACAGTAAC TACATTTCCC GTGTGGTGGC TGTCATTGGC 
CCTGACAACT CCGAGTCTGT CATGACTGTG GCCAACTTCC TCTCCCTATT TCTCCTTCCA 
CAGATCACCT ACAGCGCCAT CAGCGATGAG CTGCGAGACA AGGTGCGCTT CCCGGCTTTG 
CTGCGTACCA CACCCAGCGC CGACCACCAC ATCGAGGCCA TGGTGCAGCT GATGCTGCAC 
TTCCGCTGGA ACTGGATCAT TGTGCTGGTG AGCAGCGACA CCTATGGCCG CGACAATGGC 
CAGCTGCTTG GCGAGCGCGT GGCCCGGCGC GACATCTGCA TCGCCTTCCA GGAGACGCTG 
CCCACACTGC AGCCCAACCA GAACATGACG TCAGAGGAGC GCCAGCGCCT GGTGACCATT 
GTGGACAAGC TGCAGCAGAG CACAGCGCGC GTCGTGGTCG TGTTCTCGCC CGACCTGACC 
CTGTACCACT TCTTCAATGA GGTGCTGCGC CAGAACTTCA CTGGCGCCGT GTGGATCGCC 
TCCGAGTCCT GGGCCATCGA CCCGGTCCTG CACAACCTCA CGGAGCTGCG CCACTTGGGC 
ACCTTCCTGG GCATCACCAT CCAGAGCGTG CCCATCCCGG GCTTCAGTGA GTTCCGCGAG 
TGGGGCCCAC AGGCTGGGCC GCCACCCCTC AGCAGGACCA GCCAGAGCTA TACCTGCAAC 
CAGGAGTGCG ACAACTGCCT GAACGCCACC TTGTCCTTCA ACACCATTCT CAGGCTCTCT 
GGGGAGCGTG TCGTCTACAG CGTGTACTCT GCGGTCTATG CTGTGGCCCA TGCCCTGCAC 
AGCCTCCTCG GCTGTGACAA AAGCACCTGC ACCAAGAGGG TGGTCTACCC CTGGCAGCTG 
CTTGAGGAGA TCTGGAAGGT CAACTTCACT CTCCTGGACC ACCAAATCTT CTTCGACCCG 
CAAGGGGACG TGGCTCTGCA CTTGGAGATT GTCCAGTGGC AATGGGACCG GAGCCAGAAT 
CCCTTCCAGA GCGTCGCCTC CTACTACCCC CTGCAGCGAC AGCTGAAGAA CATCCAAGAC 
ATCTCCTGGC ACACCATCAA CAACACGATC CCTATGTCCA TGTGTTCCAA GAGGTGCCAG 
TCAGGGCAAA AGAAGAAGCC TGTGGGCATC CACGTCTGCT GCTTCGAGTG CATCGACTGC 
CTTCCCGGCA CCTTCCTCAA CCACACTGAA GATGAATATG AATGCCAGGC CTGCCCGAAT 
AACGAGTGGT CCTACCAGAG TGAGACCTCC TGCTTCAAGC GGCAGCTGGT CTTCCTGGAA 
TGGCATGAGG CACCCACCAT CGCTGTGGCC CTGCTGGCCG CCCTGGGCTT CCTCAGCACC 
CTGGCCATCC TGGTGATATT CTGGAGGCAC TTCCAGACAC CCATAGTTCG CTCGGCTGGG 
GGCCCCATGT GCTTCCTGAT GCTGACACTG CTGCTGGTGG CATACATGGT GGTCCCGGTG 
TACGTGGGGC CGCCCAAGGT CTCCACCTGC CTCTGCCGCC AGGCCCTCTT TCCCCTCTGC 
TTCACAATCT GCATCTCCTG TATCGCCGTG CGTTCTTTCC AGATCGTCTG CGCCTTCAAG 
ATGGCCAGCC GCTTCCCACG CGCCTACAGC TACTGGGTCC GCTACCAGGG GCCCTACGTC 
TCTATGGCAT TTATCACGGT ACTCAAAATG GTCATTGTGG TAATTGGCAT GCTGGCCACG 
GGCCTCAGTC CCACCACCCG TACTGACCCC GATGACCCCA AGATCACAAT TGTCTCCTGT 
AACCCCAACT ACCGCAACAG CCTGCTGTTC AACACCAGCC TGGACCTGCT GCTCTCAGTG 
GTGGGTTTCA GCTTCGCCTA CATGGGCAAA GAGCTGCCCA CCAACTACAA CGAGGCCAAG 
TTCATCACCC TCAGCATGAC CTTCTATTTC ACCTCATCCG TCTCCCTCTG CACCTTCATG 
TCTGCCTACA GCGGGGTGCT GGTCACCATC GTGGACCTCT TGGTCACTGT GCTCAACCTC 
CTGGCCATCA GCCTGGGCTA CTTCGGCCCC AAGTGCTACA TGATCCTCTT CTACCCGGAG 
CGCAACACGC CCGCCTACTT CAACAGCATG ATCCAGGGCT ACACCATGAG GAGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R2 ( NM_152232.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689418.2 Homo sapiens taste 1 receptor member 2 (TAS1R2), mRNA.

Price & Availability↑

CloneID	OHu17326
Clone ID Related Accession (Same CDS sequence)	NM_152232.3 , NM_152232.2
Accession Version	NM_152232.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556553600000
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX537160.6 and AL831755.9. On Aug 18, 2006 this sequence version replaced NM_152232.1. Sequence Note: This sequence is assembled by in silico methods with additional support of similarity to the human protein contained in BK000151.1 and the mouse proteins contained in AF337041.1 and AY032623.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000151.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2151887 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGGGCCCA GGGCAAAGAC CATCTCCTCC CTGTTCTTCC TCCTATGGGT CCTGGCTGAG 
CCGGCTGAGA ACTCGGACTT CTACCTGCCT GGGGATTACC TCCTGGGTGG CCTCTTCTCC 
CTCCATGCCA ACATGAAGGG CATTGTTCAC CTTAACTTCC TGCAGGTGCC CATGTGCAAG 
GAGTATGAAG TGAAGGTGAT AGGCTACAAC CTCATGCAGG CCATGCGCTT TGCGGTGGAG 
GAGATCAACA ATGACAGCAG CCTGCTGCCT GGTGTGCTGC TGGGCTATGA GATCGTGGAT 
GTGTGCTACA TCTCCAACAA TGTCCAGCCG GTGCTCTACT TCCTGGCACA CGAGGACAAC 
CTCCTTCCCA TCCAAGAGGA CTACAGTAAC TACATTTCCC GTGTGGTGGC TGTCATTGGC 
CCTGACAACT CCGAGTCTGT CATGACTGTG GCCAACTTCC TCTCCCTATT TCTCCTTCCA 
CAGATCACCT ACAGCGCCAT CAGCGATGAG CTGCGAGACA AGGTGCGCTT CCCGGCTTTG 
CTGCGTACCA CACCCAGCGC CGACCACCAC ATCGAGGCCA TGGTGCAGCT GATGCTGCAC 
TTCCGCTGGA ACTGGATCAT TGTGCTGGTG AGCAGCGACA CCTATGGCCG CGACAATGGC 
CAGCTGCTTG GCGAGCGCGT GGCCCGGCGC GACATCTGCA TCGCCTTCCA GGAGACGCTG 
CCCACACTGC AGCCCAACCA GAACATGACG TCAGAGGAGC GCCAGCGCCT GGTGACCATT 
GTGGACAAGC TGCAGCAGAG CACAGCGCGC GTCGTGGTCG TGTTCTCGCC CGACCTGACC 
CTGTACCACT TCTTCAATGA GGTGCTGCGC CAGAACTTCA CTGGCGCCGT GTGGATCGCC 
TCCGAGTCCT GGGCCATCGA CCCGGTCCTG CACAACCTCA CGGAGCTGCG CCACTTGGGC 
ACCTTCCTGG GCATCACCAT CCAGAGCGTG CCCATCCCGG GCTTCAGTGA GTTCCGCGAG 
TGGGGCCCAC AGGCTGGGCC GCCACCCCTC AGCAGGACCA GCCAGAGCTA TACCTGCAAC 
CAGGAGTGCG ACAACTGCCT GAACGCCACC TTGTCCTTCA ACACCATTCT CAGGCTCTCT 
GGGGAGCGTG TCGTCTACAG CGTGTACTCT GCGGTCTATG CTGTGGCCCA TGCCCTGCAC 
AGCCTCCTCG GCTGTGACAA AAGCACCTGC ACCAAGAGGG TGGTCTACCC CTGGCAGCTG 
CTTGAGGAGA TCTGGAAGGT CAACTTCACT CTCCTGGACC ACCAAATCTT CTTCGACCCG 
CAAGGGGACG TGGCTCTGCA CTTGGAGATT GTCCAGTGGC AATGGGACCG GAGCCAGAAT 
CCCTTCCAGA GCGTCGCCTC CTACTACCCC CTGCAGCGAC AGCTGAAGAA CATCCAAGAC 
ATCTCCTGGC ACACCATCAA CAACACGATC CCTATGTCCA TGTGTTCCAA GAGGTGCCAG 
TCAGGGCAAA AGAAGAAGCC TGTGGGCATC CACGTCTGCT GCTTCGAGTG CATCGACTGC 
CTTCCCGGCA CCTTCCTCAA CCACACTGAA GATGAATATG AATGCCAGGC CTGCCCGAAT 
AACGAGTGGT CCTACCAGAG TGAGACCTCC TGCTTCAAGC GGCAGCTGGT CTTCCTGGAA 
TGGCATGAGG CACCCACCAT CGCTGTGGCC CTGCTGGCCG CCCTGGGCTT CCTCAGCACC 
CTGGCCATCC TGGTGATATT CTGGAGGCAC TTCCAGACAC CCATAGTTCG CTCGGCTGGG 
GGCCCCATGT GCTTCCTGAT GCTGACACTG CTGCTGGTGG CATACATGGT GGTCCCGGTG 
TACGTGGGGC CGCCCAAGGT CTCCACCTGC CTCTGCCGCC AGGCCCTCTT TCCCCTCTGC 
TTCACAATCT GCATCTCCTG TATCGCCGTG CGTTCTTTCC AGATCGTCTG CGCCTTCAAG 
ATGGCCAGCC GCTTCCCACG CGCCTACAGC TACTGGGTCC GCTACCAGGG GCCCTACGTC 
TCTATGGCAT TTATCACGGT ACTCAAAATG GTCATTGTGG TAATTGGCAT GCTGGCCACG 
GGCCTCAGTC CCACCACCCG TACTGACCCC GATGACCCCA AGATCACAAT TGTCTCCTGT 
AACCCCAACT ACCGCAACAG CCTGCTGTTC AACACCAGCC TGGACCTGCT GCTCTCAGTG 
GTGGGTTTCA GCTTCGCCTA CATGGGCAAA GAGCTGCCCA CCAACTACAA CGAGGCCAAG 
TTCATCACCC TCAGCATGAC CTTCTATTTC ACCTCATCCG TCTCCCTCTG CACCTTCATG 
TCTGCCTACA GCGGGGTGCT GGTCACCATC GTGGACCTCT TGGTCACTGT GCTCAACCTC 
CTGGCCATCA GCCTGGGCTA CTTCGGCCCC AAGTGCTACA TGATCCTCTT CTACCCGGAG 
CGCAACACGC CCGCCTACTT CAACAGCATG ATCCAGGGCT ACACCATGAG GAGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689418.2
CDS	2..2521
Translation	MGPRAKTISSLFFLLWVLAEPAENSDFYLPGDYLLGGLFSLHANMKGIVHLNFLQVPMCKEYEVKVIGYNLMQAMRFAVEEINNDSSLLPGVLLGYEIVDVCYISNNVQPVLYFLAHEDNLLPIQEDYSNYISRVVAVIGPDNSESVMTVANFLSLFLLPQITYSAISDELRDKVRFPALLRTTPSADHHIEAMVQLMLHFRWNWIIVLVSSDTYGRDNGQLLGERVARRDICIAFQETLPTLQPNQNMTSEERQRLVTIVDKLQQSTARVVVVFSPDLTLYHFFNEVLRQNFTGAVWIASESWAIDPVLHNLTELRHLGTFLGITIQSVPIPGFSEFREWGPQAGPPPLSRTSQSYTCNQECDNCLNATLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDKSTCTKRVVYPWQLLEEIWKVNFTLLDHQIFFDPQGDVALHLEIVQWQWDRSQNPFQSVASYYPLQRQLKNIQDISWHTINNTIPMSMCSKRCQSGQKKKPVGIHVCCFECIDCLPGTFLNHTEDEYECQACPNNEWSYQSETSCFKRQLVFLEWHEAPTIAVALLAALGFLSTLAILVIFWRHFQTPIVRSAGGPMCFLMLTLLLVAYMVVPVYVGPPKVSTCLCRQALFPLCFTICISCIAVRSFQIVCAFKMASRFPRAYSYWVRYQGPYVSMAFITVLKMVIVVIGMLATGLSPTTRTDPDDPKITIVSCNPNYRNSLLFNTSLDLLLSVVGFSFAYMGKELPTNYNEAKFITLSMTFYFTSSVSLCTFMSAYSGVLVTIVDLLVTVLNLLAISLGYFGPKCYMILFYPERNTPAYFNSMIQGYTMRRD

Target ORF information:

RefSeq Version	NM_152232.2
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 2 (TAS1R2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152232.2

ORF Insert Sequence:

ATGGGGCCCA GGGCAAAGAC CATCTCCTCC CTGTTCTTCC TCCTATGGGT CCTGGCTGAG 
CCGGCTGAGA ACTCGGACTT CTACCTGCCT GGGGATTACC TCCTGGGTGG CCTCTTCTCC 
CTCCATGCCA ACATGAAGGG CATTGTTCAC CTTAACTTCC TGCAGGTGCC CATGTGCAAG 
GAGTATGAAG TGAAGGTGAT AGGCTACAAC CTCATGCAGG CCATGCGCTT TGCGGTGGAG 
GAGATCAACA ATGACAGCAG CCTGCTGCCT GGTGTGCTGC TGGGCTATGA GATCGTGGAT 
GTGTGCTACA TCTCCAACAA TGTCCAGCCG GTGCTCTACT TCCTGGCACA CGAGGACAAC 
CTCCTTCCCA TCCAAGAGGA CTACAGTAAC TACATTTCCC GTGTGGTGGC TGTCATTGGC 
CCTGACAACT CCGAGTCTGT CATGACTGTG GCCAACTTCC TCTCCCTATT TCTCCTTCCA 
CAGATCACCT ACAGCGCCAT CAGCGATGAG CTGCGAGACA AGGTGCGCTT CCCGGCTTTG 
CTGCGTACCA CACCCAGCGC CGACCACCAC ATCGAGGCCA TGGTGCAGCT GATGCTGCAC 
TTCCGCTGGA ACTGGATCAT TGTGCTGGTG AGCAGCGACA CCTATGGCCG CGACAATGGC 
CAGCTGCTTG GCGAGCGCGT GGCCCGGCGC GACATCTGCA TCGCCTTCCA GGAGACGCTG 
CCCACACTGC AGCCCAACCA GAACATGACG TCAGAGGAGC GCCAGCGCCT GGTGACCATT 
GTGGACAAGC TGCAGCAGAG CACAGCGCGC GTCGTGGTCG TGTTCTCGCC CGACCTGACC 
CTGTACCACT TCTTCAATGA GGTGCTGCGC CAGAACTTCA CTGGCGCCGT GTGGATCGCC 
TCCGAGTCCT GGGCCATCGA CCCGGTCCTG CACAACCTCA CGGAGCTGCG CCACTTGGGC 
ACCTTCCTGG GCATCACCAT CCAGAGCGTG CCCATCCCGG GCTTCAGTGA GTTCCGCGAG 
TGGGGCCCAC AGGCTGGGCC GCCACCCCTC AGCAGGACCA GCCAGAGCTA TACCTGCAAC 
CAGGAGTGCG ACAACTGCCT GAACGCCACC TTGTCCTTCA ACACCATTCT CAGGCTCTCT 
GGGGAGCGTG TCGTCTACAG CGTGTACTCT GCGGTCTATG CTGTGGCCCA TGCCCTGCAC 
AGCCTCCTCG GCTGTGACAA AAGCACCTGC ACCAAGAGGG TGGTCTACCC CTGGCAGCTG 
CTTGAGGAGA TCTGGAAGGT CAACTTCACT CTCCTGGACC ACCAAATCTT CTTCGACCCG 
CAAGGGGACG TGGCTCTGCA CTTGGAGATT GTCCAGTGGC AATGGGACCG GAGCCAGAAT 
CCCTTCCAGA GCGTCGCCTC CTACTACCCC CTGCAGCGAC AGCTGAAGAA CATCCAAGAC 
ATCTCCTGGC ACACCATCAA CAACACGATC CCTATGTCCA TGTGTTCCAA GAGGTGCCAG 
TCAGGGCAAA AGAAGAAGCC TGTGGGCATC CACGTCTGCT GCTTCGAGTG CATCGACTGC 
CTTCCCGGCA CCTTCCTCAA CCACACTGAA GATGAATATG AATGCCAGGC CTGCCCGAAT 
AACGAGTGGT CCTACCAGAG TGAGACCTCC TGCTTCAAGC GGCAGCTGGT CTTCCTGGAA 
TGGCATGAGG CACCCACCAT CGCTGTGGCC CTGCTGGCCG CCCTGGGCTT CCTCAGCACC 
CTGGCCATCC TGGTGATATT CTGGAGGCAC TTCCAGACAC CCATAGTTCG CTCGGCTGGG 
GGCCCCATGT GCTTCCTGAT GCTGACACTG CTGCTGGTGG CATACATGGT GGTCCCGGTG 
TACGTGGGGC CGCCCAAGGT CTCCACCTGC CTCTGCCGCC AGGCCCTCTT TCCCCTCTGC 
TTCACAATCT GCATCTCCTG TATCGCCGTG CGTTCTTTCC AGATCGTCTG CGCCTTCAAG 
ATGGCCAGCC GCTTCCCACG CGCCTACAGC TACTGGGTCC GCTACCAGGG GCCCTACGTC 
TCTATGGCAT TTATCACGGT ACTCAAAATG GTCATTGTGG TAATTGGCAT GCTGGCCACG 
GGCCTCAGTC CCACCACCCG TACTGACCCC GATGACCCCA AGATCACAAT TGTCTCCTGT 
AACCCCAACT ACCGCAACAG CCTGCTGTTC AACACCAGCC TGGACCTGCT GCTCTCAGTG 
GTGGGTTTCA GCTTCGCCTA CATGGGCAAA GAGCTGCCCA CCAACTACAA CGAGGCCAAG 
TTCATCACCC TCAGCATGAC CTTCTATTTC ACCTCATCCG TCTCCCTCTG CACCTTCATG 
TCTGCCTACA GCGGGGTGCT GGTCACCATC GTGGACCTCT TGGTCACTGT GCTCAACCTC 
CTGGCCATCA GCCTGGGCTA CTTCGGCCCC AAGTGCTACA TGATCCTCTT CTACCCGGAG 
CGCAACACGC CCGCCTACTT CAACAGCATG ATCCAGGGCT ACACCATGAG GAGGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits.
The Journal of biological chemistry294(13)4759-4774(2019 03)
Park J,Selvam B,Sanematsu K,Shigemura N,Shukla D,Procko E

Single Nucleotide Polymorphisms in Taste Receptor Genes Are Associated with Snacking Patterns of Preschool-Aged Children in the Guelph Family Health Study: A Pilot Study.
Nutrients10(2)(2018 Jan)
Chamoun E,Hutchinson JM,Krystia O,Mirotta JA,Mutch DM,Buchholz AC,Duncan AM,Darlington G,Haines J,Ma DWL,

Salivary leptin and TAS1R2/TAS1R3 polymorphisms are related to sweet taste sensitivity and carbohydrate intake from a buffet meal in healthy young adults.
The British journal of nutrition118(10)763-770(2017 Nov)
Han P,Keast RSJ,Roura E

Activation mechanism of the G protein-coupled sweet receptor heterodimer with sweeteners and allosteric agonists.
Proceedings of the National Academy of Sciences of the United States of America114(10)2568-2573(2017 03)
Kim SK,Chen Y,Abrol R,Goddard WA 3rd,Guthrie B

The receptors and cells for mammalian taste.
Nature444(7117)288-94(2006 Nov)
Chandrashekar J,Hoon MA,Ryba NJ,Zuker CS

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TAS1R2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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