SLC44A4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC44A4
Entrez Gene ID	80736
Full Name	solute carrier family 44 member 4
Synonyms	C6orf29,CTL4,DFNA72,NG22,TPPT,hTPPT1
General protein information	Preferred Names solute carrier family 44 member 4 Names choline transporter-like protein 4 testicular tissue protein Li 48 thiamine pyrophosphate transporter thiamine pyrophosphate transporter 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	The protein encoded by this gene may be a sodium-dependent transmembrane transport protein involved in the uptake of choline by cholinergic neurons. Defects in this gene can cause sialidosis, a lysosomal storage disease. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010].
Disorder MIM:	606107
Disorder Html:	?Deafness, autosomal dominant 72, 617606 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_025257.2	NP_079533.2	choline transporter-like protein 4 isoform 1
NM_025257.3	NP_079533.2	choline transporter-like protein 4 isoform 1
NM_001178044.1	NP_001171515.1	choline transporter-like protein 4 isoform 2
NM_001178045.1	NP_001171516.1	choline transporter-like protein 4 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC44A4	NP_079533.2
Anopheles gambiae (African malaria mosquito)	CTLH2_ANOGA	XP_311599.4
Bos taurus (cattle)	SLC44A4	NP_001076911.1
Danio rerio (zebrafish)	slc44a4	NP_956707.1
Drosophila melanogaster (fruit fly)	CG11880	NP_651670.1
Pan troglodytes (chimpanzee)	SLC44A4	XP_003339165.2
Canis lupus familiaris (dog)	SLC44A4	XP_538839.3
Macaca mulatta (Rhesus monkey)	SLC44A4	XP_002803721.1
Mus musculus (house mouse)	Slc44a4	NP_076046.1
Rattus norvegicus (Norway rat)	Slc44a4	NP_997706.1
Xenopus tropicalis (tropical clawed frog)	slc44a4	XP_002941312.2

Gene Ontology
Bibliography

Related articles in PubMed

Evaluating the Association of Common Variants of the SLC44A4 Gene with Ulcerative Colitis Susceptibility in the Han Chinese Population.
Wu J, Cheng Y, Zhang R, Shen H, Ma L, Yang J, Zhang Y, Zhang J
Genetic testing and molecular biomarkers21(9)555-559(2017 Sep)

SLC44A4 mutation causes autosomal dominant hereditary postlingual non-syndromic mid-frequency hearing loss.
Ma Z, Xia W, Liu F, Ma J, Sun S, Zhang J, Jiang N, Wang X, Hu J, Ma D
Human molecular genetics26(2)383-394(2017 01)

The human colonic thiamine pyrophosphate transporter (hTPPT) is a glycoprotein and N-linked glycosylation is important for its function.
Nabokina SM, Subramanian VS, Said HM
Biochimica et biophysica acta1858(4)866-71(2016 Apr)

A cross-ethnic survey of CFB and SLC44A4, Indian ulcerative colitis GWAS hits, underscores their potential role in disease susceptibility.
Gupta A, Juyal G, Sood A, Midha V, Yamazaki K, Vich Vila A, Esaki M, Matsui T, Takahashi A, Kubo M, Weersma RK, Thelma BK
European journal of human genetics : EJHG25(1)111-122(2016 01)

Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter.
Nabokina SM, Ramos MB, Said HM
PloS one11(2)e0149255(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC44A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC44A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19845	NM_001178044.1 Latest version!	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu19720	NM_025257.3 Latest version!	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00
OHu19793	NM_001178045.1 Latest version!	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$307.30 $439.00
View Old Accession Versions >>
OHu19720	NM_025257.2	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC44A4 ( NM_001178044.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171515.1 Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 2, mRNA.

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CloneID	OHu19845
Clone ID Related Accession (Same CDS sequence)	NM_001178044.1
Accession Version	NM_001178044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564675200000
Organism	Homo sapiens(human)
Product	choline transporter-like protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC300771.1, AK301596.1, AK300550.1, BC014659.1 and AI956094.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301596.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGGAA AGCAGCGGGA CGAGGATGAC GAGGCCTACG GGAAGCCAGT CAAATACGAC 
CCCTCCTTTC GAGGCCCCAT CAAGAACAGA AGCTGCACAG ATGTCATCTG CTGCGTCCTC 
TTCCTGCTCT TCATTCTAGG TTACATCGTG GTGGGGATTG TGGCCTGGTT GTATGGAGAC 
CCCCGGCAAG TCCTCTACCC CAGGAACTCT ACTGGGGCCT ACTGTGGCAT GGGGGAGAAC 
AAAGATAAGC CGTATCTCCT GTACTTCAAC ATCTTCAGCT GCATCCTGTC CAGCAACATC 
ATCTCAGTTG CTGAGAACGG CCTACAGTGC CCCACACCCC AGACGGTGAT CACAAGCCTG 
CAACAGGAAC TCTGCCCCAG TTTCCTCCTC CCCTCTGCTC CAGCTCTGGG GCGCTGCTTT 
CCATGGACCA ACGTTACTCC ACCGGCGCTC CCAGGGATCA CCAATGACAC CACCATACAG 
CAGGGGATCA GCGGTCTTAT TGACAGCCTC AATGCCCGAG ACATCAGTGT TAAGATCTTT 
GAAGATTTTG CCCAGTCCTG GTATTGGATT CTTGTTGCCC TGGGGGTGGC TCTGGTCTTG 
AGCCTACTGT TTATCTTGCT TCTGCGCCTG GTGGCTGGGC CCCTGGTGCT GGTGCTGATC 
CTGGGAGTGC TGGGCGTGCT GGCATACGGC ATCTACTACT GCTGGGAGGA GTACCGAGTG 
CTGCGGGACA AGGGCGCCTC CATCTCCCAG CTGGGTTTCA CCACCAACCT CAGTGCCTAC 
CAGAGCGTGC AGGAGACCTG GCTGGCCGCC CTGATCGTGT TGGCGGTGCT TGAAGCCATC 
CTGCTGCTGA TGCTCATCTT CCTGCGGCAG CGGATTCGTA TTGCCATCGC CCTCCTGAAG 
GAGGCCAGCA AGGCTGTGGG ACAGATGATG TCTACCATGT TCTACCCACT GGTCACCTTT 
GTCCTCCTCC TCATCTGCAT TGCCTACTGG GCCATGACTG CTCTGTACCT GGCTACATCG 
GGGCAACCCC AGTATGTGCT CTGGGCATCC AACATCAGCT CCCCCGGCTG TGAGAAAGTG 
CCAATAAATA CATCATGCAA CCCCACGGCC CACCTTGTGA ACTCCTCGTG CCCAGGGCTG 
ATGTGCGTCT TCCAGGGCTA CTCATCCAAA GGCCTAATCC AACGTTCTGT CTTCAATCTG 
CAAATCTATG GGGTCCTGGG GCTCTTCTGG ACCCTTAACT GGGTACTGGC CCTGGGCCAA 
TGCGTCCTCG CTGGAGCCTT TGCCTCCTTC TACTGGGCCT TCCACAAGCC CCAGGACATC 
CCTACCTTCC CCTTAATCTC TGCCTTCATC CGCACACTCC GTTACCACAC TGGGTCATTG 
GCATTTGGAG CCCTCATCCT GACCCTTGTG CAGATAGCCC GGGTCATCTT GGAGTATATT 
GACCACAAGC TCAGAGGAGT GCAGAACCCT GTAGCCCGCT GCATCATGTG CTGTTTCAAG 
TGCTGCCTCT GGTGTCTGGA AAAATTTATC AAGTTCCTAA ACCGCAATGC ATACATCATG 
ATCGCCATCT ACGGGAAGAA TTTCTGTGTC TCAGCCAAAA ATGCGTTCAT GCTACTCATG 
CGAAACATTG TCAGGGTGGT CGTCCTGGAC AAAGTCACAG ACCTGCTGCT GTTCTTTGGG 
AAGCTGCTGG TGGTCGGAGG CGTGGGGGTC CTGTCCTTCT TTTTTTTCTC CGGTCGCATC 
CCGGGGCTGG GTAAAGACTT TAAGAGCCCC CACCTCAACT ATTACTGGCT GCCCATCATG 
ACCTCCATCC TGGGGGCCTA TGTCATCGCC AGCGGCTTCT TCAGCGTTTT CGGCATGTGT 
GTGGACACGC TCTTCCTCTG CTTCCTGGAA GACCTGGAGC GGAACAACGG CTCCCTGGAC 
CGGCCCTACT ACATGTCCAA GAGCCTTCTA AAGATTCTGG GCAAGAAGAA CGAGGCGCCC 
CCGGACAACA AGAAGAGGAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171515.1
CDS	67..2073
Translation	MGGKQRDEDDEAYGKPVKYDPSFRGPIKNRSCTDVICCVLFLLFILGYIVVGIVAWLYGDPRQVLYPRNSTGAYCGMGENKDKPYLLYFNIFSCILSSNIISVAENGLQCPTPQTVITSLQQELCPSFLLPSAPALGRCFPWTNVTPPALPGITNDTTIQQGISGLIDSLNARDISVKIFEDFAQSWYWILVALGVALVLSLLFILLLRLVAGPLVLVLILGVLGVLAYGIYYCWEEYRVLRDKGASISQLGFTTNLSAYQSVQETWLAALIVLAVLEAILLLMLIFLRQRIRIAIALLKEASKAVGQMMSTMFYPLVTFVLLLICIAYWAMTALYLATSGQPQYVLWASNISSPGCEKVPINTSCNPTAHLVNSSCPGLMCVFQGYSSKGLIQRSVFNLQIYGVLGLFWTLNWVLALGQCVLAGAFASFYWAFHKPQDIPTFPLISAFIRTLRYHTGSLAFGALILTLVQIARVILEYIDHKLRGVQNPVARCIMCCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNIVRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIPGLGKDFKSPHLNYYWLPIMTSILGAYVIASGFFSVFGMCVDTLFLCFLEDLERNNGSLDRPYYMSKSLLKILGKKNEAPPDNKKRKK

Target ORF information:

RefSeq Version	NM_001178044.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178044.1

ORF Insert Sequence:

ATGGGGGGAA AGCAGCGGGA CGAGGATGAC GAGGCCTACG GGAAGCCAGT CAAATACGAC 
CCCTCCTTTC GAGGCCCCAT CAAGAACAGA AGCTGCACAG ATGTCATCTG CTGCGTCCTC 
TTCCTGCTCT TCATTCTAGG TTACATCGTG GTGGGGATTG TGGCCTGGTT GTATGGAGAC 
CCCCGGCAAG TCCTCTACCC CAGGAACTCT ACTGGGGCCT ACTGTGGCAT GGGGGAGAAC 
AAAGATAAGC CGTATCTCCT GTACTTCAAC ATCTTCAGCT GCATCCTGTC CAGCAACATC 
ATCTCAGTTG CTGAGAACGG CCTACAGTGC CCCACACCCC AGACGGTGAT CACAAGCCTG 
CAACAGGAAC TCTGCCCCAG TTTCCTCCTC CCCTCTGCTC CAGCTCTGGG GCGCTGCTTT 
CCATGGACCA ACGTTACTCC ACCGGCGCTC CCAGGGATCA CCAATGACAC CACCATACAG 
CAGGGGATCA GCGGTCTTAT TGACAGCCTC AATGCCCGAG ACATCAGTGT TAAGATCTTT 
GAAGATTTTG CCCAGTCCTG GTATTGGATT CTTGTTGCCC TGGGGGTGGC TCTGGTCTTG 
AGCCTACTGT TTATCTTGCT TCTGCGCCTG GTGGCTGGGC CCCTGGTGCT GGTGCTGATC 
CTGGGAGTGC TGGGCGTGCT GGCATACGGC ATCTACTACT GCTGGGAGGA GTACCGAGTG 
CTGCGGGACA AGGGCGCCTC CATCTCCCAG CTGGGTTTCA CCACCAACCT CAGTGCCTAC 
CAGAGCGTGC AGGAGACCTG GCTGGCCGCC CTGATCGTGT TGGCGGTGCT TGAAGCCATC 
CTGCTGCTGA TGCTCATCTT CCTGCGGCAG CGGATTCGTA TTGCCATCGC CCTCCTGAAG 
GAGGCCAGCA AGGCTGTGGG ACAGATGATG TCTACCATGT TCTACCCACT GGTCACCTTT 
GTCCTCCTCC TCATCTGCAT TGCCTACTGG GCCATGACTG CTCTGTACCT GGCTACATCG 
GGGCAACCCC AGTATGTGCT CTGGGCATCC AACATCAGCT CCCCCGGCTG TGAGAAAGTG 
CCAATAAATA CATCATGCAA CCCCACGGCC CACCTTGTGA ACTCCTCGTG CCCAGGGCTG 
ATGTGCGTCT TCCAGGGCTA CTCATCCAAA GGCCTAATCC AACGTTCTGT CTTCAATCTG 
CAAATCTATG GGGTCCTGGG GCTCTTCTGG ACCCTTAACT GGGTACTGGC CCTGGGCCAA 
TGCGTCCTCG CTGGAGCCTT TGCCTCCTTC TACTGGGCCT TCCACAAGCC CCAGGACATC 
CCTACCTTCC CCTTAATCTC TGCCTTCATC CGCACACTCC GTTACCACAC TGGGTCATTG 
GCATTTGGAG CCCTCATCCT GACCCTTGTG CAGATAGCCC GGGTCATCTT GGAGTATATT 
GACCACAAGC TCAGAGGAGT GCAGAACCCT GTAGCCCGCT GCATCATGTG CTGTTTCAAG 
TGCTGCCTCT GGTGTCTGGA AAAATTTATC AAGTTCCTAA ACCGCAATGC ATACATCATG 
ATCGCCATCT ACGGGAAGAA TTTCTGTGTC TCAGCCAAAA ATGCGTTCAT GCTACTCATG 
CGAAACATTG TCAGGGTGGT CGTCCTGGAC AAAGTCACAG ACCTGCTGCT GTTCTTTGGG 
AAGCTGCTGG TGGTCGGAGG CGTGGGGGTC CTGTCCTTCT TTTTTTTCTC CGGTCGCATC 
CCGGGGCTGG GTAAAGACTT TAAGAGCCCC CACCTCAACT ATTACTGGCT GCCCATCATG 
ACCTCCATCC TGGGGGCCTA TGTCATCGCC AGCGGCTTCT TCAGCGTTTT CGGCATGTGT 
GTGGACACGC TCTTCCTCTG CTTCCTGGAA GACCTGGAGC GGAACAACGG CTCCCTGGAC 
CGGCCCTACT ACATGTCCAA GAGCCTTCTA AAGATTCTGG GCAAGAAGAA CGAGGCGCCC 
CCGGACAACA AGAAGAGGAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC44A4 ( NM_025257.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_079533.2 Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19720
Clone ID Related Accession (Same CDS sequence)	NM_025257.2 , NM_025257.3
Accession Version	NM_025257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Homo sapiens(human)
Product	choline transporter-like protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC300771.1, BC014659.1, AK222998.1, DA924720.1 and AI560195.1. This sequence is a reference standard in the RefSeqGene project. On or before Jun 6, 2007 this sequence version replaced NM_032794.1, NM_025257.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014659.1, AK027397.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGGAA AGCAGCGGGA CGAGGATGAC GAGGCCTACG GGAAGCCAGT CAAATACGAC 
CCCTCCTTTC GAGGCCCCAT CAAGAACAGA AGCTGCACAG ATGTCATCTG CTGCGTCCTC 
TTCCTGCTCT TCATTCTAGG TTACATCGTG GTGGGGATTG TGGCCTGGTT GTATGGAGAC 
CCCCGGCAAG TCCTCTACCC CAGGAACTCT ACTGGGGCCT ACTGTGGCAT GGGGGAGAAC 
AAAGATAAGC CGTATCTCCT GTACTTCAAC ATCTTCAGCT GCATCCTGTC CAGCAACATC 
ATCTCAGTTG CTGAGAACGG CCTACAGTGC CCCACACCCC AGGTGTGTGT GTCCTCCTGC 
CCGGAGGACC CATGGACTGT GGGAAAAAAC GAGTTCTCAC AGACTGTTGG GGAAGTCTTC 
TATACAAAAA ACAGGAACTT TTGTCTGCCA GGGGTACCCT GGAATATGAC GGTGATCACA 
AGCCTGCAAC AGGAACTCTG CCCCAGTTTC CTCCTCCCCT CTGCTCCAGC TCTGGGGCGC 
TGCTTTCCAT GGACCAACGT TACTCCACCG GCGCTCCCAG GGATCACCAA TGACACCACC 
ATACAGCAGG GGATCAGCGG TCTTATTGAC AGCCTCAATG CCCGAGACAT CAGTGTTAAG 
ATCTTTGAAG ATTTTGCCCA GTCCTGGTAT TGGATTCTTG TTGCCCTGGG GGTGGCTCTG 
GTCTTGAGCC TACTGTTTAT CTTGCTTCTG CGCCTGGTGG CTGGGCCCCT GGTGCTGGTG 
CTGATCCTGG GAGTGCTGGG CGTGCTGGCA TACGGCATCT ACTACTGCTG GGAGGAGTAC 
CGAGTGCTGC GGGACAAGGG CGCCTCCATC TCCCAGCTGG GTTTCACCAC CAACCTCAGT 
GCCTACCAGA GCGTGCAGGA GACCTGGCTG GCCGCCCTGA TCGTGTTGGC GGTGCTTGAA 
GCCATCCTGC TGCTGATGCT CATCTTCCTG CGGCAGCGGA TTCGTATTGC CATCGCCCTC 
CTGAAGGAGG CCAGCAAGGC TGTGGGACAG ATGATGTCTA CCATGTTCTA CCCACTGGTC 
ACCTTTGTCC TCCTCCTCAT CTGCATTGCC TACTGGGCCA TGACTGCTCT GTACCTGGCT 
ACATCGGGGC AACCCCAGTA TGTGCTCTGG GCATCCAACA TCAGCTCCCC CGGCTGTGAG 
AAAGTGCCAA TAAATACATC ATGCAACCCC ACGGCCCACC TTGTGAACTC CTCGTGCCCA 
GGGCTGATGT GCGTCTTCCA GGGCTACTCA TCCAAAGGCC TAATCCAACG TTCTGTCTTC 
AATCTGCAAA TCTATGGGGT CCTGGGGCTC TTCTGGACCC TTAACTGGGT ACTGGCCCTG 
GGCCAATGCG TCCTCGCTGG AGCCTTTGCC TCCTTCTACT GGGCCTTCCA CAAGCCCCAG 
GACATCCCTA CCTTCCCCTT AATCTCTGCC TTCATCCGCA CACTCCGTTA CCACACTGGG 
TCATTGGCAT TTGGAGCCCT CATCCTGACC CTTGTGCAGA TAGCCCGGGT CATCTTGGAG 
TATATTGACC ACAAGCTCAG AGGAGTGCAG AACCCTGTAG CCCGCTGCAT CATGTGCTGT 
TTCAAGTGCT GCCTCTGGTG TCTGGAAAAA TTTATCAAGT TCCTAAACCG CAATGCATAC 
ATCATGATCG CCATCTACGG GAAGAATTTC TGTGTCTCAG CCAAAAATGC GTTCATGCTA 
CTCATGCGAA ACATTGTCAG GGTGGTCGTC CTGGACAAAG TCACAGACCT GCTGCTGTTC 
TTTGGGAAGC TGCTGGTGGT CGGAGGCGTG GGGGTCCTGT CCTTCTTTTT TTTCTCCGGT 
CGCATCCCGG GGCTGGGTAA AGACTTTAAG AGCCCCCACC TCAACTATTA CTGGCTGCCC 
ATCATGACCT CCATCCTGGG GGCCTATGTC ATCGCCAGCG GCTTCTTCAG CGTTTTCGGC 
ATGTGTGTGG ACACGCTCTT CCTCTGCTTC CTGGAAGACC TGGAGCGGAA CAACGGCTCC 
CTGGACCGGC CCTACTACAT GTCCAAGAGC CTTCTAAAGA TTCTGGGCAA GAAGAACGAG 
GCGCCCCCGG ACAACAAGAA GAGGAAGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079533.2
CDS	67..2199
Translation	MGGKQRDEDDEAYGKPVKYDPSFRGPIKNRSCTDVICCVLFLLFILGYIVVGIVAWLYGDPRQVLYPRNSTGAYCGMGENKDKPYLLYFNIFSCILSSNIISVAENGLQCPTPQVCVSSCPEDPWTVGKNEFSQTVGEVFYTKNRNFCLPGVPWNMTVITSLQQELCPSFLLPSAPALGRCFPWTNVTPPALPGITNDTTIQQGISGLIDSLNARDISVKIFEDFAQSWYWILVALGVALVLSLLFILLLRLVAGPLVLVLILGVLGVLAYGIYYCWEEYRVLRDKGASISQLGFTTNLSAYQSVQETWLAALIVLAVLEAILLLMLIFLRQRIRIAIALLKEASKAVGQMMSTMFYPLVTFVLLLICIAYWAMTALYLATSGQPQYVLWASNISSPGCEKVPINTSCNPTAHLVNSSCPGLMCVFQGYSSKGLIQRSVFNLQIYGVLGLFWTLNWVLALGQCVLAGAFASFYWAFHKPQDIPTFPLISAFIRTLRYHTGSLAFGALILTLVQIARVILEYIDHKLRGVQNPVARCIMCCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNIVRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIPGLGKDFKSPHLNYYWLPIMTSILGAYVIASGFFSVFGMCVDTLFLCFLEDLERNNGSLDRPYYMSKSLLKILGKKNEAPPDNKKRKK

Target ORF information:

RefSeq Version	NM_025257.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025257.2

ORF Insert Sequence:

ATGGGGGGAA AGCAGCGGGA CGAGGATGAC GAGGCCTACG GGAAGCCAGT CAAATACGAC 
CCCTCCTTTC GAGGCCCCAT CAAGAACAGA AGCTGCACAG ATGTCATCTG CTGCGTCCTC 
TTCCTGCTCT TCATTCTAGG TTACATCGTG GTGGGGATTG TGGCCTGGTT GTATGGAGAC 
CCCCGGCAAG TCCTCTACCC CAGGAACTCT ACTGGGGCCT ACTGTGGCAT GGGGGAGAAC 
AAAGATAAGC CGTATCTCCT GTACTTCAAC ATCTTCAGCT GCATCCTGTC CAGCAACATC 
ATCTCAGTTG CTGAGAACGG CCTACAGTGC CCCACACCCC AGGTGTGTGT GTCCTCCTGC 
CCGGAGGACC CATGGACTGT GGGAAAAAAC GAGTTCTCAC AGACTGTTGG GGAAGTCTTC 
TATACAAAAA ACAGGAACTT TTGTCTGCCA GGGGTACCCT GGAATATGAC GGTGATCACA 
AGCCTGCAAC AGGAACTCTG CCCCAGTTTC CTCCTCCCCT CTGCTCCAGC TCTGGGGCGC 
TGCTTTCCAT GGACCAACGT TACTCCACCG GCGCTCCCAG GGATCACCAA TGACACCACC 
ATACAGCAGG GGATCAGCGG TCTTATTGAC AGCCTCAATG CCCGAGACAT CAGTGTTAAG 
ATCTTTGAAG ATTTTGCCCA GTCCTGGTAT TGGATTCTTG TTGCCCTGGG GGTGGCTCTG 
GTCTTGAGCC TACTGTTTAT CTTGCTTCTG CGCCTGGTGG CTGGGCCCCT GGTGCTGGTG 
CTGATCCTGG GAGTGCTGGG CGTGCTGGCA TACGGCATCT ACTACTGCTG GGAGGAGTAC 
CGAGTGCTGC GGGACAAGGG CGCCTCCATC TCCCAGCTGG GTTTCACCAC CAACCTCAGT 
GCCTACCAGA GCGTGCAGGA GACCTGGCTG GCCGCCCTGA TCGTGTTGGC GGTGCTTGAA 
GCCATCCTGC TGCTGATGCT CATCTTCCTG CGGCAGCGGA TTCGTATTGC CATCGCCCTC 
CTGAAGGAGG CCAGCAAGGC TGTGGGACAG ATGATGTCTA CCATGTTCTA CCCACTGGTC 
ACCTTTGTCC TCCTCCTCAT CTGCATTGCC TACTGGGCCA TGACTGCTCT GTACCTGGCT 
ACATCGGGGC AACCCCAGTA TGTGCTCTGG GCATCCAACA TCAGCTCCCC CGGCTGTGAG 
AAAGTGCCAA TAAATACATC ATGCAACCCC ACGGCCCACC TTGTGAACTC CTCGTGCCCA 
GGGCTGATGT GCGTCTTCCA GGGCTACTCA TCCAAAGGCC TAATCCAACG TTCTGTCTTC 
AATCTGCAAA TCTATGGGGT CCTGGGGCTC TTCTGGACCC TTAACTGGGT ACTGGCCCTG 
GGCCAATGCG TCCTCGCTGG AGCCTTTGCC TCCTTCTACT GGGCCTTCCA CAAGCCCCAG 
GACATCCCTA CCTTCCCCTT AATCTCTGCC TTCATCCGCA CACTCCGTTA CCACACTGGG 
TCATTGGCAT TTGGAGCCCT CATCCTGACC CTTGTGCAGA TAGCCCGGGT CATCTTGGAG 
TATATTGACC ACAAGCTCAG AGGAGTGCAG AACCCTGTAG CCCGCTGCAT CATGTGCTGT 
TTCAAGTGCT GCCTCTGGTG TCTGGAAAAA TTTATCAAGT TCCTAAACCG CAATGCATAC 
ATCATGATCG CCATCTACGG GAAGAATTTC TGTGTCTCAG CCAAAAATGC GTTCATGCTA 
CTCATGCGAA ACATTGTCAG GGTGGTCGTC CTGGACAAAG TCACAGACCT GCTGCTGTTC 
TTTGGGAAGC TGCTGGTGGT CGGAGGCGTG GGGGTCCTGT CCTTCTTTTT TTTCTCCGGT 
CGCATCCCGG GGCTGGGTAA AGACTTTAAG AGCCCCCACC TCAACTATTA CTGGCTGCCC 
ATCATGACCT CCATCCTGGG GGCCTATGTC ATCGCCAGCG GCTTCTTCAG CGTTTTCGGC 
ATGTGTGTGG ACACGCTCTT CCTCTGCTTC CTGGAAGACC TGGAGCGGAA CAACGGCTCC 
CTGGACCGGC CCTACTACAT GTCCAAGAGC CTTCTAAAGA TTCTGGGCAA GAAGAACGAG 
GCGCCCCCGG ACAACAAGAA GAGGAAGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC44A4 ( NM_025257.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079533.2 Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 1, mRNA.

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CloneID	OHu19720
Clone ID Related Accession (Same CDS sequence)	NM_025257.2 , NM_025257.3
Accession Version	NM_025257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1566316800000
Organism	Homo sapiens(human)
Product	choline transporter-like protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC300771.1, BC014659.1, AK222998.1, DA924720.1 and AI560195.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_025257.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK027397.1, BC014659.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000229729.11/ ENSP00000229729.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGGGAA AGCAGCGGGA CGAGGATGAC GAGGCCTACG GGAAGCCAGT CAAATACGAC 
CCCTCCTTTC GAGGCCCCAT CAAGAACAGA AGCTGCACAG ATGTCATCTG CTGCGTCCTC 
TTCCTGCTCT TCATTCTAGG TTACATCGTG GTGGGGATTG TGGCCTGGTT GTATGGAGAC 
CCCCGGCAAG TCCTCTACCC CAGGAACTCT ACTGGGGCCT ACTGTGGCAT GGGGGAGAAC 
AAAGATAAGC CGTATCTCCT GTACTTCAAC ATCTTCAGCT GCATCCTGTC CAGCAACATC 
ATCTCAGTTG CTGAGAACGG CCTACAGTGC CCCACACCCC AGGTGTGTGT GTCCTCCTGC 
CCGGAGGACC CATGGACTGT GGGAAAAAAC GAGTTCTCAC AGACTGTTGG GGAAGTCTTC 
TATACAAAAA ACAGGAACTT TTGTCTGCCA GGGGTACCCT GGAATATGAC GGTGATCACA 
AGCCTGCAAC AGGAACTCTG CCCCAGTTTC CTCCTCCCCT CTGCTCCAGC TCTGGGGCGC 
TGCTTTCCAT GGACCAACGT TACTCCACCG GCGCTCCCAG GGATCACCAA TGACACCACC 
ATACAGCAGG GGATCAGCGG TCTTATTGAC AGCCTCAATG CCCGAGACAT CAGTGTTAAG 
ATCTTTGAAG ATTTTGCCCA GTCCTGGTAT TGGATTCTTG TTGCCCTGGG GGTGGCTCTG 
GTCTTGAGCC TACTGTTTAT CTTGCTTCTG CGCCTGGTGG CTGGGCCCCT GGTGCTGGTG 
CTGATCCTGG GAGTGCTGGG CGTGCTGGCA TACGGCATCT ACTACTGCTG GGAGGAGTAC 
CGAGTGCTGC GGGACAAGGG CGCCTCCATC TCCCAGCTGG GTTTCACCAC CAACCTCAGT 
GCCTACCAGA GCGTGCAGGA GACCTGGCTG GCCGCCCTGA TCGTGTTGGC GGTGCTTGAA 
GCCATCCTGC TGCTGATGCT CATCTTCCTG CGGCAGCGGA TTCGTATTGC CATCGCCCTC 
CTGAAGGAGG CCAGCAAGGC TGTGGGACAG ATGATGTCTA CCATGTTCTA CCCACTGGTC 
ACCTTTGTCC TCCTCCTCAT CTGCATTGCC TACTGGGCCA TGACTGCTCT GTACCTGGCT 
ACATCGGGGC AACCCCAGTA TGTGCTCTGG GCATCCAACA TCAGCTCCCC CGGCTGTGAG 
AAAGTGCCAA TAAATACATC ATGCAACCCC ACGGCCCACC TTGTGAACTC CTCGTGCCCA 
GGGCTGATGT GCGTCTTCCA GGGCTACTCA TCCAAAGGCC TAATCCAACG TTCTGTCTTC 
AATCTGCAAA TCTATGGGGT CCTGGGGCTC TTCTGGACCC TTAACTGGGT ACTGGCCCTG 
GGCCAATGCG TCCTCGCTGG AGCCTTTGCC TCCTTCTACT GGGCCTTCCA CAAGCCCCAG 
GACATCCCTA CCTTCCCCTT AATCTCTGCC TTCATCCGCA CACTCCGTTA CCACACTGGG 
TCATTGGCAT TTGGAGCCCT CATCCTGACC CTTGTGCAGA TAGCCCGGGT CATCTTGGAG 
TATATTGACC ACAAGCTCAG AGGAGTGCAG AACCCTGTAG CCCGCTGCAT CATGTGCTGT 
TTCAAGTGCT GCCTCTGGTG TCTGGAAAAA TTTATCAAGT TCCTAAACCG CAATGCATAC 
ATCATGATCG CCATCTACGG GAAGAATTTC TGTGTCTCAG CCAAAAATGC GTTCATGCTA 
CTCATGCGAA ACATTGTCAG GGTGGTCGTC CTGGACAAAG TCACAGACCT GCTGCTGTTC 
TTTGGGAAGC TGCTGGTGGT CGGAGGCGTG GGGGTCCTGT CCTTCTTTTT TTTCTCCGGT 
CGCATCCCGG GGCTGGGTAA AGACTTTAAG AGCCCCCACC TCAACTATTA CTGGCTGCCC 
ATCATGACCT CCATCCTGGG GGCCTATGTC ATCGCCAGCG GCTTCTTCAG CGTTTTCGGC 
ATGTGTGTGG ACACGCTCTT CCTCTGCTTC CTGGAAGACC TGGAGCGGAA CAACGGCTCC 
CTGGACCGGC CCTACTACAT GTCCAAGAGC CTTCTAAAGA TTCTGGGCAA GAAGAACGAG 
GCGCCCCCGG ACAACAAGAA GAGGAAGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079533.2
CDS	18..2150
Translation	MGGKQRDEDDEAYGKPVKYDPSFRGPIKNRSCTDVICCVLFLLFILGYIVVGIVAWLYGDPRQVLYPRNSTGAYCGMGENKDKPYLLYFNIFSCILSSNIISVAENGLQCPTPQVCVSSCPEDPWTVGKNEFSQTVGEVFYTKNRNFCLPGVPWNMTVITSLQQELCPSFLLPSAPALGRCFPWTNVTPPALPGITNDTTIQQGISGLIDSLNARDISVKIFEDFAQSWYWILVALGVALVLSLLFILLLRLVAGPLVLVLILGVLGVLAYGIYYCWEEYRVLRDKGASISQLGFTTNLSAYQSVQETWLAALIVLAVLEAILLLMLIFLRQRIRIAIALLKEASKAVGQMMSTMFYPLVTFVLLLICIAYWAMTALYLATSGQPQYVLWASNISSPGCEKVPINTSCNPTAHLVNSSCPGLMCVFQGYSSKGLIQRSVFNLQIYGVLGLFWTLNWVLALGQCVLAGAFASFYWAFHKPQDIPTFPLISAFIRTLRYHTGSLAFGALILTLVQIARVILEYIDHKLRGVQNPVARCIMCCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNIVRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIPGLGKDFKSPHLNYYWLPIMTSILGAYVIASGFFSVFGMCVDTLFLCFLEDLERNNGSLDRPYYMSKSLLKILGKKNEAPPDNKKRKK

Target ORF information:

RefSeq Version	NM_025257.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025257.3

ORF Insert Sequence:

ATGGGGGGAA AGCAGCGGGA CGAGGATGAC GAGGCCTACG GGAAGCCAGT CAAATACGAC 
CCCTCCTTTC GAGGCCCCAT CAAGAACAGA AGCTGCACAG ATGTCATCTG CTGCGTCCTC 
TTCCTGCTCT TCATTCTAGG TTACATCGTG GTGGGGATTG TGGCCTGGTT GTATGGAGAC 
CCCCGGCAAG TCCTCTACCC CAGGAACTCT ACTGGGGCCT ACTGTGGCAT GGGGGAGAAC 
AAAGATAAGC CGTATCTCCT GTACTTCAAC ATCTTCAGCT GCATCCTGTC CAGCAACATC 
ATCTCAGTTG CTGAGAACGG CCTACAGTGC CCCACACCCC AGGTGTGTGT GTCCTCCTGC 
CCGGAGGACC CATGGACTGT GGGAAAAAAC GAGTTCTCAC AGACTGTTGG GGAAGTCTTC 
TATACAAAAA ACAGGAACTT TTGTCTGCCA GGGGTACCCT GGAATATGAC GGTGATCACA 
AGCCTGCAAC AGGAACTCTG CCCCAGTTTC CTCCTCCCCT CTGCTCCAGC TCTGGGGCGC 
TGCTTTCCAT GGACCAACGT TACTCCACCG GCGCTCCCAG GGATCACCAA TGACACCACC 
ATACAGCAGG GGATCAGCGG TCTTATTGAC AGCCTCAATG CCCGAGACAT CAGTGTTAAG 
ATCTTTGAAG ATTTTGCCCA GTCCTGGTAT TGGATTCTTG TTGCCCTGGG GGTGGCTCTG 
GTCTTGAGCC TACTGTTTAT CTTGCTTCTG CGCCTGGTGG CTGGGCCCCT GGTGCTGGTG 
CTGATCCTGG GAGTGCTGGG CGTGCTGGCA TACGGCATCT ACTACTGCTG GGAGGAGTAC 
CGAGTGCTGC GGGACAAGGG CGCCTCCATC TCCCAGCTGG GTTTCACCAC CAACCTCAGT 
GCCTACCAGA GCGTGCAGGA GACCTGGCTG GCCGCCCTGA TCGTGTTGGC GGTGCTTGAA 
GCCATCCTGC TGCTGATGCT CATCTTCCTG CGGCAGCGGA TTCGTATTGC CATCGCCCTC 
CTGAAGGAGG CCAGCAAGGC TGTGGGACAG ATGATGTCTA CCATGTTCTA CCCACTGGTC 
ACCTTTGTCC TCCTCCTCAT CTGCATTGCC TACTGGGCCA TGACTGCTCT GTACCTGGCT 
ACATCGGGGC AACCCCAGTA TGTGCTCTGG GCATCCAACA TCAGCTCCCC CGGCTGTGAG 
AAAGTGCCAA TAAATACATC ATGCAACCCC ACGGCCCACC TTGTGAACTC CTCGTGCCCA 
GGGCTGATGT GCGTCTTCCA GGGCTACTCA TCCAAAGGCC TAATCCAACG TTCTGTCTTC 
AATCTGCAAA TCTATGGGGT CCTGGGGCTC TTCTGGACCC TTAACTGGGT ACTGGCCCTG 
GGCCAATGCG TCCTCGCTGG AGCCTTTGCC TCCTTCTACT GGGCCTTCCA CAAGCCCCAG 
GACATCCCTA CCTTCCCCTT AATCTCTGCC TTCATCCGCA CACTCCGTTA CCACACTGGG 
TCATTGGCAT TTGGAGCCCT CATCCTGACC CTTGTGCAGA TAGCCCGGGT CATCTTGGAG 
TATATTGACC ACAAGCTCAG AGGAGTGCAG AACCCTGTAG CCCGCTGCAT CATGTGCTGT 
TTCAAGTGCT GCCTCTGGTG TCTGGAAAAA TTTATCAAGT TCCTAAACCG CAATGCATAC 
ATCATGATCG CCATCTACGG GAAGAATTTC TGTGTCTCAG CCAAAAATGC GTTCATGCTA 
CTCATGCGAA ACATTGTCAG GGTGGTCGTC CTGGACAAAG TCACAGACCT GCTGCTGTTC 
TTTGGGAAGC TGCTGGTGGT CGGAGGCGTG GGGGTCCTGT CCTTCTTTTT TTTCTCCGGT 
CGCATCCCGG GGCTGGGTAA AGACTTTAAG AGCCCCCACC TCAACTATTA CTGGCTGCCC 
ATCATGACCT CCATCCTGGG GGCCTATGTC ATCGCCAGCG GCTTCTTCAG CGTTTTCGGC 
ATGTGTGTGG ACACGCTCTT CCTCTGCTTC CTGGAAGACC TGGAGCGGAA CAACGGCTCC 
CTGGACCGGC CCTACTACAT GTCCAAGAGC CTTCTAAAGA TTCTGGGCAA GAAGAACGAG 
GCGCCCCCGG ACAACAAGAA GAGGAAGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC44A4 ( NM_001178045.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171516.1 Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 3, mRNA.

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CloneID	OHu19793
Clone ID Related Accession (Same CDS sequence)	NM_001178045.1
Accession Version	NM_001178045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564675200000
Organism	Homo sapiens(human)
Product	choline transporter-like protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300550.1, DC392935.1, BC014659.1 and AI956094.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300550.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA ACAAAGATAA GCCGTATCTC CTGTACTTCA ACATCTTCAG CTGCATCCTG 
TCCAGCAACA TCATCTCAGT TGCTGAGAAC GGCCTACAGT GCCCCACACC CCAGGTGTGT 
GTGTCCTCCT GCCCGGAGGA CCCATGGACT GTGGGAAAAA ACGAGTTCTC ACAGACTGTT 
GGGGAAGTCT TCTATACAAA AAACAGGAAC TTTTGTCTGC CAGGGGTACC CTGGAATATG 
ACGGTGATCA CAAGCCTGCA ACAGGAACTC TGCCCCAGTT TCCTCCTCCC CTCTGCTCCA 
GCTCTGGGGC GCTGCTTTCC ATGGACCAAC GTTACTCCAC CGGCGCTCCC AGGGATCACC 
AATGACACCA CCATACAGCA GGGGATCAGC GGTCTTATTG ACAGCCTCAA TGCCCGAGAC 
ATCAGTGTTA AGATCTTTGA AGATTTTGCC CAGTCCTGGT ATTGGATTCT TGTTGCCCTG 
GGGGTGGCTC TGGTCTTGAG CCTACTGTTT ATCTTGCTTC TGCGCCTGGT GGCTGGGCCC 
CTGGTGCTGG TGCTGATCCT GGGAGTGCTG GGCGTGCTGG CATACGGCAT CTACTACTGC 
TGGGAGGAGT ACCGAGTGCT GCGGGACAAG GGCGCCTCCA TCTCCCAGCT GGGTTTCACC 
ACCAACCTCA GTGCCTACCA GAGCGTGCAG GAGACCTGGC TGGCCGCCCT GATCGTGTTG 
GCGGTGCTTG AAGCCATCCT GCTGCTGATG CTCATCTTCC TGCGGCAGCG GATTCGTATT 
GCCATCGCCC TCCTGAAGGA GGCCAGCAAG GCTGTGGGAC AGATGATGTC TACCATGTTC 
TACCCACTGG TCACCTTTGT CCTCCTCCTC ATCTGCATTG CCTACTGGGC CATGACTGCT 
CTGTACCTGG CTACATCGGG GCAACCCCAG TATGTGCTCT GGGCATCCAA CATCAGCTCC 
CCCGGCTGTG AGAAAGTGCC AATAAATACA TCATGCAACC CCACGGCCCA CCTTGTGAAC 
TCCTCGTGCC CAGGGCTGAT GTGCGTCTTC CAGGGCTACT CATCCAAAGG CCTAATCCAA 
CGTTCTGTCT TCAATCTGCA AATCTATGGG GTCCTGGGGC TCTTCTGGAC CCTTAACTGG 
GTACTGGCCC TGGGCCAATG CGTCCTCGCT GGAGCCTTTG CCTCCTTCTA CTGGGCCTTC 
CACAAGCCCC AGGACATCCC TACCTTCCCC TTAATCTCTG CCTTCATCCG CACACTCCGT 
TACCACACTG GGTCATTGGC ATTTGGAGCC CTCATCCTGA CCCTTGTGCA GATAGCCCGG 
GTCATCTTGG AGTATATTGA CCACAAGCTC AGAGGAGTGC AGAACCCTGT AGCCCGCTGC 
ATCATGTGCT GTTTCAAGTG CTGCCTCTGG TGTCTGGAAA AATTTATCAA GTTCCTAAAC 
CGCAATGCAT ACATCATGAT CGCCATCTAC GGGAAGAATT TCTGTGTCTC AGCCAAAAAT 
GCGTTCATGC TACTCATGCG AAACATTGTC AGGGTGGTCG TCCTGGACAA AGTCACAGAC 
CTGCTGCTGT TCTTTGGGAA GCTGCTGGTG GTCGGAGGCG TGGGGGTCCT GTCCTTCTTT 
TTTTTCTCCG GTCGCATCCC GGGGCTGGGT AAAGACTTTA AGAGCCCCCA CCTCAACTAT 
TACTGGCTGC CCATCATGAC CTCCATCCTG GGGGCCTATG TCATCGCCAG CGGCTTCTTC 
AGCGTTTTCG GCATGTGTGT GGACACGCTC TTCCTCTGCT TCCTGGAAGA CCTGGAGCGG 
AACAACGGCT CCCTGGACCG GCCCTACTAC ATGTCCAAGA GCCTTCTAAA GATTCTGGGC 
AAGAAGAACG AGGCGCCCCC GGACAACAAG AAGAGGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171516.1
CDS	298..2202
Translation	MGENKDKPYLLYFNIFSCILSSNIISVAENGLQCPTPQVCVSSCPEDPWTVGKNEFSQTVGEVFYTKNRNFCLPGVPWNMTVITSLQQELCPSFLLPSAPALGRCFPWTNVTPPALPGITNDTTIQQGISGLIDSLNARDISVKIFEDFAQSWYWILVALGVALVLSLLFILLLRLVAGPLVLVLILGVLGVLAYGIYYCWEEYRVLRDKGASISQLGFTTNLSAYQSVQETWLAALIVLAVLEAILLLMLIFLRQRIRIAIALLKEASKAVGQMMSTMFYPLVTFVLLLICIAYWAMTALYLATSGQPQYVLWASNISSPGCEKVPINTSCNPTAHLVNSSCPGLMCVFQGYSSKGLIQRSVFNLQIYGVLGLFWTLNWVLALGQCVLAGAFASFYWAFHKPQDIPTFPLISAFIRTLRYHTGSLAFGALILTLVQIARVILEYIDHKLRGVQNPVARCIMCCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNIVRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIPGLGKDFKSPHLNYYWLPIMTSILGAYVIASGFFSVFGMCVDTLFLCFLEDLERNNGSLDRPYYMSKSLLKILGKKNEAPPDNKKRKK

Target ORF information:

RefSeq Version	NM_001178045.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 44 member 4 (SLC44A4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178045.1

ORF Insert Sequence:

ATGGGGGAGA ACAAAGATAA GCCGTATCTC CTGTACTTCA ACATCTTCAG CTGCATCCTG 
TCCAGCAACA TCATCTCAGT TGCTGAGAAC GGCCTACAGT GCCCCACACC CCAGGTGTGT 
GTGTCCTCCT GCCCGGAGGA CCCATGGACT GTGGGAAAAA ACGAGTTCTC ACAGACTGTT 
GGGGAAGTCT TCTATACAAA AAACAGGAAC TTTTGTCTGC CAGGGGTACC CTGGAATATG 
ACGGTGATCA CAAGCCTGCA ACAGGAACTC TGCCCCAGTT TCCTCCTCCC CTCTGCTCCA 
GCTCTGGGGC GCTGCTTTCC ATGGACCAAC GTTACTCCAC CGGCGCTCCC AGGGATCACC 
AATGACACCA CCATACAGCA GGGGATCAGC GGTCTTATTG ACAGCCTCAA TGCCCGAGAC 
ATCAGTGTTA AGATCTTTGA AGATTTTGCC CAGTCCTGGT ATTGGATTCT TGTTGCCCTG 
GGGGTGGCTC TGGTCTTGAG CCTACTGTTT ATCTTGCTTC TGCGCCTGGT GGCTGGGCCC 
CTGGTGCTGG TGCTGATCCT GGGAGTGCTG GGCGTGCTGG CATACGGCAT CTACTACTGC 
TGGGAGGAGT ACCGAGTGCT GCGGGACAAG GGCGCCTCCA TCTCCCAGCT GGGTTTCACC 
ACCAACCTCA GTGCCTACCA GAGCGTGCAG GAGACCTGGC TGGCCGCCCT GATCGTGTTG 
GCGGTGCTTG AAGCCATCCT GCTGCTGATG CTCATCTTCC TGCGGCAGCG GATTCGTATT 
GCCATCGCCC TCCTGAAGGA GGCCAGCAAG GCTGTGGGAC AGATGATGTC TACCATGTTC 
TACCCACTGG TCACCTTTGT CCTCCTCCTC ATCTGCATTG CCTACTGGGC CATGACTGCT 
CTGTACCTGG CTACATCGGG GCAACCCCAG TATGTGCTCT GGGCATCCAA CATCAGCTCC 
CCCGGCTGTG AGAAAGTGCC AATAAATACA TCATGCAACC CCACGGCCCA CCTTGTGAAC 
TCCTCGTGCC CAGGGCTGAT GTGCGTCTTC CAGGGCTACT CATCCAAAGG CCTAATCCAA 
CGTTCTGTCT TCAATCTGCA AATCTATGGG GTCCTGGGGC TCTTCTGGAC CCTTAACTGG 
GTACTGGCCC TGGGCCAATG CGTCCTCGCT GGAGCCTTTG CCTCCTTCTA CTGGGCCTTC 
CACAAGCCCC AGGACATCCC TACCTTCCCC TTAATCTCTG CCTTCATCCG CACACTCCGT 
TACCACACTG GGTCATTGGC ATTTGGAGCC CTCATCCTGA CCCTTGTGCA GATAGCCCGG 
GTCATCTTGG AGTATATTGA CCACAAGCTC AGAGGAGTGC AGAACCCTGT AGCCCGCTGC 
ATCATGTGCT GTTTCAAGTG CTGCCTCTGG TGTCTGGAAA AATTTATCAA GTTCCTAAAC 
CGCAATGCAT ACATCATGAT CGCCATCTAC GGGAAGAATT TCTGTGTCTC AGCCAAAAAT 
GCGTTCATGC TACTCATGCG AAACATTGTC AGGGTGGTCG TCCTGGACAA AGTCACAGAC 
CTGCTGCTGT TCTTTGGGAA GCTGCTGGTG GTCGGAGGCG TGGGGGTCCT GTCCTTCTTT 
TTTTTCTCCG GTCGCATCCC GGGGCTGGGT AAAGACTTTA AGAGCCCCCA CCTCAACTAT 
TACTGGCTGC CCATCATGAC CTCCATCCTG GGGGCCTATG TCATCGCCAG CGGCTTCTTC 
AGCGTTTTCG GCATGTGTGT GGACACGCTC TTCCTCTGCT TCCTGGAAGA CCTGGAGCGG 
AACAACGGCT CCCTGGACCG GCCCTACTAC ATGTCCAAGA GCCTTCTAAA GATTCTGGGC 
AAGAAGAACG AGGCGCCCCC GGACAACAAG AAGAGGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluating the Association of Common Variants of the SLC44A4 Gene with Ulcerative Colitis Susceptibility in the Han Chinese Population.
Genetic testing and molecular biomarkers21(9)555-559(2017 Sep)
Wu J,Cheng Y,Zhang R,Shen H,Ma L,Yang J,Zhang Y,Zhang J

SLC44A4 mutation causes autosomal dominant hereditary postlingual non-syndromic mid-frequency hearing loss.
Human molecular genetics26(2)383-394(2017 01)
Ma Z,Xia W,Liu F,Ma J,Sun S,Zhang J,Jiang N,Wang X,Hu J,Ma D

The human colonic thiamine pyrophosphate transporter (hTPPT) is a glycoprotein and N-linked glycosylation is important for its function.
Biochimica et biophysica acta1858(4)866-71(2016 Apr)
Nabokina SM,Subramanian VS,Said HM

A cross-ethnic survey of CFB and SLC44A4, Indian ulcerative colitis GWAS hits, underscores their potential role in disease susceptibility.
European journal of human genetics : EJHG25(1)111-122(2016 01)
Gupta A,Juyal G,Sood A,Midha V,Yamazaki K,Vich Vila A,Esaki M,Matsui T,Takahashi A,Kubo M,Weersma RK,Thelma BK

Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter.
PloS one11(2)e0149255(2016)
Nabokina SM,Ramos MB,Said HM

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SLC44A4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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