| Gene Symbol | NAA50 |
| Entrez Gene ID | 80218 |
| Full Name | N(alpha)-acetyltransferase 50, NatE catalytic subunit |
| Synonyms | MAK3,NAT13,NAT13P,NAT5,NAT5P,SAN,hNaa50p |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Disorder MIM: |
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NAA50 cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following NAA50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAA50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu38158 Clone ID Related Accession (Same CDS sequence) NM_001308445.1 , NM_001308445.2 Accession Version NM_001308445.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 507bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1550937600000 Organism Homo sapiens(human) Product N-alpha-acetyltransferase 50 isoform 2 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108693.5, AL601126.1 and BX102492.1. On May 5, 2015 this sequence version replaced XM_005247794.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.51106.1, SRR1660803.1249.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT
GCAGATGTGC AAAAGACAGA CAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001295374.1 CDS 351..857 Translation 
Target ORF information:
RefSeq Version NM_001308445.1 Organism Homo sapiens(human) Definition Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_001308445.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT
GCAGATGTGC AAAAGACAGA CAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu38158 Clone ID Related Accession (Same CDS sequence) NM_001308445.1 , NM_001308445.2 Accession Version NM_001308445.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 507bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1574697600000 Organism Homo sapiens(human) Product N-alpha-acetyltransferase 50 isoform 2 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108693.5, AL601126.1 and BX102492.1. On May 31, 2019 this sequence version replaced NM_001308445.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.20858.1, SRR1803614.121389.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT
GCAGATGTGC AAAAGACAGA CAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001295374.1 CDS 301..807 Translation
Target ORF information:
RefSeq Version NM_001308445.2 Organism Homo sapiens(human) Definition Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001308445.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481ATGAAAGGCC GGATCGAGCT GGGAGATGTG ACACCACACA ATATTAAACA GTTGAAAAGA
TTGAATCAGG TCATCTTTCC AGTCAGCTAC AATGACAAGT TCTACAAGGA TGTGCTGGAG
GTTGGCGAGC TAGCAAAACT TGCCTATTTC AATGATATTG CTGTAGGTGC AGTATGCTGT
AGGGTGGATC ATTCACAGAA TCAGAAGAGA CTTTACATCA TGACACTAGG ATGTCTGGCA
CCTTACCGAA GGCTAGGAAT AGGAACTAAA ATGTTAAATC ATGTCTTAAA CATCTGTGAA
AAAGATGGTA CTTTTGACAA CATTTATCTG CATGTCCAGA TCAGCAATGA GTCGGCAATT
GACTTCTACA GGAAGTTTGG CTTTGAGATT ATTGAGACAA AGAAGAACTA CTATAAGAGG
ATAGAGCCCG CAGATGCTCA TGTGCTGCAG AAAAACCTCA AAGTTCCTTC TGGTCAGAAT
GCAGATGTGC AAAAGACAGA CAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu15478 Clone ID Related Accession (Same CDS sequence) NM_025146.4 , NM_025146.3 Accession Version NM_025146.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 510bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1574697600000 Organism Homo sapiens(human) Product N-alpha-acetyltransferase 50 isoform 1 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM831426.1, BC012731.2, DB198553.1, DB096143.1, DA408894.1, DA599265.1, HX784836.1, AW954963.1, H15889.1, H39948.1, BX102492.1 and AC108693.5. On Nov 22, 2018 this sequence version replaced NM_025146.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK023256.1, SRR1803612.19842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000240922.8/ ENSP00000240922.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.
1
61
121
181
241
301
361
421
481ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG
AATGCAGATG TGCAAAAGAC AGACAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_079422.1 CDS 301..810 Translation
Target ORF information:
RefSeq Version NM_025146.4 Organism Homo sapiens(human) Definition Homo sapiens N-alpha-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_025146.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG
AATGCAGATG TGCAAAAGAC AGACAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu15478 Clone ID Related Accession (Same CDS sequence) NM_025146.4 , NM_025146.3 Accession Version NM_025146.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 510bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1506873600000 Organism Homo sapiens(human) Product N-alpha-acetyltransferase 50 isoform 1 Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA854531.1, BM831426.1, BC012731.2, DB198553.1, DB096143.1, DA408894.1, DA599265.1, HX784836.1, AW954963.1, H15889.1, H39948.1 and BX102492.1. On May 5, 2015 this sequence version replaced NM_025146.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.19842.1, SRR1803615.249830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG
AATGCAGATG TGCAAAAGAC AGACAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_079422.1 CDS 351..860 Translation
Target ORF information:
RefSeq Version NM_025146.3 Organism Homo sapiens(human) Definition Homo sapiens N(alpha)-acetyltransferase 50, NatE catalytic subunit (NAA50), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_025146.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481ATGAAAGGTA GCCGGATCGA GCTGGGAGAT GTGACACCAC ACAATATTAA ACAGTTGAAA
AGATTGAATC AGGTCATCTT TCCAGTCAGC TACAATGACA AGTTCTACAA GGATGTGCTG
GAGGTTGGCG AGCTAGCAAA ACTTGCCTAT TTCAATGATA TTGCTGTAGG TGCAGTATGC
TGTAGGGTGG ATCATTCACA GAATCAGAAG AGACTTTACA TCATGACACT AGGATGTCTG
GCACCTTACC GAAGGCTAGG AATAGGAACT AAAATGTTAA ATCATGTCTT AAACATCTGT
GAAAAAGATG GTACTTTTGA CAACATTTAT CTGCATGTCC AGATCAGCAA TGAGTCGGCA
ATTGACTTCT ACAGGAAGTT TGGCTTTGAG ATTATTGAGA CAAAGAAGAA CTACTATAAG
AGGATAGAGC CCGCAGATGC TCATGTGCTG CAGAAAAACC TCAAAGTTCC TTCTGGTCAG
AATGCAGATG TGCAAAAGAC AGACAACTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
-
PubMed
Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW
Human Naa50 Protein Displays Broad Substrate Specificity for Amino-terminal Acetylation: DETAILED STRUCTURAL AND BIOCHEMICAL ANALYSIS USING TETRAPEPTIDE LIBRARY.
The Journal of biological chemistry291(39)20530-8(2016 09)
Reddi R,Saddanapu V,Chinthapalli DK,Sankoju P,Sripadi P,Addlagatta A
Opposing Functions of the N-terminal Acetyltransferases Naa50 and NatA in Sister-chromatid Cohesion.
The Journal of biological chemistry291(36)19079-91(2016 09)
Rong Z,Ouyang Z,Magin RS,Marmorstein R,Yu H
N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
Proteomics15(14)2436-46(2015 Jul)
Van Damme P,Hole K,Gevaert K,Arnesen T
An organellar nα-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
Cell reports10(8)1362-74(2015 Mar)
Aksnes H,Van Damme P,Goris M,Starheim KK,Marie M,Støve SI,Hoel C,Kalvik TV,Hole K,Glomnes N,Furnes C,Ljostveit S,Ziegler M,Niere M,Gevaert K,Arnesen T
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