WEE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	WEE1
Entrez Gene ID	7465
Full Name	WEE1 G2 checkpoint kinase
Synonyms	WEE1A,WEE1hu
General protein information	Preferred Names WEE1 G2 checkpoint kinase Names wee1-like protein kinase WEE1 homolog WEE1+ homolog wee1A kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.4
Summary	This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008].
Disorder MIM:	193525

mRNA and Protein(s)

mRNA	Protein	Name
NM_001143976.1	NP_001137448.1	wee1-like protein kinase isoform 2
NM_003390.3	NP_003381.1	wee1-like protein kinase isoform 1
NM_003390.4	NP_003381.1	wee1-like protein kinase isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wee1	NP_033542.2
Rattus norvegicus (Norway rat)	Wee1	NP_001012760.1
Danio rerio (zebrafish)	wee1	NP_001005770.1
Homo sapiens (human)	WEE1	NP_003381.1
Macaca mulatta (Rhesus monkey)	WEE1	XP_002808123.1
Drosophila melanogaster (fruit fly)	wee	NP_001260167.1
Pan troglodytes (chimpanzee)	WEE1	XP_521839.2
Canis lupus familiaris (dog)	WEE1	XP_534051.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007798	XP_317709.4
Gallus gallus (chicken)	WEE1	NP_001026352.2

Gene Ontology
Bibliography

Related articles in PubMed

Oncogenic splicing abnormalities induced by DEAD-Box Helicase 56 amplification in colorectal cancer.
Kouyama Y, Masuda T, Fujii A, Ogawa Y, Sato K, Tobo T, Wakiyama H, Yoshikawa Y, Noda M, Tsuruda Y, Kuroda Y, Eguchi H, Ishida F, Kudo SE, Mimori K
Cancer science110(10)3132-3144(2019 Oct)

LncRNA SNHG3 regulates laryngeal carcinoma proliferation and migration by modulating the miR-384/WEE1 axis.
Wang L, Su K, Wu H, Li J, Song D
Life sciences232116597(2019 Sep)

C/EBPβ Is a Transcriptional Regulator of Wee1 at the G₂/M Phase of the Cell Cycle.
Lee JH, Sung JY, Choi EK, Yoon HK, Kang BR, Hong EK, Park BK, Kim YN, Rho SB, Yoon K
Cells8(2)(2019 02)

Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR-101-3p/WEE1 axis.
Chen X, Zhang N
Cell biology international43(1)44-55(2019 Jan)

Association between MicroRNA-373 and Long Noncoding RNA NORAD in Hepatitis C Virus-Infected Hepatocytes Impairs Wee1 Expression for Growth Promotion.
Sur S, Sasaki R, Devhare P, Steele R, Ray R, Ray RB
Journal of virology92(20)(2018 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WEE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WEE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17659	NM_001143976.1 Latest version!	Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu17505	NM_003390.4 Latest version!	Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu17505	NM_003390.3	Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WEE1 ( NM_001143976.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137448.1 Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17659
Clone ID Related Accession (Same CDS sequence)	NM_001143976.1
Accession Version	NM_001143976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	wee1-like protein kinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122837.1 and BQ024511.1. Transcript Variant: This variant (2) has a different first exon and 5' UTR, compared to variant 1. This difference causes translation initiation from an in-frame downstream AUG and an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATACAG AAAAATCAGG AAAAAGGGAA TTTGATGTGC GACAGACTCC TCAAGTGAAT 
ATTAATCCTT TTACTCCGGA TTCTTTGTTG CTTCATTCCT CAGGACAGTG TCGTCGTAGA 
AAGAGAACGT ATTGGAATGA TTCCTGTGGT GAAGACATGG AAGCCAGTGA TTATGAGCTT 
GAAGATGAAA CAAGACCTGC TAAGAGAATT ACAATTACTG AAAGCAATAT GAAGTCCCGG 
TATACAACAG AATTTCATGA GCTAGAGAAA ATCGGCTCTG GAGAATTTGG TTCTGTATTT 
AAGTGTGTGA AGAGGCTGGA TGGATGCATT TATGCCATTA AGCGATCAAA AAAGCCATTG 
GCGGGCTCTG TTGATGAGCA GAACGCTTTG AGAGAAGTAT ATGCTCATGC AGTGCTTGGA 
CAGCATTCTC ATGTAGTTCG ATATTTCTCT GCGTGGGCAG AAGATGATCA TATGCTTATA 
CAGAATGAAT ATTGTAATGG TGGAAGTTTA GCTGATGCTA TAAGTGAAAA CTACAGAATC 
ATGAGTTACT TTAAAGAAGC AGAGTTGAAG GATCTCCTTT TGCAAGTTGG CCGAGGCTTG 
AGGTATATTC ATTCAATGTC TTTGGTTCAC ATGGATATAA AACCTAGTAA TATTTTCATA 
TCTCGAACCT CAATCCCAAA TGCTGCCTCT GAAGAAGGAG ACGAAGATGA TTGGGCATCC 
AACAAAGTTA TGTTTAAAAT AGGTGATCTT GGGCATGTAA CAAGGATCTC CAGTCCACAA 
GTTGAAGAGG GCGATAGTCG TTTTCTTGCA AATGAAGTTT TACAGGAGAA TTATACCCAT 
CTACCAAAAG CAGATATTTT TGCGCTTGCC CTCACAGTGG TATGTGCTGC TGGTGCTGAA 
CCTCTTCCGA GAAATGGAGA TCAATGGCAT GAAATCAGAC AGGGTAGATT ACCTCGGATA 
CCACAAGTGC TTTCCCAAGA ATTTACAGAG TTGCTAAAAG TTATGATTCA TCCAGATCCA 
GAGAGAAGAC CTTCAGCAAT GGCACTGGTA AAGCATTCAG TATTGCTGTC CGCTTCTAGA 
AAGAGTGCAG AACAATTACG AATAGAATTG AATGCCGAAA AGTTCAAAAA TTCACTTTTA 
CAAAAAGAAC TCAAGAAAGC ACAGATGGCA AAAGCTGCAG CTGAGGAAAG AGCACTCTTC 
ACTGACCGGA TGGCCACTAG GTCCACCACC CAGAGTAATA GAACATCTCG ACTTATTGGA 
AAGAAAATGA ACCGCTCTGT CAGCCTTACT ATATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137448.1
CDS	756..2054
Translation	MDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Target ORF information:

RefSeq Version	NM_001143976.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143976.1

ORF Insert Sequence:

ATGGATACAG AAAAATCAGG AAAAAGGGAA TTTGATGTGC GACAGACTCC TCAAGTGAAT 
ATTAATCCTT TTACTCCGGA TTCTTTGTTG CTTCATTCCT CAGGACAGTG TCGTCGTAGA 
AAGAGAACGT ATTGGAATGA TTCCTGTGGT GAAGACATGG AAGCCAGTGA TTATGAGCTT 
GAAGATGAAA CAAGACCTGC TAAGAGAATT ACAATTACTG AAAGCAATAT GAAGTCCCGG 
TATACAACAG AATTTCATGA GCTAGAGAAA ATCGGCTCTG GAGAATTTGG TTCTGTATTT 
AAGTGTGTGA AGAGGCTGGA TGGATGCATT TATGCCATTA AGCGATCAAA AAAGCCATTG 
GCGGGCTCTG TTGATGAGCA GAACGCTTTG AGAGAAGTAT ATGCTCATGC AGTGCTTGGA 
CAGCATTCTC ATGTAGTTCG ATATTTCTCT GCGTGGGCAG AAGATGATCA TATGCTTATA 
CAGAATGAAT ATTGTAATGG TGGAAGTTTA GCTGATGCTA TAAGTGAAAA CTACAGAATC 
ATGAGTTACT TTAAAGAAGC AGAGTTGAAG GATCTCCTTT TGCAAGTTGG CCGAGGCTTG 
AGGTATATTC ATTCAATGTC TTTGGTTCAC ATGGATATAA AACCTAGTAA TATTTTCATA 
TCTCGAACCT CAATCCCAAA TGCTGCCTCT GAAGAAGGAG ACGAAGATGA TTGGGCATCC 
AACAAAGTTA TGTTTAAAAT AGGTGATCTT GGGCATGTAA CAAGGATCTC CAGTCCACAA 
GTTGAAGAGG GCGATAGTCG TTTTCTTGCA AATGAAGTTT TACAGGAGAA TTATACCCAT 
CTACCAAAAG CAGATATTTT TGCGCTTGCC CTCACAGTGG TATGTGCTGC TGGTGCTGAA 
CCTCTTCCGA GAAATGGAGA TCAATGGCAT GAAATCAGAC AGGGTAGATT ACCTCGGATA 
CCACAAGTGC TTTCCCAAGA ATTTACAGAG TTGCTAAAAG TTATGATTCA TCCAGATCCA 
GAGAGAAGAC CTTCAGCAAT GGCACTGGTA AAGCATTCAG TATTGCTGTC CGCTTCTAGA 
AAGAGTGCAG AACAATTACG AATAGAATTG AATGCCGAAA AGTTCAAAAA TTCACTTTTA 
CAAAAAGAAC TCAAGAAAGC ACAGATGGCA AAAGCTGCAG CTGAGGAAAG AGCACTCTTC 
ACTGACCGGA TGGCCACTAG GTCCACCACC CAGAGTAATA GAACATCTCG ACTTATTGGA 
AAGAAAATGA ACCGCTCTGT CAGCCTTACT ATATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WEE1 ( NM_003390.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003381.1 Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17505
Clone ID Related Accession (Same CDS sequence)	NM_003390.3 , NM_003390.4
Accession Version	NM_003390.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Homo sapiens(human)
Product	wee1-like protein kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U10564.1, BC051831.1 and BQ024511.1. On Jan 24, 2009 this sequence version replaced NM_003390.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10564.1, BC070052.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCACCCCGCC GCGCCGGGGC GGCCTGCACC 
TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGACTGTG AGGAGGAGGA AGAAGAGGAG 
GAGGAGGAGG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAAGA GCCCGACTCG 
CCGCTGCCGC CCGCGCGGAG CCCCACGGAG CCCGGGCCCG AGCGCCGCCG CTCGCCCGGG 
CCGGCCCCCG GCAGCCCCGG CGAGCTGGAG GAGGACCTGT TGCTGCCCGG CGCCTGCCCG 
GGCGCGGACG AGGCGGGCGG TGGGGCGGAG GGCGACTCGT GGGAGGAGGA GGGCTTCGGC 
TCCTCGTCGC CGGTCAAGTC GCCGGCGGCC CCCTACTTCC TGGGTAGCTC TTTCTCGCCG 
GTGCGCTGCG GCGGCCCAGG AGATGCGTCG CCGCGGGGTT GCGGGGCGCG CCGGGCGGGC 
GAAGGCCGCC GCTCGCCGCG GCCGGACCAC CCGGGCACCC CGCCACACAA GACCTTCCGC 
AAGCTGCGAC TCTTCGACAC CCCGCACACG CCCAAGAGTT TGCTCTCCAA AGCTCGGGGA 
ATTGATTCCA GCTCTGTTAA ACTCCGGGGT AGTTCTCTCT TCATGGATAC AGAAAAATCA 
GGAAAAAGGG AATTTGATGT GCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCG 
GATTCTTTGT TGCTTCATTC CTCAGGACAG TGTCGTCGTA GAAAGAGAAC GTATTGGAAT 
GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGC TTGAAGATGA AACAAGACCT 
GCTAAGAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCCC GGTATACAAC AGAATTTCAT 
GAGCTAGAGA AAATCGGCTC TGGAGAATTT GGTTCTGTAT TTAAGTGTGT GAAGAGGCTG 
GATGGATGCA TTTATGCCAT TAAGCGATCA AAAAAGCCAT TGGCGGGCTC TGTTGATGAG 
CAGAACGCTT TGAGAGAAGT ATATGCTCAT GCAGTGCTTG GACAGCATTC TCATGTAGTT 
CGATATTTCT CTGCGTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAT 
GGTGGAAGTT TAGCTGATGC TATAAGTGAA AACTACAGAA TCATGAGTTA CTTTAAAGAA 
GCAGAGTTGA AGGATCTCCT TTTGCAAGTT GGCCGAGGCT TGAGGTATAT TCATTCAATG 
TCTTTGGTTC ACATGGATAT AAAACCTAGT AATATTTTCA TATCTCGAAC CTCAATCCCA 
AATGCTGCCT CTGAAGAAGG AGACGAAGAT GATTGGGCAT CCAACAAAGT TATGTTTAAA 
ATAGGTGATC TTGGGCATGT AACAAGGATC TCCAGTCCAC AAGTTGAAGA GGGCGATAGT 
CGTTTTCTTG CAAATGAAGT TTTACAGGAG AATTATACCC ATCTACCAAA AGCAGATATT 
TTTGCGCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AACCTCTTCC GAGAAATGGA 
GATCAATGGC ATGAAATCAG ACAGGGTAGA TTACCTCGGA TACCACAAGT GCTTTCCCAA 
GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
ATGGCACTGG TAAAGCATTC AGTATTGCTG TCCGCTTCTA GAAAGAGTGC AGAACAATTA 
CGAATAGAAT TGAATGCCGA AAAGTTCAAA AATTCACTTT TACAAAAAGA ACTCAAGAAA 
GCACAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGACCG GATGGCCACT 
AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
GTCAGCCTTA CTATATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003381.1
CDS	254..2194
Translation	MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Target ORF information:

RefSeq Version	NM_003390.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003390.3

ORF Insert Sequence:

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCACCCCGCC GCGCCGGGGC GGCCTGCACC 
TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGACTGTG AGGAGGAGGA AGAAGAGGAG 
GAGGAGGAGG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAAGA GCCCGACTCG 
CCGCTGCCGC CCGCGCGGAG CCCCACGGAG CCCGGGCCCG AGCGCCGCCG CTCGCCCGGG 
CCGGCCCCCG GCAGCCCCGG CGAGCTGGAG GAGGACCTGT TGCTGCCCGG CGCCTGCCCG 
GGCGCGGACG AGGCGGGCGG TGGGGCGGAG GGCGACTCGT GGGAGGAGGA GGGCTTCGGC 
TCCTCGTCGC CGGTCAAGTC GCCGGCGGCC CCCTACTTCC TGGGTAGCTC TTTCTCGCCG 
GTGCGCTGCG GCGGCCCAGG AGATGCGTCG CCGCGGGGTT GCGGGGCGCG CCGGGCGGGC 
GAAGGCCGCC GCTCGCCGCG GCCGGACCAC CCGGGCACCC CGCCACACAA GACCTTCCGC 
AAGCTGCGAC TCTTCGACAC CCCGCACACG CCCAAGAGTT TGCTCTCCAA AGCTCGGGGA 
ATTGATTCCA GCTCTGTTAA ACTCCGGGGT AGTTCTCTCT TCATGGATAC AGAAAAATCA 
GGAAAAAGGG AATTTGATGT GCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCG 
GATTCTTTGT TGCTTCATTC CTCAGGACAG TGTCGTCGTA GAAAGAGAAC GTATTGGAAT 
GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGC TTGAAGATGA AACAAGACCT 
GCTAAGAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCCC GGTATACAAC AGAATTTCAT 
GAGCTAGAGA AAATCGGCTC TGGAGAATTT GGTTCTGTAT TTAAGTGTGT GAAGAGGCTG 
GATGGATGCA TTTATGCCAT TAAGCGATCA AAAAAGCCAT TGGCGGGCTC TGTTGATGAG 
CAGAACGCTT TGAGAGAAGT ATATGCTCAT GCAGTGCTTG GACAGCATTC TCATGTAGTT 
CGATATTTCT CTGCGTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAT 
GGTGGAAGTT TAGCTGATGC TATAAGTGAA AACTACAGAA TCATGAGTTA CTTTAAAGAA 
GCAGAGTTGA AGGATCTCCT TTTGCAAGTT GGCCGAGGCT TGAGGTATAT TCATTCAATG 
TCTTTGGTTC ACATGGATAT AAAACCTAGT AATATTTTCA TATCTCGAAC CTCAATCCCA 
AATGCTGCCT CTGAAGAAGG AGACGAAGAT GATTGGGCAT CCAACAAAGT TATGTTTAAA 
ATAGGTGATC TTGGGCATGT AACAAGGATC TCCAGTCCAC AAGTTGAAGA GGGCGATAGT 
CGTTTTCTTG CAAATGAAGT TTTACAGGAG AATTATACCC ATCTACCAAA AGCAGATATT 
TTTGCGCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AACCTCTTCC GAGAAATGGA 
GATCAATGGC ATGAAATCAG ACAGGGTAGA TTACCTCGGA TACCACAAGT GCTTTCCCAA 
GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
ATGGCACTGG TAAAGCATTC AGTATTGCTG TCCGCTTCTA GAAAGAGTGC AGAACAATTA 
CGAATAGAAT TGAATGCCGA AAAGTTCAAA AATTCACTTT TACAAAAAGA ACTCAAGAAA 
GCACAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGACCG GATGGCCACT 
AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
GTCAGCCTTA CTATATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WEE1 ( NM_003390.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003381.1 Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17505
Clone ID Related Accession (Same CDS sequence)	NM_003390.3 , NM_003390.4
Accession Version	NM_003390.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	wee1-like protein kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122179.6, U10564.1, BC051831.1, BQ024511.1 and AC011979.7. On Nov 22, 2018 this sequence version replaced NM_003390.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051831.1, X62048.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000450114.7/ ENSP00000402084.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCACCCCGCC GCGCCGGGGC GGCCTGCACC 
TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGACTGTG AGGAGGAGGA AGAAGAGGAG 
GAGGAGGAGG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAAGA GCCCGACTCG 
CCGCTGCCGC CCGCGCGGAG CCCCACGGAG CCCGGGCCCG AGCGCCGCCG CTCGCCCGGG 
CCGGCCCCCG GCAGCCCCGG CGAGCTGGAG GAGGACCTGT TGCTGCCCGG CGCCTGCCCG 
GGCGCGGACG AGGCGGGCGG TGGGGCGGAG GGCGACTCGT GGGAGGAGGA GGGCTTCGGC 
TCCTCGTCGC CGGTCAAGTC GCCGGCGGCC CCCTACTTCC TGGGTAGCTC TTTCTCGCCG 
GTGCGCTGCG GCGGCCCAGG AGATGCGTCG CCGCGGGGTT GCGGGGCGCG CCGGGCGGGC 
GAAGGCCGCC GCTCGCCGCG GCCGGACCAC CCGGGCACCC CGCCACACAA GACCTTCCGC 
AAGCTGCGAC TCTTCGACAC CCCGCACACG CCCAAGAGTT TGCTCTCCAA AGCTCGGGGA 
ATTGATTCCA GCTCTGTTAA ACTCCGGGGT AGTTCTCTCT TCATGGATAC AGAAAAATCA 
GGAAAAAGGG AATTTGATGT GCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCG 
GATTCTTTGT TGCTTCATTC CTCAGGACAG TGTCGTCGTA GAAAGAGAAC GTATTGGAAT 
GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGC TTGAAGATGA AACAAGACCT 
GCTAAGAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCCC GGTATACAAC AGAATTTCAT 
GAGCTAGAGA AAATCGGCTC TGGAGAATTT GGTTCTGTAT TTAAGTGTGT GAAGAGGCTG 
GATGGATGCA TTTATGCCAT TAAGCGATCA AAAAAGCCAT TGGCGGGCTC TGTTGATGAG 
CAGAACGCTT TGAGAGAAGT ATATGCTCAT GCAGTGCTTG GACAGCATTC TCATGTAGTT 
CGATATTTCT CTGCGTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAT 
GGTGGAAGTT TAGCTGATGC TATAAGTGAA AACTACAGAA TCATGAGTTA CTTTAAAGAA 
GCAGAGTTGA AGGATCTCCT TTTGCAAGTT GGCCGAGGCT TGAGGTATAT TCATTCAATG 
TCTTTGGTTC ACATGGATAT AAAACCTAGT AATATTTTCA TATCTCGAAC CTCAATCCCA 
AATGCTGCCT CTGAAGAAGG AGACGAAGAT GATTGGGCAT CCAACAAAGT TATGTTTAAA 
ATAGGTGATC TTGGGCATGT AACAAGGATC TCCAGTCCAC AAGTTGAAGA GGGCGATAGT 
CGTTTTCTTG CAAATGAAGT TTTACAGGAG AATTATACCC ATCTACCAAA AGCAGATATT 
TTTGCGCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AACCTCTTCC GAGAAATGGA 
GATCAATGGC ATGAAATCAG ACAGGGTAGA TTACCTCGGA TACCACAAGT GCTTTCCCAA 
GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
ATGGCACTGG TAAAGCATTC AGTATTGCTG TCCGCTTCTA GAAAGAGTGC AGAACAATTA 
CGAATAGAAT TGAATGCCGA AAAGTTCAAA AATTCACTTT TACAAAAAGA ACTCAAGAAA 
GCACAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGACCG GATGGCCACT 
AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
GTCAGCCTTA CTATATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003381.1
CDS	265..2205
Translation	MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Target ORF information:

RefSeq Version	NM_003390.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003390.4

ORF Insert Sequence:

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCACCCCGCC GCGCCGGGGC GGCCTGCACC 
TTGCGGCAGA AGCTGATCTT CTCGCCCTGC AGCGACTGTG AGGAGGAGGA AGAAGAGGAG 
GAGGAGGAGG GCAGCGGCCA CAGCACCGGG GAGGACTCGG CCTTTCAAGA GCCCGACTCG 
CCGCTGCCGC CCGCGCGGAG CCCCACGGAG CCCGGGCCCG AGCGCCGCCG CTCGCCCGGG 
CCGGCCCCCG GCAGCCCCGG CGAGCTGGAG GAGGACCTGT TGCTGCCCGG CGCCTGCCCG 
GGCGCGGACG AGGCGGGCGG TGGGGCGGAG GGCGACTCGT GGGAGGAGGA GGGCTTCGGC 
TCCTCGTCGC CGGTCAAGTC GCCGGCGGCC CCCTACTTCC TGGGTAGCTC TTTCTCGCCG 
GTGCGCTGCG GCGGCCCAGG AGATGCGTCG CCGCGGGGTT GCGGGGCGCG CCGGGCGGGC 
GAAGGCCGCC GCTCGCCGCG GCCGGACCAC CCGGGCACCC CGCCACACAA GACCTTCCGC 
AAGCTGCGAC TCTTCGACAC CCCGCACACG CCCAAGAGTT TGCTCTCCAA AGCTCGGGGA 
ATTGATTCCA GCTCTGTTAA ACTCCGGGGT AGTTCTCTCT TCATGGATAC AGAAAAATCA 
GGAAAAAGGG AATTTGATGT GCGACAGACT CCTCAAGTGA ATATTAATCC TTTTACTCCG 
GATTCTTTGT TGCTTCATTC CTCAGGACAG TGTCGTCGTA GAAAGAGAAC GTATTGGAAT 
GATTCCTGTG GTGAAGACAT GGAAGCCAGT GATTATGAGC TTGAAGATGA AACAAGACCT 
GCTAAGAGAA TTACAATTAC TGAAAGCAAT ATGAAGTCCC GGTATACAAC AGAATTTCAT 
GAGCTAGAGA AAATCGGCTC TGGAGAATTT GGTTCTGTAT TTAAGTGTGT GAAGAGGCTG 
GATGGATGCA TTTATGCCAT TAAGCGATCA AAAAAGCCAT TGGCGGGCTC TGTTGATGAG 
CAGAACGCTT TGAGAGAAGT ATATGCTCAT GCAGTGCTTG GACAGCATTC TCATGTAGTT 
CGATATTTCT CTGCGTGGGC AGAAGATGAT CATATGCTTA TACAGAATGA ATATTGTAAT 
GGTGGAAGTT TAGCTGATGC TATAAGTGAA AACTACAGAA TCATGAGTTA CTTTAAAGAA 
GCAGAGTTGA AGGATCTCCT TTTGCAAGTT GGCCGAGGCT TGAGGTATAT TCATTCAATG 
TCTTTGGTTC ACATGGATAT AAAACCTAGT AATATTTTCA TATCTCGAAC CTCAATCCCA 
AATGCTGCCT CTGAAGAAGG AGACGAAGAT GATTGGGCAT CCAACAAAGT TATGTTTAAA 
ATAGGTGATC TTGGGCATGT AACAAGGATC TCCAGTCCAC AAGTTGAAGA GGGCGATAGT 
CGTTTTCTTG CAAATGAAGT TTTACAGGAG AATTATACCC ATCTACCAAA AGCAGATATT 
TTTGCGCTTG CCCTCACAGT GGTATGTGCT GCTGGTGCTG AACCTCTTCC GAGAAATGGA 
GATCAATGGC ATGAAATCAG ACAGGGTAGA TTACCTCGGA TACCACAAGT GCTTTCCCAA 
GAATTTACAG AGTTGCTAAA AGTTATGATT CATCCAGATC CAGAGAGAAG ACCTTCAGCA 
ATGGCACTGG TAAAGCATTC AGTATTGCTG TCCGCTTCTA GAAAGAGTGC AGAACAATTA 
CGAATAGAAT TGAATGCCGA AAAGTTCAAA AATTCACTTT TACAAAAAGA ACTCAAGAAA 
GCACAGATGG CAAAAGCTGC AGCTGAGGAA AGAGCACTCT TCACTGACCG GATGGCCACT 
AGGTCCACCA CCCAGAGTAA TAGAACATCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
GTCAGCCTTA CTATATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oncogenic splicing abnormalities induced by DEAD-Box Helicase 56 amplification in colorectal cancer.
Cancer science110(10)3132-3144(2019 Oct)
Kouyama Y,Masuda T,Fujii A,Ogawa Y,Sato K,Tobo T,Wakiyama H,Yoshikawa Y,Noda M,Tsuruda Y,Kuroda Y,Eguchi H,Ishida F,Kudo SE,Mimori K

LncRNA SNHG3 regulates laryngeal carcinoma proliferation and migration by modulating the miR-384/WEE1 axis.
Life sciences232116597(2019 Sep)
Wang L,Su K,Wu H,Li J,Song D

C/EBPβ Is a Transcriptional Regulator of Wee1 at the G₂/M Phase of the Cell Cycle.
Cells8(2)(2019 02)
Lee JH,Sung JY,Choi EK,Yoon HK,Kang BR,Hong EK,Park BK,Kim YN,Rho SB,Yoon K

Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR-101-3p/WEE1 axis.
Cell biology international43(1)44-55(2019 Jan)
Chen X,Zhang N

Association between MicroRNA-373 and Long Noncoding RNA NORAD in Hepatitis C Virus-Infected Hepatocytes Impairs Wee1 Expression for Growth Promotion.
Journal of virology92(20)(2018 10)
Sur S,Sasaki R,Devhare P,Steele R,Ray R,Ray RB

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WEE1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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