| Gene Symbol | UBA1 |
| Entrez Gene ID | 7317 |
| Full Name | ubiquitin like modifier activating enzyme 1 |
| Synonyms | A1S9,A1S9T,A1ST,AMCX1,CFAP124,GXP1,POC20,SMAX2,UBA1A,UBE1,UBE1X |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: | |
| Disorder Html: | Spinal muscular atrophy, X-linked 2, infantile, 301830 (3) |
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UBA1 cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following UBA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu54021 Clone ID Related Accession (Same CDS sequence) XM_017029779.2 , XM_005272649.1 , XM_017029779.1 Accession Version XM_017029779.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X4 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGAGATCTC TACCCCAGAT GTCCAGCTCG CCGCTGTCCA AGAAACGTCG CGTGTCCGGG
CCTGATCCAA AGCCGGGTTC TAACTGCTCC CCTGCCCAGT CCGTGTTGTC CGAAGTGCCC
TCGGTGCCAA CCAACGGAAT GGCCAAGAAC GGCAGTGAAG CAGACATAGA CGAGGGCCTT
TACTCCCGGC AGCTGTATGT GTTGGGCCAT GAGGCAATGA AGCGGCTCCA GACATCCAGT
GTCCTGGTAT CAGGCCTGCG GGGCCTGGGC GTGGAGATCG CTAAGAACAT CATCCTTGGT
GGGGTCAAGG CTGTTACCCT ACATGACCAG GGCACTGCCC AGTGGGCTGA TCTTTCCTCC
CAGTTCTACC TGCGGGAGGA GGACATCGGT AAAAACCGGG CCGAGGTATC ACAGCCCCGC
CTCGCTGAGC TCAACAGCTA TGTGCCTGTC ACTGCCTACA CTGGACCCCT CGTTGAGGAC
TTCCTTAGTG GTTTCCAGGT GGTGGTGCTC ACCAACACCC CCCTGGAGGA CCAGCTGCGA
GTGGGTGAGT TCTGTCACAA CCGTGGCATC AAGCTGGTGG TGGCAGACAC GCGGGGCCTG
TTTGGGCAGC TCTTCTGTGA CTTTGGAGAG GAAATGATCC TCACAGATTC CAATGGGGAG
CAGCCACTCA GTGCTATGGT TTCTATGGTT ACCAAGGACA ACCCCGGTGT GGTTACCTGC
CTGGATGAGG CCCGACACGG GTTTGAGAGC GGGGACTTTG TCTCCTTTTC AGAAGTACAG
GGCATGGTTG AACTCAACGG AAATCAGCCC ATGGAGATCA AAGTCCTGGG TCCTTATACC
TTTAGCATCT GTGACACCTC CAACTTCTCC GACTACATCC GTGGAGGCAT CGTCAGTCAG
GTCAAAGTAC CTAAGAAGAT TAGCTTTAAA TCCTTGGTGG CCTCACTGGC AGAACCTGAC
TTTGTGGTGA CGGACTTCGC CAAGTTTTCT CGCCCTGCCC AGCTGCACAT TGGCTTCCAG
GCCCTGCACC AGTTCTGTGC TCAGCATGGC CGGCCACCTC GGCCCCGCAA TGAGGAGGAT
GCAGCAGAAC TGGTAGCCTT AGCACAGGCT GTGAATGCTC GAGCCCTGCC AGCAGTGCAG
CAAAATAACC TGGACGAGGA CCTCATCCGG AAGCTGGCAT ATGTGGCTGC TGGGGATCTG
GCACCCATAA ACGCCTTCAT TGGGGGCCTG GCTGCCCAGG AAGTCATGAA GGCCTGCTCC
GGGAAGTTCA TGCCCATCAT GCAGTGGCTA TACTTTGATG CCCTTGAGTG TCTCCCTGAG
GACAAAGAGG TCCTCACAGA GGACAAGTGC CTCCAGCGCC AGAACCGTTA TGACGGGCAA
GTGGCTGTGT TTGGCTCAGA CCTGCAAGAG AAGCTGGGCA AGCAGAAGTA TTTCCTGGTG
GGTGCGGGGG CCATTGGCTG TGAGCTGCTC AAGAACTTTG CCATGATTGG GCTGGGCTGC
GGGGAGGGTG GAGAAATCAT CGTTACAGAC ATGGACACCA TTGAGAAGTC AAATCTGAAT
CGACAGTTTC TTTTCCGGCC CTGGGATGTC ACGAAGTTAA AGTCTGACAC GGCTGCTGCA
GCTGTGCGCC AAATGAATCC ACATATCCGG GTGACAAGCC ACCAGAACCG TGTGGGTCCT
GACACGGAGC GCATCTATGA TGACGATTTT TTCCAAAACC TAGATGGCGT GGCCAATGCC
CTGGACAACG TGGATGCCCG CATGTACATG GACCGCCGCT GTGTCTACTA CCGGAAGCCA
CTGCTGGAGT CAGGCACACT GGGCACCAAA GGCAATGTGC AGGTGGTGAT CCCCTTCCTG
ACAGAGTCGT ACAGTTCCAG CCAGGACCCA CCTGAGAAGT CCATCCCCAT CTGTACCCTG
AAGAACTTCC CTAATGCCAT CGAGCACACC CTGCAGTGGG CTCGGGATGA GTTTGAAGGC
CTCTTCAAGC AGCCAGCAGA AAATGTCAAC CAGTACCTCA CAGACCCCAA GTTTGTGGAG
CGAACACTGC GGCTGGCAGG CACTCAGCCC TTGGAGGTGC TGGAGGCTGT GCAGCGCAGC
CTGGTGCTGC AGCGACCACA GACCTGGGCT GACTGCGTGA CCTGGGCCTG CCACCACTGG
CACACCCAGT ACTCGAACAA CATCCGGCAG CTGCTGCACA ACTTCCCTCC TGACCAGCTC
ACAAGCTCAG GAGCGCCGTT CTGGTCTGGG CCCAAACGCT GTCCACACCC GCTCACCTTT
GATGTCAACA ATCCCCTGCA TCTGGACTAT GTGATGGCTG CTGCCAACCT GTTTGCCCAG
ACCTACGGGC TGACAGGCTC TCAGGACCGA GCTGCTGTGG CCACATTCCT GCAGTCTGTG
CAGGTCCCCG AATTCACCCC CAAGTCTGGC GTCAAGATCC ATGTTTCTGA CCAGGAGCTG
CAGAGCGCCA ATGCCTCTGT TGATGACAGT CGTCTAGAGG AGCTCAAAGC CACTCTGCCC
AGCCCAGACA AGCTCCCTGG ATTCAAGATG TACCCCATTG ACTTTGAGAA GGATGATGAC
AGCAACTTTC ATATGGATTT CATCGTGGCT GCATCCAACC TCCGGGCAGA AAACTATGAC
ATTCCTTCTG CAGACCGGCA CAAGAGCAAG CTGATTGCAG GGAAGATCAT CCCAGCCATT
GCCACGACCA CAGCAGCCGT GGTTGGCCTT GTGTGTCTGG AGCTGTACAA GGTTGTGCAG
GGGCACCGAC AGCTTGACTC CTACAAGAAT GGTTTCCTCA ACTTGGCCCT GCCTTTCTTT
GGTTTCTCTG AACCCCTTGC CGCACCACGT CACCAGTACT ATAACCAAGA GTGGACATTG
TGGGATCGCT TTGAGGTACA AGGGCTGCAG CCTAATGGTG AGGAGATGAC CCTCAAACAG
TTCCTCGACT ATTTTAAGAC AGAGCACAAA TTAGAGATCA CCATGCTGTC CCAGGGCGTG
TCCATGCTCT ATTCCTTCTT CATGCCAGCT GCCAAGCTCA AGGAACGGTT GGATCAGCCG
ATGACAGAGA TTGTGAGCCG TGTGTCGAAG CGAAAGCTGG GCCGCCACGT GCGGGCGCTG
GTGCTTGAGC TGTGCTGTAA CGACGAGAGC GGCGAGGATG TCGAGGTTCC CTATGTCCGA
TACACCATCC GCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016885268.1 CDS 2914..6108 Translation 
Target ORF information:
RefSeq Version XM_017029779.2 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
XM_017029779.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGAGATCTC TACCCCAGAT GTCCAGCTCG CCGCTGTCCA AGAAACGTCG CGTGTCCGGG
CCTGATCCAA AGCCGGGTTC TAACTGCTCC CCTGCCCAGT CCGTGTTGTC CGAAGTGCCC
TCGGTGCCAA CCAACGGAAT GGCCAAGAAC GGCAGTGAAG CAGACATAGA CGAGGGCCTT
TACTCCCGGC AGCTGTATGT GTTGGGCCAT GAGGCAATGA AGCGGCTCCA GACATCCAGT
GTCCTGGTAT CAGGCCTGCG GGGCCTGGGC GTGGAGATCG CTAAGAACAT CATCCTTGGT
GGGGTCAAGG CTGTTACCCT ACATGACCAG GGCACTGCCC AGTGGGCTGA TCTTTCCTCC
CAGTTCTACC TGCGGGAGGA GGACATCGGT AAAAACCGGG CCGAGGTATC ACAGCCCCGC
CTCGCTGAGC TCAACAGCTA TGTGCCTGTC ACTGCCTACA CTGGACCCCT CGTTGAGGAC
TTCCTTAGTG GTTTCCAGGT GGTGGTGCTC ACCAACACCC CCCTGGAGGA CCAGCTGCGA
GTGGGTGAGT TCTGTCACAA CCGTGGCATC AAGCTGGTGG TGGCAGACAC GCGGGGCCTG
TTTGGGCAGC TCTTCTGTGA CTTTGGAGAG GAAATGATCC TCACAGATTC CAATGGGGAG
CAGCCACTCA GTGCTATGGT TTCTATGGTT ACCAAGGACA ACCCCGGTGT GGTTACCTGC
CTGGATGAGG CCCGACACGG GTTTGAGAGC GGGGACTTTG TCTCCTTTTC AGAAGTACAG
GGCATGGTTG AACTCAACGG AAATCAGCCC ATGGAGATCA AAGTCCTGGG TCCTTATACC
TTTAGCATCT GTGACACCTC CAACTTCTCC GACTACATCC GTGGAGGCAT CGTCAGTCAG
GTCAAAGTAC CTAAGAAGAT TAGCTTTAAA TCCTTGGTGG CCTCACTGGC AGAACCTGAC
TTTGTGGTGA CGGACTTCGC CAAGTTTTCT CGCCCTGCCC AGCTGCACAT TGGCTTCCAG
GCCCTGCACC AGTTCTGTGC TCAGCATGGC CGGCCACCTC GGCCCCGCAA TGAGGAGGAT
GCAGCAGAAC TGGTAGCCTT AGCACAGGCT GTGAATGCTC GAGCCCTGCC AGCAGTGCAG
CAAAATAACC TGGACGAGGA CCTCATCCGG AAGCTGGCAT ATGTGGCTGC TGGGGATCTG
GCACCCATAA ACGCCTTCAT TGGGGGCCTG GCTGCCCAGG AAGTCATGAA GGCCTGCTCC
GGGAAGTTCA TGCCCATCAT GCAGTGGCTA TACTTTGATG CCCTTGAGTG TCTCCCTGAG
GACAAAGAGG TCCTCACAGA GGACAAGTGC CTCCAGCGCC AGAACCGTTA TGACGGGCAA
GTGGCTGTGT TTGGCTCAGA CCTGCAAGAG AAGCTGGGCA AGCAGAAGTA TTTCCTGGTG
GGTGCGGGGG CCATTGGCTG TGAGCTGCTC AAGAACTTTG CCATGATTGG GCTGGGCTGC
GGGGAGGGTG GAGAAATCAT CGTTACAGAC ATGGACACCA TTGAGAAGTC AAATCTGAAT
CGACAGTTTC TTTTCCGGCC CTGGGATGTC ACGAAGTTAA AGTCTGACAC GGCTGCTGCA
GCTGTGCGCC AAATGAATCC ACATATCCGG GTGACAAGCC ACCAGAACCG TGTGGGTCCT
GACACGGAGC GCATCTATGA TGACGATTTT TTCCAAAACC TAGATGGCGT GGCCAATGCC
CTGGACAACG TGGATGCCCG CATGTACATG GACCGCCGCT GTGTCTACTA CCGGAAGCCA
CTGCTGGAGT CAGGCACACT GGGCACCAAA GGCAATGTGC AGGTGGTGAT CCCCTTCCTG
ACAGAGTCGT ACAGTTCCAG CCAGGACCCA CCTGAGAAGT CCATCCCCAT CTGTACCCTG
AAGAACTTCC CTAATGCCAT CGAGCACACC CTGCAGTGGG CTCGGGATGA GTTTGAAGGC
CTCTTCAAGC AGCCAGCAGA AAATGTCAAC CAGTACCTCA CAGACCCCAA GTTTGTGGAG
CGAACACTGC GGCTGGCAGG CACTCAGCCC TTGGAGGTGC TGGAGGCTGT GCAGCGCAGC
CTGGTGCTGC AGCGACCACA GACCTGGGCT GACTGCGTGA CCTGGGCCTG CCACCACTGG
CACACCCAGT ACTCGAACAA CATCCGGCAG CTGCTGCACA ACTTCCCTCC TGACCAGCTC
ACAAGCTCAG GAGCGCCGTT CTGGTCTGGG CCCAAACGCT GTCCACACCC GCTCACCTTT
GATGTCAACA ATCCCCTGCA TCTGGACTAT GTGATGGCTG CTGCCAACCT GTTTGCCCAG
ACCTACGGGC TGACAGGCTC TCAGGACCGA GCTGCTGTGG CCACATTCCT GCAGTCTGTG
CAGGTCCCCG AATTCACCCC CAAGTCTGGC GTCAAGATCC ATGTTTCTGA CCAGGAGCTG
CAGAGCGCCA ATGCCTCTGT TGATGACAGT CGTCTAGAGG AGCTCAAAGC CACTCTGCCC
AGCCCAGACA AGCTCCCTGG ATTCAAGATG TACCCCATTG ACTTTGAGAA GGATGATGAC
AGCAACTTTC ATATGGATTT CATCGTGGCT GCATCCAACC TCCGGGCAGA AAACTATGAC
ATTCCTTCTG CAGACCGGCA CAAGAGCAAG CTGATTGCAG GGAAGATCAT CCCAGCCATT
GCCACGACCA CAGCAGCCGT GGTTGGCCTT GTGTGTCTGG AGCTGTACAA GGTTGTGCAG
GGGCACCGAC AGCTTGACTC CTACAAGAAT GGTTTCCTCA ACTTGGCCCT GCCTTTCTTT
GGTTTCTCTG AACCCCTTGC CGCACCACGT CACCAGTACT ATAACCAAGA GTGGACATTG
TGGGATCGCT TTGAGGTACA AGGGCTGCAG CCTAATGGTG AGGAGATGAC CCTCAAACAG
TTCCTCGACT ATTTTAAGAC AGAGCACAAA TTAGAGATCA CCATGCTGTC CCAGGGCGTG
TCCATGCTCT ATTCCTTCTT CATGCCAGCT GCCAAGCTCA AGGAACGGTT GGATCAGCCG
ATGACAGAGA TTGTGAGCCG TGTGTCGAAG CGAAAGCTGG GCCGCCACGT GCGGGCGCTG
GTGCTTGAGC TGTGCTGTAA CGACGAGAGC GGCGAGGATG TCGAGGTTCC CTATGTCCGA
TACACCATCC GCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu54021 Clone ID Related Accession (Same CDS sequence) XM_017029779.2 , XM_005272649.1 , XM_017029779.1 Accession Version XM_005272649.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X4 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGAGATCTC TACCCCAGAT GTCCAGCTCG CCGCTGTCCA AGAAACGTCG CGTGTCCGGG
CCTGATCCAA AGCCGGGTTC TAACTGCTCC CCTGCCCAGT CCGTGTTGTC CGAAGTGCCC
TCGGTGCCAA CCAACGGAAT GGCCAAGAAC GGCAGTGAAG CAGACATAGA CGAGGGCCTT
TACTCCCGGC AGCTGTATGT GTTGGGCCAT GAGGCAATGA AGCGGCTCCA GACATCCAGT
GTCCTGGTAT CAGGCCTGCG GGGCCTGGGC GTGGAGATCG CTAAGAACAT CATCCTTGGT
GGGGTCAAGG CTGTTACCCT ACATGACCAG GGCACTGCCC AGTGGGCTGA TCTTTCCTCC
CAGTTCTACC TGCGGGAGGA GGACATCGGT AAAAACCGGG CCGAGGTATC ACAGCCCCGC
CTCGCTGAGC TCAACAGCTA TGTGCCTGTC ACTGCCTACA CTGGACCCCT CGTTGAGGAC
TTCCTTAGTG GTTTCCAGGT GGTGGTGCTC ACCAACACCC CCCTGGAGGA CCAGCTGCGA
GTGGGTGAGT TCTGTCACAA CCGTGGCATC AAGCTGGTGG TGGCAGACAC GCGGGGCCTG
TTTGGGCAGC TCTTCTGTGA CTTTGGAGAG GAAATGATCC TCACAGATTC CAATGGGGAG
CAGCCACTCA GTGCTATGGT TTCTATGGTT ACCAAGGACA ACCCCGGTGT GGTTACCTGC
CTGGATGAGG CCCGACACGG GTTTGAGAGC GGGGACTTTG TCTCCTTTTC AGAAGTACAG
GGCATGGTTG AACTCAACGG AAATCAGCCC ATGGAGATCA AAGTCCTGGG TCCTTATACC
TTTAGCATCT GTGACACCTC CAACTTCTCC GACTACATCC GTGGAGGCAT CGTCAGTCAG
GTCAAAGTAC CTAAGAAGAT TAGCTTTAAA TCCTTGGTGG CCTCACTGGC AGAACCTGAC
TTTGTGGTGA CGGACTTCGC CAAGTTTTCT CGCCCTGCCC AGCTGCACAT TGGCTTCCAG
GCCCTGCACC AGTTCTGTGC TCAGCATGGC CGGCCACCTC GGCCCCGCAA TGAGGAGGAT
GCAGCAGAAC TGGTAGCCTT AGCACAGGCT GTGAATGCTC GAGCCCTGCC AGCAGTGCAG
CAAAATAACC TGGACGAGGA CCTCATCCGG AAGCTGGCAT ATGTGGCTGC TGGGGATCTG
GCACCCATAA ACGCCTTCAT TGGGGGCCTG GCTGCCCAGG AAGTCATGAA GGCCTGCTCC
GGGAAGTTCA TGCCCATCAT GCAGTGGCTA TACTTTGATG CCCTTGAGTG TCTCCCTGAG
GACAAAGAGG TCCTCACAGA GGACAAGTGC CTCCAGCGCC AGAACCGTTA TGACGGGCAA
GTGGCTGTGT TTGGCTCAGA CCTGCAAGAG AAGCTGGGCA AGCAGAAGTA TTTCCTGGTG
GGTGCGGGGG CCATTGGCTG TGAGCTGCTC AAGAACTTTG CCATGATTGG GCTGGGCTGC
GGGGAGGGTG GAGAAATCAT CGTTACAGAC ATGGACACCA TTGAGAAGTC AAATCTGAAT
CGACAGTTTC TTTTCCGGCC CTGGGATGTC ACGAAGTTAA AGTCTGACAC GGCTGCTGCA
GCTGTGCGCC AAATGAATCC ACATATCCGG GTGACAAGCC ACCAGAACCG TGTGGGTCCT
GACACGGAGC GCATCTATGA TGACGATTTT TTCCAAAACC TAGATGGCGT GGCCAATGCC
CTGGACAACG TGGATGCCCG CATGTACATG GACCGCCGCT GTGTCTACTA CCGGAAGCCA
CTGCTGGAGT CAGGCACACT GGGCACCAAA GGCAATGTGC AGGTGGTGAT CCCCTTCCTG
ACAGAGTCGT ACAGTTCCAG CCAGGACCCA CCTGAGAAGT CCATCCCCAT CTGTACCCTG
AAGAACTTCC CTAATGCCAT CGAGCACACC CTGCAGTGGG CTCGGGATGA GTTTGAAGGC
CTCTTCAAGC AGCCAGCAGA AAATGTCAAC CAGTACCTCA CAGACCCCAA GTTTGTGGAG
CGAACACTGC GGCTGGCAGG CACTCAGCCC TTGGAGGTGC TGGAGGCTGT GCAGCGCAGC
CTGGTGCTGC AGCGACCACA GACCTGGGCT GACTGCGTGA CCTGGGCCTG CCACCACTGG
CACACCCAGT ACTCGAACAA CATCCGGCAG CTGCTGCACA ACTTCCCTCC TGACCAGCTC
ACAAGCTCAG GAGCGCCGTT CTGGTCTGGG CCCAAACGCT GTCCACACCC GCTCACCTTT
GATGTCAACA ATCCCCTGCA TCTGGACTAT GTGATGGCTG CTGCCAACCT GTTTGCCCAG
ACCTACGGGC TGACAGGCTC TCAGGACCGA GCTGCTGTGG CCACATTCCT GCAGTCTGTG
CAGGTCCCCG AATTCACCCC CAAGTCTGGC GTCAAGATCC ATGTTTCTGA CCAGGAGCTG
CAGAGCGCCA ATGCCTCTGT TGATGACAGT CGTCTAGAGG AGCTCAAAGC CACTCTGCCC
AGCCCAGACA AGCTCCCTGG ATTCAAGATG TACCCCATTG ACTTTGAGAA GGATGATGAC
AGCAACTTTC ATATGGATTT CATCGTGGCT GCATCCAACC TCCGGGCAGA AAACTATGAC
ATTCCTTCTG CAGACCGGCA CAAGAGCAAG CTGATTGCAG GGAAGATCAT CCCAGCCATT
GCCACGACCA CAGCAGCCGT GGTTGGCCTT GTGTGTCTGG AGCTGTACAA GGTTGTGCAG
GGGCACCGAC AGCTTGACTC CTACAAGAAT GGTTTCCTCA ACTTGGCCCT GCCTTTCTTT
GGTTTCTCTG AACCCCTTGC CGCACCACGT CACCAGTACT ATAACCAAGA GTGGACATTG
TGGGATCGCT TTGAGGTACA AGGGCTGCAG CCTAATGGTG AGGAGATGAC CCTCAAACAG
TTCCTCGACT ATTTTAAGAC AGAGCACAAA TTAGAGATCA CCATGCTGTC CCAGGGCGTG
TCCATGCTCT ATTCCTTCTT CATGCCAGCT GCCAAGCTCA AGGAACGGTT GGATCAGCCG
ATGACAGAGA TTGTGAGCCG TGTGTCGAAG CGAAAGCTGG GCCGCCACGT GCGGGCGCTG
GTGCTTGAGC TGTGCTGTAA CGACGAGAGC GGCGAGGATG TCGAGGTTCC CTATGTCCGA
TACACCATCC GCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_005272706.1 CDS 584..3778 Translation
Target ORF information:
RefSeq Version XM_005272649.1 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X5, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_005272649.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGAGATCTC TACCCCAGAT GTCCAGCTCG CCGCTGTCCA AGAAACGTCG CGTGTCCGGG
CCTGATCCAA AGCCGGGTTC TAACTGCTCC CCTGCCCAGT CCGTGTTGTC CGAAGTGCCC
TCGGTGCCAA CCAACGGAAT GGCCAAGAAC GGCAGTGAAG CAGACATAGA CGAGGGCCTT
TACTCCCGGC AGCTGTATGT GTTGGGCCAT GAGGCAATGA AGCGGCTCCA GACATCCAGT
GTCCTGGTAT CAGGCCTGCG GGGCCTGGGC GTGGAGATCG CTAAGAACAT CATCCTTGGT
GGGGTCAAGG CTGTTACCCT ACATGACCAG GGCACTGCCC AGTGGGCTGA TCTTTCCTCC
CAGTTCTACC TGCGGGAGGA GGACATCGGT AAAAACCGGG CCGAGGTATC ACAGCCCCGC
CTCGCTGAGC TCAACAGCTA TGTGCCTGTC ACTGCCTACA CTGGACCCCT CGTTGAGGAC
TTCCTTAGTG GTTTCCAGGT GGTGGTGCTC ACCAACACCC CCCTGGAGGA CCAGCTGCGA
GTGGGTGAGT TCTGTCACAA CCGTGGCATC AAGCTGGTGG TGGCAGACAC GCGGGGCCTG
TTTGGGCAGC TCTTCTGTGA CTTTGGAGAG GAAATGATCC TCACAGATTC CAATGGGGAG
CAGCCACTCA GTGCTATGGT TTCTATGGTT ACCAAGGACA ACCCCGGTGT GGTTACCTGC
CTGGATGAGG CCCGACACGG GTTTGAGAGC GGGGACTTTG TCTCCTTTTC AGAAGTACAG
GGCATGGTTG AACTCAACGG AAATCAGCCC ATGGAGATCA AAGTCCTGGG TCCTTATACC
TTTAGCATCT GTGACACCTC CAACTTCTCC GACTACATCC GTGGAGGCAT CGTCAGTCAG
GTCAAAGTAC CTAAGAAGAT TAGCTTTAAA TCCTTGGTGG CCTCACTGGC AGAACCTGAC
TTTGTGGTGA CGGACTTCGC CAAGTTTTCT CGCCCTGCCC AGCTGCACAT TGGCTTCCAG
GCCCTGCACC AGTTCTGTGC TCAGCATGGC CGGCCACCTC GGCCCCGCAA TGAGGAGGAT
GCAGCAGAAC TGGTAGCCTT AGCACAGGCT GTGAATGCTC GAGCCCTGCC AGCAGTGCAG
CAAAATAACC TGGACGAGGA CCTCATCCGG AAGCTGGCAT ATGTGGCTGC TGGGGATCTG
GCACCCATAA ACGCCTTCAT TGGGGGCCTG GCTGCCCAGG AAGTCATGAA GGCCTGCTCC
GGGAAGTTCA TGCCCATCAT GCAGTGGCTA TACTTTGATG CCCTTGAGTG TCTCCCTGAG
GACAAAGAGG TCCTCACAGA GGACAAGTGC CTCCAGCGCC AGAACCGTTA TGACGGGCAA
GTGGCTGTGT TTGGCTCAGA CCTGCAAGAG AAGCTGGGCA AGCAGAAGTA TTTCCTGGTG
GGTGCGGGGG CCATTGGCTG TGAGCTGCTC AAGAACTTTG CCATGATTGG GCTGGGCTGC
GGGGAGGGTG GAGAAATCAT CGTTACAGAC ATGGACACCA TTGAGAAGTC AAATCTGAAT
CGACAGTTTC TTTTCCGGCC CTGGGATGTC ACGAAGTTAA AGTCTGACAC GGCTGCTGCA
GCTGTGCGCC AAATGAATCC ACATATCCGG GTGACAAGCC ACCAGAACCG TGTGGGTCCT
GACACGGAGC GCATCTATGA TGACGATTTT TTCCAAAACC TAGATGGCGT GGCCAATGCC
CTGGACAACG TGGATGCCCG CATGTACATG GACCGCCGCT GTGTCTACTA CCGGAAGCCA
CTGCTGGAGT CAGGCACACT GGGCACCAAA GGCAATGTGC AGGTGGTGAT CCCCTTCCTG
ACAGAGTCGT ACAGTTCCAG CCAGGACCCA CCTGAGAAGT CCATCCCCAT CTGTACCCTG
AAGAACTTCC CTAATGCCAT CGAGCACACC CTGCAGTGGG CTCGGGATGA GTTTGAAGGC
CTCTTCAAGC AGCCAGCAGA AAATGTCAAC CAGTACCTCA CAGACCCCAA GTTTGTGGAG
CGAACACTGC GGCTGGCAGG CACTCAGCCC TTGGAGGTGC TGGAGGCTGT GCAGCGCAGC
CTGGTGCTGC AGCGACCACA GACCTGGGCT GACTGCGTGA CCTGGGCCTG CCACCACTGG
CACACCCAGT ACTCGAACAA CATCCGGCAG CTGCTGCACA ACTTCCCTCC TGACCAGCTC
ACAAGCTCAG GAGCGCCGTT CTGGTCTGGG CCCAAACGCT GTCCACACCC GCTCACCTTT
GATGTCAACA ATCCCCTGCA TCTGGACTAT GTGATGGCTG CTGCCAACCT GTTTGCCCAG
ACCTACGGGC TGACAGGCTC TCAGGACCGA GCTGCTGTGG CCACATTCCT GCAGTCTGTG
CAGGTCCCCG AATTCACCCC CAAGTCTGGC GTCAAGATCC ATGTTTCTGA CCAGGAGCTG
CAGAGCGCCA ATGCCTCTGT TGATGACAGT CGTCTAGAGG AGCTCAAAGC CACTCTGCCC
AGCCCAGACA AGCTCCCTGG ATTCAAGATG TACCCCATTG ACTTTGAGAA GGATGATGAC
AGCAACTTTC ATATGGATTT CATCGTGGCT GCATCCAACC TCCGGGCAGA AAACTATGAC
ATTCCTTCTG CAGACCGGCA CAAGAGCAAG CTGATTGCAG GGAAGATCAT CCCAGCCATT
GCCACGACCA CAGCAGCCGT GGTTGGCCTT GTGTGTCTGG AGCTGTACAA GGTTGTGCAG
GGGCACCGAC AGCTTGACTC CTACAAGAAT GGTTTCCTCA ACTTGGCCCT GCCTTTCTTT
GGTTTCTCTG AACCCCTTGC CGCACCACGT CACCAGTACT ATAACCAAGA GTGGACATTG
TGGGATCGCT TTGAGGTACA AGGGCTGCAG CCTAATGGTG AGGAGATGAC CCTCAAACAG
TTCCTCGACT ATTTTAAGAC AGAGCACAAA TTAGAGATCA CCATGCTGTC CCAGGGCGTG
TCCATGCTCT ATTCCTTCTT CATGCCAGCT GCCAAGCTCA AGGAACGGTT GGATCAGCCG
ATGACAGAGA TTGTGAGCCG TGTGTCGAAG CGAAAGCTGG GCCGCCACGT GCGGGCGCTG
GTGCTTGAGC TGTGCTGTAA CGACGAGAGC GGCGAGGATG TCGAGGTTCC CTATGTCCGA
TACACCATCC GCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu54021 Clone ID Related Accession (Same CDS sequence) XM_017029779.2 , XM_005272649.1 , XM_017029779.1 Accession Version XM_017029779.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X4 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGAGATCTC TACCCCAGAT GTCCAGCTCG CCGCTGTCCA AGAAACGTCG CGTGTCCGGG
CCTGATCCAA AGCCGGGTTC TAACTGCTCC CCTGCCCAGT CCGTGTTGTC CGAAGTGCCC
TCGGTGCCAA CCAACGGAAT GGCCAAGAAC GGCAGTGAAG CAGACATAGA CGAGGGCCTT
TACTCCCGGC AGCTGTATGT GTTGGGCCAT GAGGCAATGA AGCGGCTCCA GACATCCAGT
GTCCTGGTAT CAGGCCTGCG GGGCCTGGGC GTGGAGATCG CTAAGAACAT CATCCTTGGT
GGGGTCAAGG CTGTTACCCT ACATGACCAG GGCACTGCCC AGTGGGCTGA TCTTTCCTCC
CAGTTCTACC TGCGGGAGGA GGACATCGGT AAAAACCGGG CCGAGGTATC ACAGCCCCGC
CTCGCTGAGC TCAACAGCTA TGTGCCTGTC ACTGCCTACA CTGGACCCCT CGTTGAGGAC
TTCCTTAGTG GTTTCCAGGT GGTGGTGCTC ACCAACACCC CCCTGGAGGA CCAGCTGCGA
GTGGGTGAGT TCTGTCACAA CCGTGGCATC AAGCTGGTGG TGGCAGACAC GCGGGGCCTG
TTTGGGCAGC TCTTCTGTGA CTTTGGAGAG GAAATGATCC TCACAGATTC CAATGGGGAG
CAGCCACTCA GTGCTATGGT TTCTATGGTT ACCAAGGACA ACCCCGGTGT GGTTACCTGC
CTGGATGAGG CCCGACACGG GTTTGAGAGC GGGGACTTTG TCTCCTTTTC AGAAGTACAG
GGCATGGTTG AACTCAACGG AAATCAGCCC ATGGAGATCA AAGTCCTGGG TCCTTATACC
TTTAGCATCT GTGACACCTC CAACTTCTCC GACTACATCC GTGGAGGCAT CGTCAGTCAG
GTCAAAGTAC CTAAGAAGAT TAGCTTTAAA TCCTTGGTGG CCTCACTGGC AGAACCTGAC
TTTGTGGTGA CGGACTTCGC CAAGTTTTCT CGCCCTGCCC AGCTGCACAT TGGCTTCCAG
GCCCTGCACC AGTTCTGTGC TCAGCATGGC CGGCCACCTC GGCCCCGCAA TGAGGAGGAT
GCAGCAGAAC TGGTAGCCTT AGCACAGGCT GTGAATGCTC GAGCCCTGCC AGCAGTGCAG
CAAAATAACC TGGACGAGGA CCTCATCCGG AAGCTGGCAT ATGTGGCTGC TGGGGATCTG
GCACCCATAA ACGCCTTCAT TGGGGGCCTG GCTGCCCAGG AAGTCATGAA GGCCTGCTCC
GGGAAGTTCA TGCCCATCAT GCAGTGGCTA TACTTTGATG CCCTTGAGTG TCTCCCTGAG
GACAAAGAGG TCCTCACAGA GGACAAGTGC CTCCAGCGCC AGAACCGTTA TGACGGGCAA
GTGGCTGTGT TTGGCTCAGA CCTGCAAGAG AAGCTGGGCA AGCAGAAGTA TTTCCTGGTG
GGTGCGGGGG CCATTGGCTG TGAGCTGCTC AAGAACTTTG CCATGATTGG GCTGGGCTGC
GGGGAGGGTG GAGAAATCAT CGTTACAGAC ATGGACACCA TTGAGAAGTC AAATCTGAAT
CGACAGTTTC TTTTCCGGCC CTGGGATGTC ACGAAGTTAA AGTCTGACAC GGCTGCTGCA
GCTGTGCGCC AAATGAATCC ACATATCCGG GTGACAAGCC ACCAGAACCG TGTGGGTCCT
GACACGGAGC GCATCTATGA TGACGATTTT TTCCAAAACC TAGATGGCGT GGCCAATGCC
CTGGACAACG TGGATGCCCG CATGTACATG GACCGCCGCT GTGTCTACTA CCGGAAGCCA
CTGCTGGAGT CAGGCACACT GGGCACCAAA GGCAATGTGC AGGTGGTGAT CCCCTTCCTG
ACAGAGTCGT ACAGTTCCAG CCAGGACCCA CCTGAGAAGT CCATCCCCAT CTGTACCCTG
AAGAACTTCC CTAATGCCAT CGAGCACACC CTGCAGTGGG CTCGGGATGA GTTTGAAGGC
CTCTTCAAGC AGCCAGCAGA AAATGTCAAC CAGTACCTCA CAGACCCCAA GTTTGTGGAG
CGAACACTGC GGCTGGCAGG CACTCAGCCC TTGGAGGTGC TGGAGGCTGT GCAGCGCAGC
CTGGTGCTGC AGCGACCACA GACCTGGGCT GACTGCGTGA CCTGGGCCTG CCACCACTGG
CACACCCAGT ACTCGAACAA CATCCGGCAG CTGCTGCACA ACTTCCCTCC TGACCAGCTC
ACAAGCTCAG GAGCGCCGTT CTGGTCTGGG CCCAAACGCT GTCCACACCC GCTCACCTTT
GATGTCAACA ATCCCCTGCA TCTGGACTAT GTGATGGCTG CTGCCAACCT GTTTGCCCAG
ACCTACGGGC TGACAGGCTC TCAGGACCGA GCTGCTGTGG CCACATTCCT GCAGTCTGTG
CAGGTCCCCG AATTCACCCC CAAGTCTGGC GTCAAGATCC ATGTTTCTGA CCAGGAGCTG
CAGAGCGCCA ATGCCTCTGT TGATGACAGT CGTCTAGAGG AGCTCAAAGC CACTCTGCCC
AGCCCAGACA AGCTCCCTGG ATTCAAGATG TACCCCATTG ACTTTGAGAA GGATGATGAC
AGCAACTTTC ATATGGATTT CATCGTGGCT GCATCCAACC TCCGGGCAGA AAACTATGAC
ATTCCTTCTG CAGACCGGCA CAAGAGCAAG CTGATTGCAG GGAAGATCAT CCCAGCCATT
GCCACGACCA CAGCAGCCGT GGTTGGCCTT GTGTGTCTGG AGCTGTACAA GGTTGTGCAG
GGGCACCGAC AGCTTGACTC CTACAAGAAT GGTTTCCTCA ACTTGGCCCT GCCTTTCTTT
GGTTTCTCTG AACCCCTTGC CGCACCACGT CACCAGTACT ATAACCAAGA GTGGACATTG
TGGGATCGCT TTGAGGTACA AGGGCTGCAG CCTAATGGTG AGGAGATGAC CCTCAAACAG
TTCCTCGACT ATTTTAAGAC AGAGCACAAA TTAGAGATCA CCATGCTGTC CCAGGGCGTG
TCCATGCTCT ATTCCTTCTT CATGCCAGCT GCCAAGCTCA AGGAACGGTT GGATCAGCCG
ATGACAGAGA TTGTGAGCCG TGTGTCGAAG CGAAAGCTGG GCCGCCACGT GCGGGCGCTG
GTGCTTGAGC TGTGCTGTAA CGACGAGAGC GGCGAGGATG TCGAGGTTCC CTATGTCCGA
TACACCATCC GCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016885268.1 CDS 2983..6177 Translation
Target ORF information:
RefSeq Version XM_017029779.1 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017029779.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGAGATCTC TACCCCAGAT GTCCAGCTCG CCGCTGTCCA AGAAACGTCG CGTGTCCGGG
CCTGATCCAA AGCCGGGTTC TAACTGCTCC CCTGCCCAGT CCGTGTTGTC CGAAGTGCCC
TCGGTGCCAA CCAACGGAAT GGCCAAGAAC GGCAGTGAAG CAGACATAGA CGAGGGCCTT
TACTCCCGGC AGCTGTATGT GTTGGGCCAT GAGGCAATGA AGCGGCTCCA GACATCCAGT
GTCCTGGTAT CAGGCCTGCG GGGCCTGGGC GTGGAGATCG CTAAGAACAT CATCCTTGGT
GGGGTCAAGG CTGTTACCCT ACATGACCAG GGCACTGCCC AGTGGGCTGA TCTTTCCTCC
CAGTTCTACC TGCGGGAGGA GGACATCGGT AAAAACCGGG CCGAGGTATC ACAGCCCCGC
CTCGCTGAGC TCAACAGCTA TGTGCCTGTC ACTGCCTACA CTGGACCCCT CGTTGAGGAC
TTCCTTAGTG GTTTCCAGGT GGTGGTGCTC ACCAACACCC CCCTGGAGGA CCAGCTGCGA
GTGGGTGAGT TCTGTCACAA CCGTGGCATC AAGCTGGTGG TGGCAGACAC GCGGGGCCTG
TTTGGGCAGC TCTTCTGTGA CTTTGGAGAG GAAATGATCC TCACAGATTC CAATGGGGAG
CAGCCACTCA GTGCTATGGT TTCTATGGTT ACCAAGGACA ACCCCGGTGT GGTTACCTGC
CTGGATGAGG CCCGACACGG GTTTGAGAGC GGGGACTTTG TCTCCTTTTC AGAAGTACAG
GGCATGGTTG AACTCAACGG AAATCAGCCC ATGGAGATCA AAGTCCTGGG TCCTTATACC
TTTAGCATCT GTGACACCTC CAACTTCTCC GACTACATCC GTGGAGGCAT CGTCAGTCAG
GTCAAAGTAC CTAAGAAGAT TAGCTTTAAA TCCTTGGTGG CCTCACTGGC AGAACCTGAC
TTTGTGGTGA CGGACTTCGC CAAGTTTTCT CGCCCTGCCC AGCTGCACAT TGGCTTCCAG
GCCCTGCACC AGTTCTGTGC TCAGCATGGC CGGCCACCTC GGCCCCGCAA TGAGGAGGAT
GCAGCAGAAC TGGTAGCCTT AGCACAGGCT GTGAATGCTC GAGCCCTGCC AGCAGTGCAG
CAAAATAACC TGGACGAGGA CCTCATCCGG AAGCTGGCAT ATGTGGCTGC TGGGGATCTG
GCACCCATAA ACGCCTTCAT TGGGGGCCTG GCTGCCCAGG AAGTCATGAA GGCCTGCTCC
GGGAAGTTCA TGCCCATCAT GCAGTGGCTA TACTTTGATG CCCTTGAGTG TCTCCCTGAG
GACAAAGAGG TCCTCACAGA GGACAAGTGC CTCCAGCGCC AGAACCGTTA TGACGGGCAA
GTGGCTGTGT TTGGCTCAGA CCTGCAAGAG AAGCTGGGCA AGCAGAAGTA TTTCCTGGTG
GGTGCGGGGG CCATTGGCTG TGAGCTGCTC AAGAACTTTG CCATGATTGG GCTGGGCTGC
GGGGAGGGTG GAGAAATCAT CGTTACAGAC ATGGACACCA TTGAGAAGTC AAATCTGAAT
CGACAGTTTC TTTTCCGGCC CTGGGATGTC ACGAAGTTAA AGTCTGACAC GGCTGCTGCA
GCTGTGCGCC AAATGAATCC ACATATCCGG GTGACAAGCC ACCAGAACCG TGTGGGTCCT
GACACGGAGC GCATCTATGA TGACGATTTT TTCCAAAACC TAGATGGCGT GGCCAATGCC
CTGGACAACG TGGATGCCCG CATGTACATG GACCGCCGCT GTGTCTACTA CCGGAAGCCA
CTGCTGGAGT CAGGCACACT GGGCACCAAA GGCAATGTGC AGGTGGTGAT CCCCTTCCTG
ACAGAGTCGT ACAGTTCCAG CCAGGACCCA CCTGAGAAGT CCATCCCCAT CTGTACCCTG
AAGAACTTCC CTAATGCCAT CGAGCACACC CTGCAGTGGG CTCGGGATGA GTTTGAAGGC
CTCTTCAAGC AGCCAGCAGA AAATGTCAAC CAGTACCTCA CAGACCCCAA GTTTGTGGAG
CGAACACTGC GGCTGGCAGG CACTCAGCCC TTGGAGGTGC TGGAGGCTGT GCAGCGCAGC
CTGGTGCTGC AGCGACCACA GACCTGGGCT GACTGCGTGA CCTGGGCCTG CCACCACTGG
CACACCCAGT ACTCGAACAA CATCCGGCAG CTGCTGCACA ACTTCCCTCC TGACCAGCTC
ACAAGCTCAG GAGCGCCGTT CTGGTCTGGG CCCAAACGCT GTCCACACCC GCTCACCTTT
GATGTCAACA ATCCCCTGCA TCTGGACTAT GTGATGGCTG CTGCCAACCT GTTTGCCCAG
ACCTACGGGC TGACAGGCTC TCAGGACCGA GCTGCTGTGG CCACATTCCT GCAGTCTGTG
CAGGTCCCCG AATTCACCCC CAAGTCTGGC GTCAAGATCC ATGTTTCTGA CCAGGAGCTG
CAGAGCGCCA ATGCCTCTGT TGATGACAGT CGTCTAGAGG AGCTCAAAGC CACTCTGCCC
AGCCCAGACA AGCTCCCTGG ATTCAAGATG TACCCCATTG ACTTTGAGAA GGATGATGAC
AGCAACTTTC ATATGGATTT CATCGTGGCT GCATCCAACC TCCGGGCAGA AAACTATGAC
ATTCCTTCTG CAGACCGGCA CAAGAGCAAG CTGATTGCAG GGAAGATCAT CCCAGCCATT
GCCACGACCA CAGCAGCCGT GGTTGGCCTT GTGTGTCTGG AGCTGTACAA GGTTGTGCAG
GGGCACCGAC AGCTTGACTC CTACAAGAAT GGTTTCCTCA ACTTGGCCCT GCCTTTCTTT
GGTTTCTCTG AACCCCTTGC CGCACCACGT CACCAGTACT ATAACCAAGA GTGGACATTG
TGGGATCGCT TTGAGGTACA AGGGCTGCAG CCTAATGGTG AGGAGATGAC CCTCAAACAG
TTCCTCGACT ATTTTAAGAC AGAGCACAAA TTAGAGATCA CCATGCTGTC CCAGGGCGTG
TCCATGCTCT ATTCCTTCTT CATGCCAGCT GCCAAGCTCA AGGAACGGTT GGATCAGCCG
ATGACAGAGA TTGTGAGCCG TGTGTCGAAG CGAAAGCTGG GCCGCCACGT GCGGGCGCTG
GTGCTTGAGC TGTGCTGTAA CGACGAGAGC GGCGAGGATG TCGAGGTTCC CTATGTCCGA
TACACCATCC GCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu61201 Clone ID Related Accession (Same CDS sequence) XM_017029778.2 , XM_017029778.1 Accession Version XM_017029778.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X2 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241ATGCTTACGC TTGAAAATCT TCAGTTTGGA GACTCAGGTT CTATGCTCTG TGTTATTTAT
CATCTCGATA ACCCCGTGGG GAAGATGTCC AGCTCGCCGC TGTCCAAGAA ACGTCGCGTG
TCCGGGCCTG ATCCAAAGCC GGGTTCTAAC TGCTCCCCTG CCCAGTCCGT GTTGTCCGAA
GTGCCCTCGG TGCCAACCAA CGGAATGGCC AAGAACGGCA GTGAAGCAGA CATAGACGAG
GGCCTTTACT CCCGGCAGCT GTATGTGTTG GGCCATGAGG CAATGAAGCG GCTCCAGACA
TCCAGTGTCC TGGTATCAGG CCTGCGGGGC CTGGGCGTGG AGATCGCTAA GAACATCATC
CTTGGTGGGG TCAAGGCTGT TACCCTACAT GACCAGGGCA CTGCCCAGTG GGCTGATCTT
TCCTCCCAGT TCTACCTGCG GGAGGAGGAC ATCGGTAAAA ACCGGGCCGA GGTATCACAG
CCCCGCCTCG CTGAGCTCAA CAGCTATGTG CCTGTCACTG CCTACACTGG ACCCCTCGTT
GAGGACTTCC TTAGTGGTTT CCAGGTGGTG GTGCTCACCA ACACCCCCCT GGAGGACCAG
CTGCGAGTGG GTGAGTTCTG TCACAACCGT GGCATCAAGC TGGTGGTGGC AGACACGCGG
GGCCTGTTTG GGCAGCTCTT CTGTGACTTT GGAGAGGAAA TGATCCTCAC AGATTCCAAT
GGGGAGCAGC CACTCAGTGC TATGGTTTCT ATGGTTACCA AGGACAACCC CGGTGTGGTT
ACCTGCCTGG ATGAGGCCCG ACACGGGTTT GAGAGCGGGG ACTTTGTCTC CTTTTCAGAA
GTACAGGGCA TGGTTGAACT CAACGGAAAT CAGCCCATGG AGATCAAAGT CCTGGGTCCT
TATACCTTTA GCATCTGTGA CACCTCCAAC TTCTCCGACT ACATCCGTGG AGGCATCGTC
AGTCAGGTCA AAGTACCTAA GAAGATTAGC TTTAAATCCT TGGTGGCCTC ACTGGCAGAA
CCTGACTTTG TGGTGACGGA CTTCGCCAAG TTTTCTCGCC CTGCCCAGCT GCACATTGGC
TTCCAGGCCC TGCACCAGTT CTGTGCTCAG CATGGCCGGC CACCTCGGCC CCGCAATGAG
GAGGATGCAG CAGAACTGGT AGCCTTAGCA CAGGCTGTGA ATGCTCGAGC CCTGCCAGCA
GTGCAGCAAA ATAACCTGGA CGAGGACCTC ATCCGGAAGC TGGCATATGT GGCTGCTGGG
GATCTGGCAC CCATAAACGC CTTCATTGGG GGCCTGGCTG CCCAGGAAGT CATGAAGGCC
TGCTCCGGGA AGTTCATGCC CATCATGCAG TGGCTATACT TTGATGCCCT TGAGTGTCTC
CCTGAGGACA AAGAGGTCCT CACAGAGGAC AAGTGCCTCC AGCGCCAGAA CCGTTATGAC
GGGCAAGTGG CTGTGTTTGG CTCAGACCTG CAAGAGAAGC TGGGCAAGCA GAAGTATTTC
CTGGTGGGTG CGGGGGCCAT TGGCTGTGAG CTGCTCAAGA ACTTTGCCAT GATTGGGCTG
GGCTGCGGGG AGGGTGGAGA AATCATCGTT ACAGACATGG ACACCATTGA GAAGTCAAAT
CTGAATCGAC AGTTTCTTTT CCGGCCCTGG GATGTCACGA AGTTAAAGTC TGACACGGCT
GCTGCAGCTG TGCGCCAAAT GAATCCACAT ATCCGGGTGA CAAGCCACCA GAACCGTGTG
GGTCCTGACA CGGAGCGCAT CTATGATGAC GATTTTTTCC AAAACCTAGA TGGCGTGGCC
AATGCCCTGG ACAACGTGGA TGCCCGCATG TACATGGACC GCCGCTGTGT CTACTACCGG
AAGCCACTGC TGGAGTCAGG CACACTGGGC ACCAAAGGCA ATGTGCAGGT GGTGATCCCC
TTCCTGACAG AGTCGTACAG TTCCAGCCAG GACCCACCTG AGAAGTCCAT CCCCATCTGT
ACCCTGAAGA ACTTCCCTAA TGCCATCGAG CACACCCTGC AGTGGGCTCG GGATGAGTTT
GAAGGCCTCT TCAAGCAGCC AGCAGAAAAT GTCAACCAGT ACCTCACAGA CCCCAAGTTT
GTGGAGCGAA CACTGCGGCT GGCAGGCACT CAGCCCTTGG AGGTGCTGGA GGCTGTGCAG
CGCAGCCTGG TGCTGCAGCG ACCACAGACC TGGGCTGACT GCGTGACCTG GGCCTGCCAC
CACTGGCACA CCCAGTACTC GAACAACATC CGGCAGCTGC TGCACAACTT CCCTCCTGAC
CAGCTCACAA GCTCAGGAGC GCCGTTCTGG TCTGGGCCCA AACGCTGTCC ACACCCGCTC
ACCTTTGATG TCAACAATCC CCTGCATCTG GACTATGTGA TGGCTGCTGC CAACCTGTTT
GCCCAGACCT ACGGGCTGAC AGGCTCTCAG GACCGAGCTG CTGTGGCCAC ATTCCTGCAG
TCTGTGCAGG TCCCCGAATT CACCCCCAAG TCTGGCGTCA AGATCCATGT TTCTGACCAG
GAGCTGCAGA GCGCCAATGC CTCTGTTGAT GACAGTCGTC TAGAGGAGCT CAAAGCCACT
CTGCCCAGCC CAGACAAGCT CCCTGGATTC AAGATGTACC CCATTGACTT TGAGAAGGAT
GATGACAGCA ACTTTCATAT GGATTTCATC GTGGCTGCAT CCAACCTCCG GGCAGAAAAC
TATGACATTC CTTCTGCAGA CCGGCACAAG AGCAAGCTGA TTGCAGGGAA GATCATCCCA
GCCATTGCCA CGACCACAGC AGCCGTGGTT GGCCTTGTGT GTCTGGAGCT GTACAAGGTT
GTGCAGGGGC ACCGACAGCT TGACTCCTAC AAGAATGGTT TCCTCAACTT GGCCCTGCCT
TTCTTTGGTT TCTCTGAACC CCTTGCCGCA CCACGTCACC AGTACTATAA CCAAGAGTGG
ACATTGTGGG ATCGCTTTGA GGTACAAGGG CTGCAGCCTA ATGGTGAGGA GATGACCCTC
AAACAGTTCC TCGACTATTT TAAGACAGAG CACAAATTAG AGATCACCAT GCTGTCCCAG
GGCGTGTCCA TGCTCTATTC CTTCTTCATG CCAGCTGCCA AGCTCAAGGA ACGGTTGGAT
CAGCCGATGA CAGAGATTGT GAGCCGTGTG TCGAAGCGAA AGCTGGGCCG CCACGTGCGG
GCGCTGGTGC TTGAGCTGTG CTGTAACGAC GAGAGCGGCG AGGATGTCGA GGTTCCCTAT
GTCCGATACA CCATCCGCTG AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016885267.1 CDS 2340..5600 Translation
Target ORF information:
RefSeq Version XM_017029778.2 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017029778.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241ATGCTTACGC TTGAAAATCT TCAGTTTGGA GACTCAGGTT CTATGCTCTG TGTTATTTAT
CATCTCGATA ACCCCGTGGG GAAGATGTCC AGCTCGCCGC TGTCCAAGAA ACGTCGCGTG
TCCGGGCCTG ATCCAAAGCC GGGTTCTAAC TGCTCCCCTG CCCAGTCCGT GTTGTCCGAA
GTGCCCTCGG TGCCAACCAA CGGAATGGCC AAGAACGGCA GTGAAGCAGA CATAGACGAG
GGCCTTTACT CCCGGCAGCT GTATGTGTTG GGCCATGAGG CAATGAAGCG GCTCCAGACA
TCCAGTGTCC TGGTATCAGG CCTGCGGGGC CTGGGCGTGG AGATCGCTAA GAACATCATC
CTTGGTGGGG TCAAGGCTGT TACCCTACAT GACCAGGGCA CTGCCCAGTG GGCTGATCTT
TCCTCCCAGT TCTACCTGCG GGAGGAGGAC ATCGGTAAAA ACCGGGCCGA GGTATCACAG
CCCCGCCTCG CTGAGCTCAA CAGCTATGTG CCTGTCACTG CCTACACTGG ACCCCTCGTT
GAGGACTTCC TTAGTGGTTT CCAGGTGGTG GTGCTCACCA ACACCCCCCT GGAGGACCAG
CTGCGAGTGG GTGAGTTCTG TCACAACCGT GGCATCAAGC TGGTGGTGGC AGACACGCGG
GGCCTGTTTG GGCAGCTCTT CTGTGACTTT GGAGAGGAAA TGATCCTCAC AGATTCCAAT
GGGGAGCAGC CACTCAGTGC TATGGTTTCT ATGGTTACCA AGGACAACCC CGGTGTGGTT
ACCTGCCTGG ATGAGGCCCG ACACGGGTTT GAGAGCGGGG ACTTTGTCTC CTTTTCAGAA
GTACAGGGCA TGGTTGAACT CAACGGAAAT CAGCCCATGG AGATCAAAGT CCTGGGTCCT
TATACCTTTA GCATCTGTGA CACCTCCAAC TTCTCCGACT ACATCCGTGG AGGCATCGTC
AGTCAGGTCA AAGTACCTAA GAAGATTAGC TTTAAATCCT TGGTGGCCTC ACTGGCAGAA
CCTGACTTTG TGGTGACGGA CTTCGCCAAG TTTTCTCGCC CTGCCCAGCT GCACATTGGC
TTCCAGGCCC TGCACCAGTT CTGTGCTCAG CATGGCCGGC CACCTCGGCC CCGCAATGAG
GAGGATGCAG CAGAACTGGT AGCCTTAGCA CAGGCTGTGA ATGCTCGAGC CCTGCCAGCA
GTGCAGCAAA ATAACCTGGA CGAGGACCTC ATCCGGAAGC TGGCATATGT GGCTGCTGGG
GATCTGGCAC CCATAAACGC CTTCATTGGG GGCCTGGCTG CCCAGGAAGT CATGAAGGCC
TGCTCCGGGA AGTTCATGCC CATCATGCAG TGGCTATACT TTGATGCCCT TGAGTGTCTC
CCTGAGGACA AAGAGGTCCT CACAGAGGAC AAGTGCCTCC AGCGCCAGAA CCGTTATGAC
GGGCAAGTGG CTGTGTTTGG CTCAGACCTG CAAGAGAAGC TGGGCAAGCA GAAGTATTTC
CTGGTGGGTG CGGGGGCCAT TGGCTGTGAG CTGCTCAAGA ACTTTGCCAT GATTGGGCTG
GGCTGCGGGG AGGGTGGAGA AATCATCGTT ACAGACATGG ACACCATTGA GAAGTCAAAT
CTGAATCGAC AGTTTCTTTT CCGGCCCTGG GATGTCACGA AGTTAAAGTC TGACACGGCT
GCTGCAGCTG TGCGCCAAAT GAATCCACAT ATCCGGGTGA CAAGCCACCA GAACCGTGTG
GGTCCTGACA CGGAGCGCAT CTATGATGAC GATTTTTTCC AAAACCTAGA TGGCGTGGCC
AATGCCCTGG ACAACGTGGA TGCCCGCATG TACATGGACC GCCGCTGTGT CTACTACCGG
AAGCCACTGC TGGAGTCAGG CACACTGGGC ACCAAAGGCA ATGTGCAGGT GGTGATCCCC
TTCCTGACAG AGTCGTACAG TTCCAGCCAG GACCCACCTG AGAAGTCCAT CCCCATCTGT
ACCCTGAAGA ACTTCCCTAA TGCCATCGAG CACACCCTGC AGTGGGCTCG GGATGAGTTT
GAAGGCCTCT TCAAGCAGCC AGCAGAAAAT GTCAACCAGT ACCTCACAGA CCCCAAGTTT
GTGGAGCGAA CACTGCGGCT GGCAGGCACT CAGCCCTTGG AGGTGCTGGA GGCTGTGCAG
CGCAGCCTGG TGCTGCAGCG ACCACAGACC TGGGCTGACT GCGTGACCTG GGCCTGCCAC
CACTGGCACA CCCAGTACTC GAACAACATC CGGCAGCTGC TGCACAACTT CCCTCCTGAC
CAGCTCACAA GCTCAGGAGC GCCGTTCTGG TCTGGGCCCA AACGCTGTCC ACACCCGCTC
ACCTTTGATG TCAACAATCC CCTGCATCTG GACTATGTGA TGGCTGCTGC CAACCTGTTT
GCCCAGACCT ACGGGCTGAC AGGCTCTCAG GACCGAGCTG CTGTGGCCAC ATTCCTGCAG
TCTGTGCAGG TCCCCGAATT CACCCCCAAG TCTGGCGTCA AGATCCATGT TTCTGACCAG
GAGCTGCAGA GCGCCAATGC CTCTGTTGAT GACAGTCGTC TAGAGGAGCT CAAAGCCACT
CTGCCCAGCC CAGACAAGCT CCCTGGATTC AAGATGTACC CCATTGACTT TGAGAAGGAT
GATGACAGCA ACTTTCATAT GGATTTCATC GTGGCTGCAT CCAACCTCCG GGCAGAAAAC
TATGACATTC CTTCTGCAGA CCGGCACAAG AGCAAGCTGA TTGCAGGGAA GATCATCCCA
GCCATTGCCA CGACCACAGC AGCCGTGGTT GGCCTTGTGT GTCTGGAGCT GTACAAGGTT
GTGCAGGGGC ACCGACAGCT TGACTCCTAC AAGAATGGTT TCCTCAACTT GGCCCTGCCT
TTCTTTGGTT TCTCTGAACC CCTTGCCGCA CCACGTCACC AGTACTATAA CCAAGAGTGG
ACATTGTGGG ATCGCTTTGA GGTACAAGGG CTGCAGCCTA ATGGTGAGGA GATGACCCTC
AAACAGTTCC TCGACTATTT TAAGACAGAG CACAAATTAG AGATCACCAT GCTGTCCCAG
GGCGTGTCCA TGCTCTATTC CTTCTTCATG CCAGCTGCCA AGCTCAAGGA ACGGTTGGAT
CAGCCGATGA CAGAGATTGT GAGCCGTGTG TCGAAGCGAA AGCTGGGCCG CCACGTGCGG
GCGCTGGTGC TTGAGCTGTG CTGTAACGAC GAGAGCGGCG AGGATGTCGA GGTTCCCTAT
GTCCGATACA CCATCCGCTG AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu61201 Clone ID Related Accession (Same CDS sequence) XM_017029778.2 , XM_017029778.1 Accession Version XM_017029778.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X2 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241ATGCTTACGC TTGAAAATCT TCAGTTTGGA GACTCAGGTT CTATGCTCTG TGTTATTTAT
CATCTCGATA ACCCCGTGGG GAAGATGTCC AGCTCGCCGC TGTCCAAGAA ACGTCGCGTG
TCCGGGCCTG ATCCAAAGCC GGGTTCTAAC TGCTCCCCTG CCCAGTCCGT GTTGTCCGAA
GTGCCCTCGG TGCCAACCAA CGGAATGGCC AAGAACGGCA GTGAAGCAGA CATAGACGAG
GGCCTTTACT CCCGGCAGCT GTATGTGTTG GGCCATGAGG CAATGAAGCG GCTCCAGACA
TCCAGTGTCC TGGTATCAGG CCTGCGGGGC CTGGGCGTGG AGATCGCTAA GAACATCATC
CTTGGTGGGG TCAAGGCTGT TACCCTACAT GACCAGGGCA CTGCCCAGTG GGCTGATCTT
TCCTCCCAGT TCTACCTGCG GGAGGAGGAC ATCGGTAAAA ACCGGGCCGA GGTATCACAG
CCCCGCCTCG CTGAGCTCAA CAGCTATGTG CCTGTCACTG CCTACACTGG ACCCCTCGTT
GAGGACTTCC TTAGTGGTTT CCAGGTGGTG GTGCTCACCA ACACCCCCCT GGAGGACCAG
CTGCGAGTGG GTGAGTTCTG TCACAACCGT GGCATCAAGC TGGTGGTGGC AGACACGCGG
GGCCTGTTTG GGCAGCTCTT CTGTGACTTT GGAGAGGAAA TGATCCTCAC AGATTCCAAT
GGGGAGCAGC CACTCAGTGC TATGGTTTCT ATGGTTACCA AGGACAACCC CGGTGTGGTT
ACCTGCCTGG ATGAGGCCCG ACACGGGTTT GAGAGCGGGG ACTTTGTCTC CTTTTCAGAA
GTACAGGGCA TGGTTGAACT CAACGGAAAT CAGCCCATGG AGATCAAAGT CCTGGGTCCT
TATACCTTTA GCATCTGTGA CACCTCCAAC TTCTCCGACT ACATCCGTGG AGGCATCGTC
AGTCAGGTCA AAGTACCTAA GAAGATTAGC TTTAAATCCT TGGTGGCCTC ACTGGCAGAA
CCTGACTTTG TGGTGACGGA CTTCGCCAAG TTTTCTCGCC CTGCCCAGCT GCACATTGGC
TTCCAGGCCC TGCACCAGTT CTGTGCTCAG CATGGCCGGC CACCTCGGCC CCGCAATGAG
GAGGATGCAG CAGAACTGGT AGCCTTAGCA CAGGCTGTGA ATGCTCGAGC CCTGCCAGCA
GTGCAGCAAA ATAACCTGGA CGAGGACCTC ATCCGGAAGC TGGCATATGT GGCTGCTGGG
GATCTGGCAC CCATAAACGC CTTCATTGGG GGCCTGGCTG CCCAGGAAGT CATGAAGGCC
TGCTCCGGGA AGTTCATGCC CATCATGCAG TGGCTATACT TTGATGCCCT TGAGTGTCTC
CCTGAGGACA AAGAGGTCCT CACAGAGGAC AAGTGCCTCC AGCGCCAGAA CCGTTATGAC
GGGCAAGTGG CTGTGTTTGG CTCAGACCTG CAAGAGAAGC TGGGCAAGCA GAAGTATTTC
CTGGTGGGTG CGGGGGCCAT TGGCTGTGAG CTGCTCAAGA ACTTTGCCAT GATTGGGCTG
GGCTGCGGGG AGGGTGGAGA AATCATCGTT ACAGACATGG ACACCATTGA GAAGTCAAAT
CTGAATCGAC AGTTTCTTTT CCGGCCCTGG GATGTCACGA AGTTAAAGTC TGACACGGCT
GCTGCAGCTG TGCGCCAAAT GAATCCACAT ATCCGGGTGA CAAGCCACCA GAACCGTGTG
GGTCCTGACA CGGAGCGCAT CTATGATGAC GATTTTTTCC AAAACCTAGA TGGCGTGGCC
AATGCCCTGG ACAACGTGGA TGCCCGCATG TACATGGACC GCCGCTGTGT CTACTACCGG
AAGCCACTGC TGGAGTCAGG CACACTGGGC ACCAAAGGCA ATGTGCAGGT GGTGATCCCC
TTCCTGACAG AGTCGTACAG TTCCAGCCAG GACCCACCTG AGAAGTCCAT CCCCATCTGT
ACCCTGAAGA ACTTCCCTAA TGCCATCGAG CACACCCTGC AGTGGGCTCG GGATGAGTTT
GAAGGCCTCT TCAAGCAGCC AGCAGAAAAT GTCAACCAGT ACCTCACAGA CCCCAAGTTT
GTGGAGCGAA CACTGCGGCT GGCAGGCACT CAGCCCTTGG AGGTGCTGGA GGCTGTGCAG
CGCAGCCTGG TGCTGCAGCG ACCACAGACC TGGGCTGACT GCGTGACCTG GGCCTGCCAC
CACTGGCACA CCCAGTACTC GAACAACATC CGGCAGCTGC TGCACAACTT CCCTCCTGAC
CAGCTCACAA GCTCAGGAGC GCCGTTCTGG TCTGGGCCCA AACGCTGTCC ACACCCGCTC
ACCTTTGATG TCAACAATCC CCTGCATCTG GACTATGTGA TGGCTGCTGC CAACCTGTTT
GCCCAGACCT ACGGGCTGAC AGGCTCTCAG GACCGAGCTG CTGTGGCCAC ATTCCTGCAG
TCTGTGCAGG TCCCCGAATT CACCCCCAAG TCTGGCGTCA AGATCCATGT TTCTGACCAG
GAGCTGCAGA GCGCCAATGC CTCTGTTGAT GACAGTCGTC TAGAGGAGCT CAAAGCCACT
CTGCCCAGCC CAGACAAGCT CCCTGGATTC AAGATGTACC CCATTGACTT TGAGAAGGAT
GATGACAGCA ACTTTCATAT GGATTTCATC GTGGCTGCAT CCAACCTCCG GGCAGAAAAC
TATGACATTC CTTCTGCAGA CCGGCACAAG AGCAAGCTGA TTGCAGGGAA GATCATCCCA
GCCATTGCCA CGACCACAGC AGCCGTGGTT GGCCTTGTGT GTCTGGAGCT GTACAAGGTT
GTGCAGGGGC ACCGACAGCT TGACTCCTAC AAGAATGGTT TCCTCAACTT GGCCCTGCCT
TTCTTTGGTT TCTCTGAACC CCTTGCCGCA CCACGTCACC AGTACTATAA CCAAGAGTGG
ACATTGTGGG ATCGCTTTGA GGTACAAGGG CTGCAGCCTA ATGGTGAGGA GATGACCCTC
AAACAGTTCC TCGACTATTT TAAGACAGAG CACAAATTAG AGATCACCAT GCTGTCCCAG
GGCGTGTCCA TGCTCTATTC CTTCTTCATG CCAGCTGCCA AGCTCAAGGA ACGGTTGGAT
CAGCCGATGA CAGAGATTGT GAGCCGTGTG TCGAAGCGAA AGCTGGGCCG CCACGTGCGG
GCGCTGGTGC TTGAGCTGTG CTGTAACGAC GAGAGCGGCG AGGATGTCGA GGTTCCCTAT
GTCCGATACA CCATCCGCTG AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016885267.1 CDS 2409..5669 Translation
Target ORF information:
RefSeq Version XM_017029778.1 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017029778.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241ATGCTTACGC TTGAAAATCT TCAGTTTGGA GACTCAGGTT CTATGCTCTG TGTTATTTAT
CATCTCGATA ACCCCGTGGG GAAGATGTCC AGCTCGCCGC TGTCCAAGAA ACGTCGCGTG
TCCGGGCCTG ATCCAAAGCC GGGTTCTAAC TGCTCCCCTG CCCAGTCCGT GTTGTCCGAA
GTGCCCTCGG TGCCAACCAA CGGAATGGCC AAGAACGGCA GTGAAGCAGA CATAGACGAG
GGCCTTTACT CCCGGCAGCT GTATGTGTTG GGCCATGAGG CAATGAAGCG GCTCCAGACA
TCCAGTGTCC TGGTATCAGG CCTGCGGGGC CTGGGCGTGG AGATCGCTAA GAACATCATC
CTTGGTGGGG TCAAGGCTGT TACCCTACAT GACCAGGGCA CTGCCCAGTG GGCTGATCTT
TCCTCCCAGT TCTACCTGCG GGAGGAGGAC ATCGGTAAAA ACCGGGCCGA GGTATCACAG
CCCCGCCTCG CTGAGCTCAA CAGCTATGTG CCTGTCACTG CCTACACTGG ACCCCTCGTT
GAGGACTTCC TTAGTGGTTT CCAGGTGGTG GTGCTCACCA ACACCCCCCT GGAGGACCAG
CTGCGAGTGG GTGAGTTCTG TCACAACCGT GGCATCAAGC TGGTGGTGGC AGACACGCGG
GGCCTGTTTG GGCAGCTCTT CTGTGACTTT GGAGAGGAAA TGATCCTCAC AGATTCCAAT
GGGGAGCAGC CACTCAGTGC TATGGTTTCT ATGGTTACCA AGGACAACCC CGGTGTGGTT
ACCTGCCTGG ATGAGGCCCG ACACGGGTTT GAGAGCGGGG ACTTTGTCTC CTTTTCAGAA
GTACAGGGCA TGGTTGAACT CAACGGAAAT CAGCCCATGG AGATCAAAGT CCTGGGTCCT
TATACCTTTA GCATCTGTGA CACCTCCAAC TTCTCCGACT ACATCCGTGG AGGCATCGTC
AGTCAGGTCA AAGTACCTAA GAAGATTAGC TTTAAATCCT TGGTGGCCTC ACTGGCAGAA
CCTGACTTTG TGGTGACGGA CTTCGCCAAG TTTTCTCGCC CTGCCCAGCT GCACATTGGC
TTCCAGGCCC TGCACCAGTT CTGTGCTCAG CATGGCCGGC CACCTCGGCC CCGCAATGAG
GAGGATGCAG CAGAACTGGT AGCCTTAGCA CAGGCTGTGA ATGCTCGAGC CCTGCCAGCA
GTGCAGCAAA ATAACCTGGA CGAGGACCTC ATCCGGAAGC TGGCATATGT GGCTGCTGGG
GATCTGGCAC CCATAAACGC CTTCATTGGG GGCCTGGCTG CCCAGGAAGT CATGAAGGCC
TGCTCCGGGA AGTTCATGCC CATCATGCAG TGGCTATACT TTGATGCCCT TGAGTGTCTC
CCTGAGGACA AAGAGGTCCT CACAGAGGAC AAGTGCCTCC AGCGCCAGAA CCGTTATGAC
GGGCAAGTGG CTGTGTTTGG CTCAGACCTG CAAGAGAAGC TGGGCAAGCA GAAGTATTTC
CTGGTGGGTG CGGGGGCCAT TGGCTGTGAG CTGCTCAAGA ACTTTGCCAT GATTGGGCTG
GGCTGCGGGG AGGGTGGAGA AATCATCGTT ACAGACATGG ACACCATTGA GAAGTCAAAT
CTGAATCGAC AGTTTCTTTT CCGGCCCTGG GATGTCACGA AGTTAAAGTC TGACACGGCT
GCTGCAGCTG TGCGCCAAAT GAATCCACAT ATCCGGGTGA CAAGCCACCA GAACCGTGTG
GGTCCTGACA CGGAGCGCAT CTATGATGAC GATTTTTTCC AAAACCTAGA TGGCGTGGCC
AATGCCCTGG ACAACGTGGA TGCCCGCATG TACATGGACC GCCGCTGTGT CTACTACCGG
AAGCCACTGC TGGAGTCAGG CACACTGGGC ACCAAAGGCA ATGTGCAGGT GGTGATCCCC
TTCCTGACAG AGTCGTACAG TTCCAGCCAG GACCCACCTG AGAAGTCCAT CCCCATCTGT
ACCCTGAAGA ACTTCCCTAA TGCCATCGAG CACACCCTGC AGTGGGCTCG GGATGAGTTT
GAAGGCCTCT TCAAGCAGCC AGCAGAAAAT GTCAACCAGT ACCTCACAGA CCCCAAGTTT
GTGGAGCGAA CACTGCGGCT GGCAGGCACT CAGCCCTTGG AGGTGCTGGA GGCTGTGCAG
CGCAGCCTGG TGCTGCAGCG ACCACAGACC TGGGCTGACT GCGTGACCTG GGCCTGCCAC
CACTGGCACA CCCAGTACTC GAACAACATC CGGCAGCTGC TGCACAACTT CCCTCCTGAC
CAGCTCACAA GCTCAGGAGC GCCGTTCTGG TCTGGGCCCA AACGCTGTCC ACACCCGCTC
ACCTTTGATG TCAACAATCC CCTGCATCTG GACTATGTGA TGGCTGCTGC CAACCTGTTT
GCCCAGACCT ACGGGCTGAC AGGCTCTCAG GACCGAGCTG CTGTGGCCAC ATTCCTGCAG
TCTGTGCAGG TCCCCGAATT CACCCCCAAG TCTGGCGTCA AGATCCATGT TTCTGACCAG
GAGCTGCAGA GCGCCAATGC CTCTGTTGAT GACAGTCGTC TAGAGGAGCT CAAAGCCACT
CTGCCCAGCC CAGACAAGCT CCCTGGATTC AAGATGTACC CCATTGACTT TGAGAAGGAT
GATGACAGCA ACTTTCATAT GGATTTCATC GTGGCTGCAT CCAACCTCCG GGCAGAAAAC
TATGACATTC CTTCTGCAGA CCGGCACAAG AGCAAGCTGA TTGCAGGGAA GATCATCCCA
GCCATTGCCA CGACCACAGC AGCCGTGGTT GGCCTTGTGT GTCTGGAGCT GTACAAGGTT
GTGCAGGGGC ACCGACAGCT TGACTCCTAC AAGAATGGTT TCCTCAACTT GGCCCTGCCT
TTCTTTGGTT TCTCTGAACC CCTTGCCGCA CCACGTCACC AGTACTATAA CCAAGAGTGG
ACATTGTGGG ATCGCTTTGA GGTACAAGGG CTGCAGCCTA ATGGTGAGGA GATGACCCTC
AAACAGTTCC TCGACTATTT TAAGACAGAG CACAAATTAG AGATCACCAT GCTGTCCCAG
GGCGTGTCCA TGCTCTATTC CTTCTTCATG CCAGCTGCCA AGCTCAAGGA ACGGTTGGAT
CAGCCGATGA CAGAGATTGT GAGCCGTGTG TCGAAGCGAA AGCTGGGCCG CCACGTGCGG
GCGCTGGTGC TTGAGCTGTG CTGTAACGAC GAGAGCGGCG AGGATGTCGA GGTTCCCTAT
GTCCGATACA CCATCCGCTG AThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu61202 Clone ID Related Accession (Same CDS sequence) XM_011543954.2 Accession Version XM_011543954.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X3 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGCTCTGTG TTATTTATCA TCTCGATAAC CCCGTGGGGA AGATGTCCAG CTCGCCGCTG
TCCAAGAAAC GTCGCGTGTC CGGGCCTGAT CCAAAGCCGG GTTCTAACTG CTCCCCTGCC
CAGTCCGTGT TGTCCGAAGT GCCCTCGGTG CCAACCAACG GAATGGCCAA GAACGGCAGT
GAAGCAGACA TAGACGAGGG CCTTTACTCC CGGCAGCTGT ATGTGTTGGG CCATGAGGCA
ATGAAGCGGC TCCAGACATC CAGTGTCCTG GTATCAGGCC TGCGGGGCCT GGGCGTGGAG
ATCGCTAAGA ACATCATCCT TGGTGGGGTC AAGGCTGTTA CCCTACATGA CCAGGGCACT
GCCCAGTGGG CTGATCTTTC CTCCCAGTTC TACCTGCGGG AGGAGGACAT CGGTAAAAAC
CGGGCCGAGG TATCACAGCC CCGCCTCGCT GAGCTCAACA GCTATGTGCC TGTCACTGCC
TACACTGGAC CCCTCGTTGA GGACTTCCTT AGTGGTTTCC AGGTGGTGGT GCTCACCAAC
ACCCCCCTGG AGGACCAGCT GCGAGTGGGT GAGTTCTGTC ACAACCGTGG CATCAAGCTG
GTGGTGGCAG ACACGCGGGG CCTGTTTGGG CAGCTCTTCT GTGACTTTGG AGAGGAAATG
ATCCTCACAG ATTCCAATGG GGAGCAGCCA CTCAGTGCTA TGGTTTCTAT GGTTACCAAG
GACAACCCCG GTGTGGTTAC CTGCCTGGAT GAGGCCCGAC ACGGGTTTGA GAGCGGGGAC
TTTGTCTCCT TTTCAGAAGT ACAGGGCATG GTTGAACTCA ACGGAAATCA GCCCATGGAG
ATCAAAGTCC TGGGTCCTTA TACCTTTAGC ATCTGTGACA CCTCCAACTT CTCCGACTAC
ATCCGTGGAG GCATCGTCAG TCAGGTCAAA GTACCTAAGA AGATTAGCTT TAAATCCTTG
GTGGCCTCAC TGGCAGAACC TGACTTTGTG GTGACGGACT TCGCCAAGTT TTCTCGCCCT
GCCCAGCTGC ACATTGGCTT CCAGGCCCTG CACCAGTTCT GTGCTCAGCA TGGCCGGCCA
CCTCGGCCCC GCAATGAGGA GGATGCAGCA GAACTGGTAG CCTTAGCACA GGCTGTGAAT
GCTCGAGCCC TGCCAGCAGT GCAGCAAAAT AACCTGGACG AGGACCTCAT CCGGAAGCTG
GCATATGTGG CTGCTGGGGA TCTGGCACCC ATAAACGCCT TCATTGGGGG CCTGGCTGCC
CAGGAAGTCA TGAAGGCCTG CTCCGGGAAG TTCATGCCCA TCATGCAGTG GCTATACTTT
GATGCCCTTG AGTGTCTCCC TGAGGACAAA GAGGTCCTCA CAGAGGACAA GTGCCTCCAG
CGCCAGAACC GTTATGACGG GCAAGTGGCT GTGTTTGGCT CAGACCTGCA AGAGAAGCTG
GGCAAGCAGA AGTATTTCCT GGTGGGTGCG GGGGCCATTG GCTGTGAGCT GCTCAAGAAC
TTTGCCATGA TTGGGCTGGG CTGCGGGGAG GGTGGAGAAA TCATCGTTAC AGACATGGAC
ACCATTGAGA AGTCAAATCT GAATCGACAG TTTCTTTTCC GGCCCTGGGA TGTCACGAAG
TTAAAGTCTG ACACGGCTGC TGCAGCTGTG CGCCAAATGA ATCCACATAT CCGGGTGACA
AGCCACCAGA ACCGTGTGGG TCCTGACACG GAGCGCATCT ATGATGACGA TTTTTTCCAA
AACCTAGATG GCGTGGCCAA TGCCCTGGAC AACGTGGATG CCCGCATGTA CATGGACCGC
CGCTGTGTCT ACTACCGGAA GCCACTGCTG GAGTCAGGCA CACTGGGCAC CAAAGGCAAT
GTGCAGGTGG TGATCCCCTT CCTGACAGAG TCGTACAGTT CCAGCCAGGA CCCACCTGAG
AAGTCCATCC CCATCTGTAC CCTGAAGAAC TTCCCTAATG CCATCGAGCA CACCCTGCAG
TGGGCTCGGG ATGAGTTTGA AGGCCTCTTC AAGCAGCCAG CAGAAAATGT CAACCAGTAC
CTCACAGACC CCAAGTTTGT GGAGCGAACA CTGCGGCTGG CAGGCACTCA GCCCTTGGAG
GTGCTGGAGG CTGTGCAGCG CAGCCTGGTG CTGCAGCGAC CACAGACCTG GGCTGACTGC
GTGACCTGGG CCTGCCACCA CTGGCACACC CAGTACTCGA ACAACATCCG GCAGCTGCTG
CACAACTTCC CTCCTGACCA GCTCACAAGC TCAGGAGCGC CGTTCTGGTC TGGGCCCAAA
CGCTGTCCAC ACCCGCTCAC CTTTGATGTC AACAATCCCC TGCATCTGGA CTATGTGATG
GCTGCTGCCA ACCTGTTTGC CCAGACCTAC GGGCTGACAG GCTCTCAGGA CCGAGCTGCT
GTGGCCACAT TCCTGCAGTC TGTGCAGGTC CCCGAATTCA CCCCCAAGTC TGGCGTCAAG
ATCCATGTTT CTGACCAGGA GCTGCAGAGC GCCAATGCCT CTGTTGATGA CAGTCGTCTA
GAGGAGCTCA AAGCCACTCT GCCCAGCCCA GACAAGCTCC CTGGATTCAA GATGTACCCC
ATTGACTTTG AGAAGGATGA TGACAGCAAC TTTCATATGG ATTTCATCGT GGCTGCATCC
AACCTCCGGG CAGAAAACTA TGACATTCCT TCTGCAGACC GGCACAAGAG CAAGCTGATT
GCAGGGAAGA TCATCCCAGC CATTGCCACG ACCACAGCAG CCGTGGTTGG CCTTGTGTGT
CTGGAGCTGT ACAAGGTTGT GCAGGGGCAC CGACAGCTTG ACTCCTACAA GAATGGTTTC
CTCAACTTGG CCCTGCCTTT CTTTGGTTTC TCTGAACCCC TTGCCGCACC ACGTCACCAG
TACTATAACC AAGAGTGGAC ATTGTGGGAT CGCTTTGAGG TACAAGGGCT GCAGCCTAAT
GGTGAGGAGA TGACCCTCAA ACAGTTCCTC GACTATTTTA AGACAGAGCA CAAATTAGAG
ATCACCATGC TGTCCCAGGG CGTGTCCATG CTCTATTCCT TCTTCATGCC AGCTGCCAAG
CTCAAGGAAC GGTTGGATCA GCCGATGACA GAGATTGTGA GCCGTGTGTC GAAGCGAAAG
CTGGGCCGCC ACGTGCGGGC GCTGGTGCTT GAGCTGTGCT GTAACGACGA GAGCGGCGAG
GATGTCGAGG TTCCCTATGT CCGATACACC ATCCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_011542256.1 CDS 202..3420 Translation
Target ORF information:
RefSeq Version XM_011543954.2 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_011543954.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181ATGCTCTGTG TTATTTATCA TCTCGATAAC CCCGTGGGGA AGATGTCCAG CTCGCCGCTG
TCCAAGAAAC GTCGCGTGTC CGGGCCTGAT CCAAAGCCGG GTTCTAACTG CTCCCCTGCC
CAGTCCGTGT TGTCCGAAGT GCCCTCGGTG CCAACCAACG GAATGGCCAA GAACGGCAGT
GAAGCAGACA TAGACGAGGG CCTTTACTCC CGGCAGCTGT ATGTGTTGGG CCATGAGGCA
ATGAAGCGGC TCCAGACATC CAGTGTCCTG GTATCAGGCC TGCGGGGCCT GGGCGTGGAG
ATCGCTAAGA ACATCATCCT TGGTGGGGTC AAGGCTGTTA CCCTACATGA CCAGGGCACT
GCCCAGTGGG CTGATCTTTC CTCCCAGTTC TACCTGCGGG AGGAGGACAT CGGTAAAAAC
CGGGCCGAGG TATCACAGCC CCGCCTCGCT GAGCTCAACA GCTATGTGCC TGTCACTGCC
TACACTGGAC CCCTCGTTGA GGACTTCCTT AGTGGTTTCC AGGTGGTGGT GCTCACCAAC
ACCCCCCTGG AGGACCAGCT GCGAGTGGGT GAGTTCTGTC ACAACCGTGG CATCAAGCTG
GTGGTGGCAG ACACGCGGGG CCTGTTTGGG CAGCTCTTCT GTGACTTTGG AGAGGAAATG
ATCCTCACAG ATTCCAATGG GGAGCAGCCA CTCAGTGCTA TGGTTTCTAT GGTTACCAAG
GACAACCCCG GTGTGGTTAC CTGCCTGGAT GAGGCCCGAC ACGGGTTTGA GAGCGGGGAC
TTTGTCTCCT TTTCAGAAGT ACAGGGCATG GTTGAACTCA ACGGAAATCA GCCCATGGAG
ATCAAAGTCC TGGGTCCTTA TACCTTTAGC ATCTGTGACA CCTCCAACTT CTCCGACTAC
ATCCGTGGAG GCATCGTCAG TCAGGTCAAA GTACCTAAGA AGATTAGCTT TAAATCCTTG
GTGGCCTCAC TGGCAGAACC TGACTTTGTG GTGACGGACT TCGCCAAGTT TTCTCGCCCT
GCCCAGCTGC ACATTGGCTT CCAGGCCCTG CACCAGTTCT GTGCTCAGCA TGGCCGGCCA
CCTCGGCCCC GCAATGAGGA GGATGCAGCA GAACTGGTAG CCTTAGCACA GGCTGTGAAT
GCTCGAGCCC TGCCAGCAGT GCAGCAAAAT AACCTGGACG AGGACCTCAT CCGGAAGCTG
GCATATGTGG CTGCTGGGGA TCTGGCACCC ATAAACGCCT TCATTGGGGG CCTGGCTGCC
CAGGAAGTCA TGAAGGCCTG CTCCGGGAAG TTCATGCCCA TCATGCAGTG GCTATACTTT
GATGCCCTTG AGTGTCTCCC TGAGGACAAA GAGGTCCTCA CAGAGGACAA GTGCCTCCAG
CGCCAGAACC GTTATGACGG GCAAGTGGCT GTGTTTGGCT CAGACCTGCA AGAGAAGCTG
GGCAAGCAGA AGTATTTCCT GGTGGGTGCG GGGGCCATTG GCTGTGAGCT GCTCAAGAAC
TTTGCCATGA TTGGGCTGGG CTGCGGGGAG GGTGGAGAAA TCATCGTTAC AGACATGGAC
ACCATTGAGA AGTCAAATCT GAATCGACAG TTTCTTTTCC GGCCCTGGGA TGTCACGAAG
TTAAAGTCTG ACACGGCTGC TGCAGCTGTG CGCCAAATGA ATCCACATAT CCGGGTGACA
AGCCACCAGA ACCGTGTGGG TCCTGACACG GAGCGCATCT ATGATGACGA TTTTTTCCAA
AACCTAGATG GCGTGGCCAA TGCCCTGGAC AACGTGGATG CCCGCATGTA CATGGACCGC
CGCTGTGTCT ACTACCGGAA GCCACTGCTG GAGTCAGGCA CACTGGGCAC CAAAGGCAAT
GTGCAGGTGG TGATCCCCTT CCTGACAGAG TCGTACAGTT CCAGCCAGGA CCCACCTGAG
AAGTCCATCC CCATCTGTAC CCTGAAGAAC TTCCCTAATG CCATCGAGCA CACCCTGCAG
TGGGCTCGGG ATGAGTTTGA AGGCCTCTTC AAGCAGCCAG CAGAAAATGT CAACCAGTAC
CTCACAGACC CCAAGTTTGT GGAGCGAACA CTGCGGCTGG CAGGCACTCA GCCCTTGGAG
GTGCTGGAGG CTGTGCAGCG CAGCCTGGTG CTGCAGCGAC CACAGACCTG GGCTGACTGC
GTGACCTGGG CCTGCCACCA CTGGCACACC CAGTACTCGA ACAACATCCG GCAGCTGCTG
CACAACTTCC CTCCTGACCA GCTCACAAGC TCAGGAGCGC CGTTCTGGTC TGGGCCCAAA
CGCTGTCCAC ACCCGCTCAC CTTTGATGTC AACAATCCCC TGCATCTGGA CTATGTGATG
GCTGCTGCCA ACCTGTTTGC CCAGACCTAC GGGCTGACAG GCTCTCAGGA CCGAGCTGCT
GTGGCCACAT TCCTGCAGTC TGTGCAGGTC CCCGAATTCA CCCCCAAGTC TGGCGTCAAG
ATCCATGTTT CTGACCAGGA GCTGCAGAGC GCCAATGCCT CTGTTGATGA CAGTCGTCTA
GAGGAGCTCA AAGCCACTCT GCCCAGCCCA GACAAGCTCC CTGGATTCAA GATGTACCCC
ATTGACTTTG AGAAGGATGA TGACAGCAAC TTTCATATGG ATTTCATCGT GGCTGCATCC
AACCTCCGGG CAGAAAACTA TGACATTCCT TCTGCAGACC GGCACAAGAG CAAGCTGATT
GCAGGGAAGA TCATCCCAGC CATTGCCACG ACCACAGCAG CCGTGGTTGG CCTTGTGTGT
CTGGAGCTGT ACAAGGTTGT GCAGGGGCAC CGACAGCTTG ACTCCTACAA GAATGGTTTC
CTCAACTTGG CCCTGCCTTT CTTTGGTTTC TCTGAACCCC TTGCCGCACC ACGTCACCAG
TACTATAACC AAGAGTGGAC ATTGTGGGAT CGCTTTGAGG TACAAGGGCT GCAGCCTAAT
GGTGAGGAGA TGACCCTCAA ACAGTTCCTC GACTATTTTA AGACAGAGCA CAAATTAGAG
ATCACCATGC TGTCCCAGGG CGTGTCCATG CTCTATTCCT TCTTCATGCC AGCTGCCAAG
CTCAAGGAAC GGTTGGATCA GCCGATGACA GAGATTGTGA GCCGTGTGTC GAAGCGAAAG
CTGGGCCGCC ACGTGCGGGC GCTGGTGCTT GAGCTGTGCT GTAACGACGA GAGCGGCGAG
GATGTCGAGG TTCCCTATGT CCGATACACC ATCCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu99446 Clone ID Related Accession (Same CDS sequence) XM_017029777.1 Accession Version XM_017029777.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301ATGTCCAAGC CTCACTTCCC TCCTGCACTC CCCGCTCCCG CCGCCATCCC TGGGATTAAG
GGAGAGGAGA ATGGCTGCTT GTTACTGCCT ACCTACTCTG TGCCAGGTTC TATGCTCTGT
GTTATTTATC ATCTCGATAA CCCCGTGGGG AAGATGTCCA GCTCGCCGCT GTCCAAGAAA
CGTCGCGTGT CCGGGCCTGA TCCAAAGCCG GGTTCTAACT GCTCCCCTGC CCAGTCCGTG
TTGTCCGAAG TGCCCTCGGT GCCAACCAAC GGAATGGCCA AGAACGGCAG TGAAGCAGAC
ATAGACGAGG GCCTTTACTC CCGGCAGCTG TATGTGTTGG GCCATGAGGC AATGAAGCGG
CTCCAGACAT CCAGTGTCCT GGTATCAGGC CTGCGGGGCC TGGGCGTGGA GATCGCTAAG
AACATCATCC TTGGTGGGGT CAAGGCTGTT ACCCTACATG ACCAGGGCAC TGCCCAGTGG
GCTGATCTTT CCTCCCAGTT CTACCTGCGG GAGGAGGACA TCGGTAAAAA CCGGGCCGAG
GTATCACAGC CCCGCCTCGC TGAGCTCAAC AGCTATGTGC CTGTCACTGC CTACACTGGA
CCCCTCGTTG AGGACTTCCT TAGTGGTTTC CAGGTGGTGG TGCTCACCAA CACCCCCCTG
GAGGACCAGC TGCGAGTGGG TGAGTTCTGT CACAACCGTG GCATCAAGCT GGTGGTGGCA
GACACGCGGG GCCTGTTTGG GCAGCTCTTC TGTGACTTTG GAGAGGAAAT GATCCTCACA
GATTCCAATG GGGAGCAGCC ACTCAGTGCT ATGGTTTCTA TGGTTACCAA GGACAACCCC
GGTGTGGTTA CCTGCCTGGA TGAGGCCCGA CACGGGTTTG AGAGCGGGGA CTTTGTCTCC
TTTTCAGAAG TACAGGGCAT GGTTGAACTC AACGGAAATC AGCCCATGGA GATCAAAGTC
CTGGGTCCTT ATACCTTTAG CATCTGTGAC ACCTCCAACT TCTCCGACTA CATCCGTGGA
GGCATCGTCA GTCAGGTCAA AGTACCTAAG AAGATTAGCT TTAAATCCTT GGTGGCCTCA
CTGGCAGAAC CTGACTTTGT GGTGACGGAC TTCGCCAAGT TTTCTCGCCC TGCCCAGCTG
CACATTGGCT TCCAGGCCCT GCACCAGTTC TGTGCTCAGC ATGGCCGGCC ACCTCGGCCC
CGCAATGAGG AGGATGCAGC AGAACTGGTA GCCTTAGCAC AGGCTGTGAA TGCTCGAGCC
CTGCCAGCAG TGCAGCAAAA TAACCTGGAC GAGGACCTCA TCCGGAAGCT GGCATATGTG
GCTGCTGGGG ATCTGGCACC CATAAACGCC TTCATTGGGG GCCTGGCTGC CCAGGAAGTC
ATGAAGGCCT GCTCCGGGAA GTTCATGCCC ATCATGCAGT GGCTATACTT TGATGCCCTT
GAGTGTCTCC CTGAGGACAA AGAGGTCCTC ACAGAGGACA AGTGCCTCCA GCGCCAGAAC
CGTTATGACG GGCAAGTGGC TGTGTTTGGC TCAGACCTGC AAGAGAAGCT GGGCAAGCAG
AAGTATTTCC TGGTGGGTGC GGGGGCCATT GGCTGTGAGC TGCTCAAGAA CTTTGCCATG
ATTGGGCTGG GCTGCGGGGA GGGTGGAGAA ATCATCGTTA CAGACATGGA CACCATTGAG
AAGTCAAATC TGAATCGACA GTTTCTTTTC CGGCCCTGGG ATGTCACGAA GTTAAAGTCT
GACACGGCTG CTGCAGCTGT GCGCCAAATG AATCCACATA TCCGGGTGAC AAGCCACCAG
AACCGTGTGG GTCCTGACAC GGAGCGCATC TATGATGACG ATTTTTTCCA AAACCTAGAT
GGCGTGGCCA ATGCCCTGGA CAACGTGGAT GCCCGCATGT ACATGGACCG CCGCTGTGTC
TACTACCGGA AGCCACTGCT GGAGTCAGGC ACACTGGGCA CCAAAGGCAA TGTGCAGGTG
GTGATCCCCT TCCTGACAGA GTCGTACAGT TCCAGCCAGG ACCCACCTGA GAAGTCCATC
CCCATCTGTA CCCTGAAGAA CTTCCCTAAT GCCATCGAGC ACACCCTGCA GTGGGCTCGG
GATGAGTTTG AAGGCCTCTT CAAGCAGCCA GCAGAAAATG TCAACCAGTA CCTCACAGAC
CCCAAGTTTG TGGAGCGAAC ACTGCGGCTG GCAGGCACTC AGCCCTTGGA GGTGCTGGAG
GCTGTGCAGC GCAGCCTGGT GCTGCAGCGA CCACAGACCT GGGCTGACTG CGTGACCTGG
GCCTGCCACC ACTGGCACAC CCAGTACTCG AACAACATCC GGCAGCTGCT GCACAACTTC
CCTCCTGACC AGCTCACAAG CTCAGGAGCG CCGTTCTGGT CTGGGCCCAA ACGCTGTCCA
CACCCGCTCA CCTTTGATGT CAACAATCCC CTGCATCTGG ACTATGTGAT GGCTGCTGCC
AACCTGTTTG CCCAGACCTA CGGGCTGACA GGCTCTCAGG ACCGAGCTGC TGTGGCCACA
TTCCTGCAGT CTGTGCAGGT CCCCGAATTC ACCCCCAAGT CTGGCGTCAA GATCCATGTT
TCTGACCAGG AGCTGCAGAG CGCCAATGCC TCTGTTGATG ACAGTCGTCT AGAGGAGCTC
AAAGCCACTC TGCCCAGCCC AGACAAGCTC CCTGGATTCA AGATGTACCC CATTGACTTT
GAGAAGGATG ATGACAGCAA CTTTCATATG GATTTCATCG TGGCTGCATC CAACCTCCGG
GCAGAAAACT ATGACATTCC TTCTGCAGAC CGGCACAAGA GCAAGCTGAT TGCAGGGAAG
ATCATCCCAG CCATTGCCAC GACCACAGCA GCCGTGGTTG GCCTTGTGTG TCTGGAGCTG
TACAAGGTTG TGCAGGGGCA CCGACAGCTT GACTCCTACA AGAATGGTTT CCTCAACTTG
GCCCTGCCTT TCTTTGGTTT CTCTGAACCC CTTGCCGCAC CACGTCACCA GTACTATAAC
CAAGAGTGGA CATTGTGGGA TCGCTTTGAG GTACAAGGGC TGCAGCCTAA TGGTGAGGAG
ATGACCCTCA AACAGTTCCT CGACTATTTT AAGACAGAGC ACAAATTAGA GATCACCATG
CTGTCCCAGG GCGTGTCCAT GCTCTATTCC TTCTTCATGC CAGCTGCCAA GCTCAAGGAA
CGGTTGGATC AGCCGATGAC AGAGATTGTG AGCCGTGTGT CGAAGCGAAA GCTGGGCCGC
CACGTGCGGG CGCTGGTGCT TGAGCTGTGC TGTAACGACG AGAGCGGCGA GGATGTCGAG
GTTCCCTATG TCCGATACAC CATCCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016885266.1 CDS 1166..4495 Translation
Target ORF information:
RefSeq Version XM_017029777.1 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017029777.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301ATGTCCAAGC CTCACTTCCC TCCTGCACTC CCCGCTCCCG CCGCCATCCC TGGGATTAAG
GGAGAGGAGA ATGGCTGCTT GTTACTGCCT ACCTACTCTG TGCCAGGTTC TATGCTCTGT
GTTATTTATC ATCTCGATAA CCCCGTGGGG AAGATGTCCA GCTCGCCGCT GTCCAAGAAA
CGTCGCGTGT CCGGGCCTGA TCCAAAGCCG GGTTCTAACT GCTCCCCTGC CCAGTCCGTG
TTGTCCGAAG TGCCCTCGGT GCCAACCAAC GGAATGGCCA AGAACGGCAG TGAAGCAGAC
ATAGACGAGG GCCTTTACTC CCGGCAGCTG TATGTGTTGG GCCATGAGGC AATGAAGCGG
CTCCAGACAT CCAGTGTCCT GGTATCAGGC CTGCGGGGCC TGGGCGTGGA GATCGCTAAG
AACATCATCC TTGGTGGGGT CAAGGCTGTT ACCCTACATG ACCAGGGCAC TGCCCAGTGG
GCTGATCTTT CCTCCCAGTT CTACCTGCGG GAGGAGGACA TCGGTAAAAA CCGGGCCGAG
GTATCACAGC CCCGCCTCGC TGAGCTCAAC AGCTATGTGC CTGTCACTGC CTACACTGGA
CCCCTCGTTG AGGACTTCCT TAGTGGTTTC CAGGTGGTGG TGCTCACCAA CACCCCCCTG
GAGGACCAGC TGCGAGTGGG TGAGTTCTGT CACAACCGTG GCATCAAGCT GGTGGTGGCA
GACACGCGGG GCCTGTTTGG GCAGCTCTTC TGTGACTTTG GAGAGGAAAT GATCCTCACA
GATTCCAATG GGGAGCAGCC ACTCAGTGCT ATGGTTTCTA TGGTTACCAA GGACAACCCC
GGTGTGGTTA CCTGCCTGGA TGAGGCCCGA CACGGGTTTG AGAGCGGGGA CTTTGTCTCC
TTTTCAGAAG TACAGGGCAT GGTTGAACTC AACGGAAATC AGCCCATGGA GATCAAAGTC
CTGGGTCCTT ATACCTTTAG CATCTGTGAC ACCTCCAACT TCTCCGACTA CATCCGTGGA
GGCATCGTCA GTCAGGTCAA AGTACCTAAG AAGATTAGCT TTAAATCCTT GGTGGCCTCA
CTGGCAGAAC CTGACTTTGT GGTGACGGAC TTCGCCAAGT TTTCTCGCCC TGCCCAGCTG
CACATTGGCT TCCAGGCCCT GCACCAGTTC TGTGCTCAGC ATGGCCGGCC ACCTCGGCCC
CGCAATGAGG AGGATGCAGC AGAACTGGTA GCCTTAGCAC AGGCTGTGAA TGCTCGAGCC
CTGCCAGCAG TGCAGCAAAA TAACCTGGAC GAGGACCTCA TCCGGAAGCT GGCATATGTG
GCTGCTGGGG ATCTGGCACC CATAAACGCC TTCATTGGGG GCCTGGCTGC CCAGGAAGTC
ATGAAGGCCT GCTCCGGGAA GTTCATGCCC ATCATGCAGT GGCTATACTT TGATGCCCTT
GAGTGTCTCC CTGAGGACAA AGAGGTCCTC ACAGAGGACA AGTGCCTCCA GCGCCAGAAC
CGTTATGACG GGCAAGTGGC TGTGTTTGGC TCAGACCTGC AAGAGAAGCT GGGCAAGCAG
AAGTATTTCC TGGTGGGTGC GGGGGCCATT GGCTGTGAGC TGCTCAAGAA CTTTGCCATG
ATTGGGCTGG GCTGCGGGGA GGGTGGAGAA ATCATCGTTA CAGACATGGA CACCATTGAG
AAGTCAAATC TGAATCGACA GTTTCTTTTC CGGCCCTGGG ATGTCACGAA GTTAAAGTCT
GACACGGCTG CTGCAGCTGT GCGCCAAATG AATCCACATA TCCGGGTGAC AAGCCACCAG
AACCGTGTGG GTCCTGACAC GGAGCGCATC TATGATGACG ATTTTTTCCA AAACCTAGAT
GGCGTGGCCA ATGCCCTGGA CAACGTGGAT GCCCGCATGT ACATGGACCG CCGCTGTGTC
TACTACCGGA AGCCACTGCT GGAGTCAGGC ACACTGGGCA CCAAAGGCAA TGTGCAGGTG
GTGATCCCCT TCCTGACAGA GTCGTACAGT TCCAGCCAGG ACCCACCTGA GAAGTCCATC
CCCATCTGTA CCCTGAAGAA CTTCCCTAAT GCCATCGAGC ACACCCTGCA GTGGGCTCGG
GATGAGTTTG AAGGCCTCTT CAAGCAGCCA GCAGAAAATG TCAACCAGTA CCTCACAGAC
CCCAAGTTTG TGGAGCGAAC ACTGCGGCTG GCAGGCACTC AGCCCTTGGA GGTGCTGGAG
GCTGTGCAGC GCAGCCTGGT GCTGCAGCGA CCACAGACCT GGGCTGACTG CGTGACCTGG
GCCTGCCACC ACTGGCACAC CCAGTACTCG AACAACATCC GGCAGCTGCT GCACAACTTC
CCTCCTGACC AGCTCACAAG CTCAGGAGCG CCGTTCTGGT CTGGGCCCAA ACGCTGTCCA
CACCCGCTCA CCTTTGATGT CAACAATCCC CTGCATCTGG ACTATGTGAT GGCTGCTGCC
AACCTGTTTG CCCAGACCTA CGGGCTGACA GGCTCTCAGG ACCGAGCTGC TGTGGCCACA
TTCCTGCAGT CTGTGCAGGT CCCCGAATTC ACCCCCAAGT CTGGCGTCAA GATCCATGTT
TCTGACCAGG AGCTGCAGAG CGCCAATGCC TCTGTTGATG ACAGTCGTCT AGAGGAGCTC
AAAGCCACTC TGCCCAGCCC AGACAAGCTC CCTGGATTCA AGATGTACCC CATTGACTTT
GAGAAGGATG ATGACAGCAA CTTTCATATG GATTTCATCG TGGCTGCATC CAACCTCCGG
GCAGAAAACT ATGACATTCC TTCTGCAGAC CGGCACAAGA GCAAGCTGAT TGCAGGGAAG
ATCATCCCAG CCATTGCCAC GACCACAGCA GCCGTGGTTG GCCTTGTGTG TCTGGAGCTG
TACAAGGTTG TGCAGGGGCA CCGACAGCTT GACTCCTACA AGAATGGTTT CCTCAACTTG
GCCCTGCCTT TCTTTGGTTT CTCTGAACCC CTTGCCGCAC CACGTCACCA GTACTATAAC
CAAGAGTGGA CATTGTGGGA TCGCTTTGAG GTACAAGGGC TGCAGCCTAA TGGTGAGGAG
ATGACCCTCA AACAGTTCCT CGACTATTTT AAGACAGAGC ACAAATTAGA GATCACCATG
CTGTCCCAGG GCGTGTCCAT GCTCTATTCC TTCTTCATGC CAGCTGCCAA GCTCAAGGAA
CGGTTGGATC AGCCGATGAC AGAGATTGTG AGCCGTGTGT CGAAGCGAAA GCTGGGCCGC
CACGTGCGGG CGCTGGTGCT TGAGCTGTGC TGTAACGACG AGAGCGGCGA GGATGTCGAG
GTTCCCTATG TCCGATACAC CATCCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version NM_153280.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1569513600000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG703038.1 and BC013041.2. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_153280.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013041.2, SRR1803616.125708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_695012.1 CDS 105..3281 Translation
Target ORF information:
RefSeq Version NM_153280.3 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_153280.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version NM_003334.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1571760000000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI547207.1, M58028.1 and BC013041.2. On Nov 22, 2018 this sequence version replaced NM_003334.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.128662.1, SRR1660807.76023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000335972.11/ ENSP00000338413.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_003325.2 CDS 197..3373 Translation
Target ORF information:
RefSeq Version NM_003334.4 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_003334.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version NM_003334.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1506787200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI547207.1, M58028.1 and BC013041.2. On Dec 20, 2007 this sequence version replaced NM_003334.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009900.2, SRR1803613.45478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_003325.2 CDS 224..3400 Translation
Target ORF information:
RefSeq Version NM_003334.3 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_003334.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version NM_153280.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1550332800000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG703038.1 and BC013041.2. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced NM_153280.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013041.2, SRR1660807.132013.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_695012.1 CDS 152..3328 Translation
Target ORF information:
RefSeq Version NM_153280.2 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_153280.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version XM_005272650.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1426003200000 Organism Homo sapiens(Human) Product ubiquitin-like modifier-activating enzyme 1 isoform X1 Comment
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_005272707.1 Translation
Target ORF information:
RefSeq Version XM_005272650.1 Organism Homo sapiens(Human) Definition Homo sapiens ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X5, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_005272650.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version XM_011543956.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1426003200000 Organism Homo sapiens(Human) Product ubiquitin-like modifier-activating enzyme 1 isoform X1 Comment
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_011542258.1 Translation
Target ORF information:
RefSeq Version XM_011543956.1 Organism Homo sapiens(Human) Definition Homo sapiens ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X6, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_011543956.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version XM_017029780.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X5 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016885269.1 CDS 109..3285 Translation
Target ORF information:
RefSeq Version XM_017029780.1 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X6, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017029780.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu24932 Clone ID Related Accession (Same CDS sequence) NM_153280.3 , NM_003334.4 , NM_003334.3 , XM_005272650.1 , NM_153280.2 , XM_011543956.1 , XM_017029780.1 , XM_017029781.1 Accession Version XM_017029781.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product ubiquitin-like modifier-activating enzyme 1 isoform X5 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016885270.1 CDS 157..3333 Translation
Target ORF information:
RefSeq Version XM_017029781.1 Organism Homo sapiens(human) Definition Homo sapiens ubiquitin like modifier activating enzyme 1 (UBA1), transcript variant X7, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017029781.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121ATGTCCAGCT CGCCGCTGTC CAAGAAACGT CGCGTGTCCG GGCCTGATCC AAAGCCGGGT
TCTAACTGCT CCCCTGCCCA GTCCGTGTTG TCCGAAGTGC CCTCGGTGCC AACCAACGGA
ATGGCCAAGA ACGGCAGTGA AGCAGACATA GACGAGGGCC TTTACTCCCG GCAGCTGTAT
GTGTTGGGCC ATGAGGCAAT GAAGCGGCTC CAGACATCCA GTGTCCTGGT ATCAGGCCTG
CGGGGCCTGG GCGTGGAGAT CGCTAAGAAC ATCATCCTTG GTGGGGTCAA GGCTGTTACC
CTACATGACC AGGGCACTGC CCAGTGGGCT GATCTTTCCT CCCAGTTCTA CCTGCGGGAG
GAGGACATCG GTAAAAACCG GGCCGAGGTA TCACAGCCCC GCCTCGCTGA GCTCAACAGC
TATGTGCCTG TCACTGCCTA CACTGGACCC CTCGTTGAGG ACTTCCTTAG TGGTTTCCAG
GTGGTGGTGC TCACCAACAC CCCCCTGGAG GACCAGCTGC GAGTGGGTGA GTTCTGTCAC
AACCGTGGCA TCAAGCTGGT GGTGGCAGAC ACGCGGGGCC TGTTTGGGCA GCTCTTCTGT
GACTTTGGAG AGGAAATGAT CCTCACAGAT TCCAATGGGG AGCAGCCACT CAGTGCTATG
GTTTCTATGG TTACCAAGGA CAACCCCGGT GTGGTTACCT GCCTGGATGA GGCCCGACAC
GGGTTTGAGA GCGGGGACTT TGTCTCCTTT TCAGAAGTAC AGGGCATGGT TGAACTCAAC
GGAAATCAGC CCATGGAGAT CAAAGTCCTG GGTCCTTATA CCTTTAGCAT CTGTGACACC
TCCAACTTCT CCGACTACAT CCGTGGAGGC ATCGTCAGTC AGGTCAAAGT ACCTAAGAAG
ATTAGCTTTA AATCCTTGGT GGCCTCACTG GCAGAACCTG ACTTTGTGGT GACGGACTTC
GCCAAGTTTT CTCGCCCTGC CCAGCTGCAC ATTGGCTTCC AGGCCCTGCA CCAGTTCTGT
GCTCAGCATG GCCGGCCACC TCGGCCCCGC AATGAGGAGG ATGCAGCAGA ACTGGTAGCC
TTAGCACAGG CTGTGAATGC TCGAGCCCTG CCAGCAGTGC AGCAAAATAA CCTGGACGAG
GACCTCATCC GGAAGCTGGC ATATGTGGCT GCTGGGGATC TGGCACCCAT AAACGCCTTC
ATTGGGGGCC TGGCTGCCCA GGAAGTCATG AAGGCCTGCT CCGGGAAGTT CATGCCCATC
ATGCAGTGGC TATACTTTGA TGCCCTTGAG TGTCTCCCTG AGGACAAAGA GGTCCTCACA
GAGGACAAGT GCCTCCAGCG CCAGAACCGT TATGACGGGC AAGTGGCTGT GTTTGGCTCA
GACCTGCAAG AGAAGCTGGG CAAGCAGAAG TATTTCCTGG TGGGTGCGGG GGCCATTGGC
TGTGAGCTGC TCAAGAACTT TGCCATGATT GGGCTGGGCT GCGGGGAGGG TGGAGAAATC
ATCGTTACAG ACATGGACAC CATTGAGAAG TCAAATCTGA ATCGACAGTT TCTTTTCCGG
CCCTGGGATG TCACGAAGTT AAAGTCTGAC ACGGCTGCTG CAGCTGTGCG CCAAATGAAT
CCACATATCC GGGTGACAAG CCACCAGAAC CGTGTGGGTC CTGACACGGA GCGCATCTAT
GATGACGATT TTTTCCAAAA CCTAGATGGC GTGGCCAATG CCCTGGACAA CGTGGATGCC
CGCATGTACA TGGACCGCCG CTGTGTCTAC TACCGGAAGC CACTGCTGGA GTCAGGCACA
CTGGGCACCA AAGGCAATGT GCAGGTGGTG ATCCCCTTCC TGACAGAGTC GTACAGTTCC
AGCCAGGACC CACCTGAGAA GTCCATCCCC ATCTGTACCC TGAAGAACTT CCCTAATGCC
ATCGAGCACA CCCTGCAGTG GGCTCGGGAT GAGTTTGAAG GCCTCTTCAA GCAGCCAGCA
GAAAATGTCA ACCAGTACCT CACAGACCCC AAGTTTGTGG AGCGAACACT GCGGCTGGCA
GGCACTCAGC CCTTGGAGGT GCTGGAGGCT GTGCAGCGCA GCCTGGTGCT GCAGCGACCA
CAGACCTGGG CTGACTGCGT GACCTGGGCC TGCCACCACT GGCACACCCA GTACTCGAAC
AACATCCGGC AGCTGCTGCA CAACTTCCCT CCTGACCAGC TCACAAGCTC AGGAGCGCCG
TTCTGGTCTG GGCCCAAACG CTGTCCACAC CCGCTCACCT TTGATGTCAA CAATCCCCTG
CATCTGGACT ATGTGATGGC TGCTGCCAAC CTGTTTGCCC AGACCTACGG GCTGACAGGC
TCTCAGGACC GAGCTGCTGT GGCCACATTC CTGCAGTCTG TGCAGGTCCC CGAATTCACC
CCCAAGTCTG GCGTCAAGAT CCATGTTTCT GACCAGGAGC TGCAGAGCGC CAATGCCTCT
GTTGATGACA GTCGTCTAGA GGAGCTCAAA GCCACTCTGC CCAGCCCAGA CAAGCTCCCT
GGATTCAAGA TGTACCCCAT TGACTTTGAG AAGGATGATG ACAGCAACTT TCATATGGAT
TTCATCGTGG CTGCATCCAA CCTCCGGGCA GAAAACTATG ACATTCCTTC TGCAGACCGG
CACAAGAGCA AGCTGATTGC AGGGAAGATC ATCCCAGCCA TTGCCACGAC CACAGCAGCC
GTGGTTGGCC TTGTGTGTCT GGAGCTGTAC AAGGTTGTGC AGGGGCACCG ACAGCTTGAC
TCCTACAAGA ATGGTTTCCT CAACTTGGCC CTGCCTTTCT TTGGTTTCTC TGAACCCCTT
GCCGCACCAC GTCACCAGTA CTATAACCAA GAGTGGACAT TGTGGGATCG CTTTGAGGTA
CAAGGGCTGC AGCCTAATGG TGAGGAGATG ACCCTCAAAC AGTTCCTCGA CTATTTTAAG
ACAGAGCACA AATTAGAGAT CACCATGCTG TCCCAGGGCG TGTCCATGCT CTATTCCTTC
TTCATGCCAG CTGCCAAGCT CAAGGAACGG TTGGATCAGC CGATGACAGA GATTGTGAGC
CGTGTGTCGA AGCGAAAGCT GGGCCGCCAC GTGCGGGCGC TGGTGCTTGA GCTGTGCTGT
AACGACGAGA GCGGCGAGGA TGTCGAGGTT CCCTATGTCC GATACACCAT CCGCTGAThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
-
PubMed
Crystal structure of a human ubiquitin E1-ubiquitin complex reveals conserved functional elements essential for activity.
The Journal of biological chemistry293(47)18337-18352(2018 11)
Lv Z,Williams KM,Yuan L,Atkison JH,Olsen SK
Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes.
Nature communications814286(2017 01)
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