TLE2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLE2
Entrez Gene ID	7089
Full Name	transducin like enhancer of split 2
Synonyms	ESG,ESG2,GRG2
General protein information	Preferred Names transducin like enhancer of split 2 Names transducin-like enhancer protein 2 enhancer of split groucho-like protein 2 transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) transducin-like enhancer of split 2, homolog of Drosophila E(sp1)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Disorder MIM:	601041

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144762.1	NP_001138234.1	transducin-like enhancer protein 2 isoform 3
NM_001300846.2	NP_001287775.1	transducin-like enhancer protein 2 isoform 4
XM_011528230.1	XP_011526532.1	transducin-like enhancer protein 2 isoform X1
NM_003260.4	NP_003251.2	transducin-like enhancer protein 2 isoform 1
NM_001300846.1	NP_001287775.1	transducin-like enhancer protein 2 isoform 4
NM_001144761.1	NP_001138233.1	transducin-like enhancer protein 2 isoform 2
XM_006722864.2	XP_006722927.1	transducin-like enhancer protein 2 isoform X2
NM_003260.5	NP_003251.2	transducin-like enhancer protein 2 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLE2	NP_003251.2
Canis lupus familiaris (dog)	TLE2	XP_005633143.1
Pan troglodytes (chimpanzee)	TLE2	XP_512979.3
Bos taurus (cattle)	TLE2	XP_005209071.1
Mus musculus (house mouse)	Tle2	NP_062699.1
Rattus norvegicus (Norway rat)	Tle2	NP_001034102.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Böhm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, Kölbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Cellular corepressor TLE2 inhibits replication-and-transcription- activator-mediated transactivation and lytic reactivation of Kaposi's sarcoma-associated herpesvirus.
He Z, Liu Y, Liang D, Wang Z, Robertson ES, Lan K
Journal of virology84(4)2047-62(2010 Feb)

Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression.
Arce L, Pate KT, Waterman ML
BMC cancer9159(2009 May)

Groucho/TLE/R-esp proteins associate with the nuclear matrix and repress RUNX (CBF(alpha)/AML/PEBP2(alpha)) dependent activation of tissue-specific gene transcription.
Javed A, Guo B, Hiebert S, Choi JY, Green J, Zhao SC, Osborne MA, Stifani S, Stein JL, Lian JB, van Wijnen AJ, Stein GS
Journal of cell science113 ( Pt 12)2221-31(2000 Jun)

Transducin-like Enhancer of split 2, a mammalian homologue of Drosophila Groucho, acts as a transcriptional repressor, interacts with Hairy/Enhancer of split proteins, and is expressed during neuronal development.
Grbavec D, Lo R, Liu Y, Stifani S
European journal of biochemistry258(2)339-49(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TLE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09533	NM_001144762.1 Latest version!	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu09542	NM_001144761.1 Latest version!	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu51785	XM_006722864.2 Latest version!	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu61008	XM_011528230.1 Latest version!	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu09535	NM_003260.5 Latest version!	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
OHu51784	NM_001300846.2 Latest version!	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00
View Old Accession Versions >>
OHu51784	NM_001300846.1	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00
OHu09535	NM_003260.4	Homo sapiens transducin like enhancer of split 2 (TLE2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLE2 ( NM_001144762.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138234.1 Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 3, mRNA.

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CloneID	OHu09533
Clone ID Related Accession (Same CDS sequence)	NM_001144762.1
Accession Version	NM_001144762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564761600000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK293407.1 and BC017364.2. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences compared to variant 4. This results in a shorter isoform (2) with a shorter N-terminus compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293407.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCGAC ATTATGTCAT GTATTATGAG ATGTCGTACG GGCTCAACAT TGAAATGCAT 
AAGCAGGCGG AGATTGTGAA GCGTCTGAGC GGTATCTGCG CTCAGATTAT CCCCTTCCTG 
ACCCAGGAGC ATCAGCAGCA GGTGCTCCAG GCCGTAGAAC GCGCCAAGCA GGTCACCGTG 
GGGGAGCTGA ACAGCCTCAT CGGGAGTGCA TCTCCCTCGC CCCCTGAGAG TCTCGTGGAG 
GAGGAGCGAC CGAGTGGCCC TGGTGGTGGC GGGAAGCAGA GAGCAGATGA GAAGGAGCCA 
TCAGGACCTT ATGAAAGCGA CGAAGACAAG AGTGATTACA ATCTGGTGGT GGACGAGGAC 
CAACCCTCAG AGCCCCCCAG CCCGGCTACC ACCCCCTGCG GAAAGGTACC CATCTGCATT 
CCTGCCCGTC GGGACCTGGT GGACAGTCCA GCCTCCTTGG CCTCTAGCCT TGGCTCACCG 
CTGCCTAGAG CCAAGGAGCT CATCCTGAAT GACCTTCCCG CCAGCACTCC TGCCTCCAAA 
TCCTGTGACT CCTCCCCGCC CCAGGACGCT TCCACCCCCG GGCCCAGCTC GGCCAGTCAC 
CTCTGCCAGC TTGCTGCCAA GCCAGCACCT TCCACGGACA GCGTCGCCCT GAGGAGCCCC 
CTGACTCTGT CCAGTCCCTT CACCACGTCC TTCAGCCTGG GCTCCCACAG CACTCTCAAC 
GGAGACCTCT CCGTGCCCAG CTCCTACGTC AGCCTCCACC TGTCCCCCCA GGTCAGCAGC 
TCTGTGGTGT ACGGACGCTC CCCCGTGATG GCATTTGAGT CTCATCCCCA TCTCCGAGGG 
TCATCCGTCT CTTCCTCCCT ACCCAGCATC CCTGGGGGAA AGCCGGCCTA CTCCTTCCAC 
GTGTCTGCGG ACGGGCAGAT GCAGCCGGTT CCCTTCCCCT CGGATGCACT GGTAGGCGCG 
GGCATCCCGC GGCACGCCCG GCAGCTGCAC ACGCTGGCCC ATGGCGAGGT GGTCTGCGCG 
GTCACCATCA GCGGCTCCAC ACAGCATGTG TACACGGGCG GCAAGGGCTG TGTGAAGGTG 
TGGGACGTGG GCCAGCCTGG GGCCAAGACG CCCGTGGCCC AGCTCGACTG CCTGAACCGA 
GACAACTACA TTCGTTCCTG CAAGTTGCTG CCGGATGGCC GGAGTCTGAT CGTGGGCGGT 
GAGGCCAGCA CCTTGTCCAT TTGGGACCTG GCGGCGCCCA CCCCCCGTAT CAAGGCCGAG 
CTGACTTCCT CAGCCCCAGC CTGCTACGCC CTGGCCGTCA GCCCCGACGC CAAGGTTTGC 
TTCTCCTGCT GCAGCGATGG CAACATTGTG GTCTGGGACC TGCAGAATCA GACTATGGTC 
AGGCAGTTCC AGGGCCACAC GGACGGCGCC AGCTGCATTG ATATTTCCGA TTACGGCACT 
CGGCTCTGGA CAGGGGGCCT GGACAACACG GTGCGCTGCT GGGACCTGCG GGAGGGCCGC 
CAGCTGCAGC AGCATGACTT CAGCTCCCAG ATTTTCTCCC TGGGCCACTG CCCTAACCAG 
GACTGGCTGG CGGTCGGAAT GGAGAGTAGC AACGTGGAGA TCCTGCACGT CCGCAAGCCG 
GAGAAATACC AGCTGCACCT CCACGAGAGC TGCGTGCTGT CCCTGAAGTT TGCCTCCTGC 
GGACGGTGGT TTGTGAGCAC CGGGAAGGAC AACCTGCTCA ACGCCTGGAG GACGCCGTAC 
GGGGCCAGCA TTTTCCAGTC CAAGGAGTCG TCCTCAGTCC TGAGTTGTGA CATCTCCAGA 
AATAACAAAT ACATCGTGAC AGGCTCGGGG GACAAGAAGG CCACCGTGTA TGAGGTGGTC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138234.1
CDS	207..2072
Translation	MQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	NM_001144762.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144762.1

ORF Insert Sequence:

ATGCAGCGAC ATTATGTCAT GTATTATGAG ATGTCGTACG GGCTCAACAT TGAAATGCAT 
AAGCAGGCGG AGATTGTGAA GCGTCTGAGC GGTATCTGCG CTCAGATTAT CCCCTTCCTG 
ACCCAGGAGC ATCAGCAGCA GGTGCTCCAG GCCGTAGAAC GCGCCAAGCA GGTCACCGTG 
GGGGAGCTGA ACAGCCTCAT CGGGAGTGCA TCTCCCTCGC CCCCTGAGAG TCTCGTGGAG 
GAGGAGCGAC CGAGTGGCCC TGGTGGTGGC GGGAAGCAGA GAGCAGATGA GAAGGAGCCA 
TCAGGACCTT ATGAAAGCGA CGAAGACAAG AGTGATTACA ATCTGGTGGT GGACGAGGAC 
CAACCCTCAG AGCCCCCCAG CCCGGCTACC ACCCCCTGCG GAAAGGTACC CATCTGCATT 
CCTGCCCGTC GGGACCTGGT GGACAGTCCA GCCTCCTTGG CCTCTAGCCT TGGCTCACCG 
CTGCCTAGAG CCAAGGAGCT CATCCTGAAT GACCTTCCCG CCAGCACTCC TGCCTCCAAA 
TCCTGTGACT CCTCCCCGCC CCAGGACGCT TCCACCCCCG GGCCCAGCTC GGCCAGTCAC 
CTCTGCCAGC TTGCTGCCAA GCCAGCACCT TCCACGGACA GCGTCGCCCT GAGGAGCCCC 
CTGACTCTGT CCAGTCCCTT CACCACGTCC TTCAGCCTGG GCTCCCACAG CACTCTCAAC 
GGAGACCTCT CCGTGCCCAG CTCCTACGTC AGCCTCCACC TGTCCCCCCA GGTCAGCAGC 
TCTGTGGTGT ACGGACGCTC CCCCGTGATG GCATTTGAGT CTCATCCCCA TCTCCGAGGG 
TCATCCGTCT CTTCCTCCCT ACCCAGCATC CCTGGGGGAA AGCCGGCCTA CTCCTTCCAC 
GTGTCTGCGG ACGGGCAGAT GCAGCCGGTT CCCTTCCCCT CGGATGCACT GGTAGGCGCG 
GGCATCCCGC GGCACGCCCG GCAGCTGCAC ACGCTGGCCC ATGGCGAGGT GGTCTGCGCG 
GTCACCATCA GCGGCTCCAC ACAGCATGTG TACACGGGCG GCAAGGGCTG TGTGAAGGTG 
TGGGACGTGG GCCAGCCTGG GGCCAAGACG CCCGTGGCCC AGCTCGACTG CCTGAACCGA 
GACAACTACA TTCGTTCCTG CAAGTTGCTG CCGGATGGCC GGAGTCTGAT CGTGGGCGGT 
GAGGCCAGCA CCTTGTCCAT TTGGGACCTG GCGGCGCCCA CCCCCCGTAT CAAGGCCGAG 
CTGACTTCCT CAGCCCCAGC CTGCTACGCC CTGGCCGTCA GCCCCGACGC CAAGGTTTGC 
TTCTCCTGCT GCAGCGATGG CAACATTGTG GTCTGGGACC TGCAGAATCA GACTATGGTC 
AGGCAGTTCC AGGGCCACAC GGACGGCGCC AGCTGCATTG ATATTTCCGA TTACGGCACT 
CGGCTCTGGA CAGGGGGCCT GGACAACACG GTGCGCTGCT GGGACCTGCG GGAGGGCCGC 
CAGCTGCAGC AGCATGACTT CAGCTCCCAG ATTTTCTCCC TGGGCCACTG CCCTAACCAG 
GACTGGCTGG CGGTCGGAAT GGAGAGTAGC AACGTGGAGA TCCTGCACGT CCGCAAGCCG 
GAGAAATACC AGCTGCACCT CCACGAGAGC TGCGTGCTGT CCCTGAAGTT TGCCTCCTGC 
GGACGGTGGT TTGTGAGCAC CGGGAAGGAC AACCTGCTCA ACGCCTGGAG GACGCCGTAC 
GGGGCCAGCA TTTTCCAGTC CAAGGAGTCG TCCTCAGTCC TGAGTTGTGA CATCTCCAGA 
AATAACAAAT ACATCGTGAC AGGCTCGGGG GACAAGAAGG CCACCGTGTA TGAGGTGGTC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLE2 ( NM_001144761.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138233.1 Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09542
Clone ID Related Accession (Same CDS sequence)	NM_001144761.1
Accession Version	NM_001144761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564761600000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK308137.1 and BC017364.2. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences compared to variant 4, one of which results in a frameshift. This results in a shorter isoform (2) with distinct N- and C-termini compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK308137.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAAT CACGGCTCAC TGCAACCTCC GCCTCCCAAG ATTCTCCTGC TTCAGGATTA 
CAGACCCCGC TCCAGTCCGG CCAGCCCTTC AAGTTCTCGA TCTTGGAGAT CTGCGACCGC 
ATCAAAGAAG AATTCCAGTT TCTTCAGGCT CAATACCACA GCCTCAAGCT AGAATGTGAG 
AAGCTGGCCA GCGAGAAGAC GGAAATGCAG CGACATTATG TCATGTATTA TGAGATGTCG 
TACGGGCTCA ACATTGAAAT GCATAAGCAG GCGGAGATTG TGAAGCGTCT GAGCGGTATC 
TGCGCTCAGA TTATCCCCTT CCTGACCCAG GAGCATCAGC AGCAGGTGCT CCAGGCCGTA 
GAACGCGCCA AGCAGGTCAC CGTGGGGGAG CTGAACAGCC TCATCGGGCA GCAGCAGCTC 
CAGCCGCTGT CCCACCACGC ACCCCCTGTG CCCCTCACCC CCCGCCCAGC CGGGCTGGTG 
GGCGGCAGTG CTACGGGGCT GCTTGCTCTG TCTGGAGCCC TGGCTGCCCA GGCTCAGCTG 
GCGGCGGCTG TCAAGGAGGA CCGTGCGGGC GTGGAGGCCG AGGGGTCCAG AGTGGAGAGA 
GCCCCGAGCA GGAGTGCATC TCCCTCGCCC CCTGAGAGTC TCGTGGAGGA GGAGCGACCG 
AGTGGCCCTG GTGGTGGCGG GAAGCAGAGA GCAGATGAGA AGGAGCCATC AGGACCTTAT 
GAAAGCGACG AAGACAAGAG TGATTACAAT CTGGTGGTGG ACGAGGACCA ACCCTCAGAG 
CCCCCCAGCC CGGCTACCAC CCCCTGCGGA AAGGTACCCA TCTGCATTCC TGCCCGTCGG 
GACCTGGTGG ACAGTCCAGC CTCCTTGGCC TCTAGCCTTG GCTCACCGCT GCCTAGAGCC 
AAGGAGCTCA TCCTGAATGA CCTTCCCGCC AGCACTCCTG CCTCCAAATC CTGTGACTCC 
TCCCCGCCCC AGGACGCTTC CACCCCCGGG CCCAGCTCGG CCAGTCACCT CTGCCAGCTT 
GCTGCCAAGC CAGCACCTTC CACGGACAGC GTCGCCCTGA GGAGCCCCCT GACTCTGTCC 
AGTCCCTTCA CCACGTCCTT CAGCCTGGGC TCCCACAGCA CTCTCAACGG AGACCTCTCC 
GTGCCCAGCT CCTACGTCAG CCTCCACCTG TCCCCCCAGG TCAGCAGCTC TGTGGTGTAC 
GGACGCTCCC CCGTGATGGC ATTTGAGTCT CATCCCCATC TCCGAGGGTC ATCCGTCTCT 
TCCTCCCTAC CCAGCATCCC TGGGGGAAAG CCGGCCTACT CCTTCCACGT GTCTGCGGAC 
GGGCAGATGC AGCCGGTTCC CTTCCCCTCG GATGCACTGG TAGGCGCGGG CATCCCGCGG 
CACGCCCGGC AGCTGCACAC GCTGGCCCAT GGCGAGGTGG TCTGCGCGGT CACCATCAGC 
GGCTCCACAC AGCATGTGTA CACGGGCGGC AAGGGCTGTG TGAAGGTGTG GGACGTGGGC 
CAGCCTGGGG CCAAGACGCC CGTGGCCCAG CTCGACTGCC TGAACCGAGA CAACTACATT 
CGTTCCTGCA AGTTGCTGCC GGATGGCCGG AGTCTGATCG TGGGCGGTGA GGCCAGCACC 
TTGTCCATTT GGGACCTGGC GGCGCCCACC CCCCGTATCA AGGCCGAGCT GACTTCCTCA 
GCCCCAGCCT GCTACGCCCT GGCCGTCAGC CCCGACGCCA AGGTTTGCTT CTCCTGCTGC 
AGCGATGGCA ACATTGTGGT CTGGGACCTG CAGAATCAGA CTATGGTCAG GCAGTTCCAG 
GGCCACACGG ACGGCGCCAG CTGCATTGAT ATTTCCGATT ACGGCACTCG GCTCTGGACA 
GGGGGCCTGG ACAACACGGT GCGCTGCTGG GACCTGCGGG AGGGCCGCCA GCTGCAGCAG 
CATGACTTCA GCTCCCAGAT TTTCTCCCTG GGCCACTGCC CTAACCAGGA CTGGCTGGCG 
GTCGGAATGG AGAGTAGCAA CGTGGAGATC CTGCACGTCC GCAAGCCGGA GAAATACCAG 
CTGCACCTCC ACGAGAGCTG CGTGCTGTCC CTGAAGTTTG CCTCCTGCGT CCAAGGAGTC 
GTCCTCAGTC CTGAGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138233.1
CDS	85..2205
Translation	MVQSRLTATSASQDSPASGLQTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCVQGVVLSPEL

Target ORF information:

RefSeq Version	NM_001144761.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144761.1

ORF Insert Sequence:

ATGGTGCAAT CACGGCTCAC TGCAACCTCC GCCTCCCAAG ATTCTCCTGC TTCAGGATTA 
CAGACCCCGC TCCAGTCCGG CCAGCCCTTC AAGTTCTCGA TCTTGGAGAT CTGCGACCGC 
ATCAAAGAAG AATTCCAGTT TCTTCAGGCT CAATACCACA GCCTCAAGCT AGAATGTGAG 
AAGCTGGCCA GCGAGAAGAC GGAAATGCAG CGACATTATG TCATGTATTA TGAGATGTCG 
TACGGGCTCA ACATTGAAAT GCATAAGCAG GCGGAGATTG TGAAGCGTCT GAGCGGTATC 
TGCGCTCAGA TTATCCCCTT CCTGACCCAG GAGCATCAGC AGCAGGTGCT CCAGGCCGTA 
GAACGCGCCA AGCAGGTCAC CGTGGGGGAG CTGAACAGCC TCATCGGGCA GCAGCAGCTC 
CAGCCGCTGT CCCACCACGC ACCCCCTGTG CCCCTCACCC CCCGCCCAGC CGGGCTGGTG 
GGCGGCAGTG CTACGGGGCT GCTTGCTCTG TCTGGAGCCC TGGCTGCCCA GGCTCAGCTG 
GCGGCGGCTG TCAAGGAGGA CCGTGCGGGC GTGGAGGCCG AGGGGTCCAG AGTGGAGAGA 
GCCCCGAGCA GGAGTGCATC TCCCTCGCCC CCTGAGAGTC TCGTGGAGGA GGAGCGACCG 
AGTGGCCCTG GTGGTGGCGG GAAGCAGAGA GCAGATGAGA AGGAGCCATC AGGACCTTAT 
GAAAGCGACG AAGACAAGAG TGATTACAAT CTGGTGGTGG ACGAGGACCA ACCCTCAGAG 
CCCCCCAGCC CGGCTACCAC CCCCTGCGGA AAGGTACCCA TCTGCATTCC TGCCCGTCGG 
GACCTGGTGG ACAGTCCAGC CTCCTTGGCC TCTAGCCTTG GCTCACCGCT GCCTAGAGCC 
AAGGAGCTCA TCCTGAATGA CCTTCCCGCC AGCACTCCTG CCTCCAAATC CTGTGACTCC 
TCCCCGCCCC AGGACGCTTC CACCCCCGGG CCCAGCTCGG CCAGTCACCT CTGCCAGCTT 
GCTGCCAAGC CAGCACCTTC CACGGACAGC GTCGCCCTGA GGAGCCCCCT GACTCTGTCC 
AGTCCCTTCA CCACGTCCTT CAGCCTGGGC TCCCACAGCA CTCTCAACGG AGACCTCTCC 
GTGCCCAGCT CCTACGTCAG CCTCCACCTG TCCCCCCAGG TCAGCAGCTC TGTGGTGTAC 
GGACGCTCCC CCGTGATGGC ATTTGAGTCT CATCCCCATC TCCGAGGGTC ATCCGTCTCT 
TCCTCCCTAC CCAGCATCCC TGGGGGAAAG CCGGCCTACT CCTTCCACGT GTCTGCGGAC 
GGGCAGATGC AGCCGGTTCC CTTCCCCTCG GATGCACTGG TAGGCGCGGG CATCCCGCGG 
CACGCCCGGC AGCTGCACAC GCTGGCCCAT GGCGAGGTGG TCTGCGCGGT CACCATCAGC 
GGCTCCACAC AGCATGTGTA CACGGGCGGC AAGGGCTGTG TGAAGGTGTG GGACGTGGGC 
CAGCCTGGGG CCAAGACGCC CGTGGCCCAG CTCGACTGCC TGAACCGAGA CAACTACATT 
CGTTCCTGCA AGTTGCTGCC GGATGGCCGG AGTCTGATCG TGGGCGGTGA GGCCAGCACC 
TTGTCCATTT GGGACCTGGC GGCGCCCACC CCCCGTATCA AGGCCGAGCT GACTTCCTCA 
GCCCCAGCCT GCTACGCCCT GGCCGTCAGC CCCGACGCCA AGGTTTGCTT CTCCTGCTGC 
AGCGATGGCA ACATTGTGGT CTGGGACCTG CAGAATCAGA CTATGGTCAG GCAGTTCCAG 
GGCCACACGG ACGGCGCCAG CTGCATTGAT ATTTCCGATT ACGGCACTCG GCTCTGGACA 
GGGGGCCTGG ACAACACGGT GCGCTGCTGG GACCTGCGGG AGGGCCGCCA GCTGCAGCAG 
CATGACTTCA GCTCCCAGAT TTTCTCCCTG GGCCACTGCC CTAACCAGGA CTGGCTGGCG 
GTCGGAATGG AGAGTAGCAA CGTGGAGATC CTGCACGTCC GCAAGCCGGA GAAATACCAG 
CTGCACCTCC ACGAGAGCTG CGTGCTGTCC CTGAAGTTTG CCTCCTGCGT CCAAGGAGTC 
GTCCTCAGTC CTGAGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLE2 ( XM_006722864.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722927.1 Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant X2, mRNA.

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CloneID	OHu51785
Clone ID Related Accession (Same CDS sequence)	XM_006722864.2
Accession Version	XM_006722864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006722864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGACGCTCC CCCGTGATGG CATTTGAGTC TCATCCCCAT 
CTCCGAGGGT CATCCGTCTC TTCCTCCCTA CCCAGCATCC CTGGGGGAAA GCCGGCCTAC 
TCCTTCCACG TGTCTGCGGA CGGGCAGATG CAGCCGGTTC CCTTCCCCTC GGATGCACTG 
GTAGGCGCGG GCATCCCGCG GCACGCCCGG CAGCTGCACA CGCTGGCCCA TGGCGAGGTG 
GTCTGCGCGG TCACCATCAG CGGCTCCACA CAGCATGTGT ACACGGGCGG CAAGGGCTGT 
GTGAAGGTGT GGGACGTGGG CCAGCCTGGG GCCAAGACGC CCGTGGCCCA GCTCGACTGC 
CTGAACCGAG ACAACTACAT TCGTTCCTGC AAGTTGCTGC CGGATGGCCG GAGTCTGATC 
GTGGGCGGTG AGGCCAGCAC CTTGTCCATT TGGGACCTGG CGGCGCCCAC CCCCCGTATC 
AAGGCCGAGC TGACTTCCTC AGCCCCAGCC TGCTACGCCC TGGCCGTCAG CCCCGACGCC 
AAGGTTTGCT TCTCCTGCTG CAGCGATGGC AACATTGTGG TCTGGGACCT GCAGAATCAG 
ACTATGGTCA GGCAGTTCCA GGGCCACACG GACGGCGCCA GCTGCATTGA TATTTCCGAT 
TACGGCACTC GGCTCTGGAC AGGGGGCCTG GACAACACGG TGCGCTGCTG GGACCTGCGG 
GAGGGCCGCC AGCTGCAGCA GCATGACTTC AGCTCCCAGA TTTTCTCCCT GGGCCACTGC 
CCTAACCAGG ACTGGCTGGC GGTCGGAATG GAGAGTAGCA ACGTGGAGAT CCTGCACGTC 
CGCAAGCCGG AGAAATACCA GCTGCACCTC CACGAGAGCT GCGTGCTGTC CCTGAAGTTT 
GCCTCCTGCG TCCAAGGAGT CGTCCTCAGT CCTGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722927.1
CDS	206..2287
Translation	MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCVQGVVLSPEL

Target ORF information:

RefSeq Version	XM_006722864.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722864.2

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGACGCTCC CCCGTGATGG CATTTGAGTC TCATCCCCAT 
CTCCGAGGGT CATCCGTCTC TTCCTCCCTA CCCAGCATCC CTGGGGGAAA GCCGGCCTAC 
TCCTTCCACG TGTCTGCGGA CGGGCAGATG CAGCCGGTTC CCTTCCCCTC GGATGCACTG 
GTAGGCGCGG GCATCCCGCG GCACGCCCGG CAGCTGCACA CGCTGGCCCA TGGCGAGGTG 
GTCTGCGCGG TCACCATCAG CGGCTCCACA CAGCATGTGT ACACGGGCGG CAAGGGCTGT 
GTGAAGGTGT GGGACGTGGG CCAGCCTGGG GCCAAGACGC CCGTGGCCCA GCTCGACTGC 
CTGAACCGAG ACAACTACAT TCGTTCCTGC AAGTTGCTGC CGGATGGCCG GAGTCTGATC 
GTGGGCGGTG AGGCCAGCAC CTTGTCCATT TGGGACCTGG CGGCGCCCAC CCCCCGTATC 
AAGGCCGAGC TGACTTCCTC AGCCCCAGCC TGCTACGCCC TGGCCGTCAG CCCCGACGCC 
AAGGTTTGCT TCTCCTGCTG CAGCGATGGC AACATTGTGG TCTGGGACCT GCAGAATCAG 
ACTATGGTCA GGCAGTTCCA GGGCCACACG GACGGCGCCA GCTGCATTGA TATTTCCGAT 
TACGGCACTC GGCTCTGGAC AGGGGGCCTG GACAACACGG TGCGCTGCTG GGACCTGCGG 
GAGGGCCGCC AGCTGCAGCA GCATGACTTC AGCTCCCAGA TTTTCTCCCT GGGCCACTGC 
CCTAACCAGG ACTGGCTGGC GGTCGGAATG GAGAGTAGCA ACGTGGAGAT CCTGCACGTC 
CGCAAGCCGG AGAAATACCA GCTGCACCTC CACGAGAGCT GCGTGCTGTC CCTGAAGTTT 
GCCTCCTGCG TCCAAGGAGT CGTCCTCAGT CCTGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLE2 ( XM_011528230.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526532.1 Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant X1, mRNA.

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CloneID	OHu61008
Clone ID Related Accession (Same CDS sequence)	XM_011528230.1
Accession Version	XM_011528230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
ATGGCATTTG AGTCTCATCC CCATCTCCGA GGGTCATCCG TCTCTTCCTC CCTACCCAGC 
ATCCCTGGGG GAAAGCCGGC CTACTCCTTC CACGTGTCTG CGGACGGGCA GATGCAGCCG 
GTTCCCTTCC CCTCGGATGC ACTGGTAGGC GCGGGCATCC CGCGGCACGC CCGGCAGCTG 
CACACGCTGG CCCATGGCGA GGTGGTCTGC GCGGTCACCA TCAGCGGCTC CACACAGCAT 
GTGTACACGG GCGGCAAGGG CTGTGTGAAG GTGTGGGACG TGGGCCAGCC TGGGGCCAAG 
ACGCCCGTGG CCCAGCTCGA CTGCCTGAAC CGAGACAACT ACATTCGTTC CTGCAAGTTG 
CTGCCGGATG GCCGGAGTCT GATCGTGGGC GGTGAGGCCA GCACCTTGTC CATTTGGGAC 
CTGGCGGCGC CCACCCCCCG TATCAAGGCC GAGCTGACTT CCTCAGCCCC AGCCTGCTAC 
GCCCTGGCCG TCAGCCCCGA CGCCAAGGTT TGCTTCTCCT GCTGCAGCGA TGGCAACATT 
GTGGTCTGGG ACCTGCAGAA TCAGACTATG GTCAGGCAGT TCCAGGGCCA CACGGACGGC 
GCCAGCTGCA TTGATATTTC CGATTACGGC ACTCGGCTCT GGACAGGGGG CCTGGACAAC 
ACGGTGCGCT GCTGGGACCT GCGGGAGGGC CGCCAGCTGC AGCAGCATGA CTTCAGCTCC 
CAGATTTTCT CCCTGGGCCA CTGCCCTAAC CAGGACTGGC TGGCGGTCGG AATGGAGAGT 
AGCAACGTGG AGATCCTGCA CGTCCGCAAG CCGGAGAAAT ACCAGCTGCA CCTCCACGAG 
AGCTGCGTGC TGTCCCTGAA GTTTGCCTCC TGCGGACGGT GGTTTGTGAG CACCGGGAAG 
GACAACCTGC TCAACGCCTG GAGGACGCCG TACGGGGCCA GCATTTTCCA GTCCAAGGAG 
TCGTCCTCAG TCCTGAGTTG TGACATCTCC AGAAATAACA AATACATCGT GACAGGCTCG 
GGGGACAAGA AGGCCACCGT GTATGAGGTG GTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526532.1
CDS	206..2404
Translation	MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_011528230.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528230.1

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
ATGGCATTTG AGTCTCATCC CCATCTCCGA GGGTCATCCG TCTCTTCCTC CCTACCCAGC 
ATCCCTGGGG GAAAGCCGGC CTACTCCTTC CACGTGTCTG CGGACGGGCA GATGCAGCCG 
GTTCCCTTCC CCTCGGATGC ACTGGTAGGC GCGGGCATCC CGCGGCACGC CCGGCAGCTG 
CACACGCTGG CCCATGGCGA GGTGGTCTGC GCGGTCACCA TCAGCGGCTC CACACAGCAT 
GTGTACACGG GCGGCAAGGG CTGTGTGAAG GTGTGGGACG TGGGCCAGCC TGGGGCCAAG 
ACGCCCGTGG CCCAGCTCGA CTGCCTGAAC CGAGACAACT ACATTCGTTC CTGCAAGTTG 
CTGCCGGATG GCCGGAGTCT GATCGTGGGC GGTGAGGCCA GCACCTTGTC CATTTGGGAC 
CTGGCGGCGC CCACCCCCCG TATCAAGGCC GAGCTGACTT CCTCAGCCCC AGCCTGCTAC 
GCCCTGGCCG TCAGCCCCGA CGCCAAGGTT TGCTTCTCCT GCTGCAGCGA TGGCAACATT 
GTGGTCTGGG ACCTGCAGAA TCAGACTATG GTCAGGCAGT TCCAGGGCCA CACGGACGGC 
GCCAGCTGCA TTGATATTTC CGATTACGGC ACTCGGCTCT GGACAGGGGG CCTGGACAAC 
ACGGTGCGCT GCTGGGACCT GCGGGAGGGC CGCCAGCTGC AGCAGCATGA CTTCAGCTCC 
CAGATTTTCT CCCTGGGCCA CTGCCCTAAC CAGGACTGGC TGGCGGTCGG AATGGAGAGT 
AGCAACGTGG AGATCCTGCA CGTCCGCAAG CCGGAGAAAT ACCAGCTGCA CCTCCACGAG 
AGCTGCGTGC TGTCCCTGAA GTTTGCCTCC TGCGGACGGT GGTTTGTGAG CACCGGGAAG 
GACAACCTGC TCAACGCCTG GAGGACGCCG TACGGGGCCA GCATTTTCCA GTCCAAGGAG 
TCGTCCTCAG TCCTGAGTTG TGACATCTCC AGAAATAACA AATACATCGT GACAGGCTCG 
GGGGACAAGA AGGCCACCGT GTATGAGGTG GTCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLE2 ( NM_003260.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003251.2 Homo sapiens transducin like enhancer of split 2 (TLE2), transcript variant 1, mRNA.

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CloneID	OHu09535
Clone ID Related Accession (Same CDS sequence)	NM_003260.4 , NM_003260.5
Accession Version	NM_003260.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW262763.1 and BC017364.2. On Jan 23, 2009 this sequence version replaced NM_003260.3. Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 1, which is shorter by an amino acid, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017364.2, SRR1660807.186768.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCTCCA GCCGCTGTCC CACCACGCAC CCCCTGTGCC CCTCACCCCC 
CGCCCAGCCG GGCTGGTGGG CGGCAGTGCT ACGGGGCTGC TTGCTCTGTC TGGAGCCCTG 
GCTGCCCAGG CTCAGCTGGC GGCGGCTGTC AAGGAGGACC GTGCGGGCGT GGAGGCCGAG 
GGGTCCAGAG TGGAGAGAGC CCCGAGCAGG AGTGCATCTC CCTCGCCCCC TGAGAGTCTC 
GTGGAGGAGG AGCGACCGAG TGGCCCTGGT GGTGGCGGGA AGCAGAGAGC AGATGAGAAG 
GAGCCATCAG GACCTTATGA AAGCGACGAA GACAAGAGTG ATTACAATCT GGTGGTGGAC 
GAGGACCAAC CCTCAGAGCC CCCCAGCCCG GCTACCACCC CCTGCGGAAA GGTACCCATC 
TGCATTCCTG CCCGTCGGGA CCTGGTGGAC AGTCCAGCCT CCTTGGCCTC TAGCCTTGGC 
TCACCGCTGC CTAGAGCCAA GGAGCTCATC CTGAATGACC TTCCCGCCAG CACTCCTGCC 
TCCAAATCCT GTGACTCCTC CCCGCCCCAG GACGCTTCCA CCCCCGGGCC CAGCTCGGCC 
AGTCACCTCT GCCAGCTTGC TGCCAAGCCA GCACCTTCCA CGGACAGCGT CGCCCTGAGG 
AGCCCCCTGA CTCTGTCCAG TCCCTTCACC ACGTCCTTCA GCCTGGGCTC CCACAGCACT 
CTCAACGGAG ACCTCTCCGT GCCCAGCTCC TACGTCAGCC TCCACCTGTC CCCCCAGGTC 
AGCAGCTCTG TGGTGTACGG ACGCTCCCCC GTGATGGCAT TTGAGTCTCA TCCCCATCTC 
CGAGGGTCAT CCGTCTCTTC CTCCCTACCC AGCATCCCTG GGGGAAAGCC GGCCTACTCC 
TTCCACGTGT CTGCGGACGG GCAGATGCAG CCGGTTCCCT TCCCCTCGGA TGCACTGGTA 
GGCGCGGGCA TCCCGCGGCA CGCCCGGCAG CTGCACACGC TGGCCCATGG CGAGGTGGTC 
TGCGCGGTCA CCATCAGCGG CTCCACACAG CATGTGTACA CGGGCGGCAA GGGCTGTGTG 
AAGGTGTGGG ACGTGGGCCA GCCTGGGGCC AAGACGCCCG TGGCCCAGCT CGACTGCCTG 
AACCGAGACA ACTACATTCG TTCCTGCAAG TTGCTGCCGG ATGGCCGGAG TCTGATCGTG 
GGCGGTGAGG CCAGCACCTT GTCCATTTGG GACCTGGCGG CGCCCACCCC CCGTATCAAG 
GCCGAGCTGA CTTCCTCAGC CCCAGCCTGC TACGCCCTGG CCGTCAGCCC CGACGCCAAG 
GTTTGCTTCT CCTGCTGCAG CGATGGCAAC ATTGTGGTCT GGGACCTGCA GAATCAGACT 
ATGGTCAGGC AGTTCCAGGG CCACACGGAC GGCGCCAGCT GCATTGATAT TTCCGATTAC 
GGCACTCGGC TCTGGACAGG GGGCCTGGAC AACACGGTGC GCTGCTGGGA CCTGCGGGAG 
GGCCGCCAGC TGCAGCAGCA TGACTTCAGC TCCCAGATTT TCTCCCTGGG CCACTGCCCT 
AACCAGGACT GGCTGGCGGT CGGAATGGAG AGTAGCAACG TGGAGATCCT GCACGTCCGC 
AAGCCGGAGA AATACCAGCT GCACCTCCAC GAGAGCTGCG TGCTGTCCCT GAAGTTTGCC 
TCCTGCGGAC GGTGGTTTGT GAGCACCGGG AAGGACAACC TGCTCAACGC CTGGAGGACG 
CCGTACGGGG CCAGCATTTT CCAGTCCAAG GAGTCGTCCT CAGTCCTGAG TTGTGACATC 
TCCAGAAATA ACAAATACAT CGTGACAGGC TCGGGGGACA AGAAGGCCAC CGTGTATGAG 
GTGGTCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003251.2
CDS	264..2495
Translation	MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	NM_003260.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transducin like enhancer of split 2 (TLE2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003260.4

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCTCCA GCCGCTGTCC CACCACGCAC CCCCTGTGCC CCTCACCCCC 
CGCCCAGCCG GGCTGGTGGG CGGCAGTGCT ACGGGGCTGC TTGCTCTGTC TGGAGCCCTG 
GCTGCCCAGG CTCAGCTGGC GGCGGCTGTC AAGGAGGACC GTGCGGGCGT GGAGGCCGAG 
GGGTCCAGAG TGGAGAGAGC CCCGAGCAGG AGTGCATCTC CCTCGCCCCC TGAGAGTCTC 
GTGGAGGAGG AGCGACCGAG TGGCCCTGGT GGTGGCGGGA AGCAGAGAGC AGATGAGAAG 
GAGCCATCAG GACCTTATGA AAGCGACGAA GACAAGAGTG ATTACAATCT GGTGGTGGAC 
GAGGACCAAC CCTCAGAGCC CCCCAGCCCG GCTACCACCC CCTGCGGAAA GGTACCCATC 
TGCATTCCTG CCCGTCGGGA CCTGGTGGAC AGTCCAGCCT CCTTGGCCTC TAGCCTTGGC 
TCACCGCTGC CTAGAGCCAA GGAGCTCATC CTGAATGACC TTCCCGCCAG CACTCCTGCC 
TCCAAATCCT GTGACTCCTC CCCGCCCCAG GACGCTTCCA CCCCCGGGCC CAGCTCGGCC 
AGTCACCTCT GCCAGCTTGC TGCCAAGCCA GCACCTTCCA CGGACAGCGT CGCCCTGAGG 
AGCCCCCTGA CTCTGTCCAG TCCCTTCACC ACGTCCTTCA GCCTGGGCTC CCACAGCACT 
CTCAACGGAG ACCTCTCCGT GCCCAGCTCC TACGTCAGCC TCCACCTGTC CCCCCAGGTC 
AGCAGCTCTG TGGTGTACGG ACGCTCCCCC GTGATGGCAT TTGAGTCTCA TCCCCATCTC 
CGAGGGTCAT CCGTCTCTTC CTCCCTACCC AGCATCCCTG GGGGAAAGCC GGCCTACTCC 
TTCCACGTGT CTGCGGACGG GCAGATGCAG CCGGTTCCCT TCCCCTCGGA TGCACTGGTA 
GGCGCGGGCA TCCCGCGGCA CGCCCGGCAG CTGCACACGC TGGCCCATGG CGAGGTGGTC 
TGCGCGGTCA CCATCAGCGG CTCCACACAG CATGTGTACA CGGGCGGCAA GGGCTGTGTG 
AAGGTGTGGG ACGTGGGCCA GCCTGGGGCC AAGACGCCCG TGGCCCAGCT CGACTGCCTG 
AACCGAGACA ACTACATTCG TTCCTGCAAG TTGCTGCCGG ATGGCCGGAG TCTGATCGTG 
GGCGGTGAGG CCAGCACCTT GTCCATTTGG GACCTGGCGG CGCCCACCCC CCGTATCAAG 
GCCGAGCTGA CTTCCTCAGC CCCAGCCTGC TACGCCCTGG CCGTCAGCCC CGACGCCAAG 
GTTTGCTTCT CCTGCTGCAG CGATGGCAAC ATTGTGGTCT GGGACCTGCA GAATCAGACT 
ATGGTCAGGC AGTTCCAGGG CCACACGGAC GGCGCCAGCT GCATTGATAT TTCCGATTAC 
GGCACTCGGC TCTGGACAGG GGGCCTGGAC AACACGGTGC GCTGCTGGGA CCTGCGGGAG 
GGCCGCCAGC TGCAGCAGCA TGACTTCAGC TCCCAGATTT TCTCCCTGGG CCACTGCCCT 
AACCAGGACT GGCTGGCGGT CGGAATGGAG AGTAGCAACG TGGAGATCCT GCACGTCCGC 
AAGCCGGAGA AATACCAGCT GCACCTCCAC GAGAGCTGCG TGCTGTCCCT GAAGTTTGCC 
TCCTGCGGAC GGTGGTTTGT GAGCACCGGG AAGGACAACC TGCTCAACGC CTGGAGGACG 
CCGTACGGGG CCAGCATTTT CCAGTCCAAG GAGTCGTCCT CAGTCCTGAG TTGTGACATC 
TCCAGAAATA ACAAATACAT CGTGACAGGC TCGGGGGACA AGAAGGCCAC CGTGTATGAG 
GTGGTCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLE2 ( NM_003260.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003251.2 Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 1, mRNA.

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CloneID	OHu09535
Clone ID Related Accession (Same CDS sequence)	NM_003260.4 , NM_003260.5
Accession Version	NM_003260.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011549.7, AW262763.1 and BC017364.2. On Nov 22, 2018 this sequence version replaced NM_003260.4. Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 1, which is shorter by an amino acid, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017364.2, SRR1660807.186768.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262953.11/ ENSP00000262953.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCTCCA GCCGCTGTCC CACCACGCAC CCCCTGTGCC CCTCACCCCC 
CGCCCAGCCG GGCTGGTGGG CGGCAGTGCT ACGGGGCTGC TTGCTCTGTC TGGAGCCCTG 
GCTGCCCAGG CTCAGCTGGC GGCGGCTGTC AAGGAGGACC GTGCGGGCGT GGAGGCCGAG 
GGGTCCAGAG TGGAGAGAGC CCCGAGCAGG AGTGCATCTC CCTCGCCCCC TGAGAGTCTC 
GTGGAGGAGG AGCGACCGAG TGGCCCTGGT GGTGGCGGGA AGCAGAGAGC AGATGAGAAG 
GAGCCATCAG GACCTTATGA AAGCGACGAA GACAAGAGTG ATTACAATCT GGTGGTGGAC 
GAGGACCAAC CCTCAGAGCC CCCCAGCCCG GCTACCACCC CCTGCGGAAA GGTACCCATC 
TGCATTCCTG CCCGTCGGGA CCTGGTGGAC AGTCCAGCCT CCTTGGCCTC TAGCCTTGGC 
TCACCGCTGC CTAGAGCCAA GGAGCTCATC CTGAATGACC TTCCCGCCAG CACTCCTGCC 
TCCAAATCCT GTGACTCCTC CCCGCCCCAG GACGCTTCCA CCCCCGGGCC CAGCTCGGCC 
AGTCACCTCT GCCAGCTTGC TGCCAAGCCA GCACCTTCCA CGGACAGCGT CGCCCTGAGG 
AGCCCCCTGA CTCTGTCCAG TCCCTTCACC ACGTCCTTCA GCCTGGGCTC CCACAGCACT 
CTCAACGGAG ACCTCTCCGT GCCCAGCTCC TACGTCAGCC TCCACCTGTC CCCCCAGGTC 
AGCAGCTCTG TGGTGTACGG ACGCTCCCCC GTGATGGCAT TTGAGTCTCA TCCCCATCTC 
CGAGGGTCAT CCGTCTCTTC CTCCCTACCC AGCATCCCTG GGGGAAAGCC GGCCTACTCC 
TTCCACGTGT CTGCGGACGG GCAGATGCAG CCGGTTCCCT TCCCCTCGGA TGCACTGGTA 
GGCGCGGGCA TCCCGCGGCA CGCCCGGCAG CTGCACACGC TGGCCCATGG CGAGGTGGTC 
TGCGCGGTCA CCATCAGCGG CTCCACACAG CATGTGTACA CGGGCGGCAA GGGCTGTGTG 
AAGGTGTGGG ACGTGGGCCA GCCTGGGGCC AAGACGCCCG TGGCCCAGCT CGACTGCCTG 
AACCGAGACA ACTACATTCG TTCCTGCAAG TTGCTGCCGG ATGGCCGGAG TCTGATCGTG 
GGCGGTGAGG CCAGCACCTT GTCCATTTGG GACCTGGCGG CGCCCACCCC CCGTATCAAG 
GCCGAGCTGA CTTCCTCAGC CCCAGCCTGC TACGCCCTGG CCGTCAGCCC CGACGCCAAG 
GTTTGCTTCT CCTGCTGCAG CGATGGCAAC ATTGTGGTCT GGGACCTGCA GAATCAGACT 
ATGGTCAGGC AGTTCCAGGG CCACACGGAC GGCGCCAGCT GCATTGATAT TTCCGATTAC 
GGCACTCGGC TCTGGACAGG GGGCCTGGAC AACACGGTGC GCTGCTGGGA CCTGCGGGAG 
GGCCGCCAGC TGCAGCAGCA TGACTTCAGC TCCCAGATTT TCTCCCTGGG CCACTGCCCT 
AACCAGGACT GGCTGGCGGT CGGAATGGAG AGTAGCAACG TGGAGATCCT GCACGTCCGC 
AAGCCGGAGA AATACCAGCT GCACCTCCAC GAGAGCTGCG TGCTGTCCCT GAAGTTTGCC 
TCCTGCGGAC GGTGGTTTGT GAGCACCGGG AAGGACAACC TGCTCAACGC CTGGAGGACG 
CCGTACGGGG CCAGCATTTT CCAGTCCAAG GAGTCGTCCT CAGTCCTGAG TTGTGACATC 
TCCAGAAATA ACAAATACAT CGTGACAGGC TCGGGGGACA AGAAGGCCAC CGTGTATGAG 
GTGGTCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003251.2
CDS	377..2608
Translation	MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	NM_003260.5
Organism	Homo sapiens(human)
Definition	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003260.5

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCTCCA GCCGCTGTCC CACCACGCAC CCCCTGTGCC CCTCACCCCC 
CGCCCAGCCG GGCTGGTGGG CGGCAGTGCT ACGGGGCTGC TTGCTCTGTC TGGAGCCCTG 
GCTGCCCAGG CTCAGCTGGC GGCGGCTGTC AAGGAGGACC GTGCGGGCGT GGAGGCCGAG 
GGGTCCAGAG TGGAGAGAGC CCCGAGCAGG AGTGCATCTC CCTCGCCCCC TGAGAGTCTC 
GTGGAGGAGG AGCGACCGAG TGGCCCTGGT GGTGGCGGGA AGCAGAGAGC AGATGAGAAG 
GAGCCATCAG GACCTTATGA AAGCGACGAA GACAAGAGTG ATTACAATCT GGTGGTGGAC 
GAGGACCAAC CCTCAGAGCC CCCCAGCCCG GCTACCACCC CCTGCGGAAA GGTACCCATC 
TGCATTCCTG CCCGTCGGGA CCTGGTGGAC AGTCCAGCCT CCTTGGCCTC TAGCCTTGGC 
TCACCGCTGC CTAGAGCCAA GGAGCTCATC CTGAATGACC TTCCCGCCAG CACTCCTGCC 
TCCAAATCCT GTGACTCCTC CCCGCCCCAG GACGCTTCCA CCCCCGGGCC CAGCTCGGCC 
AGTCACCTCT GCCAGCTTGC TGCCAAGCCA GCACCTTCCA CGGACAGCGT CGCCCTGAGG 
AGCCCCCTGA CTCTGTCCAG TCCCTTCACC ACGTCCTTCA GCCTGGGCTC CCACAGCACT 
CTCAACGGAG ACCTCTCCGT GCCCAGCTCC TACGTCAGCC TCCACCTGTC CCCCCAGGTC 
AGCAGCTCTG TGGTGTACGG ACGCTCCCCC GTGATGGCAT TTGAGTCTCA TCCCCATCTC 
CGAGGGTCAT CCGTCTCTTC CTCCCTACCC AGCATCCCTG GGGGAAAGCC GGCCTACTCC 
TTCCACGTGT CTGCGGACGG GCAGATGCAG CCGGTTCCCT TCCCCTCGGA TGCACTGGTA 
GGCGCGGGCA TCCCGCGGCA CGCCCGGCAG CTGCACACGC TGGCCCATGG CGAGGTGGTC 
TGCGCGGTCA CCATCAGCGG CTCCACACAG CATGTGTACA CGGGCGGCAA GGGCTGTGTG 
AAGGTGTGGG ACGTGGGCCA GCCTGGGGCC AAGACGCCCG TGGCCCAGCT CGACTGCCTG 
AACCGAGACA ACTACATTCG TTCCTGCAAG TTGCTGCCGG ATGGCCGGAG TCTGATCGTG 
GGCGGTGAGG CCAGCACCTT GTCCATTTGG GACCTGGCGG CGCCCACCCC CCGTATCAAG 
GCCGAGCTGA CTTCCTCAGC CCCAGCCTGC TACGCCCTGG CCGTCAGCCC CGACGCCAAG 
GTTTGCTTCT CCTGCTGCAG CGATGGCAAC ATTGTGGTCT GGGACCTGCA GAATCAGACT 
ATGGTCAGGC AGTTCCAGGG CCACACGGAC GGCGCCAGCT GCATTGATAT TTCCGATTAC 
GGCACTCGGC TCTGGACAGG GGGCCTGGAC AACACGGTGC GCTGCTGGGA CCTGCGGGAG 
GGCCGCCAGC TGCAGCAGCA TGACTTCAGC TCCCAGATTT TCTCCCTGGG CCACTGCCCT 
AACCAGGACT GGCTGGCGGT CGGAATGGAG AGTAGCAACG TGGAGATCCT GCACGTCCGC 
AAGCCGGAGA AATACCAGCT GCACCTCCAC GAGAGCTGCG TGCTGTCCCT GAAGTTTGCC 
TCCTGCGGAC GGTGGTTTGT GAGCACCGGG AAGGACAACC TGCTCAACGC CTGGAGGACG 
CCGTACGGGG CCAGCATTTT CCAGTCCAAG GAGTCGTCCT CAGTCCTGAG TTGTGACATC 
TCCAGAAATA ACAAATACAT CGTGACAGGC TCGGGGGACA AGAAGGCCAC CGTGTATGAG 
GTGGTCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLE2 ( NM_001300846.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287775.1 Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 4, mRNA.

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CloneID	OHu51784
Clone ID Related Accession (Same CDS sequence)	NM_001300846.2 , NM_001300846.1
Accession Version	NM_001300846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011549.7, HY046309.1, HY140405.1, AK312285.1 and BC017364.2. On May 31, 2019 this sequence version replaced NM_001300846.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGACGCTCC CCCGTGATGG CATTTGAGTC TCATCCCCAT 
CTCCGAGGGT CATCCGTCTC TTCCTCCCTA CCCAGCATCC CTGGGGGAAA GCCGGCCTAC 
TCCTTCCACG TGTCTGCGGA CGGGCAGATG CAGCCGGTTC CCTTCCCCTC GGATGCACTG 
GTAGGCGCGG GCATCCCGCG GCACGCCCGG CAGCTGCACA CGCTGGCCCA TGGCGAGGTG 
GTCTGCGCGG TCACCATCAG CGGCTCCACA CAGCATGTGT ACACGGGCGG CAAGGGCTGT 
GTGAAGGTGT GGGACGTGGG CCAGCCTGGG GCCAAGACGC CCGTGGCCCA GCTCGACTGC 
CTGAACCGAG ACAACTACAT TCGTTCCTGC AAGTTGCTGC CGGATGGCCG GAGTCTGATC 
GTGGGCGGTG AGGCCAGCAC CTTGTCCATT TGGGACCTGG CGGCGCCCAC CCCCCGTATC 
AAGGCCGAGC TGACTTCCTC AGCCCCAGCC TGCTACGCCC TGGCCGTCAG CCCCGACGCC 
AAGGTTTGCT TCTCCTGCTG CAGCGATGGC AACATTGTGG TCTGGGACCT GCAGAATCAG 
ACTATGGTCA GGCAGTTCCA GGGCCACACG GACGGCGCCA GCTGCATTGA TATTTCCGAT 
TACGGCACTC GGCTCTGGAC AGGGGGCCTG GACAACACGG TGCGCTGCTG GGACCTGCGG 
GAGGGCCGCC AGCTGCAGCA GCATGACTTC AGCTCCCAGA TTTTCTCCCT GGGCCACTGC 
CCTAACCAGG ACTGGCTGGC GGTCGGAATG GAGAGTAGCA ACGTGGAGAT CCTGCACGTC 
CGCAAGCCGG AGAAATACCA GCTGCACCTC CACGAGAGCT GCGTGCTGTC CCTGAAGTTT 
GCCTCCTGCG GACGGTGGTT TGTGAGCACC GGGAAGGACA ACCTGCTCAA CGCCTGGAGG 
ACGCCGTACG GGGCCAGCAT TTTCCAGTCC AAGGAGTCGT CCTCAGTCCT GAGTTGTGAC 
ATCTCCAGAA ATAACAAATA CATCGTGACA GGCTCGGGGG ACAAGAAGGC CACCGTGTAT 
GAGGTGGTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287775.1
CDS	377..2611
Translation	MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	NM_001300846.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300846.2

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGACGCTCC CCCGTGATGG CATTTGAGTC TCATCCCCAT 
CTCCGAGGGT CATCCGTCTC TTCCTCCCTA CCCAGCATCC CTGGGGGAAA GCCGGCCTAC 
TCCTTCCACG TGTCTGCGGA CGGGCAGATG CAGCCGGTTC CCTTCCCCTC GGATGCACTG 
GTAGGCGCGG GCATCCCGCG GCACGCCCGG CAGCTGCACA CGCTGGCCCA TGGCGAGGTG 
GTCTGCGCGG TCACCATCAG CGGCTCCACA CAGCATGTGT ACACGGGCGG CAAGGGCTGT 
GTGAAGGTGT GGGACGTGGG CCAGCCTGGG GCCAAGACGC CCGTGGCCCA GCTCGACTGC 
CTGAACCGAG ACAACTACAT TCGTTCCTGC AAGTTGCTGC CGGATGGCCG GAGTCTGATC 
GTGGGCGGTG AGGCCAGCAC CTTGTCCATT TGGGACCTGG CGGCGCCCAC CCCCCGTATC 
AAGGCCGAGC TGACTTCCTC AGCCCCAGCC TGCTACGCCC TGGCCGTCAG CCCCGACGCC 
AAGGTTTGCT TCTCCTGCTG CAGCGATGGC AACATTGTGG TCTGGGACCT GCAGAATCAG 
ACTATGGTCA GGCAGTTCCA GGGCCACACG GACGGCGCCA GCTGCATTGA TATTTCCGAT 
TACGGCACTC GGCTCTGGAC AGGGGGCCTG GACAACACGG TGCGCTGCTG GGACCTGCGG 
GAGGGCCGCC AGCTGCAGCA GCATGACTTC AGCTCCCAGA TTTTCTCCCT GGGCCACTGC 
CCTAACCAGG ACTGGCTGGC GGTCGGAATG GAGAGTAGCA ACGTGGAGAT CCTGCACGTC 
CGCAAGCCGG AGAAATACCA GCTGCACCTC CACGAGAGCT GCGTGCTGTC CCTGAAGTTT 
GCCTCCTGCG GACGGTGGTT TGTGAGCACC GGGAAGGACA ACCTGCTCAA CGCCTGGAGG 
ACGCCGTACG GGGCCAGCAT TTTCCAGTCC AAGGAGTCGT CCTCAGTCCT GAGTTGTGAC 
ATCTCCAGAA ATAACAAATA CATCGTGACA GGCTCGGGGG ACAAGAAGGC CACCGTGTAT 
GAGGTGGTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLE2 ( NM_001300846.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287775.1 Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 4, mRNA.

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CloneID	OHu51784
Clone ID Related Accession (Same CDS sequence)	NM_001300846.2 , NM_001300846.1
Accession Version	NM_001300846.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	transducin-like enhancer protein 2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY046309.1, HY140405.1, AK312285.1 and BC017364.2. On Jul 15, 2014 this sequence version replaced XM_005259636.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGACGCTCC CCCGTGATGG CATTTGAGTC TCATCCCCAT 
CTCCGAGGGT CATCCGTCTC TTCCTCCCTA CCCAGCATCC CTGGGGGAAA GCCGGCCTAC 
TCCTTCCACG TGTCTGCGGA CGGGCAGATG CAGCCGGTTC CCTTCCCCTC GGATGCACTG 
GTAGGCGCGG GCATCCCGCG GCACGCCCGG CAGCTGCACA CGCTGGCCCA TGGCGAGGTG 
GTCTGCGCGG TCACCATCAG CGGCTCCACA CAGCATGTGT ACACGGGCGG CAAGGGCTGT 
GTGAAGGTGT GGGACGTGGG CCAGCCTGGG GCCAAGACGC CCGTGGCCCA GCTCGACTGC 
CTGAACCGAG ACAACTACAT TCGTTCCTGC AAGTTGCTGC CGGATGGCCG GAGTCTGATC 
GTGGGCGGTG AGGCCAGCAC CTTGTCCATT TGGGACCTGG CGGCGCCCAC CCCCCGTATC 
AAGGCCGAGC TGACTTCCTC AGCCCCAGCC TGCTACGCCC TGGCCGTCAG CCCCGACGCC 
AAGGTTTGCT TCTCCTGCTG CAGCGATGGC AACATTGTGG TCTGGGACCT GCAGAATCAG 
ACTATGGTCA GGCAGTTCCA GGGCCACACG GACGGCGCCA GCTGCATTGA TATTTCCGAT 
TACGGCACTC GGCTCTGGAC AGGGGGCCTG GACAACACGG TGCGCTGCTG GGACCTGCGG 
GAGGGCCGCC AGCTGCAGCA GCATGACTTC AGCTCCCAGA TTTTCTCCCT GGGCCACTGC 
CCTAACCAGG ACTGGCTGGC GGTCGGAATG GAGAGTAGCA ACGTGGAGAT CCTGCACGTC 
CGCAAGCCGG AGAAATACCA GCTGCACCTC CACGAGAGCT GCGTGCTGTC CCTGAAGTTT 
GCCTCCTGCG GACGGTGGTT TGTGAGCACC GGGAAGGACA ACCTGCTCAA CGCCTGGAGG 
ACGCCGTACG GGGCCAGCAT TTTCCAGTCC AAGGAGTCGT CCTCAGTCCT GAGTTGTGAC 
ATCTCCAGAA ATAACAAATA CATCGTGACA GGCTCGGGGG ACAAGAAGGC CACCGTGTAT 
GAGGTGGTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287775.1
CDS	298..2532
Translation	MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQLQPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	NM_001300846.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TLE family member 2, transcriptional corepressor (TLE2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300846.1

ORF Insert Sequence:

ATGTACCCCC AGGGAAGGCA CCCGACCCCG CTCCAGTCCG GCCAGCCCTT CAAGTTCTCG 
ATCTTGGAGA TCTGCGACCG CATCAAAGAA GAATTCCAGT TTCTTCAGGC TCAATACCAC 
AGCCTCAAGC TAGAATGTGA GAAGCTGGCC AGCGAGAAGA CGGAAATGCA GCGACATTAT 
GTCATGTATT ATGAGATGTC GTACGGGCTC AACATTGAAA TGCATAAGCA GGCGGAGATT 
GTGAAGCGTC TGAGCGGTAT CTGCGCTCAG ATTATCCCCT TCCTGACCCA GGAGCATCAG 
CAGCAGGTGC TCCAGGCCGT AGAACGCGCC AAGCAGGTCA CCGTGGGGGA GCTGAACAGC 
CTCATCGGGC AGCAGCAGCT CCAGCCGCTG TCCCACCACG CACCCCCTGT GCCCCTCACC 
CCCCGCCCAG CCGGGCTGGT GGGCGGCAGT GCTACGGGGC TGCTTGCTCT GTCTGGAGCC 
CTGGCTGCCC AGGCTCAGCT GGCGGCGGCT GTCAAGGAGG ACCGTGCGGG CGTGGAGGCC 
GAGGGGTCCA GAGTGGAGAG AGCCCCGAGC AGGAGTGCAT CTCCCTCGCC CCCTGAGAGT 
CTCGTGGAGG AGGAGCGACC GAGTGGCCCT GGTGGTGGCG GGAAGCAGAG AGCAGATGAG 
AAGGAGCCAT CAGGACCTTA TGAAAGCGAC GAAGACAAGA GTGATTACAA TCTGGTGGTG 
GACGAGGACC AACCCTCAGA GCCCCCCAGC CCGGCTACCA CCCCCTGCGG AAAGGTACCC 
ATCTGCATTC CTGCCCGTCG GGACCTGGTG GACAGTCCAG CCTCCTTGGC CTCTAGCCTT 
GGCTCACCGC TGCCTAGAGC CAAGGAGCTC ATCCTGAATG ACCTTCCCGC CAGCACTCCT 
GCCTCCAAAT CCTGTGACTC CTCCCCGCCC CAGGACGCTT CCACCCCCGG GCCCAGCTCG 
GCCAGTCACC TCTGCCAGCT TGCTGCCAAG CCAGCACCTT CCACGGACAG CGTCGCCCTG 
AGGAGCCCCC TGACTCTGTC CAGTCCCTTC ACCACGTCCT TCAGCCTGGG CTCCCACAGC 
ACTCTCAACG GAGACCTCTC CGTGCCCAGC TCCTACGTCA GCCTCCACCT GTCCCCCCAG 
GTCAGCAGCT CTGTGGTGTA CGGACGCTCC CCCGTGATGG CATTTGAGTC TCATCCCCAT 
CTCCGAGGGT CATCCGTCTC TTCCTCCCTA CCCAGCATCC CTGGGGGAAA GCCGGCCTAC 
TCCTTCCACG TGTCTGCGGA CGGGCAGATG CAGCCGGTTC CCTTCCCCTC GGATGCACTG 
GTAGGCGCGG GCATCCCGCG GCACGCCCGG CAGCTGCACA CGCTGGCCCA TGGCGAGGTG 
GTCTGCGCGG TCACCATCAG CGGCTCCACA CAGCATGTGT ACACGGGCGG CAAGGGCTGT 
GTGAAGGTGT GGGACGTGGG CCAGCCTGGG GCCAAGACGC CCGTGGCCCA GCTCGACTGC 
CTGAACCGAG ACAACTACAT TCGTTCCTGC AAGTTGCTGC CGGATGGCCG GAGTCTGATC 
GTGGGCGGTG AGGCCAGCAC CTTGTCCATT TGGGACCTGG CGGCGCCCAC CCCCCGTATC 
AAGGCCGAGC TGACTTCCTC AGCCCCAGCC TGCTACGCCC TGGCCGTCAG CCCCGACGCC 
AAGGTTTGCT TCTCCTGCTG CAGCGATGGC AACATTGTGG TCTGGGACCT GCAGAATCAG 
ACTATGGTCA GGCAGTTCCA GGGCCACACG GACGGCGCCA GCTGCATTGA TATTTCCGAT 
TACGGCACTC GGCTCTGGAC AGGGGGCCTG GACAACACGG TGCGCTGCTG GGACCTGCGG 
GAGGGCCGCC AGCTGCAGCA GCATGACTTC AGCTCCCAGA TTTTCTCCCT GGGCCACTGC 
CCTAACCAGG ACTGGCTGGC GGTCGGAATG GAGAGTAGCA ACGTGGAGAT CCTGCACGTC 
CGCAAGCCGG AGAAATACCA GCTGCACCTC CACGAGAGCT GCGTGCTGTC CCTGAAGTTT 
GCCTCCTGCG GACGGTGGTT TGTGAGCACC GGGAAGGACA ACCTGCTCAA CGCCTGGAGG 
ACGCCGTACG GGGCCAGCAT TTTCCAGTCC AAGGAGTCGT CCTCAGTCCT GAGTTGTGAC 
ATCTCCAGAA ATAACAAATA CATCGTGACA GGCTCGGGGG ACAAGAAGGC CACCGTGTAT 
GAGGTGGTCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
Böhm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,Kölbl H,Pfeiffer N,Grus FH

Cellular corepressor TLE2 inhibits replication-and-transcription- activator-mediated transactivation and lytic reactivation of Kaposi's sarcoma-associated herpesvirus.
Journal of virology84(4)2047-62(2010 Feb)
He Z,Liu Y,Liang D,Wang Z,Robertson ES,Lan K

Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression.
BMC cancer9159(2009 May)
Arce L,Pate KT,Waterman ML

Groucho/TLE/R-esp proteins associate with the nuclear matrix and repress RUNX (CBF(alpha)/AML/PEBP2(alpha)) dependent activation of tissue-specific gene transcription.
Journal of cell science113 ( Pt 12)2221-31(2000 Jun)
Javed A,Guo B,Hiebert S,Choi JY,Green J,Zhao SC,Osborne MA,Stifani S,Stein JL,Lian JB,van Wijnen AJ,Stein GS

Transducin-like Enhancer of split 2, a mammalian homologue of Drosophila Groucho, acts as a transcriptional repressor, interacts with Hairy/Enhancer of split proteins, and is expressed during neuronal development.
European journal of biochemistry258(2)339-49(1998 Dec)
Grbavec D,Lo R,Liu Y,Stifani S

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TLE2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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