BTK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BTK
Entrez Gene ID	695
Full Name	Bruton tyrosine kinase
Synonyms	AGMX1,AT,ATK,BPK,IMD1,PSCTK1,XLA
General protein information	Preferred Names Bruton tyrosine kinase Names tyrosine-protein kinase BTK B-cell progenitor kinase Bruton agammaglobulinemia tyrosine kinase Bruton's tyrosine kinase agammaglobulinaemia tyrosine kinase dominant-negative kinase-deficient Brutons tyrosine kinase truncated Bruton agammaglobulinemia tyrosine kinase tyrosine-protein kinase BTK isoform (lacking exon 13 to 17) tyrosine-protein kinase BTK isoform (lacking exon 14)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.1
Summary	The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013].
Disorder MIM:	300300
Disorder Html:	Agammaglobulinemia, X-linked 1, 300755 (3); Agammaglobulinemia and isolated hormone deficiency, 307200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287344.1	NP_001274273.1	tyrosine-protein kinase BTK isoform 3
NM_000061.2	NP_000052.1	tyrosine-protein kinase BTK isoform 1
NM_000061.3	NP_000052.1	tyrosine-protein kinase BTK isoform 1
NM_001287345.1	NP_001274274.1	tyrosine-protein kinase BTK isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Btk	NP_038510.2
Gallus gallus (chicken)	BTK	NP_989564.2
Rattus norvegicus (Norway rat)	Btk	NP_001007799.1
Homo sapiens (human)	BTK	NP_001274273.1
Macaca mulatta (Rhesus monkey)	BTK	XP_002806376.1
Bos taurus (cattle)	BTK	NP_001029761.1
Canis lupus familiaris (dog)	BTK	XP_549139.2
Danio rerio (zebrafish)	btk	XP_697087.4
Xenopus tropicalis (tropical clawed frog)	btk	NP_001123732.1
Pan troglodytes (chimpanzee)	BTK	XP_001139132.2

Gene Ontology
Bibliography

Related articles in PubMed

Clinical and genetic profiles of patients with X-linked agammaglobulinemia from southeast Turkey: Novel mutations in BTK gene.
Doğruel D, Serbes M, Şaşihüseyinoğlu AŞ, Yılmaz M, Altıntaş DU, Bişgin A
Allergologia et immunopathologia47(1)24-31(2019 Jan - Feb)

Damaging BTK Variant Demonstrated by Carrier, Allele-Specific BTK Expression in B Cells and Monocytes.
Bausch-Jurken MT, Hintermeyer M, Woodliff J, Chen S, Rymaszewski A, Verbsky J, Routes J
Journal of clinical immunology39(1)23-25(2019 01)

BTK: a two-faced effector in cancer and tumour suppression.
Rada M, Barlev N, Macip S
Cell death & disease9(11)1064(2018 10)

Targeting the C481S Ibrutinib-Resistance Mutation in Bruton's Tyrosine Kinase Using PROTAC-Mediated Degradation.
Buhimschi AD, Armstrong HA, Toure M, Jaime-Figueroa S, Chen TL, Lehman AM, Woyach JA, Johnson AJ, Byrd JC, Crews CM
Biochemistry57(26)3564-3575(2018 07)

The Role of Bruton's Tyrosine Kinase in Immune Cell Signaling and Systemic Autoimmunity.
Rip J, Van Der Ploeg EK, Hendriks RW, Corneth OBJ
Critical reviews in immunology38(1)17-62(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BTK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BTK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20257	NM_000061.3 Latest version!	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20412	NM_001287345.1 Latest version!	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu20921	NM_001287344.1 Latest version!	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu20257	NM_000061.2	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BTK ( NM_000061.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000052.1 Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 1, mRNA.

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CloneID	OHu20257
Clone ID Related Accession (Same CDS sequence)	NM_000061.2 , NM_000061.3
Accession Version	NM_000061.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558886400000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase BTK isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619542.1, BC109079.1, DB636737.1 and AK057105.1. This sequence is a reference standard in the RefSeqGene project. On Nov 18, 2008 this sequence version replaced NM_000061.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.474193.1, SRR1660803.237772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAG TGATTCTGGA GAGCATCTTT CTGAAGCGAT CCCAACAGAA AAAGAAAACA 
TCACCTCTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACCG TGCACAAACT CTCCTACTAT 
GAGTATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAGG GTTCAATAGA TGTTGAGAAG 
ATCACTTGTG TTGAAACAGT GGTTCCTGAA AAAAATCCTC CTCCAGAAAG ACAGATTCCG 
AGAAGAGGTG AAGAGTCCAG TGAAATGGAG CAAATTTCAA TCATTGAAAG GTTCCCTTAT 
CCCTTCCAGG TTGTATATGA TGAAGGGCCT CTCTACGTCT TCTCCCCAAC TGAAGAACTA 
AGGAAGCGGT GGATTCACCA GCTCAAAAAC GTAATCCGGT ACAACAGTGA TCTGGTTCAG 
AAATATCACC CTTGCTTCTG GATCGATGGG CAGTATCTCT GCTGCTCTCA GACAGCCAAA 
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT 
CACCGGAAGA CAAAAAAGCC TCTTCCCCCA ACGCCTGAGG AGGACCAGAT CTTGAAAAAG 
CCACTACCGC CTGAGCCAGC AGCAGCACCA GTCTCCACAA GTGAGCTGAA AAAGGTTGTG 
GCCCTTTATG ATTACATGCC AATGAATGCA AATGATCTAC AGCTGCGGAA GGGTGATGAA 
TATTTTATCT TGGAGGAAAG CAACTTACCA TGGTGGAGAG CACGAGATAA AAATGGGCAG 
GAAGGCTACA TTCCTAGTAA CTATGTCACT GAAGCAGAAG ACTCCATAGA AATGTATGAG 
TGGTATTCCA AACACATGAC TCGGAGTCAG GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA 
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGCA AATATACAGT GTCTGTGTTT 
GCTAAATCCA CAGGGGACCC TCAAGGGGTG ATACGTCATT ATGTTGTGTG TTCCACACCT 
CAGAGCCAGT ATTACCTGGC TGAGAAGCAC CTTTTCAGCA CCATCCCTGA GCTCATTAAC 
TACCATCAGC ACAACTCTGC AGGACTCATA TCCAGGCTCA AATATCCAGT GTCTCAACAA 
AACAAGAATG CACCTTCCAC TGCAGGCCTG GGATACGGAT CATGGGAAAT TGATCCAAAG 
GACCTGACCT TCTTGAAGGA GCTGGGGACT GGACAATTTG GGGTAGTGAA GTATGGGAAA 
TGGAGAGGCC AGTACGACGT GGCCATCAAG ATGATCAAAG AAGGCTCCAT GTCTGAAGAT 
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG 
TATGGCGTCT GCACCAAGCA GCGCCCCATC TTCATCATCA CTGAGTACAT GGCCAATGGC 
TGCCTCCTGA ACTACCTGAG GGAGATGCGC CACCGCTTCC AGACTCAGCA GCTGCTAGAG 
ATGTGCAAGG ATGTCTGTGA AGCCATGGAA TACCTGGAGT CAAAGCAGTT CCTTCACCGA 
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTTAAAGT ATCTGATTTC 
GGCCTGTCCA GGTATGTCCT GGATGATGAA TACACAAGCT CAGTAGGCTC CAAATTTCCA 
GTCCGGTGGT CCCCACCGGA AGTCCTGATG TATAGCAAGT TCAGCAGCAA ATCTGACATT 
TGGGCTTTTG GGGTTTTGAT GTGGGAAATT TACTCCCTGG GGAAGATGCC ATATGAGAGA 
TTTACTAACA GTGAGACTGC TGAACACATT GCCCAAGGCC TACGTCTCTA CAGGCCTCAT 
CTGGCTTCAG AGAAGGTATA TACCATCATG TACAGTTGCT GGCATGAGAA AGCAGATGAG 
CGTCCCACTT TCAAAATTCT TCTGAGCAAT ATTCTAGATG TCATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000052.1
CDS	194..2173
Translation	MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

Target ORF information:

RefSeq Version	NM_000061.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000061.2

ORF Insert Sequence:

ATGGCCGCAG TGATTCTGGA GAGCATCTTT CTGAAGCGAT CCCAACAGAA AAAGAAAACA 
TCACCTCTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACCG TGCACAAACT CTCCTACTAT 
GAGTATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAGG GTTCAATAGA TGTTGAGAAG 
ATCACTTGTG TTGAAACAGT GGTTCCTGAA AAAAATCCTC CTCCAGAAAG ACAGATTCCG 
AGAAGAGGTG AAGAGTCCAG TGAAATGGAG CAAATTTCAA TCATTGAAAG GTTCCCTTAT 
CCCTTCCAGG TTGTATATGA TGAAGGGCCT CTCTACGTCT TCTCCCCAAC TGAAGAACTA 
AGGAAGCGGT GGATTCACCA GCTCAAAAAC GTAATCCGGT ACAACAGTGA TCTGGTTCAG 
AAATATCACC CTTGCTTCTG GATCGATGGG CAGTATCTCT GCTGCTCTCA GACAGCCAAA 
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT 
CACCGGAAGA CAAAAAAGCC TCTTCCCCCA ACGCCTGAGG AGGACCAGAT CTTGAAAAAG 
CCACTACCGC CTGAGCCAGC AGCAGCACCA GTCTCCACAA GTGAGCTGAA AAAGGTTGTG 
GCCCTTTATG ATTACATGCC AATGAATGCA AATGATCTAC AGCTGCGGAA GGGTGATGAA 
TATTTTATCT TGGAGGAAAG CAACTTACCA TGGTGGAGAG CACGAGATAA AAATGGGCAG 
GAAGGCTACA TTCCTAGTAA CTATGTCACT GAAGCAGAAG ACTCCATAGA AATGTATGAG 
TGGTATTCCA AACACATGAC TCGGAGTCAG GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA 
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGCA AATATACAGT GTCTGTGTTT 
GCTAAATCCA CAGGGGACCC TCAAGGGGTG ATACGTCATT ATGTTGTGTG TTCCACACCT 
CAGAGCCAGT ATTACCTGGC TGAGAAGCAC CTTTTCAGCA CCATCCCTGA GCTCATTAAC 
TACCATCAGC ACAACTCTGC AGGACTCATA TCCAGGCTCA AATATCCAGT GTCTCAACAA 
AACAAGAATG CACCTTCCAC TGCAGGCCTG GGATACGGAT CATGGGAAAT TGATCCAAAG 
GACCTGACCT TCTTGAAGGA GCTGGGGACT GGACAATTTG GGGTAGTGAA GTATGGGAAA 
TGGAGAGGCC AGTACGACGT GGCCATCAAG ATGATCAAAG AAGGCTCCAT GTCTGAAGAT 
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG 
TATGGCGTCT GCACCAAGCA GCGCCCCATC TTCATCATCA CTGAGTACAT GGCCAATGGC 
TGCCTCCTGA ACTACCTGAG GGAGATGCGC CACCGCTTCC AGACTCAGCA GCTGCTAGAG 
ATGTGCAAGG ATGTCTGTGA AGCCATGGAA TACCTGGAGT CAAAGCAGTT CCTTCACCGA 
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTTAAAGT ATCTGATTTC 
GGCCTGTCCA GGTATGTCCT GGATGATGAA TACACAAGCT CAGTAGGCTC CAAATTTCCA 
GTCCGGTGGT CCCCACCGGA AGTCCTGATG TATAGCAAGT TCAGCAGCAA ATCTGACATT 
TGGGCTTTTG GGGTTTTGAT GTGGGAAATT TACTCCCTGG GGAAGATGCC ATATGAGAGA 
TTTACTAACA GTGAGACTGC TGAACACATT GCCCAAGGCC TACGTCTCTA CAGGCCTCAT 
CTGGCTTCAG AGAAGGTATA TACCATCATG TACAGTTGCT GGCATGAGAA AGCAGATGAG 
CGTCCCACTT TCAAAATTCT TCTGAGCAAT ATTCTAGATG TCATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTK ( NM_000061.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000052.1 Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20257
Clone ID Related Accession (Same CDS sequence)	NM_000061.2 , NM_000061.3
Accession Version	NM_000061.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576684800000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase BTK isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619542.1, BC109079.1, DB636737.1 and AK057105.1. On Dec 4, 2019 this sequence version replaced NM_000061.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314382.1, BC109079.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAG TGATTCTGGA GAGCATCTTT CTGAAGCGAT CCCAACAGAA AAAGAAAACA 
TCACCTCTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACCG TGCACAAACT CTCCTACTAT 
GAGTATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAGG GTTCAATAGA TGTTGAGAAG 
ATCACTTGTG TTGAAACAGT GGTTCCTGAA AAAAATCCTC CTCCAGAAAG ACAGATTCCG 
AGAAGAGGTG AAGAGTCCAG TGAAATGGAG CAAATTTCAA TCATTGAAAG GTTCCCTTAT 
CCCTTCCAGG TTGTATATGA TGAAGGGCCT CTCTACGTCT TCTCCCCAAC TGAAGAACTA 
AGGAAGCGGT GGATTCACCA GCTCAAAAAC GTAATCCGGT ACAACAGTGA TCTGGTTCAG 
AAATATCACC CTTGCTTCTG GATCGATGGG CAGTATCTCT GCTGCTCTCA GACAGCCAAA 
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT 
CACCGGAAGA CAAAAAAGCC TCTTCCCCCA ACGCCTGAGG AGGACCAGAT CTTGAAAAAG 
CCACTACCGC CTGAGCCAGC AGCAGCACCA GTCTCCACAA GTGAGCTGAA AAAGGTTGTG 
GCCCTTTATG ATTACATGCC AATGAATGCA AATGATCTAC AGCTGCGGAA GGGTGATGAA 
TATTTTATCT TGGAGGAAAG CAACTTACCA TGGTGGAGAG CACGAGATAA AAATGGGCAG 
GAAGGCTACA TTCCTAGTAA CTATGTCACT GAAGCAGAAG ACTCCATAGA AATGTATGAG 
TGGTATTCCA AACACATGAC TCGGAGTCAG GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA 
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGCA AATATACAGT GTCTGTGTTT 
GCTAAATCCA CAGGGGACCC TCAAGGGGTG ATACGTCATT ATGTTGTGTG TTCCACACCT 
CAGAGCCAGT ATTACCTGGC TGAGAAGCAC CTTTTCAGCA CCATCCCTGA GCTCATTAAC 
TACCATCAGC ACAACTCTGC AGGACTCATA TCCAGGCTCA AATATCCAGT GTCTCAACAA 
AACAAGAATG CACCTTCCAC TGCAGGCCTG GGATACGGAT CATGGGAAAT TGATCCAAAG 
GACCTGACCT TCTTGAAGGA GCTGGGGACT GGACAATTTG GGGTAGTGAA GTATGGGAAA 
TGGAGAGGCC AGTACGACGT GGCCATCAAG ATGATCAAAG AAGGCTCCAT GTCTGAAGAT 
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG 
TATGGCGTCT GCACCAAGCA GCGCCCCATC TTCATCATCA CTGAGTACAT GGCCAATGGC 
TGCCTCCTGA ACTACCTGAG GGAGATGCGC CACCGCTTCC AGACTCAGCA GCTGCTAGAG 
ATGTGCAAGG ATGTCTGTGA AGCCATGGAA TACCTGGAGT CAAAGCAGTT CCTTCACCGA 
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTTAAAGT ATCTGATTTC 
GGCCTGTCCA GGTATGTCCT GGATGATGAA TACACAAGCT CAGTAGGCTC CAAATTTCCA 
GTCCGGTGGT CCCCACCGGA AGTCCTGATG TATAGCAAGT TCAGCAGCAA ATCTGACATT 
TGGGCTTTTG GGGTTTTGAT GTGGGAAATT TACTCCCTGG GGAAGATGCC ATATGAGAGA 
TTTACTAACA GTGAGACTGC TGAACACATT GCCCAAGGCC TACGTCTCTA CAGGCCTCAT 
CTGGCTTCAG AGAAGGTATA TACCATCATG TACAGTTGCT GGCATGAGAA AGCAGATGAG 
CGTCCCACTT TCAAAATTCT TCTGAGCAAT ATTCTAGATG TCATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000052.1
CDS	161..2140
Translation	MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

Target ORF information:

RefSeq Version	NM_000061.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000061.3

ORF Insert Sequence:

ATGGCCGCAG TGATTCTGGA GAGCATCTTT CTGAAGCGAT CCCAACAGAA AAAGAAAACA 
TCACCTCTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACCG TGCACAAACT CTCCTACTAT 
GAGTATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAGG GTTCAATAGA TGTTGAGAAG 
ATCACTTGTG TTGAAACAGT GGTTCCTGAA AAAAATCCTC CTCCAGAAAG ACAGATTCCG 
AGAAGAGGTG AAGAGTCCAG TGAAATGGAG CAAATTTCAA TCATTGAAAG GTTCCCTTAT 
CCCTTCCAGG TTGTATATGA TGAAGGGCCT CTCTACGTCT TCTCCCCAAC TGAAGAACTA 
AGGAAGCGGT GGATTCACCA GCTCAAAAAC GTAATCCGGT ACAACAGTGA TCTGGTTCAG 
AAATATCACC CTTGCTTCTG GATCGATGGG CAGTATCTCT GCTGCTCTCA GACAGCCAAA 
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT 
CACCGGAAGA CAAAAAAGCC TCTTCCCCCA ACGCCTGAGG AGGACCAGAT CTTGAAAAAG 
CCACTACCGC CTGAGCCAGC AGCAGCACCA GTCTCCACAA GTGAGCTGAA AAAGGTTGTG 
GCCCTTTATG ATTACATGCC AATGAATGCA AATGATCTAC AGCTGCGGAA GGGTGATGAA 
TATTTTATCT TGGAGGAAAG CAACTTACCA TGGTGGAGAG CACGAGATAA AAATGGGCAG 
GAAGGCTACA TTCCTAGTAA CTATGTCACT GAAGCAGAAG ACTCCATAGA AATGTATGAG 
TGGTATTCCA AACACATGAC TCGGAGTCAG GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA 
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGCA AATATACAGT GTCTGTGTTT 
GCTAAATCCA CAGGGGACCC TCAAGGGGTG ATACGTCATT ATGTTGTGTG TTCCACACCT 
CAGAGCCAGT ATTACCTGGC TGAGAAGCAC CTTTTCAGCA CCATCCCTGA GCTCATTAAC 
TACCATCAGC ACAACTCTGC AGGACTCATA TCCAGGCTCA AATATCCAGT GTCTCAACAA 
AACAAGAATG CACCTTCCAC TGCAGGCCTG GGATACGGAT CATGGGAAAT TGATCCAAAG 
GACCTGACCT TCTTGAAGGA GCTGGGGACT GGACAATTTG GGGTAGTGAA GTATGGGAAA 
TGGAGAGGCC AGTACGACGT GGCCATCAAG ATGATCAAAG AAGGCTCCAT GTCTGAAGAT 
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG 
TATGGCGTCT GCACCAAGCA GCGCCCCATC TTCATCATCA CTGAGTACAT GGCCAATGGC 
TGCCTCCTGA ACTACCTGAG GGAGATGCGC CACCGCTTCC AGACTCAGCA GCTGCTAGAG 
ATGTGCAAGG ATGTCTGTGA AGCCATGGAA TACCTGGAGT CAAAGCAGTT CCTTCACCGA 
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTTAAAGT ATCTGATTTC 
GGCCTGTCCA GGTATGTCCT GGATGATGAA TACACAAGCT CAGTAGGCTC CAAATTTCCA 
GTCCGGTGGT CCCCACCGGA AGTCCTGATG TATAGCAAGT TCAGCAGCAA ATCTGACATT 
TGGGCTTTTG GGGTTTTGAT GTGGGAAATT TACTCCCTGG GGAAGATGCC ATATGAGAGA 
TTTACTAACA GTGAGACTGC TGAACACATT GCCCAAGGCC TACGTCTCTA CAGGCCTCAT 
CTGGCTTCAG AGAAGGTATA TACCATCATG TACAGTTGCT GGCATGAGAA AGCAGATGAG 
CGTCCCACTT TCAAAATTCT TCTGAGCAAT ATTCTAGATG TCATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTK ( NM_001287345.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274274.1 Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20412
Clone ID Related Accession (Same CDS sequence)	NM_001287345.1
Accession Version	NM_001287345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase BTK isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619542.1, AU107296.1, AK057105.1, AL035422.12 and BC109079.1. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and lacks several exons in the 3' CDS compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK057105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2149004, SAMEA2152568 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAG TGATTCTGGA GAGCATCTTT CTGAAGCGAT CCCAACAGAA AAAGAAAACA 
TCACCTCTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACCG TGCACAAACT CTCCTACTAT 
GAGTATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAGG GTTCAATAGA TGTTGAGAAG 
ATCACTTGTG TTGAAACAGT GGTTCCTGAA AAAAATCCTC CTCCAGAAAG ACAGATTCCG 
AGAAGAGGTG AAGAGTCCAG TGAAATGGAG CAAATTTCAA TCATTGAAAG GTTCCCTTAT 
CCCTTCCAGG TTGTATATGA TGAAGGGCCT CTCTACGTCT TCTCCCCAAC TGAAGAACTA 
AGGAAGCGGT GGATTCACCA GCTCAAAAAC GTAATCCGGT ACAACAGTGA TCTGGTTCAG 
AAATATCACC CTTGCTTCTG GATCGATGGG CAGTATCTCT GCTGCTCTCA GACAGCCAAA 
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT 
CACCGGAAGA CAAAAAAGCC TCTTCCCCCA ACGCCTGAGG AGGACCAGAT CTTGAAAAAG 
CCACTACCGC CTGAGCCAGC AGCAGCACCA GTCTCCACAA GTGAGCTGAA AAAGGTTGTG 
GCCCTTTATG ATTACATGCC AATGAATGCA AATGATCTAC AGCTGCGGAA GGGTGATGAA 
TATTTTATCT TGGAGGAAAG CAACTTACCA TGGTGGAGAG CACGAGATAA AAATGGGCAG 
GAAGGCTACA TTCCTAGTAA CTATGTCACT GAAGCAGAAG ACTCCATAGA AATGTATGAG 
TGGTATTCCA AACACATGAC TCGGAGTCAG GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA 
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGCA AATATACAGT GTCTGTGTTT 
GCTAAATCCA CAGGGGACCC TCAAGGGGTG ATACGTCATT ATGTTGTGTG TTCCACACCT 
CAGAGCCAGT ATTACCTGGC AGCTCGAAAC TGTTTGGTAA ACGATCAAGG AGTTGTTAAA 
GTATCTGATT TCGGCCTGTC CAGGTATGTC CTGGATGATG AATACACAAG CTCAGTAGGC 
TCCAAATTTC CAGTCCGGTG GTCCCCACCG GAAGTCCTGA TGTATAGCAA GTTCAGCAGC 
AAATCTGACA TTTGGGCTTT TGGGGTTTTG ATGTGGGAAA TTTACTCCCT GGGGAAGATG 
CCATATGAGA GATTTACTAA CAGTGAGACT GCTGAACACA TTGCCCAAGG CCTACGTCTC 
TACAGGCCTC ATCTGGCTTC AGAGAAGGTA TATACCATCA TGTACAGTTG CTGGCATGAG 
AAAGCAGATG AGCGTCCCAC TTTCAAAATT CTTCTGAGCA ATATTCTAGA TGTCATGGAT 
GAAGAATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274274.1
CDS	359..1810
Translation	MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

Target ORF information:

RefSeq Version	NM_001287345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287345.1

ORF Insert Sequence:

ATGGCCGCAG TGATTCTGGA GAGCATCTTT CTGAAGCGAT CCCAACAGAA AAAGAAAACA 
TCACCTCTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACCG TGCACAAACT CTCCTACTAT 
GAGTATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAGG GTTCAATAGA TGTTGAGAAG 
ATCACTTGTG TTGAAACAGT GGTTCCTGAA AAAAATCCTC CTCCAGAAAG ACAGATTCCG 
AGAAGAGGTG AAGAGTCCAG TGAAATGGAG CAAATTTCAA TCATTGAAAG GTTCCCTTAT 
CCCTTCCAGG TTGTATATGA TGAAGGGCCT CTCTACGTCT TCTCCCCAAC TGAAGAACTA 
AGGAAGCGGT GGATTCACCA GCTCAAAAAC GTAATCCGGT ACAACAGTGA TCTGGTTCAG 
AAATATCACC CTTGCTTCTG GATCGATGGG CAGTATCTCT GCTGCTCTCA GACAGCCAAA 
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT 
CACCGGAAGA CAAAAAAGCC TCTTCCCCCA ACGCCTGAGG AGGACCAGAT CTTGAAAAAG 
CCACTACCGC CTGAGCCAGC AGCAGCACCA GTCTCCACAA GTGAGCTGAA AAAGGTTGTG 
GCCCTTTATG ATTACATGCC AATGAATGCA AATGATCTAC AGCTGCGGAA GGGTGATGAA 
TATTTTATCT TGGAGGAAAG CAACTTACCA TGGTGGAGAG CACGAGATAA AAATGGGCAG 
GAAGGCTACA TTCCTAGTAA CTATGTCACT GAAGCAGAAG ACTCCATAGA AATGTATGAG 
TGGTATTCCA AACACATGAC TCGGAGTCAG GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA 
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGCA AATATACAGT GTCTGTGTTT 
GCTAAATCCA CAGGGGACCC TCAAGGGGTG ATACGTCATT ATGTTGTGTG TTCCACACCT 
CAGAGCCAGT ATTACCTGGC AGCTCGAAAC TGTTTGGTAA ACGATCAAGG AGTTGTTAAA 
GTATCTGATT TCGGCCTGTC CAGGTATGTC CTGGATGATG AATACACAAG CTCAGTAGGC 
TCCAAATTTC CAGTCCGGTG GTCCCCACCG GAAGTCCTGA TGTATAGCAA GTTCAGCAGC 
AAATCTGACA TTTGGGCTTT TGGGGTTTTG ATGTGGGAAA TTTACTCCCT GGGGAAGATG 
CCATATGAGA GATTTACTAA CAGTGAGACT GCTGAACACA TTGCCCAAGG CCTACGTCTC 
TACAGGCCTC ATCTGGCTTC AGAGAAGGTA TATACCATCA TGTACAGTTG CTGGCATGAG 
AAAGCAGATG AGCGTCCCAC TTTCAAAATT CTTCTGAGCA ATATTCTAGA TGTCATGGAT 
GAAGAATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTK ( NM_001287344.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274273.1 Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 3, mRNA.

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CloneID	OHu20921
Clone ID Related Accession (Same CDS sequence)	NM_001287344.1
Accession Version	NM_001287344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase BTK isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV733045.1, AK309851.1, DB636737.1 and DB331677.1. Transcript Variant: This variant (3) differs in the 5' UTR and CDS, and represents the use of an alternate promoter, compared to variant 1. The resulting protein (isoform 3) has a longer N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK309851.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGTT GGTCCATTCA ACAAATGGTT ATTGGATGCC CATTATGTGG CAGGCACTGT 
TCCGGGGGAG AGCACACAGG TGAACTCCAG AAAGAAGAAG CTATGGCCGC AGTGATTCTG 
GAGAGCATCT TTCTGAAGCG ATCCCAACAG AAAAAGAAAA CATCACCTCT AAACTTCAAG 
AAGCGCCTGT TTCTCTTGAC CGTGCACAAA CTCTCCTACT ATGAGTATGA CTTTGAACGT 
GGGAGAAGAG GCAGTAAGAA GGGTTCAATA GATGTTGAGA AGATCACTTG TGTTGAAACA 
GTGGTTCCTG AAAAAAATCC TCCTCCAGAA AGACAGATTC CGAGAAGAGG TGAAGAGTCC 
AGTGAAATGG AGCAAATTTC AATCATTGAA AGGTTCCCTT ATCCCTTCCA GGTTGTATAT 
GATGAAGGGC CTCTCTACGT CTTCTCCCCA ACTGAAGAAC TAAGGAAGCG GTGGATTCAC 
CAGCTCAAAA ACGTAATCCG GTACAACAGT GATCTGGTTC AGAAATATCA CCCTTGCTTC 
TGGATCGATG GGCAGTATCT CTGCTGCTCT CAGACAGCCA AAAATGCTAT GGGCTGCCAA 
ATTTTGGAGA ACAGGAATGG AAGCTTAAAA CCTGGGAGTT CTCACCGGAA GACAAAAAAG 
CCTCTTCCCC CAACGCCTGA GGAGGACCAG ATCTTGAAAA AGCCACTACC GCCTGAGCCA 
GCAGCAGCAC CAGTCTCCAC AAGTGAGCTG AAAAAGGTTG TGGCCCTTTA TGATTACATG 
CCAATGAATG CAAATGATCT ACAGCTGCGG AAGGGTGATG AATATTTTAT CTTGGAGGAA 
AGCAACTTAC CATGGTGGAG AGCACGAGAT AAAAATGGGC AGGAAGGCTA CATTCCTAGT 
AACTATGTCA CTGAAGCAGA AGACTCCATA GAAATGTATG AGTGGTATTC CAAACACATG 
ACTCGGAGTC AGGCTGAGCA ACTGCTAAAG CAAGAGGGGA AAGAAGGAGG TTTCATTGTC 
AGAGACTCCA GCAAAGCTGG CAAATATACA GTGTCTGTGT TTGCTAAATC CACAGGGGAC 
CCTCAAGGGG TGATACGTCA TTATGTTGTG TGTTCCACAC CTCAGAGCCA GTATTACCTG 
GCTGAGAAGC ACCTTTTCAG CACCATCCCT GAGCTCATTA ACTACCATCA GCACAACTCT 
GCAGGACTCA TATCCAGGCT CAAATATCCA GTGTCTCAAC AAAACAAGAA TGCACCTTCC 
ACTGCAGGCC TGGGATACGG ATCATGGGAA ATTGATCCAA AGGACCTGAC CTTCTTGAAG 
GAGCTGGGGA CTGGACAATT TGGGGTAGTG AAGTATGGGA AATGGAGAGG CCAGTACGAC 
GTGGCCATCA AGATGATCAA AGAAGGCTCC ATGTCTGAAG ATGAATTCAT TGAAGAAGCC 
AAAGTCATGA TGAATCTTTC CCATGAGAAG CTGGTGCAGT TGTATGGCGT CTGCACCAAG 
CAGCGCCCCA TCTTCATCAT CACTGAGTAC ATGGCCAATG GCTGCCTCCT GAACTACCTG 
AGGGAGATGC GCCACCGCTT CCAGACTCAG CAGCTGCTAG AGATGTGCAA GGATGTCTGT 
GAAGCCATGG AATACCTGGA GTCAAAGCAG TTCCTTCACC GAGACCTGGC AGCTCGAAAC 
TGTTTGGTAA ACGATCAAGG AGTTGTTAAA GTATCTGATT TCGGCCTGTC CAGGTATGTC 
CTGGATGATG AATACACAAG CTCAGTAGGC TCCAAATTTC CAGTCCGGTG GTCCCCACCG 
GAAGTCCTGA TGTATAGCAA GTTCAGCAGC AAATCTGACA TTTGGGCTTT TGGGGTTTTG 
ATGTGGGAAA TTTACTCCCT GGGGAAGATG CCATATGAGA GATTTACTAA CAGTGAGACT 
GCTGAACACA TTGCCCAAGG CCTACGTCTC TACAGGCCTC ATCTGGCTTC AGAGAAGGTA 
TATACCATCA TGTACAGTTG CTGGCATGAG AAAGCAGATG AGCGTCCCAC TTTCAAAATT 
CTTCTGAGCA ATATTCTAGA TGTCATGGAT GAAGAATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274273.1
CDS	248..2329
Translation	MASWSIQQMVIGCPLCGRHCSGGEHTGELQKEEAMAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

Target ORF information:

RefSeq Version	NM_001287344.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287344.1

ORF Insert Sequence:

ATGGCCAGTT GGTCCATTCA ACAAATGGTT ATTGGATGCC CATTATGTGG CAGGCACTGT 
TCCGGGGGAG AGCACACAGG TGAACTCCAG AAAGAAGAAG CTATGGCCGC AGTGATTCTG 
GAGAGCATCT TTCTGAAGCG ATCCCAACAG AAAAAGAAAA CATCACCTCT AAACTTCAAG 
AAGCGCCTGT TTCTCTTGAC CGTGCACAAA CTCTCCTACT ATGAGTATGA CTTTGAACGT 
GGGAGAAGAG GCAGTAAGAA GGGTTCAATA GATGTTGAGA AGATCACTTG TGTTGAAACA 
GTGGTTCCTG AAAAAAATCC TCCTCCAGAA AGACAGATTC CGAGAAGAGG TGAAGAGTCC 
AGTGAAATGG AGCAAATTTC AATCATTGAA AGGTTCCCTT ATCCCTTCCA GGTTGTATAT 
GATGAAGGGC CTCTCTACGT CTTCTCCCCA ACTGAAGAAC TAAGGAAGCG GTGGATTCAC 
CAGCTCAAAA ACGTAATCCG GTACAACAGT GATCTGGTTC AGAAATATCA CCCTTGCTTC 
TGGATCGATG GGCAGTATCT CTGCTGCTCT CAGACAGCCA AAAATGCTAT GGGCTGCCAA 
ATTTTGGAGA ACAGGAATGG AAGCTTAAAA CCTGGGAGTT CTCACCGGAA GACAAAAAAG 
CCTCTTCCCC CAACGCCTGA GGAGGACCAG ATCTTGAAAA AGCCACTACC GCCTGAGCCA 
GCAGCAGCAC CAGTCTCCAC AAGTGAGCTG AAAAAGGTTG TGGCCCTTTA TGATTACATG 
CCAATGAATG CAAATGATCT ACAGCTGCGG AAGGGTGATG AATATTTTAT CTTGGAGGAA 
AGCAACTTAC CATGGTGGAG AGCACGAGAT AAAAATGGGC AGGAAGGCTA CATTCCTAGT 
AACTATGTCA CTGAAGCAGA AGACTCCATA GAAATGTATG AGTGGTATTC CAAACACATG 
ACTCGGAGTC AGGCTGAGCA ACTGCTAAAG CAAGAGGGGA AAGAAGGAGG TTTCATTGTC 
AGAGACTCCA GCAAAGCTGG CAAATATACA GTGTCTGTGT TTGCTAAATC CACAGGGGAC 
CCTCAAGGGG TGATACGTCA TTATGTTGTG TGTTCCACAC CTCAGAGCCA GTATTACCTG 
GCTGAGAAGC ACCTTTTCAG CACCATCCCT GAGCTCATTA ACTACCATCA GCACAACTCT 
GCAGGACTCA TATCCAGGCT CAAATATCCA GTGTCTCAAC AAAACAAGAA TGCACCTTCC 
ACTGCAGGCC TGGGATACGG ATCATGGGAA ATTGATCCAA AGGACCTGAC CTTCTTGAAG 
GAGCTGGGGA CTGGACAATT TGGGGTAGTG AAGTATGGGA AATGGAGAGG CCAGTACGAC 
GTGGCCATCA AGATGATCAA AGAAGGCTCC ATGTCTGAAG ATGAATTCAT TGAAGAAGCC 
AAAGTCATGA TGAATCTTTC CCATGAGAAG CTGGTGCAGT TGTATGGCGT CTGCACCAAG 
CAGCGCCCCA TCTTCATCAT CACTGAGTAC ATGGCCAATG GCTGCCTCCT GAACTACCTG 
AGGGAGATGC GCCACCGCTT CCAGACTCAG CAGCTGCTAG AGATGTGCAA GGATGTCTGT 
GAAGCCATGG AATACCTGGA GTCAAAGCAG TTCCTTCACC GAGACCTGGC AGCTCGAAAC 
TGTTTGGTAA ACGATCAAGG AGTTGTTAAA GTATCTGATT TCGGCCTGTC CAGGTATGTC 
CTGGATGATG AATACACAAG CTCAGTAGGC TCCAAATTTC CAGTCCGGTG GTCCCCACCG 
GAAGTCCTGA TGTATAGCAA GTTCAGCAGC AAATCTGACA TTTGGGCTTT TGGGGTTTTG 
ATGTGGGAAA TTTACTCCCT GGGGAAGATG CCATATGAGA GATTTACTAA CAGTGAGACT 
GCTGAACACA TTGCCCAAGG CCTACGTCTC TACAGGCCTC ATCTGGCTTC AGAGAAGGTA 
TATACCATCA TGTACAGTTG CTGGCATGAG AAAGCAGATG AGCGTCCCAC TTTCAAAATT 
CTTCTGAGCA ATATTCTAGA TGTCATGGAT GAAGAATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical and genetic profiles of patients with X-linked agammaglobulinemia from southeast Turkey: Novel mutations in BTK gene.
Allergologia et immunopathologia47(1)24-31(2019 Jan - Feb)
Doğruel D,Serbes M,Şaşihüseyinoğlu AŞ,Yılmaz M,Altıntaş DU,Bişgin A

Damaging BTK Variant Demonstrated by Carrier, Allele-Specific BTK Expression in B Cells and Monocytes.
Journal of clinical immunology39(1)23-25(2019 01)
Bausch-Jurken MT,Hintermeyer M,Woodliff J,Chen S,Rymaszewski A,Verbsky J,Routes J

BTK: a two-faced effector in cancer and tumour suppression.
Cell death & disease9(11)1064(2018 10)
Rada M,Barlev N,Macip S

Targeting the C481S Ibrutinib-Resistance Mutation in Bruton's Tyrosine Kinase Using PROTAC-Mediated Degradation.
Biochemistry57(26)3564-3575(2018 07)
Buhimschi AD,Armstrong HA,Toure M,Jaime-Figueroa S,Chen TL,Lehman AM,Woyach JA,Johnson AJ,Byrd JC,Crews CM

The Role of Bruton's Tyrosine Kinase in Immune Cell Signaling and Systemic Autoimmunity.
Critical reviews in immunology38(1)17-62(2018)
Rip J,Van Der Ploeg EK,Hendriks RW,Corneth OBJ

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BTK cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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