NDST4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDST4
Entrez Gene ID	64579
Full Name	N-deacetylase and N-sulfotransferase 4
Synonyms	N-HSST,N-HSST 4,NDST-4,NHSST4
General protein information	Preferred Names N-deacetylase and N-sulfotransferase 4 Names bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 N-deacetylase/N-sulfotransferase 4 N-heparan sulfate sulfotransferase 4 glucosaminyl N-deacetylase/N-sulfotransferase 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q26
Disorder MIM:	615039

mRNA and Protein(s)

mRNA	Protein	Name
XM_017008545.2	XP_016864034.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 isoform X1
NM_022569.1	NP_072091.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
NM_022569.3	NP_072091.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
XM_017008546.1	XP_016864035.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 isoform X1
XM_017008545.1	XP_016864034.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 isoform X1
NM_022569.2	NP_072091.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NDST4	XP_001097335.1
Rattus norvegicus (Norway rat)	Ndst4	NP_001178778.1
Gallus gallus (chicken)	NDST4	XP_420638.2
Xenopus tropicalis (tropical clawed frog)	ndst4	XP_002934293.1
Homo sapiens (human)	NDST4	NP_072091.1
Mus musculus (house mouse)	Ndst4	NP_072087.2
Bos taurus (cattle)	NDST4	NP_001179602.1
Pan troglodytes (chimpanzee)	NDST4	XP_526668.1
Canis lupus familiaris (dog)	NDST4	XP_005639369.1
Danio rerio (zebrafish)	ndst3	XP_002660470.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of heparan sulfate N-deacetylase/N-sulfotransferase isoform 4 using synthetic oligosaccharide substrates.
Li YJ, Yin FX, Zhang XK, Yu J, Zheng S, Song XL, Wang FS, Sheng JZ
Biochimica et biophysica acta. General subjects1862(3)547-556(2018 Mar)

Genome-wide association studies of maximum number of drinks.
Pan Y, Luo X, Liu X, Wu LY, Zhang Q, Wang L, Wang W, Zuo L, Wang KS
Journal of psychiatric research47(11)1717-24(2013 Nov)

A genome-wide association study (GWAS) for bronchopulmonary dysplasia.
Wang H, St Julien KR, Stevenson DK, Hoffmann TJ, Witte JS, Lazzeroni LC, Krasnow MA, Quaintance CC, Oehlert JW, Jelliffe-Pawlowski LL, Gould JB, Shaw GM, O'Brodovich HM
Pediatrics132(2)290-7(2013 Aug)

NDST4 is a novel candidate tumor suppressor gene at chromosome 4q26 and its genetic loss predicts adverse prognosis in colorectal cancer.
Tzeng ST, Tsai MH, Chen CL, Lee JX, Jao TM, Yu SL, Yen SJ, Yang YC
PloS one8(6)e67040(2013)

Genome-wide association analysis identifies TYW3/CRYZ and NDST4 loci associated with circulating resistin levels.
Qi Q, Menzaghi C, Smith S, Liang L, de Rekeneire N, Garcia ME, Lohman KK, Miljkovic I, Strotmeyer ES, Cummings SR, Kanaya AM, Tylavsky FA, Satterfield S, Ding J, Rimm EB, Trischitta V, Hu FB, Liu Y, Qi L
Human molecular genetics21(21)4774-80(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDST4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDST4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu95377	XM_017008546.1 Latest version!	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu95377	XM_017008545.2 Latest version!	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu25508	NM_022569.3 Latest version!	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$615.30 $879.00
View Old Accession Versions >>
OHu25508	NM_022569.1	Homo sapiens N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (NDST4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$439.50-$615.30 $879.00
OHu95377	XM_017008545.1	Homo sapiens N-deacetylase/N-sulfotransferase 4 (NDST4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu25508	NM_022569.2	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$615.30 $879.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDST4 ( NM_022569.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_072091.1 Homo sapiens N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (NDST4), mRNA.

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CloneID	OHu25508
Clone ID Related Accession (Same CDS sequence)	NM_022569.1 , NM_022569.3 , NM_022569.2
Accession Version	NM_022569.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426262400000
Organism	Homo sapiens(Human)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
Comment

ATGAATCTTA TTGTGAAACT TCGGAGAAGT TTTCGAACAT TGATTGTTCT CTTAGCTACC 
TTTTGCTTGG TGAGCATTGT CATTTCTGCC TATTTTCTCT ACTCTGGCTA CAAACAGGAA 
ATGACACTTA TTGAAACCAC TGCAGAAGCA GAATGCACTG ACATCAAAAT TCTACCATAT 
AGGTCAATGG AGCTGAAAAC AGTTAAACCT ATTGACACAT CCAAAACGGA CCCTACTGTC 
CTTCTCTTCG TGGAGAGCCA ATACTCTCAA CTCGGTCAAG ATATCATAGC TATTTTGGAG 
TCCAGCCGAT TTCAGTACCA CATGGTTATT GCCCCTGGAA AGGGAGATAT ACCTCCTCTT 
ACAGATAATG GCAAAGGGAA ATATACTTTA GTTATTTATG AAAATATTCT GAAGTATGTC 
AGCATGGACT CATGGAATCG AGAGCTTTTA GAAAAATACT GTGTGGAATA CAGTGTTAGT 
ATAATCGGTT TTCATAAAGC CAATGAGAAC AGCTTACCAA GTACACAATT AAAAGGCTTT 
CCGTTAAACC TTTTCAATAA TCTAGCTCTA AAGGACTGTT TTGTTAACCC TCAATCTCCT 
TTGCTGCATA TTACCAAAGC CCCCAAGGTT GAGAAAGGCC CTCTTCCTGG GGAAGACTGG 
ACTATTTTCC AATATAATCA TTCAACCTAC CAGCCTGTAC TTTTAACTGA GTTACAGACA 
GAAAAATCCC TGTCATCCTT GTCTAGCAAA ACACTCTTTG CAACGGTGAT TCAGGATCTG 
GGGCTTCATG ATGGAATTCA GAGAGTACTT TTTGGCAACA ACTTGAACTT TTGGCTGCAC 
AAGCTCATCT TCATAGATGC CATCTCCTTC TTGTCAGGGA AGAGGCTGAC ATTGTCCTTG 
GACAGGTACA TCCTTGTGGA CATTGATGAT ATATTTGTTG GGAAAGAGGG AACAAGGATG 
AATGTCAAAG ATGTGAAGGC ATTACTAGAG ACTCAAAATT TACTGCGCAC TCAGGTTGCA 
AATTTTACCT TCAACCTTGG ATTTTCAGGG AAGTTTTACC ACACAGGGAC TGAAGAGGAA 
GATGAAGGAG ATGACCTTTT ACTTCGGTCT GTGGATGAGT TCTGGTGGTT TCCTCACATG 
TGGAGCCACA TGCAGCCCCA CCTCTTCCAC AACGAGTCAT CTTTAGTAGA GCAGATGATT 
CTCAACAAGG AATTTGCACT GGAACATGGA ATACCAATCA ACATGGGCTA TGCTGTGGCC 
CCACATCACT CAGGGGTCTA CCCGGTTCAC ATTCAGCTGT ATGCAGCTTG GAAGAAGGTC 
TGGGGTATTC AAGTCACCAG CACTGAAGAA TATCCACATC TGAAACCTGC CCGGTACAGA 
AAGGGCTTCA TTCACAATAG CATCATGGTC CTCCCTCGAC AGACTTGTGG GTTGTTCACT 
CACACTATTT TCTACAAAGA ATATCCAGGA GGACCCCAAG AACTGGATAA AAGTATCAGA 
GGAGGTGAAC TTTTTCTCAC AATCCTTCTA AACCCAATCA GCATTTTCAT GACCCATTTA 
TCAAACTATG GAAATGACCG CCTAGGGTTA TATACCTTTG TGAACTTGGT CAACTTTGTG 
CAGAGCTGGA CCAACCTGAA ATTGCAAACT CTGCCTCCAG TGCAACTGGC CCACCAGTAT 
TTTGAGCTCT TCCCTGAGCA GAAAGACCCT CTATGGCAGA ATCCATGTGA TGATAAACGA 
CACAAAGACA TCTGGTCCAG AGAGAAAACT TGTGACCACT TACCAAAATT TCTAGTAATT 
GGTCCACAAA AAACAGGAAC AACTGCACTT TATTTATTTC TTCTTATGCA TCCTTCAATC 
ATCAGCAATC TCCCTAGTCC AAAGACATTT GAGGAAGTTC AGTTCTTTAA TGGCAACAAC 
TATCACAAAG GAATAGACTG GTATATGGAC TTTTTCCCTA CACCATCCAA TACTACAAGT 
GACTTTCTGT TTGAAAAGAG TGCTAATTAC TTCCATTCGG AAGAAGCTCC AAGACGAGCC 
GCATCTCTTG TCCCCAAAGC CAAGATCATC ACCATCCTCA TTGACCCCTC AGACAGGGCA 
TACTCTTGGT ACCAGCACCA ACGATCACAT GAAGATCCAG CTGCTCTGAG GTTCAATTTC 
TATGAAGTTA TTTCAACAGG ACATTGGGCT CCATCTGACT TAAAAACTTT GCAGAGAAGA 
TGCCTAGTAC CTGGATGGTA TGCAGTCCAC ATAGAAAGAT GGCTAACTTA CTTTGCTACT 
TCTCAGTTGC TAATTATTGA TGGACAGCAG CTGAGATCTG ACCCAGCTAC TGTGATGGAT 
GAAGTCCAGA AGTTTCTGGG AGTTACACCT CGTTATAATT ACTCTGAAGC ACTAACGTTT 
GATCCCCAAA AGGGTTTTTG GTGTCAATTA CTGGAAGGAG GAAAGACAAA ATGCCTTGGA 
AAAAGCAAAG GCCGAAAATA TCCACCTATG GATCCAGAGT CTAGAACGTT CCTCTCTAAT 
TACTACCGAG ATCATAATGT GGAACTATCC AAACTGCTAC ACAGACTGGG ACAGCCTCTG 
CCATCGTGGC TGAGACAGGA ACTGCAGAAA GTGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_072091.1

Translation

MNLIVKLRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLIETTAEAECTDIKILPYRSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

Target ORF information:

RefSeq Version	NM_022569.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (NDST4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022569.1

ORF Insert Sequence:

ATGAATCTTA TTGTGAAACT TCGGAGAAGT TTTCGAACAT TGATTGTTCT CTTAGCTACC 
TTTTGCTTGG TGAGCATTGT CATTTCTGCC TATTTTCTCT ACTCTGGCTA CAAACAGGAA 
ATGACACTTA TTGAAACCAC TGCAGAAGCA GAATGCACTG ACATCAAAAT TCTACCATAT 
AGGTCAATGG AGCTGAAAAC AGTTAAACCT ATTGACACAT CCAAAACGGA CCCTACTGTC 
CTTCTCTTCG TGGAGAGCCA ATACTCTCAA CTCGGTCAAG ATATCATAGC TATTTTGGAG 
TCCAGCCGAT TTCAGTACCA CATGGTTATT GCCCCTGGAA AGGGAGATAT ACCTCCTCTT 
ACAGATAATG GCAAAGGGAA ATATACTTTA GTTATTTATG AAAATATTCT GAAGTATGTC 
AGCATGGACT CATGGAATCG AGAGCTTTTA GAAAAATACT GTGTGGAATA CAGTGTTAGT 
ATAATCGGTT TTCATAAAGC CAATGAGAAC AGCTTACCAA GTACACAATT AAAAGGCTTT 
CCGTTAAACC TTTTCAATAA TCTAGCTCTA AAGGACTGTT TTGTTAACCC TCAATCTCCT 
TTGCTGCATA TTACCAAAGC CCCCAAGGTT GAGAAAGGCC CTCTTCCTGG GGAAGACTGG 
ACTATTTTCC AATATAATCA TTCAACCTAC CAGCCTGTAC TTTTAACTGA GTTACAGACA 
GAAAAATCCC TGTCATCCTT GTCTAGCAAA ACACTCTTTG CAACGGTGAT TCAGGATCTG 
GGGCTTCATG ATGGAATTCA GAGAGTACTT TTTGGCAACA ACTTGAACTT TTGGCTGCAC 
AAGCTCATCT TCATAGATGC CATCTCCTTC TTGTCAGGGA AGAGGCTGAC ATTGTCCTTG 
GACAGGTACA TCCTTGTGGA CATTGATGAT ATATTTGTTG GGAAAGAGGG AACAAGGATG 
AATGTCAAAG ATGTGAAGGC ATTACTAGAG ACTCAAAATT TACTGCGCAC TCAGGTTGCA 
AATTTTACCT TCAACCTTGG ATTTTCAGGG AAGTTTTACC ACACAGGGAC TGAAGAGGAA 
GATGAAGGAG ATGACCTTTT ACTTCGGTCT GTGGATGAGT TCTGGTGGTT TCCTCACATG 
TGGAGCCACA TGCAGCCCCA CCTCTTCCAC AACGAGTCAT CTTTAGTAGA GCAGATGATT 
CTCAACAAGG AATTTGCACT GGAACATGGA ATACCAATCA ACATGGGCTA TGCTGTGGCC 
CCACATCACT CAGGGGTCTA CCCGGTTCAC ATTCAGCTGT ATGCAGCTTG GAAGAAGGTC 
TGGGGTATTC AAGTCACCAG CACTGAAGAA TATCCACATC TGAAACCTGC CCGGTACAGA 
AAGGGCTTCA TTCACAATAG CATCATGGTC CTCCCTCGAC AGACTTGTGG GTTGTTCACT 
CACACTATTT TCTACAAAGA ATATCCAGGA GGACCCCAAG AACTGGATAA AAGTATCAGA 
GGAGGTGAAC TTTTTCTCAC AATCCTTCTA AACCCAATCA GCATTTTCAT GACCCATTTA 
TCAAACTATG GAAATGACCG CCTAGGGTTA TATACCTTTG TGAACTTGGT CAACTTTGTG 
CAGAGCTGGA CCAACCTGAA ATTGCAAACT CTGCCTCCAG TGCAACTGGC CCACCAGTAT 
TTTGAGCTCT TCCCTGAGCA GAAAGACCCT CTATGGCAGA ATCCATGTGA TGATAAACGA 
CACAAAGACA TCTGGTCCAG AGAGAAAACT TGTGACCACT TACCAAAATT TCTAGTAATT 
GGTCCACAAA AAACAGGAAC AACTGCACTT TATTTATTTC TTCTTATGCA TCCTTCAATC 
ATCAGCAATC TCCCTAGTCC AAAGACATTT GAGGAAGTTC AGTTCTTTAA TGGCAACAAC 
TATCACAAAG GAATAGACTG GTATATGGAC TTTTTCCCTA CACCATCCAA TACTACAAGT 
GACTTTCTGT TTGAAAAGAG TGCTAATTAC TTCCATTCGG AAGAAGCTCC AAGACGAGCC 
GCATCTCTTG TCCCCAAAGC CAAGATCATC ACCATCCTCA TTGACCCCTC AGACAGGGCA 
TACTCTTGGT ACCAGCACCA ACGATCACAT GAAGATCCAG CTGCTCTGAG GTTCAATTTC 
TATGAAGTTA TTTCAACAGG ACATTGGGCT CCATCTGACT TAAAAACTTT GCAGAGAAGA 
TGCCTAGTAC CTGGATGGTA TGCAGTCCAC ATAGAAAGAT GGCTAACTTA CTTTGCTACT 
TCTCAGTTGC TAATTATTGA TGGACAGCAG CTGAGATCTG ACCCAGCTAC TGTGATGGAT 
GAAGTCCAGA AGTTTCTGGG AGTTACACCT CGTTATAATT ACTCTGAAGC ACTAACGTTT 
GATCCCCAAA AGGGTTTTTG GTGTCAATTA CTGGAAGGAG GAAAGACAAA ATGCCTTGGA 
AAAAGCAAAG GCCGAAAATA TCCACCTATG GATCCAGAGT CTAGAACGTT CCTCTCTAAT 
TACTACCGAG ATCATAATGT GGAACTATCC AAACTGCTAC ACAGACTGGG ACAGCCTCTG 
CCATCGTGGC TGAGACAGGA ACTGCAGAAA GTGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST4 ( XM_017008545.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864034.1 Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95377
Clone ID Related Accession (Same CDS sequence)	XM_017008545.2 , XM_017008546.1 , XM_017008545.1
Accession Version	XM_017008545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGCC ACATGCAGCC CCACCTCTTC CACAACGAGT CATCTTTAGT AGAGCAGATG 
ATTCTCAACA AGGAATTTGC ACTGGAACAT GGAATACCAA TCAACATGGG CTATGCTGTG 
GCCCCACATC ACTCAGGGGT CTACCCGGTT CACATTCAGC TGTATGCAGC TTGGAAGAAG 
GTCTGGGGTA TTCAAGTCAC CAGCACTGAA GAATATCCAC ATCTGAAACC TGCCCGGTAC 
AGAAAGGGCT TCATTCACAA TAGCATCATG GTCCTCCCTC GACAGACTTG TGGGTTGTTC 
ACTCACACTA TTTTCTACAA AGAATATCCA GGAGGACCCC AAGAACTGGA TAAAAGTATC 
AGAGGAGGTG AACTTTTTCT CACAATCCTT CTAAACCCAA TCAGCATTTT CATGACCCAT 
TTATCAAACT ATGGAAATGA CCGCCTAGGG TTATATACCT TTGTGAACTT GGTCAACTTT 
GTGCAGAGCT GGACCAACCT GAAATTGCAA ACTCTGCCTC CAGTGCAACT GGCCCACCAG 
TATTTTGAGC TCTTCCCTGA GCAGAAAGAC CCTCTATGGC AGAATCCATG TGATGATAAA 
CGACACAAAG ACATCTGGTC CAGAGAGAAA ACTTGTGACC ACTTACCAAA ATTTCTAGTA 
ATTGGTCCAC AAAAAACAGG AACAACTGCA CTTTATTTAT TTCTTCTTAT GCATCCTTCA 
ATCATCAGCA ATCTCCCTAG TCCAAAGACA TTTGAGGAAG TTCAGTTCTT TAATGGCAAC 
AACTATCACA AAGGAATAGA CTGGTATATG GACTTTTTCC CTACACCATC CAATACTACA 
AGTGACTTTC TGTTTGAAAA GAGTGCTAAT TACTTCCATT CGGAAGAAGC TCCAAGACGA 
GCCGCATCTC TTGTCCCCAA AGCCAAGATC ATCACCATCC TCATTGACCC CTCAGACAGG 
GCATACTCTT GGTACCAGCA CCAACGATCA CATGAAGATC CAGCTGCTCT GAGGTTCAAT 
TTCTATGAAG TTATTTCAAC AGGACATTGG GCTCCATCTG ACTTAAAAAC TTTGCAGAGA 
AGATGCCTAG TACCTGGATG GTATGCAGTC CACATAGAAA GATGGCTAAC TTACTTTGCT 
ACTTCTCAGT TGCTAATTAT TGATGGACAG CAGCTGAGAT CTGACCCAGC TACTGTGATG 
GATGAAGTCC AGAAGTTTCT GGGAGTTACA CCTCGTTATA ATTACTCTGA AGCACTAACG 
TTTGATCCCC AAAAGGGTTT TTGGTGTCAA TTACTGGAAG GAGGAAAGAC AAAATGCCTT 
GGAAAAAGCA AAGGCCGAAA ATATCCACCT ATGGATCCAG AGTCTAGAAC GTTCCTCTCT 
AATTACTACC GAGATCATAA TGTGGAACTA TCCAAACTGC TACACAGACT GGGACAGCCT 
CTGCCATCGT GGCTGAGACA GGAACTGCAG AAAGTGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864034.1
CDS	850..2331
Translation	MWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

Target ORF information:

RefSeq Version	XM_017008545.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008545.2

ORF Insert Sequence:

ATGTGGAGCC ACATGCAGCC CCACCTCTTC CACAACGAGT CATCTTTAGT AGAGCAGATG 
ATTCTCAACA AGGAATTTGC ACTGGAACAT GGAATACCAA TCAACATGGG CTATGCTGTG 
GCCCCACATC ACTCAGGGGT CTACCCGGTT CACATTCAGC TGTATGCAGC TTGGAAGAAG 
GTCTGGGGTA TTCAAGTCAC CAGCACTGAA GAATATCCAC ATCTGAAACC TGCCCGGTAC 
AGAAAGGGCT TCATTCACAA TAGCATCATG GTCCTCCCTC GACAGACTTG TGGGTTGTTC 
ACTCACACTA TTTTCTACAA AGAATATCCA GGAGGACCCC AAGAACTGGA TAAAAGTATC 
AGAGGAGGTG AACTTTTTCT CACAATCCTT CTAAACCCAA TCAGCATTTT CATGACCCAT 
TTATCAAACT ATGGAAATGA CCGCCTAGGG TTATATACCT TTGTGAACTT GGTCAACTTT 
GTGCAGAGCT GGACCAACCT GAAATTGCAA ACTCTGCCTC CAGTGCAACT GGCCCACCAG 
TATTTTGAGC TCTTCCCTGA GCAGAAAGAC CCTCTATGGC AGAATCCATG TGATGATAAA 
CGACACAAAG ACATCTGGTC CAGAGAGAAA ACTTGTGACC ACTTACCAAA ATTTCTAGTA 
ATTGGTCCAC AAAAAACAGG AACAACTGCA CTTTATTTAT TTCTTCTTAT GCATCCTTCA 
ATCATCAGCA ATCTCCCTAG TCCAAAGACA TTTGAGGAAG TTCAGTTCTT TAATGGCAAC 
AACTATCACA AAGGAATAGA CTGGTATATG GACTTTTTCC CTACACCATC CAATACTACA 
AGTGACTTTC TGTTTGAAAA GAGTGCTAAT TACTTCCATT CGGAAGAAGC TCCAAGACGA 
GCCGCATCTC TTGTCCCCAA AGCCAAGATC ATCACCATCC TCATTGACCC CTCAGACAGG 
GCATACTCTT GGTACCAGCA CCAACGATCA CATGAAGATC CAGCTGCTCT GAGGTTCAAT 
TTCTATGAAG TTATTTCAAC AGGACATTGG GCTCCATCTG ACTTAAAAAC TTTGCAGAGA 
AGATGCCTAG TACCTGGATG GTATGCAGTC CACATAGAAA GATGGCTAAC TTACTTTGCT 
ACTTCTCAGT TGCTAATTAT TGATGGACAG CAGCTGAGAT CTGACCCAGC TACTGTGATG 
GATGAAGTCC AGAAGTTTCT GGGAGTTACA CCTCGTTATA ATTACTCTGA AGCACTAACG 
TTTGATCCCC AAAAGGGTTT TTGGTGTCAA TTACTGGAAG GAGGAAAGAC AAAATGCCTT 
GGAAAAAGCA AAGGCCGAAA ATATCCACCT ATGGATCCAG AGTCTAGAAC GTTCCTCTCT 
AATTACTACC GAGATCATAA TGTGGAACTA TCCAAACTGC TACACAGACT GGGACAGCCT 
CTGCCATCGT GGCTGAGACA GGAACTGCAG AAAGTGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST4 ( XM_017008546.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864035.1 Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), transcript variant X2, mRNA.

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CloneID	OHu95377
Clone ID Related Accession (Same CDS sequence)	XM_017008545.2 , XM_017008546.1 , XM_017008545.1
Accession Version	XM_017008546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGCC ACATGCAGCC CCACCTCTTC CACAACGAGT CATCTTTAGT AGAGCAGATG 
ATTCTCAACA AGGAATTTGC ACTGGAACAT GGAATACCAA TCAACATGGG CTATGCTGTG 
GCCCCACATC ACTCAGGGGT CTACCCGGTT CACATTCAGC TGTATGCAGC TTGGAAGAAG 
GTCTGGGGTA TTCAAGTCAC CAGCACTGAA GAATATCCAC ATCTGAAACC TGCCCGGTAC 
AGAAAGGGCT TCATTCACAA TAGCATCATG GTCCTCCCTC GACAGACTTG TGGGTTGTTC 
ACTCACACTA TTTTCTACAA AGAATATCCA GGAGGACCCC AAGAACTGGA TAAAAGTATC 
AGAGGAGGTG AACTTTTTCT CACAATCCTT CTAAACCCAA TCAGCATTTT CATGACCCAT 
TTATCAAACT ATGGAAATGA CCGCCTAGGG TTATATACCT TTGTGAACTT GGTCAACTTT 
GTGCAGAGCT GGACCAACCT GAAATTGCAA ACTCTGCCTC CAGTGCAACT GGCCCACCAG 
TATTTTGAGC TCTTCCCTGA GCAGAAAGAC CCTCTATGGC AGAATCCATG TGATGATAAA 
CGACACAAAG ACATCTGGTC CAGAGAGAAA ACTTGTGACC ACTTACCAAA ATTTCTAGTA 
ATTGGTCCAC AAAAAACAGG AACAACTGCA CTTTATTTAT TTCTTCTTAT GCATCCTTCA 
ATCATCAGCA ATCTCCCTAG TCCAAAGACA TTTGAGGAAG TTCAGTTCTT TAATGGCAAC 
AACTATCACA AAGGAATAGA CTGGTATATG GACTTTTTCC CTACACCATC CAATACTACA 
AGTGACTTTC TGTTTGAAAA GAGTGCTAAT TACTTCCATT CGGAAGAAGC TCCAAGACGA 
GCCGCATCTC TTGTCCCCAA AGCCAAGATC ATCACCATCC TCATTGACCC CTCAGACAGG 
GCATACTCTT GGTACCAGCA CCAACGATCA CATGAAGATC CAGCTGCTCT GAGGTTCAAT 
TTCTATGAAG TTATTTCAAC AGGACATTGG GCTCCATCTG ACTTAAAAAC TTTGCAGAGA 
AGATGCCTAG TACCTGGATG GTATGCAGTC CACATAGAAA GATGGCTAAC TTACTTTGCT 
ACTTCTCAGT TGCTAATTAT TGATGGACAG CAGCTGAGAT CTGACCCAGC TACTGTGATG 
GATGAAGTCC AGAAGTTTCT GGGAGTTACA CCTCGTTATA ATTACTCTGA AGCACTAACG 
TTTGATCCCC AAAAGGGTTT TTGGTGTCAA TTACTGGAAG GAGGAAAGAC AAAATGCCTT 
GGAAAAAGCA AAGGCCGAAA ATATCCACCT ATGGATCCAG AGTCTAGAAC GTTCCTCTCT 
AATTACTACC GAGATCATAA TGTGGAACTA TCCAAACTGC TACACAGACT GGGACAGCCT 
CTGCCATCGT GGCTGAGACA GGAACTGCAG AAAGTGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864035.1
CDS	121..1602
Translation	MWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

Target ORF information:

RefSeq Version	XM_017008546.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008546.1

ORF Insert Sequence:

ATGTGGAGCC ACATGCAGCC CCACCTCTTC CACAACGAGT CATCTTTAGT AGAGCAGATG 
ATTCTCAACA AGGAATTTGC ACTGGAACAT GGAATACCAA TCAACATGGG CTATGCTGTG 
GCCCCACATC ACTCAGGGGT CTACCCGGTT CACATTCAGC TGTATGCAGC TTGGAAGAAG 
GTCTGGGGTA TTCAAGTCAC CAGCACTGAA GAATATCCAC ATCTGAAACC TGCCCGGTAC 
AGAAAGGGCT TCATTCACAA TAGCATCATG GTCCTCCCTC GACAGACTTG TGGGTTGTTC 
ACTCACACTA TTTTCTACAA AGAATATCCA GGAGGACCCC AAGAACTGGA TAAAAGTATC 
AGAGGAGGTG AACTTTTTCT CACAATCCTT CTAAACCCAA TCAGCATTTT CATGACCCAT 
TTATCAAACT ATGGAAATGA CCGCCTAGGG TTATATACCT TTGTGAACTT GGTCAACTTT 
GTGCAGAGCT GGACCAACCT GAAATTGCAA ACTCTGCCTC CAGTGCAACT GGCCCACCAG 
TATTTTGAGC TCTTCCCTGA GCAGAAAGAC CCTCTATGGC AGAATCCATG TGATGATAAA 
CGACACAAAG ACATCTGGTC CAGAGAGAAA ACTTGTGACC ACTTACCAAA ATTTCTAGTA 
ATTGGTCCAC AAAAAACAGG AACAACTGCA CTTTATTTAT TTCTTCTTAT GCATCCTTCA 
ATCATCAGCA ATCTCCCTAG TCCAAAGACA TTTGAGGAAG TTCAGTTCTT TAATGGCAAC 
AACTATCACA AAGGAATAGA CTGGTATATG GACTTTTTCC CTACACCATC CAATACTACA 
AGTGACTTTC TGTTTGAAAA GAGTGCTAAT TACTTCCATT CGGAAGAAGC TCCAAGACGA 
GCCGCATCTC TTGTCCCCAA AGCCAAGATC ATCACCATCC TCATTGACCC CTCAGACAGG 
GCATACTCTT GGTACCAGCA CCAACGATCA CATGAAGATC CAGCTGCTCT GAGGTTCAAT 
TTCTATGAAG TTATTTCAAC AGGACATTGG GCTCCATCTG ACTTAAAAAC TTTGCAGAGA 
AGATGCCTAG TACCTGGATG GTATGCAGTC CACATAGAAA GATGGCTAAC TTACTTTGCT 
ACTTCTCAGT TGCTAATTAT TGATGGACAG CAGCTGAGAT CTGACCCAGC TACTGTGATG 
GATGAAGTCC AGAAGTTTCT GGGAGTTACA CCTCGTTATA ATTACTCTGA AGCACTAACG 
TTTGATCCCC AAAAGGGTTT TTGGTGTCAA TTACTGGAAG GAGGAAAGAC AAAATGCCTT 
GGAAAAAGCA AAGGCCGAAA ATATCCACCT ATGGATCCAG AGTCTAGAAC GTTCCTCTCT 
AATTACTACC GAGATCATAA TGTGGAACTA TCCAAACTGC TACACAGACT GGGACAGCCT 
CTGCCATCGT GGCTGAGACA GGAACTGCAG AAAGTGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST4 ( XM_017008545.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864034.1 Homo sapiens N-deacetylase/N-sulfotransferase 4 (NDST4), transcript variant X1, mRNA.

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CloneID	OHu95377
Clone ID Related Accession (Same CDS sequence)	XM_017008545.2 , XM_017008546.1 , XM_017008545.1
Accession Version	XM_017008545.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGCC ACATGCAGCC CCACCTCTTC CACAACGAGT CATCTTTAGT AGAGCAGATG 
ATTCTCAACA AGGAATTTGC ACTGGAACAT GGAATACCAA TCAACATGGG CTATGCTGTG 
GCCCCACATC ACTCAGGGGT CTACCCGGTT CACATTCAGC TGTATGCAGC TTGGAAGAAG 
GTCTGGGGTA TTCAAGTCAC CAGCACTGAA GAATATCCAC ATCTGAAACC TGCCCGGTAC 
AGAAAGGGCT TCATTCACAA TAGCATCATG GTCCTCCCTC GACAGACTTG TGGGTTGTTC 
ACTCACACTA TTTTCTACAA AGAATATCCA GGAGGACCCC AAGAACTGGA TAAAAGTATC 
AGAGGAGGTG AACTTTTTCT CACAATCCTT CTAAACCCAA TCAGCATTTT CATGACCCAT 
TTATCAAACT ATGGAAATGA CCGCCTAGGG TTATATACCT TTGTGAACTT GGTCAACTTT 
GTGCAGAGCT GGACCAACCT GAAATTGCAA ACTCTGCCTC CAGTGCAACT GGCCCACCAG 
TATTTTGAGC TCTTCCCTGA GCAGAAAGAC CCTCTATGGC AGAATCCATG TGATGATAAA 
CGACACAAAG ACATCTGGTC CAGAGAGAAA ACTTGTGACC ACTTACCAAA ATTTCTAGTA 
ATTGGTCCAC AAAAAACAGG AACAACTGCA CTTTATTTAT TTCTTCTTAT GCATCCTTCA 
ATCATCAGCA ATCTCCCTAG TCCAAAGACA TTTGAGGAAG TTCAGTTCTT TAATGGCAAC 
AACTATCACA AAGGAATAGA CTGGTATATG GACTTTTTCC CTACACCATC CAATACTACA 
AGTGACTTTC TGTTTGAAAA GAGTGCTAAT TACTTCCATT CGGAAGAAGC TCCAAGACGA 
GCCGCATCTC TTGTCCCCAA AGCCAAGATC ATCACCATCC TCATTGACCC CTCAGACAGG 
GCATACTCTT GGTACCAGCA CCAACGATCA CATGAAGATC CAGCTGCTCT GAGGTTCAAT 
TTCTATGAAG TTATTTCAAC AGGACATTGG GCTCCATCTG ACTTAAAAAC TTTGCAGAGA 
AGATGCCTAG TACCTGGATG GTATGCAGTC CACATAGAAA GATGGCTAAC TTACTTTGCT 
ACTTCTCAGT TGCTAATTAT TGATGGACAG CAGCTGAGAT CTGACCCAGC TACTGTGATG 
GATGAAGTCC AGAAGTTTCT GGGAGTTACA CCTCGTTATA ATTACTCTGA AGCACTAACG 
TTTGATCCCC AAAAGGGTTT TTGGTGTCAA TTACTGGAAG GAGGAAAGAC AAAATGCCTT 
GGAAAAAGCA AAGGCCGAAA ATATCCACCT ATGGATCCAG AGTCTAGAAC GTTCCTCTCT 
AATTACTACC GAGATCATAA TGTGGAACTA TCCAAACTGC TACACAGACT GGGACAGCCT 
CTGCCATCGT GGCTGAGACA GGAACTGCAG AAAGTGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864034.1
CDS	815..2296
Translation	MWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

Target ORF information:

RefSeq Version	XM_017008545.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-deacetylase/N-sulfotransferase 4 (NDST4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008545.1

ORF Insert Sequence:

ATGTGGAGCC ACATGCAGCC CCACCTCTTC CACAACGAGT CATCTTTAGT AGAGCAGATG 
ATTCTCAACA AGGAATTTGC ACTGGAACAT GGAATACCAA TCAACATGGG CTATGCTGTG 
GCCCCACATC ACTCAGGGGT CTACCCGGTT CACATTCAGC TGTATGCAGC TTGGAAGAAG 
GTCTGGGGTA TTCAAGTCAC CAGCACTGAA GAATATCCAC ATCTGAAACC TGCCCGGTAC 
AGAAAGGGCT TCATTCACAA TAGCATCATG GTCCTCCCTC GACAGACTTG TGGGTTGTTC 
ACTCACACTA TTTTCTACAA AGAATATCCA GGAGGACCCC AAGAACTGGA TAAAAGTATC 
AGAGGAGGTG AACTTTTTCT CACAATCCTT CTAAACCCAA TCAGCATTTT CATGACCCAT 
TTATCAAACT ATGGAAATGA CCGCCTAGGG TTATATACCT TTGTGAACTT GGTCAACTTT 
GTGCAGAGCT GGACCAACCT GAAATTGCAA ACTCTGCCTC CAGTGCAACT GGCCCACCAG 
TATTTTGAGC TCTTCCCTGA GCAGAAAGAC CCTCTATGGC AGAATCCATG TGATGATAAA 
CGACACAAAG ACATCTGGTC CAGAGAGAAA ACTTGTGACC ACTTACCAAA ATTTCTAGTA 
ATTGGTCCAC AAAAAACAGG AACAACTGCA CTTTATTTAT TTCTTCTTAT GCATCCTTCA 
ATCATCAGCA ATCTCCCTAG TCCAAAGACA TTTGAGGAAG TTCAGTTCTT TAATGGCAAC 
AACTATCACA AAGGAATAGA CTGGTATATG GACTTTTTCC CTACACCATC CAATACTACA 
AGTGACTTTC TGTTTGAAAA GAGTGCTAAT TACTTCCATT CGGAAGAAGC TCCAAGACGA 
GCCGCATCTC TTGTCCCCAA AGCCAAGATC ATCACCATCC TCATTGACCC CTCAGACAGG 
GCATACTCTT GGTACCAGCA CCAACGATCA CATGAAGATC CAGCTGCTCT GAGGTTCAAT 
TTCTATGAAG TTATTTCAAC AGGACATTGG GCTCCATCTG ACTTAAAAAC TTTGCAGAGA 
AGATGCCTAG TACCTGGATG GTATGCAGTC CACATAGAAA GATGGCTAAC TTACTTTGCT 
ACTTCTCAGT TGCTAATTAT TGATGGACAG CAGCTGAGAT CTGACCCAGC TACTGTGATG 
GATGAAGTCC AGAAGTTTCT GGGAGTTACA CCTCGTTATA ATTACTCTGA AGCACTAACG 
TTTGATCCCC AAAAGGGTTT TTGGTGTCAA TTACTGGAAG GAGGAAAGAC AAAATGCCTT 
GGAAAAAGCA AAGGCCGAAA ATATCCACCT ATGGATCCAG AGTCTAGAAC GTTCCTCTCT 
AATTACTACC GAGATCATAA TGTGGAACTA TCCAAACTGC TACACAGACT GGGACAGCCT 
CTGCCATCGT GGCTGAGACA GGAACTGCAG AAAGTGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST4 ( NM_022569.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_072091.1 Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), mRNA.

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CloneID	OHu25508
Clone ID Related Accession (Same CDS sequence)	NM_022569.1 , NM_022569.3 , NM_022569.2
Accession Version	NM_022569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB036429.1, BC012326.1, AK289670.1 and AC110777.3. On May 17, 2019 this sequence version replaced NM_022569.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.260201.1, SRR1803612.146520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMN03267773 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264363.7/ ENSP00000264363.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGAATCTTA TTGTGAAACT TCGGAGAAGT TTTCGAACAT TGATTGTTCT CTTAGCTACC 
TTTTGCTTGG TGAGCATTGT CATTTCTGCC TATTTTCTCT ACTCTGGCTA CAAACAGGAA 
ATGACACTTA TTGAAACCAC TGCAGAAGCA GAATGCACTG ACATCAAAAT TCTACCATAT 
AGGTCAATGG AGCTGAAAAC AGTTAAACCT ATTGACACAT CCAAAACGGA CCCTACTGTC 
CTTCTCTTCG TGGAGAGCCA ATACTCTCAA CTCGGTCAAG ATATCATAGC TATTTTGGAG 
TCCAGCCGAT TTCAGTACCA CATGGTTATT GCCCCTGGAA AGGGAGATAT ACCTCCTCTT 
ACAGATAATG GCAAAGGGAA ATATACTTTA GTTATTTATG AAAATATTCT GAAGTATGTC 
AGCATGGACT CATGGAATCG AGAGCTTTTA GAAAAATACT GTGTGGAATA CAGTGTTAGT 
ATAATCGGTT TTCATAAAGC CAATGAGAAC AGCTTACCAA GTACACAATT AAAAGGCTTT 
CCGTTAAACC TTTTCAATAA TCTAGCTCTA AAGGACTGTT TTGTTAACCC TCAATCTCCT 
TTGCTGCATA TTACCAAAGC CCCCAAGGTT GAGAAAGGCC CTCTTCCTGG GGAAGACTGG 
ACTATTTTCC AATATAATCA TTCAACCTAC CAGCCTGTAC TTTTAACTGA GTTACAGACA 
GAAAAATCCC TGTCATCCTT GTCTAGCAAA ACACTCTTTG CAACGGTGAT TCAGGATCTG 
GGGCTTCATG ATGGAATTCA GAGAGTACTT TTTGGCAACA ACTTGAACTT TTGGCTGCAC 
AAGCTCATCT TCATAGATGC CATCTCCTTC TTGTCAGGGA AGAGGCTGAC ATTGTCCTTG 
GACAGGTACA TCCTTGTGGA CATTGATGAT ATATTTGTTG GGAAAGAGGG AACAAGGATG 
AATGTCAAAG ATGTGAAGGC ATTACTAGAG ACTCAAAATT TACTGCGCAC TCAGGTTGCA 
AATTTTACCT TCAACCTTGG ATTTTCAGGG AAGTTTTACC ACACAGGGAC TGAAGAGGAA 
GATGAAGGAG ATGACCTTTT ACTTCGGTCT GTGGATGAGT TCTGGTGGTT TCCTCACATG 
TGGAGCCACA TGCAGCCCCA CCTCTTCCAC AACGAGTCAT CTTTAGTAGA GCAGATGATT 
CTCAACAAGG AATTTGCACT GGAACATGGA ATACCAATCA ACATGGGCTA TGCTGTGGCC 
CCACATCACT CAGGGGTCTA CCCGGTTCAC ATTCAGCTGT ATGCAGCTTG GAAGAAGGTC 
TGGGGTATTC AAGTCACCAG CACTGAAGAA TATCCACATC TGAAACCTGC CCGGTACAGA 
AAGGGCTTCA TTCACAATAG CATCATGGTC CTCCCTCGAC AGACTTGTGG GTTGTTCACT 
CACACTATTT TCTACAAAGA ATATCCAGGA GGACCCCAAG AACTGGATAA AAGTATCAGA 
GGAGGTGAAC TTTTTCTCAC AATCCTTCTA AACCCAATCA GCATTTTCAT GACCCATTTA 
TCAAACTATG GAAATGACCG CCTAGGGTTA TATACCTTTG TGAACTTGGT CAACTTTGTG 
CAGAGCTGGA CCAACCTGAA ATTGCAAACT CTGCCTCCAG TGCAACTGGC CCACCAGTAT 
TTTGAGCTCT TCCCTGAGCA GAAAGACCCT CTATGGCAGA ATCCATGTGA TGATAAACGA 
CACAAAGACA TCTGGTCCAG AGAGAAAACT TGTGACCACT TACCAAAATT TCTAGTAATT 
GGTCCACAAA AAACAGGAAC AACTGCACTT TATTTATTTC TTCTTATGCA TCCTTCAATC 
ATCAGCAATC TCCCTAGTCC AAAGACATTT GAGGAAGTTC AGTTCTTTAA TGGCAACAAC 
TATCACAAAG GAATAGACTG GTATATGGAC TTTTTCCCTA CACCATCCAA TACTACAAGT 
GACTTTCTGT TTGAAAAGAG TGCTAATTAC TTCCATTCGG AAGAAGCTCC AAGACGAGCC 
GCATCTCTTG TCCCCAAAGC CAAGATCATC ACCATCCTCA TTGACCCCTC AGACAGGGCA 
TACTCTTGGT ACCAGCACCA ACGATCACAT GAAGATCCAG CTGCTCTGAG GTTCAATTTC 
TATGAAGTTA TTTCAACAGG ACATTGGGCT CCATCTGACT TAAAAACTTT GCAGAGAAGA 
TGCCTAGTAC CTGGATGGTA TGCAGTCCAC ATAGAAAGAT GGCTAACTTA CTTTGCTACT 
TCTCAGTTGC TAATTATTGA TGGACAGCAG CTGAGATCTG ACCCAGCTAC TGTGATGGAT 
GAAGTCCAGA AGTTTCTGGG AGTTACACCT CGTTATAATT ACTCTGAAGC ACTAACGTTT 
GATCCCCAAA AGGGTTTTTG GTGTCAATTA CTGGAAGGAG GAAAGACAAA ATGCCTTGGA 
AAAAGCAAAG GCCGAAAATA TCCACCTATG GATCCAGAGT CTAGAACGTT CCTCTCTAAT 
TACTACCGAG ATCATAATGT GGAACTATCC AAACTGCTAC ACAGACTGGG ACAGCCTCTG 
CCATCGTGGC TGAGACAGGA ACTGCAGAAA GTGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072091.1
CDS	424..3042
Translation	MNLIVKLRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLIETTAEAECTDIKILPYRSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

Target ORF information:

RefSeq Version	NM_022569.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022569.3

ORF Insert Sequence:

ATGAATCTTA TTGTGAAACT TCGGAGAAGT TTTCGAACAT TGATTGTTCT CTTAGCTACC 
TTTTGCTTGG TGAGCATTGT CATTTCTGCC TATTTTCTCT ACTCTGGCTA CAAACAGGAA 
ATGACACTTA TTGAAACCAC TGCAGAAGCA GAATGCACTG ACATCAAAAT TCTACCATAT 
AGGTCAATGG AGCTGAAAAC AGTTAAACCT ATTGACACAT CCAAAACGGA CCCTACTGTC 
CTTCTCTTCG TGGAGAGCCA ATACTCTCAA CTCGGTCAAG ATATCATAGC TATTTTGGAG 
TCCAGCCGAT TTCAGTACCA CATGGTTATT GCCCCTGGAA AGGGAGATAT ACCTCCTCTT 
ACAGATAATG GCAAAGGGAA ATATACTTTA GTTATTTATG AAAATATTCT GAAGTATGTC 
AGCATGGACT CATGGAATCG AGAGCTTTTA GAAAAATACT GTGTGGAATA CAGTGTTAGT 
ATAATCGGTT TTCATAAAGC CAATGAGAAC AGCTTACCAA GTACACAATT AAAAGGCTTT 
CCGTTAAACC TTTTCAATAA TCTAGCTCTA AAGGACTGTT TTGTTAACCC TCAATCTCCT 
TTGCTGCATA TTACCAAAGC CCCCAAGGTT GAGAAAGGCC CTCTTCCTGG GGAAGACTGG 
ACTATTTTCC AATATAATCA TTCAACCTAC CAGCCTGTAC TTTTAACTGA GTTACAGACA 
GAAAAATCCC TGTCATCCTT GTCTAGCAAA ACACTCTTTG CAACGGTGAT TCAGGATCTG 
GGGCTTCATG ATGGAATTCA GAGAGTACTT TTTGGCAACA ACTTGAACTT TTGGCTGCAC 
AAGCTCATCT TCATAGATGC CATCTCCTTC TTGTCAGGGA AGAGGCTGAC ATTGTCCTTG 
GACAGGTACA TCCTTGTGGA CATTGATGAT ATATTTGTTG GGAAAGAGGG AACAAGGATG 
AATGTCAAAG ATGTGAAGGC ATTACTAGAG ACTCAAAATT TACTGCGCAC TCAGGTTGCA 
AATTTTACCT TCAACCTTGG ATTTTCAGGG AAGTTTTACC ACACAGGGAC TGAAGAGGAA 
GATGAAGGAG ATGACCTTTT ACTTCGGTCT GTGGATGAGT TCTGGTGGTT TCCTCACATG 
TGGAGCCACA TGCAGCCCCA CCTCTTCCAC AACGAGTCAT CTTTAGTAGA GCAGATGATT 
CTCAACAAGG AATTTGCACT GGAACATGGA ATACCAATCA ACATGGGCTA TGCTGTGGCC 
CCACATCACT CAGGGGTCTA CCCGGTTCAC ATTCAGCTGT ATGCAGCTTG GAAGAAGGTC 
TGGGGTATTC AAGTCACCAG CACTGAAGAA TATCCACATC TGAAACCTGC CCGGTACAGA 
AAGGGCTTCA TTCACAATAG CATCATGGTC CTCCCTCGAC AGACTTGTGG GTTGTTCACT 
CACACTATTT TCTACAAAGA ATATCCAGGA GGACCCCAAG AACTGGATAA AAGTATCAGA 
GGAGGTGAAC TTTTTCTCAC AATCCTTCTA AACCCAATCA GCATTTTCAT GACCCATTTA 
TCAAACTATG GAAATGACCG CCTAGGGTTA TATACCTTTG TGAACTTGGT CAACTTTGTG 
CAGAGCTGGA CCAACCTGAA ATTGCAAACT CTGCCTCCAG TGCAACTGGC CCACCAGTAT 
TTTGAGCTCT TCCCTGAGCA GAAAGACCCT CTATGGCAGA ATCCATGTGA TGATAAACGA 
CACAAAGACA TCTGGTCCAG AGAGAAAACT TGTGACCACT TACCAAAATT TCTAGTAATT 
GGTCCACAAA AAACAGGAAC AACTGCACTT TATTTATTTC TTCTTATGCA TCCTTCAATC 
ATCAGCAATC TCCCTAGTCC AAAGACATTT GAGGAAGTTC AGTTCTTTAA TGGCAACAAC 
TATCACAAAG GAATAGACTG GTATATGGAC TTTTTCCCTA CACCATCCAA TACTACAAGT 
GACTTTCTGT TTGAAAAGAG TGCTAATTAC TTCCATTCGG AAGAAGCTCC AAGACGAGCC 
GCATCTCTTG TCCCCAAAGC CAAGATCATC ACCATCCTCA TTGACCCCTC AGACAGGGCA 
TACTCTTGGT ACCAGCACCA ACGATCACAT GAAGATCCAG CTGCTCTGAG GTTCAATTTC 
TATGAAGTTA TTTCAACAGG ACATTGGGCT CCATCTGACT TAAAAACTTT GCAGAGAAGA 
TGCCTAGTAC CTGGATGGTA TGCAGTCCAC ATAGAAAGAT GGCTAACTTA CTTTGCTACT 
TCTCAGTTGC TAATTATTGA TGGACAGCAG CTGAGATCTG ACCCAGCTAC TGTGATGGAT 
GAAGTCCAGA AGTTTCTGGG AGTTACACCT CGTTATAATT ACTCTGAAGC ACTAACGTTT 
GATCCCCAAA AGGGTTTTTG GTGTCAATTA CTGGAAGGAG GAAAGACAAA ATGCCTTGGA 
AAAAGCAAAG GCCGAAAATA TCCACCTATG GATCCAGAGT CTAGAACGTT CCTCTCTAAT 
TACTACCGAG ATCATAATGT GGAACTATCC AAACTGCTAC ACAGACTGGG ACAGCCTCTG 
CCATCGTGGC TGAGACAGGA ACTGCAGAAA GTGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST4 ( NM_022569.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_072091.1 Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), mRNA.

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CloneID	OHu25508
Clone ID Related Accession (Same CDS sequence)	NM_022569.1 , NM_022569.3 , NM_022569.2
Accession Version	NM_022569.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550937600000
Organism	Homo sapiens(human)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB036429.1, BC012326.1 and AK289670.1. On Dec 9, 2015 this sequence version replaced NM_022569.1. ##Evidence-Data-START## Transcript exon combination :: AB036429.1, SRR1803613.260201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMN03267773 [ECO:0000348] ##Evidence-Data-END##

ATGAATCTTA TTGTGAAACT TCGGAGAAGT TTTCGAACAT TGATTGTTCT CTTAGCTACC 
TTTTGCTTGG TGAGCATTGT CATTTCTGCC TATTTTCTCT ACTCTGGCTA CAAACAGGAA 
ATGACACTTA TTGAAACCAC TGCAGAAGCA GAATGCACTG ACATCAAAAT TCTACCATAT 
AGGTCAATGG AGCTGAAAAC AGTTAAACCT ATTGACACAT CCAAAACGGA CCCTACTGTC 
CTTCTCTTCG TGGAGAGCCA ATACTCTCAA CTCGGTCAAG ATATCATAGC TATTTTGGAG 
TCCAGCCGAT TTCAGTACCA CATGGTTATT GCCCCTGGAA AGGGAGATAT ACCTCCTCTT 
ACAGATAATG GCAAAGGGAA ATATACTTTA GTTATTTATG AAAATATTCT GAAGTATGTC 
AGCATGGACT CATGGAATCG AGAGCTTTTA GAAAAATACT GTGTGGAATA CAGTGTTAGT 
ATAATCGGTT TTCATAAAGC CAATGAGAAC AGCTTACCAA GTACACAATT AAAAGGCTTT 
CCGTTAAACC TTTTCAATAA TCTAGCTCTA AAGGACTGTT TTGTTAACCC TCAATCTCCT 
TTGCTGCATA TTACCAAAGC CCCCAAGGTT GAGAAAGGCC CTCTTCCTGG GGAAGACTGG 
ACTATTTTCC AATATAATCA TTCAACCTAC CAGCCTGTAC TTTTAACTGA GTTACAGACA 
GAAAAATCCC TGTCATCCTT GTCTAGCAAA ACACTCTTTG CAACGGTGAT TCAGGATCTG 
GGGCTTCATG ATGGAATTCA GAGAGTACTT TTTGGCAACA ACTTGAACTT TTGGCTGCAC 
AAGCTCATCT TCATAGATGC CATCTCCTTC TTGTCAGGGA AGAGGCTGAC ATTGTCCTTG 
GACAGGTACA TCCTTGTGGA CATTGATGAT ATATTTGTTG GGAAAGAGGG AACAAGGATG 
AATGTCAAAG ATGTGAAGGC ATTACTAGAG ACTCAAAATT TACTGCGCAC TCAGGTTGCA 
AATTTTACCT TCAACCTTGG ATTTTCAGGG AAGTTTTACC ACACAGGGAC TGAAGAGGAA 
GATGAAGGAG ATGACCTTTT ACTTCGGTCT GTGGATGAGT TCTGGTGGTT TCCTCACATG 
TGGAGCCACA TGCAGCCCCA CCTCTTCCAC AACGAGTCAT CTTTAGTAGA GCAGATGATT 
CTCAACAAGG AATTTGCACT GGAACATGGA ATACCAATCA ACATGGGCTA TGCTGTGGCC 
CCACATCACT CAGGGGTCTA CCCGGTTCAC ATTCAGCTGT ATGCAGCTTG GAAGAAGGTC 
TGGGGTATTC AAGTCACCAG CACTGAAGAA TATCCACATC TGAAACCTGC CCGGTACAGA 
AAGGGCTTCA TTCACAATAG CATCATGGTC CTCCCTCGAC AGACTTGTGG GTTGTTCACT 
CACACTATTT TCTACAAAGA ATATCCAGGA GGACCCCAAG AACTGGATAA AAGTATCAGA 
GGAGGTGAAC TTTTTCTCAC AATCCTTCTA AACCCAATCA GCATTTTCAT GACCCATTTA 
TCAAACTATG GAAATGACCG CCTAGGGTTA TATACCTTTG TGAACTTGGT CAACTTTGTG 
CAGAGCTGGA CCAACCTGAA ATTGCAAACT CTGCCTCCAG TGCAACTGGC CCACCAGTAT 
TTTGAGCTCT TCCCTGAGCA GAAAGACCCT CTATGGCAGA ATCCATGTGA TGATAAACGA 
CACAAAGACA TCTGGTCCAG AGAGAAAACT TGTGACCACT TACCAAAATT TCTAGTAATT 
GGTCCACAAA AAACAGGAAC AACTGCACTT TATTTATTTC TTCTTATGCA TCCTTCAATC 
ATCAGCAATC TCCCTAGTCC AAAGACATTT GAGGAAGTTC AGTTCTTTAA TGGCAACAAC 
TATCACAAAG GAATAGACTG GTATATGGAC TTTTTCCCTA CACCATCCAA TACTACAAGT 
GACTTTCTGT TTGAAAAGAG TGCTAATTAC TTCCATTCGG AAGAAGCTCC AAGACGAGCC 
GCATCTCTTG TCCCCAAAGC CAAGATCATC ACCATCCTCA TTGACCCCTC AGACAGGGCA 
TACTCTTGGT ACCAGCACCA ACGATCACAT GAAGATCCAG CTGCTCTGAG GTTCAATTTC 
TATGAAGTTA TTTCAACAGG ACATTGGGCT CCATCTGACT TAAAAACTTT GCAGAGAAGA 
TGCCTAGTAC CTGGATGGTA TGCAGTCCAC ATAGAAAGAT GGCTAACTTA CTTTGCTACT 
TCTCAGTTGC TAATTATTGA TGGACAGCAG CTGAGATCTG ACCCAGCTAC TGTGATGGAT 
GAAGTCCAGA AGTTTCTGGG AGTTACACCT CGTTATAATT ACTCTGAAGC ACTAACGTTT 
GATCCCCAAA AGGGTTTTTG GTGTCAATTA CTGGAAGGAG GAAAGACAAA ATGCCTTGGA 
AAAAGCAAAG GCCGAAAATA TCCACCTATG GATCCAGAGT CTAGAACGTT CCTCTCTAAT 
TACTACCGAG ATCATAATGT GGAACTATCC AAACTGCTAC ACAGACTGGG ACAGCCTCTG 
CCATCGTGGC TGAGACAGGA ACTGCAGAAA GTGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072091.1
CDS	680..3298
Translation	MNLIVKLRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLIETTAEAECTDIKILPYRSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVAPHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSNYYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

Target ORF information:

RefSeq Version	NM_022569.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-deacetylase and N-sulfotransferase 4 (NDST4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022569.2

ORF Insert Sequence:

ATGAATCTTA TTGTGAAACT TCGGAGAAGT TTTCGAACAT TGATTGTTCT CTTAGCTACC 
TTTTGCTTGG TGAGCATTGT CATTTCTGCC TATTTTCTCT ACTCTGGCTA CAAACAGGAA 
ATGACACTTA TTGAAACCAC TGCAGAAGCA GAATGCACTG ACATCAAAAT TCTACCATAT 
AGGTCAATGG AGCTGAAAAC AGTTAAACCT ATTGACACAT CCAAAACGGA CCCTACTGTC 
CTTCTCTTCG TGGAGAGCCA ATACTCTCAA CTCGGTCAAG ATATCATAGC TATTTTGGAG 
TCCAGCCGAT TTCAGTACCA CATGGTTATT GCCCCTGGAA AGGGAGATAT ACCTCCTCTT 
ACAGATAATG GCAAAGGGAA ATATACTTTA GTTATTTATG AAAATATTCT GAAGTATGTC 
AGCATGGACT CATGGAATCG AGAGCTTTTA GAAAAATACT GTGTGGAATA CAGTGTTAGT 
ATAATCGGTT TTCATAAAGC CAATGAGAAC AGCTTACCAA GTACACAATT AAAAGGCTTT 
CCGTTAAACC TTTTCAATAA TCTAGCTCTA AAGGACTGTT TTGTTAACCC TCAATCTCCT 
TTGCTGCATA TTACCAAAGC CCCCAAGGTT GAGAAAGGCC CTCTTCCTGG GGAAGACTGG 
ACTATTTTCC AATATAATCA TTCAACCTAC CAGCCTGTAC TTTTAACTGA GTTACAGACA 
GAAAAATCCC TGTCATCCTT GTCTAGCAAA ACACTCTTTG CAACGGTGAT TCAGGATCTG 
GGGCTTCATG ATGGAATTCA GAGAGTACTT TTTGGCAACA ACTTGAACTT TTGGCTGCAC 
AAGCTCATCT TCATAGATGC CATCTCCTTC TTGTCAGGGA AGAGGCTGAC ATTGTCCTTG 
GACAGGTACA TCCTTGTGGA CATTGATGAT ATATTTGTTG GGAAAGAGGG AACAAGGATG 
AATGTCAAAG ATGTGAAGGC ATTACTAGAG ACTCAAAATT TACTGCGCAC TCAGGTTGCA 
AATTTTACCT TCAACCTTGG ATTTTCAGGG AAGTTTTACC ACACAGGGAC TGAAGAGGAA 
GATGAAGGAG ATGACCTTTT ACTTCGGTCT GTGGATGAGT TCTGGTGGTT TCCTCACATG 
TGGAGCCACA TGCAGCCCCA CCTCTTCCAC AACGAGTCAT CTTTAGTAGA GCAGATGATT 
CTCAACAAGG AATTTGCACT GGAACATGGA ATACCAATCA ACATGGGCTA TGCTGTGGCC 
CCACATCACT CAGGGGTCTA CCCGGTTCAC ATTCAGCTGT ATGCAGCTTG GAAGAAGGTC 
TGGGGTATTC AAGTCACCAG CACTGAAGAA TATCCACATC TGAAACCTGC CCGGTACAGA 
AAGGGCTTCA TTCACAATAG CATCATGGTC CTCCCTCGAC AGACTTGTGG GTTGTTCACT 
CACACTATTT TCTACAAAGA ATATCCAGGA GGACCCCAAG AACTGGATAA AAGTATCAGA 
GGAGGTGAAC TTTTTCTCAC AATCCTTCTA AACCCAATCA GCATTTTCAT GACCCATTTA 
TCAAACTATG GAAATGACCG CCTAGGGTTA TATACCTTTG TGAACTTGGT CAACTTTGTG 
CAGAGCTGGA CCAACCTGAA ATTGCAAACT CTGCCTCCAG TGCAACTGGC CCACCAGTAT 
TTTGAGCTCT TCCCTGAGCA GAAAGACCCT CTATGGCAGA ATCCATGTGA TGATAAACGA 
CACAAAGACA TCTGGTCCAG AGAGAAAACT TGTGACCACT TACCAAAATT TCTAGTAATT 
GGTCCACAAA AAACAGGAAC AACTGCACTT TATTTATTTC TTCTTATGCA TCCTTCAATC 
ATCAGCAATC TCCCTAGTCC AAAGACATTT GAGGAAGTTC AGTTCTTTAA TGGCAACAAC 
TATCACAAAG GAATAGACTG GTATATGGAC TTTTTCCCTA CACCATCCAA TACTACAAGT 
GACTTTCTGT TTGAAAAGAG TGCTAATTAC TTCCATTCGG AAGAAGCTCC AAGACGAGCC 
GCATCTCTTG TCCCCAAAGC CAAGATCATC ACCATCCTCA TTGACCCCTC AGACAGGGCA 
TACTCTTGGT ACCAGCACCA ACGATCACAT GAAGATCCAG CTGCTCTGAG GTTCAATTTC 
TATGAAGTTA TTTCAACAGG ACATTGGGCT CCATCTGACT TAAAAACTTT GCAGAGAAGA 
TGCCTAGTAC CTGGATGGTA TGCAGTCCAC ATAGAAAGAT GGCTAACTTA CTTTGCTACT 
TCTCAGTTGC TAATTATTGA TGGACAGCAG CTGAGATCTG ACCCAGCTAC TGTGATGGAT 
GAAGTCCAGA AGTTTCTGGG AGTTACACCT CGTTATAATT ACTCTGAAGC ACTAACGTTT 
GATCCCCAAA AGGGTTTTTG GTGTCAATTA CTGGAAGGAG GAAAGACAAA ATGCCTTGGA 
AAAAGCAAAG GCCGAAAATA TCCACCTATG GATCCAGAGT CTAGAACGTT CCTCTCTAAT 
TACTACCGAG ATCATAATGT GGAACTATCC AAACTGCTAC ACAGACTGGG ACAGCCTCTG 
CCATCGTGGC TGAGACAGGA ACTGCAGAAA GTGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of heparan sulfate N-deacetylase/N-sulfotransferase isoform 4 using synthetic oligosaccharide substrates.
Biochimica et biophysica acta. General subjects1862(3)547-556(2018 Mar)
Li YJ,Yin FX,Zhang XK,Yu J,Zheng S,Song XL,Wang FS,Sheng JZ

Genome-wide association studies of maximum number of drinks.
Journal of psychiatric research47(11)1717-24(2013 Nov)
Pan Y,Luo X,Liu X,Wu LY,Zhang Q,Wang L,Wang W,Zuo L,Wang KS

A genome-wide association study (GWAS) for bronchopulmonary dysplasia.
Pediatrics132(2)290-7(2013 Aug)
Wang H,St Julien KR,Stevenson DK,Hoffmann TJ,Witte JS,Lazzeroni LC,Krasnow MA,Quaintance CC,Oehlert JW,Jelliffe-Pawlowski LL,Gould JB,Shaw GM,O'Brodovich HM

NDST4 is a novel candidate tumor suppressor gene at chromosome 4q26 and its genetic loss predicts adverse prognosis in colorectal cancer.
PloS one8(6)e67040(2013)
Tzeng ST,Tsai MH,Chen CL,Lee JX,Jao TM,Yu SL,Yen SJ,Yang YC

Genome-wide association analysis identifies TYW3/CRYZ and NDST4 loci associated with circulating resistin levels.
Human molecular genetics21(21)4774-80(2012 Nov)
Qi Q,Menzaghi C,Smith S,Liang L,de Rekeneire N,Garcia ME,Lohman KK,Miljkovic I,Strotmeyer ES,Cummings SR,Kanaya AM,Tylavsky FA,Satterfield S,Ding J,Rimm EB,Trischitta V,Hu FB,Liu Y,Qi L

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NDST4 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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