RNF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF4
Entrez Gene ID	6047
Full Name	ring finger protein 4
Synonyms	RES4-26,SLX5,SNURF
General protein information	Preferred Names ring finger protein 4 Names E3 ubiquitin-protein ligase RNF4 E3 ubiquitin ligase RNF4 RING-type E3 ubiquitin transferase RNF4 small nuclear RING finger protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Summary	The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010].
Disorder MIM:	602850

mRNA and Protein(s)

mRNA	Protein	Name
NM_001185010.3	NP_001171939.1	E3 ubiquitin-protein ligase RNF4 isoform 2
NM_002938.5	NP_002929.1	E3 ubiquitin-protein ligase RNF4 isoform 1
NM_001185010.2	NP_001171939.1	E3 ubiquitin-protein ligase RNF4 isoform 2
NM_001185009.2	NP_001171938.1	E3 ubiquitin-protein ligase RNF4 isoform 1
NM_002938.4	NP_002929.1	E3 ubiquitin-protein ligase RNF4 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF4	NP_001171938.1
Danio rerio (zebrafish)	im:7142942	XP_683428.1
Xenopus tropicalis (tropical clawed frog)	rnf4	NP_001016554.1
Pan troglodytes (chimpanzee)	RNF4	NP_001238838.1
Canis lupus familiaris (dog)	RNF4	XP_853173.1
Mus musculus (house mouse)	Rnf4	NP_035408.1
Bos taurus (cattle)	RNF4	NP_001040054.1
Rattus norvegicus (Norway rat)	Rnf4	NP_062055.1
Gallus gallus (chicken)	RNF4	NP_001012907.1

Gene Ontology
Bibliography

Related articles in PubMed

SUMOylation by SUMO2 is implicated in the degradation of misfolded ataxin-7 via RNF4 in SCA7 models.
Marinello M, Werner A, Giannone M, Tahiri K, Alves S, Tesson C, den Dunnen W, Seeler JS, Brice A, Sittler A
Disease models & mechanisms12(1)(2019 01)

RNF4 regulates zebrafish granulopoiesis through the DNMT1-C/EBPα axis.
Wang L, Liu X, Wang H, Yuan H, Chen S, Chen Z, de The H, Zhou J, Zhu J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology32(9)4930-4940(2018 09)

E1B-55K-Mediated Regulation of RNF4 SUMO-Targeted Ubiquitin Ligase Promotes Human Adenovirus Gene Expression.
Müncheberg S, Hay RT, Ip WH, Meyer T, Weiß C, Brenke J, Masser S, Hadian K, Dobner T, Schreiner S
Journal of virology92(13)(2018 07)

Respiratory syncytial virus induces NRF2 degradation through a promyelocytic leukemia protein - ring finger protein 4 dependent pathway.
Komaravelli N, Ansar M, Garofalo RP, Casola A
Free radical biology & medicine113494-504(2017 12)

The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery.
Kumar R, González-Prieto R, Xiao Z, Verlaan-de Vries M, Vertegaal ACO
Nature communications8(1)1809(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RNF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10761	NM_002938.5 Latest version!	Homo sapiens ring finger protein 4 (RNF4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10761	NM_001185009.2 Latest version!	Homo sapiens ring finger protein 4 (RNF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu11989	NM_001185010.3 Latest version!	Homo sapiens ring finger protein 4 (RNF4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu11989	NM_001185010.2	Homo sapiens ring finger protein 4 (RNF4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu10761	NM_002938.4	Homo sapiens ring finger protein 4 (RNF4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF4 ( NM_002938.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002929.1 Homo sapiens ring finger protein 4 (RNF4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10761
Clone ID Related Accession (Same CDS sequence)	NM_002938.5 , NM_001185009.2 , NM_002938.4
Accession Version	NM_002938.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA342512.1, AB000468.1, U95140.1 and BX322586.11. On Nov 23, 2018 this sequence version replaced NM_002938.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1057758.1, SRR3476690.759234.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000314289.13/ ENSP00000315212.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGAAAGAA GAAGACCAAG GAGGAATGCT 
AGGAGGCTGC CCCAGGACCA TGCTGACAGC TGTGTGGTGA GCAGTGACGA TGAGGAGTTG 
TCCAGGGACA GAGACGTATA TGTGACTACC CATACTCCCA GAAACGCCAG GGATGAGGGC 
GCTACAGGCC TCAGGCCCTC AGGTACTGTC AGTTGTCCCA TCTGCATGGA CGGATACTCA 
GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT GCGGCCATGT CTTCTGTAGC 
CAGTGCCTCC GTGATTCCCT GAAGAATGCT AATACTTGCC CAACTTGTAG GAAAAAGATC 
AACCACAAAC GGTACCACCC CATTTATATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002929.1
CDS	311..883
Translation	MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	NM_002938.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 4 (RNF4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002938.5

ORF Insert Sequence:

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGAAAGAA GAAGACCAAG GAGGAATGCT 
AGGAGGCTGC CCCAGGACCA TGCTGACAGC TGTGTGGTGA GCAGTGACGA TGAGGAGTTG 
TCCAGGGACA GAGACGTATA TGTGACTACC CATACTCCCA GAAACGCCAG GGATGAGGGC 
GCTACAGGCC TCAGGCCCTC AGGTACTGTC AGTTGTCCCA TCTGCATGGA CGGATACTCA 
GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT GCGGCCATGT CTTCTGTAGC 
CAGTGCCTCC GTGATTCCCT GAAGAATGCT AATACTTGCC CAACTTGTAG GAAAAAGATC 
AACCACAAAC GGTACCACCC CATTTATATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF4 ( NM_001185009.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171938.1 Homo sapiens ring finger protein 4 (RNF4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10761
Clone ID Related Accession (Same CDS sequence)	NM_002938.5 , NM_001185009.2 , NM_002938.4
Accession Version	NM_001185009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB064628.1, BC031935.1, AB000468.1, U95140.1, BX322586.11 and BU682428.1. On Jun 15, 2010 this sequence version replaced NM_001185009.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031935.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGAAAGAA GAAGACCAAG GAGGAATGCT 
AGGAGGCTGC CCCAGGACCA TGCTGACAGC TGTGTGGTGA GCAGTGACGA TGAGGAGTTG 
TCCAGGGACA GAGACGTATA TGTGACTACC CATACTCCCA GAAACGCCAG GGATGAGGGC 
GCTACAGGCC TCAGGCCCTC AGGTACTGTC AGTTGTCCCA TCTGCATGGA CGGATACTCA 
GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT GCGGCCATGT CTTCTGTAGC 
CAGTGCCTCC GTGATTCCCT GAAGAATGCT AATACTTGCC CAACTTGTAG GAAAAAGATC 
AACCACAAAC GGTACCACCC CATTTATATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171938.1
CDS	871..1443
Translation	MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	NM_001185009.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 4 (RNF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185009.2

ORF Insert Sequence:

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGAAAGAA GAAGACCAAG GAGGAATGCT 
AGGAGGCTGC CCCAGGACCA TGCTGACAGC TGTGTGGTGA GCAGTGACGA TGAGGAGTTG 
TCCAGGGACA GAGACGTATA TGTGACTACC CATACTCCCA GAAACGCCAG GGATGAGGGC 
GCTACAGGCC TCAGGCCCTC AGGTACTGTC AGTTGTCCCA TCTGCATGGA CGGATACTCA 
GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT GCGGCCATGT CTTCTGTAGC 
CAGTGCCTCC GTGATTCCCT GAAGAATGCT AATACTTGCC CAACTTGTAG GAAAAAGATC 
AACCACAAAC GGTACCACCC CATTTATATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF4 ( NM_002938.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002929.1 Homo sapiens ring finger protein 4 (RNF4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10761
Clone ID Related Accession (Same CDS sequence)	NM_002938.5 , NM_001185009.2 , NM_002938.4
Accession Version	NM_002938.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512403200000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA342512.1, AB000468.1, U95140.1, BX322586.11 and BU682428.1. On Jun 15, 2010 this sequence version replaced NM_002938.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.258155.1, SRR1803611.113715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGAAAGAA GAAGACCAAG GAGGAATGCT 
AGGAGGCTGC CCCAGGACCA TGCTGACAGC TGTGTGGTGA GCAGTGACGA TGAGGAGTTG 
TCCAGGGACA GAGACGTATA TGTGACTACC CATACTCCCA GAAACGCCAG GGATGAGGGC 
GCTACAGGCC TCAGGCCCTC AGGTACTGTC AGTTGTCCCA TCTGCATGGA CGGATACTCA 
GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT GCGGCCATGT CTTCTGTAGC 
CAGTGCCTCC GTGATTCCCT GAAGAATGCT AATACTTGCC CAACTTGTAG GAAAAAGATC 
AACCACAAAC GGTACCACCC CATTTATATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002929.1
CDS	349..921
Translation	MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	NM_002938.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 4 (RNF4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002938.4

ORF Insert Sequence:

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGAAAGAA GAAGACCAAG GAGGAATGCT 
AGGAGGCTGC CCCAGGACCA TGCTGACAGC TGTGTGGTGA GCAGTGACGA TGAGGAGTTG 
TCCAGGGACA GAGACGTATA TGTGACTACC CATACTCCCA GAAACGCCAG GGATGAGGGC 
GCTACAGGCC TCAGGCCCTC AGGTACTGTC AGTTGTCCCA TCTGCATGGA CGGATACTCA 
GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT GCGGCCATGT CTTCTGTAGC 
CAGTGCCTCC GTGATTCCCT GAAGAATGCT AATACTTGCC CAACTTGTAG GAAAAAGATC 
AACCACAAAC GGTACCACCC CATTTATATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF4 ( NM_001185010.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171939.1 Homo sapiens ring finger protein 4 (RNF4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11989
Clone ID Related Accession (Same CDS sequence)	NM_001185010.3 , NM_001185010.2
Accession Version	NM_001185010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA342512.1, AK309509.1, AB000468.1, U95140.1 and BX322586.11. On May 31, 2019 this sequence version replaced NM_001185010.2. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate exon in the 3' coding region which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.229889.1, SRR6380200.125803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGGCCCTC AGGTACTGTC AGTTGTCCCA 
TCTGCATGGA CGGATACTCA GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT 
GCGGCCATGT CTTCTGTAGC CAGTGCCTCC GTGATTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171939.1
CDS	311..652
Translation	MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDGPQVLSVVPSAWTDTQRSCRMDVSSFPQNAAMSSVASASVIP

Target ORF information:

RefSeq Version	NM_001185010.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 4 (RNF4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185010.3

ORF Insert Sequence:

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGGCCCTC AGGTACTGTC AGTTGTCCCA 
TCTGCATGGA CGGATACTCA GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT 
GCGGCCATGT CTTCTGTAGC CAGTGCCTCC GTGATTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF4 ( NM_001185010.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171939.1 Homo sapiens ring finger protein 4 (RNF4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11989
Clone ID Related Accession (Same CDS sequence)	NM_001185010.3 , NM_001185010.2
Accession Version	NM_001185010.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1544716800000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA342512.1, AK309509.1, AB000468.1, U95140.1, BX322586.11 and BU682428.1. On Jun 15, 2010 this sequence version replaced NM_001185010.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate exon in the 3' coding region which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.133534.1, SRR1803616.128987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGGCCCTC AGGTACTGTC AGTTGTCCCA 
TCTGCATGGA CGGATACTCA GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT 
GCGGCCATGT CTTCTGTAGC CAGTGCCTCC GTGATTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171939.1
CDS	349..690
Translation	MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDGPQVLSVVPSAWTDTQRSCRMDVSSFPQNAAMSSVASASVIP

Target ORF information:

RefSeq Version	NM_001185010.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 4 (RNF4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185010.2

ORF Insert Sequence:

ATGAGTACAA GAAAGCGTCG TGGTGGAGCA ATAAATTCTA GACAAGCTCA GAAGCGAACT 
CGGGAAGCAA CCTCCACCCC CGAGATCTCC TTGGAAGCAG AACCCATAGA ACTCGTGGAA 
ACTGCTGGAG ATGAAATTGT GGACCTCACT TGTGAATCTT TAGAGCCTGT GGTGGTTGAT 
CTGACTCACA ATGACTCTGT TGTGATTGTT GACGGCCCTC AGGTACTGTC AGTTGTCCCA 
TCTGCATGGA CGGATACTCA GAGATCGTGC AGAATGGACG TCTCATCGTT TCCACAGAAT 
GCGGCCATGT CTTCTGTAGC CAGTGCCTCC GTGATTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SUMOylation by SUMO2 is implicated in the degradation of misfolded ataxin-7 via RNF4 in SCA7 models.
Disease models & mechanisms12(1)(2019 01)
Marinello M,Werner A,Giannone M,Tahiri K,Alves S,Tesson C,den Dunnen W,Seeler JS,Brice A,Sittler A

RNF4 regulates zebrafish granulopoiesis through the DNMT1-C/EBPα axis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology32(9)4930-4940(2018 09)
Wang L,Liu X,Wang H,Yuan H,Chen S,Chen Z,de The H,Zhou J,Zhu J

E1B-55K-Mediated Regulation of RNF4 SUMO-Targeted Ubiquitin Ligase Promotes Human Adenovirus Gene Expression.
Journal of virology92(13)(2018 07)
Müncheberg S,Hay RT,Ip WH,Meyer T,Weiß C,Brenke J,Masser S,Hadian K,Dobner T,Schreiner S

Respiratory syncytial virus induces NRF2 degradation through a promyelocytic leukemia protein - ring finger protein 4 dependent pathway.
Free radical biology & medicine113494-504(2017 12)
Komaravelli N,Ansar M,Garofalo RP,Casola A

The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery.
Nature communications8(1)1809(2017 11)
Kumar R,González-Prieto R,Xiao Z,Verlaan-de Vries M,Vertegaal ACO

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RNF4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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