RHEB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHEB
Entrez Gene ID	6009
Full Name	Ras homolog, mTORC1 binding
Synonyms	RHEB2
General protein information	Preferred Names Ras homolog, mTORC1 binding Names GTP-binding protein Rheb Ras homolog enriched in brain 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q36.1
Summary	This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008].
Disorder MIM:	601293

mRNA and Protein(s)

mRNA	Protein	Name
XM_011516457.1	XP_011514759.1	GTP-binding protein Rheb isoform X1
XM_011516457.2	XP_011514759.1	GTP-binding protein Rheb isoform X1
NM_005614.3	NP_005605.1	GTP-binding protein Rheb
XM_024446854.1	XP_024302622.1	GTP-binding protein Rheb isoform X1
NM_005614.4	NP_005605.1	GTP-binding protein Rheb

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RHEB	XP_519513.1
Macaca mulatta (Rhesus monkey)	RHEB	XP_001104682.1
Canis lupus familiaris (dog)	RHEB	XP_532768.1
Schizosaccharomyces pombe (fission yeast)	rhb1	NP_595194.1
Magnaporthe oryzae (rice blast fungus)	MGG_06914	XP_003709648.1
Neurospora crassa	NCU01444	XP_955966.1
Homo sapiens (human)	RHEB	NP_005605.1
Bos taurus (cattle)	RHEB	NP_001026934.1
Mus musculus (house mouse)	Rheb	NP_444305.2
Caenorhabditis elegans (roundworm)	rheb-1	NP_499079.1
Saccharomyces cerevisiae (baker's yeast)	RHB1	NP_009956.2
Kluyveromyces lactis	KLLA0A11330g	XP_451492.1
Danio rerio (zebrafish)	zgc:73144	NP_957023.1
Xenopus tropicalis (tropical clawed frog)	rheb	NP_001015922.1
Rattus norvegicus (Norway rat)	Rheb	NP_037348.1
Gallus gallus (chicken)	RHEB	XP_001232988.3

Gene Ontology
Bibliography

Related articles in PubMed

[Polymorphisms of mTORC1 genes and risk of primary colorectal adenocarcinoma in Chinese populations].
Yu L, Liu ZY, Jiao J, Shi XL, Cui WL, Zhang W, Li QX
Zhonghua bing li xue za zhi = Chinese journal of pathology47(7)492-498(2018 Jul)

Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase.
Záhonová K, Petrželková R, Valach M, Yazaki E, Tikhonenkov DV, Butenko A, Janouškovec J, Hrdá Š, Klimeš V, Burger G, Inagaki Y, Keeling PJ, Hampl V, Flegontov P, Yurchenko V, Eliáš M
Scientific reports8(1)5239(2018 03)

NMR analysis of the backbone dynamics of the small GTPase Rheb and its interaction with the regulatory protein FKBP38.
De Cicco M, Kiss L, Dames SA
FEBS letters592(1)130-146(2018 01)

An oncogenic mutant of RHEB, RHEB Y35N, exhibits an altered interaction with BRAF resulting in cancer transformation.
Heard JJ, Phung I, Potes MI, Tamanoi F
BMC cancer18(1)69(2018 01)

Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40.
Yang H, Jiang X, Li B, Yang HJ, Miller M, Yang A, Dhar A, Pavletich NP
Nature552(7685)368-373(2017 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RHEB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHEB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu41704	XM_011516457.2 Latest version!	Homo sapiens Ras homolog, mTORC1 binding (RHEB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu41704	XM_024446854.1 Latest version!	Homo sapiens Ras homolog, mTORC1 binding (RHEB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu17242	NM_005614.4 Latest version!	Homo sapiens Ras homolog, mTORC1 binding (RHEB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$104.30 $149.00
View Old Accession Versions >>
OHu41704	XM_011516457.1	Homo sapiens Ras homolog enriched in brain (RHEB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu17242	NM_005614.3	Homo sapiens Ras homolog, mTORC1 binding (RHEB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHEB ( XM_011516457.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514759.1 Homo sapiens Ras homolog enriched in brain (RHEB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu41704
Clone ID Related Accession (Same CDS sequence)	XM_011516457.1 , XM_011516457.2 , XM_024446854.1
Accession Version	XM_011516457.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	GTP-binding protein Rheb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACAT TTAATTTAGG GAAATCCTCA TTGACGATTC AATTTGTTGA AGGCCAATTT 
GTGGACTCCT ACGATCCAAC CATAGAAAAC ACTTTTACAA AGTTGATCAC AGTAAATGGA 
CAAGAATATC ATCTTCAACT TGTAGACACA GCCGGGCAAG ATGAATATTC TATCTTTCCT 
CAGACATACT CCATAGATAT TAATGGCTAT ATTCTTGTGT ATTCTGTTAC ATCAATCAAA 
AGTTTTGAAG TGATTAAAGT TATCCATGGC AAATTGTTGG ATATGGTGGG GAAAGTACAA 
ATACCTATTA TGTTGGTTGG GAATAAGAAA GACCTGCATA TGGAAAGGGT GATCAGTTAT 
GAAGAAGGGA AAGCTTTGGC AGAATCTTGG AATGCAGCTT TTTTGGAATC TTCTGCTAAA 
GAAAATCAGA CTGCTGTGGA TGTTTTTCGA AGGATAATTT TGGAGGCAGA AAAAATGGAC 
GGGGCAGCTT CACAAGGCAA GTCTTCATGC TCGGTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514759.1
CDS	501..1022
Translation	MGTFNLGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Target ORF information:

RefSeq Version	XM_011516457.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras homolog enriched in brain (RHEB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516457.1

ORF Insert Sequence:

ATGGGCACAT TTAATTTAGG GAAATCCTCA TTGACGATTC AATTTGTTGA AGGCCAATTT 
GTGGACTCCT ACGATCCAAC CATAGAAAAC ACTTTTACAA AGTTGATCAC AGTAAATGGA 
CAAGAATATC ATCTTCAACT TGTAGACACA GCCGGGCAAG ATGAATATTC TATCTTTCCT 
CAGACATACT CCATAGATAT TAATGGCTAT ATTCTTGTGT ATTCTGTTAC ATCAATCAAA 
AGTTTTGAAG TGATTAAAGT TATCCATGGC AAATTGTTGG ATATGGTGGG GAAAGTACAA 
ATACCTATTA TGTTGGTTGG GAATAAGAAA GACCTGCATA TGGAAAGGGT GATCAGTTAT 
GAAGAAGGGA AAGCTTTGGC AGAATCTTGG AATGCAGCTT TTTTGGAATC TTCTGCTAAA 
GAAAATCAGA CTGCTGTGGA TGTTTTTCGA AGGATAATTT TGGAGGCAGA AAAAATGGAC 
GGGGCAGCTT CACAAGGCAA GTCTTCATGC TCGGTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHEB ( XM_011516457.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514759.1 Homo sapiens Ras homolog, mTORC1 binding (RHEB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu41704
Clone ID Related Accession (Same CDS sequence)	XM_011516457.1 , XM_011516457.2 , XM_024446854.1
Accession Version	XM_011516457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	GTP-binding protein Rheb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011516457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACAT TTAATTTAGG GAAATCCTCA TTGACGATTC AATTTGTTGA AGGCCAATTT 
GTGGACTCCT ACGATCCAAC CATAGAAAAC ACTTTTACAA AGTTGATCAC AGTAAATGGA 
CAAGAATATC ATCTTCAACT TGTAGACACA GCCGGGCAAG ATGAATATTC TATCTTTCCT 
CAGACATACT CCATAGATAT TAATGGCTAT ATTCTTGTGT ATTCTGTTAC ATCAATCAAA 
AGTTTTGAAG TGATTAAAGT TATCCATGGC AAATTGTTGG ATATGGTGGG GAAAGTACAA 
ATACCTATTA TGTTGGTTGG GAATAAGAAA GACCTGCATA TGGAAAGGGT GATCAGTTAT 
GAAGAAGGGA AAGCTTTGGC AGAATCTTGG AATGCAGCTT TTTTGGAATC TTCTGCTAAA 
GAAAATCAGA CTGCTGTGGA TGTTTTTCGA AGGATAATTT TGGAGGCAGA AAAAATGGAC 
GGGGCAGCTT CACAAGGCAA GTCTTCATGC TCGGTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514759.1
CDS	496..1017
Translation	MGTFNLGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Target ORF information:

RefSeq Version	XM_011516457.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras homolog, mTORC1 binding (RHEB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516457.2

ORF Insert Sequence:

ATGGGCACAT TTAATTTAGG GAAATCCTCA TTGACGATTC AATTTGTTGA AGGCCAATTT 
GTGGACTCCT ACGATCCAAC CATAGAAAAC ACTTTTACAA AGTTGATCAC AGTAAATGGA 
CAAGAATATC ATCTTCAACT TGTAGACACA GCCGGGCAAG ATGAATATTC TATCTTTCCT 
CAGACATACT CCATAGATAT TAATGGCTAT ATTCTTGTGT ATTCTGTTAC ATCAATCAAA 
AGTTTTGAAG TGATTAAAGT TATCCATGGC AAATTGTTGG ATATGGTGGG GAAAGTACAA 
ATACCTATTA TGTTGGTTGG GAATAAGAAA GACCTGCATA TGGAAAGGGT GATCAGTTAT 
GAAGAAGGGA AAGCTTTGGC AGAATCTTGG AATGCAGCTT TTTTGGAATC TTCTGCTAAA 
GAAAATCAGA CTGCTGTGGA TGTTTTTCGA AGGATAATTT TGGAGGCAGA AAAAATGGAC 
GGGGCAGCTT CACAAGGCAA GTCTTCATGC TCGGTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHEB ( XM_024446854.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302622.1 Homo sapiens Ras homolog, mTORC1 binding (RHEB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu41704
Clone ID Related Accession (Same CDS sequence)	XM_011516457.1 , XM_011516457.2 , XM_024446854.1
Accession Version	XM_024446854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	GTP-binding protein Rheb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACAT TTAATTTAGG GAAATCCTCA TTGACGATTC AATTTGTTGA AGGCCAATTT 
GTGGACTCCT ACGATCCAAC CATAGAAAAC ACTTTTACAA AGTTGATCAC AGTAAATGGA 
CAAGAATATC ATCTTCAACT TGTAGACACA GCCGGGCAAG ATGAATATTC TATCTTTCCT 
CAGACATACT CCATAGATAT TAATGGCTAT ATTCTTGTGT ATTCTGTTAC ATCAATCAAA 
AGTTTTGAAG TGATTAAAGT TATCCATGGC AAATTGTTGG ATATGGTGGG GAAAGTACAA 
ATACCTATTA TGTTGGTTGG GAATAAGAAA GACCTGCATA TGGAAAGGGT GATCAGTTAT 
GAAGAAGGGA AAGCTTTGGC AGAATCTTGG AATGCAGCTT TTTTGGAATC TTCTGCTAAA 
GAAAATCAGA CTGCTGTGGA TGTTTTTCGA AGGATAATTT TGGAGGCAGA AAAAATGGAC 
GGGGCAGCTT CACAAGGCAA GTCTTCATGC TCGGTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302622.1
CDS	620..1141
Translation	MGTFNLGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Target ORF information:

RefSeq Version	XM_024446854.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras homolog, mTORC1 binding (RHEB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446854.1

ORF Insert Sequence:

ATGGGCACAT TTAATTTAGG GAAATCCTCA TTGACGATTC AATTTGTTGA AGGCCAATTT 
GTGGACTCCT ACGATCCAAC CATAGAAAAC ACTTTTACAA AGTTGATCAC AGTAAATGGA 
CAAGAATATC ATCTTCAACT TGTAGACACA GCCGGGCAAG ATGAATATTC TATCTTTCCT 
CAGACATACT CCATAGATAT TAATGGCTAT ATTCTTGTGT ATTCTGTTAC ATCAATCAAA 
AGTTTTGAAG TGATTAAAGT TATCCATGGC AAATTGTTGG ATATGGTGGG GAAAGTACAA 
ATACCTATTA TGTTGGTTGG GAATAAGAAA GACCTGCATA TGGAAAGGGT GATCAGTTAT 
GAAGAAGGGA AAGCTTTGGC AGAATCTTGG AATGCAGCTT TTTTGGAATC TTCTGCTAAA 
GAAAATCAGA CTGCTGTGGA TGTTTTTCGA AGGATAATTT TGGAGGCAGA AAAAATGGAC 
GGGGCAGCTT CACAAGGCAA GTCTTCATGC TCGGTGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHEB ( NM_005614.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005605.1 Homo sapiens Ras homolog, mTORC1 binding (RHEB), mRNA.

Price & Availability↑

CloneID	OHu17242
Clone ID Related Accession (Same CDS sequence)	NM_005614.3 , NM_005614.4
Accession Version	NM_005614.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514649600000
Organism	Homo sapiens(human)
Product	GTP-binding protein Rheb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG702213.1, BC016155.1, Z29677.1, DA475169.1 and BM670621.1. On May 25, 2006 this sequence version replaced NM_005614.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK125446.1, SRR1803616.272360.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCAGT CCAAGTCCCG GAAGATCGCG ATCCTGGGCT ACCGGTCTGT GGGGAAATCC 
TCATTGACGA TTCAATTTGT TGAAGGCCAA TTTGTGGACT CCTACGATCC AACCATAGAA 
AACACTTTTA CAAAGTTGAT CACAGTAAAT GGACAAGAAT ATCATCTTCA ACTTGTAGAC 
ACAGCCGGGC AAGATGAATA TTCTATCTTT CCTCAGACAT ACTCCATAGA TATTAATGGC 
TATATTCTTG TGTATTCTGT TACATCAATC AAAAGTTTTG AAGTGATTAA AGTTATCCAT 
GGCAAATTGT TGGATATGGT GGGGAAAGTA CAAATACCTA TTATGTTGGT TGGGAATAAG 
AAAGACCTGC ATATGGAAAG GGTGATCAGT TATGAAGAAG GGAAAGCTTT GGCAGAATCT 
TGGAATGCAG CTTTTTTGGA ATCTTCTGCT AAAGAAAATC AGACTGCTGT GGATGTTTTT 
CGAAGGATAA TTTTGGAGGC AGAAAAAATG GACGGGGCAG CTTCACAAGG CAAGTCTTCA 
TGCTCGGTGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005605.1
CDS	414..968
Translation	MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Target ORF information:

RefSeq Version	NM_005614.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras homolog, mTORC1 binding (RHEB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005614.3

ORF Insert Sequence:

ATGCCGCAGT CCAAGTCCCG GAAGATCGCG ATCCTGGGCT ACCGGTCTGT GGGGAAATCC 
TCATTGACGA TTCAATTTGT TGAAGGCCAA TTTGTGGACT CCTACGATCC AACCATAGAA 
AACACTTTTA CAAAGTTGAT CACAGTAAAT GGACAAGAAT ATCATCTTCA ACTTGTAGAC 
ACAGCCGGGC AAGATGAATA TTCTATCTTT CCTCAGACAT ACTCCATAGA TATTAATGGC 
TATATTCTTG TGTATTCTGT TACATCAATC AAAAGTTTTG AAGTGATTAA AGTTATCCAT 
GGCAAATTGT TGGATATGGT GGGGAAAGTA CAAATACCTA TTATGTTGGT TGGGAATAAG 
AAAGACCTGC ATATGGAAAG GGTGATCAGT TATGAAGAAG GGAAAGCTTT GGCAGAATCT 
TGGAATGCAG CTTTTTTGGA ATCTTCTGCT AAAGAAAATC AGACTGCTGT GGATGTTTTT 
CGAAGGATAA TTTTGGAGGC AGAAAAAATG GACGGGGCAG CTTCACAAGG CAAGTCTTCA 
TGCTCGGTGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHEB ( NM_005614.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005605.1 Homo sapiens Ras homolog, mTORC1 binding (RHEB), mRNA.

Price & Availability↑

CloneID	OHu17242
Clone ID Related Accession (Same CDS sequence)	NM_005614.3 , NM_005614.4
Accession Version	NM_005614.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	GTP-binding protein Rheb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG702213.1, BC016155.1, Z29677.1, DA475169.1 and BM670621.1. On Nov 22, 2018 this sequence version replaced NM_005614.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.610406.1, AK125446.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262187.10/ ENSP00000262187.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGCAGT CCAAGTCCCG GAAGATCGCG ATCCTGGGCT ACCGGTCTGT GGGGAAATCC 
TCATTGACGA TTCAATTTGT TGAAGGCCAA TTTGTGGACT CCTACGATCC AACCATAGAA 
AACACTTTTA CAAAGTTGAT CACAGTAAAT GGACAAGAAT ATCATCTTCA ACTTGTAGAC 
ACAGCCGGGC AAGATGAATA TTCTATCTTT CCTCAGACAT ACTCCATAGA TATTAATGGC 
TATATTCTTG TGTATTCTGT TACATCAATC AAAAGTTTTG AAGTGATTAA AGTTATCCAT 
GGCAAATTGT TGGATATGGT GGGGAAAGTA CAAATACCTA TTATGTTGGT TGGGAATAAG 
AAAGACCTGC ATATGGAAAG GGTGATCAGT TATGAAGAAG GGAAAGCTTT GGCAGAATCT 
TGGAATGCAG CTTTTTTGGA ATCTTCTGCT AAAGAAAATC AGACTGCTGT GGATGTTTTT 
CGAAGGATAA TTTTGGAGGC AGAAAAAATG GACGGGGCAG CTTCACAAGG CAAGTCTTCA 
TGCTCGGTGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005605.1
CDS	385..939
Translation	MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Target ORF information:

RefSeq Version	NM_005614.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras homolog, mTORC1 binding (RHEB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005614.4

ORF Insert Sequence:

ATGCCGCAGT CCAAGTCCCG GAAGATCGCG ATCCTGGGCT ACCGGTCTGT GGGGAAATCC 
TCATTGACGA TTCAATTTGT TGAAGGCCAA TTTGTGGACT CCTACGATCC AACCATAGAA 
AACACTTTTA CAAAGTTGAT CACAGTAAAT GGACAAGAAT ATCATCTTCA ACTTGTAGAC 
ACAGCCGGGC AAGATGAATA TTCTATCTTT CCTCAGACAT ACTCCATAGA TATTAATGGC 
TATATTCTTG TGTATTCTGT TACATCAATC AAAAGTTTTG AAGTGATTAA AGTTATCCAT 
GGCAAATTGT TGGATATGGT GGGGAAAGTA CAAATACCTA TTATGTTGGT TGGGAATAAG 
AAAGACCTGC ATATGGAAAG GGTGATCAGT TATGAAGAAG GGAAAGCTTT GGCAGAATCT 
TGGAATGCAG CTTTTTTGGA ATCTTCTGCT AAAGAAAATC AGACTGCTGT GGATGTTTTT 
CGAAGGATAA TTTTGGAGGC AGAAAAAATG GACGGGGCAG CTTCACAAGG CAAGTCTTCA 
TGCTCGGTGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Polymorphisms of mTORC1 genes and risk of primary colorectal adenocarcinoma in Chinese populations].
Zhonghua bing li xue za zhi = Chinese journal of pathology47(7)492-498(2018 Jul)
Yu L,Liu ZY,Jiao J,Shi XL,Cui WL,Zhang W,Li QX

Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase.
Scientific reports8(1)5239(2018 03)
Záhonová K,Petrželková R,Valach M,Yazaki E,Tikhonenkov DV,Butenko A,Janouškovec J,Hrdá Š,Klimeš V,Burger G,Inagaki Y,Keeling PJ,Hampl V,Flegontov P,Yurchenko V,Eliáš M

NMR analysis of the backbone dynamics of the small GTPase Rheb and its interaction with the regulatory protein FKBP38.
FEBS letters592(1)130-146(2018 01)
De Cicco M,Kiss L,Dames SA

An oncogenic mutant of RHEB, RHEB Y35N, exhibits an altered interaction with BRAF resulting in cancer transformation.
BMC cancer18(1)69(2018 01)
Heard JJ,Phung I,Potes MI,Tamanoi F

Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40.
Nature552(7685)368-373(2017 12)
Yang H,Jiang X,Li B,Yang HJ,Miller M,Yang A,Dhar A,Pavletich NP

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RHEB cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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