QARS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	QARS
Entrez Gene ID	5859
Full Name	glutaminyl-tRNA synthetase
Synonyms	GLNRS,MSCCA,PRO2195
General protein information	Preferred Names glutaminyl-tRNA synthetase Names glutamine--tRNA ligase glutamine-tRNA synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013].
Disorder MIM:	603727
Disorder Html:	Microcephaly, progressive, seizures, and cerebral and cerebellar atrophy, 615760 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017006965.1	XP_016862454.1	glutamine--tRNA ligase isoform X1
XM_017006965.2	XP_016862454.2	glutamine--tRNA ligase isoform X1
NM_005051.2	NP_005042.1	glutamine--tRNA ligase isoform a
NM_001272073.2	NP_001259002.1	glutamine--tRNA ligase isoform b
NM_005051.3	NP_005042.1	glutamine--tRNA ligase isoform a
NM_001272073.1	NP_001259002.1	glutamine--tRNA ligase isoform b

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR259C	NP_986925.1
Canis lupus familiaris (dog)	QARS	XP_005632622.1
Bos taurus (cattle)	QARS	NP_001029640.1
Magnaporthe oryzae (rice blast fungus)	MGG_09393	XP_003720218.1
Oryza sativa (rice)	Os01g0185200	NP_001042238.1
Gallus gallus (chicken)	QARS	NP_001012800.1
Saccharomyces cerevisiae (baker's yeast)	GLN4	NP_014811.3
Xenopus tropicalis (tropical clawed frog)	qars	XP_004914154.1
Danio rerio (zebrafish)	qars	NP_957507.1
Caenorhabditis elegans (roundworm)	qars-1	NP_001255843.1
Schizosaccharomyces pombe (fission yeast)	qrs1	NP_596745.1
Kluyveromyces lactis	KLLA0F20449g	XP_455998.1
Homo sapiens (human)	QARS	NP_005042.1
Pan troglodytes (chimpanzee)	QARS	XP_001147632.3
Drosophila melanogaster (fruit fly)	Aats-gln	NP_524841.1
Oryza sativa (rice)	Os05g0182800	NP_001054822.1
Macaca mulatta (Rhesus monkey)	QARS	XP_001110256.2
Arabidopsis thaliana (thale cress)	OVA9	NP_173906.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010267	XP_319458.3
Neurospora crassa	NCU07926	XP_962442.1
Rattus norvegicus (Norway rat)	LOC100911132	XP_003752277.1

Gene Ontology
Bibliography

Related articles in PubMed

Severe growth deficiency, microcephaly, intellectual disability, and characteristic facial features are due to a homozygous QARS mutation.
Leshinsky-Silver E, Ling J, Wu J, Vinkler C, Yosovich K, Bahar S, Yanoov-Sharav M, Lerman-Sagie T, Lev D
Neurogenetics18(3)141-146(2017 Jul)

Human cytomegalovirus-encoded miR-US4-1 promotes cell apoptosis and benefits discharge of infectious virus particles by targeting QARS.
Shao Y, Qi Y, Huang Y, Liu Z, Ma Y, Guo X, Jiang S, Sun Z, Ruan Q
Journal of biosciences41(2)183-92(2016 Jun)

The crystal structure of human GlnRS provides basis for the development of neurological disorders.
Ognjenović J, Wu J, Matthies D, Baxa U, Subramaniam S, Ling J, Simonović M
Nucleic acids research44(7)3420-31(2016 Apr)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kovács IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barabási AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Mutations in the glutaminyl-tRNA synthetase gene cause early-onset epileptic encephalopathy.
Kodera H, Osaka H, Iai M, Aida N, Yamashita A, Tsurusaki Y, Nakashima M, Miyake N, Saitsu H, Matsumoto N
Journal of human genetics60(2)97-101(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following QARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the QARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17748	NM_001272073.2 Latest version!	Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu105418	XM_017006965.2 Latest version!	Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94441	XM_017006965.1 Latest version!	Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu17745	NM_005051.3 Latest version!	Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$545.30 $779.00
View Old Accession Versions >>
OHu17745	NM_005051.2	Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$545.30 $779.00
OHu17748	NM_001272073.1	Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

QARS ( NM_001272073.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001259002.1 Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant 2, mRNA.

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CloneID	OHu17748
Clone ID Related Accession (Same CDS sequence)	NM_001272073.2 , NM_001272073.1
Accession Version	NM_001272073.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Homo sapiens(human)
Product	glutamine--tRNA ligase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK301559.1 and BC000394.2. On May 31, 2019 this sequence version replaced NM_001272073.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301559.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:22223895) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CACTGCCCTT 
GAGTATGTGC GGAGTCACCC CTTGGACCCC ATCGACACTG TGGACTTCGA GCGGGAATGT 
GGCGTGGGTG TCATTGTGAC CCCAGAGCAG ATTGAGGAGG CTGTGGAGGC TGCTATTAAC 
AGGCACCGGC CCCAGCTCCT GGTGGAACGT TACCATTTCA ACATGGGGCT GCTGATGGGA 
GAGGCTCGGG CTGTGCTGAA GTGGGCAGAT GGCAAAATGA TCAAGAATGA AGTGGACATG 
CAGGTCCTCC ACCTTCTGGG CCCCAAGTTG GAGGCTGATC TGGAGAAGAA GTTCAAGGTG 
GCAAAAGCTC GGCTAGAAGA AACAGACCGG AGGACGGCAA AGGATGTGGT GGAGAATGGC 
GAGACTGCTG ACCAGACCCT GTCTCTGATG GAGCAGCTCC GGGGGGAGGC CCTTAAGTTC 
CACAAGCCTG GTGAGAACTA CAAGACCCCA GGCTATGTGG TCACTCCACA CACCATGAAT 
CTACTAAAGC AGCACCTGGA GATTACTGGT GGGCAGGTAC GTACCCGGTT CCCGCCAGAA 
CCCAATGGAA TCCTGCATAT TGGACATGCC AAAGCCATCA ATTTCAACTT TGGCTATGCC 
AAGGCCAACA ATGGCATCTG TTTTCTGCGT TTTGATGACA CCAACCCTGA GAAGGAGGAA 
GCAAAGTTCT TCACGGCCAT CTGTGACATG GTAGCCTGGC TAGGCTACAC ACCTTACAAA 
GTCACATATG CGTCTGACTA TTTTGACCAG CTATATGCGT GGGCTGTGGA GCTCATCCGC 
AGGGGTCTGG CTTATGTGTG CCACCAGCGA GGAGAGGAGC TCAAAGGCCA TAATACTCTG 
CCTTCACCCT GGAGAGACCG TCCCATGGAG GAGTCACTGC TGCTCTTTGA GGCAATGCGC 
AAGGGCAAGT TTTCAGAGGG CGAGGCCACA CTACGGATGA AGCTGGTGAT GGAGGATGGC 
AAGATGGACC CTGTAGCCTA TCGAGTCAAG TATACACCAC ACCACCGCAC AGGGGACAAA 
TGGTGCATCT ATCCCACCTA CGACTACACA CACTGCCTCT GTGACTCCAT CGAGCACATC 
ACTCACTCAC TCTGCACCAA GGAATTCCAG GCCCGACGCT CTTCCTACTT CTGGCTTTGC 
AATGCACTGG ACGTCTATTG CCCTGTGCAG TGGGAGTATG GCCGCCTCAA CCTGCACTAT 
GCTGTTGTCT CTAAGAGGAA GATCCTCCAG CTTGTAGCAA CTGGTGCTGT GCGGGACTGG 
GATGACCCAC GGCTCTTTAC ACTCACGGCC CTGCGACGGC GGGGCTTCCC ACCTGAGGCC 
ATCAACAACT TCTGTGCCCG GGTGGGAGTG ACTGTGGCAC AAACCACAAT GGAGCCACAT 
CTTCTAGAAG CCTGTGTGCG TGATGTGCTG AATGACACAG CCCCACGAGC CATGGCTGTG 
CTGGAGTCAC TACGGGTCAT CATCACCAAC TTTCCTGCTG CCAAGTCCTT GGACATCCAG 
GTGCCCAACT TCCCAGCTGA TGAGACCAAA GGCTTCCATC AGGTTCCCTT TGCACCCATT 
GTCTTCATTG AGAGGACTGA CTTCAAGGAG GAGCCAGAGC CAGGATTTAA GCGCCTGGCT 
TGGGGCCAGC CTGTGGGCCT GAGGCATACA GGCTACGTCA TTGAGCTGCA GCATGTTGTC 
AAGGGCCCCA GTGGTTGTGT AGAGAGTCTG GAGGTGACCT GCAGACGGGC AGATGCTGGA 
GAGAAGCCAA AGGCCTTTAT TCACTGGGTG TCACAGCCTT TGATGTGTGA GGTTCGCCTC 
TATGAGCGAC TATTCCAGCA CAAGAACCCT GAAGATCCTA CTGAGGTGCC TGGTGGATTT 
TTAAGTGACC TGAACCTGGC ATCACTACAC GTGGTGGATG CAGCATTAGT GGACTGCTCT 
GTGGCCCTGG CAAAACCCTT CGACAAGTTC CAGTTTGAGC GTCTTGGATA TTTCTCCGTG 
GATCCAGACA GCCATCAGGG AAAGCTTGTC TTTAACCGAA CTGTCACACT GAAGGAAGAC 
CCAGGAAAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259002.1
CDS	25..2319
Translation	MAALDSLSLFTSLGLSEQKARETLKNSALSAQLREAATQAQQTLGSTIDKATGILLYGLASRLRDTRRLSFLVSYIATALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEVDMQVLHLLGPKLEADLEKKFKVAKARLEETDRRTAKDVVENGETADQTLSLMEQLRGEALKFHKPGENYKTPGYVVTPHTMNLLKQHLEITGGQVRTRFPPEPNGILHIGHAKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAICDMVAWLGYTPYKVTYASDYFDQLYAWAVELIRRGLAYVCHQRGEELKGHNTLPSPWRDRPMEESLLLFEAMRKGKFSEGEATLRMKLVMEDGKMDPVAYRVKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

Target ORF information:

RefSeq Version	NM_001272073.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272073.2

ORF Insert Sequence:

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CACTGCCCTT 
GAGTATGTGC GGAGTCACCC CTTGGACCCC ATCGACACTG TGGACTTCGA GCGGGAATGT 
GGCGTGGGTG TCATTGTGAC CCCAGAGCAG ATTGAGGAGG CTGTGGAGGC TGCTATTAAC 
AGGCACCGGC CCCAGCTCCT GGTGGAACGT TACCATTTCA ACATGGGGCT GCTGATGGGA 
GAGGCTCGGG CTGTGCTGAA GTGGGCAGAT GGCAAAATGA TCAAGAATGA AGTGGACATG 
CAGGTCCTCC ACCTTCTGGG CCCCAAGTTG GAGGCTGATC TGGAGAAGAA GTTCAAGGTG 
GCAAAAGCTC GGCTAGAAGA AACAGACCGG AGGACGGCAA AGGATGTGGT GGAGAATGGC 
GAGACTGCTG ACCAGACCCT GTCTCTGATG GAGCAGCTCC GGGGGGAGGC CCTTAAGTTC 
CACAAGCCTG GTGAGAACTA CAAGACCCCA GGCTATGTGG TCACTCCACA CACCATGAAT 
CTACTAAAGC AGCACCTGGA GATTACTGGT GGGCAGGTAC GTACCCGGTT CCCGCCAGAA 
CCCAATGGAA TCCTGCATAT TGGACATGCC AAAGCCATCA ATTTCAACTT TGGCTATGCC 
AAGGCCAACA ATGGCATCTG TTTTCTGCGT TTTGATGACA CCAACCCTGA GAAGGAGGAA 
GCAAAGTTCT TCACGGCCAT CTGTGACATG GTAGCCTGGC TAGGCTACAC ACCTTACAAA 
GTCACATATG CGTCTGACTA TTTTGACCAG CTATATGCGT GGGCTGTGGA GCTCATCCGC 
AGGGGTCTGG CTTATGTGTG CCACCAGCGA GGAGAGGAGC TCAAAGGCCA TAATACTCTG 
CCTTCACCCT GGAGAGACCG TCCCATGGAG GAGTCACTGC TGCTCTTTGA GGCAATGCGC 
AAGGGCAAGT TTTCAGAGGG CGAGGCCACA CTACGGATGA AGCTGGTGAT GGAGGATGGC 
AAGATGGACC CTGTAGCCTA TCGAGTCAAG TATACACCAC ACCACCGCAC AGGGGACAAA 
TGGTGCATCT ATCCCACCTA CGACTACACA CACTGCCTCT GTGACTCCAT CGAGCACATC 
ACTCACTCAC TCTGCACCAA GGAATTCCAG GCCCGACGCT CTTCCTACTT CTGGCTTTGC 
AATGCACTGG ACGTCTATTG CCCTGTGCAG TGGGAGTATG GCCGCCTCAA CCTGCACTAT 
GCTGTTGTCT CTAAGAGGAA GATCCTCCAG CTTGTAGCAA CTGGTGCTGT GCGGGACTGG 
GATGACCCAC GGCTCTTTAC ACTCACGGCC CTGCGACGGC GGGGCTTCCC ACCTGAGGCC 
ATCAACAACT TCTGTGCCCG GGTGGGAGTG ACTGTGGCAC AAACCACAAT GGAGCCACAT 
CTTCTAGAAG CCTGTGTGCG TGATGTGCTG AATGACACAG CCCCACGAGC CATGGCTGTG 
CTGGAGTCAC TACGGGTCAT CATCACCAAC TTTCCTGCTG CCAAGTCCTT GGACATCCAG 
GTGCCCAACT TCCCAGCTGA TGAGACCAAA GGCTTCCATC AGGTTCCCTT TGCACCCATT 
GTCTTCATTG AGAGGACTGA CTTCAAGGAG GAGCCAGAGC CAGGATTTAA GCGCCTGGCT 
TGGGGCCAGC CTGTGGGCCT GAGGCATACA GGCTACGTCA TTGAGCTGCA GCATGTTGTC 
AAGGGCCCCA GTGGTTGTGT AGAGAGTCTG GAGGTGACCT GCAGACGGGC AGATGCTGGA 
GAGAAGCCAA AGGCCTTTAT TCACTGGGTG TCACAGCCTT TGATGTGTGA GGTTCGCCTC 
TATGAGCGAC TATTCCAGCA CAAGAACCCT GAAGATCCTA CTGAGGTGCC TGGTGGATTT 
TTAAGTGACC TGAACCTGGC ATCACTACAC GTGGTGGATG CAGCATTAGT GGACTGCTCT 
GTGGCCCTGG CAAAACCCTT CGACAAGTTC CAGTTTGAGC GTCTTGGATA TTTCTCCGTG 
GATCCAGACA GCCATCAGGG AAAGCTTGTC TTTAACCGAA CTGTCACACT GAAGGAAGAC 
CCAGGAAAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

QARS ( NM_001272073.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001259002.1 Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17748
Clone ID Related Accession (Same CDS sequence)	NM_001272073.2 , NM_001272073.1
Accession Version	NM_001272073.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	glutamine--tRNA ligase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM560535.1, AK302867.1, DC403423.1, AK301559.1 and BC000394.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301559.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:22223895) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CACTGCCCTT 
GAGTATGTGC GGAGTCACCC CTTGGACCCC ATCGACACTG TGGACTTCGA GCGGGAATGT 
GGCGTGGGTG TCATTGTGAC CCCAGAGCAG ATTGAGGAGG CTGTGGAGGC TGCTATTAAC 
AGGCACCGGC CCCAGCTCCT GGTGGAACGT TACCATTTCA ACATGGGGCT GCTGATGGGA 
GAGGCTCGGG CTGTGCTGAA GTGGGCAGAT GGCAAAATGA TCAAGAATGA AGTGGACATG 
CAGGTCCTCC ACCTTCTGGG CCCCAAGTTG GAGGCTGATC TGGAGAAGAA GTTCAAGGTG 
GCAAAAGCTC GGCTAGAAGA AACAGACCGG AGGACGGCAA AGGATGTGGT GGAGAATGGC 
GAGACTGCTG ACCAGACCCT GTCTCTGATG GAGCAGCTCC GGGGGGAGGC CCTTAAGTTC 
CACAAGCCTG GTGAGAACTA CAAGACCCCA GGCTATGTGG TCACTCCACA CACCATGAAT 
CTACTAAAGC AGCACCTGGA GATTACTGGT GGGCAGGTAC GTACCCGGTT CCCGCCAGAA 
CCCAATGGAA TCCTGCATAT TGGACATGCC AAAGCCATCA ATTTCAACTT TGGCTATGCC 
AAGGCCAACA ATGGCATCTG TTTTCTGCGT TTTGATGACA CCAACCCTGA GAAGGAGGAA 
GCAAAGTTCT TCACGGCCAT CTGTGACATG GTAGCCTGGC TAGGCTACAC ACCTTACAAA 
GTCACATATG CGTCTGACTA TTTTGACCAG CTATATGCGT GGGCTGTGGA GCTCATCCGC 
AGGGGTCTGG CTTATGTGTG CCACCAGCGA GGAGAGGAGC TCAAAGGCCA TAATACTCTG 
CCTTCACCCT GGAGAGACCG TCCCATGGAG GAGTCACTGC TGCTCTTTGA GGCAATGCGC 
AAGGGCAAGT TTTCAGAGGG CGAGGCCACA CTACGGATGA AGCTGGTGAT GGAGGATGGC 
AAGATGGACC CTGTAGCCTA TCGAGTCAAG TATACACCAC ACCACCGCAC AGGGGACAAA 
TGGTGCATCT ATCCCACCTA CGACTACACA CACTGCCTCT GTGACTCCAT CGAGCACATC 
ACTCACTCAC TCTGCACCAA GGAATTCCAG GCCCGACGCT CTTCCTACTT CTGGCTTTGC 
AATGCACTGG ACGTCTATTG CCCTGTGCAG TGGGAGTATG GCCGCCTCAA CCTGCACTAT 
GCTGTTGTCT CTAAGAGGAA GATCCTCCAG CTTGTAGCAA CTGGTGCTGT GCGGGACTGG 
GATGACCCAC GGCTCTTTAC ACTCACGGCC CTGCGACGGC GGGGCTTCCC ACCTGAGGCC 
ATCAACAACT TCTGTGCCCG GGTGGGAGTG ACTGTGGCAC AAACCACAAT GGAGCCACAT 
CTTCTAGAAG CCTGTGTGCG TGATGTGCTG AATGACACAG CCCCACGAGC CATGGCTGTG 
CTGGAGTCAC TACGGGTCAT CATCACCAAC TTTCCTGCTG CCAAGTCCTT GGACATCCAG 
GTGCCCAACT TCCCAGCTGA TGAGACCAAA GGCTTCCATC AGGTTCCCTT TGCACCCATT 
GTCTTCATTG AGAGGACTGA CTTCAAGGAG GAGCCAGAGC CAGGATTTAA GCGCCTGGCT 
TGGGGCCAGC CTGTGGGCCT GAGGCATACA GGCTACGTCA TTGAGCTGCA GCATGTTGTC 
AAGGGCCCCA GTGGTTGTGT AGAGAGTCTG GAGGTGACCT GCAGACGGGC AGATGCTGGA 
GAGAAGCCAA AGGCCTTTAT TCACTGGGTG TCACAGCCTT TGATGTGTGA GGTTCGCCTC 
TATGAGCGAC TATTCCAGCA CAAGAACCCT GAAGATCCTA CTGAGGTGCC TGGTGGATTT 
TTAAGTGACC TGAACCTGGC ATCACTACAC GTGGTGGATG CAGCATTAGT GGACTGCTCT 
GTGGCCCTGG CAAAACCCTT CGACAAGTTC CAGTTTGAGC GTCTTGGATA TTTCTCCGTG 
GATCCAGACA GCCATCAGGG AAAGCTTGTC TTTAACCGAA CTGTCACACT GAAGGAAGAC 
CCAGGAAAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259002.1
CDS	397..2691
Translation	MAALDSLSLFTSLGLSEQKARETLKNSALSAQLREAATQAQQTLGSTIDKATGILLYGLASRLRDTRRLSFLVSYIATALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEVDMQVLHLLGPKLEADLEKKFKVAKARLEETDRRTAKDVVENGETADQTLSLMEQLRGEALKFHKPGENYKTPGYVVTPHTMNLLKQHLEITGGQVRTRFPPEPNGILHIGHAKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAICDMVAWLGYTPYKVTYASDYFDQLYAWAVELIRRGLAYVCHQRGEELKGHNTLPSPWRDRPMEESLLLFEAMRKGKFSEGEATLRMKLVMEDGKMDPVAYRVKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

Target ORF information:

RefSeq Version	NM_001272073.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272073.1

ORF Insert Sequence:

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CACTGCCCTT 
GAGTATGTGC GGAGTCACCC CTTGGACCCC ATCGACACTG TGGACTTCGA GCGGGAATGT 
GGCGTGGGTG TCATTGTGAC CCCAGAGCAG ATTGAGGAGG CTGTGGAGGC TGCTATTAAC 
AGGCACCGGC CCCAGCTCCT GGTGGAACGT TACCATTTCA ACATGGGGCT GCTGATGGGA 
GAGGCTCGGG CTGTGCTGAA GTGGGCAGAT GGCAAAATGA TCAAGAATGA AGTGGACATG 
CAGGTCCTCC ACCTTCTGGG CCCCAAGTTG GAGGCTGATC TGGAGAAGAA GTTCAAGGTG 
GCAAAAGCTC GGCTAGAAGA AACAGACCGG AGGACGGCAA AGGATGTGGT GGAGAATGGC 
GAGACTGCTG ACCAGACCCT GTCTCTGATG GAGCAGCTCC GGGGGGAGGC CCTTAAGTTC 
CACAAGCCTG GTGAGAACTA CAAGACCCCA GGCTATGTGG TCACTCCACA CACCATGAAT 
CTACTAAAGC AGCACCTGGA GATTACTGGT GGGCAGGTAC GTACCCGGTT CCCGCCAGAA 
CCCAATGGAA TCCTGCATAT TGGACATGCC AAAGCCATCA ATTTCAACTT TGGCTATGCC 
AAGGCCAACA ATGGCATCTG TTTTCTGCGT TTTGATGACA CCAACCCTGA GAAGGAGGAA 
GCAAAGTTCT TCACGGCCAT CTGTGACATG GTAGCCTGGC TAGGCTACAC ACCTTACAAA 
GTCACATATG CGTCTGACTA TTTTGACCAG CTATATGCGT GGGCTGTGGA GCTCATCCGC 
AGGGGTCTGG CTTATGTGTG CCACCAGCGA GGAGAGGAGC TCAAAGGCCA TAATACTCTG 
CCTTCACCCT GGAGAGACCG TCCCATGGAG GAGTCACTGC TGCTCTTTGA GGCAATGCGC 
AAGGGCAAGT TTTCAGAGGG CGAGGCCACA CTACGGATGA AGCTGGTGAT GGAGGATGGC 
AAGATGGACC CTGTAGCCTA TCGAGTCAAG TATACACCAC ACCACCGCAC AGGGGACAAA 
TGGTGCATCT ATCCCACCTA CGACTACACA CACTGCCTCT GTGACTCCAT CGAGCACATC 
ACTCACTCAC TCTGCACCAA GGAATTCCAG GCCCGACGCT CTTCCTACTT CTGGCTTTGC 
AATGCACTGG ACGTCTATTG CCCTGTGCAG TGGGAGTATG GCCGCCTCAA CCTGCACTAT 
GCTGTTGTCT CTAAGAGGAA GATCCTCCAG CTTGTAGCAA CTGGTGCTGT GCGGGACTGG 
GATGACCCAC GGCTCTTTAC ACTCACGGCC CTGCGACGGC GGGGCTTCCC ACCTGAGGCC 
ATCAACAACT TCTGTGCCCG GGTGGGAGTG ACTGTGGCAC AAACCACAAT GGAGCCACAT 
CTTCTAGAAG CCTGTGTGCG TGATGTGCTG AATGACACAG CCCCACGAGC CATGGCTGTG 
CTGGAGTCAC TACGGGTCAT CATCACCAAC TTTCCTGCTG CCAAGTCCTT GGACATCCAG 
GTGCCCAACT TCCCAGCTGA TGAGACCAAA GGCTTCCATC AGGTTCCCTT TGCACCCATT 
GTCTTCATTG AGAGGACTGA CTTCAAGGAG GAGCCAGAGC CAGGATTTAA GCGCCTGGCT 
TGGGGCCAGC CTGTGGGCCT GAGGCATACA GGCTACGTCA TTGAGCTGCA GCATGTTGTC 
AAGGGCCCCA GTGGTTGTGT AGAGAGTCTG GAGGTGACCT GCAGACGGGC AGATGCTGGA 
GAGAAGCCAA AGGCCTTTAT TCACTGGGTG TCACAGCCTT TGATGTGTGA GGTTCGCCTC 
TATGAGCGAC TATTCCAGCA CAAGAACCCT GAAGATCCTA CTGAGGTGCC TGGTGGATTT 
TTAAGTGACC TGAACCTGGC ATCACTACAC GTGGTGGATG CAGCATTAGT GGACTGCTCT 
GTGGCCCTGG CAAAACCCTT CGACAAGTTC CAGTTTGAGC GTCTTGGATA TTTCTCCGTG 
GATCCAGACA GCCATCAGGG AAAGCTTGTC TTTAACCGAA CTGTCACACT GAAGGAAGAC 
CCAGGAAAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

QARS ( XM_017006965.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862454.2 Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant X1, mRNA.

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CloneID	OHu105418
Clone ID Related Accession (Same CDS sequence)	XM_017006965.2
Accession Version	XM_017006965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	glutamine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CAGTAAGAAG 
ATCCACACTG AGCCCCAGCT AAGCGCTGCC CTTGAGTATG TGCGGAGTCA CCCCTTGGAC 
CCCATCGACA CTGTGGACTT CGAGCGGGAA TGTGGCGTGG GTGTCATTGT GACCCCAGAG 
CAGATTGAGG AGGCTGTGGA GGCTGCTATT AACAGGCACC GGCCCCAGCT CCTGGTGGAA 
CGTTACCATT TCAACATGGG GCTGCTGATG GGAGAGGCTC GGGCTGTGCT GAAGTGGGCA 
GATGGCAAAA TGATCAAGAA TGAAGTGGAC ATGCAGGTCC TCCACCTTCT GGGCCCCAAG 
TTGGAGGCTG ATCTGGAGAA GAAGTTCAAG GTGGCAAAAG CTCGGCTAGA AGAAACAGAC 
CGGAGGACGG CAAAGGATGT GGTGGAGAAT GGCGAGACTG CTGACCAGAC CCTGTCTCTG 
ATGGAGCAGC TCCGGGGGGA GGCCCTTAAG TTCCACAAGC CTGGTGAGAA CTACAAGACC 
CCAGGCTATG TGGTCACTCC ACACACCATG AATCTACTAA AGCAGCACCT GGAGATTACT 
GGTGGGCAGG TACGTACCCG GTTCCCGCCA GAACCCAATG GAATCCTGCA TATTGGACAT 
GCCAAAGCCA TCAATTTCAA CTTTGGCTAT GCCAAGGCCA ACAATGGCAT CTGTTTTCTG 
CGTTTTGATG ACACCAACCC TGAGAAGGAG GAAGCAAAGT TCTTCACGGC CATCTGTGAC 
ATGGTAGCCT GGCTAGGCTA CACACCTTAC AAAGTCACAT ATGCGTCTGA CTATTTTGAC 
CAGCTATATG CGTGGGCTGT GGAGCTCATC CGCAGGGGTC TGGCTTATGT GTGCCACCAG 
CGAGGAGAGG AGCTCAAAGG CCATAATACT CTGCCTTCAC CCTGGAGAGA CCGTCCCATG 
GAGGAGTCAC TGCTGCTCTT TGAGGCAATG CGCAAGGGCA AGTTTTCAGA GGGCGAGGCC 
ACACTACGGA TGAAGCTGGT GATGGAGGAT GGCAAGATGG ACCCTGTAGC CTATCGAGTC 
AAGTATACAC CACACCACCG CACAGGGGAC AAATGGTGCA TCTATCCCAC CTACGACTAC 
ACACACTGCC TCTGTGACTC CATCGAGCAC ATCACTCACT CACTCTGCAC CAAGGAATTC 
CAGGCCCGAC GCTCTTCCTA CTTCTGGCTT TGCAATGCAC TGGACGTCTA TTGCCCTGTG 
CAGTGGGAGT ATGGCCGCCT CAACCTGCAC TATGCTGTTG TCTCTAAGAG GAAGATCCTC 
CAGCTTGTAG CAACTGGTGC TGTGCGGGAC TGGGATGACC CACGGCTCTT TACACTCACG 
GCCCTGCGAC GGCGGGGCTT CCCACCTGAG GCCATCAACA ACTTCTGTGC CCGGGTGGGA 
GTGACTGTGG CACAAACCAC AATGGAGCCA CATCTTCTAG AAGCCTGTGT GCGTGATGTG 
CTGAATGACA CAGCCCCACG AGCCATGGCT GTGCTGGAGT CACTACGGGT CATCATCACC 
AACTTTCCTG CTGCCAAGTC CTTGGACATC CAGGTGCCCA ACTTCCCAGC TGATGAGACC 
AAAGGCTTCC ATCAGGTTCC CTTTGCACCC ATTGTCTTCA TTGAGAGGAC TGACTTCAAG 
GAGGAGCCAG AGCCAGGATT TAAGCGCCTG GCTTGGGGCC AGCCTGTGGG CCTGAGGCAT 
ACAGGCTACG TCATTGAGCT GCAGCATGTT GTCAAGGGCC CCAGTGGTTG TGTAGAGAGT 
CTGGAGGTGA CCTGCAGACG GGCAGATGCT GGAGAGAAGC CAAAGGCCTT TATTCACTGG 
GTGTCACAGC CTTTGATGTG TGAGGTTCGC CTCTATGAGC GACTATTCCA GCACAAGAAC 
CCTGAAGATC CTACTGAGGT GCCTGGTGGA TTTTTAAGTG ACCTGAACCT GCTTGTCTTT 
AACCGAACTG TCACACTGAA GGAAGACCCA GGAAAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862454.2
CDS	26..2227
Translation	MAALDSLSLFTSLGLSEQKARETLKNSALSAQLREAATQAQQTLGSTIDKATGILLYGLASRLRDTRRLSFLVSYIASKKIHTEPQLSAALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEVDMQVLHLLGPKLEADLEKKFKVAKARLEETDRRTAKDVVENGETADQTLSLMEQLRGEALKFHKPGENYKTPGYVVTPHTMNLLKQHLEITGGQVRTRFPPEPNGILHIGHAKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAICDMVAWLGYTPYKVTYASDYFDQLYAWAVELIRRGLAYVCHQRGEELKGHNTLPSPWRDRPMEESLLLFEAMRKGKFSEGEATLRMKLVMEDGKMDPVAYRVKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLLVFNRTVTLKEDPGKV

Target ORF information:

RefSeq Version	XM_017006965.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006965.2

ORF Insert Sequence:

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CAGTAAGAAG 
ATCCACACTG AGCCCCAGCT AAGCGCTGCC CTTGAGTATG TGCGGAGTCA CCCCTTGGAC 
CCCATCGACA CTGTGGACTT CGAGCGGGAA TGTGGCGTGG GTGTCATTGT GACCCCAGAG 
CAGATTGAGG AGGCTGTGGA GGCTGCTATT AACAGGCACC GGCCCCAGCT CCTGGTGGAA 
CGTTACCATT TCAACATGGG GCTGCTGATG GGAGAGGCTC GGGCTGTGCT GAAGTGGGCA 
GATGGCAAAA TGATCAAGAA TGAAGTGGAC ATGCAGGTCC TCCACCTTCT GGGCCCCAAG 
TTGGAGGCTG ATCTGGAGAA GAAGTTCAAG GTGGCAAAAG CTCGGCTAGA AGAAACAGAC 
CGGAGGACGG CAAAGGATGT GGTGGAGAAT GGCGAGACTG CTGACCAGAC CCTGTCTCTG 
ATGGAGCAGC TCCGGGGGGA GGCCCTTAAG TTCCACAAGC CTGGTGAGAA CTACAAGACC 
CCAGGCTATG TGGTCACTCC ACACACCATG AATCTACTAA AGCAGCACCT GGAGATTACT 
GGTGGGCAGG TACGTACCCG GTTCCCGCCA GAACCCAATG GAATCCTGCA TATTGGACAT 
GCCAAAGCCA TCAATTTCAA CTTTGGCTAT GCCAAGGCCA ACAATGGCAT CTGTTTTCTG 
CGTTTTGATG ACACCAACCC TGAGAAGGAG GAAGCAAAGT TCTTCACGGC CATCTGTGAC 
ATGGTAGCCT GGCTAGGCTA CACACCTTAC AAAGTCACAT ATGCGTCTGA CTATTTTGAC 
CAGCTATATG CGTGGGCTGT GGAGCTCATC CGCAGGGGTC TGGCTTATGT GTGCCACCAG 
CGAGGAGAGG AGCTCAAAGG CCATAATACT CTGCCTTCAC CCTGGAGAGA CCGTCCCATG 
GAGGAGTCAC TGCTGCTCTT TGAGGCAATG CGCAAGGGCA AGTTTTCAGA GGGCGAGGCC 
ACACTACGGA TGAAGCTGGT GATGGAGGAT GGCAAGATGG ACCCTGTAGC CTATCGAGTC 
AAGTATACAC CACACCACCG CACAGGGGAC AAATGGTGCA TCTATCCCAC CTACGACTAC 
ACACACTGCC TCTGTGACTC CATCGAGCAC ATCACTCACT CACTCTGCAC CAAGGAATTC 
CAGGCCCGAC GCTCTTCCTA CTTCTGGCTT TGCAATGCAC TGGACGTCTA TTGCCCTGTG 
CAGTGGGAGT ATGGCCGCCT CAACCTGCAC TATGCTGTTG TCTCTAAGAG GAAGATCCTC 
CAGCTTGTAG CAACTGGTGC TGTGCGGGAC TGGGATGACC CACGGCTCTT TACACTCACG 
GCCCTGCGAC GGCGGGGCTT CCCACCTGAG GCCATCAACA ACTTCTGTGC CCGGGTGGGA 
GTGACTGTGG CACAAACCAC AATGGAGCCA CATCTTCTAG AAGCCTGTGT GCGTGATGTG 
CTGAATGACA CAGCCCCACG AGCCATGGCT GTGCTGGAGT CACTACGGGT CATCATCACC 
AACTTTCCTG CTGCCAAGTC CTTGGACATC CAGGTGCCCA ACTTCCCAGC TGATGAGACC 
AAAGGCTTCC ATCAGGTTCC CTTTGCACCC ATTGTCTTCA TTGAGAGGAC TGACTTCAAG 
GAGGAGCCAG AGCCAGGATT TAAGCGCCTG GCTTGGGGCC AGCCTGTGGG CCTGAGGCAT 
ACAGGCTACG TCATTGAGCT GCAGCATGTT GTCAAGGGCC CCAGTGGTTG TGTAGAGAGT 
CTGGAGGTGA CCTGCAGACG GGCAGATGCT GGAGAGAAGC CAAAGGCCTT TATTCACTGG 
GTGTCACAGC CTTTGATGTG TGAGGTTCGC CTCTATGAGC GACTATTCCA GCACAAGAAC 
CCTGAAGATC CTACTGAGGT GCCTGGTGGA TTTTTAAGTG ACCTGAACCT GCTTGTCTTT 
AACCGAACTG TCACACTGAA GGAAGACCCA GGAAAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

QARS ( XM_017006965.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862454.1 Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant X1, mRNA.

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CloneID	OHu94441
Clone ID Related Accession (Same CDS sequence)	XM_017006965.1
Accession Version	XM_017006965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	glutamine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCT GCAGACTGGG GCCTAAGTTT CTTTTAGTTT CCGGTGTCTC TGCAATGGCG 
GCTCTAGACT CCCTGTCGCT CTTCACTAGC CTCGGCCTGA GCGAGCAGAA GGCCCGCGAG 
ACGCTCAAGA ACTCGGCTCT GAGCGCGCAG CTGCGCGAGG CCGCTACTCA GGCTCAGCAG 
ACCCTGGGTT CCACCATTGA CAAAGCTACC GGGATCCTGT TATATGGCTT GGCCTCCCGA 
CTCAGGGATA CCCGGCGTCT CTCCTTCCTT GTAAGCTACA TAGCCAGTAA GAAGATCCAC 
ACTGAGCCCC AGCTAAGCGC TGCCCTTGAG TATGTGCGGA GTCACCCCTT GGACCCCATC 
GACACTGTGG ACTTCGAGCG GGAATGTGGC GTGGGTGTCA TTGTGACCCC AGAGCAGATT 
GAGGAGGCTG TGGAGGCTGC TATTAACAGG CACCGGCCCC AGCTCCTGGT GGAACGTTAC 
CATTTCAACA TGGGGCTGCT GATGGGAGAG GCTCGGGCTG TGCTGAAGTG GGCAGATGGC 
AAAATGATCA AGAATGAAGT GGACATGCAG GTCCTCCACC TTCTGGGCCC CAAGTTGGAG 
GCTGATCTGG AGAAGAAGTT CAAGGTGGCA AAAGCTCGGC TAGAAGAAAC AGACCGGAGG 
ACGGCAAAGG ATGTGGTGGA GAATGGCGAG ACTGCTGACC AGACCCTGTC TCTGATGGAG 
CAGCTCCGGG GGGAGGCCCT TAAGTTCCAC AAGCCTGGTG AGAACTACAA GACCCCAGGC 
TATGTGGTCA CTCCACACAC CATGAATCTA CTAAAGCAGC ACCTGGAGAT TACTGGTGGG 
CAGGTACGTA CCCGGTTCCC GCCAGAACCC AATGGAATCC TGCATATTGG ACATGCCAAA 
GCCATCAATT TCAACTTTGG CTATGCCAAG GCCAACAATG GCATCTGTTT TCTGCGTTTT 
GATGACACCA ACCCTGAGAA GGAGGAAGCA AAGTTCTTCA CGGCCATCTG TGACATGGTA 
GCCTGGCTAG GCTACACACC TTACAAAGTC ACATATGCGT CTGACTATTT TGACCAGCTA 
TATGCGTGGG CTGTGGAGCT CATCCGCAGG GGTCTGGCTT ATGTGTGCCA CCAGCGAGGA 
GAGGAGCTCA AAGGCCATAA TACTCTGCCT TCACCCTGGA GAGACCGTCC CATGGAGGAG 
TCACTGCTGC TCTTTGAGGC AATGCGCAAG GGCAAGTTTT CAGAGGGCGA GGCCACACTA 
CGGATGAAGC TGGTGATGGA GGATGGCAAG ATGGACCCTG TAGCCTATCG AGTCAAGTAT 
ACACCACACC ACCGCACAGG GGACAAATGG TGCATCTATC CCACCTACGA CTACACACAC 
TGCCTCTGTG ACTCCATCGA GCACATCACT CACTCACTCT GCACCAAGGA ATTCCAGGCC 
CGACGCTCTT CCTACTTCTG GCTTTGCAAT GCACTGGACG TCTATTGCCC TGTGCAGTGG 
GAGTATGGCC GCCTCAACCT GCACTATGCT GTTGTCTCTA AGAGGAAGAT CCTCCAGCTT 
GTAGCAACTG GTGCTGTGCG GGACTGGGAT GACCCACGGC TCTTTACACT CACGGCCCTG 
CGACGGCGGG GCTTCCCACC TGAGGCCATC AACAACTTCT GTGCCCGGGT GGGAGTGACT 
GTGGCACAAA CCACAATGGA GCCACATCTT CTAGAAGCCT GTGTGCGTGA TGTGCTGAAT 
GACACAGCCC CACGAGCCAT GGCTGTGCTG GAGTCACTAC GGGTCATCAT CACCAACTTT 
CCTGCTGCCA AGTCCTTGGA CATCCAGGTG CCCAACTTCC CAGCTGATGA GACCAAAGGC 
TTCCATCAGG TTCCCTTTGC ACCCATTGTC TTCATTGAGA GGACTGACTT CAAGGAGGAG 
CCAGAGCCAG GATTTAAGCG CCTGGCTTGG GGCCAGCCTG TGGGCCTGAG GCATACAGGC 
TACGTCATTG AGCTGCAGCA TGTTGTCAAG GGCCCCAGTG GTTGTGTAGA GAGTCTGGAG 
GTGACCTGCA GACGGGCAGA TGCTGGAGAG AAGCCAAAGG CCTTTATTCA CTGGGTGTCA 
CAGCCTTTGA TGTGTGAGGT TCGCCTCTAT GAGCGACTAT TCCAGCACAA GAACCCTGAA 
GATCCTACTG AGGTGCCTGG TGGATTTTTA AGTGACCTGA ACCTGCTTGT CTTTAACCGA 
ACTGTCACAC TGAAGGAAGA CCCAGGAAAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862454.1
CDS	23..2278
Translation	MPTCRLGPKFLLVSGVSAMAALDSLSLFTSLGLSEQKARETLKNSALSAQLREAATQAQQTLGSTIDKATGILLYGLASRLRDTRRLSFLVSYIASKKIHTEPQLSAALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEVDMQVLHLLGPKLEADLEKKFKVAKARLEETDRRTAKDVVENGETADQTLSLMEQLRGEALKFHKPGENYKTPGYVVTPHTMNLLKQHLEITGGQVRTRFPPEPNGILHIGHAKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAICDMVAWLGYTPYKVTYASDYFDQLYAWAVELIRRGLAYVCHQRGEELKGHNTLPSPWRDRPMEESLLLFEAMRKGKFSEGEATLRMKLVMEDGKMDPVAYRVKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLLVFNRTVTLKEDPGKV

Target ORF information:

RefSeq Version	XM_017006965.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006965.1

ORF Insert Sequence:

ATGCCGACCT GCAGACTGGG GCCTAAGTTT CTTTTAGTTT CCGGTGTCTC TGCAATGGCG 
GCTCTAGACT CCCTGTCGCT CTTCACTAGC CTCGGCCTGA GCGAGCAGAA GGCCCGCGAG 
ACGCTCAAGA ACTCGGCTCT GAGCGCGCAG CTGCGCGAGG CCGCTACTCA GGCTCAGCAG 
ACCCTGGGTT CCACCATTGA CAAAGCTACC GGGATCCTGT TATATGGCTT GGCCTCCCGA 
CTCAGGGATA CCCGGCGTCT CTCCTTCCTT GTAAGCTACA TAGCCAGTAA GAAGATCCAC 
ACTGAGCCCC AGCTAAGCGC TGCCCTTGAG TATGTGCGGA GTCACCCCTT GGACCCCATC 
GACACTGTGG ACTTCGAGCG GGAATGTGGC GTGGGTGTCA TTGTGACCCC AGAGCAGATT 
GAGGAGGCTG TGGAGGCTGC TATTAACAGG CACCGGCCCC AGCTCCTGGT GGAACGTTAC 
CATTTCAACA TGGGGCTGCT GATGGGAGAG GCTCGGGCTG TGCTGAAGTG GGCAGATGGC 
AAAATGATCA AGAATGAAGT GGACATGCAG GTCCTCCACC TTCTGGGCCC CAAGTTGGAG 
GCTGATCTGG AGAAGAAGTT CAAGGTGGCA AAAGCTCGGC TAGAAGAAAC AGACCGGAGG 
ACGGCAAAGG ATGTGGTGGA GAATGGCGAG ACTGCTGACC AGACCCTGTC TCTGATGGAG 
CAGCTCCGGG GGGAGGCCCT TAAGTTCCAC AAGCCTGGTG AGAACTACAA GACCCCAGGC 
TATGTGGTCA CTCCACACAC CATGAATCTA CTAAAGCAGC ACCTGGAGAT TACTGGTGGG 
CAGGTACGTA CCCGGTTCCC GCCAGAACCC AATGGAATCC TGCATATTGG ACATGCCAAA 
GCCATCAATT TCAACTTTGG CTATGCCAAG GCCAACAATG GCATCTGTTT TCTGCGTTTT 
GATGACACCA ACCCTGAGAA GGAGGAAGCA AAGTTCTTCA CGGCCATCTG TGACATGGTA 
GCCTGGCTAG GCTACACACC TTACAAAGTC ACATATGCGT CTGACTATTT TGACCAGCTA 
TATGCGTGGG CTGTGGAGCT CATCCGCAGG GGTCTGGCTT ATGTGTGCCA CCAGCGAGGA 
GAGGAGCTCA AAGGCCATAA TACTCTGCCT TCACCCTGGA GAGACCGTCC CATGGAGGAG 
TCACTGCTGC TCTTTGAGGC AATGCGCAAG GGCAAGTTTT CAGAGGGCGA GGCCACACTA 
CGGATGAAGC TGGTGATGGA GGATGGCAAG ATGGACCCTG TAGCCTATCG AGTCAAGTAT 
ACACCACACC ACCGCACAGG GGACAAATGG TGCATCTATC CCACCTACGA CTACACACAC 
TGCCTCTGTG ACTCCATCGA GCACATCACT CACTCACTCT GCACCAAGGA ATTCCAGGCC 
CGACGCTCTT CCTACTTCTG GCTTTGCAAT GCACTGGACG TCTATTGCCC TGTGCAGTGG 
GAGTATGGCC GCCTCAACCT GCACTATGCT GTTGTCTCTA AGAGGAAGAT CCTCCAGCTT 
GTAGCAACTG GTGCTGTGCG GGACTGGGAT GACCCACGGC TCTTTACACT CACGGCCCTG 
CGACGGCGGG GCTTCCCACC TGAGGCCATC AACAACTTCT GTGCCCGGGT GGGAGTGACT 
GTGGCACAAA CCACAATGGA GCCACATCTT CTAGAAGCCT GTGTGCGTGA TGTGCTGAAT 
GACACAGCCC CACGAGCCAT GGCTGTGCTG GAGTCACTAC GGGTCATCAT CACCAACTTT 
CCTGCTGCCA AGTCCTTGGA CATCCAGGTG CCCAACTTCC CAGCTGATGA GACCAAAGGC 
TTCCATCAGG TTCCCTTTGC ACCCATTGTC TTCATTGAGA GGACTGACTT CAAGGAGGAG 
CCAGAGCCAG GATTTAAGCG CCTGGCTTGG GGCCAGCCTG TGGGCCTGAG GCATACAGGC 
TACGTCATTG AGCTGCAGCA TGTTGTCAAG GGCCCCAGTG GTTGTGTAGA GAGTCTGGAG 
GTGACCTGCA GACGGGCAGA TGCTGGAGAG AAGCCAAAGG CCTTTATTCA CTGGGTGTCA 
CAGCCTTTGA TGTGTGAGGT TCGCCTCTAT GAGCGACTAT TCCAGCACAA GAACCCTGAA 
GATCCTACTG AGGTGCCTGG TGGATTTTTA AGTGACCTGA ACCTGCTTGT CTTTAACCGA 
ACTGTCACAC TGAAGGAAGA CCCAGGAAAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

QARS ( NM_005051.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005042.1 Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17745
Clone ID Related Accession (Same CDS sequence)	NM_005051.2 , NM_005051.3
Accession Version	NM_005051.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514304000000
Organism	Homo sapiens(human)
Product	glutamine--tRNA ligase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM560535.1, AK302867.1 and BC000394.2. This sequence is a reference standard in the RefSeqGene project. On Jan 12, 2013 this sequence version replaced NM_005051.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000394.2, SRR1803617.92961.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:22223895) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CAGTAAGAAG 
ATCCACACTG AGCCCCAGCT AAGCGCTGCC CTTGAGTATG TGCGGAGTCA CCCCTTGGAC 
CCCATCGACA CTGTGGACTT CGAGCGGGAA TGTGGCGTGG GTGTCATTGT GACCCCAGAG 
CAGATTGAGG AGGCTGTGGA GGCTGCTATT AACAGGCACC GGCCCCAGCT CCTGGTGGAA 
CGTTACCATT TCAACATGGG GCTGCTGATG GGAGAGGCTC GGGCTGTGCT GAAGTGGGCA 
GATGGCAAAA TGATCAAGAA TGAAGTGGAC ATGCAGGTCC TCCACCTTCT GGGCCCCAAG 
TTGGAGGCTG ATCTGGAGAA GAAGTTCAAG GTGGCAAAAG CTCGGCTAGA AGAAACAGAC 
CGGAGGACGG CAAAGGATGT GGTGGAGAAT GGCGAGACTG CTGACCAGAC CCTGTCTCTG 
ATGGAGCAGC TCCGGGGGGA GGCCCTTAAG TTCCACAAGC CTGGTGAGAA CTACAAGACC 
CCAGGCTATG TGGTCACTCC ACACACCATG AATCTACTAA AGCAGCACCT GGAGATTACT 
GGTGGGCAGG TACGTACCCG GTTCCCGCCA GAACCCAATG GAATCCTGCA TATTGGACAT 
GCCAAAGCCA TCAATTTCAA CTTTGGCTAT GCCAAGGCCA ACAATGGCAT CTGTTTTCTG 
CGTTTTGATG ACACCAACCC TGAGAAGGAG GAAGCAAAGT TCTTCACGGC CATCTGTGAC 
ATGGTAGCCT GGCTAGGCTA CACACCTTAC AAAGTCACAT ATGCGTCTGA CTATTTTGAC 
CAGCTATATG CGTGGGCTGT GGAGCTCATC CGCAGGGGTC TGGCTTATGT GTGCCACCAG 
CGAGGAGAGG AGCTCAAAGG CCATAATACT CTGCCTTCAC CCTGGAGAGA CCGTCCCATG 
GAGGAGTCAC TGCTGCTCTT TGAGGCAATG CGCAAGGGCA AGTTTTCAGA GGGCGAGGCC 
ACACTACGGA TGAAGCTGGT GATGGAGGAT GGCAAGATGG ACCCTGTAGC CTATCGAGTC 
AAGTATACAC CACACCACCG CACAGGGGAC AAATGGTGCA TCTATCCCAC CTACGACTAC 
ACACACTGCC TCTGTGACTC CATCGAGCAC ATCACTCACT CACTCTGCAC CAAGGAATTC 
CAGGCCCGAC GCTCTTCCTA CTTCTGGCTT TGCAATGCAC TGGACGTCTA TTGCCCTGTG 
CAGTGGGAGT ATGGCCGCCT CAACCTGCAC TATGCTGTTG TCTCTAAGAG GAAGATCCTC 
CAGCTTGTAG CAACTGGTGC TGTGCGGGAC TGGGATGACC CACGGCTCTT TACACTCACG 
GCCCTGCGAC GGCGGGGCTT CCCACCTGAG GCCATCAACA ACTTCTGTGC CCGGGTGGGA 
GTGACTGTGG CACAAACCAC AATGGAGCCA CATCTTCTAG AAGCCTGTGT GCGTGATGTG 
CTGAATGACA CAGCCCCACG AGCCATGGCT GTGCTGGAGT CACTACGGGT CATCATCACC 
AACTTTCCTG CTGCCAAGTC CTTGGACATC CAGGTGCCCA ACTTCCCAGC TGATGAGACC 
AAAGGCTTCC ATCAGGTTCC CTTTGCACCC ATTGTCTTCA TTGAGAGGAC TGACTTCAAG 
GAGGAGCCAG AGCCAGGATT TAAGCGCCTG GCTTGGGGCC AGCCTGTGGG CCTGAGGCAT 
ACAGGCTACG TCATTGAGCT GCAGCATGTT GTCAAGGGCC CCAGTGGTTG TGTAGAGAGT 
CTGGAGGTGA CCTGCAGACG GGCAGATGCT GGAGAGAAGC CAAAGGCCTT TATTCACTGG 
GTGTCACAGC CTTTGATGTG TGAGGTTCGC CTCTATGAGC GACTATTCCA GCACAAGAAC 
CCTGAAGATC CTACTGAGGT GCCTGGTGGA TTTTTAAGTG ACCTGAACCT GGCATCACTA 
CACGTGGTGG ATGCAGCATT AGTGGACTGC TCTGTGGCCC TGGCAAAACC CTTCGACAAG 
TTCCAGTTTG AGCGTCTTGG ATATTTCTCC GTGGATCCAG ACAGCCATCA GGGAAAGCTT 
GTCTTTAACC GAACTGTCAC ACTGAAGGAA GACCCAGGAA AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005042.1
CDS	397..2724
Translation	MAALDSLSLFTSLGLSEQKARETLKNSALSAQLREAATQAQQTLGSTIDKATGILLYGLASRLRDTRRLSFLVSYIASKKIHTEPQLSAALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEVDMQVLHLLGPKLEADLEKKFKVAKARLEETDRRTAKDVVENGETADQTLSLMEQLRGEALKFHKPGENYKTPGYVVTPHTMNLLKQHLEITGGQVRTRFPPEPNGILHIGHAKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAICDMVAWLGYTPYKVTYASDYFDQLYAWAVELIRRGLAYVCHQRGEELKGHNTLPSPWRDRPMEESLLLFEAMRKGKFSEGEATLRMKLVMEDGKMDPVAYRVKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

Target ORF information:

RefSeq Version	NM_005051.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminyl-tRNA synthetase (QARS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005051.2

ORF Insert Sequence:

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CAGTAAGAAG 
ATCCACACTG AGCCCCAGCT AAGCGCTGCC CTTGAGTATG TGCGGAGTCA CCCCTTGGAC 
CCCATCGACA CTGTGGACTT CGAGCGGGAA TGTGGCGTGG GTGTCATTGT GACCCCAGAG 
CAGATTGAGG AGGCTGTGGA GGCTGCTATT AACAGGCACC GGCCCCAGCT CCTGGTGGAA 
CGTTACCATT TCAACATGGG GCTGCTGATG GGAGAGGCTC GGGCTGTGCT GAAGTGGGCA 
GATGGCAAAA TGATCAAGAA TGAAGTGGAC ATGCAGGTCC TCCACCTTCT GGGCCCCAAG 
TTGGAGGCTG ATCTGGAGAA GAAGTTCAAG GTGGCAAAAG CTCGGCTAGA AGAAACAGAC 
CGGAGGACGG CAAAGGATGT GGTGGAGAAT GGCGAGACTG CTGACCAGAC CCTGTCTCTG 
ATGGAGCAGC TCCGGGGGGA GGCCCTTAAG TTCCACAAGC CTGGTGAGAA CTACAAGACC 
CCAGGCTATG TGGTCACTCC ACACACCATG AATCTACTAA AGCAGCACCT GGAGATTACT 
GGTGGGCAGG TACGTACCCG GTTCCCGCCA GAACCCAATG GAATCCTGCA TATTGGACAT 
GCCAAAGCCA TCAATTTCAA CTTTGGCTAT GCCAAGGCCA ACAATGGCAT CTGTTTTCTG 
CGTTTTGATG ACACCAACCC TGAGAAGGAG GAAGCAAAGT TCTTCACGGC CATCTGTGAC 
ATGGTAGCCT GGCTAGGCTA CACACCTTAC AAAGTCACAT ATGCGTCTGA CTATTTTGAC 
CAGCTATATG CGTGGGCTGT GGAGCTCATC CGCAGGGGTC TGGCTTATGT GTGCCACCAG 
CGAGGAGAGG AGCTCAAAGG CCATAATACT CTGCCTTCAC CCTGGAGAGA CCGTCCCATG 
GAGGAGTCAC TGCTGCTCTT TGAGGCAATG CGCAAGGGCA AGTTTTCAGA GGGCGAGGCC 
ACACTACGGA TGAAGCTGGT GATGGAGGAT GGCAAGATGG ACCCTGTAGC CTATCGAGTC 
AAGTATACAC CACACCACCG CACAGGGGAC AAATGGTGCA TCTATCCCAC CTACGACTAC 
ACACACTGCC TCTGTGACTC CATCGAGCAC ATCACTCACT CACTCTGCAC CAAGGAATTC 
CAGGCCCGAC GCTCTTCCTA CTTCTGGCTT TGCAATGCAC TGGACGTCTA TTGCCCTGTG 
CAGTGGGAGT ATGGCCGCCT CAACCTGCAC TATGCTGTTG TCTCTAAGAG GAAGATCCTC 
CAGCTTGTAG CAACTGGTGC TGTGCGGGAC TGGGATGACC CACGGCTCTT TACACTCACG 
GCCCTGCGAC GGCGGGGCTT CCCACCTGAG GCCATCAACA ACTTCTGTGC CCGGGTGGGA 
GTGACTGTGG CACAAACCAC AATGGAGCCA CATCTTCTAG AAGCCTGTGT GCGTGATGTG 
CTGAATGACA CAGCCCCACG AGCCATGGCT GTGCTGGAGT CACTACGGGT CATCATCACC 
AACTTTCCTG CTGCCAAGTC CTTGGACATC CAGGTGCCCA ACTTCCCAGC TGATGAGACC 
AAAGGCTTCC ATCAGGTTCC CTTTGCACCC ATTGTCTTCA TTGAGAGGAC TGACTTCAAG 
GAGGAGCCAG AGCCAGGATT TAAGCGCCTG GCTTGGGGCC AGCCTGTGGG CCTGAGGCAT 
ACAGGCTACG TCATTGAGCT GCAGCATGTT GTCAAGGGCC CCAGTGGTTG TGTAGAGAGT 
CTGGAGGTGA CCTGCAGACG GGCAGATGCT GGAGAGAAGC CAAAGGCCTT TATTCACTGG 
GTGTCACAGC CTTTGATGTG TGAGGTTCGC CTCTATGAGC GACTATTCCA GCACAAGAAC 
CCTGAAGATC CTACTGAGGT GCCTGGTGGA TTTTTAAGTG ACCTGAACCT GGCATCACTA 
CACGTGGTGG ATGCAGCATT AGTGGACTGC TCTGTGGCCC TGGCAAAACC CTTCGACAAG 
TTCCAGTTTG AGCGTCTTGG ATATTTCTCC GTGGATCCAG ACAGCCATCA GGGAAAGCTT 
GTCTTTAACC GAACTGTCAC ACTGAAGGAA GACCCAGGAA AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

QARS ( NM_005051.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005042.1 Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17745
Clone ID Related Accession (Same CDS sequence)	NM_005051.2 , NM_005051.3
Accession Version	NM_005051.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	glutamine--tRNA ligase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM560535.1 and BC000394.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005051.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290523.1, SRR3476690.769775.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:22223895) MANE Ensembl match :: ENST00000306125.12/ ENSP00000307567.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CAGTAAGAAG 
ATCCACACTG AGCCCCAGCT AAGCGCTGCC CTTGAGTATG TGCGGAGTCA CCCCTTGGAC 
CCCATCGACA CTGTGGACTT CGAGCGGGAA TGTGGCGTGG GTGTCATTGT GACCCCAGAG 
CAGATTGAGG AGGCTGTGGA GGCTGCTATT AACAGGCACC GGCCCCAGCT CCTGGTGGAA 
CGTTACCATT TCAACATGGG GCTGCTGATG GGAGAGGCTC GGGCTGTGCT GAAGTGGGCA 
GATGGCAAAA TGATCAAGAA TGAAGTGGAC ATGCAGGTCC TCCACCTTCT GGGCCCCAAG 
TTGGAGGCTG ATCTGGAGAA GAAGTTCAAG GTGGCAAAAG CTCGGCTAGA AGAAACAGAC 
CGGAGGACGG CAAAGGATGT GGTGGAGAAT GGCGAGACTG CTGACCAGAC CCTGTCTCTG 
ATGGAGCAGC TCCGGGGGGA GGCCCTTAAG TTCCACAAGC CTGGTGAGAA CTACAAGACC 
CCAGGCTATG TGGTCACTCC ACACACCATG AATCTACTAA AGCAGCACCT GGAGATTACT 
GGTGGGCAGG TACGTACCCG GTTCCCGCCA GAACCCAATG GAATCCTGCA TATTGGACAT 
GCCAAAGCCA TCAATTTCAA CTTTGGCTAT GCCAAGGCCA ACAATGGCAT CTGTTTTCTG 
CGTTTTGATG ACACCAACCC TGAGAAGGAG GAAGCAAAGT TCTTCACGGC CATCTGTGAC 
ATGGTAGCCT GGCTAGGCTA CACACCTTAC AAAGTCACAT ATGCGTCTGA CTATTTTGAC 
CAGCTATATG CGTGGGCTGT GGAGCTCATC CGCAGGGGTC TGGCTTATGT GTGCCACCAG 
CGAGGAGAGG AGCTCAAAGG CCATAATACT CTGCCTTCAC CCTGGAGAGA CCGTCCCATG 
GAGGAGTCAC TGCTGCTCTT TGAGGCAATG CGCAAGGGCA AGTTTTCAGA GGGCGAGGCC 
ACACTACGGA TGAAGCTGGT GATGGAGGAT GGCAAGATGG ACCCTGTAGC CTATCGAGTC 
AAGTATACAC CACACCACCG CACAGGGGAC AAATGGTGCA TCTATCCCAC CTACGACTAC 
ACACACTGCC TCTGTGACTC CATCGAGCAC ATCACTCACT CACTCTGCAC CAAGGAATTC 
CAGGCCCGAC GCTCTTCCTA CTTCTGGCTT TGCAATGCAC TGGACGTCTA TTGCCCTGTG 
CAGTGGGAGT ATGGCCGCCT CAACCTGCAC TATGCTGTTG TCTCTAAGAG GAAGATCCTC 
CAGCTTGTAG CAACTGGTGC TGTGCGGGAC TGGGATGACC CACGGCTCTT TACACTCACG 
GCCCTGCGAC GGCGGGGCTT CCCACCTGAG GCCATCAACA ACTTCTGTGC CCGGGTGGGA 
GTGACTGTGG CACAAACCAC AATGGAGCCA CATCTTCTAG AAGCCTGTGT GCGTGATGTG 
CTGAATGACA CAGCCCCACG AGCCATGGCT GTGCTGGAGT CACTACGGGT CATCATCACC 
AACTTTCCTG CTGCCAAGTC CTTGGACATC CAGGTGCCCA ACTTCCCAGC TGATGAGACC 
AAAGGCTTCC ATCAGGTTCC CTTTGCACCC ATTGTCTTCA TTGAGAGGAC TGACTTCAAG 
GAGGAGCCAG AGCCAGGATT TAAGCGCCTG GCTTGGGGCC AGCCTGTGGG CCTGAGGCAT 
ACAGGCTACG TCATTGAGCT GCAGCATGTT GTCAAGGGCC CCAGTGGTTG TGTAGAGAGT 
CTGGAGGTGA CCTGCAGACG GGCAGATGCT GGAGAGAAGC CAAAGGCCTT TATTCACTGG 
GTGTCACAGC CTTTGATGTG TGAGGTTCGC CTCTATGAGC GACTATTCCA GCACAAGAAC 
CCTGAAGATC CTACTGAGGT GCCTGGTGGA TTTTTAAGTG ACCTGAACCT GGCATCACTA 
CACGTGGTGG ATGCAGCATT AGTGGACTGC TCTGTGGCCC TGGCAAAACC CTTCGACAAG 
TTCCAGTTTG AGCGTCTTGG ATATTTCTCC GTGGATCCAG ACAGCCATCA GGGAAAGCTT 
GTCTTTAACC GAACTGTCAC ACTGAAGGAA GACCCAGGAA AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005042.1
CDS	25..2352
Translation	MAALDSLSLFTSLGLSEQKARETLKNSALSAQLREAATQAQQTLGSTIDKATGILLYGLASRLRDTRRLSFLVSYIASKKIHTEPQLSAALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEVDMQVLHLLGPKLEADLEKKFKVAKARLEETDRRTAKDVVENGETADQTLSLMEQLRGEALKFHKPGENYKTPGYVVTPHTMNLLKQHLEITGGQVRTRFPPEPNGILHIGHAKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAICDMVAWLGYTPYKVTYASDYFDQLYAWAVELIRRGLAYVCHQRGEELKGHNTLPSPWRDRPMEESLLLFEAMRKGKFSEGEATLRMKLVMEDGKMDPVAYRVKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

Target ORF information:

RefSeq Version	NM_005051.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutaminyl-tRNA synthetase 1 (QARS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005051.3

ORF Insert Sequence:

ATGGCGGCTC TAGACTCCCT GTCGCTCTTC ACTAGCCTCG GCCTGAGCGA GCAGAAGGCC 
CGCGAGACGC TCAAGAACTC GGCTCTGAGC GCGCAGCTGC GCGAGGCCGC TACTCAGGCT 
CAGCAGACCC TGGGTTCCAC CATTGACAAA GCTACCGGGA TCCTGTTATA TGGCTTGGCC 
TCCCGACTCA GGGATACCCG GCGTCTCTCC TTCCTTGTAA GCTACATAGC CAGTAAGAAG 
ATCCACACTG AGCCCCAGCT AAGCGCTGCC CTTGAGTATG TGCGGAGTCA CCCCTTGGAC 
CCCATCGACA CTGTGGACTT CGAGCGGGAA TGTGGCGTGG GTGTCATTGT GACCCCAGAG 
CAGATTGAGG AGGCTGTGGA GGCTGCTATT AACAGGCACC GGCCCCAGCT CCTGGTGGAA 
CGTTACCATT TCAACATGGG GCTGCTGATG GGAGAGGCTC GGGCTGTGCT GAAGTGGGCA 
GATGGCAAAA TGATCAAGAA TGAAGTGGAC ATGCAGGTCC TCCACCTTCT GGGCCCCAAG 
TTGGAGGCTG ATCTGGAGAA GAAGTTCAAG GTGGCAAAAG CTCGGCTAGA AGAAACAGAC 
CGGAGGACGG CAAAGGATGT GGTGGAGAAT GGCGAGACTG CTGACCAGAC CCTGTCTCTG 
ATGGAGCAGC TCCGGGGGGA GGCCCTTAAG TTCCACAAGC CTGGTGAGAA CTACAAGACC 
CCAGGCTATG TGGTCACTCC ACACACCATG AATCTACTAA AGCAGCACCT GGAGATTACT 
GGTGGGCAGG TACGTACCCG GTTCCCGCCA GAACCCAATG GAATCCTGCA TATTGGACAT 
GCCAAAGCCA TCAATTTCAA CTTTGGCTAT GCCAAGGCCA ACAATGGCAT CTGTTTTCTG 
CGTTTTGATG ACACCAACCC TGAGAAGGAG GAAGCAAAGT TCTTCACGGC CATCTGTGAC 
ATGGTAGCCT GGCTAGGCTA CACACCTTAC AAAGTCACAT ATGCGTCTGA CTATTTTGAC 
CAGCTATATG CGTGGGCTGT GGAGCTCATC CGCAGGGGTC TGGCTTATGT GTGCCACCAG 
CGAGGAGAGG AGCTCAAAGG CCATAATACT CTGCCTTCAC CCTGGAGAGA CCGTCCCATG 
GAGGAGTCAC TGCTGCTCTT TGAGGCAATG CGCAAGGGCA AGTTTTCAGA GGGCGAGGCC 
ACACTACGGA TGAAGCTGGT GATGGAGGAT GGCAAGATGG ACCCTGTAGC CTATCGAGTC 
AAGTATACAC CACACCACCG CACAGGGGAC AAATGGTGCA TCTATCCCAC CTACGACTAC 
ACACACTGCC TCTGTGACTC CATCGAGCAC ATCACTCACT CACTCTGCAC CAAGGAATTC 
CAGGCCCGAC GCTCTTCCTA CTTCTGGCTT TGCAATGCAC TGGACGTCTA TTGCCCTGTG 
CAGTGGGAGT ATGGCCGCCT CAACCTGCAC TATGCTGTTG TCTCTAAGAG GAAGATCCTC 
CAGCTTGTAG CAACTGGTGC TGTGCGGGAC TGGGATGACC CACGGCTCTT TACACTCACG 
GCCCTGCGAC GGCGGGGCTT CCCACCTGAG GCCATCAACA ACTTCTGTGC CCGGGTGGGA 
GTGACTGTGG CACAAACCAC AATGGAGCCA CATCTTCTAG AAGCCTGTGT GCGTGATGTG 
CTGAATGACA CAGCCCCACG AGCCATGGCT GTGCTGGAGT CACTACGGGT CATCATCACC 
AACTTTCCTG CTGCCAAGTC CTTGGACATC CAGGTGCCCA ACTTCCCAGC TGATGAGACC 
AAAGGCTTCC ATCAGGTTCC CTTTGCACCC ATTGTCTTCA TTGAGAGGAC TGACTTCAAG 
GAGGAGCCAG AGCCAGGATT TAAGCGCCTG GCTTGGGGCC AGCCTGTGGG CCTGAGGCAT 
ACAGGCTACG TCATTGAGCT GCAGCATGTT GTCAAGGGCC CCAGTGGTTG TGTAGAGAGT 
CTGGAGGTGA CCTGCAGACG GGCAGATGCT GGAGAGAAGC CAAAGGCCTT TATTCACTGG 
GTGTCACAGC CTTTGATGTG TGAGGTTCGC CTCTATGAGC GACTATTCCA GCACAAGAAC 
CCTGAAGATC CTACTGAGGT GCCTGGTGGA TTTTTAAGTG ACCTGAACCT GGCATCACTA 
CACGTGGTGG ATGCAGCATT AGTGGACTGC TCTGTGGCCC TGGCAAAACC CTTCGACAAG 
TTCCAGTTTG AGCGTCTTGG ATATTTCTCC GTGGATCCAG ACAGCCATCA GGGAAAGCTT 
GTCTTTAACC GAACTGTCAC ACTGAAGGAA GACCCAGGAA AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Severe growth deficiency, microcephaly, intellectual disability, and characteristic facial features are due to a homozygous QARS mutation.
Neurogenetics18(3)141-146(2017 Jul)
Leshinsky-Silver E,Ling J,Wu J,Vinkler C,Yosovich K,Bahar S,Yanoov-Sharav M,Lerman-Sagie T,Lev D

Human cytomegalovirus-encoded miR-US4-1 promotes cell apoptosis and benefits discharge of infectious virus particles by targeting QARS.
Journal of biosciences41(2)183-92(2016 Jun)
Shao Y,Qi Y,Huang Y,Liu Z,Ma Y,Guo X,Jiang S,Sun Z,Ruan Q

The crystal structure of human GlnRS provides basis for the development of neurological disorders.
Nucleic acids research44(7)3420-31(2016 Apr)
Ognjenović J,Wu J,Matthies D,Baxa U,Subramaniam S,Ling J,Simonović M

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kovács IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barabási AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Mutations in the glutaminyl-tRNA synthetase gene cause early-onset epileptic encephalopathy.
Journal of human genetics60(2)97-101(2015 Feb)
Kodera H,Osaka H,Iai M,Aida N,Yamashita A,Tsurusaki Y,Nakashima M,Miyake N,Saitsu H,Matsumoto N

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