HEG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEG1
Entrez Gene ID	57493
Full Name	heart development protein with EGF like domains 1
Synonyms	HEG,MST112,MSTP112
General protein information	Preferred Names heart development protein with EGF like domains 1 Names protein HEG homolog 1 HEG homolog 1 heart of glass
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q21.2
Disorder MIM:	614182

mRNA and Protein(s)

mRNA	Protein	Name
NM_020733.1	NP_065784.1	protein HEG homolog 1 precursor
XM_005247666.1	XP_005247723.1	protein HEG homolog 1 isoform X1
NM_020733.2	NP_065784.1	protein HEG homolog 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEG1	NP_065784.1
Bos taurus (cattle)	HEG1	XP_002684860.1
Mus musculus (house mouse)	Heg1	NP_780465.4
Macaca mulatta (Rhesus monkey)	HEG1	XP_002802724.1
Rattus norvegicus (Norway rat)	Heg1	XP_006248505.1
Pan troglodytes (chimpanzee)	HEG1	XP_516708.4
Canis lupus familiaris (dog)	HEG1	XP_005639641.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1.
Matsuura R, Kaji H, Tomioka A, Sato T, Narimatsu H, Moriwaki Y, Misawa H, Imai K, Tsuji S
Scientific reports8(1)14251(2018 09)

HEG1 is a novel mucin-like membrane protein that serves as a diagnostic and therapeutic target for malignant mesothelioma.
Tsuji S, Washimi K, Kageyama T, Yamashita M, Yoshihara M, Matsuura R, Yokose T, Kameda Y, Hayashi H, Morohoshi T, Tsuura Y, Yusa T, Sato T, Togayachi A, Narimatsu H, Nagasaki T, Nakamoto K, Moriwaki Y, Misawa H, Hiroshima K, Miyagi Y, Imai K
Scientific reports745768(2017 03)

Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity.
de Kreuk BJ, Gingras AR, Knight JD, Liu JJ, Gingras AC, Ginsberg MH
eLife5e11394(2016 Jan)

Cerebral cavernous malformations arise independent of the heart of glass receptor.
Zheng X, Riant F, Bergametti F, Myers CD, Tang AT, Kleaveland B, Pan W, Yang J, Tournier-Lasserve E, Kahn ML
Stroke45(5)1505-1509(2014 May)

The structure of the ternary complex of Krev interaction trapped 1 (KRIT1) bound to both the Rap1 GTPase and the heart of glass (HEG1) cytoplasmic tail.
Gingras AR, Puzon-McLaughlin W, Ginsberg MH
The Journal of biological chemistry288(33)23639-49(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HEG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03459	NM_020733.2 Latest version!	Homo sapiens heart development protein with EGF like domains 1 (HEG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu38076	XM_005247666.1 Latest version!	Homo sapiens heart development protein with EGF like domains 1 (HEG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OHu03459	NM_020733.1	Homo sapiens heart development protein with EGF like domains 1 (HEG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEG1 ( NM_020733.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_065784.1 Homo sapiens heart development protein with EGF like domains 1 (HEG1), mRNA.

Price & Availability↑

CloneID	OHu03459
Clone ID Related Accession (Same CDS sequence)	NM_020733.1 , NM_020733.2
Accession Version	NM_020733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514217600000
Organism	Homo sapiens(human)
Product	protein HEG homolog 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR003209.1, AB033063.2, AC092983.13 and AC026342.34. On or before Jul 26, 2007 this sequence version replaced XM_087386.9, XM_001129251.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGC CGCGCGCCTC GCGGTGGCCG CCGCCGCTCC TGCTGCTGTT GCTGCCGCTG 
CTGCTGCTGC CGCCGGCGGC CCCCGGGACG CGGGACCCGC CGCCTTCCCC GGCTCGCCGC 
GCGCTGAGCC TGGCGCCCCT CGCGGGAGCG GGGCTGGAGC TGCAGCTGGA GCGCCGCCCG 
GAGCGCGAGC CGCCGCCCAC GCCGCCCCGG GAGCGCCGCG GGCCCGCGAC CCCCGGCCCC 
AGCTACAGGG CCCCTGAGCC AGGCGCCGCG ACACAGCGGG GACCCTCCGG CCGGGCCCCC 
AGAGGCGGGA GCGCGGATGC TGCCTGGAAA CATTGGCCAG AAAGTAACAC TGAGGCCCAT 
GTAGAAAACA TCACCTTCTA TCAGAATCAA GAGGACTTTT CAACAGTGTC CTCCAAAGAG 
GGCGTGATGG TTCAGACCTC TGGGAAGAGC CATGCTGCTT CGGATGCTCC AGAAAACCTC 
ACTCTACTCG CTGAAACAGC AGATGCTAGA GGAAGGAGCG GCTCTTCAAG TAGAACAAAC 
TTCACCATTT TGCCTGTTGG GTACTCACTG GAGATAGCAA CAGCTCTGAC TTCCCAGAGT 
GGCAACTTAG CCTCAGAAAG TCTTCACCTG CCATCCAGCA GTTCAGAGTT CGATGAAAGA 
ATTGCCGCTT TTCAAACAAA GAGTGGAACA GCCTCGGAGA TGGGAACAGA GAGGGCGATG 
GGGCTGTCAG AAGAATGGAC TGTGCACAGC CAAGAGGCCA CCACTTCGGC TTGGAGCCCG 
TCCTTTCTTC CTGCTTTGGA GATGGGAGAG CTGACCACGC CTTCTAGGAA GAGAAATTCC 
TCAGGACCAG ATCTCTCCTG GCTGCATTTC TACAGGACAG CAGCTTCCTC TCCTCTCTTA 
GACCTTTCCT CATCTTCTGA AAGTACAGAG AAGCTTAACA ACTCCACTGG CCTCCAGAGC 
TCCTCAGTCA GTCAAACAAA GACAATGCAT GTTGCCACCG TGTTCACTGA TGGTGGCCCG 
AGAACGCTGC GATCTTTGAC GGTCAGTCTG GGACCTGTGA GCAAGACAGA AGGCTTCCCC 
AAGGACTCCA GAATTGCCAC GACTTCATCC TCAGTCCTTC TTTCACCCTC TGCAGTGGAA 
TCGAGAAGAA ACAGTAGAGT AACTGGGAAT CCAGGGGATG AGGAATTCAT TGAACCATCC 
ACAGAAAATG AATTTGGACT TACGTCTTTG CGTTGGCAAA ATGATTCCCC AACCTTTGGA 
GAACATCAGC TTGCCAGCAG CTCTGAGGTG CAAAATGGAA GTCCCATGTC TCAGACTGAG 
ACTGTGTCTA GGTCAGTCGC ACCCATGAGA GGTGGAGAGA TCACTGCACA CTGGCTCTTG 
ACCAACAGCA CAACATCTGC AGATGTGACA GGAAGCTCTG CTTCATATCC TGAAGGTGTG 
AATGCTTCAG TGTTGACCCA GTTCTCAGAC TCTACTGTAC AGTCTGGAGG AAGTCACACA 
GCATTGGGAG ATAGGAGTTA TTCAGAGTCT TCATCTACAT CTTCCTCGGA AAGCTTGAAT 
TCATCAGCAC CACGTGGAGA ACGTTCGATC GCTGGGATTA GCTACGGTCA AGTGCGTGGC 
ACAGCTATTG AACAAAGGAC TTCCAGCGAC CACACAGACC ACACCTACCT GTCATCTACT 
TTCACCAAAG GAGAACGGGC GTTACTGTCC ATTACAGATA ACAGTTCATC CTCAGACATT 
GTGGAGAGCT CAACTTCTTA TATTAAAATC TCAAACTCTT CACATTCAGA GTATTCCTCC 
TTTTTTCATG CTCAGACTGA GAGAAGTAAC ATCTCATCCT ATGACGGGGA ATATGCTCAG 
CCTTCTACTG AGTCGCCAGT TCTGCATACA TCCAACCTTC CGTCCTACAC ACCCACCATT 
AATATGCCGA ACACTTCGGT TGTTCTGGAC ACTGATGCTG AGTTTGTTAG TGACTCCTCC 
TCCTCCTCTT CCTCCTCCTC CTCTTCTTCT TCTTCAGGGC CTCCTTTGCC TCTGCCCTCT 
GTGTCACAAT CCCACCATTT ATTTTCATCA ATTTTACCAT CAACCAGGGC CTCTGTGCAT 
CTACTAAAGT CTACCTCTGA TGCATCCACA CCATGGTCTT CCTCACCATC ACCTTTACCA 
GTATCCTTAA CGACATCTAC ATCTGCCCCA CTTTCTGTCT CACAAACAAC CTTGCCACAG 
TCATCTTCTA CCCCTGTCCT GCCCAGGGCA AGGGAGACTC CTGTGACTTC ATTTCAGACA 
TCAACAATGA CATCATTCAT GACAATGCTC CATAGTAGTC AAACTGCAGA CCTTAAGAGC 
CAGAGCACCC CACACCAAGA GAAAGTCATT ACAGAATCAA AGTCACCAAG CCTGGTGTCT 
CTGCCCACAG AGTCCACCAA AGCTGTAACA ACAAACTCTC CTTTGCCTCC ATCCTTAACA 
GAGTCCTCCA CAGAGCAAAC CCTTCCAGCC ACAAGCACCA ACTTAGCACA AATGTCTCCA 
ACTTTCACAA CTACCATTCT GAAGACCTCT CAGCCTCTTA TGACCACTCC TGGCACCCTG 
TCAAGCACAG CATCTCTGGT CACTGGCCCT ATAGCCGTAC AGACTACAGC TGGAAAACAG 
CTCTCGCTGA CCCATCCTGA AATACTAGTT CCTCAAATCT CAACAGAAGG TGGCATCAGC 
ACAGAAAGGA ACCGAGTGAT TGTGGATGCT ACCACTGGAT TGATCCCTTT GACCAGTGTA 
CCCACATCAG CAAAAGAAAT GACCACAAAG CTTGGCGTTA CAGCAGAGTA CAGCCCAGCT 
TCACGTTCCC TCGGAACATC TCCTTCTCCC CAAACCACAG TTGTTTCCAC GGCTGAAGAC 
TTGGCTCCCA AATCTGCCAC CTTTGCTGTT CAGAGCAGCA CACAGTCACC AACAACAGTG 
TCCTCTTCAG CCTCAGTCAA CAGCTGTGCT GTGAACCCTT GTCTTCACAA TGGCGAATGC 
GTCGCAGACA ACACCAGCCG TGGCTACCAC TGCAGGTGCC CGCCTTCCTG GCAAGGGGAT 
GATTGCAGTG TGGATGTGAA TGAGTGCCTG TCGAACCCCT GCCCATCCAC AGCCATGTGC 
AACAATACTC AGGGATCCTT TATCTGCAAA TGCCCGGTTG GGTACCAGTT GGAAAAAGGG 
ATATGCAATT TGGTTAGAAC CTTCGTGACA GAGTTTAAAT TAAAGAGAAC TTTTCTTAAT 
ACAACTGTGG AAAAACATTC AGACCTACAA GAAGTTGAAA ATGAGATCAC CAAAACGTTA 
AATATGTGTT TTTCAGCGTT ACCTAGTTAC ATCCGATCTA CAGTTCACGC CTCTAGGGAG 
TCCAACGCGG TGGTGATCTC ACTGCAAACA ACCTTTTCCC TGGCCTCCAA TGTGACGCTA 
TTTGACCTGG CTGATAGGAT GCAGAAATGT GTCAACTCCT GCAAGTCCTC TGCTGAGGTC 
TGCCAGCTCT TGGGATCTCA GAGGCGGATC TTTAGAGCGG GCAGCTTGTG CAAGCGGAAG 
AGTCCCGAAT GTGACAAAGA CACCTCCATC TGCACTGACC TGGACGGCGT TGCCCTGTGC 
CAGTGCAAGT CGGGATACTT TCAGTTCAAC AAGATGGACC ACTCCTGCCG AGCATGTGAA 
GATGGATATA GGCTTGAAAA TGAAACCTGC ATGAGTTGCC CATTTGGCCT TGGTGGTCTC 
AACTGTGGAA ACCCCTATCA GCTTATCACT GTGGTGATCG CAGCCGCGGG AGGTGGGCTC 
CTGCTCATCC TAGGCATCGC ACTGATTGTT ACCTGTTGCA GAAAGAATAA AAATGACATA 
AGCAAACTCA TCTTCAAAAG TGGAGATTTC CAAATGTCCC CGTATGCTGA ATACCCCAAA 
AATCCTCGCT CACAAGAATG GGGCCGAGAA GCTATTGAAA TGCATGAGAA TGGAAGTACC 
AAAAACCTCC TCCAGATGAC GGATGTGTAC TACTCGCCTA CAAGTGTAAG GAATCCAGAA 
CTTGAACGAA ACGGACTCTA CCCGGCCTAC ACTGGACTGC CAGGATCACG GCATTCTTGC 
ATTTTCCCCG GACAGTATAA CCCGTCTTTC ATCAGTGATG AAAGCAGAAG AAGAGACTAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065784.1
CDS	69..4214
Translation	MASPRASRWPPPLLLLLLPLLLLPPAAPGTRDPPPSPARRALSLAPLAGAGLELQLERRPEREPPPTPPRERRGPATPGPSYRAPEPGAATQRGPSGRAPRGGSADAAWKHWPESNTEAHVENITFYQNQEDFSTVSSKEGVMVQTSGKSHAASDAPENLTLLAETADARGRSGSSSRTNFTILPVGYSLEIATALTSQSGNLASESLHLPSSSSEFDERIAAFQTKSGTASEMGTERAMGLSEEWTVHSQEATTSAWSPSFLPALEMGELTTPSRKRNSSGPDLSWLHFYRTAASSPLLDLSSSSESTEKLNNSTGLQSSSVSQTKTMHVATVFTDGGPRTLRSLTVSLGPVSKTEGFPKDSRIATTSSSVLLSPSAVESRRNSRVTGNPGDEEFIEPSTENEFGLTSLRWQNDSPTFGEHQLASSSEVQNGSPMSQTETVSRSVAPMRGGEITAHWLLTNSTTSADVTGSSASYPEGVNASVLTQFSDSTVQSGGSHTALGDRSYSESSSTSSSESLNSSAPRGERSIAGISYGQVRGTAIEQRTSSDHTDHTYLSSTFTKGERALLSITDNSSSSDIVESSTSYIKISNSSHSEYSSFFHAQTERSNISSYDGEYAQPSTESPVLHTSNLPSYTPTINMPNTSVVLDTDAEFVSDSSSSSSSSSSSSSSGPPLPLPSVSQSHHLFSSILPSTRASVHLLKSTSDASTPWSSSPSPLPVSLTTSTSAPLSVSQTTLPQSSSTPVLPRARETPVTSFQTSTMTSFMTMLHSSQTADLKSQSTPHQEKVITESKSPSLVSLPTESTKAVTTNSPLPPSLTESSTEQTLPATSTNLAQMSPTFTTTILKTSQPLMTTPGTLSSTASLVTGPIAVQTTAGKQLSLTHPEILVPQISTEGGISTERNRVIVDATTGLIPLTSVPTSAKEMTTKLGVTAEYSPASRSLGTSPSPQTTVVSTAEDLAPKSATFAVQSSTQSPTTVSSSASVNSCAVNPCLHNGECVADNTSRGYHCRCPPSWQGDDCSVDVNECLSNPCPSTAMCNNTQGSFICKCPVGYQLEKGICNLVRTFVTEFKLKRTFLNTTVEKHSDLQEVENEITKTLNMCFSALPSYIRSTVHASRESNAVVISLQTTFSLASNVTLFDLADRMQKCVNSCKSSAEVCQLLGSQRRIFRAGSLCKRKSPECDKDTSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGIALIVTCCRKNKNDISKLIFKSGDFQMSPYAEYPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTSVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	NM_020733.1
Organism	Homo sapiens(human)
Definition	Homo sapiens heart development protein with EGF like domains 1 (HEG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020733.1

ORF Insert Sequence:

ATGGCCTCGC CGCGCGCCTC GCGGTGGCCG CCGCCGCTCC TGCTGCTGTT GCTGCCGCTG 
CTGCTGCTGC CGCCGGCGGC CCCCGGGACG CGGGACCCGC CGCCTTCCCC GGCTCGCCGC 
GCGCTGAGCC TGGCGCCCCT CGCGGGAGCG GGGCTGGAGC TGCAGCTGGA GCGCCGCCCG 
GAGCGCGAGC CGCCGCCCAC GCCGCCCCGG GAGCGCCGCG GGCCCGCGAC CCCCGGCCCC 
AGCTACAGGG CCCCTGAGCC AGGCGCCGCG ACACAGCGGG GACCCTCCGG CCGGGCCCCC 
AGAGGCGGGA GCGCGGATGC TGCCTGGAAA CATTGGCCAG AAAGTAACAC TGAGGCCCAT 
GTAGAAAACA TCACCTTCTA TCAGAATCAA GAGGACTTTT CAACAGTGTC CTCCAAAGAG 
GGCGTGATGG TTCAGACCTC TGGGAAGAGC CATGCTGCTT CGGATGCTCC AGAAAACCTC 
ACTCTACTCG CTGAAACAGC AGATGCTAGA GGAAGGAGCG GCTCTTCAAG TAGAACAAAC 
TTCACCATTT TGCCTGTTGG GTACTCACTG GAGATAGCAA CAGCTCTGAC TTCCCAGAGT 
GGCAACTTAG CCTCAGAAAG TCTTCACCTG CCATCCAGCA GTTCAGAGTT CGATGAAAGA 
ATTGCCGCTT TTCAAACAAA GAGTGGAACA GCCTCGGAGA TGGGAACAGA GAGGGCGATG 
GGGCTGTCAG AAGAATGGAC TGTGCACAGC CAAGAGGCCA CCACTTCGGC TTGGAGCCCG 
TCCTTTCTTC CTGCTTTGGA GATGGGAGAG CTGACCACGC CTTCTAGGAA GAGAAATTCC 
TCAGGACCAG ATCTCTCCTG GCTGCATTTC TACAGGACAG CAGCTTCCTC TCCTCTCTTA 
GACCTTTCCT CATCTTCTGA AAGTACAGAG AAGCTTAACA ACTCCACTGG CCTCCAGAGC 
TCCTCAGTCA GTCAAACAAA GACAATGCAT GTTGCCACCG TGTTCACTGA TGGTGGCCCG 
AGAACGCTGC GATCTTTGAC GGTCAGTCTG GGACCTGTGA GCAAGACAGA AGGCTTCCCC 
AAGGACTCCA GAATTGCCAC GACTTCATCC TCAGTCCTTC TTTCACCCTC TGCAGTGGAA 
TCGAGAAGAA ACAGTAGAGT AACTGGGAAT CCAGGGGATG AGGAATTCAT TGAACCATCC 
ACAGAAAATG AATTTGGACT TACGTCTTTG CGTTGGCAAA ATGATTCCCC AACCTTTGGA 
GAACATCAGC TTGCCAGCAG CTCTGAGGTG CAAAATGGAA GTCCCATGTC TCAGACTGAG 
ACTGTGTCTA GGTCAGTCGC ACCCATGAGA GGTGGAGAGA TCACTGCACA CTGGCTCTTG 
ACCAACAGCA CAACATCTGC AGATGTGACA GGAAGCTCTG CTTCATATCC TGAAGGTGTG 
AATGCTTCAG TGTTGACCCA GTTCTCAGAC TCTACTGTAC AGTCTGGAGG AAGTCACACA 
GCATTGGGAG ATAGGAGTTA TTCAGAGTCT TCATCTACAT CTTCCTCGGA AAGCTTGAAT 
TCATCAGCAC CACGTGGAGA ACGTTCGATC GCTGGGATTA GCTACGGTCA AGTGCGTGGC 
ACAGCTATTG AACAAAGGAC TTCCAGCGAC CACACAGACC ACACCTACCT GTCATCTACT 
TTCACCAAAG GAGAACGGGC GTTACTGTCC ATTACAGATA ACAGTTCATC CTCAGACATT 
GTGGAGAGCT CAACTTCTTA TATTAAAATC TCAAACTCTT CACATTCAGA GTATTCCTCC 
TTTTTTCATG CTCAGACTGA GAGAAGTAAC ATCTCATCCT ATGACGGGGA ATATGCTCAG 
CCTTCTACTG AGTCGCCAGT TCTGCATACA TCCAACCTTC CGTCCTACAC ACCCACCATT 
AATATGCCGA ACACTTCGGT TGTTCTGGAC ACTGATGCTG AGTTTGTTAG TGACTCCTCC 
TCCTCCTCTT CCTCCTCCTC CTCTTCTTCT TCTTCAGGGC CTCCTTTGCC TCTGCCCTCT 
GTGTCACAAT CCCACCATTT ATTTTCATCA ATTTTACCAT CAACCAGGGC CTCTGTGCAT 
CTACTAAAGT CTACCTCTGA TGCATCCACA CCATGGTCTT CCTCACCATC ACCTTTACCA 
GTATCCTTAA CGACATCTAC ATCTGCCCCA CTTTCTGTCT CACAAACAAC CTTGCCACAG 
TCATCTTCTA CCCCTGTCCT GCCCAGGGCA AGGGAGACTC CTGTGACTTC ATTTCAGACA 
TCAACAATGA CATCATTCAT GACAATGCTC CATAGTAGTC AAACTGCAGA CCTTAAGAGC 
CAGAGCACCC CACACCAAGA GAAAGTCATT ACAGAATCAA AGTCACCAAG CCTGGTGTCT 
CTGCCCACAG AGTCCACCAA AGCTGTAACA ACAAACTCTC CTTTGCCTCC ATCCTTAACA 
GAGTCCTCCA CAGAGCAAAC CCTTCCAGCC ACAAGCACCA ACTTAGCACA AATGTCTCCA 
ACTTTCACAA CTACCATTCT GAAGACCTCT CAGCCTCTTA TGACCACTCC TGGCACCCTG 
TCAAGCACAG CATCTCTGGT CACTGGCCCT ATAGCCGTAC AGACTACAGC TGGAAAACAG 
CTCTCGCTGA CCCATCCTGA AATACTAGTT CCTCAAATCT CAACAGAAGG TGGCATCAGC 
ACAGAAAGGA ACCGAGTGAT TGTGGATGCT ACCACTGGAT TGATCCCTTT GACCAGTGTA 
CCCACATCAG CAAAAGAAAT GACCACAAAG CTTGGCGTTA CAGCAGAGTA CAGCCCAGCT 
TCACGTTCCC TCGGAACATC TCCTTCTCCC CAAACCACAG TTGTTTCCAC GGCTGAAGAC 
TTGGCTCCCA AATCTGCCAC CTTTGCTGTT CAGAGCAGCA CACAGTCACC AACAACAGTG 
TCCTCTTCAG CCTCAGTCAA CAGCTGTGCT GTGAACCCTT GTCTTCACAA TGGCGAATGC 
GTCGCAGACA ACACCAGCCG TGGCTACCAC TGCAGGTGCC CGCCTTCCTG GCAAGGGGAT 
GATTGCAGTG TGGATGTGAA TGAGTGCCTG TCGAACCCCT GCCCATCCAC AGCCATGTGC 
AACAATACTC AGGGATCCTT TATCTGCAAA TGCCCGGTTG GGTACCAGTT GGAAAAAGGG 
ATATGCAATT TGGTTAGAAC CTTCGTGACA GAGTTTAAAT TAAAGAGAAC TTTTCTTAAT 
ACAACTGTGG AAAAACATTC AGACCTACAA GAAGTTGAAA ATGAGATCAC CAAAACGTTA 
AATATGTGTT TTTCAGCGTT ACCTAGTTAC ATCCGATCTA CAGTTCACGC CTCTAGGGAG 
TCCAACGCGG TGGTGATCTC ACTGCAAACA ACCTTTTCCC TGGCCTCCAA TGTGACGCTA 
TTTGACCTGG CTGATAGGAT GCAGAAATGT GTCAACTCCT GCAAGTCCTC TGCTGAGGTC 
TGCCAGCTCT TGGGATCTCA GAGGCGGATC TTTAGAGCGG GCAGCTTGTG CAAGCGGAAG 
AGTCCCGAAT GTGACAAAGA CACCTCCATC TGCACTGACC TGGACGGCGT TGCCCTGTGC 
CAGTGCAAGT CGGGATACTT TCAGTTCAAC AAGATGGACC ACTCCTGCCG AGCATGTGAA 
GATGGATATA GGCTTGAAAA TGAAACCTGC ATGAGTTGCC CATTTGGCCT TGGTGGTCTC 
AACTGTGGAA ACCCCTATCA GCTTATCACT GTGGTGATCG CAGCCGCGGG AGGTGGGCTC 
CTGCTCATCC TAGGCATCGC ACTGATTGTT ACCTGTTGCA GAAAGAATAA AAATGACATA 
AGCAAACTCA TCTTCAAAAG TGGAGATTTC CAAATGTCCC CGTATGCTGA ATACCCCAAA 
AATCCTCGCT CACAAGAATG GGGCCGAGAA GCTATTGAAA TGCATGAGAA TGGAAGTACC 
AAAAACCTCC TCCAGATGAC GGATGTGTAC TACTCGCCTA CAAGTGTAAG GAATCCAGAA 
CTTGAACGAA ACGGACTCTA CCCGGCCTAC ACTGGACTGC CAGGATCACG GCATTCTTGC 
ATTTTCCCCG GACAGTATAA CCCGTCTTTC ATCAGTGATG AAAGCAGAAG AAGAGACTAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEG1 ( NM_020733.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065784.1 Homo sapiens heart development protein with EGF like domains 1 (HEG1), mRNA.

Price & Availability↑

CloneID	OHu03459
Clone ID Related Accession (Same CDS sequence)	NM_020733.1 , NM_020733.2
Accession Version	NM_020733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	protein HEG homolog 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117488.11, DR003209.1, AB033063.2, AC092983.13 and AC026342.34. On Nov 23, 2018 this sequence version replaced NM_020733.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000311127.9/ ENSP00000311502.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCGC CGCGCGCCTC GCGGTGGCCG CCGCCGCTCC TGCTGCTGTT GCTGCCGCTG 
CTGCTGCTGC CGCCGGCGGC CCCCGGGACG CGGGACCCGC CGCCTTCCCC GGCTCGCCGC 
GCGCTGAGCC TGGCGCCCCT CGCGGGAGCG GGGCTGGAGC TGCAGCTGGA GCGCCGCCCG 
GAGCGCGAGC CGCCGCCCAC GCCGCCCCGG GAGCGCCGCG GGCCCGCGAC CCCCGGCCCC 
AGCTACAGGG CCCCTGAGCC AGGCGCCGCG ACACAGCGGG GACCCTCCGG CCGGGCCCCC 
AGAGGCGGGA GCGCGGATGC TGCCTGGAAA CATTGGCCAG AAAGTAACAC TGAGGCCCAT 
GTAGAAAACA TCACCTTCTA TCAGAATCAA GAGGACTTTT CAACAGTGTC CTCCAAAGAG 
GGCGTGATGG TTCAGACCTC TGGGAAGAGC CATGCTGCTT CGGATGCTCC AGAAAACCTC 
ACTCTACTCG CTGAAACAGC AGATGCTAGA GGAAGGAGCG GCTCTTCAAG TAGAACAAAC 
TTCACCATTT TGCCTGTTGG GTACTCACTG GAGATAGCAA CAGCTCTGAC TTCCCAGAGT 
GGCAACTTAG CCTCAGAAAG TCTTCACCTG CCATCCAGCA GTTCAGAGTT CGATGAAAGA 
ATTGCCGCTT TTCAAACAAA GAGTGGAACA GCCTCGGAGA TGGGAACAGA GAGGGCGATG 
GGGCTGTCAG AAGAATGGAC TGTGCACAGC CAAGAGGCCA CCACTTCGGC TTGGAGCCCG 
TCCTTTCTTC CTGCTTTGGA GATGGGAGAG CTGACCACGC CTTCTAGGAA GAGAAATTCC 
TCAGGACCAG ATCTCTCCTG GCTGCATTTC TACAGGACAG CAGCTTCCTC TCCTCTCTTA 
GACCTTTCCT CATCTTCTGA AAGTACAGAG AAGCTTAACA ACTCCACTGG CCTCCAGAGC 
TCCTCAGTCA GTCAAACAAA GACAATGCAT GTTGCCACCG TGTTCACTGA TGGTGGCCCG 
AGAACGCTGC GATCTTTGAC GGTCAGTCTG GGACCTGTGA GCAAGACAGA AGGCTTCCCC 
AAGGACTCCA GAATTGCCAC GACTTCATCC TCAGTCCTTC TTTCACCCTC TGCAGTGGAA 
TCGAGAAGAA ACAGTAGAGT AACTGGGAAT CCAGGGGATG AGGAATTCAT TGAACCATCC 
ACAGAAAATG AATTTGGACT TACGTCTTTG CGTTGGCAAA ATGATTCCCC AACCTTTGGA 
GAACATCAGC TTGCCAGCAG CTCTGAGGTG CAAAATGGAA GTCCCATGTC TCAGACTGAG 
ACTGTGTCTA GGTCAGTCGC ACCCATGAGA GGTGGAGAGA TCACTGCACA CTGGCTCTTG 
ACCAACAGCA CAACATCTGC AGATGTGACA GGAAGCTCTG CTTCATATCC TGAAGGTGTG 
AATGCTTCAG TGTTGACCCA GTTCTCAGAC TCTACTGTAC AGTCTGGAGG AAGTCACACA 
GCATTGGGAG ATAGGAGTTA TTCAGAGTCT TCATCTACAT CTTCCTCGGA AAGCTTGAAT 
TCATCAGCAC CACGTGGAGA ACGTTCGATC GCTGGGATTA GCTACGGTCA AGTGCGTGGC 
ACAGCTATTG AACAAAGGAC TTCCAGCGAC CACACAGACC ACACCTACCT GTCATCTACT 
TTCACCAAAG GAGAACGGGC GTTACTGTCC ATTACAGATA ACAGTTCATC CTCAGACATT 
GTGGAGAGCT CAACTTCTTA TATTAAAATC TCAAACTCTT CACATTCAGA GTATTCCTCC 
TTTTTTCATG CTCAGACTGA GAGAAGTAAC ATCTCATCCT ATGACGGGGA ATATGCTCAG 
CCTTCTACTG AGTCGCCAGT TCTGCATACA TCCAACCTTC CGTCCTACAC ACCCACCATT 
AATATGCCGA ACACTTCGGT TGTTCTGGAC ACTGATGCTG AGTTTGTTAG TGACTCCTCC 
TCCTCCTCTT CCTCCTCCTC CTCTTCTTCT TCTTCAGGGC CTCCTTTGCC TCTGCCCTCT 
GTGTCACAAT CCCACCATTT ATTTTCATCA ATTTTACCAT CAACCAGGGC CTCTGTGCAT 
CTACTAAAGT CTACCTCTGA TGCATCCACA CCATGGTCTT CCTCACCATC ACCTTTACCA 
GTATCCTTAA CGACATCTAC ATCTGCCCCA CTTTCTGTCT CACAAACAAC CTTGCCACAG 
TCATCTTCTA CCCCTGTCCT GCCCAGGGCA AGGGAGACTC CTGTGACTTC ATTTCAGACA 
TCAACAATGA CATCATTCAT GACAATGCTC CATAGTAGTC AAACTGCAGA CCTTAAGAGC 
CAGAGCACCC CACACCAAGA GAAAGTCATT ACAGAATCAA AGTCACCAAG CCTGGTGTCT 
CTGCCCACAG AGTCCACCAA AGCTGTAACA ACAAACTCTC CTTTGCCTCC ATCCTTAACA 
GAGTCCTCCA CAGAGCAAAC CCTTCCAGCC ACAAGCACCA ACTTAGCACA AATGTCTCCA 
ACTTTCACAA CTACCATTCT GAAGACCTCT CAGCCTCTTA TGACCACTCC TGGCACCCTG 
TCAAGCACAG CATCTCTGGT CACTGGCCCT ATAGCCGTAC AGACTACAGC TGGAAAACAG 
CTCTCGCTGA CCCATCCTGA AATACTAGTT CCTCAAATCT CAACAGAAGG TGGCATCAGC 
ACAGAAAGGA ACCGAGTGAT TGTGGATGCT ACCACTGGAT TGATCCCTTT GACCAGTGTA 
CCCACATCAG CAAAAGAAAT GACCACAAAG CTTGGCGTTA CAGCAGAGTA CAGCCCAGCT 
TCACGTTCCC TCGGAACATC TCCTTCTCCC CAAACCACAG TTGTTTCCAC GGCTGAAGAC 
TTGGCTCCCA AATCTGCCAC CTTTGCTGTT CAGAGCAGCA CACAGTCACC AACAACAGTG 
TCCTCTTCAG CCTCAGTCAA CAGCTGTGCT GTGAACCCTT GTCTTCACAA TGGCGAATGC 
GTCGCAGACA ACACCAGCCG TGGCTACCAC TGCAGGTGCC CGCCTTCCTG GCAAGGGGAT 
GATTGCAGTG TGGATGTGAA TGAGTGCCTG TCGAACCCCT GCCCATCCAC AGCCATGTGC 
AACAATACTC AGGGATCCTT TATCTGCAAA TGCCCGGTTG GGTACCAGTT GGAAAAAGGG 
ATATGCAATT TGGTTAGAAC CTTCGTGACA GAGTTTAAAT TAAAGAGAAC TTTTCTTAAT 
ACAACTGTGG AAAAACATTC AGACCTACAA GAAGTTGAAA ATGAGATCAC CAAAACGTTA 
AATATGTGTT TTTCAGCGTT ACCTAGTTAC ATCCGATCTA CAGTTCACGC CTCTAGGGAG 
TCCAACGCGG TGGTGATCTC ACTGCAAACA ACCTTTTCCC TGGCCTCCAA TGTGACGCTA 
TTTGACCTGG CTGATAGGAT GCAGAAATGT GTCAACTCCT GCAAGTCCTC TGCTGAGGTC 
TGCCAGCTCT TGGGATCTCA GAGGCGGATC TTTAGAGCGG GCAGCTTGTG CAAGCGGAAG 
AGTCCCGAAT GTGACAAAGA CACCTCCATC TGCACTGACC TGGACGGCGT TGCCCTGTGC 
CAGTGCAAGT CGGGATACTT TCAGTTCAAC AAGATGGACC ACTCCTGCCG AGCATGTGAA 
GATGGATATA GGCTTGAAAA TGAAACCTGC ATGAGTTGCC CATTTGGCCT TGGTGGTCTC 
AACTGTGGAA ACCCCTATCA GCTTATCACT GTGGTGATCG CAGCCGCGGG AGGTGGGCTC 
CTGCTCATCC TAGGCATCGC ACTGATTGTT ACCTGTTGCA GAAAGAATAA AAATGACATA 
AGCAAACTCA TCTTCAAAAG TGGAGATTTC CAAATGTCCC CGTATGCTGA ATACCCCAAA 
AATCCTCGCT CACAAGAATG GGGCCGAGAA GCTATTGAAA TGCATGAGAA TGGAAGTACC 
AAAAACCTCC TCCAGATGAC GGATGTGTAC TACTCGCCTA CAAGTGTAAG GAATCCAGAA 
CTTGAACGAA ACGGACTCTA CCCGGCCTAC ACTGGACTGC CAGGATCACG GCATTCTTGC 
ATTTTCCCCG GACAGTATAA CCCGTCTTTC ATCAGTGATG AAAGCAGAAG AAGAGACTAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065784.1
CDS	108..4253
Translation	MASPRASRWPPPLLLLLLPLLLLPPAAPGTRDPPPSPARRALSLAPLAGAGLELQLERRPEREPPPTPPRERRGPATPGPSYRAPEPGAATQRGPSGRAPRGGSADAAWKHWPESNTEAHVENITFYQNQEDFSTVSSKEGVMVQTSGKSHAASDAPENLTLLAETADARGRSGSSSRTNFTILPVGYSLEIATALTSQSGNLASESLHLPSSSSEFDERIAAFQTKSGTASEMGTERAMGLSEEWTVHSQEATTSAWSPSFLPALEMGELTTPSRKRNSSGPDLSWLHFYRTAASSPLLDLSSSSESTEKLNNSTGLQSSSVSQTKTMHVATVFTDGGPRTLRSLTVSLGPVSKTEGFPKDSRIATTSSSVLLSPSAVESRRNSRVTGNPGDEEFIEPSTENEFGLTSLRWQNDSPTFGEHQLASSSEVQNGSPMSQTETVSRSVAPMRGGEITAHWLLTNSTTSADVTGSSASYPEGVNASVLTQFSDSTVQSGGSHTALGDRSYSESSSTSSSESLNSSAPRGERSIAGISYGQVRGTAIEQRTSSDHTDHTYLSSTFTKGERALLSITDNSSSSDIVESSTSYIKISNSSHSEYSSFFHAQTERSNISSYDGEYAQPSTESPVLHTSNLPSYTPTINMPNTSVVLDTDAEFVSDSSSSSSSSSSSSSSGPPLPLPSVSQSHHLFSSILPSTRASVHLLKSTSDASTPWSSSPSPLPVSLTTSTSAPLSVSQTTLPQSSSTPVLPRARETPVTSFQTSTMTSFMTMLHSSQTADLKSQSTPHQEKVITESKSPSLVSLPTESTKAVTTNSPLPPSLTESSTEQTLPATSTNLAQMSPTFTTTILKTSQPLMTTPGTLSSTASLVTGPIAVQTTAGKQLSLTHPEILVPQISTEGGISTERNRVIVDATTGLIPLTSVPTSAKEMTTKLGVTAEYSPASRSLGTSPSPQTTVVSTAEDLAPKSATFAVQSSTQSPTTVSSSASVNSCAVNPCLHNGECVADNTSRGYHCRCPPSWQGDDCSVDVNECLSNPCPSTAMCNNTQGSFICKCPVGYQLEKGICNLVRTFVTEFKLKRTFLNTTVEKHSDLQEVENEITKTLNMCFSALPSYIRSTVHASRESNAVVISLQTTFSLASNVTLFDLADRMQKCVNSCKSSAEVCQLLGSQRRIFRAGSLCKRKSPECDKDTSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGIALIVTCCRKNKNDISKLIFKSGDFQMSPYAEYPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTSVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	NM_020733.2
Organism	Homo sapiens(human)
Definition	Homo sapiens heart development protein with EGF like domains 1 (HEG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020733.2

ORF Insert Sequence:

ATGGCCTCGC CGCGCGCCTC GCGGTGGCCG CCGCCGCTCC TGCTGCTGTT GCTGCCGCTG 
CTGCTGCTGC CGCCGGCGGC CCCCGGGACG CGGGACCCGC CGCCTTCCCC GGCTCGCCGC 
GCGCTGAGCC TGGCGCCCCT CGCGGGAGCG GGGCTGGAGC TGCAGCTGGA GCGCCGCCCG 
GAGCGCGAGC CGCCGCCCAC GCCGCCCCGG GAGCGCCGCG GGCCCGCGAC CCCCGGCCCC 
AGCTACAGGG CCCCTGAGCC AGGCGCCGCG ACACAGCGGG GACCCTCCGG CCGGGCCCCC 
AGAGGCGGGA GCGCGGATGC TGCCTGGAAA CATTGGCCAG AAAGTAACAC TGAGGCCCAT 
GTAGAAAACA TCACCTTCTA TCAGAATCAA GAGGACTTTT CAACAGTGTC CTCCAAAGAG 
GGCGTGATGG TTCAGACCTC TGGGAAGAGC CATGCTGCTT CGGATGCTCC AGAAAACCTC 
ACTCTACTCG CTGAAACAGC AGATGCTAGA GGAAGGAGCG GCTCTTCAAG TAGAACAAAC 
TTCACCATTT TGCCTGTTGG GTACTCACTG GAGATAGCAA CAGCTCTGAC TTCCCAGAGT 
GGCAACTTAG CCTCAGAAAG TCTTCACCTG CCATCCAGCA GTTCAGAGTT CGATGAAAGA 
ATTGCCGCTT TTCAAACAAA GAGTGGAACA GCCTCGGAGA TGGGAACAGA GAGGGCGATG 
GGGCTGTCAG AAGAATGGAC TGTGCACAGC CAAGAGGCCA CCACTTCGGC TTGGAGCCCG 
TCCTTTCTTC CTGCTTTGGA GATGGGAGAG CTGACCACGC CTTCTAGGAA GAGAAATTCC 
TCAGGACCAG ATCTCTCCTG GCTGCATTTC TACAGGACAG CAGCTTCCTC TCCTCTCTTA 
GACCTTTCCT CATCTTCTGA AAGTACAGAG AAGCTTAACA ACTCCACTGG CCTCCAGAGC 
TCCTCAGTCA GTCAAACAAA GACAATGCAT GTTGCCACCG TGTTCACTGA TGGTGGCCCG 
AGAACGCTGC GATCTTTGAC GGTCAGTCTG GGACCTGTGA GCAAGACAGA AGGCTTCCCC 
AAGGACTCCA GAATTGCCAC GACTTCATCC TCAGTCCTTC TTTCACCCTC TGCAGTGGAA 
TCGAGAAGAA ACAGTAGAGT AACTGGGAAT CCAGGGGATG AGGAATTCAT TGAACCATCC 
ACAGAAAATG AATTTGGACT TACGTCTTTG CGTTGGCAAA ATGATTCCCC AACCTTTGGA 
GAACATCAGC TTGCCAGCAG CTCTGAGGTG CAAAATGGAA GTCCCATGTC TCAGACTGAG 
ACTGTGTCTA GGTCAGTCGC ACCCATGAGA GGTGGAGAGA TCACTGCACA CTGGCTCTTG 
ACCAACAGCA CAACATCTGC AGATGTGACA GGAAGCTCTG CTTCATATCC TGAAGGTGTG 
AATGCTTCAG TGTTGACCCA GTTCTCAGAC TCTACTGTAC AGTCTGGAGG AAGTCACACA 
GCATTGGGAG ATAGGAGTTA TTCAGAGTCT TCATCTACAT CTTCCTCGGA AAGCTTGAAT 
TCATCAGCAC CACGTGGAGA ACGTTCGATC GCTGGGATTA GCTACGGTCA AGTGCGTGGC 
ACAGCTATTG AACAAAGGAC TTCCAGCGAC CACACAGACC ACACCTACCT GTCATCTACT 
TTCACCAAAG GAGAACGGGC GTTACTGTCC ATTACAGATA ACAGTTCATC CTCAGACATT 
GTGGAGAGCT CAACTTCTTA TATTAAAATC TCAAACTCTT CACATTCAGA GTATTCCTCC 
TTTTTTCATG CTCAGACTGA GAGAAGTAAC ATCTCATCCT ATGACGGGGA ATATGCTCAG 
CCTTCTACTG AGTCGCCAGT TCTGCATACA TCCAACCTTC CGTCCTACAC ACCCACCATT 
AATATGCCGA ACACTTCGGT TGTTCTGGAC ACTGATGCTG AGTTTGTTAG TGACTCCTCC 
TCCTCCTCTT CCTCCTCCTC CTCTTCTTCT TCTTCAGGGC CTCCTTTGCC TCTGCCCTCT 
GTGTCACAAT CCCACCATTT ATTTTCATCA ATTTTACCAT CAACCAGGGC CTCTGTGCAT 
CTACTAAAGT CTACCTCTGA TGCATCCACA CCATGGTCTT CCTCACCATC ACCTTTACCA 
GTATCCTTAA CGACATCTAC ATCTGCCCCA CTTTCTGTCT CACAAACAAC CTTGCCACAG 
TCATCTTCTA CCCCTGTCCT GCCCAGGGCA AGGGAGACTC CTGTGACTTC ATTTCAGACA 
TCAACAATGA CATCATTCAT GACAATGCTC CATAGTAGTC AAACTGCAGA CCTTAAGAGC 
CAGAGCACCC CACACCAAGA GAAAGTCATT ACAGAATCAA AGTCACCAAG CCTGGTGTCT 
CTGCCCACAG AGTCCACCAA AGCTGTAACA ACAAACTCTC CTTTGCCTCC ATCCTTAACA 
GAGTCCTCCA CAGAGCAAAC CCTTCCAGCC ACAAGCACCA ACTTAGCACA AATGTCTCCA 
ACTTTCACAA CTACCATTCT GAAGACCTCT CAGCCTCTTA TGACCACTCC TGGCACCCTG 
TCAAGCACAG CATCTCTGGT CACTGGCCCT ATAGCCGTAC AGACTACAGC TGGAAAACAG 
CTCTCGCTGA CCCATCCTGA AATACTAGTT CCTCAAATCT CAACAGAAGG TGGCATCAGC 
ACAGAAAGGA ACCGAGTGAT TGTGGATGCT ACCACTGGAT TGATCCCTTT GACCAGTGTA 
CCCACATCAG CAAAAGAAAT GACCACAAAG CTTGGCGTTA CAGCAGAGTA CAGCCCAGCT 
TCACGTTCCC TCGGAACATC TCCTTCTCCC CAAACCACAG TTGTTTCCAC GGCTGAAGAC 
TTGGCTCCCA AATCTGCCAC CTTTGCTGTT CAGAGCAGCA CACAGTCACC AACAACAGTG 
TCCTCTTCAG CCTCAGTCAA CAGCTGTGCT GTGAACCCTT GTCTTCACAA TGGCGAATGC 
GTCGCAGACA ACACCAGCCG TGGCTACCAC TGCAGGTGCC CGCCTTCCTG GCAAGGGGAT 
GATTGCAGTG TGGATGTGAA TGAGTGCCTG TCGAACCCCT GCCCATCCAC AGCCATGTGC 
AACAATACTC AGGGATCCTT TATCTGCAAA TGCCCGGTTG GGTACCAGTT GGAAAAAGGG 
ATATGCAATT TGGTTAGAAC CTTCGTGACA GAGTTTAAAT TAAAGAGAAC TTTTCTTAAT 
ACAACTGTGG AAAAACATTC AGACCTACAA GAAGTTGAAA ATGAGATCAC CAAAACGTTA 
AATATGTGTT TTTCAGCGTT ACCTAGTTAC ATCCGATCTA CAGTTCACGC CTCTAGGGAG 
TCCAACGCGG TGGTGATCTC ACTGCAAACA ACCTTTTCCC TGGCCTCCAA TGTGACGCTA 
TTTGACCTGG CTGATAGGAT GCAGAAATGT GTCAACTCCT GCAAGTCCTC TGCTGAGGTC 
TGCCAGCTCT TGGGATCTCA GAGGCGGATC TTTAGAGCGG GCAGCTTGTG CAAGCGGAAG 
AGTCCCGAAT GTGACAAAGA CACCTCCATC TGCACTGACC TGGACGGCGT TGCCCTGTGC 
CAGTGCAAGT CGGGATACTT TCAGTTCAAC AAGATGGACC ACTCCTGCCG AGCATGTGAA 
GATGGATATA GGCTTGAAAA TGAAACCTGC ATGAGTTGCC CATTTGGCCT TGGTGGTCTC 
AACTGTGGAA ACCCCTATCA GCTTATCACT GTGGTGATCG CAGCCGCGGG AGGTGGGCTC 
CTGCTCATCC TAGGCATCGC ACTGATTGTT ACCTGTTGCA GAAAGAATAA AAATGACATA 
AGCAAACTCA TCTTCAAAAG TGGAGATTTC CAAATGTCCC CGTATGCTGA ATACCCCAAA 
AATCCTCGCT CACAAGAATG GGGCCGAGAA GCTATTGAAA TGCATGAGAA TGGAAGTACC 
AAAAACCTCC TCCAGATGAC GGATGTGTAC TACTCGCCTA CAAGTGTAAG GAATCCAGAA 
CTTGAACGAA ACGGACTCTA CCCGGCCTAC ACTGGACTGC CAGGATCACG GCATTCTTGC 
ATTTTCCCCG GACAGTATAA CCCGTCTTTC ATCAGTGATG AAAGCAGAAG AAGAGACTAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEG1 ( XM_005247666.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247723.1 Homo sapiens heart development protein with EGF like domains 1 (HEG1), transcript variant X1, mRNA.

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CloneID	OHu38076
Clone ID Related Accession (Same CDS sequence)	XM_005247666.1
Accession Version	XM_005247666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein HEG homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGC CGCGCGCCTC GCGGTGGCCG CCGCCGCTCC TGCTGCTGTT GCTGCCGCTG 
CTGCTGCTGC CGCCGGCGGC CCCCGGGACG CGGGACCCGC CGCCTTCCCC GGCTCGCCGC 
GCGCTGAGCC TGGCGCCCCT CGCGGGAGCG GGGCTGGAGC TGCAGCTGGA GCGCCGCCCG 
GAGCGCGAGC CGCCGCCCAC GCCGCCCCGG GAGCGCCGCG GGCCCGCGAC CCCCGGCCCC 
AGCTACAGGG CCCCTGAGCC AGGCGCCGCG ACACAGCGGG GACCCTCCGG CCGGGCCCCC 
AGAGGCGGGA GCGCGGATGC TGCCTGGAAA CATTGGCCAG AAAGTAACAC TGAGGCCCAT 
GTAGAAAACA TCACCTTCTA TCAGAATCAA GAGGACTTTT CAACAGTGTC CTCCAAAGAG 
GGCGTGATGG TTCAGACCTC TGGGAAGAGC CATGCTGCTT CGGATGCTCC AGAAAACCTC 
ACTCTACTCG CTGAAACAGC AGATGCTAGA GGAAGGAGCG GCTCTTCAAG TAGAACAAAC 
TTCACCATTT TGCCTGTTGG GTACTCACTG GAGATAGCAA CAGCTCTGAC TTCCCAGAGT 
GGCAACTTAG CCTCAGAAAG TCTTCACCTG CCATCCAGCA GTTCAGAGTT CGATGAAAGA 
ATTGCCGCTT TTCAAACAAA GAGTGGAACA GCCTCGGAGA TGGGAACAGA GAGGGCGATG 
GGGCTGTCAG AAGAATGGAC TGTGCACAGC CAAGAGGCCA CCACTTCGGC TTGGAGCCCG 
TCCTTTCTTC CTGCTTTGGA GATGGGAGAG CTGACCACGC CTTCTAGGAA GAGAAATTCC 
TCAGGACCAG ATCTCTCCTG GCTGCATTTC TACAGGACAG CAGCTTCCTC TCCTCTCTTA 
GACCTTTCCT CATCTTCTGA AAGTACAGAG AAGCTTAACA ACTCCACTGG CCTCCAGAGC 
TCCTCAGTCA GTCAAACAAA GACAATGCAT GTTGCCACCG TGTTCACTGA TGGTGGCCCG 
AGAACGCTGC GATCTTTGAC GGTCAGTCTG GGACCTGTGA GCAAGACAGA AGGCTTCCCC 
AAGGACTCCA GAATTGCCAC GACTTCATCC TCAGTCCTTC TTTCACCCTC TGCAGTGGAA 
TCGAGAAGAA ACAGTAGAGT AACTGGGAAT CCAGGGGATG AGGAATTCAT TGAACCATCC 
ACAGAAAATG AATTTGGACT TACGTCTTTG CGTTGGCAAA ATGATTCCCC AACCTTTGGA 
GAACATCAGC TTGCCAGCAG CTCTGAGGTG CAAAATGGAA GTCCCATGTC TCAGACTGAG 
ACTGTGTCTA GGTCAGTCGC ACCCATGAGA GGTGGAGAGA TCACTGCACA CTGGCTCTTG 
ACCAACAGCA CAACATCTGC AGATGTGACA GGAAGCTCTG CTTCATATCC TGAAGGTGTG 
AATGCTTCAG TGTTGACCCA GTTCTCAGAC TCTACTGTAC AGTCTGGAGG AAGTCACACA 
GCATTGGGAG ATAGGAGTTA TTCAGAGTCT TCATCTACAT CTTCCTCGGA AAGCTTGAAT 
TCATCAGCAC CACGTGGAGA ACGTTCGACC TTGGAAGACA GCCGAGAGCC AGGCCAAGCA 
CTAGGTGACA GTTCCGCCAA TGCAGAGGAC AGGACTTCTG GGGTGCCCTC TCTCGGCACC 
CACACCTTGG CTACTGTCAC TGGAAACGGG GAACGCACAC TGCGGTCTGT CACCCTCACC 
AACACCAGCA TGAGCACGAC TTCTGGGGAA GCAGGCAGCC CTGCAGCGGC CATGCACCAA 
GAAACAGAGG GTGCCTCTCT GCACGTAAAC GTGACGGACG ACATGGGCCT GGTCTCACGG 
TCACTGGCCG CCTCCAGTGC ACTCGGAGTC GCTGGGATTA GCTACGGTCA AGTGCGTGGC 
ACAGCTATTG AACAAAGGAC TTCCAGCGAC CACACAGACC ACACCTACCT GTCATCTACT 
TTCACCAAAG GAGAACGGGC GTTACTGTCC ATTACAGATA ACAGTTCATC CTCAGACATT 
GTGGAGAGCT CAACTTCTTA TATTAAAATC TCAAACTCTT CACATTCAGA GTATTCCTCC 
TTTTTTCATG CTCAGACTGA GAGAAGTAAC ATCTCATCCT ATGACGGGGA ATATGCTCAG 
CCTTCTACTG AGTCGCCAGT TCTGCATACA TCCAACCTTC CGTCCTACAC ACCCACCATT 
AATATGCCGA ACACTTCGGT TGTTCTGGAC ACTGATGCTG AGTTTGTTAG TGACTCCTCC 
TCCTCCTCTT CCTCCTCCTC CTCTTCTTCT TCTTCAGGGC CTCCTTTGCC TCTGCCCTCT 
GTGTCACAAT CCCACCATTT ATTTTCATCA ATTTTACCAT CAACCAGGGC CTCTGTGCAT 
CTACTAAAGT CTACCTCTGA TGCATCCACA CCATGGTCTT CCTCACCATC ACCTTTACCA 
GTATCCTTAA CGACATCTAC ATCTGCCCCA CTTTCTGTCT CACAAACAAC CTTGCCACAG 
TCATCTTCTA CCCCTGTCCT GCCCAGGGCA AGGGAGACTC CTGTGACTTC ATTTCAGACA 
TCAACAATGA CATCATTCAT GACAATGCTC CATAGTAGTC AAACTGCAGA CCTTAAGAGC 
CAGAGCACCC CACACCAAGA GAAAGTCATT ACAGAATCAA AGTCACCAAG CCTGGTGTCT 
CTGCCCACAG AGTCCACCAA AGCTGTAACA ACAAACTCTC CTTTGCCTCC ATCCTTAACA 
GAGTCCTCCA CAGAGCAAAC CCTTCCAGCC ACAAGCACCA ACTTAGCACA AATGTCTCCA 
ACTTTCACAA CTACCATTCT GAAGACCTCT CAGCCTCTTA TGACCACTCC TGGCACCCTG 
TCAAGCACAG CATCTCTGGT CACTGGCCCT ATAGCCGTAC AGACTACAGC TGGAAAACAG 
CTCTCGCTGA CCCATCCTGA AATACTAGTT CCTCAAATCT CAACAGAAGG TGGCATCAGC 
ACAGAAAGGA ACCGAGTGAT TGTGGATGCT ACCACTGGAT TGATCCCTTT GACCAGTGTA 
CCCACATCAG CAAAAGAAAT GACCACAAAG CTTGGCGTTA CAGCAGAGTA CAGCCCAGCT 
TCACGTTCCC TCGGAACATC TCCTTCTCCC CAAACCACAG TTGTTTCCAC GGCTGAAGAC 
TTGGCTCCCA AATCTGCCAC CTTTGCTGTT CAGAGCAGCA CACAGTCACC AACAACAGTG 
TCCTCTTCAG CCTCAGTCAA CAGCTGTGCT GTGAACCCTT GTCTTCACAA TGGCGAATGC 
GTCGCAGACA ACACCAGCCG TGGCTACCAC TGCAGGTGCC CGCCTTCCTG GCAAGGGGAT 
GATTGCAGTG TGGATGTGAA TGAGTGCCTG TCGAACCCCT GCCCATCCAC AGCCATGTGC 
AACAATACTC AGGGATCCTT TATCTGCAAA TGCCCGGTTG GGTACCAGTT GGAAAAAGGG 
ATATGCAATT TGGTTAGAAC CTTCGTGACA GAGTTTAAAT TAAAGAGAAC TTTTCTTAAT 
ACAACTGTGG AAAAACATTC AGACCTACAA GAAGTTGAAA ATGAGATCAC CAAAACGTTA 
AATATGTGTT TTTCAGCGTT ACCTAGTTAC ATCCGATCTA CAGTTCACGC CTCTAGGGAG 
TCCAACGCGG TGGTGATCTC ACTGCAAACA ACCTTTTCCC TGGCCTCCAA TGTGACGCTA 
TTTGACCTGG CTGATAGGAT GCAGAAATGT GTCAACTCCT GCAAGTCCTC TGCTGAGGTC 
TGCCAGCTCT TGGGATCTCA GAGGCGGATC TTTAGAGCGG GCAGCTTGTG CAAGCGGAAG 
AGTCCCGAAT GTGACAAAGA CACCTCCATC TGCACTGACC TGGACGGCGT TGCCCTGTGC 
CAGTGCAAGT CGGGATACTT TCAGTTCAAC AAGATGGACC ACTCCTGCCG AGCATGTGAA 
GATGGATATA GGCTTGAAAA TGAAACCTGC ATGAGTTGCC CATTTGGCCT TGGTGGTCTC 
AACTGTGGAA ACCCCTATCA GCTTATCACT GTGGTGATCG CAGCCGCGGG AGGTGGGCTC 
CTGCTCATCC TAGGCATCGC ACTGATTGTT ACCTGTTGCA GAAAGAATAA AAATGACATA 
AGCAAACTCA TCTTCAAAAG TGGAGATTTC CAAATGTCCC CGTATGCTGA ATACCCCAAA 
AATCCTCGCT CACAAGAATG GGGCCGAGAA GCTATTGAAA TGCATGAGAA TGGAAGTACC 
AAAAACCTCC TCCAGATGAC GGATGTGTAC TACTCGCCTA CAAGTGTAAG GAATCCAGAA 
CTTGAACGAA ACGGACTCTA CCCGGCCTAC ACTGGACTGC CAGGATCACG GCATTCTTGC 
ATTTTCCCCG GACAGTATAA CCCGTCTTTC ATCAGTGATG AAAGCAGAAG AAGAGACTAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247723.1
CDS	64..4509
Translation	MASPRASRWPPPLLLLLLPLLLLPPAAPGTRDPPPSPARRALSLAPLAGAGLELQLERRPEREPPPTPPRERRGPATPGPSYRAPEPGAATQRGPSGRAPRGGSADAAWKHWPESNTEAHVENITFYQNQEDFSTVSSKEGVMVQTSGKSHAASDAPENLTLLAETADARGRSGSSSRTNFTILPVGYSLEIATALTSQSGNLASESLHLPSSSSEFDERIAAFQTKSGTASEMGTERAMGLSEEWTVHSQEATTSAWSPSFLPALEMGELTTPSRKRNSSGPDLSWLHFYRTAASSPLLDLSSSSESTEKLNNSTGLQSSSVSQTKTMHVATVFTDGGPRTLRSLTVSLGPVSKTEGFPKDSRIATTSSSVLLSPSAVESRRNSRVTGNPGDEEFIEPSTENEFGLTSLRWQNDSPTFGEHQLASSSEVQNGSPMSQTETVSRSVAPMRGGEITAHWLLTNSTTSADVTGSSASYPEGVNASVLTQFSDSTVQSGGSHTALGDRSYSESSSTSSSESLNSSAPRGERSTLEDSREPGQALGDSSANAEDRTSGVPSLGTHTLATVTGNGERTLRSVTLTNTSMSTTSGEAGSPAAAMHQETEGASLHVNVTDDMGLVSRSLAASSALGVAGISYGQVRGTAIEQRTSSDHTDHTYLSSTFTKGERALLSITDNSSSSDIVESSTSYIKISNSSHSEYSSFFHAQTERSNISSYDGEYAQPSTESPVLHTSNLPSYTPTINMPNTSVVLDTDAEFVSDSSSSSSSSSSSSSSGPPLPLPSVSQSHHLFSSILPSTRASVHLLKSTSDASTPWSSSPSPLPVSLTTSTSAPLSVSQTTLPQSSSTPVLPRARETPVTSFQTSTMTSFMTMLHSSQTADLKSQSTPHQEKVITESKSPSLVSLPTESTKAVTTNSPLPPSLTESSTEQTLPATSTNLAQMSPTFTTTILKTSQPLMTTPGTLSSTASLVTGPIAVQTTAGKQLSLTHPEILVPQISTEGGISTERNRVIVDATTGLIPLTSVPTSAKEMTTKLGVTAEYSPASRSLGTSPSPQTTVVSTAEDLAPKSATFAVQSSTQSPTTVSSSASVNSCAVNPCLHNGECVADNTSRGYHCRCPPSWQGDDCSVDVNECLSNPCPSTAMCNNTQGSFICKCPVGYQLEKGICNLVRTFVTEFKLKRTFLNTTVEKHSDLQEVENEITKTLNMCFSALPSYIRSTVHASRESNAVVISLQTTFSLASNVTLFDLADRMQKCVNSCKSSAEVCQLLGSQRRIFRAGSLCKRKSPECDKDTSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGIALIVTCCRKNKNDISKLIFKSGDFQMSPYAEYPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTSVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

Target ORF information:

RefSeq Version	XM_005247666.1
Organism	Homo sapiens(human)
Definition	Homo sapiens heart development protein with EGF like domains 1 (HEG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247666.1

ORF Insert Sequence:

ATGGCCTCGC CGCGCGCCTC GCGGTGGCCG CCGCCGCTCC TGCTGCTGTT GCTGCCGCTG 
CTGCTGCTGC CGCCGGCGGC CCCCGGGACG CGGGACCCGC CGCCTTCCCC GGCTCGCCGC 
GCGCTGAGCC TGGCGCCCCT CGCGGGAGCG GGGCTGGAGC TGCAGCTGGA GCGCCGCCCG 
GAGCGCGAGC CGCCGCCCAC GCCGCCCCGG GAGCGCCGCG GGCCCGCGAC CCCCGGCCCC 
AGCTACAGGG CCCCTGAGCC AGGCGCCGCG ACACAGCGGG GACCCTCCGG CCGGGCCCCC 
AGAGGCGGGA GCGCGGATGC TGCCTGGAAA CATTGGCCAG AAAGTAACAC TGAGGCCCAT 
GTAGAAAACA TCACCTTCTA TCAGAATCAA GAGGACTTTT CAACAGTGTC CTCCAAAGAG 
GGCGTGATGG TTCAGACCTC TGGGAAGAGC CATGCTGCTT CGGATGCTCC AGAAAACCTC 
ACTCTACTCG CTGAAACAGC AGATGCTAGA GGAAGGAGCG GCTCTTCAAG TAGAACAAAC 
TTCACCATTT TGCCTGTTGG GTACTCACTG GAGATAGCAA CAGCTCTGAC TTCCCAGAGT 
GGCAACTTAG CCTCAGAAAG TCTTCACCTG CCATCCAGCA GTTCAGAGTT CGATGAAAGA 
ATTGCCGCTT TTCAAACAAA GAGTGGAACA GCCTCGGAGA TGGGAACAGA GAGGGCGATG 
GGGCTGTCAG AAGAATGGAC TGTGCACAGC CAAGAGGCCA CCACTTCGGC TTGGAGCCCG 
TCCTTTCTTC CTGCTTTGGA GATGGGAGAG CTGACCACGC CTTCTAGGAA GAGAAATTCC 
TCAGGACCAG ATCTCTCCTG GCTGCATTTC TACAGGACAG CAGCTTCCTC TCCTCTCTTA 
GACCTTTCCT CATCTTCTGA AAGTACAGAG AAGCTTAACA ACTCCACTGG CCTCCAGAGC 
TCCTCAGTCA GTCAAACAAA GACAATGCAT GTTGCCACCG TGTTCACTGA TGGTGGCCCG 
AGAACGCTGC GATCTTTGAC GGTCAGTCTG GGACCTGTGA GCAAGACAGA AGGCTTCCCC 
AAGGACTCCA GAATTGCCAC GACTTCATCC TCAGTCCTTC TTTCACCCTC TGCAGTGGAA 
TCGAGAAGAA ACAGTAGAGT AACTGGGAAT CCAGGGGATG AGGAATTCAT TGAACCATCC 
ACAGAAAATG AATTTGGACT TACGTCTTTG CGTTGGCAAA ATGATTCCCC AACCTTTGGA 
GAACATCAGC TTGCCAGCAG CTCTGAGGTG CAAAATGGAA GTCCCATGTC TCAGACTGAG 
ACTGTGTCTA GGTCAGTCGC ACCCATGAGA GGTGGAGAGA TCACTGCACA CTGGCTCTTG 
ACCAACAGCA CAACATCTGC AGATGTGACA GGAAGCTCTG CTTCATATCC TGAAGGTGTG 
AATGCTTCAG TGTTGACCCA GTTCTCAGAC TCTACTGTAC AGTCTGGAGG AAGTCACACA 
GCATTGGGAG ATAGGAGTTA TTCAGAGTCT TCATCTACAT CTTCCTCGGA AAGCTTGAAT 
TCATCAGCAC CACGTGGAGA ACGTTCGACC TTGGAAGACA GCCGAGAGCC AGGCCAAGCA 
CTAGGTGACA GTTCCGCCAA TGCAGAGGAC AGGACTTCTG GGGTGCCCTC TCTCGGCACC 
CACACCTTGG CTACTGTCAC TGGAAACGGG GAACGCACAC TGCGGTCTGT CACCCTCACC 
AACACCAGCA TGAGCACGAC TTCTGGGGAA GCAGGCAGCC CTGCAGCGGC CATGCACCAA 
GAAACAGAGG GTGCCTCTCT GCACGTAAAC GTGACGGACG ACATGGGCCT GGTCTCACGG 
TCACTGGCCG CCTCCAGTGC ACTCGGAGTC GCTGGGATTA GCTACGGTCA AGTGCGTGGC 
ACAGCTATTG AACAAAGGAC TTCCAGCGAC CACACAGACC ACACCTACCT GTCATCTACT 
TTCACCAAAG GAGAACGGGC GTTACTGTCC ATTACAGATA ACAGTTCATC CTCAGACATT 
GTGGAGAGCT CAACTTCTTA TATTAAAATC TCAAACTCTT CACATTCAGA GTATTCCTCC 
TTTTTTCATG CTCAGACTGA GAGAAGTAAC ATCTCATCCT ATGACGGGGA ATATGCTCAG 
CCTTCTACTG AGTCGCCAGT TCTGCATACA TCCAACCTTC CGTCCTACAC ACCCACCATT 
AATATGCCGA ACACTTCGGT TGTTCTGGAC ACTGATGCTG AGTTTGTTAG TGACTCCTCC 
TCCTCCTCTT CCTCCTCCTC CTCTTCTTCT TCTTCAGGGC CTCCTTTGCC TCTGCCCTCT 
GTGTCACAAT CCCACCATTT ATTTTCATCA ATTTTACCAT CAACCAGGGC CTCTGTGCAT 
CTACTAAAGT CTACCTCTGA TGCATCCACA CCATGGTCTT CCTCACCATC ACCTTTACCA 
GTATCCTTAA CGACATCTAC ATCTGCCCCA CTTTCTGTCT CACAAACAAC CTTGCCACAG 
TCATCTTCTA CCCCTGTCCT GCCCAGGGCA AGGGAGACTC CTGTGACTTC ATTTCAGACA 
TCAACAATGA CATCATTCAT GACAATGCTC CATAGTAGTC AAACTGCAGA CCTTAAGAGC 
CAGAGCACCC CACACCAAGA GAAAGTCATT ACAGAATCAA AGTCACCAAG CCTGGTGTCT 
CTGCCCACAG AGTCCACCAA AGCTGTAACA ACAAACTCTC CTTTGCCTCC ATCCTTAACA 
GAGTCCTCCA CAGAGCAAAC CCTTCCAGCC ACAAGCACCA ACTTAGCACA AATGTCTCCA 
ACTTTCACAA CTACCATTCT GAAGACCTCT CAGCCTCTTA TGACCACTCC TGGCACCCTG 
TCAAGCACAG CATCTCTGGT CACTGGCCCT ATAGCCGTAC AGACTACAGC TGGAAAACAG 
CTCTCGCTGA CCCATCCTGA AATACTAGTT CCTCAAATCT CAACAGAAGG TGGCATCAGC 
ACAGAAAGGA ACCGAGTGAT TGTGGATGCT ACCACTGGAT TGATCCCTTT GACCAGTGTA 
CCCACATCAG CAAAAGAAAT GACCACAAAG CTTGGCGTTA CAGCAGAGTA CAGCCCAGCT 
TCACGTTCCC TCGGAACATC TCCTTCTCCC CAAACCACAG TTGTTTCCAC GGCTGAAGAC 
TTGGCTCCCA AATCTGCCAC CTTTGCTGTT CAGAGCAGCA CACAGTCACC AACAACAGTG 
TCCTCTTCAG CCTCAGTCAA CAGCTGTGCT GTGAACCCTT GTCTTCACAA TGGCGAATGC 
GTCGCAGACA ACACCAGCCG TGGCTACCAC TGCAGGTGCC CGCCTTCCTG GCAAGGGGAT 
GATTGCAGTG TGGATGTGAA TGAGTGCCTG TCGAACCCCT GCCCATCCAC AGCCATGTGC 
AACAATACTC AGGGATCCTT TATCTGCAAA TGCCCGGTTG GGTACCAGTT GGAAAAAGGG 
ATATGCAATT TGGTTAGAAC CTTCGTGACA GAGTTTAAAT TAAAGAGAAC TTTTCTTAAT 
ACAACTGTGG AAAAACATTC AGACCTACAA GAAGTTGAAA ATGAGATCAC CAAAACGTTA 
AATATGTGTT TTTCAGCGTT ACCTAGTTAC ATCCGATCTA CAGTTCACGC CTCTAGGGAG 
TCCAACGCGG TGGTGATCTC ACTGCAAACA ACCTTTTCCC TGGCCTCCAA TGTGACGCTA 
TTTGACCTGG CTGATAGGAT GCAGAAATGT GTCAACTCCT GCAAGTCCTC TGCTGAGGTC 
TGCCAGCTCT TGGGATCTCA GAGGCGGATC TTTAGAGCGG GCAGCTTGTG CAAGCGGAAG 
AGTCCCGAAT GTGACAAAGA CACCTCCATC TGCACTGACC TGGACGGCGT TGCCCTGTGC 
CAGTGCAAGT CGGGATACTT TCAGTTCAAC AAGATGGACC ACTCCTGCCG AGCATGTGAA 
GATGGATATA GGCTTGAAAA TGAAACCTGC ATGAGTTGCC CATTTGGCCT TGGTGGTCTC 
AACTGTGGAA ACCCCTATCA GCTTATCACT GTGGTGATCG CAGCCGCGGG AGGTGGGCTC 
CTGCTCATCC TAGGCATCGC ACTGATTGTT ACCTGTTGCA GAAAGAATAA AAATGACATA 
AGCAAACTCA TCTTCAAAAG TGGAGATTTC CAAATGTCCC CGTATGCTGA ATACCCCAAA 
AATCCTCGCT CACAAGAATG GGGCCGAGAA GCTATTGAAA TGCATGAGAA TGGAAGTACC 
AAAAACCTCC TCCAGATGAC GGATGTGTAC TACTCGCCTA CAAGTGTAAG GAATCCAGAA 
CTTGAACGAA ACGGACTCTA CCCGGCCTAC ACTGGACTGC CAGGATCACG GCATTCTTGC 
ATTTTCCCCG GACAGTATAA CCCGTCTTTC ATCAGTGATG AAAGCAGAAG AAGAGACTAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1.
Scientific reports8(1)14251(2018 09)
Matsuura R,Kaji H,Tomioka A,Sato T,Narimatsu H,Moriwaki Y,Misawa H,Imai K,Tsuji S

HEG1 is a novel mucin-like membrane protein that serves as a diagnostic and therapeutic target for malignant mesothelioma.
Scientific reports745768(2017 03)
Tsuji S,Washimi K,Kageyama T,Yamashita M,Yoshihara M,Matsuura R,Yokose T,Kameda Y,Hayashi H,Morohoshi T,Tsuura Y,Yusa T,Sato T,Togayachi A,Narimatsu H,Nagasaki T,Nakamoto K,Moriwaki Y,Misawa H,Hiroshima K,Miyagi Y,Imai K

Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity.
eLife5e11394(2016 Jan)
de Kreuk BJ,Gingras AR,Knight JD,Liu JJ,Gingras AC,Ginsberg MH

Cerebral cavernous malformations arise independent of the heart of glass receptor.
Stroke45(5)1505-1509(2014 May)
Zheng X,Riant F,Bergametti F,Myers CD,Tang AT,Kleaveland B,Pan W,Yang J,Tournier-Lasserve E,Kahn ML

The structure of the ternary complex of Krev interaction trapped 1 (KRIT1) bound to both the Rap1 GTPase and the heart of glass (HEG1) cytoplasmic tail.
The Journal of biological chemistry288(33)23639-49(2013 Aug)
Gingras AR,Puzon-McLaughlin W,Ginsberg MH

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HEG1 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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