SPC25 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPC25
Entrez Gene ID	57405
Full Name	SPC25, NDC80 kinetochore complex component
Synonyms	AD024,SPBC25,hSpc25
General protein information	Preferred Names SPC25, NDC80 kinetochore complex component Names kinetochore protein Spc25 2600017H08Rik SPC25, NDC80 kinetochore complex component, homolog spindle pole body component 25 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q24.3
Summary	This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq, Jul 2008].
Disorder MIM:	609395

mRNA and Protein(s)

mRNA	Protein	Name
NM_020675.4	NP_065726.1	kinetochore protein Spc25
XM_011511516.2	XP_011509818.1	kinetochore protein Spc25 isoform X1
NM_020675.3	NP_065726.1	kinetochore protein Spc25

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	spc25	NP_001005041.1
Homo sapiens (human)	SPC25	NP_065726.1
Macaca mulatta (Rhesus monkey)	SPC25	XP_001103181.2
Gallus gallus (chicken)	SPC25	NP_001264786.1
Pan troglodytes (chimpanzee)	SPC25	XP_526099.2
Bos taurus (cattle)	SPC25	NP_001029402.1
Canis lupus familiaris (dog)	SPC25	XP_545510.3
Mus musculus (house mouse)	Spc25	NP_001186052.1
Rattus norvegicus (Norway rat)	Spc25	NP_001009654.1
Danio rerio (zebrafish)	spc25	NP_001116529.1

Gene Ontology
Bibliography

Related articles in PubMed

Spindle pole body component 25 regulates stemness of prostate cancer cells.
Cui F, Tang H, Tan J, Hu J
Aging10(11)3273-3282(2018 11)

Spindle pole body component 25 homolog expressed by ECM stiffening is required for lung cancer cell proliferation.
Jeong J, Keum S, Kim D, You E, Ko P, Lee J, Kim J, Kim JW, Rhee S
Biochemical and biophysical research communications500(4)937-943(2018 06)

SPC25 upregulation increases cancer stem cell properties in non-small cell lung adenocarcinoma cells and independently predicts poor survival.
Chen J, Chen H, Yang H, Dai H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie100233-239(2018 Apr)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Combined Inhibition of DNMT and HDAC Blocks the Tumorigenicity of Cancer Stem-like Cells and Attenuates Mammary Tumor Growth.
Pathania R, Ramachandran S, Mariappan G, Thakur P, Shi H, Choi JH, Manicassamy S, Kolhe R, Prasad PD, Sharma S, Lokeshwar BL, Ganapathy V, Thangaraju M
Cancer research76(11)3224-35(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPC25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPC25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu70437	XM_011511516.2 Latest version!	Homo sapiens SPC25 component of NDC80 kinetochore complex (SPC25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu31192	NM_020675.4 Latest version!	Homo sapiens SPC25 component of NDC80 kinetochore complex (SPC25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu31192	NM_020675.3	Homo sapiens SPC25, NDC80 kinetochore complex component (SPC25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPC25 ( XM_011511516.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509818.1 Homo sapiens SPC25 component of NDC80 kinetochore complex (SPC25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu70437
Clone ID Related Accession (Same CDS sequence)	XM_011511516.2
Accession Version	XM_011511516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	kinetochore protein Spc25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGAGG ACGAACTGGC ACTTTTCGAT AAAAGCATAA ATGAATTTTG GAATAAATTC 
AAAAGTACGG ACACCTCCTG TCAGATGGCG GGACTAAGAG ATACCTACAA GGATTCCATC 
AAAGCATTTG CAGAAAAGCT GTCTGTGAAA TTAAAGGAAG AAGAACGAAT GGTTGAGATG 
TTTCTGGAAT ATCAAAATCA GATCAGCAGG CAAAATAAGC TCATTCAAGA AAAAAAGGAT 
AACTTGTTAA AATTGATTGC TGAAGTAAAA GGCAAAAAGC AGGAATTGGA AGTACTGACT 
GCAAATATCC AGGATCTTAA GGAAGAATAT TCTAGGAAGA AGGAAACTAT TTCTACTGCT 
AATAAAGCGA ATGCAGAGAG GTTGAAAAGG CTGCAGAAAT CTGCAGACTT GTATAAAGAT 
CGACTTGGAC TAGAAATTCG AAAAATTTAT GGTGAGAAAT TGCAGTTTAT TTTCACTAAT 
ATTGACCCTA AGAATCCTGA GAGCCCATTT ATGTTTTCCT TACATCTCAA TGAAGCAAGG 
GACTATGAAG GCTCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509818.1
CDS	141..701
Translation	MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEGSA

Target ORF information:

RefSeq Version	XM_011511516.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SPC25 component of NDC80 kinetochore complex (SPC25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511516.2

ORF Insert Sequence:

ATGGTAGAGG ACGAACTGGC ACTTTTCGAT AAAAGCATAA ATGAATTTTG GAATAAATTC 
AAAAGTACGG ACACCTCCTG TCAGATGGCG GGACTAAGAG ATACCTACAA GGATTCCATC 
AAAGCATTTG CAGAAAAGCT GTCTGTGAAA TTAAAGGAAG AAGAACGAAT GGTTGAGATG 
TTTCTGGAAT ATCAAAATCA GATCAGCAGG CAAAATAAGC TCATTCAAGA AAAAAAGGAT 
AACTTGTTAA AATTGATTGC TGAAGTAAAA GGCAAAAAGC AGGAATTGGA AGTACTGACT 
GCAAATATCC AGGATCTTAA GGAAGAATAT TCTAGGAAGA AGGAAACTAT TTCTACTGCT 
AATAAAGCGA ATGCAGAGAG GTTGAAAAGG CTGCAGAAAT CTGCAGACTT GTATAAAGAT 
CGACTTGGAC TAGAAATTCG AAAAATTTAT GGTGAGAAAT TGCAGTTTAT TTTCACTAAT 
ATTGACCCTA AGAATCCTGA GAGCCCATTT ATGTTTTCCT TACATCTCAA TGAAGCAAGG 
GACTATGAAG GCTCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPC25 ( NM_020675.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065726.1 Homo sapiens SPC25 component of NDC80 kinetochore complex (SPC25), mRNA.

Price & Availability↑

CloneID	OHu31192
Clone ID Related Accession (Same CDS sequence)	NM_020675.4 , NM_020675.3
Accession Version	NM_020675.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	kinetochore protein Spc25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069137.6. On Nov 26, 2018 this sequence version replaced NM_020675.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.518957.1, BC022255.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000282074.7/ ENSP00000282074.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTAGAGG ACGAACTGGC ACTTTTCGAT AAAAGCATAA ATGAATTTTG GAATAAATTC 
AAAAGTACGG ACACCTCCTG TCAGATGGCG GGACTAAGAG ATACCTACAA GGATTCCATC 
AAAGCATTTG CAGAAAAGCT GTCTGTGAAA TTAAAGGAAG AAGAACGAAT GGTTGAGATG 
TTTCTGGAAT ATCAAAATCA GATCAGCAGG CAAAATAAGC TCATTCAAGA AAAAAAGGAT 
AACTTGTTAA AATTGATTGC TGAAGTAAAA GGCAAAAAGC AGGAATTGGA AGTACTGACT 
GCAAATATCC AGGATCTTAA GGAAGAATAT TCTAGGAAGA AGGAAACTAT TTCTACTGCT 
AATAAAGCGA ATGCAGAGAG GTTGAAAAGG CTGCAGAAAT CTGCAGACTT GTATAAAGAT 
CGACTTGGAC TAGAAATTCG AAAAATTTAT GGTGAGAAAT TGCAGTTTAT TTTCACTAAT 
ATTGACCCTA AGAATCCTGA GAGCCCATTT ATGTTTTCCT TACATCTCAA TGAAGCAAGG 
GACTATGAAG TGTCAGATAG TGCCCCTCAT CTTGAGGGCC TAGCAGAATT TCAAGAGAAT 
GTAAGGAAGA CCAACAATTT TTCAGCTTTT CTTGCCAATG TTCGGAAAGC TTTTACTGCC 
ACGGTTTATA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065726.1
CDS	128..802
Translation	MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN

Target ORF information:

RefSeq Version	NM_020675.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SPC25 component of NDC80 kinetochore complex (SPC25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020675.4

ORF Insert Sequence:

ATGGTAGAGG ACGAACTGGC ACTTTTCGAT AAAAGCATAA ATGAATTTTG GAATAAATTC 
AAAAGTACGG ACACCTCCTG TCAGATGGCG GGACTAAGAG ATACCTACAA GGATTCCATC 
AAAGCATTTG CAGAAAAGCT GTCTGTGAAA TTAAAGGAAG AAGAACGAAT GGTTGAGATG 
TTTCTGGAAT ATCAAAATCA GATCAGCAGG CAAAATAAGC TCATTCAAGA AAAAAAGGAT 
AACTTGTTAA AATTGATTGC TGAAGTAAAA GGCAAAAAGC AGGAATTGGA AGTACTGACT 
GCAAATATCC AGGATCTTAA GGAAGAATAT TCTAGGAAGA AGGAAACTAT TTCTACTGCT 
AATAAAGCGA ATGCAGAGAG GTTGAAAAGG CTGCAGAAAT CTGCAGACTT GTATAAAGAT 
CGACTTGGAC TAGAAATTCG AAAAATTTAT GGTGAGAAAT TGCAGTTTAT TTTCACTAAT 
ATTGACCCTA AGAATCCTGA GAGCCCATTT ATGTTTTCCT TACATCTCAA TGAAGCAAGG 
GACTATGAAG TGTCAGATAG TGCCCCTCAT CTTGAGGGCC TAGCAGAATT TCAAGAGAAT 
GTAAGGAAGA CCAACAATTT TTCAGCTTTT CTTGCCAATG TTCGGAAAGC TTTTACTGCC 
ACGGTTTATA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPC25 ( NM_020675.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065726.1 Homo sapiens SPC25, NDC80 kinetochore complex component (SPC25), mRNA.

Price & Availability↑

CloneID	OHu31192
Clone ID Related Accession (Same CDS sequence)	NM_020675.4 , NM_020675.3
Accession Version	NM_020675.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506700800000
Organism	Homo sapiens(human)
Product	kinetochore protein Spc25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022255.1. On Oct 7, 2002 this sequence version replaced NM_020675.2. ##Evidence-Data-START## Transcript exon combination :: BC022255.1, SRR1163658.385197.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTAGAGG ACGAACTGGC ACTTTTCGAT AAAAGCATAA ATGAATTTTG GAATAAATTC 
AAAAGTACGG ACACCTCCTG TCAGATGGCG GGACTAAGAG ATACCTACAA GGATTCCATC 
AAAGCATTTG CAGAAAAGCT GTCTGTGAAA TTAAAGGAAG AAGAACGAAT GGTTGAGATG 
TTTCTGGAAT ATCAAAATCA GATCAGCAGG CAAAATAAGC TCATTCAAGA AAAAAAGGAT 
AACTTGTTAA AATTGATTGC TGAAGTAAAA GGCAAAAAGC AGGAATTGGA AGTACTGACT 
GCAAATATCC AGGATCTTAA GGAAGAATAT TCTAGGAAGA AGGAAACTAT TTCTACTGCT 
AATAAAGCGA ATGCAGAGAG GTTGAAAAGG CTGCAGAAAT CTGCAGACTT GTATAAAGAT 
CGACTTGGAC TAGAAATTCG AAAAATTTAT GGTGAGAAAT TGCAGTTTAT TTTCACTAAT 
ATTGACCCTA AGAATCCTGA GAGCCCATTT ATGTTTTCCT TACATCTCAA TGAAGCAAGG 
GACTATGAAG TGTCAGATAG TGCCCCTCAT CTTGAGGGCC TAGCAGAATT TCAAGAGAAT 
GTAAGGAAGA CCAACAATTT TTCAGCTTTT CTTGCCAATG TTCGGAAAGC TTTTACTGCC 
ACGGTTTATA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065726.1
CDS	132..806
Translation	MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN

Target ORF information:

RefSeq Version	NM_020675.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPC25, NDC80 kinetochore complex component (SPC25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020675.3

ORF Insert Sequence:

ATGGTAGAGG ACGAACTGGC ACTTTTCGAT AAAAGCATAA ATGAATTTTG GAATAAATTC 
AAAAGTACGG ACACCTCCTG TCAGATGGCG GGACTAAGAG ATACCTACAA GGATTCCATC 
AAAGCATTTG CAGAAAAGCT GTCTGTGAAA TTAAAGGAAG AAGAACGAAT GGTTGAGATG 
TTTCTGGAAT ATCAAAATCA GATCAGCAGG CAAAATAAGC TCATTCAAGA AAAAAAGGAT 
AACTTGTTAA AATTGATTGC TGAAGTAAAA GGCAAAAAGC AGGAATTGGA AGTACTGACT 
GCAAATATCC AGGATCTTAA GGAAGAATAT TCTAGGAAGA AGGAAACTAT TTCTACTGCT 
AATAAAGCGA ATGCAGAGAG GTTGAAAAGG CTGCAGAAAT CTGCAGACTT GTATAAAGAT 
CGACTTGGAC TAGAAATTCG AAAAATTTAT GGTGAGAAAT TGCAGTTTAT TTTCACTAAT 
ATTGACCCTA AGAATCCTGA GAGCCCATTT ATGTTTTCCT TACATCTCAA TGAAGCAAGG 
GACTATGAAG TGTCAGATAG TGCCCCTCAT CTTGAGGGCC TAGCAGAATT TCAAGAGAAT 
GTAAGGAAGA CCAACAATTT TTCAGCTTTT CTTGCCAATG TTCGGAAAGC TTTTACTGCC 
ACGGTTTATA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spindle pole body component 25 regulates stemness of prostate cancer cells.
Aging10(11)3273-3282(2018 11)
Cui F,Tang H,Tan J,Hu J

Spindle pole body component 25 homolog expressed by ECM stiffening is required for lung cancer cell proliferation.
Biochemical and biophysical research communications500(4)937-943(2018 06)
Jeong J,Keum S,Kim D,You E,Ko P,Lee J,Kim J,Kim JW,Rhee S

SPC25 upregulation increases cancer stem cell properties in non-small cell lung adenocarcinoma cells and independently predicts poor survival.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie100233-239(2018 Apr)
Chen J,Chen H,Yang H,Dai H

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Combined Inhibition of DNMT and HDAC Blocks the Tumorigenicity of Cancer Stem-like Cells and Attenuates Mammary Tumor Growth.
Cancer research76(11)3224-35(2016 06)
Pathania R,Ramachandran S,Mariappan G,Thakur P,Shi H,Choi JH,Manicassamy S,Kolhe R,Prasad PD,Sharma S,Lokeshwar BL,Ganapathy V,Thangaraju M

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SPC25 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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