VARS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS2
Entrez Gene ID	57176
Full Name	valyl-tRNA synthetase 2, mitochondrial
Synonyms	COXPD20,VALRS,VARS2L,VARSL
General protein information	Preferred Names valyl-tRNA synthetase 2, mitochondrial Names valine--tRNA ligase, mitochondrial valine tRNA ligase 2, mitochondrial (putative) valyl-tRNA synthetase like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014].
Disorder MIM:	612802
Disorder Html:	Combined oxidative phosphorylation deficiency 20, 615917 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_020442.5	NP_065175.4	valine--tRNA ligase, mitochondrial isoform 2 precursor
NM_001167733.2	NP_001161205.1	valine--tRNA ligase, mitochondrial isoform 3
NM_001167734.1	NP_001161206.1	valine--tRNA ligase, mitochondrial isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Vars2	NP_780346.3
Rattus norvegicus (Norway rat)	Vars2	NP_998728.1
Bos taurus (cattle)	VARS2	NP_001243473.1
Danio rerio (zebrafish)	vars2	NP_001124133.1
Xenopus tropicalis (tropical clawed frog)	LOC100493727	XP_004917090.1
Homo sapiens (human)	VARS2	NP_001161206.1
Canis lupus familiaris (dog)	VARS2	XP_005627180.1

Gene Ontology
Bibliography

Related articles in PubMed

VARS2-linked mitochondrial encephalopathy: two case reports enlarging the clinical phenotype.
Begliuomini C, Magli G, Di Rocco M, Santorelli FM, Cassandrini D, Nesti C, Deodato F, Diodato D, Casellato S, Simula DM, Dessì V, Eusebi A, Carta A, Sotgiu S
BMC medical genetics20(1)77(2019 05)

A novel compound heterozygous mutation in VARS2 in a newborn with mitochondrial cardiomyopathy: a case report of a Chinese family.
Ma K, Xie M, He X, Liu G, Lu X, Peng Q, Zhong B, Li N
BMC medical genetics19(1)202(2018 11)

Clinical, biochemical, and genetic features associated with VARS2-related mitochondrial disease.
Bruni F, Di Meo I, Bellacchio E, Webb BD, McFarland R, Chrzanowska-Lightowlers ZMA, He L, Skorupa E, Moroni I, Ardissone A, Walczak A, Tyynismaa H, Isohanni P, Mandel H, Prokisch H, Haack T, Bonnen PE, Enrico B, Pronicka E, Ghezzi D, Taylor RW, Diodato D
Human mutation39(4)563-578(2018 04)

Recessive VARS2 mutation underlies a novel syndrome with epilepsy, mental retardation, short stature, growth hormone deficiency, and hypogonadism.
Alsemari A, Al-Younes B, Goljan E, Jaroudi D, BinHumaid F, Meyer BF, Arold ST, Monies D
Human genomics11(1)28(2017 Nov)

VARS2 and TARS2 mutations in patients with mitochondrial encephalomyopathies.
Diodato D, Melchionda L, Haack TB, Dallabona C, Baruffini E, Donnini C, Granata T, Ragona F, Balestri P, Margollicci M, Lamantea E, Nasca A, Powell CA, Minczuk M, Strom TM, Meitinger T, Prokisch H, Lamperti C, Zeviani M, Ghezzi D
Human mutation35(8)983-9(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08773	NM_001167733.2 Latest version!	Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu08579	NM_020442.5 Latest version!	Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu12904	NM_001167734.1 Latest version!	Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS2 ( NM_001167733.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001161205.1 Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu08773
Clone ID Related Accession (Same CDS sequence)	NM_001167733.2
Accession Version	NM_001167733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570377600000
Organism	Homo sapiens(human)
Product	valine--tRNA ligase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC307585.1, AK293780.1, AK303415.1 and BC073838.1. On Aug 7, 2014 this sequence version replaced NM_001167733.1. Transcript Variant: This variant (3) contains alternate 5' exon structure, and it thus differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCGGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCGGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTGTT 
GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAATGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGCGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGCGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCCTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGCCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GACAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161205.1
CDS	357..3128
Translation	MCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSEWGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	NM_001167733.2
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167733.2

ORF Insert Sequence:

ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCGGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCGGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTGTT 
GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAATGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGCGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGCGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCCTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGCCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GACAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( NM_020442.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065175.4 Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08579
Clone ID Related Accession (Same CDS sequence)	NM_020442.5
Accession Version	NM_020442.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570377600000
Organism	Homo sapiens(human)
Product	valine--tRNA ligase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC307585.1, AK303415.1, AK294451.1 and BC073838.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2014 this sequence version replaced NM_020442.4. Transcript Variant: This variant (2) contains alternate 5' exon structure, and it thus differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCGGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCGGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTGTT 
GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAATGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGCGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGCGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCCTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGCCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GACAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065175.4
CDS	165..3356
Translation	MPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSEWGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	NM_020442.5
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020442.5

ORF Insert Sequence:

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCGGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCGGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTGTT 
GCCTTCCCCG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAATGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGCGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGCGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCCTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGCCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GACAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( NM_001167734.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001161206.1 Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 1, mRNA.

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CloneID	OHu12904
Clone ID Related Accession (Same CDS sequence)	NM_001167734.1
Accession Version	NM_001167734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570377600000
Organism	Homo sapiens(human)
Product	valine--tRNA ligase, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304807.1, AL669830.9, BC112054.1 and BC073838.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGGA AGGCTTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCGGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCGGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTGTT GCCTTCCCCG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAATGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAC 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGCGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGCGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCCTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGAGG CTGTGAAGCC CGTGCTGTGG 
CACTCGCCCC GCCCCCTGGG GCCCCCTCAG GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC 
CGCCTCCTGG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC 
AGGCCTGGTT GCCCCCCTGC CCCCAGCATC TCGGTTGCCC CCTACCCCAG CGCCTGCAGC 
TTGGAGCACT GGCGCCAGCC AGAGCTGGAG CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG 
CAGGTGCTAA GGGCTCTCCG AGCCACGTAC CAGCTCACCA AAGCCCGGCC CCGAGTGCTG 
CTGCAGAGCT CAGAGCCTGG GGACCAGGGC CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC 
ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG TTACCCCCAG GCGCAGCAGC TCCCTCCGGC 
TGGGCCCAGG CTCCACTCAG TGACACGGCT CAAGTCTACA TGGAGCTGCA GGGCCTGGTG 
GACCCGCAGA TCCAGCTACC TCTGTTAGCC GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT 
GACAGCCTCA CAGCCAGGAC CCCATCAGAA GGGGAGGCAG GGACTCAGAG GCAACAAAAG 
CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA CTGGACAAGG CAGCCTCTCA CCTCCGGCAG 
CTGATGGATG AGCCTCCAGC CCCAGGGAGC CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161206.1
CDS	82..3363
Translation	MGGKAWPRRAVGTAGGPCAEQISAPFQTLLMPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSEWGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	NM_001167734.1
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167734.1

ORF Insert Sequence:

ATGGGCGGGA AGGCTTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCGGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCGGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTGTT GCCTTCCCCG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAATGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAC 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGCGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGCGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCCTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGAGG CTGTGAAGCC CGTGCTGTGG 
CACTCGCCCC GCCCCCTGGG GCCCCCTCAG GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC 
CGCCTCCTGG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC 
AGGCCTGGTT GCCCCCCTGC CCCCAGCATC TCGGTTGCCC CCTACCCCAG CGCCTGCAGC 
TTGGAGCACT GGCGCCAGCC AGAGCTGGAG CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG 
CAGGTGCTAA GGGCTCTCCG AGCCACGTAC CAGCTCACCA AAGCCCGGCC CCGAGTGCTG 
CTGCAGAGCT CAGAGCCTGG GGACCAGGGC CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC 
ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG TTACCCCCAG GCGCAGCAGC TCCCTCCGGC 
TGGGCCCAGG CTCCACTCAG TGACACGGCT CAAGTCTACA TGGAGCTGCA GGGCCTGGTG 
GACCCGCAGA TCCAGCTACC TCTGTTAGCC GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT 
GACAGCCTCA CAGCCAGGAC CCCATCAGAA GGGGAGGCAG GGACTCAGAG GCAACAAAAG 
CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA CTGGACAAGG CAGCCTCTCA CCTCCGGCAG 
CTGATGGATG AGCCTCCAGC CCCAGGGAGC CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

VARS2-linked mitochondrial encephalopathy: two case reports enlarging the clinical phenotype.
BMC medical genetics20(1)77(2019 05)
Begliuomini C,Magli G,Di Rocco M,Santorelli FM,Cassandrini D,Nesti C,Deodato F,Diodato D,Casellato S,Simula DM,Dessì V,Eusebi A,Carta A,Sotgiu S

A novel compound heterozygous mutation in VARS2 in a newborn with mitochondrial cardiomyopathy: a case report of a Chinese family.
BMC medical genetics19(1)202(2018 11)
Ma K,Xie M,He X,Liu G,Lu X,Peng Q,Zhong B,Li N

Clinical, biochemical, and genetic features associated with VARS2-related mitochondrial disease.
Human mutation39(4)563-578(2018 04)
Bruni F,Di Meo I,Bellacchio E,Webb BD,McFarland R,Chrzanowska-Lightowlers ZMA,He L,Skorupa E,Moroni I,Ardissone A,Walczak A,Tyynismaa H,Isohanni P,Mandel H,Prokisch H,Haack T,Bonnen PE,Enrico B,Pronicka E,Ghezzi D,Taylor RW,Diodato D

Recessive VARS2 mutation underlies a novel syndrome with epilepsy, mental retardation, short stature, growth hormone deficiency, and hypogonadism.
Human genomics11(1)28(2017 Nov)
Alsemari A,Al-Younes B,Goljan E,Jaroudi D,BinHumaid F,Meyer BF,Arold ST,Monies D

VARS2 and TARS2 mutations in patients with mitochondrial encephalomyopathies.
Human mutation35(8)983-9(2014 Aug)
Diodato D,Melchionda L,Haack TB,Dallabona C,Baruffini E,Donnini C,Granata T,Ragona F,Balestri P,Margollicci M,Lamantea E,Nasca A,Powell CA,Minczuk M,Strom TM,Meitinger T,Prokisch H,Lamperti C,Zeviani M,Ghezzi D

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VARS2 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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