PI4K2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PI4K2B
Entrez Gene ID	55300
Full Name	phosphatidylinositol 4-kinase type 2 beta
Synonyms	PI4KIIB,PIK42B
General protein information	Preferred Names phosphatidylinositol 4-kinase type 2 beta Names phosphatidylinositol 4-kinase type 2-beta PI4KII-BETA phosphatidylinositol 4-kinase type-II beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p15.2
Summary	This gene encodes a member of the type II PI4 kinase protein family. The encoded protein is primarily cytosolic and contributes to overall PI4-kinase activity along with other protein family members. This protein is involved in early T cell activation. [provided by RefSeq, Dec 2016].
Disorder MIM:	612101

mRNA and Protein(s)

mRNA	Protein	Name
XM_005248174.2	XP_005248231.1	phosphatidylinositol 4-kinase type 2-beta isoform X1
NM_018323.4	NP_060793.2	phosphatidylinositol 4-kinase type 2-beta
XM_005248175.4	XP_005248232.1	phosphatidylinositol 4-kinase type 2-beta isoform X2
XM_005248174.1	XP_005248231.1	phosphatidylinositol 4-kinase type 2-beta isoform X1
NM_018323.3	NP_060793.2	phosphatidylinositol 4-kinase type 2-beta

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR087W	NP_985634.2
Homo sapiens (human)	PI4K2B	NP_060793.2
Gallus gallus (chicken)	PI4K2B	NP_001026328.1
Danio rerio (zebrafish)	pi4k2b	NP_001038950.1
Saccharomyces cerevisiae (baker's yeast)	LSB6	NP_012435.1
Kluyveromyces lactis	KLLA0F14080g	XP_455712.1
Neurospora crassa	NCU04355	XP_955827.1
Xenopus tropicalis (tropical clawed frog)	pi4k2b	NP_001016953.1
Magnaporthe oryzae (rice blast fungus)	MGG_07151	XP_003715349.1
Macaca mulatta (Rhesus monkey)	LOC693561	XP_002808453.1
Bos taurus (cattle)	PI4K2B	NP_001095534.1
Mus musculus (house mouse)	Pi4k2b	NP_080227.2
Rattus norvegicus (Norway rat)	Pi4k2b	NP_001005883.1
Schizosaccharomyces pombe (fission yeast)	lsb6	XP_001713056.1
Pan troglodytes (chimpanzee)	PI4K2B	XP_003310311.1
Canis lupus familiaris (dog)	PI4K2B	XP_005618711.1

Gene Ontology
Bibliography

Related articles in PubMed

Type II PI4-kinases control Weibel-Palade body biogenesis and von Willebrand factor structure in human endothelial cells.
Lopes da Silva M, O'Connor MN, Kriston-Vizi J, White IJ, Al-Shawi R, Simons JP, Mössinger J, Haucke V, Cutler DF
Journal of cell science129(10)2096-105(2016 05)

The high-resolution crystal structure of phosphatidylinositol 4-kinase IIβ and the crystal structure of phosphatidylinositol 4-kinase IIα containing a nucleoside analogue provide a structural basis for isoform-specific inhibitor design.
Klima M, Baumlova A, Chalupska D, Hřebabecký H, Dejmek M, Nencka R, Boura E
Acta crystallographica. Section D, Biological crystallography71(Pt 7)1555-63(2015 Jul)

Type II PtdIns 4-kinase β associates with CD4-p56lck complex and is involved in CD4 receptor signaling.
Sinha RK, Bojjireddy N, Kanojia D, Subrahmanyam G
Molecular and cellular biochemistry395(1-2)231-9(2014 Oct)

Type II phosphatidylinositol 4-kinases interact with FcεRIγ subunit in RBL-2H3 cells.
Bojjireddy N, Sinha RK, Subrahmanyam G
Molecular and cellular biochemistry390(1-2)197-203(2014 May)

Type II phosphatidylinositol 4-kinase β is an integral signaling component of early T cell activation mechanisms.
Sinha RK, Bojjireddy N, Kulkarni D, Ratheesh A, Chiplunkar SV, Gude R, Subrahmanyam G
Biochimie95(8)1560-6(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PI4K2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PI4K2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu38469	XM_005248174.2 Latest version!	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu38470	XM_005248175.4 Latest version!	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu32036	NM_018323.4 Latest version!	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu38469	XM_005248174.1	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu32036	NM_018323.3	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PI4K2B ( NM_018323.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060793.2 Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu32036
Clone ID Related Accession (Same CDS sequence)	NM_018323.4 , NM_018323.3
Accession Version	NM_018323.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	phosphatidylinositol 4-kinase type 2-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC051749.1, AY091514.1 and AC108218.3. This sequence is a reference standard in the RefSeqGene project. On Mar 6, 2009 this sequence version replaced NM_018323.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051749.1, AY091514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGATTG GTTCCTTTCA GTTATTTGTT 
GAAGGTTACA AGGAGGCTGA ATATTGGCTT AGGAAATTTG AAGCTGACCC TTTGCCTGAG 
AATATTAGAA AACAATTTCA GTCACAATTT GAAAGATTAG TTATTTTGGA TTACATCATC 
AGAAATACAG ACAGGGGCAA TGATAATTGG TTAGTCAGAT ACGAAAAGCA GAAATGTGAA 
AAGGAAATTG ACCATAAGGA ATCAAAATGG ATTGATGATG AAGAATTCCT TATTAAAATA 
GCTGCAATTG ATAATGGTCT AGCATTTCCT TTTAAACATC CTGATGAATG GAGAGCATAT 
CCATTTCACT GGGCTTGGCT TCCTCAAGCA AAAGTTCCCT TTTCTGAAGA AATAAGAAAT 
TTGATTCTAC CATATATTTC TGACATGAAC TTTGTGCAAG ATTTATGTGA AGATCTCTAT 
GAACTTTTTA AGACTGACAA AGGATTTGAC AAAGCCACTT TTGAAAGTCA GATGTCTGTG 
ATGAGGGGTC AGATCTTAAA CCTTACTCAG GCATTGAGAG ACGGGAAGAG TCCTTTCCAG 
CTAGTACAGA TACCTTGTGT GATTGTGGAA CGCAGTCAAG GTGGAAGTCA GGGTCGGATT 
GTCCACCTGA GCAATTCCTT TACCCAGACT GTCAATTGCA GGAAGCCATT TTTTTCCTCC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060793.2
CDS	134..1579
Translation	MEDPSEPDRLASADGGSPEEEEDGEREPLLPRIAWAHPRRGAPGSAVRLLDAAGEEGEAGDEELPLPPGDVGVSRSSSAELDRSRPAVSVTIGTSEMNAFLDDPEFADIMLRAEQAIEVGIFPERISQGSSGSYFVKDPKRKIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKIGSFQLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDEEFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW

Target ORF information:

RefSeq Version	NM_018323.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018323.3

ORF Insert Sequence:

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGATTG GTTCCTTTCA GTTATTTGTT 
GAAGGTTACA AGGAGGCTGA ATATTGGCTT AGGAAATTTG AAGCTGACCC TTTGCCTGAG 
AATATTAGAA AACAATTTCA GTCACAATTT GAAAGATTAG TTATTTTGGA TTACATCATC 
AGAAATACAG ACAGGGGCAA TGATAATTGG TTAGTCAGAT ACGAAAAGCA GAAATGTGAA 
AAGGAAATTG ACCATAAGGA ATCAAAATGG ATTGATGATG AAGAATTCCT TATTAAAATA 
GCTGCAATTG ATAATGGTCT AGCATTTCCT TTTAAACATC CTGATGAATG GAGAGCATAT 
CCATTTCACT GGGCTTGGCT TCCTCAAGCA AAAGTTCCCT TTTCTGAAGA AATAAGAAAT 
TTGATTCTAC CATATATTTC TGACATGAAC TTTGTGCAAG ATTTATGTGA AGATCTCTAT 
GAACTTTTTA AGACTGACAA AGGATTTGAC AAAGCCACTT TTGAAAGTCA GATGTCTGTG 
ATGAGGGGTC AGATCTTAAA CCTTACTCAG GCATTGAGAG ACGGGAAGAG TCCTTTCCAG 
CTAGTACAGA TACCTTGTGT GATTGTGGAA CGCAGTCAAG GTGGAAGTCA GGGTCGGATT 
GTCCACCTGA GCAATTCCTT TACCCAGACT GTCAATTGCA GGAAGCCATT TTTTTCCTCC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI4K2B ( XM_005248174.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248231.1 Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu38469
Clone ID Related Accession (Same CDS sequence)	XM_005248174.2 , XM_005248174.1
Accession Version	XM_005248174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	phosphatidylinositol 4-kinase type 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGTTAT TTGTTGAAGG TTACAAGGAG 
GCTGAATATT GGCTTAGGAA ATTTGAAGCT GACCCTTTGC CTGAGAATAT TAGAAAACAA 
TTTCAGTCAC AATTTGAAAG ATTAGTTATT TTGGATTACA TCATCAGAAA TACAGACAGG 
GGCAATGATA ATTGGTTAGT CAGATACGAA AAGCAGAAAT GTGAAAAGGA AATTGACCAT 
AAGGAATCAA AATGGATTGA TGATGAAGAA TTCCTTATTA AAATAGCTGC AATTGATAAT 
GGTCTAGCAT TTCCTTTTAA ACATCCTGAT GAATGGAGAG CATATCCATT TCACTGGGCT 
TGGCTTCCTC AAGCAAAAGT TCCCTTTTCT GAAGAAATAA GAAATTTGAT TCTACCATAT 
ATTTCTGACA TGAACTTTGT GCAAGATTTA TGTGAAGATC TCTATGAACT TTTTAAGACT 
GACAAAGGAT TTGACAAAGC CACTTTTGAA AGTCAGATGT CTGTGATGAG GGGTCAGATC 
TTAAACCTTA CTCAGGCATT GAGAGACGGG AAGAGTCCTT TCCAGCTAGT ACAGATACCT 
TGTGTGATTG TGGAACGCAG TCAAGGTGGA AGTCAGGGTC GGATTGTCCA CCTGAGCAAT 
TCCTTTACCC AGACTGTCAA TTGCAGGAAG CCATTTTTTT CCTCCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248231.1
CDS	122..1552
Translation	MEDPSEPDRLASADGGSPEEEEDGEREPLLPRIAWAHPRRGAPGSAVRLLDAAGEEGEAGDEELPLPPGDVGVSRSSSAELDRSRPAVSVTIGTSEMNAFLDDPEFADIMLRAEQAIEVGIFPERISQGSSGSYFVKDPKRKIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDEEFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW

Target ORF information:

RefSeq Version	XM_005248174.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248174.2

ORF Insert Sequence:

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGTTAT TTGTTGAAGG TTACAAGGAG 
GCTGAATATT GGCTTAGGAA ATTTGAAGCT GACCCTTTGC CTGAGAATAT TAGAAAACAA 
TTTCAGTCAC AATTTGAAAG ATTAGTTATT TTGGATTACA TCATCAGAAA TACAGACAGG 
GGCAATGATA ATTGGTTAGT CAGATACGAA AAGCAGAAAT GTGAAAAGGA AATTGACCAT 
AAGGAATCAA AATGGATTGA TGATGAAGAA TTCCTTATTA AAATAGCTGC AATTGATAAT 
GGTCTAGCAT TTCCTTTTAA ACATCCTGAT GAATGGAGAG CATATCCATT TCACTGGGCT 
TGGCTTCCTC AAGCAAAAGT TCCCTTTTCT GAAGAAATAA GAAATTTGAT TCTACCATAT 
ATTTCTGACA TGAACTTTGT GCAAGATTTA TGTGAAGATC TCTATGAACT TTTTAAGACT 
GACAAAGGAT TTGACAAAGC CACTTTTGAA AGTCAGATGT CTGTGATGAG GGGTCAGATC 
TTAAACCTTA CTCAGGCATT GAGAGACGGG AAGAGTCCTT TCCAGCTAGT ACAGATACCT 
TGTGTGATTG TGGAACGCAG TCAAGGTGGA AGTCAGGGTC GGATTGTCCA CCTGAGCAAT 
TCCTTTACCC AGACTGTCAA TTGCAGGAAG CCATTTTTTT CCTCCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI4K2B ( XM_005248174.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248231.1 Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu38469
Clone ID Related Accession (Same CDS sequence)	XM_005248174.2 , XM_005248174.1
Accession Version	XM_005248174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	phosphatidylinositol 4-kinase type 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGTTAT TTGTTGAAGG TTACAAGGAG 
GCTGAATATT GGCTTAGGAA ATTTGAAGCT GACCCTTTGC CTGAGAATAT TAGAAAACAA 
TTTCAGTCAC AATTTGAAAG ATTAGTTATT TTGGATTACA TCATCAGAAA TACAGACAGG 
GGCAATGATA ATTGGTTAGT CAGATACGAA AAGCAGAAAT GTGAAAAGGA AATTGACCAT 
AAGGAATCAA AATGGATTGA TGATGAAGAA TTCCTTATTA AAATAGCTGC AATTGATAAT 
GGTCTAGCAT TTCCTTTTAA ACATCCTGAT GAATGGAGAG CATATCCATT TCACTGGGCT 
TGGCTTCCTC AAGCAAAAGT TCCCTTTTCT GAAGAAATAA GAAATTTGAT TCTACCATAT 
ATTTCTGACA TGAACTTTGT GCAAGATTTA TGTGAAGATC TCTATGAACT TTTTAAGACT 
GACAAAGGAT TTGACAAAGC CACTTTTGAA AGTCAGATGT CTGTGATGAG GGGTCAGATC 
TTAAACCTTA CTCAGGCATT GAGAGACGGG AAGAGTCCTT TCCAGCTAGT ACAGATACCT 
TGTGTGATTG TGGAACGCAG TCAAGGTGGA AGTCAGGGTC GGATTGTCCA CCTGAGCAAT 
TCCTTTACCC AGACTGTCAA TTGCAGGAAG CCATTTTTTT CCTCCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248231.1
CDS	132..1562
Translation	MEDPSEPDRLASADGGSPEEEEDGEREPLLPRIAWAHPRRGAPGSAVRLLDAAGEEGEAGDEELPLPPGDVGVSRSSSAELDRSRPAVSVTIGTSEMNAFLDDPEFADIMLRAEQAIEVGIFPERISQGSSGSYFVKDPKRKIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDEEFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW

Target ORF information:

RefSeq Version	XM_005248174.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248174.1

ORF Insert Sequence:

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGTTAT TTGTTGAAGG TTACAAGGAG 
GCTGAATATT GGCTTAGGAA ATTTGAAGCT GACCCTTTGC CTGAGAATAT TAGAAAACAA 
TTTCAGTCAC AATTTGAAAG ATTAGTTATT TTGGATTACA TCATCAGAAA TACAGACAGG 
GGCAATGATA ATTGGTTAGT CAGATACGAA AAGCAGAAAT GTGAAAAGGA AATTGACCAT 
AAGGAATCAA AATGGATTGA TGATGAAGAA TTCCTTATTA AAATAGCTGC AATTGATAAT 
GGTCTAGCAT TTCCTTTTAA ACATCCTGAT GAATGGAGAG CATATCCATT TCACTGGGCT 
TGGCTTCCTC AAGCAAAAGT TCCCTTTTCT GAAGAAATAA GAAATTTGAT TCTACCATAT 
ATTTCTGACA TGAACTTTGT GCAAGATTTA TGTGAAGATC TCTATGAACT TTTTAAGACT 
GACAAAGGAT TTGACAAAGC CACTTTTGAA AGTCAGATGT CTGTGATGAG GGGTCAGATC 
TTAAACCTTA CTCAGGCATT GAGAGACGGG AAGAGTCCTT TCCAGCTAGT ACAGATACCT 
TGTGTGATTG TGGAACGCAG TCAAGGTGGA AGTCAGGGTC GGATTGTCCA CCTGAGCAAT 
TCCTTTACCC AGACTGTCAA TTGCAGGAAG CCATTTTTTT CCTCCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI4K2B ( XM_005248175.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248232.1 Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X2, mRNA.

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CloneID	OHu38470
Clone ID Related Accession (Same CDS sequence)	XM_005248175.4
Accession Version	XM_005248175.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	phosphatidylinositol 4-kinase type 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248175.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAT TCTTGGATGA CCCAGAATTT GCCGATATTA TGCTGAGAGC AGAGCAAGCA 
ATAGAAGTTG GAATTTTTCC AGAAAGAATC TCTCAAGGTT CAAGTGGAAG TTACTTTGTG 
AAGGATCCTA AGAGGAAAAT TATTGGTGTG TTTAAACCCA AATCAGAAGA GCCTTATGGT 
CAACTCAATC CAAAATGGAC CAAATATGTC CATAAGGTCT GCTGCCCTTG CTGCTTTGGC 
CGAGGCTGCC TGATTCCTAA TCAGGGGTAC CTTTCCGAAG CGGGTGCCTA TCTTGTGGAC 
AACAAGCTTC ATCTGAGCAT TGTACCTAAA ACAAAGGTGG TTTGGCTTGT CAGTGAGACA 
TTTAACTATA ATGCGATTGA CCGTGCAAAA TCAAGAGGCA AAAAGTATGC TTTAGAAAAA 
GTGCCAAAAG TGGGTAGAAA GTTTCATAGG ATAGGACTCC CTCCTAAGAT TGGTTCCTTT 
CAGTTATTTG TTGAAGGTTA CAAGGAGGCT GAATATTGGC TTAGGAAATT TGAAGCTGAC 
CCTTTGCCTG AGAATATTAG AAAACAATTT CAGTCACAAT TTGAAAGATT AGTTATTTTG 
GATTACATCA TCAGAAATAC AGACAGGGGC AATGATAATT GGTTAGTCAG ATACGAAAAG 
CAGAAATGTG AAAAGGAAAT TGACCATAAG GAATCAAAAT GGATTGATGA TGAAGAATTC 
CTTATTAAAA TAGCTGCAAT TGATAATGGT CTAGCATTTC CTTTTAAACA TCCTGATGAA 
TGGAGAGCAT ATCCATTTCA CTGGGCTTGG CTTCCTCAAG CAAAAGTTCC CTTTTCTGAA 
GAAATAAGAA ATTTGATTCT ACCATATATT TCTGACATGA ACTTTGTGCA AGATTTATGT 
GAAGATCTCT ATGAACTTTT TAAGACTGAC AAAGGATTTG ACAAAGCCAC TTTTGAAAGT 
CAGATGTCTG TGATGAGGGG TCAGATCTTA AACCTTACTC AGGCATTGAG AGACGGGAAG 
AGTCCTTTCC AGCTAGTACA GATACCTTGT GTGATTGTGG AACGCAGTCA AGGTGGAAGT 
CAGGGTCGGA TTGTCCACCT GAGCAATTCC TTTACCCAGA CTGTCAATTG CAGGAAGCCA 
TTTTTTTCCT CCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248232.1
CDS	200..1357
Translation	MNAFLDDPEFADIMLRAEQAIEVGIFPERISQGSSGSYFVKDPKRKIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKIGSFQLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDEEFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW

Target ORF information:

RefSeq Version	XM_005248175.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248175.4

ORF Insert Sequence:

ATGAATGCAT TCTTGGATGA CCCAGAATTT GCCGATATTA TGCTGAGAGC AGAGCAAGCA 
ATAGAAGTTG GAATTTTTCC AGAAAGAATC TCTCAAGGTT CAAGTGGAAG TTACTTTGTG 
AAGGATCCTA AGAGGAAAAT TATTGGTGTG TTTAAACCCA AATCAGAAGA GCCTTATGGT 
CAACTCAATC CAAAATGGAC CAAATATGTC CATAAGGTCT GCTGCCCTTG CTGCTTTGGC 
CGAGGCTGCC TGATTCCTAA TCAGGGGTAC CTTTCCGAAG CGGGTGCCTA TCTTGTGGAC 
AACAAGCTTC ATCTGAGCAT TGTACCTAAA ACAAAGGTGG TTTGGCTTGT CAGTGAGACA 
TTTAACTATA ATGCGATTGA CCGTGCAAAA TCAAGAGGCA AAAAGTATGC TTTAGAAAAA 
GTGCCAAAAG TGGGTAGAAA GTTTCATAGG ATAGGACTCC CTCCTAAGAT TGGTTCCTTT 
CAGTTATTTG TTGAAGGTTA CAAGGAGGCT GAATATTGGC TTAGGAAATT TGAAGCTGAC 
CCTTTGCCTG AGAATATTAG AAAACAATTT CAGTCACAAT TTGAAAGATT AGTTATTTTG 
GATTACATCA TCAGAAATAC AGACAGGGGC AATGATAATT GGTTAGTCAG ATACGAAAAG 
CAGAAATGTG AAAAGGAAAT TGACCATAAG GAATCAAAAT GGATTGATGA TGAAGAATTC 
CTTATTAAAA TAGCTGCAAT TGATAATGGT CTAGCATTTC CTTTTAAACA TCCTGATGAA 
TGGAGAGCAT ATCCATTTCA CTGGGCTTGG CTTCCTCAAG CAAAAGTTCC CTTTTCTGAA 
GAAATAAGAA ATTTGATTCT ACCATATATT TCTGACATGA ACTTTGTGCA AGATTTATGT 
GAAGATCTCT ATGAACTTTT TAAGACTGAC AAAGGATTTG ACAAAGCCAC TTTTGAAAGT 
CAGATGTCTG TGATGAGGGG TCAGATCTTA AACCTTACTC AGGCATTGAG AGACGGGAAG 
AGTCCTTTCC AGCTAGTACA GATACCTTGT GTGATTGTGG AACGCAGTCA AGGTGGAAGT 
CAGGGTCGGA TTGTCCACCT GAGCAATTCC TTTACCCAGA CTGTCAATTG CAGGAAGCCA 
TTTTTTTCCT CCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI4K2B ( NM_018323.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060793.2 Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA.

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CloneID	OHu32036
Clone ID Related Accession (Same CDS sequence)	NM_018323.4 , NM_018323.3
Accession Version	NM_018323.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1566316800000
Organism	Homo sapiens(human)
Product	phosphatidylinositol 4-kinase type 2-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC051749.1, AY091514.1 and AC108218.3. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018323.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051749.1, AY091514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264864.8/ ENSP00000264864.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGATTG GTTCCTTTCA GTTATTTGTT 
GAAGGTTACA AGGAGGCTGA ATATTGGCTT AGGAAATTTG AAGCTGACCC TTTGCCTGAG 
AATATTAGAA AACAATTTCA GTCACAATTT GAAAGATTAG TTATTTTGGA TTACATCATC 
AGAAATACAG ACAGGGGCAA TGATAATTGG TTAGTCAGAT ACGAAAAGCA GAAATGTGAA 
AAGGAAATTG ACCATAAGGA ATCAAAATGG ATTGATGATG AAGAATTCCT TATTAAAATA 
GCTGCAATTG ATAATGGTCT AGCATTTCCT TTTAAACATC CTGATGAATG GAGAGCATAT 
CCATTTCACT GGGCTTGGCT TCCTCAAGCA AAAGTTCCCT TTTCTGAAGA AATAAGAAAT 
TTGATTCTAC CATATATTTC TGACATGAAC TTTGTGCAAG ATTTATGTGA AGATCTCTAT 
GAACTTTTTA AGACTGACAA AGGATTTGAC AAAGCCACTT TTGAAAGTCA GATGTCTGTG 
ATGAGGGGTC AGATCTTAAA CCTTACTCAG GCATTGAGAG ACGGGAAGAG TCCTTTCCAG 
CTAGTACAGA TACCTTGTGT GATTGTGGAA CGCAGTCAAG GTGGAAGTCA GGGTCGGATT 
GTCCACCTGA GCAATTCCTT TACCCAGACT GTCAATTGCA GGAAGCCATT TTTTTCCTCC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060793.2
CDS	132..1577
Translation	MEDPSEPDRLASADGGSPEEEEDGEREPLLPRIAWAHPRRGAPGSAVRLLDAAGEEGEAGDEELPLPPGDVGVSRSSSAELDRSRPAVSVTIGTSEMNAFLDDPEFADIMLRAEQAIEVGIFPERISQGSSGSYFVKDPKRKIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKIGSFQLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDEEFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW

Target ORF information:

RefSeq Version	NM_018323.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018323.4

ORF Insert Sequence:

ATGGAGGATC CCTCCGAGCC CGACCGGTTG GCGTCCGCGG ACGGCGGGAG CCCGGAGGAG 
GAGGAGGATG GGGAGCGGGA GCCGCTGCTA CCGCGGATCG CCTGGGCCCA CCCGCGGAGA 
GGCGCCCCAG GCAGCGCCGT GAGGCTGCTG GACGCTGCCG GGGAGGAGGG CGAGGCCGGC 
GACGAGGAGC TGCCCCTCCC GCCCGGGGAC GTGGGGGTCT CCCGGAGTTC GTCCGCCGAG 
CTGGACCGGA GCCGCCCCGC GGTTTCAGTA ACTATTGGTA CTTCAGAGAT GAATGCATTC 
TTGGATGACC CAGAATTTGC CGATATTATG CTGAGAGCAG AGCAAGCAAT AGAAGTTGGA 
ATTTTTCCAG AAAGAATCTC TCAAGGTTCA AGTGGAAGTT ACTTTGTGAA GGATCCTAAG 
AGGAAAATTA TTGGTGTGTT TAAACCCAAA TCAGAAGAGC CTTATGGTCA ACTCAATCCA 
AAATGGACCA AATATGTCCA TAAGGTCTGC TGCCCTTGCT GCTTTGGCCG AGGCTGCCTG 
ATTCCTAATC AGGGGTACCT TTCCGAAGCG GGTGCCTATC TTGTGGACAA CAAGCTTCAT 
CTGAGCATTG TACCTAAAAC AAAGGTGGTT TGGCTTGTCA GTGAGACATT TAACTATAAT 
GCGATTGACC GTGCAAAATC AAGAGGCAAA AAGTATGCTT TAGAAAAAGT GCCAAAAGTG 
GGTAGAAAGT TTCATAGGAT AGGACTCCCT CCTAAGATTG GTTCCTTTCA GTTATTTGTT 
GAAGGTTACA AGGAGGCTGA ATATTGGCTT AGGAAATTTG AAGCTGACCC TTTGCCTGAG 
AATATTAGAA AACAATTTCA GTCACAATTT GAAAGATTAG TTATTTTGGA TTACATCATC 
AGAAATACAG ACAGGGGCAA TGATAATTGG TTAGTCAGAT ACGAAAAGCA GAAATGTGAA 
AAGGAAATTG ACCATAAGGA ATCAAAATGG ATTGATGATG AAGAATTCCT TATTAAAATA 
GCTGCAATTG ATAATGGTCT AGCATTTCCT TTTAAACATC CTGATGAATG GAGAGCATAT 
CCATTTCACT GGGCTTGGCT TCCTCAAGCA AAAGTTCCCT TTTCTGAAGA AATAAGAAAT 
TTGATTCTAC CATATATTTC TGACATGAAC TTTGTGCAAG ATTTATGTGA AGATCTCTAT 
GAACTTTTTA AGACTGACAA AGGATTTGAC AAAGCCACTT TTGAAAGTCA GATGTCTGTG 
ATGAGGGGTC AGATCTTAAA CCTTACTCAG GCATTGAGAG ACGGGAAGAG TCCTTTCCAG 
CTAGTACAGA TACCTTGTGT GATTGTGGAA CGCAGTCAAG GTGGAAGTCA GGGTCGGATT 
GTCCACCTGA GCAATTCCTT TACCCAGACT GTCAATTGCA GGAAGCCATT TTTTTCCTCC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type II PI4-kinases control Weibel-Palade body biogenesis and von Willebrand factor structure in human endothelial cells.
Journal of cell science129(10)2096-105(2016 05)
Lopes da Silva M,O'Connor MN,Kriston-Vizi J,White IJ,Al-Shawi R,Simons JP,Mössinger J,Haucke V,Cutler DF

The high-resolution crystal structure of phosphatidylinositol 4-kinase IIβ and the crystal structure of phosphatidylinositol 4-kinase IIα containing a nucleoside analogue provide a structural basis for isoform-specific inhibitor design.
Acta crystallographica. Section D, Biological crystallography71(Pt 7)1555-63(2015 Jul)
Klima M,Baumlova A,Chalupska D,Hřebabecký H,Dejmek M,Nencka R,Boura E

Type II PtdIns 4-kinase β associates with CD4-p56lck complex and is involved in CD4 receptor signaling.
Molecular and cellular biochemistry395(1-2)231-9(2014 Oct)
Sinha RK,Bojjireddy N,Kanojia D,Subrahmanyam G

Type II phosphatidylinositol 4-kinases interact with FcεRIγ subunit in RBL-2H3 cells.
Molecular and cellular biochemistry390(1-2)197-203(2014 May)
Bojjireddy N,Sinha RK,Subrahmanyam G

Type II phosphatidylinositol 4-kinase β is an integral signaling component of early T cell activation mechanisms.
Biochimie95(8)1560-6(2013 Aug)
Sinha RK,Bojjireddy N,Kulkarni D,Ratheesh A,Chiplunkar SV,Gude R,Subrahmanyam G

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PI4K2B cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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